KR20110100316A - Ptp1b 유전자의 발현을 억제하기 위한 조성물 및 방법 - Google Patents
Ptp1b 유전자의 발현을 억제하기 위한 조성물 및 방법 Download PDFInfo
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Abstract
본 발명은 PTP1B 유전자의 발현을 억제하기 위한 이중-가닥 리보핵산(dsRNA)에 관한 것이다. 본 발명은 또한 약학적으로 허용되는 담체와 함께 dsRNA 또는 핵산 분자 또는 이를 암호화하는 벡터를 포함하는 약학 조성물; 상기 약학 조성물을 사용하여 PTP1B 유전자의 발현에 의해 야기되는 질환을 치료하는 방법; 및 세포에서 PTP1B의 발현을 억제하는 방법에 관한 것이다.
Description
본 발명은 이중-가닥 리보핵산(dsRNA), 및 PTP1B 유전자의 발현을 억제하는 RNA 간섭을 매개하는데 있어 그의 용도에 관한 것이다. 또한, 2형 당뇨병, 간부전 및 비만과 같이 PTP1B 유전자의 발현과 관련된 광범위한 질환/질병을 치료/예방하기 위한 상기 dsRNA의 용도는 본 발명의 일부이다.
2형 당뇨병은 인슐린 저항성, 고인슐린혈증 및 고혈당증을 나타내는 다유전자성 질환이다. 인슐린 저항성의 근간을 이루는 분자 기전은 잘 알려져 있지 않지만, 후-인슐린 수용체(IR) 신호 전달 경로에 결함을 수반하는 것으로 여겨진다. IR은 수용체 티로신 키나제이며, 그 수용체에 인슐린의 결합은 IR의 자가포스포릴화 및 IR 기질 단백질의 티로실 포스포릴화를 야기한다. 단백질 티로신 키나제 및 단백질 티로신 포스파타제는 인슐린 신호 전달의 중요한 조절제이다.
인슐린 수용체에 의해 매개된 신호전달에 있어 필수적인 조절 역할이 PTP1B에 대해 규명되었다. PTP1B(단백질 포스파타제 1B 및 PTPN1으로도 알려져 있음)는 소포체의 세포질면에 편재되며, 간, 근육 및 지방을 포함하여 도처에서 발현된다. 생화학적, 유전학적 및 약리학적 연구는 인슐린 및 렙틴 신호전달 둘 다에 있어 역 조절제로서 PTP1B에 대한 역할을 뒷받침한다. PTP1B는 활성화 인슐린 수용체(IR) 또는 인슐린 수용체 기질(IRS)과 결합하여 이를 탈포스포릴화시킬 수 있다. 세포 배양물에서 PTP1B의 과별현은 IR 및/또는 IRS-1의 인슐린-자극된 포스포릴화를 감소시키는 반면, 세포 및 동물에서 PTP1B 수준의 감소는 인슐린-개시된 신호전달을 증대시킨다. 또한, 양적 형질 유전자좌, 및 인간에서 PTP1B를 암호화하는 유전자에서의 돌연변이에 대한 분석은 PTP1B의 이상 발현이 당뇨병 및 비만의 원인이 될 수 있다는 개념을 뒷받침한다. 당뇨병 및 비만에서 PTP1B의 이러한 중요한 역할은 또한 동물 모델에서도 입증되었다. PTP1B 발현이 결핍된 마우스는 증가된 인슐린 민감성 및 고지방식 공급시 체중 증가에 대한 저항과 함께 낮은 지방축적을 나타내었다. 또한, 이들 마우스는 증가된 기초 대사율 및 총 에너지 소비를 나타낸다. 흥미롭게도, PTP1B는 그 결과 렙틴 수용체 하위에 있는 JAK2에 결합하여 이를 탈포스포릴화시키는 것으로 나타났다. 따라서, PTP1B-/- 마우스에서 관찰된 식이-유발 비만에 대한 저항은 증대된 렙틴-민감성으로 인한 증가된 에너지 소비와 관련되기 쉽다.
흥미롭게도, PTP1B-/- 마우스에서 PTP1B의 간-특이적 재발현은 감소된, 인슐린 수용체의 인슐린-자극 티로실 포스포릴화 및 IRS-2-결합 포스파티딜이노시티드 3-키나제 활성과 관련하여 그의 증대된 인슐린 민감성의 현저한 감소를 야기하여, 간이 글루코스 항상성을 조절하는데 있어 PTP1B의 말초 작용의 주요 부위임을 입증하였다.
최근 수년간, 먼저 형태학적으로 규정된 과정인 세포 사멸(자연세포사)은 배아 발생, 자가반응성 T-세포의 결실 및 성인 조직 항상성에 중요한 역할을 하는 고도로 조절된 형태의 세포 사멸임이 명백해졌다. 자연세포사 프로그램의 교란이 간 질환을 포함하여 일련의 질환의 근본적인 원인이라는 증거가 증가하고 있다. 세포 증식 및 세포 사멸은 자극 및 억제 신호에 의해 좌우된다. 영양 인자가 체세포분열을 자극하는 동시에 세포 사멸을 억제하는 반면, 역 성장 신호는 이들 생물학적 효과의 반대효과를 조절한다. 간에서, 영양 인자로는 내인성 성장 인자, 예를 들면, 티로신 키나제 상과에 속하는 수용체를 통해 작용하는 EGF, bFGF, TGF-β 및 IGF가 포함된다. 티로신 키나제 및 PTP의 억제는 또한 간에서 자연세포사를 조절할 수 있다. PTP1B의 결여가 간에서 사전-자연세포사 유전자의 내인성 발현에 상당한 변화를 유발하기 때문에, PTP1B를 표적으로 하는 본 발명의 dsRNA는 간 부전의 치료에 사용될 수 있다.
총괄하여, 이러한 생화학적, 유전학적 및 약리학적 연구는 유력한 개념 증명을 제공하여, PTP1B의 억제가 당뇨병, 간부전 및 비만을 해결할 수 있다는 개념을 입증하고 PTP1B가 약물 개발에 흥미로운 대상이 되게 한다.
이중-가닥 리보핵산(dsRAN) 분자는 RNA 간섭(RNAi)으로 알려진 고도로 보존된 조절 기전에서 유전자 발현을 차단하는 것으로 나타났다. 본 발명은 PTP1B의 발현을 선택적으로 및 효과적으로 감소시킬 수 있는 이중-가닥 리보핵산(dsRNA)을제공한다. PTP1B RNAi의 사용은 인슐린 및 렙틴 신호전달과 관련되는 질환/질병의 치료 및/또는 예방적 치료를 위한 방법을 제공한다. 특정한 질환/질병 상태는 2형 당뇨병, 비만, 간부전, 이상지질혈증, 당뇨병성 죽상동맥경화증 및 고혈압의 치료 및/또는 예방적 치료를 포함하며, 상기 방법은 인간 또는 동물에게 PTP1B를 표적화하는 dsRNA의 투여를 포함한다.
한 바람직한 태양에서, 기술된 dsRNA 분자는 PTP1B 유전자의 발현을 60% 이상, 바람직하게는 70% 이상, 가장 바람직하게는 80% 이상까지 억제할 수 있다. 본 발명은 또한 PTP1B dsRNA로 간을 특이적으로 표적화하고, 전술한 것을 포함하여 PTP1B 유전자의 발현에 의해 야기된 병적 상태 및 질환을 치료하기 위한 조성물 및 방법을 제공한다.
한 태양에서, 본 발명은 PTP1B 유전자의 발현, 특히 포유동물 또는 인간 PTP1B 유전자의 발현을 억제하기 위한 이중-가닥 리보핵산(dsRAN) 분자를 제공한다. dsRNA는 서로 상보성인 2개 이상의 서열을 포함한다. dsRNA는 제 1 서열을 포함하는 센스 가닥 및 제 2 서열을 포함할 수 있는 안티센스 가닥을 포함한다(서열표에 제공된 서열 및 또한 첨부된 표 1 및 4에 제공된 특정 dsRNA 쌍 참조). 한 태양에서, 센스 가닥은 PTP1B를 암호화하는 mRNA의 적어도 일부와 90% 이상의 동일성을 갖는 서열을 포함한다. 상기 서열은 안티센스 가닥에 대한 센스 가닥의 상보성 영역에, 바람직하게는 안티센스 가닥의 5' 말단의 뉴클레오티드 2 내지 7내에 위치한다. 한 바람직한 태양에서, dsRNA는 특히 인간 PTP1B 유전자를 표적화하며, 또 다른 바람직한 태양에서 dsRNA는 마우스(무스 무스큘러스(Mus musculus)) 및 래트(래터스 노르베기커스(Rattus norvegicus)) PTP1B 유전자를 표적화한다.
한 태양에서, 안티센스 가닥은 상기 PTP1B 유전자를 암호화하는 mRNA의 적어도 일부분에 실질적으로 상보성인 뉴클레오티드 서열을 포함하며, 상보성 영역은 가장 바람직하게는 길이가 30개 뉴클레오티드 미만이다. 또한, 본원에 기술된 본 발명의 dsRNA 분자의 길이(이중 길이)는 약 16 내지 30개 뉴클레오티드의 범위, 특히 약 18 내지 28개 뉴클레오티드의 범위인 것이 바람직하다. 본 발명에 있어서 약 19, 20, 21, 22, 23 또는 24개 뉴클레오티드의 이중 길이가 특히 유용하다. 19, 21 또는 23개 뉴클레오티드의 이중 연장부가 가장 바람직하다. dsRNA는 PTP1B 유전자를 발현하는 세포와 접촉시 시험관내에서 PTP1B 유전자의 발현을 60% 이상, 바람직하게는 70% 이상, 가장 바람직하게는 80% 이상을 억제한다.
첨부된 표 13은 본 발명에 따라 dsRNA로 사용될 바람직한 분자에 관한 것이다. 또한 변형된 dsRNA 분자도 본원에서 제공되며 특히 본 발명의 변형된 dsRNA 분자의 실례를 제공하는 첨부된 표 1 및 4에 개시되어 있다. 본원에서 상기에 지적한 바와 같이, 표 1은 본 발명의 변형된 dsRAN의 실례를 제공한다(여기서 상응하는 센스 가닥 및 안티센스 가닥이 상기 표에 제공되어 있다). 표 1의 변형된 dsRNA에 대한 표 13에 나타낸 변형되지 않은 바람직한 분자의 관계는 표 14에 예시되어 있다. 또한, 여기에는 본 발명의 dsRNA의 상기 구성성분의 예시적인 변형이 변형의 예로서 제공되어 있다.
표 2 및 3은 본 발명의 특정 dsRNA 분자의 선택적인 생물학적, 임상적 및 약학적 관련 파라미터를 제공한다.
첨부된 표 13에는 가장 바람직한 dsRNA 분자가 제공되어 있으며, 무엇보다 바람직하게, 여기서 센스 가닥은 서열번호 630, 632, 634, 638, 640, 644 및 652에 나타낸 핵산 서열로 이루어진 군에서 선택되고, 안티센스 가닥은 서열번호 631, 633, 635, 639, 641, 645 및 653에 나타낸 핵산 서열로 이루어진 군에서 선택된다. 따라서, 본 발명의 dsRNA 분자는 무엇보다 서열번호 630/631, 632/633, 634/635, 638/639, 640/641, 644/645 및 652/653으로 이루어진 군에서 선택된 서열 쌍을 포함한다. 본원에 제공된 특정 dsRNA 분자와 관련하여, 서열번호의 쌍들은 첨부되고 포함된 표들에 또한 나타낸 바와 같이 상응하는 센스 및 안티센스 가닥 서열(5'에서 3')에 관한 것이다.
한 태양에서, 상기 dsRAN 분자는 1 내지 5개 뉴클레오티드 길이, 바람직하게는 1 내지 2개 뉴클레오티드 길이의 3' 돌출부(overhang)를 갖는 안티센스 가닥을 포함한다. 바람직하게, 안티센스 가닥의 상기 돌출부는 우라실, 또는 PTP1B를 암호화하는 mRNA에 상보성인 뉴클레오티드를 포함한다.
또 다른 바람직한 태양에서, 상기 dsRNA 분자는 1 내지 5개 뉴클레오티드 길이, 바람직하게는 1 내지 2개 뉴클레오티드 길이의 3' 돌출부를 갖는 센스 가닥을 포함한다. 바람직하게, 센스 가닥의 상기 돌출부는 우라실, 또는 PTP1B를 암호화하는 mRNA와 동일한 뉴클레오티드를 포함한다.
또 다른 바람직한 태양에서, 상기 dsRNA 분자는 1 내지 5개 뉴클레오티드 길이, 바람직하게는 1 내지 2개 뉴클레오티드 길이의 3' 돌출부를 갖는 센스 가닥, 및 1 내지 5개 뉴클레오티드 길이, 바람직하게는 1 내지 2개 뉴클레오티드 길이의 3' 돌출부를 갖는 안티센스 가닥을 포함한다. 바람직하게, 센스 가닥의 상기 돌출부는 우라실, 또는 PTP1B를 암호화하는 mRNA와 90% 이상 동일한 뉴클레오티드를 포함하고, 안티센스 가닥의 상기 돌출부는 우라실, 또는 PTP1B를 암호화하는 mRNA에 대해 90% 이상 상보성인 뉴클레오티드를 포함한다.
본 발명의 dsRNA 분자는 천연 뉴클레오티드로 이루어질 수 있거나, 또는 하나 이상의 변형된 뉴클레오티드, 예를 들면, 2'-O-메틸 변형된 뉴클레오티드, 5'포스포로티오에이트기를 포함하는 뉴클레오티드, 및 콜레스테릴 유도체 또는 도데카노산 비스데실아미드기에 연결된 말단 뉴클레오티드로 이루어질 수 있다. 2' 변형된 뉴클레오티드는 본 발명의 dsRNA 분자가 생체내, 예를 들면, 의학적 환경에서 사용될 때 특정한 면역조절 인자 또는 사이토카인이 억제된다는 추가의 이점을 가질 수 있다. 대안적으로 및 비제한적으로, 변형된 뉴클레오티드는 2'-데옥시-2'-플루오로 변형된 뉴클레오티드, 2'-데옥시-변형된 뉴클레오티드, 고정(locked) 뉴클레오티드, 염기결여(abasic) 뉴클레오티드, 2'-아미노-변형된 뉴클레오티드, 2'-알킬-변형된 뉴클레오티드, 모르폴리노 뉴클레오티드, 포스포르아미데이트 및 비-천연 염기 포함 뉴클레오티드로 이루어진 군에서 선택될 수 있다. 한 바람직한 태양에서, dsRNA 분자는 다음의 변형된 뉴클레오티드: 2'-O-메틸 변형된 뉴클레오티드, 5'-포스포로티오에이트기를 포함하는 뉴클레오티드 및 데옥시티미딘 중 하나 이상을 포함한다. 변형된 뉴클레오티드를 포함하는 바람직한 dsRNA 분자를 표 1 및 4에 나타내었다.
가장 바람직한 dsRNA 분자를 첨부된 표 1 내지 4에 나타내었으며, 특히, 바람직하게, 여기서 센스 가닥은 서열번호: 3, 5, 7, 11, 13, 17, 25에 나타낸 핵산 서열로 이루어진 군에서 선택되고, 안티센스 가닥은 서열번호: 4, 6, 8, 12, 14, 18 및 26에 나타낸 핵산 서열로 이루어진 군에서 선택된다. 따라서, 본 발명의 dsRNA 분자는 특히 서열번호: 3/4, 5/6, 7/8, 11/12, 13/14, 17/18 및 25/26으로 이루어진 군에서 선택된 서열 쌍을 포함할 수 있다. 본원에 제공된 특정 dsRNA 분자와 관련하여, 서열번호의 쌍은 첨부되고 포함된 표에 또한 나타낸 바와 같은 상응하는 센스 및 안티센스 가닥 서열(5'에서 3')에 관한 것이다.
바람직한 태양에서, 본 발명의 dsRNA 분자는 표 1 및 4에 나타낸 서열에 열거된 바와 같이 변형된 뉴클레오티드를 포함한다. 한 바람직한 태양에서, 본 발명의 dsRNA 분자는 서열번호: 3/4, 5/6, 7/8, 11/12, 13/14, 17/18 및 25/26으로 이루어진 군에서 선택된 서열 쌍을 포함하며, 표 1에 열거된 바와 같은 변형을 포함한다.
또 다른 태양에서, 본 발명의 dsRNA는 표 1 및 4에 개시된 것과 상이한 위치에 변형된 뉴클레오티드를 포함한다. 한 바람직한 태양에서, dsRNA 분자의 두 가닥 모두의 3' 말단에서 2개의 데옥시티미딘 뉴클레오티드가 발견된다.
한 태양에서, 본 발명의 dsRNA 분자는 센스 및 안티센스 가닥으로 이루어지는데, 이때 두 가닥 모두 5 시간 이상의 반감기를 갖는다. 한 바람직한 태양에서, 본 발명의 dsRNA 분자는 인간 혈청에서 5 시간 이상의 반감기를 갖는 센스 및 안티센스 가닥으로 이루어진다. 또 다른 태양에서, 본 발명의 dsRNA 분자는 비-면역조절성이다, 즉, 시험관내에서 INF-알파 및 TNF-알파를 자극하지 않는다. 또 다른 태양에서, 본 발명의 dsRNA 분자는 시험관내에서 매우 미미한 정도로 INF-알파 및 TNF-알파를 자극한다.
본 발명은 또한 하나 이상의 본 발명의 dsRNA를 포함하는 세포를 제공한다. 상기 세포는 바람직하게는 포유동물 세포, 예를 들면, 인간 세포이다. 또한, 본원에 정의된 dsRNA 분자를 포함하는 조직 및/또는 비-인간 유기체도 본 발명에 포함되며, 이때 상기 비-인간 유기체는 연구 목적에 또는 연구 도구로서, 예를 들면, 또한 약물 검사에 특히 유용하다.
또한, 본 발명은 다음 단계를 포함하는, 세포, 조직 또는 유기체에서 PTP1B 유전자, 특히 포유동물 또는 인간 PTP1B 유전자의 발현을 억제하는 방법에 관한 것이다:
(a) 세포, 조직 또는 유기체 내에 본원에 정의된 바와 같은 이중-가닥 리보핵산(dsRNA)을 도입하고;
(b) 단계 (a)에서 생성된 상기 세포, 조직 또는 유기체를 PTP1B 유전자의 mRNA 전사체의 분해를 야기하기에 충분한 시간동안 유지시켜, 해당 세포에서 PTP1B 유전자의 발현을 억제한다.
본 발명은 또한 본 발명의 dsRNA를 포함하는 약학 조성물에 관한 것이다. 이들 약학 조성물은 세포, 조직 또는 유기체에서 PTP1B 유전자의 발현을 억제하는데 특히 유용하다. 하나 이상의 본 발명의 dsRNA를 포함하는 약학 조성물은 또한 (하나의) 약학적으로 허용되는 담체(들), 희석제(들) 및/또는 부형제(들)을 포함할 수 있다.
또 다른 태양에서, 본 발명은 PTP1B와 관련된 2형 당뇨병, 비만, 간부전, 이상지질혈증, 당뇨병성 죽상동맥경화증 및 고혈압을 치료, 예방 또는 관리하기 위한 방법을 제공하며, 상기 방법은 상기 치료, 예방 또는 관리를 필요로 하는 대상에게 치료적으로 또는 예방적으로 효과량의 하나 이상의 본 발명의 dsRNA를 투여하는 것을 포함한다. 바람직하게는, 상기 대상은 포유동물, 가장 바람직하게는 인간 환자이다.
한 태양에서, 본 발명은 PTP1B 유전자의 발현에 의해 매개된 병적 상태를 갖고 있는 대상을 치료하는 방법을 제공한다. 상기 상태는 전술한 바와 같이 당뇨병, 간부전 및 비만과 관련된 질병을 포함한다. 상기 태양에서, dsRNA는 PTP1B 유전자의 발현을 조절하는 치료제로 작용한다. 상기 방법은 본 발명의 약학 조성물을 환자(예를 들면, 인간)에게 투여하여 PTP1B 유전자의 발현을 침묵시키는 것을 포함한다. 그의 높은 특이성으로 인해, 본 발명의 dsRNA는 PTP1B 유전자의 mRNA를 특이적으로 표적화한다. 한 바람직한 태양에서, 기술된 dsRNA는 PTP1B mRNA 수준을 특이적으로 감소시키며, 세포에서 비표적(off-target) 유전자의 발현 및/또는 mRNA 수준에는 직접 영향을 미치지 않는다.
한 바람직한 태양에서, 기술된 dsRNA는 간에서의 PTP1B mRNA 수준을 생체내에서 60% 이상, 바람직하게는 70% 이상, 가장 바람직하게는 80% 이상까지 감소시킨다. 또 다른 태양에서, 기술된 dsRNA는 생체내에서 PTP1B mRNA 수준을 4 일 이상동안 감소시킨다. 또 다른 태양에서, 기술된 dsRNA는 생체내에서 PTP1B mRNA 수준을 4 일 이상동안 60% 이상까지 감소시킨다.
치료적 dsRNA와 관련하여, 동물 또는 세포 배양물 모델에서 독성, 치료 효과 및 효과적 투여량, 및 개개 dsRNA에 대한 생체내 반감기를 평가하기 위해 사용될 수 있는 마우스 및 래트 PTP1B를 표적화하는 dsRNA의 세트가 특히 유용하다.
또 다른 태양에서, 본 발명은 세포에서 PTP1B 유전자, 특히 본 발명의 하나의 dsRAN의 하나 이상의 가닥을 암호화하는 뉴클레오티드 서열에 작동가능하게 연결된 조절 서열을 포함하는 PTP1B 유전자의 발현을 억제하기 위한 벡터를 제공한다.
또 다른 태양에서, 본 발명은 세포에서 PTP1B 유전자의 발현을 억제하기 위한 벡터를 포함하는 세포를 제공한다. 상기 벡터는 본 발명의 하나의 dsRNA의 하나 이상의 가닥을 암호화하는 뉴클레오티드 서열에 작동가능하게 연결된 조절 서열을 포함한다. 또한, 상기 벡터는 상기 조절 서열 이외에, 본 발명 dsRNA의 하나 이상의 "센스 가닥" 및 상기 dsRNA의 하나 이상의 "안티센스 가닥"을 암호화하는 서열을 포함하는 것이 바람직하다. 특허청구된 세포는 상기 조절 서열이외에, 본 발명의 하나의 dsRNA의 하나 이상의 가닥을 암호화하는 본원에 정의된 서열(들)을 포함하는 벡터를 2개 이상 포함하는 것으로 또한 생각된다.
한 태양에서, 상기 방법은 dsRNA를 포함하는 조성물을 투여하는 것을 포함하는데, 이때 상기 dsRNA는 치료될 포유동물의 PTP1B 유전자의 RNA 전사체의 적어도 일부분에 상보성인 뉴클레오티드 서열을 포함한다. 상기에서 지적한 바와 같이, 본원에 정의된 dsRNA 분자의 하나 이상의 가닥을 암호화하는 핵산 분자를 포함하는 벡터 및 세포도 또한 약학 조성물로서 사용될 수 있으므로, 의학적 개입을 필요로 하는 대상을 치료하기 위한 본원에 개시된 방법에 또한 사용될 수 있다. (인간) 대상을 치료하는 약학 조성물 및 상응하는 방법에 관한 상기 태양은 또한 유전자 치료 접근방법과 같은 접근방법에 관한 것임을 또한 주지해야 한다. 본원에 제공된 바와 같은 PTP1B 특이적 dsRNA 분자, 또는 이들 본 발명의 dsRNA 분자의 개개 가닥을 암호화하는 핵산 분자는 또한 벡터내에 삽입되어 인간 환자를 위한 유전자 치료용 벡터로 사용될 수 있다. 유전자 치료 벡터는, 예를 들면, 정맥내 주사, 국소 투여(미국 특허 제 5,328,470 호 참조)에 의해 또는 정위 주사(예를 들면, 문헌 [Chen et al., Proc. Natl. Acad. Sci. USA 91:3054-3057 (1994)] 참조)에 의해 대상에게 전달할 수 있다. 유전자 치료 벡터의 약학 제제는 허용되는 희석제 중에 유전자 치료 벡터를 포함할 수 있거나, 또는 유전자 전달 비히클이 함입되어 있는 서방성 매트릭스를 포함할 수 있다. 또는, 재조합 세포, 예를 들면, 레트로바이러스 벡터로부터 완전한 유전자 전달 벡터가 손상되지 않고 생성되는 경우, 상기 약학 제제는 유전자 전달 시스템을 생성하는 하나 이상의 세포를 포함할 수 있다.
본 발명의 또 다른 태양에서, PTP1B 유전자 발현 활성을 조절하는 PTP1B 특이적 dsRNA 분자는 DNA 또는 RNA 벡터(예를 들면, 스킬런(Skillern, A.) 등의 국제 PCT 공개공보 WO 00/22113 호 참조) 내에 삽입된 전사 단위로부터 발현된다. 상기 전이유전자들은, 숙주 게놈중에 통합된 전이유전자로서 삽입되고 유전될 수 있는 선형 구조물, 원형 플라스미드 또는 바이러스 벡터로서 도입될 수 있다. 상기 전이유전자는 또한 외부염색체 플라스미드로서 유전되게 하도록 구성될 수 있다[Gassmann et al., Proc. Natl. Acad. Sci. USA 92:1292 (1995)].
daRNA의 개개 가닥은 2개의 별도의 발현 벡터 상에서 프로모터에 의해 전사되고 표적 세포내에 동시-형질감염될 수 있다. 또는, dsRNA의 각각의 개개 가닥은, 둘 다 동일한 발현 플라스미드 상에 위치하는 프로모터에 의해 전사될 수 있다. 바람직한 태양에서, dsRNA는 이것이 머리핀(stem and loop) 구조를 갖도록 링커 폴리뉴클레오티드 서열에 의해 연결된 역위 반복체로서 발현된다.
