KR20120044580A - Foot cleansing composition containing immobilized protease - Google Patents

Abstract

PURPOSE: A foot cleansing composition is provided to cause physical scrub function by microbeads and chemical decomposing function by protease, thereby improving exfoliating ability and metabolism. CONSTITUTION: A foot cleansing composition contains microbeads in which proteinase is fixed. The proteinase is one or more mixture selected from papain, bromelain and pancreatin. The amount of the microbeads is 0.1-10 weight% on the basis of the total weight of the composition. The microbeads are circular porous microbeads manufactured by chitosan. The composition additionally comprises one or more components selected from surfactant, moisturizer, antiseptic, antioxidant, antimycotic, perfume, pigment, thickener, moisture carrier, and mixture thereof.

Description

Foot cleansing composition containing immobilized protease

The present invention relates to a foot washing composition, and more particularly, to a foot washing composition comprising an immobilized protease.

The surface of the skin is surrounded by a stratum corneum, which prevents the escape of substances in the body and protects the body's interior from external physical and chemical stimuli. The epidermis of the skin is turned over to the basal layer, the pole layer, the granule layer and the stratum corneum and eventually removed as waste products, which takes 14 days from the base layer to the granule layer and 14 days from the excess to the outermost stratum corneum. It takes However, as the skin ages, the turnover period generally increases, and as a result, the proportion of the stratum corneum that occupies the outermost part increases, resulting in a dry and dull skin. In addition, the growth of bacteria between the stratum corneum increases and odor may occur due to decay. Therefore, in order to maintain the health of the skin to prevent excessive accumulation of keratin. In particular, the foot is thicker than other skin areas with 10 ~ 20 stratum corneum, forming 45 ~ 50 layers, which can be a hotbed of bad smell. If attached, it is easy to spread throughout the sole, so special care should be taken.

Exfoliants have been developed in a variety of ways, including chemical and physical removers. Typical chemical removers include alpha-hydroxy acid and beta-hydroxy acid, which are combined with other components rather than alone to be used in exfoliating products for foot care. In addition, papain, pancreatin, and the like were used in the form of powder as the enzyme. Scrub is a typical physical remover.

Products containing scrubs are very effective in cleaning and removes various wastes, dust and cosmetic residues accumulated on the skin. However, in the case of the inorganic scrub, the hardness is high and the surface is not rounded, so that there is a problem that can damage the skin finely. Therefore, as an alternative to this, the use of enzymes for cleaning compositions is increasing.

Enzymes are biocatalysts that have the advantages of being environmentally friendly and superior in substrate specificity over conventional cleaning agents. Nevertheless, when formulated in an aqueous solution, the stability is limited to use as a raw material for cosmetics.

It is an object of the present invention to provide an environment-friendly composition for washing feet containing protease with improved stability.

Still another object of the present invention is to provide a composition for washing feet that is harmless to humans by minimizing side effects.

The invention according to claim 1 is a composition for washing feet, and contains microbeads to which proteolytic enzymes are immobilized.

According to the composition for foot cleaning according to claim 1, the physical scrub action by the microbead and the chemical degradation action by the protease can occur together, causing a synergistic effect upon keratin degradation, and promoting metabolism. It can help keep you clean and your feet healthy.

The invention according to claim 2 is the foot washing composition according to claim 1, wherein the proteolytic enzyme is one or a mixture of two or more selected from the group consisting of papain, bromelain and pancreatin.

According to the foot-washing composition according to claim 2, papain, bromelain and pancreatin are immobilized to exhibit more excellent keratin effect.

The invention according to claim 3 is the composition for foot washing according to claim 1, wherein the microbeads to which proteolytic enzymes are immobilized are contained at 0.1 to 10% by weight based on the weight of the whole composition.

According to the composition for feet cleaning of Claim 3, it shows high washing | cleaning effect and exfoliation effect, without excessively containing and causing a skin trouble.

The invention according to claim 4 is the foot washing composition according to claim 1, wherein the microbeads are round porous microbeads made of chitosan.

