KR20120059492A - 주형 ｄｎａ의 복제, 증폭 및 서열화 방법 - Google Patents

주형 ｄｎａ의 복제, 증폭 및 서열화 방법 Download PDF

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Publication number
KR20120059492A
KR20120059492A KR1020127002885A KR20127002885A KR20120059492A KR 20120059492 A KR20120059492 A KR 20120059492A KR 1020127002885 A KR1020127002885 A KR 1020127002885A KR 20127002885 A KR20127002885 A KR 20127002885A KR 20120059492 A KR20120059492 A KR 20120059492A
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South Korea
Prior art keywords
amplification
dna
dna polymerase
ammonium
concentration
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English (en)
Korean (ko)
Inventor
마가리타 살라스 팔그라스
미구엘 드 베가 호세
호세 엠 라자로 볼로스
루이스 블랑코 다빌라
마리오 멘시아 카발레로
Original Assignee
콘세호 수페리오르 데 인베스티가시오네스 시엔티피카스
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Publication of KR20120059492A publication Critical patent/KR20120059492A/ko
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020127002885A 2009-07-02 2010-07-02 주형 ｄｎａ의 복제, 증폭 및 서열화 방법 Withdrawn KR20120059492A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ES200930412A ES2351294B8 (es) 2009-07-02 2009-07-02 Metodo para la replicación, amplificación, o secuenciación de un adn molde.
ESP200930412 2009-07-02
PCT/ES2010/070456 WO2011000998A2 (es) 2009-07-02 2010-07-02 Método para la replicación, amplificación o secuenciación de un adn molde

Publications (1)

Publication Number Publication Date
KR20120059492A true KR20120059492A (ko) 2012-06-08

Family

ID=43411512

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020127002885A Withdrawn KR20120059492A (ko) 2009-07-02 2010-07-02 주형 ｄｎａ의 복제, 증폭 및 서열화 방법

Country Status (17)

Country Link
US (1) US20120178092A1 (https=)
EP (1) EP2450453B1 (https=)
JP (1) JP2012531221A (https=)
KR (1) KR20120059492A (https=)
CN (1) CN102648289A (https=)
AU (1) AU2010267955A1 (https=)
BR (1) BRPI1014001A2 (https=)
CA (1) CA2766728A1 (https=)
CL (1) CL2011003313A1 (https=)
CO (1) CO6480969A2 (https=)
DK (1) DK2450453T3 (https=)
ES (1) ES2351294B8 (https=)
IL (1) IL217325A0 (https=)
IN (1) IN2012DN00172A (https=)
MX (1) MX2012000152A (https=)
SG (1) SG177411A1 (https=)
WO (1) WO2011000998A2 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201112140D0 (en) * 2011-07-14 2011-08-31 Dna Electronics Nucleic acid amplification
CN105483115A (zh) * 2014-09-19 2016-04-13 深圳华大基因科技有限公司 一种用于单细胞全基因组扩增的试剂盒及其应用
CN104328111A (zh) * 2014-10-29 2015-02-04 北京大学 一种用于测序的高通量扩增质粒的方法
CN105695571A (zh) * 2016-01-29 2016-06-22 苏州金唯智生物科技有限公司 一种基于滚环扩增的dna定量方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198543A (en) * 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5001050A (en) 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US20030049614A1 (en) * 1999-10-06 2003-03-13 Holly Hurlbut Hogrefe Compositions for dna amplification, synthesis, and mutagenesis
US6323009B1 (en) * 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
JP2006513705A (ja) * 2003-02-05 2006-04-27 アマシャム・バイオサイエンス・コーポレイション 核酸増殖
JP4918409B2 (ja) * 2006-07-26 2012-04-18 西川ゴム工業株式会社 核酸配列の増幅方法
EP2108048A1 (en) * 2007-01-17 2009-10-14 Meridian Bioscience, Inc. Stable reagents and kits useful in loop-mediated isothermal amplification (lamp)
GB0901593D0 (en) * 2009-01-30 2009-03-11 Touchlight Genetics Ltd Production of closed linear DNA

Also Published As

Publication number Publication date
AU2010267955A1 (en) 2012-02-16
JP2012531221A (ja) 2012-12-10
SG177411A1 (en) 2012-02-28
US20120178092A1 (en) 2012-07-12
MX2012000152A (es) 2012-06-08
IN2012DN00172A (https=) 2015-04-17
ES2351294B8 (es) 2012-06-06
CL2011003313A1 (es) 2012-06-08
WO2011000998A2 (es) 2011-01-06
CA2766728A1 (en) 2011-01-06
WO2011000998A3 (es) 2011-07-21
CO6480969A2 (es) 2012-07-16
CN102648289A (zh) 2012-08-22
DK2450453T3 (en) 2017-08-21
ES2351294A1 (es) 2011-02-02
EP2450453A4 (en) 2012-12-26
ES2351294B1 (es) 2011-11-24
IL217325A0 (en) 2012-02-29
BRPI1014001A2 (pt) 2018-01-30
EP2450453A2 (en) 2012-05-09
EP2450453B1 (en) 2017-05-31

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