JP2009249288A - Detergent composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a detergent composition pursuing basic functions required as a detergent in high levels, especially decomposing and removing the sebum of skin in a good efficiency and improved in skin irritation on its use. <P>SOLUTION: This detergent composition contains a lipase as a fat decomposing enzyme, at least one extract selected from the extract of neem (Melia azadirachta) and the extract of lemon grass (Cymbopogon citratus), and further contains a protease as a protein decomposing enzyme in the above components. The preservation form of the detergent composition is a powdery or granular form, or a portion type, and especially it is a face-washing detergent composition. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a cleaning composition, and more particularly to a cleaning composition excellent in efficiently decomposing and removing sebum from the skin.

The cleaning composition used to clean the face of the human body mainly maintains the health of the skin by removing dirt from the sebaceous waste on the skin surface, dirt from pores accompanying it, and the old stratum corneum that accompanies skin metabolism. This is very important for maintaining the cleanliness of the surface. For this reason, the formulation which mix | blended the lipolytic enzyme is already known (patent document 1, 2).
Further, a method for producing a neem extract having low skin irritation by solvent extraction from neem steam distillation residue and a hypoallergenic cosmetic composition containing this neem extract are known (Patent Documents). 3).
Moreover, it is known that a lemongrass extract has a lipase activity promotion effect (patent document 4).
However, as a cleansing composition for human face washing, sebum is decomposed and removed to improve the darkening of pores, and the amount of sebum degrading enzyme used is reduced to reduce irritation to the skin during use derived from the enzyme. There are no known cleaning compositions that can be used.
Japanese Patent Laid-Open No. 02-150498 Japanese Patent Laid-Open No. 06-217771 JP 2006-76909 A JP 2004-238310 A

  The present invention is a novel cleaning composition that pursues a high level of basic functions required as a cleaning composition in view of the prior art, and in particular, a cleaning composition suitable for face washing that can reduce irritation to the skin. The purpose is to provide goods.

The present inventors have intensively studied on a cleaning composition excellent in efficiently decomposing and removing skin sebum. As a result, by adding the neem extract and / or lemongrass extract, which are natural ingredients, to the lipolytic enzyme, the activation of the lipolytic enzyme is promoted and the sebum is decomposed and removed to improve the darkening of pores. As well as being able to reduce the amount of enzyme used, it was possible to reduce irritation to the skin during use due to the enzyme. The present invention has been made based on such findings.
That is, the cleaning composition of the present invention includes a lipolytic enzyme and at least one extract selected from a neem extract and a lemongrass extract. In particular, the lipolytic enzyme is a lipase.
In addition, the cleaning composition of the present invention is characterized by further containing a proteolytic enzyme in addition to the above components. In particular, the proteolytic enzyme is a protease.
The cleaning composition of the present invention is characterized in that the storage form of the cleaning composition is powder or granule or is a portion type.
In addition, the cleaning composition of the present invention is for face washing.

  Since the neem extract and / or lemongrass extract, which are natural components, are blended with the lipolytic enzyme, the activity of the lipolytic enzyme is improved. As a result, it removes excessive amounts of fat and dirt on the skin surface of the face, that is, pore dirt, effectively removes dead skin, and restores and promotes skin metabolism. Gives the skin a luster and luster, making it possible to obtain a skin-whitening skin with no dullness and transparency. In addition, the cleaning composition of the present invention provides an ideal cleaning composition that is substantially free of skin irritation.

The lipolytic enzyme that can be used in the cleaning composition of the present invention is formulated for the purpose of removing excessive amounts of lipids on the skin surface and effectively removing dirt on pores. Any material that satisfies this purpose and can be blended into cosmetics can be used. Specifically, for example, lipases derived from microorganisms and yeasts belonging to the genus Rhizopus, which are in a form that can be blended in cosmetics, can be exemplified.
The amount of the lipolytic enzyme varies depending on the form of the cleaning composition of the present invention and the form of use, but is usually 0.01 units or more, preferably 0.1 to 100, per gram of the cleaning composition in terms of solid content. It is desirable to blend 000 units, more preferably 1 to 10,000 units, and even more preferably 10 to 1,000 units. Here, “unit” represents “enzyme unit”, and represents an enzyme amount (1 μmol / min) that can change a substrate of 1 micromole (μmol) per minute at a temperature of 30 ° C. with the acidity at which the chemical reaction proceeds most. To express.