According to the composition for feet cleaning according to claim 4, since it is made of porous microbeads using a natural-derived functional material, there is no side effect and the effect of the physical scrub can be further enhanced. In addition, chitosan-made microbeads are harmless to the human body and have a round shape to enhance usability.

The invention according to claim 5 is the composition for foot cleaning according to claim 1, wherein the composition for foot cleaning is a surfactant, a humectant, a preservative, an antioxidant, an antifungal agent, a perfume, a pigment, a viscosity increasing agent, and water. And one or more components selected from the group consisting of carriers and mixtures thereof.

According to the composition for feet cleaning of Claim 5, there exists an effect which increases a usability, skin stability, etc. in addition to a foot exfoliation effect.

The invention according to claim 6 is the foot cleaning composition according to any one of claims 1 to 5, wherein the foot cleaning composition is in the form of a shampoo, cream, massage cream, lotion, gel, ointment or spray. to be.

According to the foot-washing composition of Claim 6, it can be used according to the objective according to a user's taste, a use part, a user's condition, etc.

As described above, the foot cleaning composition according to the present invention causes a synergistic effect upon keratin degradation by physical scrub action by microbeads and a chemical decomposition action by proteolytic enzymes and promotes metabolism to maintain cleanliness and foot health. It can help. In addition, papain, bromelain, and pancreatin can be usefully used as a foot cleanser by showing excellent exfoliation effect when immobilized.

1 is a schematic diagram of preparing microbeads according to an embodiment of the present invention.
Figure 2 is a micrograph of the surface of the microbeads before and after proteolytic enzyme binding according to an embodiment of the present invention.
3A to 3C show FT-IR Spectrum of chitosan microbeads, chitosan microbeads after glutaraldehyde reaction, and chitosan microbeads to which proteases are bound, respectively.
4 and 5 are photographs taken of the state of the foot before and after use of the foot cleaning composition according to an embodiment of the present invention.
6 is a graph comparing the activity stabilization of free protein enzyme and immobilized protein enzyme.

Hereinafter, the present invention will be described in more detail.

Foot cleaning composition according to an embodiment of the present invention contains a microbead fixed with a proteolytic enzyme.

The protease is a catalyst having an exfoliating function, which can be any of them, but those derived from plants or animals that have no problem of skin irritation, for example, papain obtained from papaya and bromelain obtained from pineapple. Pancreatin from pig bile can be used.

The microbeads can be any that is suitable for immobilizing the enzyme, for example, animal scrubs such as crab shells, egg shells, apricot seeds, almond seeds, walnut shells, balsam seeds, july seeds, nylon scrubs, nylon , Organic scrubs such as polyvinyl chloride, polystyrene, polypropylene, polyethylene, cellulose, and inorganic scrubs such as silica, alumina, and calcium carbonate can be used. It is preferable to make it. When chitosan is used as a scrub agent, the size and shape are important. Too large or brittle particles are not preferable, and therefore, it is preferable to use round microbeads. By combining proteolytic enzymes on the surface of the microbeads, physical scrub and chemical degradation can occur together.

The microbead fixed with the protease is 0.1 to 10% by weight, preferably 1.0 to 5% by weight based on the total weight of the composition. Exceeding 10% by weight can cause a problem of skin sensitization due to excessive removal of keratin, and when contained below 0.1% by weight, no effective exfoliation effect can be obtained.

Foot cleaning composition according to an embodiment of the present invention may further include an additive usually used in cosmetic formulations. Examples include surfactants for cleaning, penetration, foaming, moisturizers for proper retention of moisture, antioxidants to prevent or delay oxidation, fragrances to mask the odor of the raw materials or impart an appropriate odor, and the pearl effect. A pulping agent to impart, a smooth feel and proper oiliness, high-grade alcohol, a metal ion sequestrant to prevent the formation of scales, a preservative to prevent excessive growth of microorganisms, an antifungal agent, and a viscosity increasing agent [1] R. J Akers, Foams, Academic Press, New York (1976). 2) JJ Bikerman, Foam, Springer, Berlin (1973)].

