KR20120040172A - Black doraji extract and its manufacturing method - Google Patents

Abstract

PURPOSE: A black bellflower root extract, and a producing method thereof are provided to offer functionality of the black bellflower roots obtained by steaming fresh bellflower roots to users. CONSTITUTION: A producing method of a black bellflower root extract comprises the following steps: steaming washed bellflower roots at 60 deg C for 15 days; drying the steamed bellflower roots at 30 deg C for 3 hours to obtain black bellflower roots; mixing 40g of black bellflower roots with 400ml of solvent, and uniformly mixing; obtaining a hot water extract of the mixture by extracting the mixture for 5 hours in a shaking water bath at 70 deg C; decompress-concentrating the obtained extract after filtering with filter paper; and freeze-drying the concentrated extract.

Description

Black doraji extract and its manufacturing method

The present invention relates to a black bellflower extract and a method for manufacturing the same, and more particularly, to provide a black bellflower extract excellent in utility as a health functional food material using a black bellflower obtained by steaming raw bellflower.

As the well-being culture settled along with the change in social structure, interest in health functional foods and medicines derived from natural products increased as well.

Among them, efforts to develop health functional foods using bellflower are continuously increasing.

Bellflower has been widely used for food and medicinal for a long time. Its perennial herb is a perennial herb, and its root, Gilgyeong, is also called Dorat, Gilgyeongchae, and Paekak.

Since it is possible to grow wild by itself and supplied as cultivated production, it is required to develop various products to revitalize domestic bellflower cultivation (Korea Food Science Society, 2004).

Bellflower is an alkaline food rich in fiber and high in calcium and iron (Chung et al., 1997), and imported in large quantities in the form of a living body (Statistics Bureau, 2001). Et al., 2001).

The main pharmacological component is triterpenoid saponin, which is known to have pharmacological effects such as expectorant, antitussive, antipyretic and analgesic, along with placodin, which is a component of arine flavor of bellflower (Korean Food Science Society, 2008).

As the bellflower contains various functionalities and various bioactive ingredients related to it, the possibility of development as a health functional food material is increasing. Accordingly, the use of bellflower is increasing, and research on the bellflower has been actively conducted.

Studies on bellflowers known to date include the study of common components (Chung et al., 1997), pharmacological components (Chung et al., 1997), bronchial efficacy (Lee et al., 2000) and the chemical composition and physiological activity of Jangsaeng bellflower (Shon et al., 2001). And research on the development of dietary supplements (Sung-Hwan Roh, 2007), anti-obesity effects of saponins extracted from bellflower (Han et al., 2002) and saponin analysis of bellflower using HPLC (Kim et al., 2007). Recently, the production and quality characteristics of red bellflower extract, which is a processed product of bellflower, have been studied using steaming.

Steaming refers to steaming and cooking in one shot, and there is a gugupo that means steaming nine times and drying nine times. Steaming is the most commonly used method of making red ginseng, including red ginseng and black ginseng, which are representative processed products using steaming. Black garlic and black deodeok are being developed and marketed as the area is expanded.

In case of steaming ginseng similar to bellflower, brown substance formed during steaming process has been proved to be effective as antioxidant and functional ingredient as well as to improve flavor and color (Lee et al., 1995).

However, studies on the quality characteristics and physiological activities of black bellflower have been insufficient, and systematic and scientific studies on the components and pharmacological actions are still insufficient.

KR Patent Publication 10-2011-0075593

Therefore, the present invention is to produce a black bellflower by steaming from raw bellflower, and then to investigate the quality characteristics and functional components of the black bellflower according to the extraction solvent to provide a black bellflower extract excellent in utility as a health functional food material and a method for producing the same It is.