The neem extract that can be used in the present invention is an extract of a neem plant (Melia azadirachta L. (= Azadirachta indica Juss.)). Nimes are also known as neem, or Margosa. Azadirachta excelsa, which is a plant of the Sendan family, can also be used in combination.
Neem leaves, flowers, flower spikes, pericarp, fruit seeds, branches and leaves, bark, rhizome, root bark, roots, seeds, above-ground parts or whole plants can be used as extraction raw materials. Among these, leaves containing branch leaves are particularly preferable. Neem leaves are preferably extracted after being collected and then subjected to pretreatment such as drying and cutting.

The extraction solvent for obtaining the neem extract can be selected in consideration of the intended purpose and type of the product to be provided, or the subsequent processing. Usually, water, methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, tert-butanol and other alcohols, hexane, pentane, diethyl ether, chloroform, dichloromethane, acetone, acetonitrile, ethyl acetate 1 type, or 2 or more types of liquid mixture chosen from among these can be used. It is preferable to use water.
Regarding the extraction method, the temperature of the solvent, the weight ratio of the solvent to the raw material, or the extraction time can be arbitrarily set for various raw materials and the solvent used. Further, the weight ratio of the solvent to the raw material can be arbitrarily set, for example, within the range of the dry raw material: solvent ratio of 1: 1 to 1: 500, and the weight ratio of 1: 5 to 1:50 is particularly preferable. .

“Lemongrass” (also known as incense, lemon sow, lemon gaya) that can be used in the present invention is a plant belonging to the Gramineae and Cymbopogon genus: Lemongrass “Cymbopogon citratus (A.DC.) STAPF (= Andropogon citratus A.DC. = A.schoenanthus LINN. = A.roxburghii NEES = A.ceriferus HACK. = A.nardus LINN. = Schoenanthumamboinicum RUMPH.) Branches, leaves, stems, bark, rhizomes, root barks, roots, seeds or whole plants are used, but other species of the same genus (C.conferflorus STAPF), (C.giganteus), Koiusigaiya (aka: Citronella) (C. nardus) ), (C. pendulus NEES EX STEUD. W. WATSON), Ogarkaya (C. tortilis = Andropogon. C amus), Java citronella (C. winterianus), etc. can be used.
Various parts of the above plant body (flowers, flower spikes, fruit spikes, pericarp, fruits, stems, leaves, branches, branches and leaves, trunks, bark, roots, rhizomes, root barks, seeds or whole plants) as they are or after pulverization, with a solvent Extracted.

  As an extraction solvent for the lemongrass extract, water, lower alcohol such as methanol, ethanol, propyl alcohol, isopropyl alcohol, butanol, isobutanol, pentanediol or the like, hydrous lower alcohol, propylene glycol, 1,3-butylene glycol, glycerin, etc. It is desirable to use one kind or a mixture of two or more kinds selected from various organic solvents such as polyhydric alcohols or hydrous polyhydric alcohols, acetone, ethyl acetate and the like. However, if it is not preferable to contain an organic solvent depending on the purpose of use, use only water, or ethanol that is easy to remove after extraction, and use alone or in any mixture with water, or extract by extraction. Or something like that. Furthermore, it can be used in the state where each solvent extract is combined. In addition, the extraction method is not particularly limited, but usually it may be in the range of the boiling point of the solvent at normal temperature and normal pressure, and can be used as it is extracted.

In the cleaning composition of the present invention, the activity of the lipolytic enzyme contained in the cleaning composition of the present invention is improved by blending the extract of neem and / or lemongrass, which are the above plant extracts.
The results of investigating the activation of lipolytic enzyme are shown below.
Activation confirmation test of lipolytic enzyme The verification test of the lipase activity by the plant extract of lipolytic enzyme was conducted.
(1) Test substance Test substance 1: 1 L of purified water was added to 100 g of neem (Melia azadirachta L.) leaves (dried, chopped), extracted for 3 hours in a boiling water bath, cooled, and then made by Toyo Roshi. It filtered using No5C.
Test substance 2: 100 g of whole grass (dried product, chopped) of lemongrass (Cymbopogon citratus) was added with 1 L of purified water, extracted for 3 hours in a boiling water bath, cooled, and then filtered using No5C made by Toyo Roshi. did.

