KR100507960B1 - Composition of removing corneum, method of preparing the same and cleansing composition including the same - Google Patents

Abstract

Disclosed is a composition for removing keratin, a method for producing the keratin and the like, and a facial cleansing composition comprising such a protease coating composition. Skin keratin comprising 40 to 50 parts by weight of the seed layer, 0.00001 to 10.0 parts by weight of an enzyme layer formed by coating a proteolytic enzyme on the seed layer, and 0.5 to 10.0 parts by weight of a stabilizer layer formed by coating a betaine on the enzyme layer. It provides a removal composition and a face wash composition prepared using the same. And the skin exfoliation composition is dissolved in ethanol and coated on the seed to form a coating seed layer, coating the proteolytic enzyme on the coating seed layer to form an enzyme layer and then stable by coating a betaine on the enzyme layer It is manufactured by forming a topical layer. The protein exfoliation effect of the enzyme is kept constant regardless of external influences such as moisture or heat, so that the exfoliation composition excellent in exfoliation effect can be prepared, and the unnecessary keratin components of the outermost layer of the skin and pores on the skin surface, etc. It is possible to provide a skin cleansing composition that can effectively remove the impurities present.

Description

Composition for removing exfoliation of skin, preparation method thereof, and facial cleansing composition comprising the same {COMPOSITION OF REMOVING CORNEUM, METHOD OF PREPARING THE SAME AND CLEANSING COMPOSITION INCLUDING THE SAME}

The present invention relates to proteolytic enzymes. More specifically, the present invention relates to a proteolytic enzyme that can effectively and continuously exfoliate skin and a skin cleansing composition using the same.

The corneum is a derivative of the Latin cornu, which means that the original meaning is fiery hard. In addition, the stratum corneum is the outermost or upper layer of the epidermal layer. It is sometimes referred to as a cellular upper layer in the sense of a layer, an outermost layer, or Latin, meaning a layer of horn, a hard layer of horns, or an upper layer of a cell layer.

The stratum corneum is formed by keratinizing the cells that reach the outer surface of the skin. Keratinization means that the cells that reach the stratum corneum turn into hard and flat dead cells. The stratum corneum leaves the dead cells away from the skin. This is done by stacking layers.

Millions of dead keratinocytes are removed from the skin and replaced with new keratinocytes in one day. Looking at the process of skin keratinization, keratinocytes produced in the basal layer take about 14 days to the granular layer. The cells of the basal layer contain about 70% water, but the cells that reach the granular layer begin keratinization by the action of keratohyalin and the fluid in the cells escapes and the cells are flat. It will contain about% moisture. The nucleus, cytoplasm and organelles of the cell are degenerated, replaced with keratin-hyline and then converted back to eleidin, which is the final stage of keratin, and the life of the cell is pushed up to the stratum corneum.

When keratinization reaches the stratum corneum, moisture is reduced to less than 20%, turning into keratinocytes. When it contains about 20% moisture, the skin is moist, firm, and shiny to maintain a beautiful and fair skin condition. However, when the skin's moisture drops below 15%, it becomes dry, cracked, and rough skin.

The flattened keratinocytes are 15 to 40 rows of layers that are like bricks, and the layers are firmly touching the outer skin. Aged cells are made of a substance called intercellular cement that acts as an adhesive to prevent the cells from falling apart. Does not deviate and sticks to the skin surface. Cells adhered to the intercellular cement will not fall off quickly as the cell ages and the circulation of keratinocytes will not occur, making the skin dull and rough as well as aging, causing wrinkles and rough skin.

Keratin is a sulfur-containing protein. Skin is soft keratin with 20% moisture. Hair and nails are hard keratin without moisture. Dead cells that are keratinized continue to fall off the skin surface. When women enter middle age, the cells lose their vitality by prolonged cell turnover cycles due to poor cell detachment or desquamation for various reasons. Peeling products to prevent this and artificially promote desquamation or abortion, peeling the epidermis by various methods such as dermabrasion and chemical exfoliation to stimulate cell division and proliferation in the basal layer so that young and fresh cells are produced. This is used.