The present invention is the process of steaming the washed bellflower at 60 ℃ in a thermostat for 15 days,

The process of obtaining a black bellflower by drying the bellflower after the steaming process for 3 hours at 30 ℃ using a dry oven,

400 ml of solvent (tertiary distilled water) per 40 g of the dried black bellflower after the drying process and uniformly mixed to obtain a hot water extract,

The hot water extract is extracted for 5 hours in a shaking water bath of 70 ℃ (shaking water bath),

The extract obtained by repeating the extraction process twice is filtered and filtered under filter paper (filter paper) and concentrated under reduced pressure;

The concentrated extract under reduced pressure is characterized in that it provides a black bellflower extract manufacturing method characterized in that the freeze-dried, and the black bellflower extract prepared by the manufacturing method.

The black bellflower extract prepared by the method for producing black bellflower provided by the present invention, including two general bacteria, exhibits very high antibacterial activity even in three kinds of bronchitis-associated microorganisms, as well as a variety of nutrients, including a high saponin content. It provides a high antioxidant activity, improves the food value of the black bellflower, and promotes the use as a functional food, the expected effect is a great invention.

1 is a real picture showing a common bellflower and black bellflower
Figure 2 is a block diagram showing a black bellflower extract manufacturing method provided by the present invention
3 is a result table of the general ingredients of black bellflower and raw bellflower
4 to 7 shows the antimicrobial activity of each extract of bellflower
8 is a graph showing the effect of black bellflower extract on cell viability in bacterial LPS-stimulated raw material 264.7 macrophages
9 is a graph showing the inhibition rate of black bellflower extract against nitrogen oxides

The black bellflower extract provided by the present invention and a method for producing the same will be described in detail below.

Bellflower is native to East Asia, widely distributed in Korea, Japan, and China, and is native to Korea.

The common bellflower, which we usually refer to, is the root of the bellflower (Kim, In-Rak, 2007), and the shelled bellflower has been soaked in water to remove bitterness and then dried and used for food.

Gilkyung has been widely used not only as a food but also as a useful herbal medicine.It is used in various prescriptions as a herbal herbal medicine. For example, Yongkyongsan Co., Ltd. of Japan is selling bronchial inflammation medicines mainly containing bellflower powder (Noh Sung-hwan, 2007).

In addition, the major pharmacologically active components reported are terpenoid saponins. The pharmacological effects of anti-inflammatory, central inhibitory, blood pressure-lowering, anticholine efficacy, and hemolytic effects have been reported.

As part of the method of increasing the use of bellflower, black bellflower was devised, which was produced through the method of steaming (Chae, 2010). Steaming refers to steaming and ripening in a single room, a method of ripening by providing a moist air at a constant temperature, a method that has been widely used to prepare red ginseng.

Products manufactured and marketed in this manner include red ginseng, black ginseng, black garlic and black onions.

Recently, however, it has been known that benzo (a) pyrene, a carcinogen, is produced from red ginseng and black ginseng prepared by the steaming method. Benzo (a) pyrene was produced by varying the drying temperature. As a result, benzo (a) pyrene was produced the most in the case of augmented vesicles, a method commonly manufactured in Korea. Increased with positive correlation.

The increase in benzo (a) pyrene was confirmed by carbonization of the rice ginseng portion, and the safest steaming temperature of red ginseng and black ginseng was 80 ~ 120 ℃ and drying temperature was below 50 ℃ (Jo et al., 2009).

On the other hand, antioxidant means simply to prevent the action of oxidation. Oxygen is necessary for humans to maintain life, and oxygen that enters the body burns carbohydrates and fats in foods ingested in cells to make energy, and free radicals, that is, free radicals, are formed during oxidation. Also called reactive oxygen species (ROS). ROS are produced mainly in the mitochondria inside cells, and the generated ROS attack cells and cause the loss of original cell function. In addition, by oxidizing genes, the cell's regenerative capacity is lowered, and the signal transduction system in the human body is damaged and immune system is lowered. On the other hand, the human body uses some free radicals to act as a defense by preserving immune cells such as leukocytes and external invading bacteria, and hydrogen peroxide (H ₂ O ₂ ) among free radicals also plays a role as a signaling material. . However, most ROS are harmful to the human body, and the body tries to remove free radicals on its own, but accumulates in small amounts and causes long-term adverse effects such as inducing and promoting aging (Swaver 1981, Fridorich 1986).