(2) Test method: In this test, lipase activity was measured using a human blood lipase assay reagent (Lipase Kit S: manufactured by Dainippon Pharmaceutical Co., Ltd.). The sample used for the test is a mixed solution of 20% test drug, 25% 0.04% lipase (MY-30 manufactured by Meito Sangyo Co., Ltd.), and 55% purified water, and 8 μL of the sample, 76 μL of color developing solution, and esterase inhibitor 8 μL of 25% solution was mixed and warmed at 30 ± 1 ° C. for 5 minutes. Thereafter, 8 μL of the substrate solution was added and the mixture was shielded from light and incubated at 30 ± 1 ° C. for 30 minutes. After completion of the incubation, 152 μL of a reaction stop solution was added to stop the reaction, and the absorbance at a wavelength of 405 nm was measured.
As a control, a solvent for the sample was used, and a blind was added in which the substrate was added after the reaction was stopped. The relative value of lipase activity was calculated from the difference in absorbance between the sample and the blind by the following formula.

Calculation method: Relative value = [(Sample Absorbance of Test Drug−Blind Absorbance of Test Drug) / (Control Sample Absorbance−Blind Absorbance of Control)] × 100

表１の結果から明らかなように、本発明の洗浄組成物に有効成分として配合される脂肪分解酵素は、ニームの葉抽出エキスおよびレモングラス抽出液によって、対照に比してリパーゼ活性の上昇が認められる。 (3) Results

As is apparent from the results in Table 1, the lipolytic enzyme blended as an active ingredient in the cleaning composition of the present invention has an increase in lipase activity compared to the control by the neem leaf extract and lemongrass extract. Is recognized.

The cleaning composition of the present invention improves lipolytic enzyme activity by blending neem extract alone, lemongrass extract alone, or a mixture of neem extract and lemongrass extract with lipolytic enzyme. To do. The neem extract is preferably used as an essential component, and the activity of the lipolytic enzyme is further improved by adding a lemongrass extract.
The blending amount of the neem extract is usually 0.0001 mg or more, preferably 0.001 to 100 mg, more preferably 0.005 to 10 mg, still more preferably 0.000 mg or more per g of the solid content conversion cleaning composition. It is desirable to blend 01 to 5 mg.
In addition, the lemongrass extract is usually 0.0001 mg or more, preferably 0.001 to 100 mg, more preferably 0.005 to 10 mg, and still more preferably 0.001 mg or more per gram of the solid content conversion cleaning composition. It is desirable to blend 01 to 5 mg.

  The proteolytic enzyme that can be used in the present invention is blended mainly for the purpose of effectively removing keratin of aged skin, and can be used as long as it satisfies this purpose and can be blended into cosmetics. Specific examples include proteases derived from microorganisms such as Bacillus subtilis and actinomycetes, which are in a form that can be blended in cosmetics. In addition, papain can also be used as a protease. The amount of the protease to be mixed varies depending on the form of the cleaning composition of the present invention and the form of use, but is usually 0.01 units or more, preferably 0.1 to 10,000 units per gram of the solid-based cleaning composition. More preferably, it is desirable to blend 0.1 to 1,000 units, and still more preferably 0.1 to 100 units.

The cleaning composition of the present invention can be used as a cleaning composition used for face washing, a cleaning composition used for the body (for whole body), and a cleaning composition used for fingers. Of these, a facial cleansing composition used on the face with the highest amount of sebum is preferred.
The cleansing composition used for facial cleansing contains trehalose as a moisturizing component, raffinose as a moisturizing component, and hydroxypropylcellulose as a foaming aid component and allantoin as an anti-inflammatory component. It is preferable that the lipolytic enzyme and at least one extract selected from neem extract and lemongrass extract are blended, and a proteolytic enzyme is blended if necessary.