The cell regeneration cycle refers to a life cycle of cells, ie, one rotation cycle of cells, until renal cells made by cell division in the basal layer grow and reach and fall off to the stratum corneum. Transit time is the transit time from the base layer to the stratum corneum. The run time from the base layer to the granule layer is 14 days, and it takes 14 days to reach the stratum corneum from the granule layer to reach the stratum corneum and total 28 days. Is said to take. The run time is shortest until the age of 25, so the cell regeneration of the cells is the most active, and since then, it starts to slow down, and it takes twice as long as it reaches 60 years.

According to one survey on the form of keratin protection, 85% of the subjects had concerns about keratin on the face, and 56% of the subjects reported that they had keratin protection. Regarding the necessity of keratin care, 79% of the 46 people said that they needed it, and the reason is that they have dirty and dull skin that does not eat makeup well.

Looking at the method of keratin care so far, there is a method of using a towel using a steam towel, a natural material using a natural material and a method of using a functional product. However, in case of packs, it is inconvenient to wash several times, and in case of alpha hydroxy acid cosmetics, scrub-containing cosmetics and mud packs, skin irritation occurs due to irritation, and in case of creams, keratin There is a problem that the effect of care is insignificant.

Therefore, in order to overcome the problems of the product, Korean Patent Laid-Open Publication No. 10-2001-0100228 discloses keratin among various natural substances in order to effectively destroy a protein called desmosome, an intercellular adhesion material that holds unnecessary keratinocytes. As a result of studying effective substances for removal, bromelain, a proteolytic enzyme obtained from pineapple stems, and papain, a proteolytic enzyme obtained from papaya trees, have excellent proteolytic effects while It is disclosed that there is no problem in stimulation.

However, although the above natural proteolytic enzymes have excellent proteolytic efficacy, the present invention does not consider the sustainability and stabilization of the efficacy problem when the enzymes are actually used as a skin cleanser, cosmetics and the like. In other words, when the protease is mixed with water and the like in the composition, the protease is active and the activity of the enzyme is rapidly decreased in the long term (C & T vol. 111, pp 79-88, June 1996). Even if the enzyme itself shows excellent protein degradation effect, when used in the form of a composition such as a real cosmetics, the effect is dramatically reduced, there is a problem that the effect of exfoliation is insignificant.

Accordingly, a first object of the present invention is to provide a composition for removing skin keratin, in which proteolytic enzymes are prevented from deteriorating in activity due to water, heat, or the like, and the proteolytic effect such as keratin is stabilized.

A second object of the present invention is to provide a method for preparing the skin exfoliation composition.

It is a third object of the present invention to provide a cleansing composition comprising the composition for removing skin exfoliation.

In order to achieve the first object of the present invention, the present invention is about 40 to 50 parts by weight of the seed layer, 0.00001 to 10.0 parts by weight of the enzyme layer formed by coating a proteolytic enzyme on the seed layer, and the enzyme layer It provides a coating composition for exfoliating skin comprising 0.5 to 10.0 parts by weight of a stabilizer layer formed by coating a betaine.

Here, cellulose, starch, lactose, talc, etc. may be used as the seed layer, bromelain or papain may be used as the protease, and cocamidopropyl betaine may be used as the betaining agent. (cocamidopropyl betaine) or 2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazolinium betaine (2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazolinium betaine), etc. can be illustrated. In addition, preferably a wax layer formed by coating about 0.5 to 3 parts by weight of wax on the seed layer and an additive layer formed by coating an additive on the wax layer, the enzyme layer is formed by coating on the additive layer, It may further include a surfactant layer formed by coating a surfactant on the stabilizer layer. At this time, the additives include 1 to 3 parts by weight of oligomeric proanthoCyanidins (OPC), 0.1 to 3.0 parts by weight of aloe, and 0.5 to 1.5 parts by weight of licorice extract.

In order to achieve the second object of the present invention described above, the present invention comprises the steps of dissolving wax in ethanol and coating a cellulose to form a coating seed layer, by coating a proteolytic enzyme on the coating seed layer to form an enzyme layer It provides a method for producing a skin exfoliation composition comprising the step, and forming a stabilizer layer by coating a betaine on the enzyme layer.