Studies on the antioxidant activity of bellflower have been shown to donate electrons to the free radicals to inhibit the aging of the human body and to inhibit the oxidation of fat in food. In addition, the study of electron donating ability, which is used as a measure of the action of inhibiting aging by active radicals in the human body, reported that the ethanol extract of 4 years old bellflower showed 48.7% of EC ₅₀ near 90 ppm concentration. The scavenging activity showed 50.5% scavenging activity at the concentration of 9 mg / mL in the four year old bellflower (Shon et al., 2001).

In addition, other studies evaluated antioxidant effects on peroxyl radical and peroxynitrite by TOSC assay, which showed about 0.57 times the effect of GSH used as a positive control in the saponin fraction of bellflower. It was reported that the effect was about 0.12 fold (Kim et al., 2010).

Total antioxidant activity studies showed that the antioxidant activity of bellflower was somewhat higher than that of Deodeok under the same conditions (Hwang et al., 2011).

Bellflower has been traditionally used for bronchial diseases, and according to the document, Donggibogam and herbaceous tree, it was used for expectoration, drainage, antitussives, tonsillitis, rhyme, etc. . Lee et al. (2000) reported that bronchial microorganisms include Mycobacterium sp . Staphylococcus aureus , Klebsiella pneumeniae , Corynebacterium diphtheriae and Streptococcus pyogenes .

In general, bacteria differ from the structures and components observed in the cell walls of Gram positive and negative bacteria. Mycobacterium spp. Are characterized by having to be cultured for a long time.

Cells of Mycobacterium spp. Contain a large amount of mycolic acid and are not stained well by the general staining method, but once stained, they are anti-acid bacteria because they do not discolor with acid or alkali. Gram-positive bacillus, non-motile, aerobic, no follicles and slow development (Lee et al. 2001).

S. aureus is a Gram-positive bacterium that causes purulent disease. This is because about 30% of healthy people have germs in the nasal cavity and throat skin, and about 35% is contaminated in air samples.These can spread through the nasal passages to the skin and increase infections due to horses, sneezing, and coughing. (Chung et al., 1993).

K. pneumoniae is a pneumococcal bacterium that closely resembles Aerobacter aerogenes . Most causes of infection in the hospital, also known as Friedlander rod bacteria. It is usually seen in children, the elderly, alcoholic, immunocompromised, and especially in leukopenia. As with other pneumonia, the symptoms appear as bronchial pneumonia, releasing bloody jelly-like mucus phlegm. Initially, pus formation and tissue necrosis cause fatal consequences (Ji-Geun, 2004).

C. diphtheriae , a diphtheria bacterium, is a specific pathogen of diphtheria that produces strong exotoxins. It is divided into three types: mitis, gravis, and intermedius. Diphtheria caused by the fungal infection is a statutory epidemic in children. It is a 0.3-0.8 × 1.0-8.0 ㎛ Gram-positive rod, with one end being swollen. Diphtheria exotoxin has a molecular weight of 62,000 and is divided into A and B subunits to inhibit protein synthesis. It is usually infected by the upper airway mucosa by splash infection, and after 2 to 7 days incubation period, mucosal necrosis is caused by toxin, forming an off-white false membrane around the throat. These false membranes do not fall well and are manifested by airway obstruction or toxinemia (Ji Je-geun, 2004).

St. Pyogenes are an important species of Gram-positive extracellular pathogens. It is located in the throat, skin, etc. and causes purulent infection and non purulent sequelae. It is the most common cause of bacterial sore throat and causes scarlet fever and impetigo. It also causes toxic shock syndrome and has recently been reported to cause severe infections involving skin and soft tissues (Song et al., 2004).