The surfactants include nonionic and amino or amphoteric surfactants. In the case of nonionic systems, there is a limit to finally perform the object of the present invention because of its low cleaning power and low usability. Therefore, it is preferable to mix | blend an amino acid system and an amphoteric system independently, or to mix and use these and nonionic systems.

The nonionic surfactant is not strictly limited, but in general, HLB 6-12 is mixed well with oil, and when washed with water, it becomes white turbid and easily washed away with PJ-30 sorbitan tetraoleate, PIG- 30 sorbitan tetraisostearate, Fiji-20 glyceryl triisostearate are suitable but not limited to one or two or more kinds thereof.

Anionic surfactants include sodium Cocoyl Isethionate, alkyl polysaccharides, lactobionamides, ethylene glycol esters, glycerol monoethers, polyhydroxyamides (glucamides), primary and secondary Alcohol ethoxylates and the like.

Amino acid surfactants include acyl alanate, acyl glycinate and acyl glutamate, preferably disodium acyl glutamate, potassium cocoyl glycinate and the like.

The moisturizing agents include 1,3-butylene glycol, propylene glycol, and glycerin, and preservatives include methyl paraben, ethyl paraben, propyl paraben, and butyl paraben, and the thickener is cetearyl alcohol ( Cetearyl alcohol, carboxy vinyl polymer, and Xanthan Gum, and the fragrance includes essential oil, and Sorbitan Olivate as an emulsifier. The above examples are listed for illustrative purposes, and any one used in the art may be appropriately selected.

The pulsing agent is used to impart a feeling of pearl to make the appearance gorgeous in a cleaning agent or cosmetics, and conventionally known pulsing agents include ethylene glycol, propylene glycol, oligomers and monostearate or distearate of alkylene glycol. Of these, ethylene glycol, mono stearate and distearate are mainly used.

Foot cleaning composition according to the present invention may add salt or sun salt to reinforce the athlete's foot prevention function.

The foot cleaning composition according to the present invention is not particularly limited in the formulation, and may be prepared and used, for example, in the form of a shampoo, cream, massage cream, lotion, gel, ointment or spray.

Hereinafter, the present invention will be described in detail with reference to the drawings.

Referring to FIG. 1, according to one embodiment of the present invention, the microbeads may be prepared using a W / 0 emulsion. In addition to chitosan, animal scrubs such as egg shells, vegetable scrubs such as apricot seeds, organic scrubs such as nylon, and inorganic scrubs such as silica can be physically pulverized and used as beads. Chitosan is suitable for enzyme immobilization because it has a primary amine group. In other words, chitosan is preferable because it can be easily dissolved and processed into round particles when an organic acid is added.

In the case of chitosan extracted from crab shell, a W / O emulsion is prepared by mixing an organic acid in which chitosan is dissolved as an aqueous phase and a nonpolar organic solvent as an organic phase. At this time, to form a stable water-in-oil emulsion (W / O), the chitosan solution is slowly added while stirring at 200-450 rpm for 1 to 2 hours. Thereafter, the basic solution is added to cure to form coacervate. Microbeads are then prepared by layer separation, curing, filtration, washing and drying.

Ascorbic acid, glycolic acid, lactic acid, acetic acid, trifluoroacetic acid may be used as the organic acid, preferably acetic acid may be used. The organic acid is preferably used at a concentration of 0.5 ~ 2%.

As the nonpolar organic solvent, n-hexane, cyclohexane, dichloromethane, dichloroethane may be used, and preferably toluene may be used.

As the basic solution, sodium hydroxide, potassium hydroxide, lithium hydroxide, calcium hydroxide, monoethanolamine, diethanolamine, triethanolamine may be used, and sodium hydroxide may be preferably used. have. The basic solution is preferably used at a concentration of 5-15%.

The washing, filtration and drying methods may be appropriately selected and used by those skilled in the art using techniques known in the art.