In the above-described manufacturing method, preferably, further comprising the step of forming an additive layer by coating an additive on the coating seed layer, the enzyme layer is formed by coating on the additive layer, a surfactant on the stabilizer layer Coating to form a surfactant layer and mixing a binder with a stabilized enzyme including the coating seed layer, the additive layer, the enzyme layer, the stabilizer layer, and the surfactant layer to form granules of 100 to 180 μm. It may further include. In addition, in the above-described manufacturing method, the coating may be prevented from being degraded by contacting water with an enzyme by performing multiple coating by a fluid bed process.

In order to achieve the above-mentioned third object of the present invention, the present invention provides a skin cleanser comprising about 0.0001 to 10 parts by weight of the exfoliating composition, about 30 to 50 parts by weight of surfactant, and about 40 to 50 parts by weight of cellulose. To provide a composition.

According to the present invention, a protein exfoliation composition excellent in exfoliation effect in which the proteolytic effect of the enzyme is kept constant regardless of external influences such as moisture or heat can be prepared, and the unnecessary exfoliation component of the outermost layer of the skin It is possible to provide a skin cleansing composition capable of effectively removing impurities present in pores and the like on the skin surface.

 Hereinafter, the present invention will be described in more detail.

The skin exfoliation composition of the present invention includes a seed layer. The seed layer may be cellulose, starch, lactose, talc, etc., and about 40 to 50 parts by weight of the total coating composition is used. The center of the granular protease coating composition formed by coating the proteolytic enzymes described below is formed. Located in As an application example, a protease described later may be applied onto a nonwoven fabric by using a nonwoven fabric instead of the seed layer.

The skin exfoliation composition of the present invention comprises an enzyme layer formed by coating a proteolytic enzyme on the seed layer. The proteolytic enzyme may be used alone or mixed about 0.00001 to 10.0 parts by weight of bromelain or papain. If it is used less than 0.00001 parts by weight, it is difficult to expect a sufficient exfoliation effect, when used in excess of 10.0 parts by weight causes a skin irritation.

Bromelain is a protease obtained from the pineapple stem, and papain is a protease obtained from the papaya tree. It has an excellent effect of removing impurities on the skin surface such as pores and pores. In addition, the protease is a natural substance that has the effect of preventing the opening of the skin, skin damage due to skin irritation generated when removing keratin and the like through existing chemicals. 1A and 1B are photographs showing the skin condition before using the protease, and FIGS. 2A and 2B are photographs after using proteases for the skin of FIGS. 1A and 1B, respectively.

Comparing FIG. 1A with FIG. 2A, it can be clearly seen that the skin condition after the use of the protease is clearly improved, and the same conclusion can be obtained by comparing FIGS. 1B and 2B.

In addition, the protease coating composition of the present invention includes a stabilizer layer formed by coating a betaine on the enzyme layer.

The betaine which may be used as the stabilizer layer is cocamidopropyl betaine or 2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazolinium betaine (2-alkyl-N-carboxymethyl- N-hydroxyethyl imidazolinium betaine) and the like, and about 0.5 to 10.0 parts by weight is used. If the amount is less than 0.5 parts by weight, the role of the enzyme as a stabilizer is insignificant. If the amount is more than 10.0 parts by weight, the fluidity of the formulation is sharply dropped due to the physical properties of the betaining agent, the products are entangled with each other.

The betaine-based material plays a very important role in stabilizing the enzyme. The enzymes such as bromelain or papain are excellent in proteolytic effect, but when exposed to water or heat using a cleansing composition or the like, there is a problem in that the proteolytic effect is rapidly lowered. Figure 3 compares the proteolytic effect when using the bromelain itself and when the enzyme is coated with a stabilizing material such as betaine as in the present invention.

Referring to Figure 3, the proteolytic enzyme (A) in the multilayer membrane coating structure shows an activity difference of more than 70% in the activity of the enzyme compared to the unbroken pure bromelain enzyme (B). Enzyme particles stabilized with such a multilayer membrane contribute to enhancing the cleaning effect in the skin cleansing composition of the present invention using the same.

In addition, the skin exfoliation composition according to the present invention may preferably further include a wax layer, an additive layer, and a surfactant layer.