재조합 dsRNA 발현 벡터는 바람직하게는 DNA 플라스미드 또는 바이러스 벡터이다. dsRNA 발현 바이러스 벡터는 아데노-결합 바이러스(검토를 위해, 문헌 [Muzyczka, et al., Curr. Topics Micro. Immunol. 158:97-129 (1992)] 참조); 아데노바이러스(예를 들면, 문헌 [Berkner, et al., BioTechniques 6:616 (1998); Rosenfeld et al. Science 252:431-434 (1991); and Rosenfeld et al. Cell 68:143-155 (1992)] 참조); 또는 알파바이러스 및 당해 분야에 공지된 다른 것들을 기준으로 구성될 수 있으나, 이들로 제한되지는 않는다. 레트로바이러스는 시험관내 및/또는 생체내에서 상피 세포를 포함하여 많은 상이한 세포 유형들에 다양한 유전자를 도입하기 위해 사용되었다(예를 들면, 문헌 [Danos and Mulligan, Proc. Natl. Acad. Sci. USA 85:6460-6464 (1998)] 참조). 세포의 게놈 내에 삽입되는 유전자를 형질도입하고 발현시킬 수 있는 재조합 레트로바이러스 벡터는 재조합 레트로바이러스 게놈을 PA317 및 Psi-CRIP와 같은 적당한 패키지 세포주 내에 형질감염시킴으로써 제조될 수 있다[Comette et al., Human Gene Therapy 2:5-10 (1991); Cone et al., Proc. Natl. Acad. Sci. USA 81:6349 (1984)]. 재조합 아데노바이러스 벡터는 민감한 숙주(예를 들면, 래트, 햄스터, 개 및 침팬지)에서 매우 다양한 세포 및 조직을 감염시키는데 사용될 수 있으며[Hsu et al., J. Infectious Disease, 166:769 (1992)], 또한 감염을 위해 유사분열 활성 세포를 필요로 하지 않는다는 이점을 갖는다.
본 발명의 DNA 플라스미드 또는 바이러스 벡터에서 dsRNA 발현을 유도하는 프로모터는 진핵성 RNA 폴리머라제 I(예를 들면, 리보솜 RNA 프로모터), RNA 폴리머라제 II(예를 들면, CMV 초기 프로모터 또는 액틴 프로모터 또는 U1 snRNA 프로모터) 또는 바람직하게는 RNA 폴리머라제 III 프로모터(예를 들면, U6 snRNA 또는 7SK RNA 프로모터) 또는 원핵성 프로모터, 예를 들면, T7 프로모터일 수 있으나, 단, 발현 플라스미드는 또한 T7 프로모터로부터의 전사에 필요한 T7 RNA 폴리머라제를 암호화한다. 프로모터는 또한 췌장으로의 전이유전자 발현을 유도할 수 있다(예를 들면, 췌장에 대한 인슐린 조절 서열[Bucchini et al., Proc. Natl. Acad. Sci. USA 83:2511-2515 (1986)] 참조).
또한, 전이유전자의 발현은, 예를 들면, 유도성 조절 서열 및 발현 시스템, 예를 들면, 특정 생리학적 조절제, 예를 들어, 순환 글루코스 수준 또는 호르몬에 민감한 조절 서열을 사용하여 정확하게 조절될 수 있다[Docherty et al., FASEB J. 8:20-24 (1994)]. 세포 또는 포유동물에서 전이유전자 발현의 조절에 적합한 상기 유도성 발현 시스템은 엑디손, 에스트로겐, 프로게스테론, 테트라사이클린, 이량체화의 화학적 유도제, 및 이소프로필-베타-D1-티오갈락토피라노시드에 의한 조절을 포함한다. 당해 분야에 숙련된 자라면 dsRNA 전이유전자의 의도한 용도를 기준으로 적절한 조절/프로모터 서열을 선택할 수 있을 것이다.
바람직하게, dsRNA 분자를 발현할 수 있는 재조합 벡터는 하기에 기술하는 바와 같이 전달되며, 표적 세포에서 존속된다. 또는, dsRNA 분자의 일시적 발현을 제공하는 바이러스 벡터를 사용할 수 있다. 상기 벡터는 필요에 따라 반복적으로 투여될 수 있다. 일단 발현되면, dsRNA는 표적 RNA에 결합하여 그의 기능 또는 발현을 조절한다. dsRNA 발현 벡터의 전달은, 예를 들면, 정맥내 또는 근육내 투여에 의해, 환자로부터 외식된 표적 세포에 투여후 환자에 재도입함으로써, 또는 목적하는 표적 세포내로의 도입을 가능하게 하는 임의의 다른 수단에 의해 전신적으로 이루어질 수 있다.
dsRNA 발현 DNA 플라스미드는 전형적으로 양이온성 지질 담체(예를 들면, 올리고펙트아민) 또는 비-양이온성 지질-계 담체(예를 들면, 트랜지트(Transit)-TKO(등록상표))와의 복합체로서 표적 세포내에 형질감염된다. 일주일 이상의 기간에 걸쳐 단일 PTP1B 유전자 또는 다중 PTP1B 유전자의 상이한 영역을 표적으로 하는 dsRNA-매개 녹다운(knockdowun)을 위한 다중 지질 형질감염도 또한 본 발명에 포함된다. 숙주 세포내에 본 발명 벡터의 성공적인 도입은 다양한 공지된 방법을 이용하여 모니터할 수 있다. 예를 들면, 일시적 형질감염은 형광 마커, 예를 들면, 녹색 형광 단백질(GFP)과 같은 리포터를 사용하여 신호화될 수 있다. 생체외 세포의 안정한 형질감염은 특정한 환경 인자(예를 들면, 항생물질 및 약물)에 대한 저항성, 예를 들면, 하이그로마이신 B 저항성을 갖는 형질감염 세포를 제공하는 마커를 사용하여 보장될 수 있다.
하기의 상세한 설명은 dsRNA를 제조하고 사용하는 방법 및 표적 PTP1B 유전자의 발현을 억제하기 위한 dsRNA 함유 조성물, 및 상기 PTP1B 유전자의 발현에 의해 야기된 질환 및 질병을 치료하기 위한 조성물 및 방법을 개시한다.
도 1은 PTP1B dsRNA를 LNP01(1:14) 리포솜 제형으로 6 mg/kg으로 정맥내 주사후 2일째에 마우스에서 PTP1B 간 mRNA 수준에 대한 PTP1B를 표적화하는 dsRNA ("PTP1B dsRNA")의 영향을 나타낸 것이다. 루시퍼라제 dsRNA/LNP01("Luc") 및 PBS("식염수")는 대조군이다. 결과는 3 마리의 개개 동물로부터 얻은 군 평균이다.
도 2는 PTP1B dsRNA(서열번호 479/480)를 LNP01(1:14) 리포솜 제형으로 6 mg/kg으로 정맥내 주사후 마우스에서 PTP1B 간 mRNA 수준에 대한 PTP1B dsRNA의 영향에 관한 효과 연구의 기간을 나타낸 것이다. 주사후 1, 2, 3, 4, 6 및 9 일후 PTP1B 간 mRNA 수준. 루시퍼라제 dsRNA/LNP01("Luc") 및 미처리 동물은 대조군이다. 결과는 4 마리의 개개 동물로부터 얻은 군 평균이다.
도 3은 비표적 서열을 침묵시키는데 있어 서열번호 쌍 5/6을 포함하는 PTP1B dsRNA의 영향을 나타낸 것이다. 50nM PTP1B dsRNA를 사용하여, PTP1B mRNA의 19량체 표적 부위("온") 또는 인 실리코로 예측된 비표적 서열("오프 1" 내지 "오프 13"; "오프 1" - "오프 9"는 안티센스 가닥 비표적 서열이고 "오프 10" 내지 "오프13"은 센스 가닥 비표적 서열이다)을 나타내는, 이중-루시퍼라제 구조체를 발현하는 COS7 세포의 형질감염후 레닐라(renilla) 루시퍼라제 단백질의 발현. 완전히 일치하는 비표적 dsRNA는 대조군이다.
도 4는 비표적 서열을 침묵시키는데 있어 서열번호 쌍 13/14를 포함하는 PTP1B dsRNA의 영향을 나타낸 것이다. 50nM PTP1B dsRNA를 사용하여, PTP1B mRNA의 19량체 표적 부위("온") 또는 인 실리코로 예측된 비표적 서열("오프 1" 내지 "오프 13"; "오프 1" - "오프 9"는 안티센스 가닥 비표적 서열이고 "오프 10" 내지 "오프13"은 센스 가닥 비표적 서열이다)을 나타내는, 이중-루시퍼라제 구조체를 발현하는 COS7 세포의 형질감염후 레닐라 루시퍼라제 단백질의 발현. 완전히 일치하는 비표적 dsRNA는 대조군이다.
도 5는 비표적 서열을 침묵시키는데 있어 서열번호 쌍 11/12를 포함하는 PTP1B dsRNA의 영향을 나타낸 것이다. 50nM PTP1B dsRNA를 사용하여, PTP1B mRNA의 19량체 표적 부위("온") 또는 인 실리코로 예측된 비표적 서열("오프 1" 내지 "오프 17"; "오프 1" - "오프 13"는 안티센스 가닥 비표적 서열이고 "오프 14" 내지 "오프17"은 센스 가닥 비표적 서열이다)을 나타내는, 이중-루시퍼라제 구조체를 발현하는 COS7 세포의 형질감염후 레닐라 루시퍼라제 단백질의 발현. 완전히 일치하는 비표적 dsRNA는 대조군이다.
도 6은 인슐린 신호전달에 있어 dsRNA의 활성을 나타낸 것이다. HepG2 세포를 48 시간동안 5nM dsRNA로 형질감염시키고, 24 시간동안 영양제한시키고, 인슐린으로 30분간 처리한 후 패스스캔(PathScan) ELISA 분석을 행하였다. *는 모조체에 비해 상당한(p<0.05) 증가를 나타낸다. "A"는 서열번호 쌍 25/26을 포함하는 PTP1B dsRNA이고, "B"는 서열번호 쌍 11/12를 포함하는 PTP1B dsRNA이며, "C"는 서열번호 쌍 17/18을 포함하는 PTP1B dsRNA이고, "D"는 서열번호 쌍 5/6을 포함하는 PTP1B dsRNA이고, "E"는 서열번호 쌍 7/8을 포함하는 PTP1B dsRNA이며, "F"는 서열번호 쌍 3/4를 포함하는 PTP1B dsRNA이고, "G"는 서열번호 쌍 13/14를 포함하는 PTP1B dsRNA이다.
[첨부된 표에 대한 설명]
표 1은 인간 PTP1B 유전자를 표적화하는 dsRNA를 나타낸 것이다. 대문자로 나타낸 글자는 RNA 뉴클레오티드를 나타내고, 소문자 "c", "g", "a" 및 "u"는 2' O-메틸-변형된 뉴클레오티드를 나타내고, "s"는 포스포로티오에이트를, "dT"는 데옥시티미딘을 나타낸다.
표 2는 인간 PTP1B를 표적화하는 dsRNA의 특성: HepG2 및 HeLaS3 세포에서 용량 반응에 대한 활성 검사를 나타낸 것이다. IC50은 50% 억제 농도이다.
표 3은 인간 PTP1B를 표적화하는 dsRNA의 특성: 안정성 및 사이토카인 유도를 나타낸 것이다. t½은 실시예에서 정의된 바와 같은 가닥의 반감기이고, PBMC는 인간 말초혈 단핵 세포이다.
표 4는 마우스 및 래트 PTP1B 유전자를 표적화하는 dsRNA를 나타낸 것이다. 대문자로 나타낸 글자는 RNA 뉴클레오티드를 나타내고, 소문자 "c", "g", "a" 및 "u"는 2' O-메틸-변형된 뉴클레오티드를 나타내고, "s"는 포스포로티오에이트를, "dT"는 데옥시티미딘을 나타낸다. "f"는 상기 뉴클레오티드의 2' 플루오로 변형을 나타낸다.
표 5는 마우스 및 래트 PTP1B 유전자를 표적화하는 dsRNA의 특성: 안정성 및 사이토카인 유도를 나타낸 것이다. t½은 실시예에서 정의된 바와 같은 가닥의 반감기이고, PBMC는 인간 말초혈 단핵 세포이다.
표 6은 서열번호 쌍 5/6을 포함하는, 인간 PTP1B를 표적화하는 dsRNA의 선택된 비표적 서열이다.
표 7은 서열번호 쌍 13/14를 포함하는, 인간 PTP1B를 표적화하는 dsRNA의 선택된 비표적 서열이다.
표 8은 서열번호 쌍 11/12를 포함하는 인간 PTP1B를 표적화하는 dsRNA의 선택된 비표적 서열이다.
표 9는 인간 PTP1B의 측정을 위한 bDNA 프로브의 서열이며; LE는 표지 확장자, CE는 캡처 확장자, BL은 차단 프로브이다.
표 10은 인간 GAPDH의 측정을 위한 bDNA 프로브의 서열이며; LE는 표지 확장자, CE는 캡처 확장자, BL은 차단 프로브이다.
표 11은 마우스/래트 PTP1B의 측정을 위한 bDNA 프로브의 서열이며; LE는 표지 확장자, CE는 캡처 확장자, BL은 차단 프로브이다.
표 12는 마우스/래트 GAPDH의 측정을 위한 bDNA 프로브의 서열이며; LE는 표지 확장자, CE는 캡처 확장자, BL은 차단 프로브이다.
표 13은 인간 PTP1B 유전자를 표적화하는 dsRNA를 나타낸 것이다. 대문자로 나타낸 글자는 RNA 뉴클레오티드를 나타낸다.
표 14는 변형 및 그의 변형된 대응물을 갖지 않는 인간 PTP1B 유전자를 표적화하는 dsRNA를 나타낸 것이다. 대문자로 나타낸 글자는 RNA 뉴클레오티드를 나타내고, 소문자 "c", "g", "a" 및 "u"는 2' O-메틸-변형된 뉴클레오티드를 나타내고, "s"는 포스포로티오에이트를, "dT"는 데옥시티미딘을 나타낸다.
도 2는 PTP1B dsRNA(서열번호 479/480)를 LNP01(1:14) 리포솜 제형으로 6 mg/kg으로 정맥내 주사후 마우스에서 PTP1B 간 mRNA 수준에 대한 PTP1B dsRNA의 영향에 관한 효과 연구의 기간을 나타낸 것이다. 주사후 1, 2, 3, 4, 6 및 9 일후 PTP1B 간 mRNA 수준. 루시퍼라제 dsRNA/LNP01("Luc") 및 미처리 동물은 대조군이다. 결과는 4 마리의 개개 동물로부터 얻은 군 평균이다.
도 3은 비표적 서열을 침묵시키는데 있어 서열번호 쌍 5/6을 포함하는 PTP1B dsRNA의 영향을 나타낸 것이다. 50nM PTP1B dsRNA를 사용하여, PTP1B mRNA의 19량체 표적 부위("온") 또는 인 실리코로 예측된 비표적 서열("오프 1" 내지 "오프 13"; "오프 1" - "오프 9"는 안티센스 가닥 비표적 서열이고 "오프 10" 내지 "오프13"은 센스 가닥 비표적 서열이다)을 나타내는, 이중-루시퍼라제 구조체를 발현하는 COS7 세포의 형질감염후 레닐라(renilla) 루시퍼라제 단백질의 발현. 완전히 일치하는 비표적 dsRNA는 대조군이다.
도 4는 비표적 서열을 침묵시키는데 있어 서열번호 쌍 13/14를 포함하는 PTP1B dsRNA의 영향을 나타낸 것이다. 50nM PTP1B dsRNA를 사용하여, PTP1B mRNA의 19량체 표적 부위("온") 또는 인 실리코로 예측된 비표적 서열("오프 1" 내지 "오프 13"; "오프 1" - "오프 9"는 안티센스 가닥 비표적 서열이고 "오프 10" 내지 "오프13"은 센스 가닥 비표적 서열이다)을 나타내는, 이중-루시퍼라제 구조체를 발현하는 COS7 세포의 형질감염후 레닐라 루시퍼라제 단백질의 발현. 완전히 일치하는 비표적 dsRNA는 대조군이다.
도 5는 비표적 서열을 침묵시키는데 있어 서열번호 쌍 11/12를 포함하는 PTP1B dsRNA의 영향을 나타낸 것이다. 50nM PTP1B dsRNA를 사용하여, PTP1B mRNA의 19량체 표적 부위("온") 또는 인 실리코로 예측된 비표적 서열("오프 1" 내지 "오프 17"; "오프 1" - "오프 13"는 안티센스 가닥 비표적 서열이고 "오프 14" 내지 "오프17"은 센스 가닥 비표적 서열이다)을 나타내는, 이중-루시퍼라제 구조체를 발현하는 COS7 세포의 형질감염후 레닐라 루시퍼라제 단백질의 발현. 완전히 일치하는 비표적 dsRNA는 대조군이다.
도 6은 인슐린 신호전달에 있어 dsRNA의 활성을 나타낸 것이다. HepG2 세포를 48 시간동안 5nM dsRNA로 형질감염시키고, 24 시간동안 영양제한시키고, 인슐린으로 30분간 처리한 후 패스스캔(PathScan) ELISA 분석을 행하였다. *는 모조체에 비해 상당한(p<0.05) 증가를 나타낸다. "A"는 서열번호 쌍 25/26을 포함하는 PTP1B dsRNA이고, "B"는 서열번호 쌍 11/12를 포함하는 PTP1B dsRNA이며, "C"는 서열번호 쌍 17/18을 포함하는 PTP1B dsRNA이고, "D"는 서열번호 쌍 5/6을 포함하는 PTP1B dsRNA이고, "E"는 서열번호 쌍 7/8을 포함하는 PTP1B dsRNA이며, "F"는 서열번호 쌍 3/4를 포함하는 PTP1B dsRNA이고, "G"는 서열번호 쌍 13/14를 포함하는 PTP1B dsRNA이다.
[첨부된 표에 대한 설명]
표 1은 인간 PTP1B 유전자를 표적화하는 dsRNA를 나타낸 것이다. 대문자로 나타낸 글자는 RNA 뉴클레오티드를 나타내고, 소문자 "c", "g", "a" 및 "u"는 2' O-메틸-변형된 뉴클레오티드를 나타내고, "s"는 포스포로티오에이트를, "dT"는 데옥시티미딘을 나타낸다.
표 2는 인간 PTP1B를 표적화하는 dsRNA의 특성: HepG2 및 HeLaS3 세포에서 용량 반응에 대한 활성 검사를 나타낸 것이다. IC50은 50% 억제 농도이다.
표 3은 인간 PTP1B를 표적화하는 dsRNA의 특성: 안정성 및 사이토카인 유도를 나타낸 것이다. t½은 실시예에서 정의된 바와 같은 가닥의 반감기이고, PBMC는 인간 말초혈 단핵 세포이다.
표 4는 마우스 및 래트 PTP1B 유전자를 표적화하는 dsRNA를 나타낸 것이다. 대문자로 나타낸 글자는 RNA 뉴클레오티드를 나타내고, 소문자 "c", "g", "a" 및 "u"는 2' O-메틸-변형된 뉴클레오티드를 나타내고, "s"는 포스포로티오에이트를, "dT"는 데옥시티미딘을 나타낸다. "f"는 상기 뉴클레오티드의 2' 플루오로 변형을 나타낸다.
표 5는 마우스 및 래트 PTP1B 유전자를 표적화하는 dsRNA의 특성: 안정성 및 사이토카인 유도를 나타낸 것이다. t½은 실시예에서 정의된 바와 같은 가닥의 반감기이고, PBMC는 인간 말초혈 단핵 세포이다.
표 6은 서열번호 쌍 5/6을 포함하는, 인간 PTP1B를 표적화하는 dsRNA의 선택된 비표적 서열이다.
표 7은 서열번호 쌍 13/14를 포함하는, 인간 PTP1B를 표적화하는 dsRNA의 선택된 비표적 서열이다.
표 8은 서열번호 쌍 11/12를 포함하는 인간 PTP1B를 표적화하는 dsRNA의 선택된 비표적 서열이다.
표 9는 인간 PTP1B의 측정을 위한 bDNA 프로브의 서열이며; LE는 표지 확장자, CE는 캡처 확장자, BL은 차단 프로브이다.
표 10은 인간 GAPDH의 측정을 위한 bDNA 프로브의 서열이며; LE는 표지 확장자, CE는 캡처 확장자, BL은 차단 프로브이다.
표 11은 마우스/래트 PTP1B의 측정을 위한 bDNA 프로브의 서열이며; LE는 표지 확장자, CE는 캡처 확장자, BL은 차단 프로브이다.
표 12는 마우스/래트 GAPDH의 측정을 위한 bDNA 프로브의 서열이며; LE는 표지 확장자, CE는 캡처 확장자, BL은 차단 프로브이다.
표 13은 인간 PTP1B 유전자를 표적화하는 dsRNA를 나타낸 것이다. 대문자로 나타낸 글자는 RNA 뉴클레오티드를 나타낸다.
표 14는 변형 및 그의 변형된 대응물을 갖지 않는 인간 PTP1B 유전자를 표적화하는 dsRNA를 나타낸 것이다. 대문자로 나타낸 글자는 RNA 뉴클레오티드를 나타내고, 소문자 "c", "g", "a" 및 "u"는 2' O-메틸-변형된 뉴클레오티드를 나타내고, "s"는 포스포로티오에이트를, "dT"는 데옥시티미딘을 나타낸다.
정의
편의상, 명세서, 실시예 및 첨부된 특허청구범위에 사용된 특정 용어 및 어구의 의미를 하기에 제공한다. 본 명세서의 다른 부분에서의 용어 사용과 이 부분에 제공된 그의 정의 사이에 명백한 불일치가 존재하는 경우, 이 부분에 나타낸 정의가 우선된다.
"G", "C", "A", "U" 및 "T" 또는 "dT"는 각각 일반적으로 염기로서 구아닌, 시토신, 아데닌, 우라실 및 데옥시티미딘을 각각 함유하는 뉴클레오티드를 의미한다. 그러나, 용어 "리보뉴클레오티드" 또는 "뉴클레오티드"는 또한 하기에서 더 상술하는 바와 같이 변형된 뉴클레오티드, 또는 대리 치환 잔기를 말할 수 있다. 상기 치환 잔기를 포함하는 서열은 본 발명의 태양을 이룬다. 하기에서 상술하는 바와 같이, 본원에 기술된 dsRNA 분자는 또한 "돌출부(overhang)", 즉, 본원에 정의된 "센스 가닥" 및 "안티센스 가닥"의 쌍에 의해 통상적으로 형성되는 RNA 이중 나선 구조에 직접 포함되지 않는, 쌍을 이루지 않은 돌출된 뉴클레오티드를 포함할 수 있다. 흔히, 상기 돌출된 연장부는 3' 말단에 데옥시티미딘 뉴클레오티드, 대부분의 태양에서 2-데옥시티미딘을 포함한다. 상기 돌출부는 하기에서 설명하고 예시될 것이다.
본원에서 사용된 바와 같이, 용어 "PTP1B"는 특히 PTPN1으로도 알려져 있는 단백질 포스파타제 1B에 관한 것이며, 상기 용어는 상응하는 유전자, 암호화된 mRNA, 암호화된 단백질/폴리펩티드, 및 이들의 기능적 단편들에 관한 것이다. 인간 PTP1B 유전자가 바람직하다. 다른 바람직한 태양에서, 본 발명의 dsRNA는 래트(래터스 노르베기커스) 및 마우스(무스 무스큘러스)의 PTP1B 유전자를 표적으로 하며, 또 다른 바람직한 태양에서, 본 발명의 dsRNA는 인간(호모 사피엔스(H. sapiens)) 및 사이노몰구스(cynomolgous) 원숭이(마카카 파시큘라리스(Macaca fascicularis)) PTP1B 유전자를 표적으로 한다. 용어 "PTP1B 유전자/서열"은 야생형 서열(들)에만 관한 것이 아니라 상기 유전자/서열에 포함될 수 있는 돌연변이 및 변형에도 관한 것이다. 따라서, 본 발명은 본원에 제공된 특정 dsRNA 분자로 한정되지 않는다. 본 발명은 또한 상기 돌연변이/변형을 포함하는 PTP1B 유전자의 RNA 전사체의 상응하는 뉴클레오티드 연장부에 85% 이상 상보성인 안티센스 가닥을 포함하는 dsRNA 분자에 관한 것이다.
본원에서 사용된 바와 같이, "표적 서열"은 1차 전사 생성물의 RNA 처리과정의 산물인 mRNA를 포함하여, PTP1B 유전자의 전사동안 생성된 mRNA 분자의 뉴클레오티드 서열의 인접 부분을 말한다.
본원에서 사용된 바와 같이, 용어 "서열을 포함하는 가닥"은 표준 뉴클레오티드 명명법을 사용하여 지칭된 서열에 의해 설명되는 뉴클레오티드 쇄를 포함하는 올리고뉴클레오티드를 말한다. 그러나, 본원에서 상술한 바와 같이, 상기 "서열을 포함하는 가닥"은 또한 변형된 뉴클레오티드와 같이 변형을 포함할 수 있다.
본 원에서 사용된 바와 같이, 달리 언급하지 않는 한, 용어 "상보성"은, 제 2의 뉴클레오티드 서열에 관하여 제 1 뉴클레오티드 서열을 설명하기 위해 사용될 때, 특정 조건하에서 제 2 뉴클레오티드 서열을 포함하는 올리고뉴클레오티드 또는 폴리뉴클레오티드와 하이브리드화되어 이중 구조를 형성하는, 제 1 뉴클레오티드 서열을 포함하는 올리고뉴클레오티드 또는 폴리뉴클레오티드의 능력을 말한다. 본원에서 사용된 바와 같이, "상보성" 서열은 또한, 하이브리드화되는 그의 능력에 관한 상기 필요조건들이 달성되는 한에 있어서는, 비-왓슨-크릭(Watson-Crick) 염기쌍 및/또는 비-천연 및 변형 뉴클레오티드로부터 생성된 염기쌍을 포함할 수 있거나 또는 전적으로 이들로부터 생성될 수 있다.