Next, the enzyme is immobilized on the microbead according to an embodiment of the present invention.

The prepared microbeads are placed in a biological adhesive and reacted. For example, 40 to 60 mg of microbeads are added to 1 ml of glutaraldehyde solution and reacted for 1 to 3 hours. After the reaction, washing with hot distilled water removes the smell of glutaraldehyde from the microbeads. At this time, the step of washing with hot distilled water is very important. This step completely removes the unreacted material and then binds the enzyme in the presence of a buffer solution, thereby minimizing side reactions caused by unreacted glutaraldehyde. Again, the microbeads are stabilized by washing with a buffer solution such as phosphate buffer (pH 7.0). Again, 1 ~ 2mg of protease is added to 1ml of buffer solution and immobilized at 18 ~ 28 ℃ and 200 ~ 300rpm in shaking incubator for 4 ~ 8 hours.

Thereafter, the solution is filtered and washed with distilled water to complete the proteolytic enzyme-bound microbeads. The surface of the bead to which the protease is immobilized can be observed through an electron microscope (see FIGS. 3A to 3C).

Next, the foot washing composition containing the microbead fixed with the protease is prepared by the following method.

The protease-immobilized microbeads are configured to contain 1 to 10% by weight based on the total weight of the composition. At this time, it may be configured to include a surfactant, a moisturizing agent, antioxidants, preservatives and the like.

The optimal temperature for using the foot cleaning composition thus prepared is 30 ~ 45 ℃, pH range is 5 ~ 9. Preferably the temperature of 35-40 degreeC, and optimal pH are 5-7. This is because proteolytic enzymes penetrate into dead keratinocytes in warm hot water and naturally decompose, thereby fundamentally solving the problem of keratin, and increasing scrubbing and moisturizing effects. If the temperature is too high, the enzyme may die or damage the skin.

Through the following examples will be described the present invention in more detail. This embodiment is intended to illustrate the present invention in more detail, but the scope of the present invention is not limited thereto.

Example  One: Microbead  Produce

Chitosan was dissolved in 250 ml of 1% acetic acid aqueous solution and left in the refrigerator for about one day to remove bubbles. 2 g of sorbitan monooleate (trade name Span80, HLB = 4.3) was added as an emulsifier and 1.2 ml of n-hexane as an auxiliary emulsifier. Slowly added. Stir for about 2 hours to form a stable spherical water-in-oil emulsion (W / O), then harden with 1.5 liter of 10% sodium hydroxide aqueous solution and wash with boiling distilled water until the filtrate becomes neutral. After washing with ethanol, ether, acetone in order and dried in a dryer.

Example  2. Protease-immobilized Microbead  Produce

4 ml of 10% glutaraldehyde solution was added to 200 mg of the microbead prepared in Example 1 and reacted for 2 hours. After the reaction, the beads were washed with hot distilled water until no smell of glutaraldehyde was obtained. Finally washed twice with 0.05 mole phosphate buffer (pH 7.0). Then, 4ml of phosphate buffer bath and 1.5mg of protease (papain) were added to the shaking incubator together with the microbeads and fixed at 25 ° C. and 250rpm for 6 hours. After the immobilization was completed, the solution was washed with filtered distilled water and mixed with the base of the cleaning cosmetics. The surface of the immobilized beads was observed through an electron microscope (Hitachi, Japan) and the chemical bond was confirmed by FT-IR (see FIG. 2).

Example  3: Preparation of Foot Washing Composition

1 g of purified water, 1 g of immobilized enzyme, 20 g of disodium acylglutamate, 20 g of sodium cocoyl isethionate, and 10 g of potassium cocoylglycinate were mixed by heating with a water bath. It was. 1 g of ethylene glycol distearate and 5 g of 1,3-butylene glycol were mixed at 55 to 65 ° C. 0.3 g of cetearyl alcohol, 3 g of sorbitan olivate (Sorbitan Olivate), and 0.05 g of ethylene diamine tetraacetic acid (disodium salt) were mixed at 50 to 55 ° C. As a preservative, 0.01 g of methyl paraben was mixed at 45 ° C. to prepare a foot washing composition.