The wax layer is coated on the seed layer made of cellulose and the like, and the wax is dissolved in ethanol and coated. The wax layer serves to protect the skin by forming a thin film on the skin together with the additive layer upon cleansing. It is preferable to use about 0.5 to 3 parts by weight of the wax layer, but when using less than 0.5 parts by weight, there is a problem that the formation of a skin protective film is insignificant. have.

The additive layer is formed by coating on the wax layer. Representative materials that can be used are OPC, aloe, licorice extract, etc., which are mixed or used alone. The OPC extract is an antioxidant component that smoothes the metabolism of the skin and is effective in moisturizing and forming a combined structure of collagen, and aloe and licorice extract are natural ingredients such as skin moisturizing and soothing.

When mixed with the additive materials, it is preferable to include about 1 to 3 parts by weight of OPC extract, about 0.1 to 3.0 parts by weight of aloe, and about 0.5 to 1.5 parts by weight of licorice extract. If it is used less than the above-mentioned amount is not easy to achieve the intended effect of the material, when used in excess, there is a problem from an economic point of view.

In addition, the surfactant layer is formed by coating a surfactant on the stabilization layer. As the surfactant, sodium (Na) N-acryl-L-glutamate or sodium (Na) tetradecenesulfonate may be used alone or in combination. 40 parts by weight is used.

The surfactant component plays a role of increasing the cleaning power and foaming of the cleansing composition, when using less than 20 parts by weight can not expect such an effect, when used in excess of 40 parts by weight is less economical.

Method for producing a protease coating composition of the present invention comprises the step of dissolving the wax in ethanol to coat the seed to form a coating seed layer. The cellulose, starch, lactose and talc as seeds are used to coat the wax dissolved in ethanol on the cellulose using a fluidized bed process apparatus or the protease is applied onto the nonwoven fabric with the nonwoven fabric as a seed.

Thereafter, the proteolytic enzyme is coated on the prepared coating seed layer to form an enzyme layer. As the protease, bromelain or papain may be used alone or in combination as described above.

Finally, by coating a betaine on the enzyme layer to form a stabilizer layer to prepare a protease coating composition. The betaine which may be used as the stabilizer layer is cocamidopropyl betaine or 2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazolinium betaine (2-alkyl-N-carboxymethyl- N-hydroxyethyl imidazolinium betaine) etc. can be illustrated.

 In the above manufacturing process, the coating is performed by a fluid bed process, which is one of various encapsulation methods that proteolytic enzymes perform to prevent the activity from falling under the influence of heat or moisture in the material. It is preferable to use a fluid bed granulation coater (Fluid Bed Coater; manufactured by Glatt, Germany).

In addition, preferably, the method may further include forming an additive layer by coating an additive on the coating seed layer in the method for preparing a protease coating composition. As the additive, OPC, aloe, licorice extract, etc. may be mixed or used alone, thereby facilitating the metabolism of the skin and having an effect on moisturizing and forming a collagen structure.

The method may further include forming a surfactant layer by coating a surfactant on the stabilizer layer and mixing a binder with a stabilized enzyme formed up to the surfactant layer to form granules of 100 to 180 μm. As the surfactant, sodium (Na) N-acryl-L-glutamate or sodium (Na) tetradecenesulfonate may be used alone or in combination.

The skin exfoliating composition for exfoliating the skin of the present invention includes the composition for exfoliating the skin and other substances for preparing the cleansing composition. Specifically, about 0.0001 to 10 parts by weight of the protease coating composition, about 30 to 50 parts by weight of surfactant, and about 40 to 50 parts by weight of cellulose. The surfactant may be sodium lauroyl glutamate or sodium cocoyl glutamate, and the like, which has an effect of imparting cleaning power.

The face wash composition may be specifically prepared in the form of a dry powder or a nonwoven fabric. Specifically, in the case of manufacturing the nonwoven fabric, the bromelain or papain solution is applied on the nonwoven fabric and dried, and the betaine is applied and then dried to achieve the same effect as the coated protein enzyme. The additives required for the cleaning composition may be further applied to prepare the cleansing composition.