Studies on the antibacterial effect of bellflower extract using bacteria isolated from sputum of bronchial and asthmatic patients showed that ehtyl ether extract showed more than 80% antibacterial activity against bronchial bacteria. Other extracts were different depending on the solvent, but it was reported that a number of pharmacologically useful substances that inhibit the growth of bacteria were included (Lee et al., 2000).

Inflammation is one of biological tissues' defense responses to certain stimuli. It is known as a complex lesion that involves three kinds of tissue degeneration, circulatory disorder and exudation, and tissue proliferation. (Park et al., 2004).

Inflammatory processes produce nitric oxide (NO) produced by inducible nitric oxide synthase (iNOS) and prostaglandin E ₂ (PGE ₂ ) produced by cyclooxygenase-2 (COX2). Formation of these inflammatory mediators is known to activate nuclear factor-κB (NF-κB), an inflammatory transcript, and to produce NO by converting arachidonic acid to prostaglandin (PG) (Jeon et al., 2009).

NO (Nitric oxide) is a substance produced when cells infected with bacteria exhibit their own defense mechanism by acting as an active oxygen species (ROS) or an active oxygen intermediate in the immune system in vivo. To form nitrogen peroxide (Park et al., 2004). Lipopolysaccharide (LPS) is an endotoxin present in the extracellular membrane of Gram-negative bacteria and is known to increase various inflammatory cytokines in macrophage or monocytes such as RAW 264.7 cells (Freudenberg, 1988).

Kim et al. (2009) studied the anti-inflammatory activity of the bellflower fraction as an acute inflammation model, and compared it with the existing anti-inflammatory drug ibuprofen 200mg / kg. As a result, we confirmed the acute anti-inflammatory effect of methanol extract and butanol fractionation layer, it was confirmed that there is an anti-edema effect on carrageenan-induced edema, a representative model of acute inflammatory response. In addition, the anti-inflammatory activity in the butanol fraction was confirmed to be due to crude saponin.

In a similar study, the effects of gil saponin fractions on the anticancer activity of macrophages showed a significant concentration-dependent effect on phagocytosis, anticancer and anti-inflammatory effects (Choung et al., 2011).

( Example )

1. Materials and Methods

The bellflower used in the present invention used three years old cultivated in Yeongju, Gyeongsangbuk-do. The black bellflower was steamed at 60 ° C. for 15 days in a thermostat (MCJ-550P2A, MaMa), and dried for 3 hours at 30 ° C. using a dry oven (Vision scientific co., Korea) to produce black bellflower.

The prepared black bellflower and fresh bellflower were used while stored in a -42 ° C deep freezer (MDF-415, Sanyo, Tokyo, Japan).

2. Analysis of Physicochemical Properties of Black Bellflower

1) General Ingredient Analysis

Moisture was measured using a moisture analyzer (FD-720, Kett, Tokyo, Japan), crude fat was measured by a crude fat automatic extractor (Soxtec 2050, Foss, Hoganas, Sweden), and crude ash was measured by direct painting. Crude protein was measured by Kjeldahl method according to the Ministry of Health and Welfare (1995), crude fiber was measured using a crude fiber automatic extractor (Fiber test F-6, Raypa, Barcelona, Spain). The carbohydrate was 100% of the whole sample and the carbohydrate content (%) was obtained by subtracting the moisture, crude protein, crude fat, crude ash and crude fiber content (%).

3. Functional analysis of black bellflower

1) extract manufacturer

The extract for measuring the functionality of the bellflower was mixed with 400 mL of solvent (tertiary distilled water, 80% Methanol) per 40 g of the bellflower sample, and then mixed well.The hot water extract was shaken at 70 ° C. and the methanol extract was shaken at 60 ° C. Extracted for 5 hours. Each extract obtained by repeating this process twice was filtered through filter paper (Whatman No. 1, Maidstone, England) and concentrated under reduced pressure (R-124, Buchi, Flawil, Swizerland), and each concentrated extract was freeze-dried ( FD-1, Eyela, Tokyo, Japan) to make a certain amount of concentration was used in the experiment.