Experimental Example  1: Enzyme Immobilization Confirmation

In order to observe the immobilization, the shape of the bead surface before and after the immobilization was observed at a magnification of 50 times and 20,000 using a scanning electron microscope (SEM). The results are shown in FIG. As observed in the micrograph of FIG. 2, it was possible to observe the shape of a fairly flat bead surface before enzyme immobilization, but it was found that the enzyme had a rough shape after fixing the enzyme. This may be due to the physical change of the surface during the immobilization process, but it can be seen that the enzyme is immobilized due to the binding of other substances on the surface.

In addition, after reacting glutaraldehyde to chitosan microbeads as shown in the FT-IR spectrum of FIG. 3 (FIGS. 3A to 3C), a peak due to aldehyde appeared at 1716 cm and an imine at 1100 cm. The intensity of the peaks increased. After the immobilization of the enzyme, it was confirmed that the enzyme was immobilized by chemical bonding because the peak caused by the aldehyde disappeared again by the reaction between the aldehyde and the enzyme.

Experimental Example  2: confirm the efficacy of the foot cleaning composition of the present invention

In order to confirm the efficacy effect on the composition of the present invention, usability review of the composition of Example 3 was carried out for 7 days by the Home Usage Test (HUT) test. Each reviewer was asked to use the sample at 8 to 10 o'clock every evening according to the prescribed method, and the questionnaire observed the condition of the sole including the heel after 1 hour after 7 days of use. Before using the composition according to Example 1, the monochlorochloride (phosphorescent material) and petrolatum were mixed to dye the sole of the foot, and then washed with the composition for washing feet according to Example 1 in warm water. The state before and after washing with the foot washing composition according to Example 1 was photographed at a UV lamp 365nm and shown in FIG. 4. In addition, the state of the bottom of the foot before and after using the foot cleaning composition according to Example 1 was shown in Fig. 5 by taking a picture.

As shown in Figures 4 and 5, it was found that the use of the foot cleaning composition containing the proteolytic enzyme-immobilized microbeads, the overall condition of the soles, including the heel. This can be seen that the stabilized proteolytic enzyme reacts with the keratin on the surface of the skin to decompose it, and at the same time, the scrubbing effect of the micromead is added, leading to the exfoliation of the keratin.

Experimental Example  3: Activity Comparison between Free Protease and Immobilized Protease

The papain immobilized on the chitosan microbead prepared in Example 2 and the activity of free papain were observed for 1 to 30 days. Papain activity was measured by UV spectrophotometer. Substrate solution was dissolved in N-benzoyl-DL-arginine-4-nitroanilide in 0.5 mole phosphate buffer (pH7.0) to the reactor containing the immobilization carrier and reacted for 10 minutes at room temperature immediately in the ice bath After addition, the filtrate was aliquoted and measured by absorbance at 405 nm. The results are shown in FIG.

As shown in Figure 6 it can be seen that the immobilized protease is continuously stable activity.

Claims (6)

A foot washing composition containing microbeads fixed with proteolytic enzymes. The method of claim 1,
The proteolytic enzyme is one or two or more mixtures selected from the group consisting of papain, bromelain and pancreatin. The method of claim 1,
Foot composition for the microbeads is 0.1 to 10% by weight based on the total weight of the composition. The method of claim 1,
The microbeads are round porous microbeads made of chitosan. The method of claim 1,
The foot cleaning composition further comprises one or two or more components selected from the group consisting of surfactants, moisturizers, preservatives, antioxidants, antifungals, fragrances, pigments, viscosity increasing agents, water carriers and mixtures thereof. Foot cleaning composition. The method according to any one of claims 1 to 5,
The foot cleaning composition is a foot cleaning composition in the form of a shampoo, cream, massage cream, lotion, gel, ointment or spray.

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