"완전히 상보성"으로 언급된 서열은 제 1 및 제 2 뉴클레오티드 서열의 전체 길이에 걸쳐 제 2 뉴클레오티드 서열을 포함하는 올리고뉴클레오티드 또는 폴리뉴클레오티드에 대한 제 1 뉴클레오티드 서열을 포함하는 올리고뉴클레오티드 또는 폴리뉴클레오티드의 염기-쌍을 포함한다.
그러나, 제 1 서열이 본원의 제 2 서열에 대해 "실질적으로 상보성"으로 언급되는 경우, 두 서열은 완전히 상보성일 수 있거나, 또는 이들은 하이브리드화될 때 하나 이상, 바람직하게는 13개 이하의 일치하지 않는 염기쌍을 형성할 수 있다.
본원에서 용어 "상보성", "완전히 상보성" 및 "실질적으로 상보성"은, 그 사용의 맥락에서 이해되듯이, dsRAN의 센스 가닥과 안티센스 가닥 사이, 또는 dsRNA의 안티센스 가닥과 표적 서열 사이에 일치하는 염기에 대해 사용될 수 있다.
본원에서 사용된 바와 같이, 용어 "이중-가닥 RNA", "dsRNA 분자" 또는 "dsRNA"는 2개의 역평행 및 실질적으로 상보성인 핵산 가닥을 포함하는 이중 구조를 갖는 리보핵산 분자 또는 리보핵산 분자의 복합체를 말한다. 이중 구조를 형성하는 2개의 가닥은 하나의 큰 RNA 분자의 상이한 부분들일 수 있거나, 또는 이들은 별개의 RNA 분자일 수 있다. 2개의 가닥이 하나의 큰 분자의 일부이고 따라서 한 가닥의 3'-말단과 이중 구조를 형성하는 각각의 다른 가닥의 5' 말단 사이에 뉴클레오티드의 중단되지 않은 쇄에 의해 연결되는 경우, 연결하는 RNA 쇄를 "머리핀 루프"로 칭한다. 2개의 가닥이 한 가닥의 3'-말단과 이중 구조를 형성하는 각각의 다른 가닥의 5' 말단 사이에 뉴클레오티드의 중단되지 않은 쇄 이외의 다른 수단에 의해 공유적으로 연결되는 경우, 연결하는 구조를 "링커"로 칭한다. RNA 가닥은 같거나 다른 수의 뉴클레오티드를 가질 수 있다. 이중 구조 이외에, dsRNA는 하나 이상의 뉴클레오티드 돌출부를 포함할 수 있다. 상기 "돌출부"에서 뉴클레오티드는 0 내지 5개의 뉴클레오티드를 포함할 수 있으며, 이때 "0"은 "돌출부"를 형성하는 추가의 뉴클레오티드(들)이 없음을 의미하는 반면, "5"는 dsRNA 이중체의 개개 가닥 상에 5개의 추가의 뉴클레오티드를 의미한다. 상기 임의의 "돌출부"는 개개 가닥의 3'-말단에 위치한다. 하기에서 상술하는 바와 같이, 두 가닥 중 하나에만 "돌출부"를 포함하는 dsRNA 분자도 또한 본 발명에 있어 유용하고 심지어 유리할 수 있다. "돌출부"는 바람직하게는 0 내지 2개의 뉴클레오티드를 포함한다. 가장 바람직하게는, 2개의 "dT"(데옥시티미딘) 뉴클레오티드가 dsRNA의 두 가닥 모두의 3' 말단에서 발견된다. 또한, 2개의 "U"(우라실) 뉴클레오티드도 dsRNA의 양쪽 가닥의 3' 말단에 돌출부로 사용될 수 있다. 따라서, "뉴클레오티드 돌출부"는, dsRNA의 한쪽 가닥의 3'-말단이 다른 가닥의 5'-말단을 지나 연장되거나 또는 그 반대인 경우에, dsRNA의 이중 구조로부터 돌출되는, 쌍을 이루지 않는 뉴클레오티드 또는 뉴클레오티드들을 말한다. 예를 들면, 안티센스 가닥은 23개의 뉴클레오티드를 포함하고 센스 가닥은 21개의 뉴클레오티드를 포함하면, 안티센스 가닥의 3' 말단에 2개 뉴클레오티드 돌출부를 형성한다. 바람직하게는, 2개 뉴클레오티드 돌출부는 표적 유전자의 mRNA에 완전히 상보성이다. "평활부" 또는 "평활 말단"은 dsRNA의 말단에 쌍을 이루지 않은 뉴클레오티드가 없음을, 즉, 뉴클레오티드 돌출부가 없음을 의미한다. "평활 말단" dsRNA는 그 전체 길이에 걸쳐 이중-가닥인, 즉, 분자의 어느 말단에도 뉴클레오티드 돌출부가 없는 dsRNA이다.
용어 "안티센스 가닥"은 표적 서열에 실질적으로 상보성인 영역을 포함하는 dsRNA의 가닥을 말한다. 본원에서 사용된 바와 같이, 용어 "상보성 영역"은 서열, 예를 들면, 표적 서열에 실질적으로 상보성인 안티센스 가닥 상의 영역을 말한다. 상보성 영역이 표적 서열에 완전히 상보성이 아닌 경우, 미스매치(mismatch)는 안티센스 가닥의 5' 말단의 뉴클레오티드 2 내지 7 이외에서 가장 잘 허용된다.
본원에서 사용된 바와 같이, 용어 "센스 가닥"은 안티센스 가닥의 영역에 실질적으로 상보성인 영역을 포함하는 dsRNA의 가닥을 말한다. "실질적으로 상보성"은 바람직하게는 센스 및 안티센스 가닥에서 중복되는 뉴클레오티드의 85% 이상이 상보성인 것을 의미한다.
"세포내로의 도입"은, dsRNA를 언급하는 경우, 당해 분야에 숙련된 자에 의해 이해되듯이, 세포내에 흡수 또는 통합을 촉진하는 것을 의미한다. dsRNA의 통합 또는 흡수는 비보조 확산 또는 활성 세포 과정을 통해, 또는 보조제 또는 보조 장치에 의해 일어날 수 있다. 상기 용어의 의미는 시험관내에서 세포로 제한되지 않으며; dsRNA는 또한 "세포내로의 도입"될 수 있는데, 이때 세포는 살아있는 유기체의 일부이다. 상기 경우에, 세포내로의 도입은 유기체로의 전달을 포함한다. 예를 들면, 생체내 전달의 경우, dsRNA는 조직 부위 내에 주사되거나 또는 전신적으로 투여될 수 있다. 예를 들면, 본 발명의 dsRNA 분자를 의학적 개입이 필요한 대상에게 투여하는 것이 고려된다. 상기 투여는 본 발명의 dsRNA, 벡터 또는 세포를 상기 대상에서 질환이 있는 부위, 예를 들면, 간 조직/세포 또는 간암 조직과 같은 암 조직/세포 내에 주사하는 것을 포함할 수 있다. 그러나, 질환 조직의 매우 근접한 부위에 주사하는 것도 또한 고려된다. 시험관내에서, 세포내로의 도입은 전기천공 및 리포펙션(lipofection)과 같이 당해 분야에 공지된 방법을 포함한다.
용어 "침묵", "발현 억제" 및 "녹다운"은 본원에서, PTP1B 유전자를 지칭하는 한에 있어서는, 첫 번째 세포 또는 세포 군과 실질적으로 동일하지만 PTP1B 유전자 발현이 억제되도록 처리되지 않은 두 번째 세포 또는 세포 군(대조군 세포)에 비해, PTP1B 유전자가 전사되고 PTP1B 유전자의 발현이 억제되도록 처리된 첫 번째 세포 또는 세포 군으로부터 분리될 수 있는 PTP1B 유전자로부터 전사된 mRNA의 양의 감소에 의해 평가되듯이, PTP1B 유전자 발현의 적어도 부분적 억제를 말한다. 억제 정도는 통상적으로 다음으로 나타낸다:
[(대조군 세포의 mRNA)-(처리된 세포의 mRNA)]/(대조군 세포의 mRNA) x 100%
또는, 억제 정도는 PTP1B 유전자 전사에 기능적으로 관련되는 파라미터의 감소, 예를 들면, 세포에 의해 분비되는 PTP1B 유전자에 의해 암호화된 단백질의 양, 또는 특정 표현형을 나타내는 세포의 수의 감소로 나타낼 수 있다.
본원에 제공된 첨부된 실시예 및 첨부된 표에 예시된 바와 같이, 본 발명의 dsRNA 분자는 시험관내 분석에서, 즉, 시험관내에서, 인간 PTP1B의 발현을 약 60% 이상, 바람직하게는 70% 이상, 가장 바람직하게는 80% 이상까지 억제할 수 있다. 본원에서 사용된 바와 같이 용어 "시험관내에서"는 세포 배양물 분석을 포함하나 이로 제한되는 것은 아니다. 또 다른 태양에서, 본 발명의 dsRNA 분자는 마우스 또는 래트 PTP1B의 발현을 60% 이상, 바람직하게는 70% 이상, 가장 바람직하게는 80% 이상까지 억제할 수 있다. 당해 분야에 숙련된 자라면 특히 본원에 제공된 분석을 고려하여 상기 억제율 및 관련된 결과들을 용이하게 결정할 수 있다.
본원에서 사용된 바와 같이, 용어 "비표적(off target)"은 서열 상보성을 기준으로, 기술된 dsRNA에 하이브리드화시키기 위한 인 실리코(in silico) 방법에 의해 예상되는 전사체의 모든 비-표적 mRNA를 말한다. 본 발명의 dsRNA는 바람직하게는 PTP1B의 발현을 특이적으로 억제한다, 즉, 임의의 비표적의 발현은 억제하지 않는다.
본원에서 사용된 바와 같이 용어 "반감기"는 화합물 또는 분자의 안정성의 척도이며, 특히 본원에 제공된 분석을 고려하여 당해 분야에 숙련된 자에게 공지된 방법에 의해 평가할 수 있다.
본원에서 사용된 바와 같이 용어 "비-면역조절성"은 본 발명의 dsRNA 분자에 의한 면역 반응의 임의의 유도의 부재를 말한다. 면역 반응을 측정하는 방법은, 예를 들면, 실시예 부분에 기술된 바와 같이, 사이토카인의 방출을 측정함으로써, 당해 분야에 숙련된 자에게 공지되어 있다.
용어 "치료하다", "치료" 등은, 본 발명의 맥락에서, 2형 당뇨병, 비만, 간부전, 이상지질혈증, 당뇨병성 죽상동맥경화증 및 고혈압과 같이 PTP1B 발현과 관련된 질병의 경감 또는 완화를 의미한다. 본원에서 사용된 바와 같이 용어 "간부전"은 간이 그 기능을 다하지 못하거나 간에 부과되는 요구를 충족시킬 수 없는 모든 상태를 말한다. 간부전은, 예를 들면, 트라우마, 종양성 침습, 장기적 담도 폐쇄, 바이러스 감염(예를 들면, C형 간염) 또는 만성 알콜중독의 결과로 일어날 수 있다.
본원에서 사용된 바와 같이, "약학 조성물"은 약리학적으로 효과량의 dsRNA 및 약학적으로 허용되는 담체를 포함한다. 그러나, 상기 "약학 조성물"은 또한 상기 dsRNA 분자의 개개 가닥을 포함하거나, 또는 본 발명의 dsRNA에 포함되는 센스 또는 안티센스 가닥 중 하나 이상의 가닥을 암호화하는 뉴클레오티드 서열에 작동가능하게 연결된 조절 서열을 포함하는 본원에 기술된 벡터(들)을 포함할 수 있다. 본원에 정의된 dsRNA를 발현하거나 이를 포함하는 세포, 조직 또는 분리된 장기를 "약학 조성물"로 사용할 수 있는 것으로 또한 생각된다. 본원에서 사용된 바와 같이, "약리학적 효과량", "치료 효과량" 또는 간단히 "효과량"은 의도한 약리학적, 치료적 또는 예방적 결과를 제공하기에 효과적인 RNA의 양을 말한다.
용어 "약학적으로 허용되는 담체"는 치료제의 투여를 위한 담체를 말한다. 상기 담체로는 식염수, 완충 식염수, 덱스트로스, 물, 글리세롤, 에탄올 및 그의 혼합물이 포함되나, 이로 한정되지는 않는다. 상기 용어는 특별히 세포 배양 배지를 배제한다. 경구 투여되는 약물의 경우, 약학적으로 허용되는 담체로는 약학적으로 허용되는 부형제, 예를 들면, 당해 분야에 숙련된 자에게 공지된 바와 같은 불활성 희석제, 붕해제, 결합제, 윤활제, 감미제, 향미제, 착색제 및 방부제가 포함되나, 이로 한정되지는 않는다.
특히, 약학적으로 허용되는 담체는 본 발명의 dsRNA, 벡터 또는 세포의 전신 투여를 가능하게 하는 것으로 생각된다. 또한 장내 투여도 고려되는 한편, 비경구 투여 및 또한 경피 또는 경점막(transmucosal)(예를 들면, 흡입, 구강내, 질내, 항문내) 투여 뿐 아니라 약물의 흡입은 의학적 개입이 필요한 환자에게 본 발명의 화합물을 투여하는 가능한 방법이다. 비경구 투여를 이용하는 경우, 상기 투여는 본 발명의 화합물을 질환 조직내에 또는 적어도 아주 근접하여 직접 주사하는 것을 포함한다. 그러나, 본 발명 화합물의 정맥내, 동맥내, 피하, 근육내, 복강내, 피내, 척추강내 및 기타 투여방법도 전문가, 예를 들면, 주치의의 기량에 포함된다.
근육내, 피하 및 정맥내 사용의 경우, 본 발명의 약학 조성물은 일반적으로 적절한 pH 및 등장성으로 완충된 멸균 수용액 또는 현탁액으로 제공될 것이다. 바람직한 태양에서, 담체는 오직 수성 완충제로 이루어진다. 이와 관련하여, "오직"은 PTP1B 유전자를 발현하는 세포에서 dsRNA의 흡수에 작용하거나 영향을 줄 수 있는 어떤 보조제 또는 캡슐화 물질도 존재하지 않음을 의미한다. 본 발명에 따른 수성 현탁액은 현탁제, 예를 들면, 셀룰로스 유도체, 나트륨 알기네이트, 폴리비닐-피롤리돈 및 트라가칸트 고무, 및 레시틴과 같은 습윤제를 포함할 수 있다. 수성 현탁액에 적당한 방부제로는 에틸 및 n-프로필 p-하이드록시벤조에이트가 포함된다. 본 발명에 따라 유용한 약학 조성물은 또한 dsRNA가 신체로부터 신속히 제거되는 것을 방지하기 위한 캡슐화 제형, 예를 들면, 이식물 및 미세캡슐화 전달 시스템을 포함하여 방출 조절 제형을 포함한다. 생분해성, 생체적합성 중합체, 예를 들면, 에틸렌 비닐 아세테이트, 폴리무수물, 폴리글라이콜산, 콜라겐, 폴리오쏘에스터 및 폴리락트산을 사용할 수 있다. 상기 제형의 제조 방법은 당해 분야에 숙련된 자에게 명백할 것이다. 리포솜 현탁액도 또한 약학적으로 허용되는 담체로 사용될 수 있다. 이들은 당해 분야에 숙련된 자에게 공지된 방법에 따라, 예를 들면, 본원에 참고로 인용된 PCT 공개공보 WO 91/06309 호에 기술된 바와 같이 제조할 수 있다.
본원에서 사용된 바와 같이, "형질감염 세포"는 그로부터 dsRAN 분자 또는 상기 dsRNA 분자의 하나 이상의 가닥이 발현될 수 있도록 하나 이상의 벡터가 그 안에 도입된 세포이다. 상기 벡터는 바람직하게는 본 발명의 dsRNA에 포함되는 센스 가닥 또는 안티센스 가닥 중 하나 이상을 암호화하는 뉴클레오티드 서열에 작동가능하게 연결된 조절 서열을 포함하는 벡터이다.
한쪽 또는 양쪽 말단상에 단지 소수의 뉴클레오티드를 제외한 표 1 및 4의 서열들중 하나를 포함하는 보다 짧은 dsRNA도 전술한 dsRNA와 비교할 때 유사하게 효과적일 수 있을 것으로 타당하게 예상할 수 있다. 상기에서 지적한 바와 같이, 본 발명의 대부분의 태양에서, 본원에 제공된 dsRNA 분자는 약 16 내지 약 30개 뉴클레오티드의 이중 길이(즉, "돌출부" 없이)를 포함한다. 특히 유용한 dsRNA 이중 길이는 약 19 내지 약 25개 뉴클레오티드이다. 19개 뉴클레오티드의 길이를 갖는 이중 구조가 가장 바람직하다. 본 발명의 dsRNA 분자에서, 안티센스 가닥은 센스 가닥에 적어도 부분적으로 상보성이다.
한 바람직한 태양에서, 본 발명의 dsRNA 분자는 표 13에 나타낸 서열의 뉴클레오티드 1 내지 19개를 포함한다.
본 발명의 dsRNA는 표적 서열에 대한 하나 이상의 미스매치를 함유할 수 있다. 바람직한 태양에서, 본 발명의 dsRNA는 13개 이하의 미스매치를 함유한다. dsRNA의 안티센스 가닥이 표적 서열에 대해 미스매치를 함유하는 경우, 미스매치 영역은 안티센스 가닥의 5' 말단의 뉴클레오티드 2 내지 7 내에 위치하지 않는 것이 바람직하다. 또 다른 태양에서, 미스매치 영역은 안티센스 가닥의 5' 말단의 뉴클레오티드 2 내지 9 내에 위치하지 않는 것이 바람직하다.
상기 언급한 바와 같이, dsRNAd의 하나 이상의 말단/가닥은 1 내지 5개, 바람직하게는 1 또는 2개의 뉴클레오티드의 단일-가닥 뉴클레오티드 돌출부를 가질 수 있다. 하나 이상의 뉴클레오티드 돌출부를 갖는 dsRNA는 예상외로 그의 평활-말단 대응물보다 월등한 억제 특성을 갖는다. 게다가, 본 발명자들은 단지 하나의 뉴클레오티드 돌출부의 존재가 그 전체 안정성에 영향을 미치지 않고 dsRNA의 간섭 활성을 강화시킴을 알아내었다. 단지 하나의 돌출부를 갖는 dsRNA는 생체내에서 뿐 아니라, 다양한 세포, 세포 배양 배지, 혈액 및 혈청에서 특히 안정하고 효과적인 것으로 입증되었다. 바람직하게, 단일-가닥 돌출부는 안티센스 가닥의 3'-말단부에 또는 센스 가닥의 3'-말단부에 위치한다. dsRNA는 또한 바람직하게는 안티센스 가닥의 5'-말단에 위치한 평활 말단을 가질 수 있다. 바람직하게, dsRNA의 안티센스 가닥은 3'-말단에 뉴클레오티드 돌출부를 가지며, 5'-말단은 평활하다. 또 다른 태양에서, 돌출부의 하나 이상의 뉴클레오티드는 뉴클레오시드 티오포스페이트로 대체된다.
본 발명의 dsRNA는 또한 안정성을 증대시키기 위해 화학적으로 변형될 수 있다. 본 발명의 핵산은 당해 분야에 잘 확립된 방법에 의해, 예를 들면, 본원에 참고로 인용된 문헌 ["Current protocols in nucleic acid chemistry", Beaucage, S.L. et al.(Edrs.), John Wiley & Sons, Inc., New York, NY, USA]에 기술된 방법에 의해 합성되고/되거나 변형될 수 있다. 화학적 변형은 2' 변형, 비-천연 염기의 도입, 리간드에 공유적 부착, 및 티오포스페이트 결합에 의한 포스페이트 결합의 치환을 포함할 수 있으나, 이로 한정되지는 않는다. 상기 태양에서, 이중 구조의 보전성은 하나 이상, 바람직하게는 2개의 화학적 결합에 의해 강화된다. 화학적 결합은 임의의 다양한 공지된 기술에 의해, 예를 들면, 공유, 이온성 또는 수소 결합; 소수성 상호작용, 반 데르 발스(van der Waals) 또는 적층결합(stacking) 상호작용을 도입함으로써; 금속-이온 배위에 의해, 또는 퓨린 유사체의 사용을 통해 달성될 수 있다. 바람직하게는, dsRNA를 변형하기 위해 사용될 수 있는 화학적 기로는, 제한하지 않고, 메틸렌 블루; 이작용성 기, 바람직하게는 비스-(2-클로로에틸)아민; N-아세틸-N'-(p-글라이옥실벤조일)시스타민; 4-티오우라실; 및 소랄렌이 포함된다. 하나의 바람직한 태양에서, 링커는 헥사-에틸렌 글라이콜 링커이다. 이 경우에, dsRNA는 고체상 합성에 의해 생성되며, 헥사-에틸렌 글라이콜 링커는 표준 방법에 따라 혼입된다(예를 들면, 문헌[Williams, D.J., and K.B. Hall, Biochem. 35:14665-14670 (1996)] 참조). 특정 태양에서, 안티센스 가닥의 5'-말단 및 센스 가닥의 3'-말단은 헥사에틸렌 글라이콜 링커에 의해 화학적으로 연결된다. 또 다른 태양에서, dsRNA의 하나 이상의 뉴클레오티드는 포스포로티오에이트 또는 포스포로다이티오에이트 기를 포함한다. dsRNA의 말단에서의 화학적 결합은 바람직하게는 삼중-나선 결합에 의해 형성된다.
특정 태양에서, 화학적 결합은 하나 또는 여러개의 결합 기에 의해 형성될 수 있으며, 이때 상기 결합 기는 바람직하게는 폴리-(옥시포스피니코옥시-1,3-프로판다이올)- 및/또는 폴리에틸렌 글라이콜 쇄이다. 다른 태양에서, 화학적 결합은 또한 퓨린 대신에 이중-가닥 구조내에 도입된 퓨린 유사체에 의해 형성될 수 있다. 또 다른 태양에서, 화학적 결합은 이중-가닥 구조내에 도입된 아자벤젠 단위에 의해 형성될 수 있다. 또 다른 태양에서, 화학적 결합은 이중-가닥 구조내에 도입된 뉴클레오티드 대신에 가교된 뉴클레오티드 유사체에 의해 형성될 수 있다. 특정 태양에서, 화학적 결합은 자외선에 의해 유도될 수 있다.
또 다른 태양에서, 두 단일 가닥중 하나 또는 둘 다에서 뉴클레오티드는 세포성 효소, 예를 들면, 특정 뉴클레아제의 활성화를 방지 또는 억제하기 위해 변형될 수 있다. 세포성 효소의 활성화를 억제하기 위한 기술은, 2'-아미노 변형, 2'-아미노 당 변형, 2'-F 당 변형, 2'-F 변형, 2'-알킬 당 변형, 비하전 주쇄 변형, 모르폴리노 변형, 2'-O-메틸 변형 및 포스포르아미데이트를 포함하여(이로 한정되지는 않는다), 당해 분야에 공지되어 있다(예를 들면, 문헌 [Wagner, Nat. Med. 1:1116-1118 (1995)] 참조). 따라서, dsRNA 상의 뉴클레오티드의 하나 이상의 2'-하이드록실기는 화학적 기에 의해, 바람직하게는 2'-아미노 또는 2'-메틸기에 의해 치환된다. 또한, 하나 이상의 뉴클레오티드는 변형되어 고정 뉴클레오티드를 형성할 수 있다. 상기 고정 뉴클레오티드는 리보스의 2'-산소를 리보스의 4'-탄소와 연결시키는 메틸렌 가교를 함유한다. 올리고뉴클레오티드 내에 고정 뉴클레오티드의 도입은 상보성 서열에 대한 친화도를 개선시키고 용융 온도를 수도까지 증가시킨다.
본원에 제공된 dsRNA 분자의 변형은 생체내 및 시험관내에서 그의 안정성에 유리하게 영향을 미칠 수 있으며 또한 (질환이 있는) 표적 면으로의 그의 전달을 개선시킬 수 있다. 또한, 상기 구조적 및 화학적 변형은 투여시 dsRNA 분자에 대한 생리학적 반응, 예를 들면, 억제되는 것이 바람직한 사이토카인 방출에 유리하게 영향을 미칠 수 있다. 상기 화학적 및 구조적 변형은 당해 분야에 공지되어 있으며, 특히 문헌 [Nawrot, Current Topics in Med Chem, 6, 913-925 (2006)]에 예시되어 있다.
dsRNA에 리간드를 접합시키는 것은 그의 세포 흡수 뿐 아니라 특정 조직에 대한 표적화를 증대시킬 수 있다. 특정한 경우에, 소수성 리간드를 dsRNA에 접합시켜 세포막의 직접적 투과를 촉진시킨다. 또는, dsRNA에 접합된 리간드는 수용체-매개 세포이물흡수(endocytosis)를 위한 기질이다. 이러한 접근방법은 안티센스 올리고뉴클레오티드의 세포 투과를 촉진하기 위해 사용되어 왔다. 예를 들면, 콜레스테롤은 다양한 안티센스 올리고뉴클레오티드에 접합되어 그의 비-접합 유사체에 비해 훨씬 더 활성인 화합물을 야기하였다(문헌 [M. Manoharan, Antisense & Nucleic Acid Drug Development, 12, 103 (2002)] 참조). 올리고뉴클레오티드에 접합된 다른 친유성 화합물로는 1-피렌 부티르산, 1,3-비스-O-(헥사데실)글리세롤 및 멘톨이 포함된다. 수용체-매개 세포이물흡수를 위한 리간드의 한 예는 폴산이다. 폴산은 폴레이트-수용체-매개 세포이물흡수에 의해 세포에 유입된다. 폴산을 갖는 dsRNA 화합물은 폴레이트-수용체-매개 세포이물흡수에 의해 효과적으로 세포내로 이동된다. 올리고뉴클레오티드의 3'-말단에 폴산의 부착은 올리고뉴클레오티드의 증가된 세포 흡수를 야기한다[Li, S.; Deshmukh, H.M.; Huang, L. Pharm. Res., 15, 1540 (1998)]. 올리고뉴클레오티드에 접합된 다른 리간드로는 폴리에틸렌 글라이콜, 탄수화물 군, 가교결합제, 포르피린 접합체 및 전달 펩티드가 포함된다.
