KR20120017811A - Antibacterial composition against helicobacter pylori comprising the extract of artemisia and the extract of nelumbo nucifera gaertner as an active ingredient - Google Patents

Abstract

PURPOSE: An antibacterial composition containing Artemisia asiatica and lotus root extract is provided to ensure antibacterial activity against helicobacter pylori and to be used in a cosmetic product and cleanser. CONSTITUTION: An antibacterial composition against helicobacter contains Artemisia asiatica and lotus root extract as an active ingredient. The helicobacter is helicobacter pylori. The extract is prepared using water, alcohol of 1-6 carbon atoms, or mixture solvent thereof mixed in a ratio of 1:0.1- 9. The Artemisia asiatica includes leaves or young stems of Artemisia argyi Lev. et Vant., A. princeps Pamp. var. orientalis Hara, or A. montana Pampani.

Description

Antibacterial composition against Helicobacter pylori comprising the extract of Artemisia and the extract of Nelumbo nucifera Gaertner as an active ingredient}

The present invention relates to an antimicrobial composition containing the leaf extract and lotus root extract as an active ingredient, and more particularly, to an antimicrobial composition having an antimicrobial effect against Helicobacter containing the leaf extract and lotus root extract as an active ingredient.

Strickland and McKay classified chronic gastritis into types A and B according to their anatomical site and cause. Gastritis B is common in all governments and is associated with 70% of gastric ulcer patients and 90% of duodenal ulcer patients. Hepatitis B is known to be associated with Helicobacter pylori infection.

Helicobacter is a gram negative bacterium known to cause chronic, acute gastritis, gastric ulcer, duodenal ulcer and gastric cancer in the human body.

Helicobacter pylori was discovered in 1983 by Warren and Marshall in the gastric mucosa of gastritis patients. In 1994, it was declared a definite carcinogen by the International Agency for Research on Cancer (IARC). Previously, gastrointestinal diseases such as gastritis and gastric ulcer have been known to be caused by excessive acidity due to stress, food and genetic factors, but recently, inflammation caused by Helicobacter pylori infection has been recognized as a major cause. In developing countries, most of the population is reported to have been infected since childhood. Infants between the ages of two and eight years of age are infected with about 10% annually, so most teenagers are infected. In Korea, the infection rate is over 70% after 20s. Warren and Marshall have reported that Helicobacter pylori has been found in 70% of patients with chronic gastritis and 90% of active gastritis.

When inflammation spreads to the submucosa of the stomach, it develops gastric ulcers, and when infected with Helicobacter, it is reported that the probability of developing gastric cancer is three to five times higher.

Antibiotics are the most common way to remove Helicobacter pylori. The use of antibiotics alone against Helicobacter pylori is known to be ineffective. There are many triple treatments that add two antibiotics to a proton pump inhiitor (PPI). However, this requires two to three weeks of intensive care, causes antibiotic-related side effects, and increases the risk of increased resistance.

Therefore, there is an urgent need to develop alternative antimicrobial substances that can prevent the accumulation of antibiotics in the human body, the emergence of harmful strains having side effects and resistance to antibiotic use.

Artemisia Linne belonging to the Asteraceae family has more than 200 species in the northern hemisphere and 38 species in Korea are reported. Stems and petioles are medicinal, young leaves are edible and ordinary leaves are used to make moxa. White fur is used to make a scratch by making a white rice, also called white tiger (白 蒿), Bongae (蓬 艾), the first (艾草) or Bongho (蓬蒿). Mugwort is used as a hemostatic drug such as nosebleeds, uterine bleeding in oriental medicine, and is effective in digestion, lower abdominal pain, elimination of hookworms and odors, and is said to have an effect on constipation, neuralgia, cold sickness, women's diseases and asthma, and has been used as mugwort tea.

Lotus (Nelumbo nucifera Gaertner) is a perennial aquatic plant belonging to the water lily family. Luo X. et al., Analytica Chemica Acta, 538, pp 129-133, 2005). Traditionally, leaves have been used for fever and detoxification because they are called lower lobes. Seeds and pulp have been used for tonic, hemostatic drugs, nocturnal enuresis, and gynecological diseases. (KHP), pp500, 2000; Kim Chang-min, Chinese Medicinal Dictionary, pp5956-5959, 1998).