  The cleaning composition of the present invention may contain other raw materials as necessary, for example, surfactants (anionic surfactants such as carboxylates, sulfonates, sulfates, phosphates, and amphoteric interfaces such as carboxylates). Activators, polyhydric alcohol aliphatic esters, polyhydric alcohol alkyl ethers, polyoxyethylene ethers, ether esters, non-ionic surfactants such as nitrogen-containing derivatives, fluorine-based surfactants such as perfluoroalkyl phosphate esters, poly Silicone surfactants such as ether-modified silicones, natural surfactants such as polypeptide derivatives and sugar surfactants), water-soluble polymers and polyhydric alcohols, saccharides (polyacrylic acid, polyvinyl alcohol, polyvinylpyrrolidone carboxy) Sodium methylcellulose, methylcellulose, casein, pectin, Pump, sodium alginate, locust bean gum, carrageenan, agar, hydroxyapatite, glycerin, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, Propylene oxide, propylene glycol, polypropylene glycol, 1,3-butylene glycol, pentyl glycol, pentaerythritol, threitol, arabitol, xylitol, ribitol, galactitol, sorbitol, mannitol, lactitol, maltitol), powder, scrub agent ( Nylon powder, silk powder, urea Powder, Teflon (registered trademark) powder, silicone powder, polymethyl methacrylate powder, cellulose powder, silicone elastomer spherical powder, polyethylene powder) various agents (whitening ingredients, anti-inflammatory agents, anti-aging agents, UV protection agents, slimming) Agents, squeeze agents, antioxidants, moisturizers, blood circulation promoters, antibacterial agents, bactericides, desiccants, cooling agents, warming agents, vitamins, amino acids, wound healing promoters, stimulant mitigants, analgesics, Cell activator) and the like can be blended.

  The cleaning composition may be in the form of a solid such as powder or granule, gel, paste, foam, liquid, etc., and also moisture absorption such as cotton, cloth, paper, sponge, nonwoven fabric, etc. The thing of the form which adhered or impregnated the washing | cleaning composition of this invention to the property raw material can be illustrated. Preferred storage forms include solid forms such as powder or granules. The cleaning composition in this storage form can be filled or packaged in a suitable container, and further filled or packaged in a portion-type suitable container as necessary to obtain a final product.

A method for using the cleaning composition of the present invention thus obtained will be described.
Although it varies depending on the amount of the active ingredient contained in the cleaning composition of the present invention, for example, when the cleaning composition is in a solid form such as powder or granules, it is usually 0.01 g or more, preferably 0.1 to 0.1 g. 100 g, more preferably 0.2 to 10 g, more preferably 0.3 to 3 g is dissolved in an appropriate amount of water, warm water or warm water. At this time, when a small amount of the cleaning composition is dissolved in a small amount of water, lukewarm water or hot water, the cleaning composition can be dissolved by the palm of the hand. Next, an aqueous solution containing the cleaning composition dissolved therein is applied to the face skin locally or gently on the entire face skin, and the applied state is maintained for about 0.5 to 5 minutes, preferably about 1 to 3 minutes. Next, the face is carefully rinsed with water, lukewarm water or warm water.

When the cleaning composition of the present invention is solid or liquid such as gel, paste, foam, liquid, etc., usually 0.1 to 5 g, preferably 0.2 to 3 g, more preferably After 0.3 to 1 g is dissolved or suspended as it is or with an appropriate amount of water, lukewarm water or warm water, it is picked up and applied to the face skin gently or evenly on the entire face skin. This state is maintained for about 0.5 to 5 minutes, preferably about 1 to 3 minutes, and then washed with water, lukewarm water or warm water with a gentle rinsing procedure.
In addition, when maintaining the state which applied the said washing | cleaning composition to the facial skin, the effect | action of a proteolytic enzyme and a lipolytic enzyme can be accelerated | stimulated by applying a steam towel etc. to a facial skin lightly. In addition, when the cleaning composition of the present invention is applied locally to the facial skin, it is also optional to apply it locally to areas of concern such as pore dirt, dull skin, spots, freckles and the like. Although there is no restriction | limiting in particular in the frequency | count of face washing per day of the cleaning composition of this invention, Usually, it is effective 1-5 times / day, Preferably it is 1-3 times / day. After washing the face, there is no limit to makeup using, for example, lotion, milky lotion, cream, oil, pack, makeup cosmetics, etc., in accordance with the daily lifestyle of the user.