특정한 경우에, 올리고뉴클레오티드에 대한 양이온성 리간드의 접합은 종종 뉴클레아제에 대한 개선된 저항성을 야기한다. 양이온성 리간드의 대표적인 예는 프로필암모늄 및 다이메틸프로필암모늄이다. 흥미롭게도, 안티센스 올리고뉴클레오티드는 양이온성 리간드가 올리고뉴클레오티드 전체에 분산되었을 때 mRNA에 대한 그의 높은 결합 친화도를 유지하는 것으로 보고되었다(문헌 [M. Manoharan, Antisense & Nucleic Acid Drug Development, 12, 103 (2002)] 및 그 안의 참고문헌 참조).
본 발명의 리간드-접합된 dsRNA는, dsRNA 상에 연결 분자의 부착으로부터 유도된 것과 같은 펜던트 반응성 작용기를 갖는 dsRNA의 사용에 의해 합성될 수 있다. 상기 반응성 올리고뉴클레오티드는 상업적으로 시판되는 리간드, 임의의 다양한 보호기를 갖도록 합성된 리간드, 또는 그에 결합된 연결 잔기를 갖는 리간드와 직접 반응할 수 있다. 본 발명의 방법은, 일부 바람직한 태양에서, 리간드와 적절히 접합되고 고체-지지체 물질에 추가로 부착될 수 있는 뉴클레오시드 단량체의 사용에 의해 리간드-접합된 dsRNA의 합성을 촉진한다. 선택적으로 고체-지지체 물질에 부착된 상기 리간드-뉴클레오티드 접합체는, 선택된 혈청-결합 리간드와 뉴클레오시드 또는 올리고뉴클레오티드의 5' 위치상에 위치한 연결 잔기와의 반응에 의해, 본 발명의 방법의 일부 바람직한 태양에 따라 제조된다. 특정한 경우에, dsRNA의 3'-말단에 부착된 아르알킬 리간드를 갖는 dsRNA는 단량체 구성 요소를 장쇄 아미노알킬기에 의해 기공 조절 유리(controlled-pore-glass) 지지체에 먼저 공유적으로 부착시킴으로써 제조된다. 이어서, 뉴클레오티드는 표준 고체상 합성 기술에 의해 고체 지지체에 결합된 단량체 구성 요소에 결합된다. 단량체 구성 요소는 고체상 합성에 상용성인 뉴클레오시드 또는 기타 유기 화합물일 수 있다.
본 발명의 접합체에 사용된 dsRNA는 공지된 고체상 합성 기술을 통해 편리하게 통상적으로 제조될 수 있다. 또한, 포스포로티오에이트 및 알킬화 유도체와 같은 다른 올리고뉴클레오티드를 제조하기 위해 유사한 기술을 사용하는 것도 알려져 있다.
특정한 변형 올리고뉴클레오티드의 합성에 관한 기술은 하기의 미국 특허에서 찾을 수 있다: 폴리아민 접합된 올리고뉴클레오티드에 관한 미국 특허 제 5,218,105 호; 변형된 주쇄를 갖는 올리고뉴클레오티드에 관한 미국 특허 제 5,541,307 호; 키랄 인 결합을 갖는 올리고뉴클레오티드의 제조 방법에 관한 미국 특허 제 5,521,302 호; 펩티드 핵산에 관한 미국 특허 제 5,539,082 호; β-락탐 주쇄를 갖는 올리고뉴클레오티드에 관한 미국 특허 제 5,554,746 호; 올리고뉴클레오티드의 합성을 위한 방법 및 물질에 관한 미국 특허 제 5,571,902 호; 뉴클레오시드의 임의의 다양한 위치에 부착된 다른 잔기에 링커로서 사용될 수 있는 알킬티오기를 갖는 뉴클레오시드에 관한 미국 특허 제 5,578,718 호; 고 키랄 순도의 포스포로티오에이트 결합을 갖는 올리고뉴클레오티드에 관한 미국 특허 제 5,587,361 호; 2,6-다이아미노퓨린 화합물을 포함하여, 2'-O-알킬 구아노신 및 관련 화합물의 제조 방법에 관한 미국 특허 제 5,506,351 호; N-2 치환된 퓨린을 갖는 올리고뉴클레오티드에 관한 미국 특허 제 5,587,469 호; 3-데아자퓨린을 갖는 올리고뉴클레오티드에 관한 미국 특허 제 5,587,470 호; 접합된 4'-데스메틸 뉴클레오시드 유사체에 관한 미국 특허 제 5,608,046 호; 주쇄-변형된 올리고뉴클레오티드 유사체에 관한 미국 특허 제 5,610,289 호; 특히 2'-플루오로-올리고뉴클레오티드의 합성 방법에 관한 미국 특허 제 6,262,241 호.
리간드-접합된 dsRNA, 및 본 발명의 서열-특이적으로 연결된 뉴클레오시드를 갖는 리간드-분자에서, 올리고뉴클레오티드 및 올리고뉴클레오시드는 표준 뉴클레오티드 또는 뉴클레오시드 전구체, 또는 이미 연결 잔기를 갖는 뉴클레오티드 또는 뉴클레오시드 접합체 전구체, 이미 리간드 분자를 갖는 리간드-뉴클레오티드 또는 뉴클레오시드-접합체 전구체, 또는 비-뉴클레오시드 리간드-함유 구성 요소를 사용하여 적당한 DNA 합성기 상에서 구성될 수 있다.
이미 연결 잔기를 갖는 뉴클레오티드-접합체 전구체를 사용하는 경우, 서열-특이적으로 연결된 뉴클레오시드의 합성은 전형적으로 완성되며, 리간드 분자는 이어서 연결 잔기와 반응하여 리간드-접합된 올리고뉴클레오티드를 형성한다. 스테로이드, 비타민, 지질 및 리포터 분자와 같은 다양한 분자를 갖는 올리고뉴클레오티드 접합체는 이미 기술되었다(마노하란(Manoharan) 등의 PCT 출원 WO 93/07883 호 참조). 바람직한 태양에서, 본 발명의 올리고뉴클레오티드 또는 연결된 뉴클레오시드는 상업적으로 시판하는 포스포르아미다이트 이외에 리간드-뉴클레오시드 접합체로부터 유도된 포스포르아미다이트를 사용하여 자동화된 합성기에 의해 합성된다.
올리고뉴클레오티드의 뉴클레오시드 내에 2'-O-메틸, 2'-O-에틸, 2'-O-프로필, 2'-O-알킬, 2'-O-아미노알킬 또는 2'-데옥시-2'-플루오로기의 혼입은 올리고뉴클레오티드에 증대된 하이브리드화 성질을 제공한다. 또한, 포스포로티오에이트 주쇄를 함유하는 올리고뉴클레오티드는 증대된 뉴클레아제 안정성을 갖는다. 따라서, 본 발명의 작용화된, 연결된 뉴클레오시드는 포스포로티오에이트 주쇄 또는 2'-O-메틸, 2'-O-에틸, 2'-O-프로필, 2'-O-아미노알킬, 2'-O-알킬 또는 2'-데옥시-2'-플루오로기 중 어느 하나 또는 둘 다를 포함하도록 확대시킬 수 있다.
일부 바람직한 태양에서, 5'-말단에 아미노기를 갖는 본 발명의 작용화된 뉴클레오시드 서열은 DNA 합성기를 사용하여 제조하고, 이어서 선택된 리간드의 활성 에스터 유도체와 반응시킨다. 활성 에스터 유도체는 당해 분야에 숙련된 자에게 공지되어 있다. 대표적인 활성 에스터로는 N-하이드로숙신이미드 에스터, 테트라플루오로페놀산 에스터, 펜타플루오로페놀산 에스터 및 펜타클로로페놀산 에스터가 포함된다. 아미노기와 활성 에스터의 반응은 선택된 리간드가 연결기를 통해 5'-위치에 부착된 올리고뉴클레오티드를 생성한다. 5'-말단에서의 아미노기는 5'-아미노-변형제 C6 시약을 사용하여 제조할 수 있다. 바람직한 태양에서, 리간드 분자는, 리간드가 5'-하이드록시기에 직접 연결되거나 또는 링커에 의해 간접적으로 연결된 리간드-뉴클레오시드 포스포르아미다이트의 사용에 의해 5'-위치에서 올리고뉴클레오티드에 접합될 수 있다. 상기 리간드-뉴클레오시드 포스포르아미다이트는 5'-말단에 리간드를 갖는 리간드-접합된 올리고뉴클레오티드를 제공하기 위해 전형적으로 자동화 합성 절차의 마지막에 사용된다.
본 발명 방법의 한 바람직한 태양에서, 리간드 접합된 올리고뉴클레오티드의 제조는 리간드 분자를 구성하기 위한 적절한 전구체 분자의 선택으로 시작된다. 전형적으로, 전구체는 통상적으로 사용되는 뉴클레오시드의 적절히 보호된 유도체이다. 예를 들면, 본 발명의 리간드-접합된 올리고뉴클레오티드의 합성을 위한 합성 전구체로는 분자의 핵염기 부분에서 보호될 수 있는 2'-아미노알콕시-5'-ODMT-뉴클레오시드, 2'-6-아미노알킬아미노-5'-ODMT-뉴클레오시드, 5'-6-아미노알콕시-2'-데옥시-뉴클레오시드, 5'-6-아미노알콕시-2-보호된 뉴클레오시드, 3'-6-아미노알콕시-5'-ODMT-뉴클레오시드 및 3'-아미노알킬아미노-5'-ODMT-뉴클레오시드가 포함되나, 이로 한정되지는 않는다. 상기 아미노-결합된 보호된 뉴클레오시드 전구체의 합성 방법은 당해 분야에 통상의 기술을 가진 자에게 공지되어 있다.
많은 경우에서, 보호기는 본 발명 화합물의 제조동안 사용된다. 본원에서 사용된 바와 같이, 용어 "보호된"은 표시된 잔기가 그 위에 결합된 보호기를 갖는 것을 의미한다. 본 발명의 일부 바람직한 태양에서, 화합물은 하나 이상의 보호기를 함유한다. 매우 다양한 보호기를 본 발명의 방법에 사용할 수 있다. 일반적으로, 보호기는 화학 작용기를 특정 반응 조건에 불활성이 되게 하며, 분자의 나머지 부분을 실질적으로 손상시키지 않고 분자중의 상기 작용기에 결합되고 그로부터 제거될 수 있다.
대표적인 하이드록실 보호기 및 기타 대표적인 보호기는 문헌 [Greene and Wuts, Protective Groups in Organic Synthesis, Chapter 2, 24 ed., John Wiley & Sons, New York (1991); and Oligonucleotides And Analogues A Practical Approach, Ekstein, F. Ed., IRL Press, N.Y. (1991)]에 개시되어 있다.
산 처리에 안정한 아미노-보호기는 염기 처리에 의해 선택적으로 제거되며, 치환에 선택적으로 이용가능한 반응성 아미노기를 제조하기 위해 사용된다. 상기 기의 예는 Fmoc[E. Atherton and R.C. Sheppard in The Peptides, S. Udenfriend, J. Meienhofer, Eds., Academic Press, Orlando, volume 9, p.1 (1987)] 및 Nsc 기[Samukov et al., Tetrahedron Lett., 35:7821 (1994)]로 예시되는 다양한 치환된 설포닐에틸 카바메이트이다.
추가의 아미노-보호기로는 카바메이트 보호기, 예를 들면, 2-트라이메틸실릴에톡시카본일(Teoc), 1-메틸-1-(4-바이페닐일)에톡시카본일(Bpoc), t-부톡시카본일(BOC), 알릴옥시카본일(Alloc), 9-플루오레닐메틸옥시카본일(Fmoc) 및 벤질옥시카본일(Cbz); 아미드 보호기, 예를 들면, 폼일, 아세틸, 트라이할로아세틸, 벤조일 및 니트로페닐아세틸; 설폰아미드 보호기, 예를 들면, 2-니트로벤젠설폰일; 및 이민 및 환형 이미드 보호기, 예를 들면, 프탈이미도 및 다이티아숙시노일이 포함되나, 이로 한정되지는 않는다. 이들 아미노-보호기의 등가물도 또한 본 발명의 화합물 및 방법에 포함된다.
많은 고체 지지체가 상업적으로 시판되며, 당해 분야에 통상의 기술을 가진 자라면 고체상 합성 단계에서 사용되기 위한 고체 지지체를 용이하게 선택할 수 있다. 특정 태양에서, 일반 지지체를 사용한다. 일반 지지체는 올리고뉴클레오티드의 3'-말단에 위치한 특이하거나 변형된 뉴클레오티드를 갖는 올리고뉴클레오티드의 제조를 가능케 한다. 일반 지지체에 대한 추가의 상세한 설명은 문헌 [Scott et al., Innovations and Perspectives in solid-phase Synthesis, 3rd International Symposium, Ed. Roger Epton, Mayflower Worldwide, 115-124 (1994)]을 참조하시오. 또한, 올리고뉴클레오티드는 보다 쉽게 염기성 가수분해가 일어나는 syn-1,2-아세톡시포스페이트기를 통해 고체상에 결합되는 경우 보다 온화한 반응 조건하에서 일반 지지체로부터 분리될 수 있는 것으로 보고되었다(문헌 [Guzaev, A.I.; Manoharan, M.J. Am. Chem. Soc., 125, 2380 (2003)] 참조).
뉴클레오시드는 인-함유 또는 비-인-함유 뉴클레오시드간 공유 결합에 의해 연결된다. 확인을 위해, 상기 접합된 뉴클레오시드는 리간드-함유 뉴클레오시드 또는 리간드-뉴클레오시드 접합체로 규정될 수 있다. 그 서열내에 뉴클레오시드에 접합된 아르알킬 리간드를 갖는 연결된 뉴클레오시드는 접합되지 않은 유사 dsRNA 화합물과 비교할 때 증대된 dsRNA 활성을 나타낼 것이다.
본 발명의 아르알킬-리간드-접합된 올리고뉴클레오티드는 또한 올리고뉴클레오티드 및 연결된 뉴클레오시드의 접합체를 포함하는데, 이때 리간드는 연결기의 중개없이 뉴클레오시드 또는 뉴클레오티드에 직접 부착된다. 리간드는 바람직하게는 리간드의 카복실, 아미노 또는 옥소 기에서 연결기에 의해 부착될 수 있다. 전형적인 연결기는 에스터, 아미드 또는 카바메이트 기일 수 있다.
본 발명의 리간드-접합된 올리고뉴클레오티드에 사용하기 위해 고려되는 바람직한 변형된 올리고뉴클레오티드의 특정 예로는 변형된 주쇄 또는 비-천연 뉴클레오시드간 결합을 함유하는 올리고뉴클레오티드가 포함된다. 본원에서 정의한 바와 같이, 변형된 주쇄 또는 뉴클레오시드간 결합을 갖는 올리고뉴클레오티드로는 주쇄에 인 원자를 보유하는 것들 및 주쇄에 인 원자를 갖지 않는 것들이 포함된다. 본 발명에 있어서, 그의 당간(intersugar) 주쇄에 인 원자를 갖지 않는 변형된 올리고뉴클레오티드는 또한 올리고뉴클레오시드인 것으로 간주될 수 있다.
특정 올리고뉴클레오티드 화학적 변형은 하기에 기술한다. 주어진 화합물 중 모든 위치가 균일하게 변형될 필요는 없다. 역으로, 하나보다 많은 변형이 단일 dsRNA 화합물에 또는 심지어 그의 단일 뉴클레오티드에 혼입될 수 있다.
바람직한 변형된 뉴클레오시드간 결합 또는 주쇄로는, 예를 들면, 포스포로티오에이트, 키랄 포스포로티오에이트, 포스포로다이티오에이트, 포스포로트라이에스터, 아미노알킬포스포로트라이에스터, 메틸 및 다른 알킬 포스포네이트, 예를 들면, 3'-알킬렌 포스포네이트 및 키랄 포스포네이트, 포스피네이트, 포스포르아미데이트, 예를 들면, 3'-아미노 포스포르아미데이트 및 아미노알킬포스포르아미데이트, 티오노포스포르아미데이트, 티오노알킬포스포네이트, 티오노알킬포스포트라이에스터, 및 통상적인 3'-5' 결합을 갖는 보라노포스페이트, 이들의 2'-5' 연결된 유사체, 및 뉴클레오시드 단위의 인접 쌍이 3'-5'에서 5'-3' 또는 2'-5'에서 5'-2'로 연결된 역 극성을 갖는 것들이 포함된다. 다양한 염, 혼합 염 및 유리-산 형태도 또한 포함된다.
상기 인-원자 함유 결합의 제조에 관한 대표적인 미국 특허로는 다음이 포함되나, 이로 한정되지는 않는다: 각각 본원에 참고로 인용된 미국 특허 제 4,469,863; 5,023,243; 5,264,423; 5,321,131; 5,399,676; 5,405,939; 5,453,496; 5,455,233 및 5,466,677 호.
그 중에 인 원자를 포함하지 않는 바람직한 변형된 뉴클레오시드간 결합 또는 주쇄(즉, 올리고뉴클레오시드)는 단쇄 알킬 또는 사이클로알킬 당간 결합, 혼합된 헤테로원자 및 알킬 또는 사이클로알킬 당간 결합, 또는 하나 이상의 단쇄 헤테로원자 또는 헤테로사이클릭 당간 결합에 의해 형성되는 주쇄를 갖는다. 이들로는 모르폴리노 결합(뉴클레오시드의 당 부분으로부터 부분적으로 형성됨)을 갖는 주쇄; 실록산 주쇄; 설파이드, 설폭사이드 및 설폰 주쇄; 폼아세틸 및 티오폼아세틸 주쇄; 메틸렌 폼아세틸 및 티오폼아세틸 주쇄; 알켄 함유 주쇄; 설파메이트 주쇄; 메틸렌이미노 및 메틸렌하이드라지노 주쇄; 설포네이트 및 설폰아미드 주쇄; 아미드 주쇄; 및 N, O, S 및 CH2 성분 부분들을 갖는 기타 주쇄가 포함된다.
상기 올리고뉴클레오시드의 제조에 관한 대표적인 미국 특허로는 각각 본원에 참고로 인용된 미국 특허 제 5,034,506; 5,214,134; 5,216,141; 5,264,562; 5,466,677; 5,470,967; 5,489,677; 5,602,240 및 5,663,312 호가 포함되나, 이들로 한정되지는 않는다.
다른 바람직한 올리고뉴클레오티드 모방체에서는, 당, 및 뉴클레오시드 단위의 뉴클레오시드간 결합, 즉, 주쇄가 둘 다 새로운 기로 치환된다. 핵염기 단위는 적절한 핵산 표적 화합물과의 하이브리드화를 위해 유지된다. 탁월한 하이브리드화 특성을 갖는 것으로 나타난 하나의 상기 올리고뉴클레오티드, 올리고뉴클레오티드 모방체는 펩티드 핵산(PNA)으로 지칭된다. PNA 화합물에서, 올리고뉴클레오티드의 당-주쇄는 아미드-함유 주쇄, 특히 아미노에틸글라이신 주쇄로 치환된다. 핵염기는 유지되며 주쇄의 아미드 부분의 원자들에 직접 또는 간접적으로 결합된다. PNA 화합물에 대한 교지는, 예를 들면, 미국 특허 제 5,539,082 호에서 찾을 수 있다.
본 발명의 일부 바람직한 태양은 포스포로티오에이트 결합을 갖는 올리고뉴클레오티드 및 헤테로원자 주쇄를 갖는 올리고뉴클레오시드, 및 특히, 상기에서 참조한 미국 특허 제 5,489,677 호의 --CH2--NH--O--CH2--, --CH2--N(CH3)--O--CH2--[메틸렌(메틸이미노) 또는 MMI 주쇄로도 알려짐], --CH2--O--N(CH3)--CH2--, --CH2--N(CH3)--N(CH3)--CH2-- 및 --O--N(CH3)--CH2--CH2--[여기서 본래의 포스포다이에스터 주쇄는 --O--P--O--CH2--로 표현된다], 및 상기 참조한 미국 특허 제 5,602,240 호의 아미드 주쇄를 사용한다. 상기 참조한 미국 특허 제 5,034,506 호의 모르폴리노 주쇄 구조를 갖는 올리고뉴클레오티드도 또한 바람직하다.
본 발명의 리간드-접합된 올리고뉴클레오티드에 사용되는 올리고뉴클레오티드는 추가로 또는 대안적으로 핵염기(종종 당해 분야에서는 간단히 "염기"로 지칭함) 변형 또는 치환을 포함할 수 있다. 본원에서 사용되는 바와 같이, "비변형" 또는 "천연" 핵염기로는 퓨린 염기 아데닌(A) 및 구아닌(G), 및 피리미딘 염기 티민(T), 시토신(C) 및 우라실(U)이 포함된다. 변형된 핵염기로는 다른 합성 및 천연 핵염기, 예를 들면, 5-메틸시토신(5-me-C), 5-하이드록시메틸 시토신, 잔틴, 하이포잔틴, 2-아미노아데닌, 아데닌 및 구아닌의 6-메틸 및 다른 알킬 유도체, 아데닌 및 구아닌의 2-프로필 및 다른 알킬 유도체, 2-티오우라실, 2-티오티민 및 2-티오시토신, 5-할로우라실 및 시토신, 5-프로피닐 우라실 및 시토신, 6-아조 우라실, 시토신 및 티민, 5-우라실(슈도우라실), 4-티오우라실, 8-할로, 8-아미노, 8-티올, 8-티오알킬, 8-하이드록실 및 기타 8-치환된 아데닌 및 구아닌, 5-할로, 특히 5-브로모, 5-트라이플루오로메틸 및 기타 5-치환된 우라실 및 시토신, 7-메틸구아닌 및 7-메틸아데닌, 8-아자구아닌 및 8-아자아데닌, 7-데아자구아닌 및 7-데아자아데닌, 및 3-데아자구아닌 및 3-데아자아데닌이 포함된다.
또 다른 핵염기로는 미국 특허 제 3,687,808 호에 개시된 것들, 문헌 [the Concise Encyclopedia Of Polymer Science And Engineering, pages 858-859, Kroschwitz, J.I., ed. John Wiley & Sons, (1990)]에 개시된 것들, 문헌 [Englisch et al., Angewandte Chemie, International Edition, 30, 613 (1991)]에 개시된 것들, 및 문헌 [Sanghvi, Y.S., Chapter 15, Antisense Research and Applications, pages 289-302, Crooke, S.T. and Lebleu, B., ed., CRC Press (1993)]에 개시된 것들이 포함된다. 이들 핵염기중 특정한 것들은 본 발명의 올리고뉴클레오티드의 결합 친화도를 증가시키는데 특히 유용하다. 이들로는 2-아미노프로필아데닌, 5-프로핀일우라실 및 5-프로핀일시토신을 포함하여, 5-치환된 피리미딘, 6-아자피리미딘 및 N-2, N-6 및 O-6 치환된 퓨린이 포함된다. 5-메틸시토신 치환은 핵산 이중 안정성을 0.6 내지 1.2 ℃만큼 증가시키는 것으로 나타났으며(동일 문헌, 276 내지 278 페이지), 본 발명에 바람직한 염기 치환이며, 2'-메톡시에틸 당 변형과 조합되는 경우 훨씬 더 특히 바람직하다.
상기 언급한 변형된 핵염기 중 특정한 염기 및 다른 변형된 핵염기의 제조에 관한 대표적인 미국 특허로는 상기 언급한 미국 특허 제 ,687,808 호, 및 미국 특허 제 5,134,066; 5,459,255; 5,552,540; 5,594,121 및 5,596,091 호가 포함되나, 이들로 한정되지는 않으며, 이들은 모두 본원에 참고로 인용된다.
특정 태양에서, 본 발명의 리간드-접합된 올리고뉴클레오티드에 사용된 올리고뉴클레오티드는 추가로 또는 대안적으로 하나 이상의 치환된 당 잔기를 포함할 수 있다. 바람직한 올리고뉴클레오티드는 2' 위치에 다음 중 하나를 포함한다: OH; F; O-, S- 또는 N-알킬, O-, S- 또는 N-알케닐, 또는 O-, S- 또는 N-알키닐(여기서, 알킬, 알케닐 및 알키닐은 치환되거나 비치환된 C1 내지 C10 알킬 또는 C2 내지 C10 알케닐 및 알키닐일 수 있다). O[(CH2)N0]MCH3, O(CH2)NOCH3, O(CH2)NNH2, O(CH2)NCH3, O(CH2)NONH2 및 O[(CH2)NON[(CH2)NCH3]2(여기서, n 및 m은 1 내지 약 10이다)이 특히 바람직하다. 다른 바람직한 올리고뉴클레오티드는 2' 위치에 다음중 하나를 포함한다: C1 내지 C10 저급 알킬, 치환된 저급 알킬, 알크아릴, 아르알킬, O-알크아릴 또는 O-아르알킬, SH, SCH3, OCN, Cl, Br, CN, CF3, OCF3, SOCH3, SO2 CH3, ONO2, NO2, N3, NH2, 헤테로사이클로알킬, 헤테로사이클로알크아릴, 아미노알킬아미노, 폴리알킬아미노, 치환된 실릴, RNA 분리기, 리포터기, 인터칼레이터(intercalator), 올리고뉴클레오티드의 약물동력학적 성질을 개선하기 위한 기, 또는 올리고뉴클레오티드의 약력학적 성질을 개선하기 위한 기, 및 유사한 성질을 갖는 다른 치환체들. 바람직한 변형은 2'-메톡시에톡시 [2'--O--CH2CH2OCH3, 2'-O-(2-메톡시에틸) 또는 2'-MOE로도 알려져 있음], 즉, 알콕시알콕시기를 포함한다. 다른 바람직한 변형은 본원에 참고로 인용된, 1998년 1월 30일자로 출원된 미국 특허 제 6,127,533 호에 기술된 바와 같이, 2'-DMAOE로도 알려진 2'-다이메틸아미노옥시에톡시, 즉, O(CH2)2ON(CH3)2 기를 포함한다.