3) Antimicrobial Activity of Black Bellflower Extract

(1) Use strain and medium

Microorganisms used in the present invention are those that are mainly isolated from sputum of bronchial diseases (Lee et al., 2000), Mycobacterium sp . KCTC 1829, Staphylococcus aureus KCTC 1928, Klebsiella pneumoniae KCTC 2245, Corynebacterium diphtheriae KCTC 3075, Streptococcus pyogenes KCTC 3096 was purchased and sold from Korea Research Institute of Bioscience and Biotechnology. Also common bacteria are Escherichia coli ATCC 43894, O157: H7 and Bacillus cereus ATCC 13061 were used. The medium used was Mycobacterium sp . For KCTC 1829, Medium for KCTC 1829 was used to combine the ingredients. For S. aureus KCTC 1928 and K. pneumoniae KCTC 2245, Nutrient Agar and C. diphtheriae KCTC 3075 were Rabbit Blood Agar . Pyogenes KCTC 3096 used Brain heart Infusion Agar.

(2) Antibacterial activity measurement

Strains in continuous culture were diluted to an initial bacterial count of 10 ⁴ -10 ⁵ CFU / mL, inoculated with each strain in sterilized PCA medium, mixed well, aliquoted in a sterilized plate, slightly hardened, and placed on the surface of the plate. I was. Inoculate 50 ml of bellflower extract prepared in different concentrations into sterilized glass cylinders at 4 ℃ refrigeration temperature for 3 hours so that the extract is sufficiently absorbed, and incubate in 37 ℃ incubator for 24 ~ 48h to clear zone around the cylinder. , mm) was measured to compare the antimicrobial activity of each extract.

4) Cell cytotoxicity and anti-inflammatory activity of bellflower extract

(1) Cell line and cell culture

The culture of each cell used in this experiment was performed using DMEM (Dulbeco's Modified Eagle's Medium) medium containing 10% FBS and 1% penicillin / streptomycin (100 U / mL), and 5% CO ₂ incubator at 37 ° C (MCO-17). AIC, Sanyo, Tokyo, Japan) was adapted for subculture.

(2) Cytotoxicity measurement by MTT assay

Cytotoxicity measurements were determined according to the method of Carmichael (1987). Cell line marcrophage (Raw 264.7) cells were dispensed in 0.18 mL into a 96 well plate at 2 × 10 ⁴ cells / well. Samples were prepared by concentration, then 0.02 mL was added. 37 ° C., 5% CO ₂ incubator (MCO-17 AIC , Sanyo, Tokyo, Japan) for 24 hours. The control group was incubated under the same conditions by adding the same amount of distilled water. To this was added 0.02 mL of a solution of MTT (3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-2H-tetrazoliumbromide, Sigma, MO, USA) prepared at a concentration of 5 mg / mL for 4 hours. After incubation, the culture medium was removed, and 0.15 mL of DMSO (dimethyl sulfoxide, Sigma, MO, USA) was added to each well, followed by reaction at room temperature for 30 minutes, followed by absorbance at 540 nm with an ELISA reader (VICTOR ^TM X3, Perkin Elmer, CA, USA). Was measured. Cytotoxicity measurement was expressed as the absorbance decrease rate of the sample solution addition group and no addition group.

(3) NO concentration measurement

Nitric oxide (NO) was measured by the amount of nitric oxide (NO) in the cell supernatant as nitrite and griess. A stable form of griess reagent (Sigma, MO, USA) after reduction to nitrate for nitrite was used. When 5 × 10 ⁵ cells were confluence in a 96 well plate at 80%, washed twice with PBS, and then cultured for 12 hours using a serum-free medium. LPS 1 ㎍ / mL was added to all wells except the control group to stimulate, and after 2 hours the sample was treated by concentration. The amount of NO produced was measured by absorbance at 540 nm after supernatant was collected for 24 hours and reacted with griess reagent for 10 minutes. The amount of NO generated in the group to which only LPS was added was expressed as 100% in terms of absorbance measured when the sample was added.