Examples 1 to 3 and Comparative Example 1
The raw materials shown in Table 2 were weighed and the whole was homogenized three times through a 26 mesh sieve to obtain a powdery product. This powdery product was filled in a packaging container by 0.3 g per package to obtain a powdery cleaning composition. Trehalose is from Hayashibara Biochemical Laboratories, Inc., Raffinose is from Asahi Kasei's oligo GGF, Hydroxypropylmethylcellulose is from Shin-Etsu Chemical's HPMC, Maltose / sucrose condensate is from Nikko Camicals' Bioecoria, Allantoin is Allantoin manufactured by Kawaken Fine Chemical Co., Ltd., lipase manufactured by Meika Sangyo Co., Ltd., trade name “Lipase MY-30” (30,000 units / g), protease manufactured by Amano Enzyme Co., Ltd., trade name “Papain W-40” ”(400,000 units / g), which was previously triturated with trehalose so as to be 1,000 units / g, Photosensitive element 401 was extracted with Neem leaf from Photosensitive element 401 manufactured by Hayashibara Biochemical Laboratories. And lemongrass extract were lyophilized from the extract prepared by the same method as test substances 1 and 2 above Each was used.

The above examples and comparative examples were subjected to the following experiments.
Experiment 1
The oil content of the cheeks of 20 healthy men aged 25 to 55 was measured with an oil meter (Courage SM815 manufactured by Courage + Khazaka electronic GmbH).
Each example or comparative example was dissolved in about 2 mL of water or lukewarm water in the palm of the hand, and this aqueous solution was gently and uniformly applied to the entire skin of the face and kept in the applied state for 2 to 3 minutes. After that, they were carefully washed with water or lukewarm water so as not to leave any rinse.
The oil content was measured with the oil content meter 10 minutes after the face washing was completed.
Table 3 shows the average amount of oil after washing, with the amount of oil before washing as 100.

Experiment 2
16 healthy women aged 21 to 55 and 20 men are divided into 4 groups, and each day, each example or comparative example is dissolved in about 2 mL of water or lukewarm water in the palm of your hand, This aqueous solution was gently and uniformly applied to the entire skin of the face and kept in the applied state for 2 to 3 minutes. Then, the face was carefully washed with water or lukewarm water so as not to leave a rinse.
Other makeup was carried out according to the past methods. This was carried out for 2 months, and they were asked to answer items such as “feeling of freshness of skin”, “feeling of moistness of skin” and “smoothness of skin”. The answer is 3 points for improvement, 2 points for improvement, 1 point for improvement, 0 points for no change (no deterioration), and the total points 4.

As explained above, a cleaning composition containing lipolytic enzyme and neem leaf extract removes excessive amounts of fat and dirt on the skin surface of the face, that is, dirt on the pores, effectively removing old keratin. In addition, the skin metabolism can be recovered and promoted, the skin metabolism can be recovered and promoted, the skin is given luster and gloss, and the skin is whitened with a dull and transparent feeling. The cleaning composition of the present invention is an ideal cleaning composition that is substantially free of irritation to the skin.
Furthermore, the above-mentioned effectiveness became stronger by adding a proteolytic enzyme and an extract of lemongrass.

  Since the cleaning composition of the present invention is an ideal cleaning composition that is substantially free of irritation to the skin, it can be used particularly for facial cleansing used on a face rich in sebum.

Claims (7)

  A cleaning composition comprising a lipolytic enzyme and at least one extract selected from a neem extract and a lemongrass extract.   The cleaning composition according to claim 1, wherein the lipolytic enzyme is a lipase.   The cleaning composition according to claim 1 or 2, further comprising a proteolytic enzyme.   The cleaning composition according to claim 3, wherein the proteolytic enzyme is a protease.   The cleaning composition according to any one of claims 1 to 4, wherein a storage form of the cleaning composition is a powder or a granule.   The cleaning composition according to any one of claims 1 to 5, wherein a storage form of the cleaning composition is a portion type.   The cleaning composition according to any one of claims 1 to 6, wherein the cleaning composition is for face washing.