다른 바람직한 변형은 2'-메톡시 (2'-O--CH3), 2'-아미노프로폭시 (2'-OCH2CH2CH2NH2) 및 2'-플루오로 (2'-F)를 포함한다. 유사한 변형도 또한 올리고뉴클레오티드상의 다른 위치에서, 특히 3' 말단 뉴클레오티드 또는 2'-5' 연결된 올리고뉴클레오티드 상의 당의 3' 위치에서 이루어질 수 있다.
본원에서 사용된 바와 같이, 용어 "당 치환기" 또는 "2-치환기"는 산소 원자의 존재 또는 부재하에서 리보푸라노실 잔기의 2'-위치에 부착된 기를 포함한다. 당 치환기로는 플루오로, O-알킬, O-알킬아미노, O-알킬알콕시, 보호된 O-알킬아미노, O-알킬아미노알킬, O-알킬 이미다졸 및 식 (O-알킬)m(여기서 m은 1 내지 약 10이다)의 폴리에터가 포함되나, 이로 한정되지는 않는다. 이들 폴리에터 중에서 선형 및 환형 폴리에틸렌 글라이콜(PEG) 및 (PEG)-함유기, 예를 들면, 크라운 에터, 및 특히 본원에 전체로 참고로 인용된 문헌 [Delgardo et. al., Critical Reviews in Therapeutic Drug Carrier Systems 9:249 (1992)]에 개시된 것들이 바람직하다. 다른 당 변형은 문헌 [Cook, Anti-fibrosis Drug Design, 6:585-607 (1991)]에 개시되어 있다. 플루오로, O-알킬, O-알킬아미노, O-알킬 이미다졸, O-알킬아미노알킬 및 알킬 아미노 치환은 본원에 전체로 참고로 인용된, "2' 및 5' 치환을 갖는 피리미딘 뉴클레오티드(들)을 갖는 올리고머 화합물(Oligomeric Compounds having Pyrimidine Nucleotide(s) with 2' and 5' Substitutions)"이란 발명의 명칭을 갖는 미국 특허 제 6,166,197 호에 기술되어 있다.
본 발명에 따르는 또 다른 당 치환기로는 2'-SR 및 2'-NR2 기가 포함되며, 여기서 R은 각각 독립적으로 수소, 보호기, 또는 치환되거나 비치환된 알킬, 알케닐 또는 알키닐이다. 2'-SR 뉴클레오시드는 본원에 전체로 참고로 인용된 미국 특허 제 5,670,633 호에 개시되어 있다. 2'-SR 단량체 합성단위체(synthon)의 혼입은 문헌 [Hamm et al., J. Org. Chem., 62:3415-3420 (1997)]에 개시되어 있다. 2'-NR 뉴클레오시드는 문헌 [Goettingen, M., J. Org. Chem., 61, 6273-6281 (1996); and Polushin et al., Tetrahedron Lett., 37, 3227-3230 (1996)]에 개시되어 있다. 본 발명에 따른 또 다른 대표적인 2'-치환기로는 하기 화학식 I 또는 II 중 하나를 갖는 것들이 포함된다:
[화학식 I]
[화학식 II]
상기 식에서,
E는 C1-C10 알킬, N(Q3)(Q4) 또는 N=C(Q3)(Q4)이고; Q3 및 Q4는 각각 독립적으로, H, C1-C10 알킬, 다이알킬아미노알킬, 질소 보호기, 고정되거나 비고정된 공액 기, 고치 지지체에 대한 링커이거나; 또는 Q3 및 Q4는 함께 질소 보호기, 또는 N 및 O로부터 선택된 하나 이상의 추가의 헤테로원자를 포함하거나 포함하지 않는 고리 구조를 형성하며;
q1은 1 내지 10의 정수이고;
q2는 1 내지 10의 정수이고;
q3는 0 또는 1이고;
q4는 0, 1 또는 2이고;
Z1, Z2 및 Z3는 각각 독립적으로, C4-C7 사이클로알킬, C5-C14 아릴 또는 C3-C15 헤테로사이클릴이며, 상기 헤테로사이클릴기에서 헤테로원자는 산소, 질소 및 황으로부터 선택되고;
Z4는 OM1, SM1 또는 N(M1)2이고; M1은 각각 독립적으로, H, C1-C8 알킬, C1-C8 할로알킬, C(=NH)N(H)M2, C(=O)N(H)M2 또는 OC(=O)N(H)M2이고; M2는 H 또는 C1-C8 알킬이고;
Z5는 C1-C10 알킬, C1-C10 할로알킬, C2-10C 알케닐, C2-C10 알키닐, C6-C14 아릴, N(Q3)(Q4), OQ3, 할로, SQ3 또는 CN이다.
화학식 I의 대표적인 2'-O-당 치환기는 본원에 전체로 참고로 인용된, "캐핑된 2'-옥시에톡시 올리고클레오티드(Capped 2'-Oxyethoxy Oligonucleotides)"라는 발명의 명칭을 갖는 미국 특허 제 6,172, 209 호에 개시되어 있다. 화학식 II의 대표적인 환형 2'-O-당 치환기는 본원에 전체로 참고로 인용된, "형태적으로 미리구성된 RNA 표적화된 2'-변형 올리고뉴클레오티드(RNA Targeted 2'-Modified Oligonucleotides that are Conformationally Preorganized)"라는 발명의 명칭을 갖는 미국 특허 제 6,271,358 호에 개시되어 있다.
리보실 고리상에 O-치환을 갖는 당도 또한 본 발명에 따른다. 고리 O에대한 대표적인 치환은 S, CH2, CHF 및 CF2를 포함하나, 이로 한정되지는 않는다.
올리고뉴클레오티드는 또한 펜토푸라노실 당 대신 당 모방체, 예를 들면, 사이클로부틸 잔기를 가질 수 있다. 상기 변형된 당의 제조에 관한 대표적인 미국 특허로는 모두 본원에 참고로 인용된 미국 특허 제 5,359,044; 5,466,786; 5,519,134; 5,591,722; 5,597,909; 5,646,265 및 5,700,920 호가 포함되나, 이들로 한정되지는 않는다.
추가의 변형이 올리고뉴클레오티드의 다른 위치, 특히, 3' 말단 뉴클레오티드 상의 당의 3' 위치에서 이루어질 수 있다. 예를 들면, 본 발명의 리간드-접합된 올리고뉴클레오티드의 하나의 추가 변형은, 올리고뉴클레오티드의 활성, 세포 분포 또는 세포 흡수를 증대시키는 하나 이상의 추가의 비-리간드 잔기 또는 접합체를 올리고뉴클레오티드에 화학적으로 연결시키는 것을 포함한다. 상기 잔기로는 지질 잔기, 예를 들면, 콜레스테롤 잔기[Letsinger et al., Proc. Natl. Acad. Sci. USA, 86, 6553 (1989)], 콜산[Manoharan et al., Bioorg. Med. Chem. Lett., 4, 1053 (1994)], 티오에터, 예를 들면, 헥실-S-트라이틸티올[Manoharan et al., Ann. N.Y. Acad. Sci., 660, 306 (1992); Manoharan et al., Bioorg. Med. Chem. Let., 3, 2765 (1993)], 티오콜레스테롤[Oberhauser et al., Nucl. Acids Res., 20, 533 (1992)], 지방족쇄, 예를 들면, 도데칸다이올 또는 운데실 잔기[Saison-Behmoaras et al., EMBO J., 10, 111 (1991); Kabanov et al., FEBS Lett., 259, 327 (1990); Svinarchuk et al., Biochimie, 75, 49 (1993)], 인지질, 예를 들면, 다이-헥사데실-rac-글리세롤 또는 트라이에틸암모늄 1,2-다이-O-헥사데실-rac-글리세로-3-H-포스포네이트[Manoharan et al., Tetrahedron Lett., 36, 3651 (1995); Shea et al., Nucl. Acids Res., 18, 3777 (1990)], 폴리아민 또는 폴리에틸렌 글라이콜 쇄[Manoharan et al., Nucleosides & Nucleotides, 14, 969 (1995)] 또는 아다만탄 아세트산[Manoharan et al., Tetrahedron Lett., 36, 3651 (1995)], 팔미틸 잔기[Mishra et al., Biochim. Biophys. Acta, 1264, 229 (1995)], 또는 옥타데실아민 또는 헥실아미노-카본일-옥시콜레스테롤 잔기[Crooke et al., J. Pharmacol. Exp. Ther., 277, 923 (1996)]이 포함되나, 이들로 한정되지는 않는다.
본 발명은 또한 올리고뉴클레오티드 내의 특정 위치에 대해 실질적으로 키랄적으로 순수한 올리고뉴클레오티드를 사용하는 조성물을 포함한다. 실질적으로 키랄적으로 순수한 올리고뉴클레오티드의 예로는 75% 이상 Sp 또는 Rp인 포스포로티오에이트 결합을 갖는 것들(쿡(Cook) 등, 미국 특허 제 5,587,361 호) 및 실질적으로 키랄적으로 순수한(Sp 또는 Rp) 알킬포스포네이트, 포스포르아미데이트 또는 포스포트라이에스터 결합을 갖는 것들(쿡, 미국 특허 제 5,212,295 및 5,521,302 호)이 포함되나, 이로 한정되지는 않는다.
특정한 경우에, 올리고뉴클레오티드는 비-리간드 기에 의해 변형될 수 있다. 많은 비-리간드 분자가 올리고뉴클레오티드의 활성, 세포 분포 또는 세포 흡수를 증대시키기 위해 올리고뉴클레오티드에 접합되었으며, 상기 접합을 수행하기 위한 절차는 과학 문헌에서 찾을 수 있다. 상기 비-리간드 잔기로는 지질 잔기, 예를 들면, 콜레스테롤[Letsinger et al., Proc. Natl. Acad. Sci. USA, 86:6553 (1989)], 콜산[Manoharan et al., Bioorg. Med. Chem. Lett., 4:1053 (1994)], 티오에터, 예를 들면, 헥실-S-트라이틸티올[Manoharan et al., Ann. N.Y. Acad. Sci., 660:306 (1992); Manoharan et al., Bioorg. Med. Chem. Let., 3:2765 (1993)], 티오콜레스테롤[Oberhauser et al., Nucl. Acids Res., 20:533 (1992)], 지방족쇄, 예를 들면, 도데칸다이올 또는 운데실 잔기[Saison-Behmoaras et al., EMBO J., 10:111 (1991); Kabanov et al., FEBS Lett., 259:327 (1990); Svinarchuk et al., Biochimie, 75:49 (1993)], 인지질, 예를 들면, 다이-헥사데실-rac-글리세롤 또는 트라이에틸암모늄 1,2-다이-O-헥사데실-rac-글리세로-3-H-포스포네이트[Manoharan et al., Tetrahedron Lett., 36:3651 (1995); Shea et al., Nucl. Acids Res., 18:3777 (1990)], 폴리아민 또는 폴리에틸렌 글라이콜 쇄[Manoharan et al., Nucleosides & Nucleotides, 14:969 (1995)] 또는 아다만탄 아세트산[Manoharan et al., Tetrahedron Lett., 36:3651 (1995)], 팔미틸 잔기[Mishra et al., Biochim. Biophys. Acta, 1264:229 (1995)], 또는 옥타데실아민 또는 헥실아미노-카본일-옥시콜레스테롤 잔기[Crooke et al., J. Pharmacol. Exp. Ther., 277:923 (1996)]이 포함되었다. 전형적인 접합 프로토콜은 서열의 하나 이상의 위치에 아미노링커를 갖는 올리고뉴클레오티드의 합성을 포함한다. 이어서, 아미노기를 적절한 커플링제 또는 활성화제를 사용하여 접합되는 분자와 반응시킨다. 접합 반응은 고체 지지체에 여전히 결합되어 있는 올리고뉴클레오티드를 사용하여 수행하거나, 또는 액상에서 올리고뉴클레오티드를 분리한 후에 수행할 수 있다. HPLC에 의한 올리고뉴클레오티드 접합체의 정제는 전형적으로 순수한 접합체를 제공한다. 콜레스테롤 접합체의 사용은, 상기 잔기가 PTP1B 단백질 생성 부위인 간에서 조직에 대한 표적화를 증가시킬 수 있기 때문에 특히 바람직하다.
또는, 접합되는 분자는 분자에 존재하는 알콜기에 의해 또는 포스포릴화될 수 있는 알콜기를 갖는 링커의 부착에 의해, 포스포르아미다이트와 같은 구성 요소로 전환될 수 있다.
중요하게, 상기 접근방법들은 각각 리간드 접합된 올리고뉴클레오티드의 합성에 사용될 수 있다. 아미노 연결된 올리고뉴클레오티드는 커플링 시약의 사용에 의해 또는 NHS 또는 펜트플루오로페놀레이트 에스터로서 리간드의 활성화 후에 리간드와 직접 커플링될 수 있다. 리간드 포스포르아미다이트는 카복실기 중 하나에 아미노헥산올 링커의 부착 후 말단 알콜 작용기의 포스피틸화에 의해 합성될 수 있다. 시스테아민과 같은 다른 링커도 또한 합성된 올리고뉴클레오티드 상에 존재하는 클로로아세틸 링커에 접합하는데 사용될 수 있다.
달리 정의되지 않는 한, 본원에 사용된 모든 기술적 및 과학적 용어는 본 발명이 속하는 당해 분야에 통상의 기술을 가진 자에 의해 통상적으로 이해되는 바와 동일한 의미를 갖는다. 본원에 기술된 바와 유사하거나 동등한 방법 및 재료를 본 발명의 실시 또는 검사에 사용할 수 있지만, 적당한 방법 및 재료를 하기에서 기술한다. 본원에 언급된 모든 공개공보, 특허출원, 특허 및 기타 참조문헌은 전체로 참고로 인용된다. 상충되는 경우, 정의를 포함하여 본 명세서가 조정할 것이다. 또한, 재료, 방법 및 실시예는 단시 예시적인 것이며 제한하려는 것이 아니다.
본 발명의 상기에 제공된 태양 및 항목들을 이제 하기의 비제한 실시예로 예시한다.
실시예
치료 용도의
dsRNA
의 동정
치료 용도로 인간 PTP1B를 특이적으로 표적화하는 dsRNA를 동정하기 위해 dsRNA 설계를 수행하였다. 먼저, 인간(호모사피엔스) PTP1B의 공지된 mRNA 서열(서열번호 620으로 열거된 NM_002827.2)을 NCBI 유전자은행으로부터 다운로드받았다.
레서스(rhesus) 원숭이(마카카 뮬라타(Macaca mulatta)) PTP1B의 mRNA(XM_001096053.1, XM_001096168.1, XM_001096290.1 및 XM_001096412.1)를 추가로 사용하여 전형적인 공통 mRNA 서열(서열번호 621)을 생성하였다.
상기 서열을 인간 PTP1B mRNA 서열(서열번호 620)과 함께 컴퓨터 분석으로 검사하여 두 서열 모두에 교차반응하는 RNA 간섭(RNAi) 물질을 제공하는 19개 뉴클레오티드의 상동 서열을 동정하였다.
RNAi 물질을 동정하는데 있어서, 선택은, fastA 알고리즘을 사용하여, 포괄적 인간 전사체를 나타내는 것으로 추정한, 인간 RefSeq 데이터베이스(공개 25)중 임의의 다른 서열에 대해 2개 이상의 미스매치를 갖는 19량체 서열로 제한된다.
사이노몰구스 원숭이(마카카 파시큘라리스) PTP1B 유전자를 서열화하고(서열번호 622 참조), RNAi 물질의 표적 영역에 대해 검사하였다.
인간 및 사이노몰구스 원숭이 PTP1B에 교차반응하는 dsRNA가 치료 용도에 가장 바람직한 것으로 규정되었다. 4개 이상의 연속 G를 함유하는 모든 서열(폴리-G 서열)은 합성에서 제외시켰다.
상기와 같이 동정된 서열은 첨부된 표 1에 나타낸 RNAi 물질의 합성에 기초를 이루었다.
생체내
개념 증명 연구를 위한
dsRNA
의 동정
생체내 개념 증명 실험을 위해 마우스(무스 무스큘러스) 및 래트(래터스 노르베기커스)를 표적화하는 dsRNA를 동정하기 위해 dsRNA 설계를 수행하였다. 먼저, 마우스 PTP1B(NM_011201.3, 서열번호 623) 및 래트 PTP1B(NM_012637.2, 서열번호 624)에 대한 전사체를 컴퓨터 분석으로 검사하여 이들 서열사이에 교차반응하는 RNAi 물질을 제공하는 19개 뉴클레오티드의 상동 서열을 동정하였다.
RNAi 물질을 동정하는데 있어, 선택은, fastA 알고리즘을 사용하여, 포괄적 마우스 및 래트 전사체를 나타낸 것으로 추정한, 마우스 및 래트 RefSeq 데이터베이스(공개 25)중 임의의 다른 서열에 대해 안티센스 가닥에 2개 이상의 미스매치를 갖는 19량체 서열로 제한된다.
4개 이상의 연속 G를 함유하는 모든 서열(폴리-G 서열)은 합성에서 제외시켰다. 상기와 같이 동정된 서열은 첨부된 표 4에 나타낸 RNAi 물질의 합성에 기초를 이루었다.
dsRNA
합성
본원에서 시약 공급원을 구체적으로 나타내지 않은 경우, 상기 시약은 분자 생물학에서 적용하기 위한 품질/순도 표준에서 분자 생물학용 시약의 임의의 공급처로부터 수득될 수 있다.
엑스페디트(Expedite) 8909 합성기(어플라이드 바이오시스템즈(Applied Biosystems), 아플레라 도이칠란드 게엠베하(Applera Deutschland GmbH), 독일 다름스타트) 및 고체 지지체로서 기공 조절 유리(CPG, 500 Å, 프롤리고 바이오케미 게엠베하(Proligo Biochemie GemH), 독일 함부르크)를 사용하여 1 μ몰의 규모로 고체상 합성에 의해 단일-가닥 RNA를 생성하였다. RNA, 및 2'-O-메틸 뉴클레오티드를 함유하는 RNA를 상응하는 포스포르아미다이트 및 2'-O-메틸 포스포르아미다이트(프롤리고 바이오케미 게엠베하, 독일 함부르크)를 각각 사용하여 고체상 합성에 의해 생성하였다. 이들 구성 요소는, 문헌 [Current Protocols in nucleic acid chemistry, Beaucage, S.L. et al.(Edrs.), John Wiley & Sons, Inc., New York, NY, USA]에 기술된 바와 같은 표준 뉴클레오시드 포스포르아미다이트 화학을 이용하여 올리고뉴클레오티드 쇄의 서열 내의 선택된 부위에 혼입되었다. 포스포로티오에이트 결합은 요오드 산화제 용액을 아세토니트릴(1%) 중 뷰케이지(Beaucage) 시약(크루아켐 리미티드(Chruachem Ltd), 영국 글래스고우)의 용액으로 대체함으로써 도입하였다. 추가의 보조 시약들은 말린크로트 베이커(Mallinckrodt Baker)(독일, 그리샤임)에서 수득하였다.
음이온 교환 HPLC에 의해 조 올리고리보뉴클레오티드의 탈보호 및 정제를 규정된 절차에 따라 수행하였다. 수율 및 농도는 분광광도계(DU 640B, 벡크만 코울터 게엠베하(Beckman Coulter GmbH), 독일 운터슐레스하임)를 사용하여 260 nm의 파장에서 각각의 RNA의 용액의 UV 흡수에 의해 측정하였다. 어닐링 완충액(20mM 인산나트륨, pH 6.8; 100mM 염화나트륨) 중 상보성 가닥의 동몰 용액을 혼합하여 이중 가닥 RNA를 생성하고, 수조에서 85 내지 90 ℃에서 3 분간 가열하고, 3 내지 4 시간에 걸쳐 실온으로 냉각시켰다. 어닐링된 RNA 용액은 사용할 때까지 -20 ℃에서 보관하였다.
활성 시험
전술한 치료 용도를 위한 PTP1B-dsRNA의 활성을 HpeG2 및 HeLa 세포에서 시험하였다. 배양물 중 세포를, PTP1B-특이적 dsRNA와 함께 배양한 세포로부터 유도된 전체 mRNA 중 분지된 DNA에 의해 PTP1B mRNA의 정량에 사용하였다.
HepG2 세포는 미국 종균 협회(American Type Culture Collection, 미들랜드 로크빌, 카탈로그 번호 HB-8065)에서 입수하였으며, 가습 배양기(헤라유스 헤라셀(Heraeus HERAcell), 켄드로 래버러토리 프로덕츠(Kendro Laboratory Products), 독일 란겐셀볼드)에서 5% CO2의 대기하에 37 ℃에서, 10% 소 태아 혈청(FCS)(바이오크롬 아게(Biochrom AG), 독일 베를린, 카탈로그 번호 S0115), 2mM L-글루타민(바이오크롬 아게, 독일 베를린, 카탈로그 번호 K0238), 페니실린 100 U/ml, 스트렙토마이신 100 mg/ml(바이오크롬 아게, 독일 베를린, 카탈로그 번호 A2213), 1x 비-필수 아미노산(NEA)(바이오크롬 아게, 독일 베를린, 카탈로그 번호 K0293) 및 1mM 피루부산 나트륨(바이오크롬 아게, 독일 베를린, 카탈로그 번호 L0473)을 함유하도록 보충된 MEM(깁코 인비트로겐(Gibco Invitrogen), 인비트로겐 게엠베하(Invitrogen GebH), 독일 칼스루, 카탈로그 번호 21090-022) 중에서 배양하였다.
HeLaS3 세포는 미국 종균 협회(미들랜드 로크빌, 카탈로그 번호 CCL-2.2)에서 입수하였으며, 가습 배양기(헤라유스 헤라셀, 켄드로 래버러토리 프로덕츠, 독일 란겐셀볼드)에서 5% CO2의 대기하에 37 ℃에서, 10% 소 태아 혈청(FCS)(바이오크롬 아게, 독일 베를린, 카탈로그 번호 S0115), 페니실린 100 U/ml, 스트렙토마이신 100 mg/ml(바이오크롬 아게, 독일 베를린, 카탈로그 번호 A2213)을 함유하도록 보충된 Ham's F12(바이오크롬 아게, 독일 베를린, 카탈로그 번호 FG 0815) 중에서 배양하였다.
dsRNA의 세포 접종 및 형질감염은 동시에 수행하였다. dsRNA로 형질감염시키기 위해, HepG2 세포를 2.0 x 104 세포/웰의 밀도로 96-웰 플레이트에 접종하고, HeLaS3 세포는 1.5 x 104 세포/웰의 밀도로 접종하였다. dsRNA의 형질감염은 제작자의 설명에 따라 리포펙트아민 2000(인비트로겐 게엠베하, 독일 칼스루, 카탈로그 번호 11668-019)을 사용하여 수행하였다. 첫 번째 단일 용량 실험에서는, dsRNA를 HepG2 세포중에 30nM의 농도로 형질감염시켰다. 각각의 데이터포인트는 4중으로 측정하였다. 2회의 독립적 실험을 수행하였다. 두 번째 단일 용량 실험에서는, 50% 이상의 PTP1B mRNA 녹다운을 나타내는 대부분의 활성 dsRNA를 HeLaS3 세포에서 30nM로 재분석하였다. HepG2 세포에서 30nM로 첫 번째 단일 용량 선별로부터 60%보다 많은 mRNA 녹다운을 나타내는 가장 효과적인 dsRNA는 용량 반응 곡선에 의해 추가로 특성화하였다. 용량 반응 곡선을 위해, 24, 6, 1.5, 0.375, 0.0938, 0.0234, 0.0059, 0.0015, 0.0004 및 0.0001 nM의 dsRNA 농도(nM)를 사용하는 것을 제외하고, 상기 단일 용량 선별에 대해 기술한 바와 같이 HeLaS3 세포에서 형질감염을 수행하였다. 형질감염 후에 세포를 37 ℃ 및 5% CO2하에 가습 배양기(헤라유스 게엠베하, 독일 하나우)에서 24 시간동안 배양하였다. PTP1B mRNA의 측정을 위해, 세포를 수거하고, mRNA의 bDNA 정량에 대해 퀀티진 익스플로어 키트(Quantigene Explore Kit)(제노스펙트라(Genospectra), 미국 캘리포니아 프리몬트, 카탈로그 번호 QG-000-02)의 제작자가 권장한 절차에 따라 53 ℃에서 세포용해시켰다. 그런 다음, 50 ㎕의 세포용해물을 인간 PTP1B 및 인간 GAPDH에 특이적인 프로브세트(프로브세트의 배열, 첨부된 표 9 및 10 참조)와 함께 배양하고, 퀀티진에 대한 제작자의 프로토콜에 따라 처리하였다. 화학발광을 빅터-2-라이트(Victor-2-light)(퍼킨 엘머(Perkin Elmer), 독일 바이스바덴)에서 RLU(상대 빛단위)로서 측정하고, 인간 PTP1B 프로브세트를 사용하여 수득된 값을 각 웰에 대해 각각의 인간 GAPDH 값으로 표준화하였다. 무관한 대조군 dsRNA를 음성 대조군으로 사용하였다. 마우스 및 래트 PTP1B를 표적화하는 dsRNA의 활성은 dsRNA에 대해 20nM의 농도를 사용하여 MH7777A 세포에서 측정하였으며, 래트 GAPDH 및 마우스/래트 PTP1B의 측정에 특이적인 bDNA 프로브세트는 첨부된 표 11 및 12에 나타내었다.
억제 데이터는 첨부된 표 2 및 4에 나타내었다.
dsRNA
의 안정성
인간 PTP1B를 표적화하는 dsRNA의 경우 인간 혈청 또는 사이노몰구스 원숭이로부터 얻은 혈장을 사용하고, 마우스/래트 PTP1B를 표적화하는 dsRNA의 경우 마우스 혈청을 사용하여 생체내 분석으로 각 단일 가닥의 반감기를 측정함으로써 dsRNA의 안정성을 측정하였다.
