KR20120107974A - Aad-12 대두 이벤트 416의 검출 - Google Patents

Aad-12 대두 이벤트 416의 검출 Download PDF

Info

Publication number
KR20120107974A
KR20120107974A KR1020127016312A KR20127016312A KR20120107974A KR 20120107974 A KR20120107974 A KR 20120107974A KR 1020127016312 A KR1020127016312 A KR 1020127016312A KR 20127016312 A KR20127016312 A KR 20127016312A KR 20120107974 A KR20120107974 A KR 20120107974A
Authority
KR
South Korea
Prior art keywords
event
seq
soybean
probe
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
KR1020127016312A
Other languages
English (en)
Korean (ko)
Inventor
스티븐 노박
윈싱 코리 추이
토마스 더블유 그린
닝 조우
Original Assignee
다우 아그로사이언시즈 엘엘씨
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 다우 아그로사이언시즈 엘엘씨 filed Critical 다우 아그로사이언시즈 엘엘씨
Publication of KR20120107974A publication Critical patent/KR20120107974A/ko
Abandoned legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00Reactions characterised by their quantitative nature
    • C12Q2545/10Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/114Reactions characterised by their quantitative nature the purpose being quantitative analysis involving a quantitation step
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00Nucleic acid detection characterised by assay method
    • C12Q2561/101Taqman
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10Detection mode being characterised by the assay principle
    • C12Q2565/101Interaction between at least two labels
    • C12Q2565/1015Interaction between at least two labels labels being on the same oligonucleotide
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/166Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020127016312A 2009-11-24 2010-11-24 Aad-12 대두 이벤트 416의 검출 Abandoned KR20120107974A (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US26395009P 2009-11-24 2009-11-24
US61/263,950 2009-11-24
US32736910P 2010-04-23 2010-04-23
US61/327,369 2010-04-23

Publications (1)

Publication Number Publication Date
KR20120107974A true KR20120107974A (ko) 2012-10-04

Family

ID=44212274

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020127016312A Abandoned KR20120107974A (ko) 2009-11-24 2010-11-24 Aad-12 대두 이벤트 416의 검출

Country Status (16)

Country Link
US (1) US8685677B2 (cg-RX-API-DMAC7.html)
EP (1) EP2504456B1 (cg-RX-API-DMAC7.html)
JP (1) JP5907885B2 (cg-RX-API-DMAC7.html)
KR (1) KR20120107974A (cg-RX-API-DMAC7.html)
CN (1) CN102782153B (cg-RX-API-DMAC7.html)
AR (1) AR079150A1 (cg-RX-API-DMAC7.html)
AU (1) AU2010324818B2 (cg-RX-API-DMAC7.html)
CA (1) CA2781622A1 (cg-RX-API-DMAC7.html)
CL (1) CL2012001353A1 (cg-RX-API-DMAC7.html)
CO (1) CO6561770A2 (cg-RX-API-DMAC7.html)
IL (1) IL219932A0 (cg-RX-API-DMAC7.html)
IN (1) IN2012DN04901A (cg-RX-API-DMAC7.html)
MX (1) MX2012006074A (cg-RX-API-DMAC7.html)
RU (1) RU2573898C2 (cg-RX-API-DMAC7.html)
UY (1) UY33058A (cg-RX-API-DMAC7.html)
ZA (1) ZA201203713B (cg-RX-API-DMAC7.html)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102711444B (zh) 2009-11-24 2015-08-12 陶氏益农公司 Aad-12事件416、相关的转基因大豆系及其事件特异性鉴定
WO2016054071A1 (en) 2014-09-29 2016-04-07 Alforex Seeds LLC Low lignin non-transgenic alfalfa varieties and methods for producing the same
WO2018089099A1 (en) 2016-10-11 2018-05-17 Dow Agrosciences Llc Modulation of transgene expression in plants
CN116144671B (zh) * 2022-09-23 2025-07-11 隆平生物技术(海南)有限公司 转基因玉米事件lp026-3及其检测方法
CN116676417B (zh) * 2023-07-24 2023-09-29 捷康生物科技(海南)有限公司 转基因大豆事件jk1001-3及其检测方法
CN116694812B (zh) * 2023-07-25 2023-10-03 隆平生物技术(海南)有限公司 转基因大豆事件lp086-2及其检测方法
CN116694813B (zh) * 2023-07-25 2023-10-03 隆平生物技术(海南)有限公司 转基因大豆事件lp086-1及其检测方法
CN116694815B (zh) * 2023-08-01 2023-10-03 隆平生物技术(海南)有限公司 转基因大豆事件lp012-2及其检测方法
CN116694814B (zh) * 2023-08-01 2023-10-10 隆平生物技术(海南)有限公司 转基因大豆事件lp012-1及其检测方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060127889A1 (en) * 2000-07-25 2006-06-15 Dotson Stanton B Method for assessing transgene expression and copy number
CN1370841A (zh) * 2001-02-26 2002-09-25 安利忻 基因改造农作物及其加工产品的定量检测方法和试剂盒
US7955800B2 (en) * 2002-06-25 2011-06-07 Advpharma Inc. Metastasis-associated gene profiling for identification of tumor tissue, subtyping, and prediction of prognosis of patients
RU2270254C2 (ru) * 2004-04-30 2006-02-20 Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук Способ идентификации трансгенных последовательностей днк в растительном материале и продуктах на его основе, набор олигонуклеотидов и биочип для осуществления этого способа
AU2006308959B2 (en) * 2005-10-28 2012-09-06 Corteva Agriscience Llc Novel herbicide resistance genes
ES2569940T3 (es) * 2007-04-20 2016-05-13 Sigma-Tau Rare Disease Ltd Adenosina desaminasa recombinante estable
CN102711444B (zh) * 2009-11-24 2015-08-12 陶氏益农公司 Aad-12事件416、相关的转基因大豆系及其事件特异性鉴定

