IN2012DN04901A - - Google Patents

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Publication number
IN2012DN04901A
IN2012DN04901A IN4901DEN2012A IN2012DN04901A IN 2012DN04901 A IN2012DN04901 A IN 2012DN04901A IN 4901DEN2012 A IN4901DEN2012 A IN 4901DEN2012A IN 2012DN04901 A IN2012DN04901 A IN 2012DN04901A
Authority
IN
India
Prior art keywords
subject
event
relates
assays
soybean
Prior art date
Application number
Inventor
Novak Stephen
Cory Cui Yunxing
W Greene Thomas
Zhou Ning
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Priority claimed from PCT/US2010/057967 external-priority patent/WO2011066360A1/en
Publication of IN2012DN04901A publication Critical patent/IN2012DN04901A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/114—Reactions characterised by their quantitative nature the purpose being quantitative analysis involving a quantitation step
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/101—Taqman
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
    • C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates in part to methods of detecting an AAD-12 soybean event. The subject invention provides assays for detecting the presence of the subject event in a sample (of soybeans, for example). Kits and conditions useful in conducting the assays are also provided. More specifically, the present invention relates in part to an endpoint TaqMan PCR assay for the AAD-12 soybean event. Some embodiments are directed to assays that are capable of high throughput zygosity analysis. The subject invention further relates, in part, to the discovery of a preferred reference gene for use in determining zygosity. This invention also relates in part to plant breeding using any of the subject methods. In some embodiments, said event / polynucleotide sequence can be 'stacked' with other traits. The subject procedures can be used to uniquely identify soybean lines comprising the event of the subject invention.
IN4901DEN2012 2009-11-24 2010-11-24 IN2012DN04901A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US26395009P 2009-11-24 2009-11-24
US32736910P 2010-04-23 2010-04-23
PCT/US2010/057967 WO2011066360A1 (en) 2009-11-24 2010-11-24 Detection of aad-12 soybean event 416

Publications (1)

Publication Number Publication Date
IN2012DN04901A true IN2012DN04901A (en) 2015-09-25

Family

ID=44212274

Family Applications (1)

Application Number Title Priority Date Filing Date
IN4901DEN2012 IN2012DN04901A (en) 2009-11-24 2010-11-24

Country Status (16)

Country Link
US (1) US8685677B2 (en)
EP (1) EP2504456B1 (en)
JP (1) JP5907885B2 (en)
KR (1) KR20120107974A (en)
CN (1) CN102782153B (en)
AR (1) AR079150A1 (en)
AU (1) AU2010324818B2 (en)
CA (1) CA2781622A1 (en)
CL (1) CL2012001353A1 (en)
CO (1) CO6561770A2 (en)
IL (1) IL219932A0 (en)
IN (1) IN2012DN04901A (en)
MX (1) MX2012006074A (en)
RU (1) RU2573898C2 (en)
UY (1) UY33058A (en)
ZA (1) ZA201203713B (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112012012511A2 (en) 2009-11-24 2015-09-15 Dow Agrosciences Llc event 416 of the aad-12 gene related to transgenic soybean strains and their event-specific identification
AU2015323919B2 (en) 2014-09-29 2019-01-31 Dlf Usa Inc. Low lignin non-transgenic alfalfa varieties and methods for producing the same
WO2018089099A1 (en) 2016-10-11 2018-05-17 Dow Agrosciences Llc Modulation of transgene expression in plants
CN116676417B (en) * 2023-07-24 2023-09-29 捷康生物科技(海南)有限公司 Transgenic soybean event JK1001-3 and detection method thereof
CN116694812B (en) * 2023-07-25 2023-10-03 隆平生物技术(海南)有限公司 Transgenic soybean event LP086-2 and detection method thereof
CN116694813B (en) * 2023-07-25 2023-10-03 隆平生物技术(海南)有限公司 Transgenic soybean event LP086-1 and detection method thereof
CN116694814B (en) * 2023-08-01 2023-10-10 隆平生物技术(海南)有限公司 Transgenic soybean event LP012-1 and detection method thereof
CN116694815B (en) * 2023-08-01 2023-10-03 隆平生物技术(海南)有限公司 Transgenic soybean event LP012-2 and detection method thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060127889A1 (en) * 2000-07-25 2006-06-15 Dotson Stanton B Method for assessing transgene expression and copy number
CN1370841A (en) * 2001-02-26 2002-09-25 安利忻 Quantitative test method and reagent kit for genetically modified crop and relevant product
US7955800B2 (en) * 2002-06-25 2011-06-07 Advpharma Inc. Metastasis-associated gene profiling for identification of tumor tissue, subtyping, and prediction of prognosis of patients
RU2270254C2 (en) * 2004-04-30 2006-02-20 Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук Identification of transgenic dna sequences in plant material and products made of the same, oligonucleotide kit and bioarray therefor
EP1947926B1 (en) 2005-10-28 2014-11-26 Dow AgroSciences LLC Novel herbicide resistance genes
HUE027603T2 (en) * 2007-04-20 2016-10-28 Sigma-Tau Rare Disease Ltd Stable recombinant adenosine deaminase
BR112012012511A2 (en) * 2009-11-24 2015-09-15 Dow Agrosciences Llc event 416 of the aad-12 gene related to transgenic soybean strains and their event-specific identification

Also Published As

Publication number Publication date
CA2781622A1 (en) 2011-06-03
RU2012126088A (en) 2013-12-27
US20130029329A1 (en) 2013-01-31
UY33058A (en) 2011-06-30
EP2504456A4 (en) 2014-04-09
AR079150A1 (en) 2011-12-28
CO6561770A2 (en) 2012-11-15
CN102782153A (en) 2012-11-14
AU2010324818B2 (en) 2015-09-17
JP2013512661A (en) 2013-04-18
MX2012006074A (en) 2012-06-19
IL219932A0 (en) 2012-07-31
US8685677B2 (en) 2014-04-01
JP5907885B2 (en) 2016-04-26
EP2504456B1 (en) 2017-12-20
EP2504456A1 (en) 2012-10-03
AU2010324818A1 (en) 2012-06-14
RU2573898C2 (en) 2016-01-27
CL2012001353A1 (en) 2012-10-05
KR20120107974A (en) 2012-10-04
ZA201203713B (en) 2013-01-30
CN102782153B (en) 2015-08-12

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