30 ㎕의 인간 혈청 또는 사이노몰구스 혈장(시그마 알드리치(Sigma Aldrich))과 혼합된 3 ㎕의 50μM dsRNA를 사용하여, 각 시점에서 3 중으로 측정을 수행하였다. 혼합물을 37 ℃에서 0 분, 30 분, 1 시간, 3 시간, 6 시간, 24 시간 또는 48 시간동안 배양하였다. 비특이적 분해에 대한 대조군으로, dsRNA를 48 ℃에서 30 ㎕의 1x PBS(pH 6.8)와 함께 배양하였다. 65 ℃에서 30 분간 4 ㎕ 프로테이나제 K(20 mg/ml), 25 ㎕의 "조직 및 세포 용해액"(에피센터(Epicentre)) 및 38 ㎕의 밀로포어 워터(Milopore water)를 첨가하여 반응을 중지시켰다. 그런 다음, 샘플을 0.2 ㎛ 96 웰 필터 플레이트를 통해 1400 rpm에서 8 분간 스핀 여과시키고, 55 ㎕의 밀리포어 워터로 2회 세척하고 다시 스핀 여과시켰다.
단일 가닥의 분리 및 잔류하는 전체 길이 생성물(FLP)의 분석을 위해, 용출제 A로서 10% ACN 중의 20mM Na3PO4(pH 11) 및 용출제 B로서 용출제 A 중의 1M NaBr을 사용하여 변성 조건하에서 이온 교환 디오넥스 서밋(Dionex Summit) HPLC에 샘플을 통과시켰다.
다음의 구배를 적용하였다:
매 주입시에, 크로마토그램을 디오넥스 크로멜레온(Dionex Chromeleon) 6.60 HPLC 소프트웨어에 의해 자동으로 통합하고, 경우에 따라 수동으로 조정하였다. 모든 피크 면적은 내부 표준(IS) 피크로 보정하고 t=0 분에서의 배양으로 표준화하였다. 피크 아래 면적 및 생성된 잔류 FLP는 각각의 단일 가닥에 대해 3중으로 따로따로 계산하였다. 가닥의 반감기(t1/2)는 FLP의 절반이 분해되는 평균 시점(h)으로 3 중으로 정의하였다. 결과는 첨부된 표 3 및 5에 나타내었다.
사이토카인 유도
dsRNA의 잠재적 사이토카인 유도는 시험관내 PBMC 분석에서 INF-α 및 TNF-α의 방출을 측정함으로써 검사하였다.
인간 말초혈 단핵 세포(PBMC)는 형질감염 당일에 피콜(Ficoll) 원심분리에 의해 두 공여자의 백혈구 연층(buffy coat) 혈액으로부터 분리하였다. 세포를 dsRNA로 이중으로 형질감염시키고, 진 포터 2(Gene Porter 2)(GP2) 또는 DOTAP를 사용하여, 37 ℃에서 옵티-멤(Opti-MEM) 중 130nM의 최종 농도로 24 시간동안 배양하였다. 상기 분석에서 INF-α 및 TNF-α 뿐 아니라 CpG 올리고를 유도하는 것으로 알려진 dsRNA 서열을 양성 대조군으로 사용하였다. 사이토카인 유도에 형질감염 시약을 필요로 하지 않았던 화학 접합된 dsRNA 또는 CpG 올리고뉴클레오티드를 배양 배지 중에 500nM의 농도로 배양하였다. 배양 마지막에, 배양 상등액을 4 중으로 모았다.
이어서, 풀당 2개의 데이터포인트를 사용하여 표준 샌드위치 ELISA에 의해 상기 모아진 상등액 중에서 IFN-α 및 TNF-α를 측정하였다. 사이토카인 유도 정도는 0 내지 5의 스코어(5가 최대 유도를 나타낸다)를 사용하여 양성 대조군에 대비해 나타내었다. 결과는 첨부된 표 3 및 5에 나타내었다.
인간 PTP1B를 표적화하는 dsRNA의 시험관내 비표적 분석
프사이체크(psiCHECK, 등록상표) - 벡터(프로메가(Promega))는 RNAi 활성을 모니터하기 위한 2개의 리포터 유전자를 함유한다: 레닐라 루시퍼라제(hRluc) 유전자의 합성 형태 및 합성 반딧불이 루시퍼라제 유전자(hluc+). 반딧불이 루시퍼라제 유전자는 레닐라 루시퍼라제 발현에서의 변화를 반딧불이 루시퍼라제 발현으로 표준화시킨다. 레닐라 및 반딧불이 루시퍼라제 활성은 듀얼-글로(Dual-Glo, 등록상표) 루시퍼라제 분석 시스템(프로메가)을 이용하여 측정하였다. 본 발명의 dsRNA의 비표적 효과를 분석하기 위해 프사이체크(등록상표) 벡터를 사용하기 위해, 예측된 비표적 서열을 합성 레닐라 루시퍼라제 유전자 및 그의 번역 정지 코돈에 대해 3'에 위치한 다중 클로닝 영역 내에 클로닝하였다. 클로닝후에, 벡터를 포유동물 세포주에 형질감염시키고, 이어서 PTP1B를 표적화하는 dsRNA로 동시형질감염시켰다. dsRNA가 예측된 비표적의 표적 RNA에 대해 RNAi 과정을 효과적으로 개시하는 경우, 융합된 레닐라 표적 유전자 mRNA 서열이 분해되어 감소된 레닐라 루시퍼라제 활성이 얻어질 것이다.
인
실리코
비표적
예측
본 발명의 dsRNA에 상동성인 서열에 대해 컴퓨터 분석으로 인간 게놈을 검색하였다. 본 발명의 dsRNA와 5개 미만의 미스매치를 나타낸 상동 서열을 가능한 비표적 서열로 규정하였다. 시험관내 비표적 분석을 위해 선택된 비표적 서열을 첨부된 표 6, 7 및 8에 나타내었다.
예측된
비표적
서열을 함유하는
프사이체크
벡터의 생성
dsRNA 리드 후보물질에 대한 비표적 효과를 분석하기 위한 방법은 XhoI 및 NotI 제한효소 부위를 통해 프사이체크2 벡터 시스템(듀얼 글로(등록상표) 시스템, 프로메가, 독일 브라운슈바이크, 카탈로그 번호 C8021) 내에 예측된 비표적 부위를 클로닝하는 것을 포함한다. 그러므로, 비표적 부위는 dsRNA 표적 부위의 상위 및 하위 10개 뉴클레오티드와 함께 연장된다. 또한, NheI 제한효소 부위를 통합시켜 제한효소 분석에 의한 단편의 삽입을 입증한다. 단일-가닥 올리고뉴클레오티드는 마스터사이클러(Mastercycler)(에펜도르프(Eppendorf))에서 표준 프로토콜(예를 들면, 메타비온(Metabion)에 의한 프로토콜)에 따라 어닐링한 후, XhoI 및 NotI으로 미리 절단된 프사이체크(프로메가) 내에 클로닝하였다. 성공적인 삽입은 NheI을 사용한 제한효소 분석 및 이어진 양성 클론의 서열분석에 의해 확인되었다. 서열분석을 위해 선택된 프라이머(서열번호 625)는 벡터 프사이체크의 1401 위치에서 결합한다. 클론 생성후에, 플라스미드를 서열분석에 의해 분석한 후 세포 배양 실험에 사용하였다.
dsRAN
비표적
효과의 분석
세포 배양:
Cos7 세포를 독일 미생물 종균 협회(Deutsche Sammlung fur Mikroorganismen und Zelkulturen, DSMZ, 독일 브라운슈바이크, 카탈로그 번호 ACC-60)로부터 수득하였으며, 가습 배양기(헤라유스 헤라셀, 켄드로 래버러토리 프로덕츠, 독일 란겐셀볼드)에서 5% CO2하의 대기중에서 37 ℃에서, 10% 소 태아 혈청(FCS)(바이오크롬 아게, 독일 베를린, 카탈로그 번호 S0115), 페니실린 100 U/ml 및 스트렙토마이신 100 ㎍/ml(바이오크롬 아게, 독일 베를린, 카탈로그 번호 A2213) 및 2mM L-글루타민(바이오크롬 아게, 독일 베를린, 카탈로그 번호 K0238), 및 12 ㎍/ml 중탄산나트륨을 함유하도록 보충된 DMEM(바이오크롬 아게, 독일 베를린, 카탈로그 번호 F0435) 중에서 배양하였다.
형질감염 및 루시퍼라제 정량:
플라스미드로 형질감염시키기 위해, Cos7 세포를 96-웰 플레이트에 2.25 x 104 세포/웰의 밀도로 접종하고 직접 형질감염시켰다. 플라스미드의 형질감염은 50 ng/웰의 농도에서 제작자가 설명한 대로 리포펙트아민 2000(인비트로겐 게엠베하, 독일 칼스루, 카탈로그 번호 11668-019)을 사용하여 수행하였다. 형질감염 4 시간후에, 배지를 폐기하고 새로운 배지를 첨가하였다. 이어서, 전술한 바와 같이 리포펙트아민 2000을 사용하여 50nM의 농도에서 dsRNA를 형질감염시켰다. dsRNA 형질감염 24 시간 후에, 제작자가 설명한 루시퍼라제 시약(듀얼-글로(등록상표) 루시퍼라제 분석 시스템, 프로메가, 독일 만하임, 카탈로그 번호 E2980)을 사용하여 세포를 용해시키고, 반딧불이 및 레닐라 루시퍼라제를 제작자의 프로토콜에 따라 정량하였다. 레닐라 루시퍼라제 단백질 수준을 반딧불이 루시퍼라제 수준으로 표준화하였다. 각각의 dsRNA에 대해 12개의 개개 데이터 포인트를 3회의 독립적 실험들에서 수집하였다. 모든 표적 부위에 무관한 dsRNA를 dsRNA 처리된 세포에서 상대적 레닐라 루시퍼라제 단백질 수준을 측정하기 위한 대조군으로 사용하였다.
결과를 도 3, 4 및 5에 나타내었다.
PTP1B
를
표적화하는
dsRNA
의
생체내
효과(마우스 및
래트
)
마우스 간 조직에서 PTP1B mRNA 측정:
퀀티진 1.0 분지된 DNA(bDNA) 분석 키트(파노믹스(Panomics), 미국 캘리포니아 프리몬트, 카탈로그 번호 QG0004)를사용하여 간 조직으로부터 PTP1B mRNA 측정을 수행하였다.
부검시에, 1 내지 2 g의 간 조직을 액체 질소에서 순간 냉동시켰다. 냉동된 조직을 드라이 아이스 상에서 막자사발과 막자를 사용하여 분말화하였다. 15 내지 25 mg의 조직을 냉장된 1.5 ml 반응 튜브로 옮기고, 밀리큐 워터에 미리희석시킨 1:3 세포용해 혼합물 1 ml 및 3.3 ㎕의 프로테이나제 K(50 ㎍/㎕)를 가하고, 30 내지 50% 동력(HD2070, 반델린(Bandelin), 독일 베를린)에서 수초간 초음파 음파처리에 의해 조직을 용해시켰다. 세포용해물은 분석할 때까지 -80 ℃에서 보관하였다. mRNA 분석을 위해 세포용해물을 해동하고 1000 rpm 및 65 ℃에서(터모믹서 컴포트(Thermomixer comfort), 에펜도르프, 독일 함부르크) 15 분간 프로테이나제 K로 분해하였다. PTP1B 및 GAPDH mRNA 수준은 제작자의 권고에 따라 퀀티진 1.0 bDNA 부석 키트 시약을 사용하여 측정하였다. PTP1B 발현은 20 ㎕ 세포용해물을 사용하여 분석하였고 마우스/래트 PTP1B 프로브세트 및 GAPDH 발현은 40 ㎕ 세포용해물을 사용하여 분석하였으며, 래터스 노르베기커스 프로브세트는 마우스와 교차반응하는 것으로 나타났다(프로브세트의 배열 상기 참조). 분석 마지막에 화학발광 신호를 빅터 2 라이트 발광 계수기(Victor 2 Light luminescence counter; 퍼킨 엘머, 독일 바이스바덴)에서 상대 빛 단위(RLU)로 측정하였다. PTP1B 신호는 동일한 세포용해물 GAPDH 신호 및 GAPDH로 표준화된 PTP1B 발현으로 나타낸 값으로 나뉘었다.
dsRNA는 전술한 바와 같이 LNP01 중에 제형화되었다[Akinc, A. et al., Nature Biotech, 26(5):561-569 (2009)].
결과는 도 1 및 2에 나타내었다.
인슐린
신호전달에서
dsRNA
의 활성
HepG2 세포를 5nM dsRNA로 48 시간동안 형질감염시키고 24 시간동안 영양제한시키고 30 분간 인슐린으로 처리한 후 패스스캔 ELISA 분석을 행하였다. 절차에 대한 개요는 다음과 같다:
제 1 일: HepG2 세포 P8(ATCC MEME, 10% ATCC FBS, 1x l-gln)을 5nM dsRNA 및 다마펙트1(DharmalFECT1) 형질감염 시약으로 형질감염시켰다.
제 3 일: 세포를 dPBS로 1회 세척하고 영양제한 배지를 밤새 배양하는 동안 첨가하였다(ATCC MEME, 1x l-gln, 2% 스트립 혈청(stripped serum)).
제 4 일: 인슐린 자극후에 세포를 용해시켰다. 린(Lynn)으로부터 인슐린을 689 μM 농도로 수득하였다(인비트로겐 12585-014). 인슐린을 MEME(무첨가)중에 적절한 농도로 혼합하였다(예: 5 μM에서 10 ml이면 72.6 ㎕ 인슐린 및 9.9 ml MEME를 사용한다).
- 세포를 MEME로 1회 세척한 후 인슐린을 함유하는 MEME를 첨가한다.
- 세포를 37 ℃에서 인슐린과 함께 30 분간 배양한다.
- 세포를 빙냉시킨 dPBS로 1회 세척한다.
- dPBS를 제거하고 100 ㎕의 빙냉시킨 세포용해 완충액(1mM PMSF와 함께 1X CST #9803)을 각 웰에 가하고 얼음위에서 5 분간 배양한다
- 세포 플레이트를 냉동기에서 10 분간 교반하여 세포용해를 확실히 시킨 후 -80 ℃에서 보관한다.
세포 신호전달 기술 패스스캔 프소프-액트1(PathScan Phospho-Akt1) ELISA:
- 부하되는 대략적인 단백질(2 내지 5 ㎍)을 측정하기 위해 HepG2 세포용해물을 사용하여 초기에 희석 곡선을 수행한다.
- 10 ㎕ 세포용해물을 희석하고 ELISA 플레이트에 가한다. 이어서 CST 프로토콜을 수행한다.
- 3 ㎕ 단백질을 사용하여 마이크로BCA 분석을 실행하여 ELISA 결과를 각 웰에 첨가된 단백질로 표준화시킨다.
하기의 대조군들을 상기 실험에 사용하였다:
- Ctrl-10: 다마콘(Dharmacon)으로부터 리스크(Risc)-비함유 대조군(D-001220-01)
- Ctrl-11: 다마콘에 의해 합성된 일반적 대조군
- 모방체(Mock): dsRNA 비함유 형질감염 시약
결과는 도 6에 나타내었다.
SEQUENCE LISTING
<110> F. Hoffmann-La Roche AG
<120> COMPOSITIONS AND METHODS FOR INHIBITING EXPRESSION OF PTP1B GENES
<130> XXX
<140> PCT/EP2010/050814
<141> 2010-01-26
<150> EP 09151962.9
<151> 2009-02-03
<160> 1101
<210> 1
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 4, 6, 8, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 5, 7, 9, 10, 11, 12
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 1
augcauguaa ggucuucuut t 21
<210> 2
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 11, 13, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 2
aagaagaccu uacaugcaut t 21
<210> 3
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 5, 6, 13, 14, 15, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 7, 8, 9, 10, 11, 12, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 3
aaacucgaga gaucuuacat t 21
<210> 4
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 4
uguaagaucu cucgaguuut t 21
<210> 5
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 4, 14, 15, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 5
ugauggagaa agguucguut t 21
<210> 6
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 15, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 6
aacgaaccuu ucuccaucat t 21
<210> 7
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 10, 11, 12, 14, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 13, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 7
ggagaaaggu ucguuaaaat t 21
<210> 8
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 8
uuuuaacgaa ccuuucucct t 21
<210> 9
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 12, 13, 14, 15, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 16, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 9
aaaugaggaa guuucggaut t 21
<210> 10
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 10
auccgaaacu uccucauuut t 21
<210> 11
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 4, 5, 8, 10, 11, 14, 15, 16, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 6, 7, 9, 12, 13, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 11
auaucaacgc uaguuugaut t 21
<210> 12
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 8, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 9, 10, 11, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 12
aucaaacuag cguugauaut t 21
<210> 13
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 4, 9, 10, 16, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 5, 6, 7, 8, 11, 12, 13, 14, 15, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 13
cacugaaguu agaagucggt t 21
<210> 14
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 9, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 14
ccgacuucua acuucagugt t 21
<210> 15
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 11, 14, 15, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 15
cccuucuucc guugauauct t 21
<210> 16
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 6
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 16
gauaucaacg gaagaagggt t 21
<210> 17
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 7, 9, 10, 11, 14, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 6, 8, 12, 13, 15, 16, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 17
ucgucaugcu caacagagut t 21
<210> 18
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 12
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 18
acucuguuga gcaugacgat t 21
<210> 19
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 13, 14, 15, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 16, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 19
gauggagaaa gguucguuat t 21
<210> 20
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 20
uaacgaaccu uucuccauct t 21
<210> 21
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 5, 6, 8, 10, 11, 12, 13, 14, 16, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 7, 9, 15, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 21
gaucuuacau uuccacuaut t 21
<210> 22
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 13
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 22
auaguggaaa uguaagauct t 21
<210> 23
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 10, 11, 13, 16, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 8, 9, 12, 14, 15, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 23
ucccuuugac cauagucggt t 21
<210> 24
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 12
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 10, 11, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 24
ccgacuaugg ucaaagggat t 21
<210> 25
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 5, 6, 10, 11, 15, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 7, 8, 9, 12, 13, 14, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 25
auagucggau uaaacuacat t 21
<210> 26
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 8, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 9, 10, 11, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 26
uguaguuuaa uccgacuaut t 21
<210> 27
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 5, 6, 7, 8, 9, 12, 13, 15, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 10, 11, 14, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 27
cuaguucuca aucacugcut t 21
<210> 28
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 28
agcagugauu gagaacuagt t 21
<210> 29
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 6, 7, 8, 11, 12, 13, 14, 15, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 5, 9, 10, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 29
cuagaucuag uucucaauct t 21
<210> 30
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 11, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 30
gauugagaac uagaucuagt t 21
<210> 31
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 6, 12, 13, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 7, 8, 9, 10, 11, 14, 15, 16, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 31
gaaguuagaa gucgggucgt t 21
<210> 32
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 13
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 32
cgacccgacu ucuaacuuct t 21
<210> 33
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 5, 6, 9, 10, 11, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 7, 8, 12, 13
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 33
cguacugguu uaaccuccut t 21
<210> 34
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 8, 13, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 9, 10, 11, 12, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 34
aggagguuaa accaguacgt t 21
<210> 35
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 10, 14, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 6, 7, 8, 9, 11, 12, 13, 15, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 35
auuucaaaau ggacguacut t 21
<210> 36
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 9
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 36
aguacgucca uuuugaaaut t 21
<210> 37
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 6, 8, 10, 15, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 7, 9, 11, 12, 13, 14
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 37
gaaugcaugu aaggucuuct t 21
<210> 38
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 9, 11, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 10, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 38
gaagaccuua caugcauuct t 21
<210> 39
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 6, 8, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 5, 7, 9, 10, 11, 12, 15
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 39
caugauguga gauuacuuut t 21
<210> 40
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 11, 13, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 10, 12, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 40
aaaguaaucu cacaucaugt t 21
<210> 41
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 5, 11, 13, 14, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 6, 7, 8, 9, 10, 12, 15, 16
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 41
gguacagaga cgucagucct t 21
<210> 42
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 42
ggacugacgu cucuguacct t 21
<210> 43
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 4, 6, 8, 11, 13, 16, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 5, 7, 9, 10, 12, 14, 15, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 43
uauuauacag ugcgacagct t 21
<210> 44
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 8, 13, 15, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 9, 10, 11, 12, 14, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 44
gcugucgcac uguauaauat t 21
<210> 45
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 4, 5, 13, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 6, 7, 8, 9, 10, 11, 12, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 45
auguuagaga gguagcgagt t 21
<210> 46
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 14, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 46
cucgcuaccu cucuaacaut t 21
<210> 47
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 4, 6, 8, 10, 11, 12, 13, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 5, 7, 9, 14, 15, 16, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 47
ugcuauaugc cuuaagccat t 21
<210> 48
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 11, 13, 15, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 10, 12, 14, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 48
uggcuuaagg cauauagcat t 21
<210> 49
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 8, 9, 12, 14, 15, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 5, 6, 7, 10, 11, 13, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 49
ccuuaagcca auauuuacut t 21
<210> 50
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 7, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 50
aguaaauauu ggcuuaaggt t 21
<210> 51
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 9, 10, 11, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 5, 6, 7, 8, 12, 13, 14, 15, 16, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 51
uuucaaaguc cgagagucat t 21
<210> 52
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 52
ugacucucgg acuuugaaat t 21
<210> 53
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 11, 14, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 4, 5, 6, 7, 8, 9, 10, 12, 13, 15, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 53
guuagagagg uagcgagcut t 21
<210> 54
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 8, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 54
agcucgcuac cucucuaact t 21
<210> 55
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 9, 13, 14, 15, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 6, 7, 8, 10, 11, 12, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 55
cacccaaacg aauccuggat t 21
<210> 56
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 56
uccaggauuc guuugggugt t 21
<210> 57
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 13, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 57
gacccuucuu ccguugauat t 21
<210> 58
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 4
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 58
uaucaacgga agaaggguct t 21
<210> 59
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 4, 10, 12, 13, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 5, 6, 7, 8, 9, 11, 14, 15
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 59
guacagagac gucaguccct t 21
<210> 60
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 60
gggacugacg ucucuguact t 21
<210> 61
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 4, 6, 7, 10, 11, 12, 15, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 5, 8, 9, 13, 14
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 61
acguacuggu uuaaccucct t 21
<210> 62
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 7, 12, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 62
ggagguuaaa ccaguacgut t 21
<210> 63
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 9, 10, 11, 13, 14, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 5, 8, 12, 15, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 63
cccuguuauc ugcuagauct t 21
<210> 64
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 8, 12, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 9, 10, 11, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 64
gaucuagcag auaacagggt t 21
<210> 65
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 7, 9, 10, 11, 12, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 4, 5, 6, 8, 13, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 65
cucagacauu ccaaggguat t 21
<210> 66
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 66
uacccuugga augucugagt t 21
<210> 67
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 6, 10, 12, 13, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 5, 7, 8, 9, 11, 14, 15
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 67
ccggacggac guugguucut t 21
<210> 68
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 68
agaaccaacg uccguccggt t 21
<210> 69
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 6, 9, 10, 15, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 5, 7, 8, 11, 12, 13, 14, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 69
ugcgacagcu agaauuggat t 21
<210> 70
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 9, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 10, 11, 12, 13, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 70
uccaauucua gcugucgcat t 21
<210> 71
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 4, 8, 10, 11, 12, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 5, 6, 7, 9, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 71
acguggguau uuaauaagat t 21
<210> 72
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 7, 11, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 8, 9, 10, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 72
ucuuauuaaa uacccacgut t 21
<210> 73
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 4, 5, 7, 10, 11, 15, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 6, 8, 9, 12, 13, 14, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 73
ugaccauagu cggauuaaat t 21
<210> 74
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 12, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 74
uuuaauccga cuauggucat t 21
<210> 75
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 4, 7, 9, 10, 13, 14, 15, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 5, 6, 8, 11, 12, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 75
uaucaacgcu aguuugauat t 21
<210> 76
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 4, 9, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 5, 6, 7, 8, 10, 11, 12, 13, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 76
uaucaaacua gcguugauat t 21
<210> 77
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 11, 12, 13, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 7, 8, 9, 10, 14, 15, 16, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 77
cuuuucaaag uccgagagut t 21
<210> 78
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 78
acucucggac uuugaaaagt t 21
<210> 79
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 4, 6, 8, 9, 11, 13, 16, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 5, 7, 10, 12, 14, 15, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 79
cacuauacca cauggccugt t 21
<210> 80
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 6, 13, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 80
caggccaugu gguauagugt t 21
<210> 81
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 4, 7, 8, 10, 11, 13, 15, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 5, 6, 9, 12, 14, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 81
accugaucau uacauggcut t 21
<210> 82
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 8, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 82
agccauguaa ugaucaggut t 21
<210> 83
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 4, 7, 8, 9, 10, 11, 12, 14, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 5, 6, 13, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 83
cagugacuuc ccauguagat t 21
<210> 84
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 5, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 84
ucuacauggg aagucacugt t 21
<210> 85
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 6, 8, 9, 10, 11, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 7, 12, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 85
gagauuacuu ugucccgcut t 21
<210> 86
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 8, 13
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 86
agcgggacaa aguaaucuct t 21
<210> 87
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 5, 7, 8, 11, 12, 13, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 6, 9, 10, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 87
ucaacgcuag uuugauaaat t 21
<210> 88
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 6, 11
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 7, 8, 9, 10, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 88
uuuaucaaac uagcguugat t 21
<210> 89
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 5, 6, 8, 10, 13, 15, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 7, 9, 11, 12, 14, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 89
cauauuauac agugcgacat t 21
<210> 90
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 11, 13, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 10, 12, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 90
ugucgcacug uauaauaugt t 21
<210> 91
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 5, 6, 14, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 7, 8, 9, 10, 11, 12, 13, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 91
uauguuagag agguagcgat t 21
<210> 92
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 13, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 14, 15, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 92
ucgcuaccuc ucuaacauat t 21
<210> 93
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 4, 7, 9, 11, 12, 15, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 5, 6, 8, 10, 13, 14, 16
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 93
ccauagugca cuagcauuut t 21
<210> 94
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 7, 12, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 94
aaaugcuagu gcacuauggt t 21
<210> 95
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 9, 10, 11, 13, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 12, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 95
uugccuuucu cgugguagat t 21
<210> 96
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 6, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 96
ucuaccacga gaaaggcaat t 21
<210> 97
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 5, 6, 7, 8, 12, 13, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 9, 10, 11, 14, 15, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 97
uaugccuuaa gccaauauut t 21
<210> 98
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 11, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 98
aauauuggcu uaaggcauat t 21
<210> 99
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 4, 7, 8, 12, 13, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 5, 6, 9, 10, 11, 14, 15, 16, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 99
ccauagucgg auuaaacuat t 21
<210> 100
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 6, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 100
uaguuuaauc cgacuauggt t 21
<210> 101
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 12, 15, 16, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 101
acccuucuuc cguugauaut t 21
<210> 102
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 5
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 102
auaucaacgg aagaagggut t 21
<210> 103
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 6, 8, 14, 16, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 7, 9, 10, 11, 12, 13, 15, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 103
auagguacag agacgucagt t 21
<210> 104
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 13, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 104
cugacgucuc uguaccuaut t 21
<210> 105
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 12, 13, 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 8, 9, 10, 11, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 105
ucuuuucaaa guccgagagt t 21
<210> 106
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 106
cucucggacu uugaaaagat t 21
<210> 107
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 8, 10, 12, 13, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 4, 6, 7, 9, 11, 14, 15, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 107
uccauagugc acuagcauut t 21
<210> 108
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 11, 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 10, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 108
aaugcuagug cacuauggat t 21
<210> 109
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 4, 6, 7, 9, 13, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 5, 8, 10, 11, 12, 14, 15, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 109
ugauauugug gguaacgugt t 21
<210> 110
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 6, 10, 12, 15, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 7, 8, 9, 11, 13, 14, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 110
cacguuaccc acaauaucat t 21
<210> 111
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 11, 13, 14, 15, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 4, 7, 8, 9, 10, 12, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 111
uuuaccagga uauccgacat t 21
<210> 112
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 8, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 112
ugucggauau ccugguaaat t 21
<210> 113
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 10, 11, 12, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 6, 7, 8, 9, 13, 14, 15, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 113
uuuucaaagu ccgagaguct t 21
<210> 114
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 114
gacucucgga cuuugaaaat t 21
<210> 115
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 7, 8, 14, 15, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 5, 6, 9, 10, 11, 12, 13, 16, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 115
cugaaguuag aagucgggut t 21
<210> 116
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 11, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 116
acccgacuuc uaacuucagt t 21
<210> 117
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 7, 9, 10, 14, 15, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 4, 5, 6, 8, 11, 12, 13, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 117
uuugaguacc aaauccacat t 21
<210> 118
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 12, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 118
uguggauuug guacucaaat t 21
<210> 119
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 8, 15, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 9, 10, 11, 12, 13, 14, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 119
aaggguaugg gaagccauat t 21
<210> 120
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 11, 13
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 120
uauggcuucc cauacccuut t 21
<210> 121
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 4, 6, 8, 10, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 5, 7, 9, 11, 12, 15
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 121
uaacacaugc ggucacuuut t 21
<210> 122
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 11, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 122
aaagugaccg cauguguuat t 21
<210> 123
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 5, 6, 8, 13, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 7, 9, 10, 11, 12, 14, 15, 16, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 123
auggccaugg aauaaaccat t 21
<210> 124
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 11, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 10, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 124
ugguuuauuc cauggccaut t 21
<210> 125
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 9, 10, 13, 15, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 8, 11, 12, 14, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 125
uucuuccguu gauaucaagt t 21
<210> 126
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 9
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 126
cuugauauca acggaagaat t 21
<210> 127
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 5, 7, 8, 10, 14, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 6, 9, 11, 12, 13, 15, 16, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 127
gugauauugu ggguaacgut t 21
<210> 128
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 9, 11, 14, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 10, 12, 13, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 128
acguuaccca caauaucact t 21
<210> 129
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 7, 8, 12, 13, 15, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 6, 9, 10, 11, 14, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 129
uacucaucag gucauuauut t 21
<210> 130
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 130
aauaaugacc ugaugaguat t 21
<210> 131
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 7, 10, 12
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 5, 6, 8, 9, 11, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 131
uguggguaac gugagaagat t 21
<210> 132
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 7, 12, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 13, 14, 15, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 132
ucuucucacg uuacccacat t 21
<210> 133
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 5, 12, 13, 14, 15, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 6, 7, 8, 9, 10, 11, 16, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 133
aacucgagag aucuuacaut t 21
<210> 134
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 134
auguaagauc ucucgaguut t 21
<210> 135
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 5, 8, 9, 10, 13, 14, 16, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 6, 7, 11, 12, 15, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 135
ugcaugaccu gaucauuact t 21
<210> 136
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 9, 14, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 7, 8, 10, 11, 12, 13, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 136
guaaugauca ggucaugcat t 21
<210> 137
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 5, 8, 9, 10, 14, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 6, 7, 11, 12, 13, 15, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 137
guaguaguuu ggguguguat t 21
<210> 138
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 5, 9, 14, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 6, 7, 8, 10, 11, 12, 13, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 138
uacacaccca aacuacuact t 21
<210> 139
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 5, 6, 7, 10, 11, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 8, 9, 16
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 139
cugguuuaac cuccuaucct t 21
<210> 140
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 12, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 140
ggauaggagg uuaaaccagt t 21
<210> 141
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 5, 9, 12, 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 4, 6, 7, 8, 10, 11, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 141
auugugggua acgugagaat t 21
<210> 142
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 10, 14, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 11, 12, 13, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 142
uucucacguu acccacaaut t 21
<210> 143
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 10, 13, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 5, 6, 7, 8, 9, 11, 12, 14, 15, 16, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 143
uuagagaggu agcgagcugt t 21
<210> 144
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 9, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 144
cagcucgcua ccucucuaat t 21
<210> 145
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 6, 8, 9, 11, 15, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 7, 10, 12, 13, 14, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 145
agugauauug uggguaacgt t 21
<210> 146
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 8, 10, 13, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 9, 11, 12, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 146
cguuacccac aauaucacut t 21
<210> 147
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 13, 14, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 9, 10, 11, 12, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 147
uucuuuucaa aguccgagat t 21
<210> 148
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 148
ucucggacuu ugaaaagaat t 21
<210> 149
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 12, 13, 14, 16, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 15, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 149
auggagaaag guucguuaat t 21
<210> 150
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 150
uuaacgaacc uuucuccaut t 21
<210> 151
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 4, 11, 12, 13, 14, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 5, 6, 7, 8, 9, 10, 15, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 151
acucgagaga ucuuacauut t 21
<210> 152
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 152
aauguaagau cucucgagut t 21
<210> 153
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 4, 8, 9, 10, 13, 14, 15, 16, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 5, 6, 7, 11, 12, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 153
ugcuagaucu aguucucaat t 21
<210> 154
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 9, 15, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 13, 14, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 154
uugagaacua gaucuagcat t 21
<210> 155
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 6, 8, 10, 12, 15, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 4, 5, 7, 9, 11, 13, 14, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 155
ccuaacacau gcggucacut t 21
<210> 156
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 9, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 156
agugaccgca uguguuaggt t 21
<210> 157
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 6, 8, 9, 12, 13, 14, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 7, 10, 11, 15, 16