Also Published As

Publication number Publication date
AR079150A1 (es) 2011-12-28
ZA201203713B (en) 2013-01-30
RU2573898C2 (ru) 2016-01-27
AU2010324818B2 (en) 2015-09-17
JP5907885B2 (ja) 2016-04-26
CN102782153B (zh) 2015-08-12
EP2504456A1 (en) 2012-10-03
MX2012006074A (es) 2012-06-19
RU2012126088A (ru) 2013-12-27
US20130029329A1 (en) 2013-01-31
UY33058A (es) 2011-06-30
JP2013512661A (ja) 2013-04-18
AU2010324818A1 (en) 2012-06-14
CO6561770A2 (es) 2012-11-15
CA2781622A1 (en) 2011-06-03
CN102782153A (zh) 2012-11-14
CL2012001353A1 (es) 2012-10-05
US8685677B2 (en) 2014-04-01
EP2504456A4 (en) 2014-04-09
IL219932A0 (en) 2012-07-31
EP2504456B1 (en) 2017-12-20
IN2012DN04901A (cg-RX-API-DMAC7.html) 2015-09-25

Similar Documents

Publication Publication Date Title
JP5771208B2 (ja) Aad−1事象das−40278−9の検出
KR101906605B1 (ko) Aad-12 이벤트 416, 관련된 트랜스제닉 대두 계통, 및 이의 이벤트-특이적 확인
WO2011066360A1 (en) Detection of aad-12 soybean event 416
RU2573898C2 (ru) Детектирование aad-12-события 416 у сои
JP2007513641A (ja) トウモロコシ植物mon88017および組成物ならびにその検出方法
US20110151441A1 (en) Endpoint taqman methods for determining zygosity of corn comprising tc1507 events
US9204599B2 (en) Detection of AAD1 event DAS-40278-9
AU2011311955B9 (en) Endpoint TaqMan methods for determining zygosity of cotton comprising Cry 1F event 281-24-236
US9534260B2 (en) Materials and methods for detecting the aryloxyalkanoate dioxygenase gene (AAD-12) containing event pDAB4472-1606 in plants
EP2467500B1 (en) Detection of aad-1 event das-40278-9
EP2768981B1 (en) Method to determine zygosity of the fad2 gene in canola using end-point pcr
WO2012048136A1 (en) Endpoint taqman methods for determining zygosity of cotton comprising cry1ac event 3006-210-23
HK1173194B (en) Detection of aad-1 event das-40278-9
BR112012003873B1 (pt) Método para determinar zigosidade de uma planta de milho compreendendo um transgene de ariloxialcanoato dioxigenase (aad-1) como encontrado no evento de milho 5 das-40278-9, e kit para realizar o referido método

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20120622

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20151111

Comment text: Request for Examination of Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20170201

Patent event code: PE09021S01D

AMND Amendment
E601 Decision to refuse application
PE0601 Decision on rejection of patent

Patent event date: 20170820

Comment text: Decision to Refuse Application

Patent event code: PE06012S01D

Patent event date: 20170201

Comment text: Notification of reason for refusal

Patent event code: PE06011S01I

AMND Amendment
PX0901 Re-examination

Patent event code: PX09011S01I

Patent event date: 20170820

Comment text: Decision to Refuse Application

Patent event code: PX09012R01I

Patent event date: 20170403

Comment text: Amendment to Specification, etc.

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20171011

Patent event code: PE09021S01D

AMND Amendment
PX0701 Decision of registration after re-examination

Patent event date: 20180423

Comment text: Decision to Grant Registration

Patent event code: PX07013S01D

Patent event date: 20171122

Comment text: Amendment to Specification, etc.

Patent event code: PX07012R01I

Patent event date: 20170920

Comment text: Amendment to Specification, etc.

Patent event code: PX07012R01I

Patent event date: 20170820

Comment text: Decision to Refuse Application

Patent event code: PX07011S01I

Patent event date: 20170403

Comment text: Amendment to Specification, etc.

Patent event code: PX07012R01I

X701 Decision to grant (after re-examination)
PC1904 Unpaid initial registration fee