However, there has been no report on the antimicrobial effect of Helicobacter pylori mixed with the extracts of the leaf extract and lotus root extract.

The inventors of the present invention have completed the present invention by finding that a mixture of leafy leaf extract and lotus root extract effectively inhibits Helicobacter while studying a new substance capable of inhibiting Helicobacter.

Therefore, an object of the present invention is to provide an antimicrobial composition having an antimicrobial action against Helicobacter containing the leaf extract and lotus root extract as an active ingredient.

In order to achieve the above object, the present invention provides an antimicrobial composition having an antimicrobial action against Helicobacter containing the leaf extract and lotus root extract as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present invention provides an antimicrobial composition having an antimicrobial action against Helicobacter containing the leaf extract and lotus root extract as an active ingredient.

The leaf of the present invention refers to the leaves or young stems of Artemisia argyi Lev. Et Vant. And mugwort (A. princeps Pamp. Var. Orientalis Hara) or A. montana Pampani. Similar plants include A. capillaris Herba and A. iwayomogii Herba. Injin is the name of the plant on the ground of A. capillaris Thunberg. (Korean herbal medicine and herbal medicine standard collection, Korea Food and Drug Administration, 2002).

On the other hand, lotus root refers to the root stem of the perennial herbaceous lotus (Nelumbo nucifera Gaertner) belonging to the water lily family. The main ingredient is sugar, most of which is starch. Amino acids include aspartic acid, arginine, and tyrosine, as well as lecithin and pectin. It is also characterized by low levels of vitamin B12 in common plants. Lotus root has been used for fever poisoning, bovine blood, monoemia, bleeding, intestinal bleeding and hemostasis.

Leaf extract and lotus root extract of the present invention can be prepared by a solvent extraction method known in the art. Examples of the extraction solvent include any one selected from alcohols having 1 to 6 carbon atoms such as water, ethanol and methanol, organic solvents such as acetone, ethyl acetate, n-nucleic acid, diethyl ether, acetone, and benzene, or a mixture thereof. Solvents may be used. Preferably, the solvent may be extracted with any one solvent selected from the group consisting of water, alcohols having 1 to 6 carbon atoms, and mixed solvents thereof.

Most preferably, the extraction solvent may be a mixed solvent of ethanol and water. As the extraction method, conventional extraction methods such as cold needle, warm needle and heating may be used using the solvent. When the extraction using a mixed solvent of ethanol and water is not particularly limited the ratio of the root leaf or lotus root and mixed solvent, the mixed solvent may be added to the leaf or lotus root 3 to 20 times by weight. Preferably, in order to increase the extraction efficiency, a mixed solvent may be added in an amount of 8 to 12 times with respect to the root leaf or lotus root. The temperature at the time of extraction is not particularly limited as long as the extraction component is not destroyed, but preferably 4 ° C to 120 ° C. Most preferably 18 ° C. to 30 ° C. The extraction time depends on the extraction temperature and the extraction solvent but is extracted for 0.5 hours to 48 hours, preferably 20 hours to 30 hours.

The leaflets or lotus roots used for extraction can be harvested, washed and used as is or dried. As a drying method, both dry, shade, hot air drying and natural drying can be used. In addition, in order to increase the extraction efficiency can be used by grinding the leaflets or lotus root with a grinder.

The extract of the present invention includes not only the extract by the aforementioned extraction solvent, but also an extract that has undergone a conventional purification process. For example, sediment separation using filter paper, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity), decolorization process, etc. Fractions obtained through various purification methods additionally carried out are also included in the extract of Lotus leaf or lotus root of the present invention. Preferably, the extract of the present invention may be further used after the sediment separation process and concentration process using a filter paper. The concentration process can be concentrated using a known concentration apparatus, or by bathing at a temperature of 100 ℃ or less.

In one embodiment of the present invention, 100 g of rosemary or lotus root was added to 1 L of water or 20% ethanol, extracted at 24 ° C. for 24 hours, and concentrated to prepare a leaf extract and lotus root extract (see Example 1).