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 157
ggacguacug guuuaaccut t 21
<210> 158
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 10, 13
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 11, 12, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 158
agguuaaacc aguacgucct t 21
<210> 159
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 9, 10, 14, 15, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 4, 8, 11, 12, 13, 16, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 159
uuuacucauc aggucauuat t 21
<210> 160
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 160
uaaugaccug augaguaaat t 21
<210> 161
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 6, 7, 8, 9, 11, 12, 13, 14, 16, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 5, 10, 15, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 161
gauuacuuug ucccgcuuat t 21
<210> 162
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 10, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 162
uaagcgggac aaaguaauct t 21
<210> 163
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 8, 9, 13, 14, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 5, 6, 7, 10, 11, 12, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 163
uuagaagucg ggucgugggt t 21
<210> 164
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 164
cccacgaccc gacuucuaat t 21
<210> 165
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 5, 9, 11, 13, 14, 16, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 6, 7, 8, 10, 12, 15, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 165
cuagcaggca ugccgcggut t 21
<210> 166
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 8, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 9, 10, 11, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 166
accgcggcau gccugcuagt t 21
<210> 167
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 5, 6, 8, 12, 15, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 7, 9, 10, 11, 13, 14, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 167
gauauugugg guaacgugat t 21
<210> 168
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 7, 11, 13, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 8, 9, 10, 12, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 168
ucacguuacc cacaauauct t 21
<210> 169
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 4, 6, 7, 9, 11, 14, 16, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 5, 8, 10, 12, 13, 15, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 169
ucauauuaua cagugcgact t 21
<210> 170
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 10, 12, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 11, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 170
gucgcacugu auaauaugat t 21
<210> 171
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 6, 7, 8, 9, 11, 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 10, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 171
gccuuucucg ugguagaagt t 21
<210> 172
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 8
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 172
cuucuaccac gagaaaggct t 21
<210> 173
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 9, 11, 13, 15, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 7, 8, 10, 12, 14, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 173
uugccuaaca caugcgguct t 21
<210> 174
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 13, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 174
gaccgcaugu guuaggcaat t 21
<210> 175
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 8, 10, 11, 15, 16, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 5, 6, 7, 9, 12, 13, 14, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 175
ucgaaggugc caaauucaut t 21
<210> 176
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 11
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 176
augaauuugg caccuucgat t 21
<210> 177
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 4, 7, 11, 13, 15, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 5, 6, 8, 9, 10, 12, 14, 16, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 177
auauaaugaa cacgugggut t 21
<210> 178
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 12, 15, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 13, 14, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 178
acccacgugu ucauuauaut t 21
<210> 179
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 5, 8, 9, 13, 14, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 4, 6, 7, 10, 11, 12, 15, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 179
accauagucg gauuaaacut t 21
<210> 180
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 180
aguuuaaucc gacuauggut t 21
<210> 181
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 10, 11, 13, 15, 16, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 5, 6, 7, 8, 9, 12, 14, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 181
uaugggaagc cauauucact t 21
<210> 182
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 182
gugaauaugg cuucccauat t 21
<210> 183
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 5, 6, 7, 8, 10, 11, 12, 13, 14, 15, 16, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 9, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 183
cacauuuugc cuuucucgut t 21
<210> 184
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 11
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 184
acgagaaagg caaaaugugt t 21
<210> 185
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 5, 8, 9, 10, 11, 12, 15, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 6, 7, 13, 14, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 185
gaucuaguuc ucaaucacut t 21
<210> 186
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 186
agugauugag aacuagauct t 21
<210> 187
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 4, 5, 6, 9, 10, 12, 13, 15, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 7, 8, 11, 14, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 187
ugaccugauc auuacauggt t 21
<210> 188
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 6, 13, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 188
ccauguaaug aucaggucat t 21
<210> 189
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 5, 6, 7, 9, 11, 12, 13, 14, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 8, 10, 15, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 189
cauauucaca ccucacgcut t 21
<210> 190
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 190
agcgugaggu gugaauaugt t 21
<210> 191
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 7, 8, 10, 11, 12, 13, 15, 16, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 9, 14, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 191
gugagauuac uuugucccgt t 21
<210> 192
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 11, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 10, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 192
cgggacaaag uaaucucact t 21
<210> 193
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 5, 6, 9, 13, 14, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 7, 8, 10, 11, 12, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 193
cagaucgaca aguccgggat t 21
<210> 194
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 194
ucccggacuu gucgaucugt t 21
<210> 195
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 4, 5, 6, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 7, 8, 15
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 195
ugguuuaacc uccuauccut t 21
<210> 196
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 13, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 196
aggauaggag guuaaaccat t 21
<210> 197
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 5, 6, 7, 8, 9, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 10, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 197
aagcuuccua agaacaaaat t 21
<210> 198
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 10
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 198
uuuuguucuu aggaagcuut t 21
<210> 199
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 11, 15, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 7, 8, 9, 10, 12, 13, 14, 16, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 199
cauuucaaaa uggacguact t 21
<210> 200
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 8
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 7, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 200
guacguccau uuugaaaugt t 21
<210> 201
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 9, 10, 14, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 6, 8, 11, 12, 13, 15, 16
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 201
ccuccacauu aaguggcuut t 21
<210> 202
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 9
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 202
aagccacuua auguggaggt t 21
<210> 203
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 5, 7, 10, 13, 14, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 6, 8, 9, 11, 12, 15, 16, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 203
acagugcgac agcuagaaut t 21
<210> 204
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 204
auucuagcug ucgcacugut t 21
<210> 205
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 8, 9, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 5, 6, 7, 10, 15
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 205
ugugagauua cuuuguccct t 21
<210> 206
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 10, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 11, 12, 13, 14, 15, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 206
gggacaaagu aaucucacat t 21
<210> 207
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 7, 8, 10, 13, 14, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 5, 6, 9, 11, 12, 15, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 207
cuuugaccau agucggauut t 21
<210> 208
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 9, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 208
aauccgacua uggucaaagt t 21
<210> 209
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 4, 6, 7, 11, 12, 13, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 5, 8, 9, 10, 14, 15
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 209
aucugcuaga ucuaguucut t 21
<210> 210
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 12, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 10, 11, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 210
agaacuagau cuagcagaut t 21
<210> 211
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 7, 9, 10, 13, 14, 15, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 8, 11, 12, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 211
gacggacguu gguucugcat t 21
<210> 212
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 9
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 212
ugcagaacca acguccguct t 21
<210> 213
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 5, 7, 8, 13, 15, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 6, 9, 10, 11, 12, 14, 16, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 213
gguucguuaa aaugcgcact t 21
<210> 214
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 11
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 10, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 214
gugcgcauuu uaacgaacct t 21
<210> 215
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 7, 9, 11, 12, 13, 14, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 6, 8, 10, 15, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 215
cugcuauaug ccuuaagcct t 21
<210> 216
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 10, 12, 14, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 11, 13, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 216
ggcuuaaggc auauagcagt t 21
<210> 217
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 9, 13, 15, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 5, 6, 7, 8, 10, 11, 12, 14, 16, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 217
uuucaaaaug gacguacugt t 21
<210> 218
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 4, 10
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 5, 6, 7, 8, 9, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 218
caguacgucc auuuugaaat t 21
<210> 219
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 5, 7, 8, 9, 10, 14, 15, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 6, 11, 12, 13, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 219
uauaugccuu aagccaauat t 21
<210> 220
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 9, 14, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 13, 15, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 220
uauuggcuua aggcauauat t 21
<210> 221
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 6, 9, 10, 14, 15, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 5, 7, 8, 11, 12, 13, 16, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 221
gaccauaguc ggauuaaact t 21
<210> 222
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 13
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 222
guuuaauccg acuaugguct t 21
<210> 223
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 6, 7, 8, 15, 16, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 5, 9, 10, 11, 12, 13, 14, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 223
uacaacccaa gaaacucgat t 21
<210> 224
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 224
ucgaguuucu uggguuguat t 21
<210> 225
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 5, 7, 9, 11, 14, 15, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 6, 8, 10, 12, 13, 16
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 225
cuaacacaug cggucacuut t 21
<210> 226
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 10, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 226
aagugaccgc auguguuagt t 21
<210> 227
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 5, 6, 8, 9, 10, 14, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 7, 11, 12, 13, 15, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 227
ccggccaccc aaacgaauct t 21
<210> 228
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 228
gauucguuug gguggccggt t 21
<210> 229
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 6, 7, 9, 10, 11, 12, 14, 15, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 8, 13, 16, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 229
gcgagcugcu cugcuauaut t 21
<210> 230
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 4, 7, 12
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 5, 6, 8, 9, 10, 11, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 230
auauagcaga gcagcucgct t 21
<210> 231
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 5, 6, 7, 8, 10, 11, 12, 13, 15, 16, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 4, 9, 14, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 231
auuacuuugu cccgcuuaut t 21
<210> 232
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 11, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 232
auaagcggga caaaguaaut t 21
<210> 233
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 4, 6, 7, 8, 10, 12, 13, 14, 15, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 5, 9, 11, 16, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 233
ccauauucac accucacgct t 21
<210> 234
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 234
gcgugaggug ugaauauggt t 21
<210> 235
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 4, 6, 7, 11, 12, 14, 15, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 5, 8, 9, 10, 13, 16
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 235
acucaucagg ucauuauuut t 21
<210> 236
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 236
aaauaaugac cugaugagut t 21
<210> 237
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 5, 7, 8, 9, 11, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 6, 10, 12, 13
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 237
auggugcuca cgacucuuct t 21
<210> 238
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 14, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 238
gaagagucgu gagcaccaut t 21
<210> 239
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 9, 10, 13, 15, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 4, 8, 11, 12, 14, 16
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 239
ucugcuuacu aaugugccct t 21
<210> 240
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 6, 9, 12, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 7, 8, 10, 11, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 240
gggcacauua guaagcagat t 21
<210> 241
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 5, 7, 8, 9, 10, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 6, 11, 16
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 241
agauuacuuu gucccgcuut t 21
<210> 242
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 9, 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 242
aagcgggaca aaguaaucut t 21
<210> 243
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 6, 10, 11, 12, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 4, 5, 7, 8, 9, 13, 14, 15, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 243
aucgacaagu ccgggagcut t 21
<210> 244
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 244
agcucccgga cuugucgaut t 21
<210> 245
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 11, 16, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 245
gcaacucucc acuccauaut t 21
<210> 246
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 246
auauggagug gagaguugct t 21
<210> 247
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 9, 10, 11, 12, 14, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 5, 6, 7, 8, 13, 15
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 247
ucgagagauc uuacauuuct t 21
<210> 248
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 7
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 248
gaaauguaag aucucucgat t 21
<210> 249
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 5, 6, 9, 13, 15, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 7, 8, 10, 11, 12, 14, 16, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 249
ccgacuagca ggcaugccgt t 21
<210> 250
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 13
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 250
cggcaugccu gcuagucggt t 21
<210> 251
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 6, 7, 9, 10, 11, 12, 14, 15, 16, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 8, 13, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 251
ugagauuacu uugucccgct t 21
<210> 252
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 7, 12, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 13, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 252
gcgggacaaa guaaucucat t 21
<210> 253
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 7, 8, 12, 13, 15, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 4, 5, 6, 9, 10, 11, 14, 16, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 253
gucggauuaa acuacaucat t 21
<210> 254
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 11
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 10, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 254
ugauguaguu uaauccgact t 21
<210> 255
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 4, 7, 9, 11, 13, 16, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 5, 6, 8, 10, 12, 14, 15, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 255
gccuaacaca ugcggucact t 21
<210> 256
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 8, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 256
gugaccgcau guguuaggct t 21
<210> 257
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 4, 6, 7, 8, 10, 11, 15, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 5, 9, 12, 13, 14, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 257
auuuacucau caggucauut t 21
<210> 258
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 258
aaugaccuga ugaguaaaut t 21
<210> 259
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 9, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 10, 11, 12, 15
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 259
agagguagcg agcugcucut t 21
<210> 260
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 13
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 260
agagcagcuc gcuaccucut t 21
<210> 261
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 6, 8, 9, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 7, 10, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 261
gguaguacug ggaauggaat t 21
<210> 262
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 10, 13, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 8, 9, 11, 12, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 262
uuccauuccc aguacuacct t 21
<210> 263
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 4, 6, 8, 9, 10, 11, 13, 15, 16, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 5, 7, 12, 14, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 263
auucacaccu cacgcucugt t 21
<210> 264
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 264
cagagcguga ggugugaaut t 21
<210> 265
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 5, 7, 9, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 6, 8, 10, 11, 14
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 265
aacacaugcg gucacuuuut t 21
<210> 266
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 12
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 266
aaaagugacc gcauguguut t 21
<210> 267
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 5, 7, 8, 12, 13, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 4, 6, 9, 10, 11, 14, 15, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 267
gcugcgucag accaguacut t 21
<210> 268
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 268
aguacugguc ugacgcagct t 21
<210> 269
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 10, 11, 13, 15, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 6, 7, 8, 9, 12, 14, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 269
auccuggagc cacacaaugt t 21
<210> 270
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 270
cauugugugg cuccaggaut t 21
<210> 271
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 9, 10, 12, 13, 14, 15, 16, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 6, 7, 8, 11, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 271
cucccgagcu acucucuugt t 21
<210> 272
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 9
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 272
caagagagua gcucgggagt t 21
<210> 273
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 6, 8, 9, 13, 14, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 5, 7, 10, 11, 12, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 273
uuaggugcca ggcuguaagt t 21
<210> 274
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 5, 13, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 274
cuuacagccu ggcaccuaat t 21
<210> 275
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 4, 8, 11, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 5, 6, 7, 9, 10, 12, 13
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 275
gccuggguga cgguccuuut t 21
<210> 276
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 11, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 276
aaaggaccgu cacccaggct t 21
<210> 277
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 10, 11, 15, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 5, 6, 7, 8, 9, 12, 13, 14, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 277
aguuagaagu cgggucgugt t 21
<210> 278
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 278
cacgacccga cuucuaacut t 21
<210> 279
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 279
gaaagacccu ucuuccguut t 21
<210> 280
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 280
aacggaagaa gggucuuuct t 21
<210> 281
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 4, 6, 8, 11, 12, 14, 15, 16, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 5, 7, 9, 10, 13, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 281
acacaugcgg ucacuuuugt t 21
<210> 282
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 13
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 282
caaaagugac cgcaugugut t 21
<210> 283
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 8, 12, 14, 16, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 4, 5, 6, 7, 9, 10, 11, 13, 15, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 283
uucaaaaugg acguacuggt t 21
<210> 284
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 5, 11
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 6, 7, 8, 9, 10, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 284
ccaguacguc cauuuugaat t 21
<210> 285
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 8, 9, 11, 14, 15, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 6, 7, 10, 12, 13, 16, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 285
ccuuugacca uagucggaut t 21
<210> 286
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 8, 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 9, 10, 11, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 286
auccgacuau ggucaaaggt t 21
<210> 287
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 6, 7, 8, 9, 10, 11, 12, 16, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 13, 14, 15, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 287
ggaguucuuc ccaaaucact t 21
<210> 288
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 288
gugauuuggg aagaacucct t 21
<210> 289
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 4, 6, 7, 12, 14, 15, 16, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 5, 8, 9, 10, 11, 13, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 289
auuuaccagg auauccgact t 21
<210> 290
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 7, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 290
gucggauauc cugguaaaut t 21
<210> 291
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 6, 7, 13, 14, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 8, 9, 10, 11, 12, 15, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 291
ugaaguuaga agucggguct t 21
<210> 292
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 12, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 292
gacccgacuu cuaacuucat t 21
<210> 293
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 8, 10, 11, 13, 15, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 4, 7, 9, 12, 14, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 293
uccacuauac cacauggcct t 21
<210> 294
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 11, 13
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 8, 9, 10, 12, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 294
ggccaugugg uauaguggat t 21
<210> 295
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 5, 15, 16, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 295
gugauggaga aagguucgut t 21
<210> 296
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 14, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 296
acgaaccuuu cuccaucact t 21
<210> 297
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 7, 9, 13, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 8, 10, 11, 12, 14, 15, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 297
gugaggugug gauaaggcut t 21
<210> 298
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 10, 12, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 11, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 298
agccuuaucc acaccucact t 21
<210> 299
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 6, 7, 8, 9, 11, 13, 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 4, 5, 10, 12, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 299
gucagccuug caucaagggt t 21
<210> 300
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 10
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 300
cccuugaugc aaggcugact t 21
<210> 301
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 5, 8, 9, 10, 11, 12, 13, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 6, 7, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 301
gugacggucc uuuaggcagt t 21
<210> 302
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 302
cugccuaaag gaccgucact t 21
<210> 303
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 5, 6, 8, 10, 13, 14, 15, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 7, 9, 11, 12, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 303
uauaccacau ggccugacut t 21
<210> 304
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 9, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 8, 10, 11, 12, 13, 14, 15, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 304
agucaggcca ugugguauat t 21
<210> 305
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 7, 9, 10, 11, 12, 14, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 6, 8, 13, 15
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 305
uauucacacc ucacgcucut t 21
<210> 306
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 306
agagcgugag gugugaauat t 21
<210> 307
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 6, 9, 10, 11, 12, 13, 14, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 7, 8, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 307
ggugacgguc cuuuaggcat t 21
<210> 308
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 308
ugccuaaagg accgucacct t 21
<210> 309
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 4, 5, 8, 9, 10, 11, 13, 15, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 6, 7, 12, 14, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 309
augucagccu ugcaucaagt t 21
<210> 310
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 8, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 9, 10, 11, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 310
cuugaugcaa ggcugacaut t 21
<210> 311
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 9, 11, 14, 16, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 4, 5, 6, 7, 8, 10, 12, 13, 15, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 311
gcuaagagcg cgacgcggct t 21
<210> 312
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 312
gccgcgucgc gcucuuagct t 21
<210> 313
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 9, 11, 12, 14, 16, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 5, 8, 10, 13, 15, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 313
uuccacuaua ccacauggct t 21
<210> 314
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 10, 12
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 9, 11, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 314
gccauguggu auaguggaat t 21
<210> 315
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 4, 5, 7, 9, 12, 14, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 6, 8, 10, 11, 13, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 315
auauuauaca gugcgacagt t 21
<210> 316
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 7, 12, 14, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 13, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 316
cugucgcacu guauaauaut t 21
<210> 317
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 8, 9, 13, 14, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 5, 6, 7, 10, 11, 12, 15, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 317
agucggauua aacuacauct t 21
<210> 318
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 10
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 318
gauguaguuu aauccgacut t 21
<210> 319
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 5, 6, 7, 9, 11, 12, 13, 14, 15, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 8, 10, 16, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 319
agaucuuaca uuuccacuat t 21
<210> 320
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 12
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 320
uaguggaaau guaagaucut t 21
<210> 321
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 6, 8, 9, 10, 12, 15, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 7, 11, 13, 14
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 321
gauggugcuc acgacucuut t 21
<210> 322
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 13, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 322
aagagucgug agcaccauct t 21
<210> 323
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 9, 12, 13, 14, 16, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 6, 7, 8, 10, 11, 15, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 323
ccuuuaggca gccugccgct t 21
<210> 324
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 324
gcggcaggcu gccuaaaggt t 21
<210> 325
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 5, 6, 7, 9, 11, 12, 16, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 8, 10, 13, 14, 15, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 325
gaccuccaca uuaaguggct t 21
<210> 326
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 7
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 326
gccacuuaau guggagguct t 21
<210> 327
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 15, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 327
uuauucuccu cccuguuaut t 21
<210> 328
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 5, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 328
auaacaggga ggagaauaat t 21
<210> 329
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 5, 7, 8, 11, 12, 13, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 6, 9, 10, 14, 15
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 329
gacguacugg uuuaaccuct t 21
<210> 330
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 11, 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 10, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 330
gagguuaaac caguacguct t 21
<210> 331
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 8, 9, 10, 11, 12, 13, 14, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 331
gaaggagcuu ucccacgagt t 21
<210> 332
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 332
cucgugggaa agcuccuuct t 21
<210> 333
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 7, 8, 9, 11, 14, 15, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 10, 12, 13, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 333
gaaaaccuua caacccaagt t 21
<210> 334
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 10
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 334
cuuggguugu aagguuuuct t 21
<210> 335
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 5, 6, 7, 8, 9, 13, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 10, 11, 12, 14, 15, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 335
uaaauccuca gguaguacut t 21
<210> 336
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 6, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 336
aguacuaccu gaggauuuat t 21
<210> 337
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 5, 8, 9, 10, 11, 12, 14, 15, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 6, 7, 13, 16
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 337
ggaucagccu ccgccauuct t 21
<210> 338
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 338
gaauggcgga ggcugaucct t 21
<210> 339
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 5, 7, 8, 9, 10, 12, 13
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 6, 11, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 339
gguucaccuu gccgagagat t 21
<210> 340
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 9
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 340
ucucucggca aggugaacct t 21
<210> 341
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 4, 7, 9, 10, 11, 13, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 5, 6, 8, 12, 14, 15
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 341
ugauggugcu cacgacucut t 21
<210> 342
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 12, 15, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 342
agagucguga gcaccaucat t 21
<210> 343
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 5, 6, 7, 8, 9, 11, 12, 14, 16, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 10, 13, 15, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 343
uacauuucca cuauaccact t 21
<210> 344
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 7, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 344
gugguauagu ggaaauguat t 21
<210> 345
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 4, 7, 8, 9, 12, 13, 14, 15, 16, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 5, 6, 10, 11, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 345
uacugguuua accuccuaut t 21
<210> 346
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 10, 15, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 346
auaggagguu aaaccaguat t 21
<210> 347
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 6, 7, 10, 14, 15, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 8, 9, 11, 12, 13, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 347
gcagaucgac aaguccgggt t 21
<210> 348
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 348
cccggacuug ucgaucugct t 21
<210> 349