Ⅳ. Results and Discussion

1. Physicochemical Properties of Black Bellflower

1) General Ingredient

The results of the general components of black bellflower and raw bellflower are shown in FIG. 3. The content of black bellflower was high in all general ingredients except water. In terms of items, the water content of raw bellflower was significantly higher at 78.08%, and the water content of black bellflower was 73.96%. This is presumed to be the result of evaporation of moisture during the steaming process. These results were similar to the water content of the bellflower reported by Chung et al. (1997). In the case of ash content, the black bellflower was 3.05%, which was about 2 times higher than the raw bellflower ( p <0.05), and the crude fiber content was 2.93% of the black bellflower and 2.97% of the raw bellflower. There was no significant difference. Crude fat, crude protein, and carbohydrate contents of the black bellflower were 1.15%, 2.58%, and 16.32 %%, respectively, higher than those of raw bellflower, but there was no significant difference. This result is thought to be due to the decrease in water content, and the tendency of increase and decrease of all items was similar to that of Park (2010) general component of Hongdoraji.

3) Antimicrobial Activity of Black Bellflower Extract

The antimicrobial activity of each extract of the bellflower is shown in FIGS. 4, 5, 6, and 7, respectively. There was a slight difference among the fungi of all species, but the hot water extract of black bellflower showed the best antibacterial effect, and the fresh water of the raw bellflower showed the lowest antibacterial activity.

In the case of the raw water extract of raw Dorage, the clear zone could not be confirmed at all concentrations of 10 mg / mL, and the highest antimicrobial activity was obtained from the 7 strains of C. diphtheriae . In the case of the black water, the hot water extract showed the highest antimicrobial activity in three strains of Mycobacterium , S. aureus and K. pneumoniae , which were involved in bronchitis. Unlike other extracts, the raw bellflower methanol extract was able to identify relatively clear zones at low concentrations and showed the strongest antimicrobial activity in E. coli among common bacteria. Methanol extract of black bellflower showed the highest activity in B. cereus . According to the extraction solvent, the hydrothermal extract showed a slightly higher antimicrobial activity tendency than the methanol extract. The hot water extract of bellflower is believed to contain a large number of saponin components, which is believed to have high antibacterial activity. Bellflower saponin belongs to pentacyclic oleanane type triterpenoid saponin. The saponin effects of bellflower are reported on the effects of crude platycodin reported by Takagi & Lee (1972) and on the defense against infection of E. coli reported by Kim et al. (1988). In addition, there is a study on the enlargement of abscess against S. aureus 226 of 50% MeOH extract reported by Ozaki (1995).

4) Cell cytotoxicity and anti-inflammatory activity of black bellflower extract

(1) Confirmation of survival rate of macrophage cell

The results of confirming the toxicity of macophage cells (Raw264.7) by the bellflower extract by MTT assay are shown in Figure 8. Whether the bellflower extract reduced LPS-induced nitric oxide production or resulted from a decrease in cell population due to the cytotoxicity of the compounds was determined. As a result, in the case of fresh raw water extract, the cell viability was significantly decreased at the concentration of 600 ㎍ / mL, indicating the cytotoxicity. In the case of the fresh raw methanol extract, the cell viability was significantly increased at the concentration of 900 ㎍ / mL. Reduced to a survival rate of 24.99%. Methanol extract of black bellflower showed a slower survival rate than raw bellflower, but showed weak cytotoxicity at 900 ㎍ / mL (72.75%), whereas black bellflower hot water extract showed 98.31% of cells even at the highest concentration of 900 ㎍ / mL. Survival and no cytotoxicity.