The leaf extract and lotus root extract contained in the composition may be used in the form of powder or extract, and the amount and ratio may be variously adjusted according to the amount of leaf and lotus root used for extraction, the degree of extract concentration, and the use. The composition of the present invention is not particularly limited, but the mixing ratio of the leaf extract and lotus root extract is preferably used in a mixed state in a ratio of 1: 0.1 to 9 and more preferably 1: 0.1 to 1.5 ratio It can be used in a mixed state and most preferably the leaf extract and lotus root extract can be used in a mixed ratio of 4: 1.

The composition of the present invention is characterized by showing antimicrobial activity against Helicobacter.

The antimicrobial effect of the composition of the present invention was confirmed by an antimicrobial activity test using Helicobacter pylori in one embodiment of the present invention.

In one embodiment of the present invention, after inoculating Helicobacter pylori in agar medium, the disc paper is placed, and a mixed composition of a leaf extract and lotus root extract in a ratio of 1 to 1 is administered to form a culture inhibitory ring of harmful microorganisms. Was observed. As a result, it was confirmed that the inhibitory ability is much superior to the group administered alone or lotus root extract alone (see Example 2).

In another embodiment of the present invention to prepare a composition of the present invention by varying the mixing ratio of the leaf extract and lotus root extract to measure the antimicrobial activity against Helicobacter pylori.

As a result, the mixed composition of the present invention was confirmed that the overall antimicrobial activity is excellent, especially when the mixing ratio of the leaf extract and lotus root extract is 4: 6 to 9: 1 (1: 1.5 to 1: 0.1), 8: It was confirmed that the best antimicrobial activity against Helicobacter when 2 (see Example 3).

Therefore, the present invention provides an antimicrobial composition containing the leaf extract and lotus root extract as an active ingredient.

The composition of the present invention has an excellent antimicrobial effect against Helicobacter, and particularly has an excellent antimicrobial activity against Helicobacter pylori (Helicobacter pylori).

In addition, the composition of the present invention is harmless to the human body because it is extracted from plants used for edible, medicinal, and does not cause side effects from long-term use.

The antimicrobial composition of the present invention is preferably a fluid, a solid, a powder, an emulsion, a gel or a capsule, but may be applied differently depending on the intended use. The antimicrobial composition is preferably carried out once to three times at 0.000001 to 1000 mg per day, but more preferably adjusted according to the state of use and application site.

Therefore, the present invention provides an antimicrobial composition against Helicobacter containing the leaf extract and lotus root extract as an active ingredient. The composition of the present invention is excellent in antimicrobial ability against Helicobacter pylori can be usefully used in the form of cosmetics, disinfectants, cleaning agents and food preservatives.

1 is a photograph comparing the antimicrobial activity of the composition of the present invention Helicobacter pylori.
Figure 2 is a photograph of the results of testing the antimicrobial activity against Helicobacter pylori by varying the mixing ratio of the lotus root extract and leaf extract of the composition of the present invention.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

< Example  1>

Love  And lotus root extract

Artemisia argyi Levl. Et vant was dried and used for medicinal herbs in Gyeongdong market, and lotus root was purchased in Heukseok-dong market for drying.

<1-1> Love  And extract of lotus root water

100 g of leaflets or lotus root was added to 1000 ml of first distilled water, and the mixture was left at 24 ° C. for 24 hours to extract the extract. The extract was separated from the sludge and the solution by using a filter paper (Whatman filter paper), and the separated solution was used by condensing at a temperature of 95 ° C. and concentrating 4 times.

<1-2> Love  And ethanol extract of lotus root

1000g of 20% ethanol and 100g of rosemary or lotus root were mixed and extracted by standing at 24 ° C for 24 hours. The extract was concentrated 4 times at 38 ° C. using a concentrator (Evaporator, Heidolph vv 2000) to separate the sludge and the solution using Whatman filter paper and remove ethanol from the separated solution.