The cleansing composition can solve the existing processes such as cleanser, soap cleansing, and skin moisturizing at once, and because it does not use a surfactant that is highly irritating to the skin, it is a weakly acidic cleansing agent of pH 5.5-6.5 similar to the skin pH. There is no burden.

Hereinafter, the present invention will be described according to preferred embodiments of the present invention.

Example 1

In order to determine the degree of skin irritation of the skin cleansing composition of the present invention described above, skin irritation experiments were conducted using the US FDA's Shelanski Shelanski procedure.

Specifically, a patch unit was used as a fin chamber, and the test site was wiped with 70% ethanol with the forearm and dried, and then 20 µl of the skin cleansing composition was added dropwise into the chamber to obtain a similar skin condition. The test subjects were applied to 20 subjects and the application site was marked with a marking pen. After 24, 48, 72 hours closed patch, after removing the patch skin reaction was evaluated in the ICDRG (International Contact Dermatitis Research Group) regulations and the results are shown in Table 2. The symbols according to the evaluation results and their meanings are shown in Table 1.

sign Judgment contents - voice ± Suspicious or slight erythema, etc. + Weak reactions (without vesicles), erythema, papules ++ Neutral reactions (with vesicles), erythema, papules, vesicles +++ Strong reaction, cannon reaction

         Elapsed time subject 24 hours 48 hours 72 hours Subject 1 - - - Subject 2 - - - Subject 3 - - - Subject 4 - - - Subject 5 - - - Subject 6 - - - Subject 7 - - - Subject 8 - - - Subject 9 ± - - Subject 10 - - - Subject 11 - - - Subject 12 - ± - Subject 13 - - - Subject 14 - - - Subject 15 - - - Subject 16 - - - Subject 17 - - - Subject 18 - - - Subject 19 - - - Subject 20 - - -

As a result of the above experiment, as shown in [Table 2], the skin cleansing composition of the present invention was found to effectively exfoliate keratin and clean the skin without showing any irritation to the skin.

Example 2-4

A skin cleansing composition comprising a composition for removing skin exfoliation stabilized by the manufacturing method of the present invention was prepared in a dry powder form with the composition shown in the following [Table 3]. Enzymes were used for the stabilized bromelain coating composition according to the present invention for Examples 2-4. Amisoft LS-11 and Amisoft CS-11 were the product names of the surfactants sodium lauroyl glutamate and sodium cocoyl glutamate, respectively. After the composition thus prepared was left at 40 ° C. for 30 days, the activity of the protease was measured and shown in Table 3 below.

[Comparative Example]

In the same manner as in Examples 2 to 4 to prepare a dry powder composition for skin cleanser, the enzyme was prepared dry powder using a bromelline uncoated.

Example 2 Example 3 Example 4 Comparative example  enzyme  10.0 10.0 10.0 10.0  Amisoft LS-11  20.0  20.0  20.0  20.0  Amisoft CS-11  15.0  15.0  15.0  15.0 SiO ₂  1.0  1.0  1.0  1.0  Betaine 3.0 5.0 10.0 3.0  cellulose 50.0 48.0 43.0 60.0 Enzyme activity 93% 95% 95% 23%

Referring to [Table 3] showing the enzyme activities of Examples 2 to 4 and Comparative Examples, in Examples 2 to 4 using the coating composition of the present invention in the activity, the activity of the enzyme even after 30 days at 40 ℃ While it was maintained at 93% or more, it was observed that the activity of the enzyme rapidly decreased in the comparative example. That is, even in the case of adding the diarrhea betaine in the comparative example it was confirmed that the effect of enzyme stabilization simply by mixing in a form that is not directly coated on the enzyme.

 Example 5

A facial cleansing composition in which an active ingredient was adsorbed on a non-woven fabric of a powder-type cleansing composition of Examples 2 to 4 was prepared. Specifically, the uncoated bromelain was dissolved in water and sprayed onto the nonwoven fabric through a spray method within 10 minutes, followed by instant drying, followed by spraying betaine to the next step to dry. Thereafter, the surfactant liquid cleansing composition giving the cleaning power was finally adsorbed onto the nonwoven fabric to prepare a nonwoven fabric having the enzyme adsorbed thereto.