(2) Nitric Oxide Inhibitory Activity

In order to measure the degree of NO production inhibition of Raw 264.7 cells, the result of measuring the amount of NO produced by treating the bellflower extract cells by concentration is shown in FIG. 9. Stimulated NO expression by LPS, LPS treated group showed higher NO expression than LPS untreated group. The addition of raw bellflower hot water extract reduced NO synthesis by 67.19% at 300 μg / mL. However, this high NO inhibition may be due to cytotoxicity, as seen in the MTT assay. Methanol extract was significantly reduced to 76.34% at the concentration of 300 sg / mL, but the cytotoxicity is thought to be the result of high cytotoxicity as in the raw water extract. The addition of the Black Dorage hot water extract showed a concentration-dependent inhibition and the lowest inhibition rate among the four sample groups. However, it is the safest sample group in the case of black bellflower hot water extract because it is measured in the range where there is no cytotoxicity.

As described in detail above, the present invention measured the quality characteristics and physiological activity after preparing black bellflower extract through steaming in order to increase and diversify the usability of bellflower having various physiological activities. same.

The moisture content of the common components was lower than that of black bellflower (73.96%) and the crude fiber was 2.93%, but there was no significant difference. Crude fat, crude ash, and crude protein contents were 1.15%, 3.05%, and 2.58%, respectively. In the case of carbohydrate content, the black bellflower was 16.32%.

2. The antimicrobial activity of bellflower extracts was slightly different among the fungi, but the black bellflower hot water extract showed the highest antimicrobial activity among three bronchitis-related microorganisms, including two common bacteria. The hydrothermal extract showed higher antibacterial activity than the methanol extract of each sample.

3. The cytotoxicity of bellflower extract was measured by MTT assay. Toxicity of macrophages was present at 500 and 750 µg / mL concentrations of fresh bellflower. The NO inhibitory activity was slightly better when the raw bellflower extract was compared with the black bellflower extract, but this may be due to cytotoxicity.

As a result of analyzing the quality characteristics and physiological activity of the black bellflower extract prepared by the present invention as described above showed a variety of nutrients, high antioxidant and antimicrobial activity, including a high saponin content compared to raw bellflower. These results will enhance the food value of the black bellflower and promote the use as a functional food.

Although shown and described in connection with a preferred embodiment for illustrating the principles of the present invention as described above, the present invention is not limited to the configuration and operation as shown and described. That is, those skilled in the art to which the present invention pertains will appreciate that many changes and modifications can be made to the present invention without departing from the spirit and scope of the appended claims. Accordingly, all such suitable changes and modifications and equivalents should be considered to be within the scope of the present invention.

Black bellflower extract of the present invention is an invention excellent in utility as a health functional food material.

Claims (2)

Steaming the washed bellflower at 60 ° C. in a thermostat for 15 days,
The process of obtaining a black bellflower by drying the bellflower after the steaming process for 3 hours at 30 ℃ using a dry oven,
The process of obtaining hot water extract by uniformly mixing 400 mL of solvent (tertiary distilled water) per 40 g of the dried black bellflower after the drying process;
The hot water extract is extracted for 5 hours in a shaking water bath of 70 ℃ (shaking water bath),
The extract obtained by repeating the extraction process twice is filtered and filtered under filter paper (filter paper) and concentrated under reduced pressure;
The concentrated extract under reduced pressure is a method for producing black bellflower extract, characterized in that the freeze-dried. Steaming the washed bellflower at 60 ° C. in a thermostat for 15 days,
The process of obtaining a black bellflower by drying the bellflower after the steaming process for 3 hours at 30 ℃ using a dry oven,
The process of obtaining hot water extract by uniformly mixing 400 mL of solvent (tertiary distilled water) per 40 g of the dried black bellflower after the drying process;
The hot water extract is extracted for 5 hours in a shaking water bath of 70 ℃ (shaking water bath),
The extract obtained by repeating the extraction process twice is filtered and filtered under filter paper (filter paper) and concentrated under reduced pressure;
Black bellflower extract, characterized in that prepared by freeze drying the concentrated extract.

Images