< Example  2>

Love  Antimicrobial Effect Test of Extracts and Lotus Root Extracts

Helicobacter pylori ATCC 43504 was sold by Chung-Ang University Hospital. Helicobacter pylori was enriched in a 10% CO ₂ incubator on BAP (blood agar plate, AM601-03, Asan Pharmaceutical, Seoul) containing 10% sheep blood.

Helicobacter pylori cultured for 3 to 4 days was prepared by suspending in 0.85% saline to adjust the viable cell number to about 10 ⁷ . The experimental medium was sterilized with Mueller hinton broth agar (MHB, Difco, USA), cooled to 40 ° C, and FBS (fetal bovine serum) was added to 10% of the total volume. A Helicobacter pylori agar plate was prepared by mixing the prepared Helicobacter pylori with a viable cell number 10 ⁷ . Three 10 mm paper disks (Toyo Roshi Kaisha, Ltd, Japan), dried for 24 hours in a 56 ° C incubator and completely dehumidified, were placed on the plate, and then each of the leaf extracts, lotus root extracts and lotus root extracts and leaf extracts 100 μl of the mixed composition was administered to each other, and cultured in a 37 ° C., 10% CO ₂ incubator for 3 to 4 days was observed to form an inhibitory ring. Helicobacter pylori was confirmed by dropping 0.1% NNNN-tetra phnylene diamine on the medium and measuring the size of the site that does not turn purple (Oxidase test).

Results of antimicrobial activity of the composition of the present invention Helicobacter pylori suppressor ring size (mm) Lotus root Lotus root + lobe Love 10 28 26

As a result, as shown in [1] and [Table 1], the mixed composition of the leaf extract and lotus root extract of the present invention was confirmed that the antibacterial activity of inhibiting the growth of Helicobacter pylori, excellent lotus root extract or leaf extract alone It was confirmed that the efficacy is much superior to the use.

< Example  3>

Love  Antimicrobial Effect Test According to the Mixing Ratio of Extracts and Lotus Root Extracts

The change of antimicrobial activity against Helicobacter pylori of the composition according to the ratio of the leaf extract and lotus root extract was measured.

Helicobacter pylori agar plate for measuring antimicrobial activity was prepared according to the method in <Example 2>.

9 dry 10 mm paper disks were placed on a plate, and the leaf extracts and lotus root extracts prepared in Example 1 were mixed according to the composition of [Table 2], and then administered to each disk. After incubation for 3 days to 4 days at 0.1% NNNN-tetra phnylene diamine using the Oxidase test using an oxidase test was observed whether the ring formation.

Results of antimicrobial activity according to the extract mixing ratio of the composition of the present invention Experiment number One 2 3 4 5 6 7 8 9 Lotus root extract: leaf ratio 9: 1 8: 2 7: 3 6: 4 5: 5 4: 6 3: 7 2: 8 1: 9 Ring size (mm) 17 16 17 20 21 23 24 26 25

As a result, as shown in [Fig. 2] and [Table 2], it was confirmed that the mixed composition of the present invention is excellent overall antimicrobial activity, the mixing ratio of the leaf extract and lotus root extract is 4: 6 to 9: 1 (1: 1.5 to 1: 0.11) was particularly excellent, and when 8: 2, it was confirmed that the antibacterial activity against Helicobacter was the best.

As described above, the present invention provides an antimicrobial composition against Helicobacter containing the leaf extract and lotus root extract as an active ingredient. The composition of the present invention is excellent in the antimicrobial ability against Helicobacter pylori can be usefully used in the form of cosmetics, disinfectants, cleaning agents and food preservatives is high industrial applicability.

Claims (4)

An antimicrobial composition having an antimicrobial effect against Helicobacter containing the leaf extract and lotus root extract as an active ingredient.
The composition of claim 1, wherein the Helicobacter is Helicobacter pylori.
The composition according to claim 1 or 2, wherein the leaf extract or lotus root extract is extracted with any one solvent selected from the group consisting of water, alcohols having 1 to 6 carbon atoms and mixed solvents thereof.
The composition according to claim 1 or 2, wherein the leaf extract and lotus root extract are contained in a ratio of 1 to 0.1 to 9.

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