The dried betaine layer of the nonwoven fabric prepared by the above method acted as a stabilizer layer to keep the activity of the enzyme constant. In the case of manufacturing the non-woven fabric form, it is possible to effectively remove the dead skin surface by effectively washing the skin by using the continuous enzyme activity and to overcome the disadvantages such as dry powder type dusting.

According to the present invention, a protein exfoliation composition excellent in exfoliation effect in which the proteolytic effect of the enzyme is kept constant regardless of external influences such as moisture or heat can be prepared, and the unnecessary exfoliation component of the outermost layer of the skin It is possible to provide a skin cleansing composition capable of effectively removing impurities present in pores and the like on the skin surface.

Although described above with reference to a preferred embodiment of the present invention, those skilled in the art will be variously modified and changed within the scope of the invention without departing from the spirit and scope of the invention described in the claims below I can understand that you can.

1A and 1B are photographs showing the skin condition before using protease.

Figures 2a and 2b is a photograph after the use of protease for each of the skin of Figures 1a and 1b.

Figure 3 is a graph showing the change in activity (protein degradation efficiency) with time of bromelain enzyme.

Claims (13)

a) 40 to 50 parts by weight of a seed layer, characterized in that one selected from the group consisting of cellulose, starch, lactose, talc, and nonwoven fabrics; b) 0.00001 to 10.0 parts by weight of an enzyme layer formed by coating a proteolytic enzyme characterized by bromelain or papain on the seed layer; And c) Cocamidopropyl betaine or 2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazolinium betaine (2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazolinium betaine) on the enzyme layer 0.5 to 10.0 parts by weight of a stabilizer layer formed by coating a betaine, characterized in that one selected from the group consisting of Skin exfoliation composition comprising a delete delete delete According to claim 1, Wax layer formed by coating 0.5 to 3 parts by weight of wax on the seed layer; An additive layer formed by coating an additive which is a mixture including 1 to 3 parts by weight of oligomeric ProanthoCyanidins, 0.1 to 3.0 parts by weight of aloe, and 0.5 to 1.5 parts by weight of licorice extract on the wax layer; And The enzyme layer is formed by coating on the additive layer, the surfactant layer formed by coating 20 to 40 parts by weight of the surfactant on the stabilizer layer Skin exfoliation composition, characterized in that it further comprises. delete Dissolving the wax in ethanol and coating the seed to form a coating seed layer; Forming an enzyme layer by coating a proteolytic enzyme, wherein the proteolytic enzyme is bromelain or papain on the coating seed layer; And Cocamidopropyl betaine or 2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazolinium betaine (2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazolinium betaine) on the enzyme layer Coating a betaine, characterized in that to form a stabilizer layer Method for producing a skin exfoliation composition comprising a. 8. The method of claim 7, wherein the coating is carried out by a fluid bed process. delete delete 8. The method of claim 7, further comprising: forming an additive layer by coating an additive on the coating seed layer, wherein the additive is at least one selected from the group consisting of oligomeric proanthocyanidins, aloe, and licorice extract; The enzyme layer is formed by coating on the additive layer, and forming a surfactant layer by coating a surfactant on the stabilizer layer; And Of the composition for removing skin exfoliation further comprising forming a granule of 100 to 180 ㎛ by mixing a binder with the stabilized enzyme comprising the coating seed layer, the additive layer, the enzyme layer, the stabilizer layer, and the surfactant layer. Manufacturing method. delete a) 40 to 50 parts by weight of a seed layer, characterized in that one selected from the group consisting of cellulose, starch, lactose, talc, and nonwoven fabrics; 0.00001 to 10.0 parts by weight of an enzyme layer formed by coating a proteolytic enzyme, characterized in that bromine or papain on the seed layer; And Cocamidopropyl betaine or 2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazolinium betaine (2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazolinium betaine) 0.0001 to 10 parts by weight of the composition for removing skin exfoliation comprising 0.5 to 10.0 parts by weight of a stabilizer layer formed by coating a betaine, characterized in that the one selected from the group consisting of b) 30 to 50 parts by weight of the surfactant; And c) skin cleansing composition comprising 40 to 50 parts by weight of cellulose.