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 6, 8, 10, 11, 13, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 7, 9, 12, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 349
gcaggcaugc cgcgguaggt t 21
<210> 350
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 11
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 350
ccuaccgcgg caugccugct t 21
<210> 351
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 4, 6, 7, 9, 12, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 5, 8, 10, 11, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 351
gcaugccgcg guagguaagt t 21
<210> 352
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 7, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 352
cuuaccuacc gcggcaugct t 21
<210> 353
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 5, 6, 8, 11, 12, 16, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 7, 9, 10, 13, 14, 15, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 353
uugaccauag ucggauuaat t 21
<210> 354
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 11, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 354
uuaauccgac uauggucaat t 21
<210> 355
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 4, 5, 7, 9, 12, 13, 14, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 6, 8, 10, 11, 15, 16
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 355
auaccacaug gccugacuut t 21
<210> 356
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 10, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 11, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 356
aagucaggcc augugguaut t 21
<210> 357
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 6, 7, 9, 12, 13, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 4, 5, 8, 10, 11, 14, 15, 16, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 357
uuugaccaua gucggauuat t 21
<210> 358
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 10, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 358
uaauccgacu auggucaaat t 21
<210> 359
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 16, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 359
aaagacccuu cuuccguugt t 21
<210> 360
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 360
caacggaaga agggucuuut t 21
<210> 361
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 5, 6, 7, 10, 11, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 8, 9, 12, 15
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 361
aguucucaau cacugcucct t 21
<210> 362
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 362
ggagcaguga uugagaacut t 21
<210> 363
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 6, 7, 11, 12, 14, 16, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 5, 8, 9, 10, 13, 15, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 363
ucggauuaaa cuacaucaat t 21
<210> 364
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 7, 12
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 364
uugauguagu uuaauccgat t 21
<210> 365
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 9, 11, 12, 15, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 5, 6, 7, 8, 10, 13, 14
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 365
uacagagacg ucagucccut t 21
<210> 366
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 366
agggacugac gucucuguat t 21
<210> 367
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 6, 8, 11, 12, 13, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 5, 7, 9, 10, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 367
cgcgacgcgg ccuagagcgt t 21
<210> 368
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 368
cgcucuaggc cgcgucgcgt t 21
<210> 369
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 6, 7, 9, 10, 15, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 8, 11, 12, 13, 14, 16, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 369
aagguucguu aaaaugcgct t 21
<210> 370
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 9
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 8, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 370
gcgcauuuua acgaaccuut t 21
<210> 371
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 5, 6, 7, 8, 9, 10, 14, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 11, 12, 13, 15, 16
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 371
acgguccuuu aggcagccut t 21
<210> 372
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 9
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 372
aggcugccua aaggaccgut t 21
<210> 373
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 5, 7, 8, 12, 13, 15, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 6, 9, 10, 11, 14, 16, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 373
uaggugccag gcuguaagct t 21
<210> 374
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 6, 14, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 7, 8, 9, 10, 11, 12, 13, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 374
gcuuacagcc uggcaccuat t 21
<210> 375
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 5, 7, 10, 12, 15, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 4, 6, 8, 9, 11, 13, 14, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 375
auuauacagu gcgacagcut t 21
<210> 376
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 9, 14, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 13, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 376
agcugucgca cuguauaaut t 21
<210> 377
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 4, 7, 8, 9, 10, 11, 14, 15, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 5, 6, 12, 13, 16, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 377
aucuaguucu caaucacugt t 21
<210> 378
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 378
cagugauuga gaacuagaut t 21
<210> 379
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 7, 9, 10, 12, 14, 15, 16, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 4, 5, 6, 8, 11, 13, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 379
cuuaaaugcc gcacccuact t 21
<210> 380
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 12, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 380
guagggugcg gcauuuaagt t 21
<210> 381
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 5, 6, 8, 9, 14, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 7, 10, 11, 12, 13, 15, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 381
agguucguua aaaugcgcat t 21
<210> 382
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 10
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 382
ugcgcauuuu aacgaaccut t 21
<210> 383
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 4, 6, 8, 11, 12, 13, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 5, 7, 9, 10, 14, 15
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 383
caacacauag ccugacccut t 21
<210> 384
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 11
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 10, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 384
agggucaggc uauguguugt t 21
<210> 385
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 6, 7, 8, 10, 11, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 9, 12, 15
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 385
ggcagccugc cgccgucuct t 21
<210> 386
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 11
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 386
gagacggcgg caggcugcct t 21
<210> 387
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 6, 7, 14, 15, 16, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 8, 9, 10, 11, 12, 13, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 387
gaaacucgag agaucuuact t 21
<210> 388
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 388
guaagaucuc ucgaguuuct t 21
<210> 389
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 4, 7, 8, 9, 10, 11, 15, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 5, 6, 12, 13, 14, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 389
cuguagcucc cgagcuacut t 21
<210> 390
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 15, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 390
aguagcucgg gagcuacagt t 21
<210> 391
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 5, 6, 8, 11, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 7, 9, 10, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 391
caugccgcgg uagguaaggt t 21
<210> 392
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 8, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 392
ccuuaccuac cgcggcaugt t 21
<210> 393
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 5, 6, 7, 8, 9, 11, 12, 13, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 10, 14, 15, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 393
gggcccuuug ccuaacacat t 21
<210> 394
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 10
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 394
uguguuaggc aaagggccct t 21
<210> 395
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 7, 8, 9, 10, 12, 14, 15, 16, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 4, 6, 11, 13, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 395
uucacaccuc acgcucuggt t 21
<210> 396
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 396
ccagagcgug aggugugaat t 21
<210> 397
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 4, 5, 6, 7, 8, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 9, 10, 11, 14
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 397
uagcucccga gcuacucuct t 21
<210> 398
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 398
gagaguagcu cgggagcuat t 21
<210> 399
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 4, 5, 7, 10, 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 6, 8, 9, 11, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 399
augccgcggu agguaagggt t 21
<210> 400
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 9, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 10, 11, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 400
cccuuaccua ccgcggcaut t 21
<210> 401
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 6, 7, 11, 12, 16, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 5, 8, 9, 10, 13, 14, 15, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 401
cauagucgga uuaaacuact t 21
<210> 402
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 7, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 402
guaguuuaau ccgacuaugt t 21
<210> 403
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 4, 6, 9, 13, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 5, 7, 8, 10, 11, 12, 14, 15, 16, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 403
ugccgcggua gguaagggct t 21
<210> 404
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 10, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 11, 12, 13, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 404
gcccuuaccu accgcggcat t 21
<210> 405
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 5, 7, 10, 11, 12, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 6, 8, 9, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 405
gcgacgcggc cuagagcggt t 21
<210> 406
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 7
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 406
ccgcucuagg ccgcgucgct t 21
<210> 407
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 5, 6, 7, 9, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 8, 10, 11
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 407
ggugcucacg acucuuccut t 21
<210> 408
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 408
aggaagaguc gugagcacct t 21
<210> 409
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 6, 10, 12, 14, 15, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 4, 5, 7, 8, 9, 11, 13, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 409
acuagcaggc augccgcggt t 21
<210> 410
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 7, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 410
ccgcggcaug ccugcuagut t 21
<210> 411
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 8, 10, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 9, 11, 12, 13, 14, 15, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 411
aaauagguac agagacguct t 21
<210> 412
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 11, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 412
gacgucucug uaccuauuut t 21
<210> 413
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 8, 9, 10, 14, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 11, 12, 13, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 413
gaaacagccu gggugacggt t 21
<210> 414
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 9
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 414
ccgucaccca ggcuguuuct t 21
<210> 415
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 7, 9, 11, 12, 14, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 8, 10, 13, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 415
agcaggcaug ccgcgguagt t 21
<210> 416
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 10
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 416
cuaccgcggc augccugcut t 21
<210> 417
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 5, 6, 7, 9, 10, 15, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 4, 8, 11, 12, 13, 14, 16
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 417
gccauuuacc aggauaucct t 21
<210> 418
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 12
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 418
ggauauccug guaaauggct t 21
<210> 419
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 4, 7, 9, 12, 13, 14, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 5, 6, 8, 10, 11, 15, 16, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 419
gcgcgacgcg gccuagagct t 21
<210> 420
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 420
gcucuaggcc gcgucgcgct t 21
<210> 421
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 8, 10, 12, 14, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 6, 7, 9, 11, 13, 15, 16, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 421
ugccuaacac augcggucat t 21
<210> 422
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 7, 14, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 12, 13, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 422
ugaccgcaug uguuaggcat t 21
<210> 423
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 4, 5, 7, 8, 9, 13, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 6, 10, 11, 12, 14, 15, 16
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 423
cggccaccca aacgaaucct t 21
<210> 424
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 424
ggauucguuu ggguggccgt t 21
<210> 425
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 7, 8, 11, 12, 13, 14, 15, 16, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 9, 10, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 425
gagacgucag ucccuuugat t 21
<210> 426
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 426
ucaaagggac ugacgucuct t 21
<210> 427
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 5, 7, 8, 10, 13, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 6, 9, 11, 12, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 427
ggcaugccgc gguagguaat t 21
<210> 428
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 6, 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 428
uuaccuaccg cggcaugcct t 21
<210> 429
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 5, 8, 10, 13, 14, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 6, 7, 9, 11, 12, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 429
agcgcgacgc ggccuagagt t 21
<210> 430
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 430
cucuaggccg cgucgcgcut t 21
<210> 431
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 6, 7, 8, 9, 15, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 10, 11, 12, 13, 14, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 431
agacauucca aggguauggt t 21
<210> 432
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 4
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 432
ccauacccuu ggaaugucut t 21
<210> 433
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 7, 9, 10, 12, 14, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 6, 8, 11, 13, 15, 16
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 433
ccacuauacc acauggccut t 21
<210> 434
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 12, 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 434
aggccaugug guauaguggt t 21
<210> 435
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 9, 10, 12, 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 6, 7, 8, 11, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 435
ccgccggacc gcguagagat t 21
<210> 436
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 436
ucucuacgcg guccggcggt t 21
<210> 437
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 14, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 4, 13, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 437
uuugccuuuc ucgugguagt t 21
<210> 438
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 5, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 438
cuaccacgag aaaggcaaat t 21
<210> 439
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 5, 6, 7, 8, 9, 10, 11, 15, 16, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 12, 13, 14, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 439
gaguucuucc caaaucacct t 21
<210> 440
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 440
ggugauuugg gaagaacuct t 21
<210> 441
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 6, 7, 8, 9, 10, 11, 13, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 12, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 441
agugacuucc cauguagagt t 21
<210> 442
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 6, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 442
cucuacaugg gaagucacut t 21
<210> 443
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 5, 6, 7, 11, 15, 16, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 4, 8, 9, 10, 12, 13, 14, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 443
gccacccaaa cgaauccugt t 21
<210> 444
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 444
caggauucgu uuggguggct t 21
<210> 445
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 5, 7, 9, 10, 11, 14, 15, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 6, 8, 12, 13, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 445
agcccacgcc cgacuagcat t 21
<210> 446
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 446
ugcuagucgg gcgugggcut t 21
<210> 447
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 5, 7, 9, 10, 12, 15, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 6, 8, 11, 13, 14, 16, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 447
caggcaugcc gcgguaggut t 21
<210> 448
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 12
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 448
accuaccgcg gcaugccugt t 21
<210> 449
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 6, 7, 8, 12, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 5, 9, 10, 11, 13, 14, 15
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 449
ggccacccaa acgaauccut t 21
<210> 450
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 450
aggauucguu uggguggcct t 21
<210> 451
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 5, 6, 8, 10, 11, 12, 15, 16, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 7, 9, 13, 14, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 451
gagcccacgc ccgacuagct t 21
<210> 452
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 452
gcuagucggg cgugggcuct t 21
<210> 453
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 9, 10, 15, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 11, 12, 13, 14, 16, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 453
aacaaaaacc gaaauaggut t 21
<210> 454
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 454
accuauuucg guuuuuguut t 21
<210> 455
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 4, 5, 8, 12, 14, 16, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 6, 7, 9, 10, 11, 13, 15, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 455
cgacuagcag gcaugccgct t 21
<210> 456
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 456
gcggcaugcc ugcuagucgt t 21
<210> 457
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 5, 11, 12, 14, 15, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 6, 7, 8, 9, 10, 13, 16, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 457
uagguaaggg ccgccggact t 21
<210> 458
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 14, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 458
guccggcggc ccuuaccuat t 21
<210> 459
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 8, 9, 10, 12, 14, 15, 16, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 5, 6, 7, 11, 13, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 459
cgcagagccc acgcccgact t 21
<210> 460
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 460
gucgggcgug ggcucugcgt t 21
<210> 461
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 8, 9, 11, 12, 16, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 7, 10, 13, 14, 15, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 461
guaagggccg ccggaccgct t 21
<210> 462
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 462
gcgguccggc ggcccuuact t 21
<210> 463
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 6, 7, 9, 11, 12, 13, 16, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 8, 10, 14, 15, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 463
agagcccacg cccgacuagt t 21
<210> 464
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 464
cuagucgggc gugggcucut t 21
<210> 465
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 5, 6, 8, 11, 12, 13, 14, 15, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 7, 9, 10, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 465
guggcuacgg uccucacggt t 21
<210> 466
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 13, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 466
ccgugaggac cguagccact t 21
<210> 467
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 6, 7, 8, 9, 10, 14, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 11, 12, 13, 15, 16, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 467
auaaauccuc agguaguact t 21
<210> 468
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 5, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 468
guacuaccug aggauuuaut t 21
<210> 469
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 6, 8, 9, 11, 14, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 7, 10, 12, 13, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 469
aggcaugccg cgguagguat t 21
<210> 470
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 5, 13
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 470
uaccuaccgc ggcaugccut t 21
<210> 471
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 7, 11, 13, 15, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 5, 6, 8, 9, 10, 12, 14, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 471
gacuagcagg caugccgcgt t 21
<210> 472
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 472
cgcggcaugc cugcuaguct t 21
<210> 473
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 9, 11, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 10, 12, 13, 14, 15, 16, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 473
gaaauaggua cagagacgut t 21
<210> 474
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 10, 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 474
acgucucugu accuauuuct t 21
<210> 475
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 5, 8, 9, 10, 11, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 6, 7, 12, 13, 14, 15
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 475
agucuaaucu cagggccuut t 21
<210> 476
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 476
aaggcccuga gauuagacut t 21
<210> 477
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 8, 9, 11, 14, 15, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 6, 7, 10, 12, 13, 16, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 477
cuuuugacca cagucggaut t 21
<210> 478
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 478
auccgacugu ggucaaaagt t 21
<210> 479
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 4, 5, 7, 10, 11, 15, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 6, 8, 9, 12, 13, 14, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 479
ugaccacagu cggauuaaat t 21
<210> 480
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 480
uuuaauccga cuguggucat t 21
<210> 481
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 4, 5, 7, 10, 11, 15, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 6, 8, 9, 12, 13, 14, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 481
ugaccacagu cggauuaaat t 21
<210> 482
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 482
uuuaauccga cuguggucat t 21
<210> 483
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 4, 6, 10, 13, 14, 15, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 5, 7, 8, 9, 11, 12, 16, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 483
caucaugggc gacucgucat t 21
<210> 484
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 13
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 484
ugacgagucg cccaugaugt t 21
<210> 485
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 5, 8, 9, 10, 11, 16, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 6, 7, 12, 13, 14, 15
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 485
agucuaaucu cagggccuut t 21
<210> 486
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 486
aaggcccuga gauuagacut t 21
<210> 487
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 8, 12, 14, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 4, 5, 6, 7, 9, 10, 11, 13, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 487
accggaacag guaccgagat t 21
<210> 488
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 488
ucucgguacc uguuccggut t 21
<210> 489
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 4, 6, 10, 13, 14, 15, 17, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 5, 7, 8, 9, 11, 12, 16, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 489
caucaugggc gacucgucat t 21
<210> 490
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 490
ugacgagucg cccaugaugt t 21
<210> 491
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 7, 9, 13, 14, 15, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 8, 10, 11, 12, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 491
gagaugcgca gguuccgcat t 21
<210> 492
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 492
ugcggaaccu gcgcaucuct t 21
<210> 493
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 7, 8, 9, 11, 12, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 10, 13, 14, 15, 16, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 493
gaaaggcucg uuaaaaugut t 21
<210> 494
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 494
acauuuuaac gagccuuuct t 21
<210> 495
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 8, 12, 14, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 4, 5, 6, 7, 9, 10, 11, 13, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 495
accggaacag guaccgagat t 21
<210> 496
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 7
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 496
ucucgguacc uguuccggut t 21
<210> 497
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 10, 12, 14, 15, 16, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 6, 7, 8, 9, 11, 13, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 497
ucguuaaaau gugcccagut t 21
<210> 498
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 498
acugggcaca uuuuaacgat t 21
<210> 499
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 5, 6, 11, 12, 13, 14, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 7, 8, 9, 10, 15, 16
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 499
aaucucaggg ccuuaaccut t 21
<210> 500
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 500
agguuaaggc ccugagauut t 21
<210> 501
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 6, 7, 9, 10, 15, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 8, 11, 12, 13, 14, 16, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 501
aaggcucguu aaaaugugct t 21
<210> 502
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 502
gcacauuuua acgagccuut t 21
<210> 503
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 5, 8, 10, 13, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 6, 7, 9, 11, 12, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 503
ggccuagcgc ggcggacggt t 21
<210> 504
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 504
ccguccgccg cgcuaggcct t 21
<210> 505
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 7, 11, 13, 14, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 5, 6, 8, 9, 10, 12, 15, 16, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 505
ccggaacagg uaccgagaut t 21
<210> 506
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 8
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 506
aucucgguac cuguuccggt t 21
<210> 507
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 7, 11, 13, 14, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 5, 6, 8, 9, 10, 12, 15, 16, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 507
ccggaacagg uaccgagaut t 21
<210> 508
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 508
aucucgguac cuguuccggt t 21
<210> 509
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 8, 9, 11, 14, 15, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 6, 7, 10, 12, 13, 16, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 509
cuuuugacca cagucggaut t 21
<210> 510
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 510
auccgacugu ggucaaaagt t 21
<210> 511
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 5, 8, 9, 10, 12, 13, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 6, 7, 11, 14, 15, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 511
ugggcgacuc gucagugcat t 21
<210> 512
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 512
ugcacugacg agucgcccat t 21
<210> 513
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 5, 6, 11, 12, 13, 14, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 7, 8, 9, 10, 15, 16
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 513
aaucucaggg ccuuaaccut t 21
<210> 514
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 514
agguuaaggc ccugagauut t 21
<210> 515
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 5, 8, 9, 10, 12, 13, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 6, 7, 11, 14, 15, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 515
ugggcgacuc gucagugcat t 21
<210> 516
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 516
ugcacugacg agucgcccat t 21
<210> 517
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 5, 6, 8, 11, 12, 16, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 7, 9, 10, 13, 14, 15, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 517
uugaccacag ucggauuaat t 21
<210> 518
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 518
uuaauccgac uguggucaat t 21
<210> 519
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 5, 6, 8, 11, 12, 16, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 7, 9, 10, 13, 14, 15, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 519
uugaccacag ucggauuaat t 21
<210> 520
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 520
uuaauccgac uguggucaat t 21
<210> 521
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 5, 7, 8, 13, 15, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 6, 9, 10, 11, 12, 14, 16
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 521
ggcucguuaa aaugugccct t 21
<210> 522
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 522
gggcacauuu uaacgagcct t 21
<210> 523
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 11, 13, 15, 16, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 4, 7, 8, 9, 10, 12, 14, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 523
cucguuaaaa ugugcccagt t 21
<210> 524
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 8, 13
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 524
cugggcacau uuuaacgagt t 21
<210> 525
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 6, 9, 10, 12, 13, 14, 16, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 5, 7, 8, 11, 15, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 525
cugggcggcu auuuaccagt t 21
<210> 526
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 526
cugguaaaua gccgcccagt t 21
<210> 527
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 7, 9, 13, 14, 15, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 8, 10, 11, 12, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 527
gagaugcgca gguuccgcat t 21
<210> 528
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 528
ugcggaaccu gcgcaucuct t 21
<210> 529
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 5, 7, 8, 13, 15, 17, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 6, 9, 10, 11, 12, 14, 16
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 529
ggcucguuaa aaugugccct t 21
<210> 530
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 6, 11
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 7, 8, 9, 10, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 530
gggcacauuu uaacgagcct t 21
<210> 531
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 6, 7, 9, 10, 15, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 8, 11, 12, 13, 14, 16, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 531
aaggcucguu aaaaugugct t 21
<210> 532
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 4, 9
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 5, 6, 7, 8, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 532
gcacauuuua acgagccuut t 21
<210> 533
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 5, 8, 9, 11, 12, 13, 15, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 6, 7, 10, 14, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 533
ugggcggcua uuuaccaggt t 21
<210> 534
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 534
ccugguaaau agccgcccat t 21
<210> 535
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 4, 7, 9, 12, 16, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 5, 6, 8, 10, 11, 13, 14, 15, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 535
gccuagcgcg gcggacggct t 21
<210> 536
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 536
gccguccgcc gcgcuaggct t 21
<210> 537
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 5, 6, 8, 9, 11, 15, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 7, 10, 12, 13, 14, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 537
aaguucauca ugggcgacut t 21
<210> 538
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 538
agucgcccau gaugaacuut t 21
<210> 539
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 11, 13, 15, 16, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 4, 7, 8, 9, 10, 12, 14, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 539
cucguuaaaa ugugcccagt t 21
<210> 540
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 540
cugggcacau uuuaacgagt t 21
<210> 541
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 6, 8, 11, 15, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 4, 5, 7, 9, 10, 12, 13, 14, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 541
ccuagcgcgg cggacggcct t 21
<210> 542
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 542
ggccguccgc cgcgcuaggt t 21
<210> 543
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 3, 4, 5, 8, 10, 13, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 6, 7, 9, 11, 12, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 543
ggccuagcgc ggcggacggt t 21
<210> 544
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 544
ccguccgccg cgcuaggcct t 21
<210> 545
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 6, 8, 11, 15, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 4, 5, 7, 9, 10, 12, 13, 14, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 545
ccuagcgcgg cggacggcct t 21
<210> 546
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 546
ggccguccgc cgcgcuaggt t 21
<210> 547
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 3, 4, 7, 9, 12, 16, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 5, 6, 8, 10, 11, 13, 14, 15, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 547
gccuagcgcg gcggacggct t 21
<210> 548
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 548
gccguccgcc gcgcuaggct t 21
<210> 549
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 5, 6, 8, 9, 11, 15, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 7, 10, 12, 13, 14, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 549
aaguucauca ugggcgacut t 21
<210> 550
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 8
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 7, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 550
agucgcccau gaugaacuut t 21
<210> 551
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 7, 9, 12, 13, 14, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 8, 10, 11, 15, 16, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 551
gagcgacgcg gccuagcgct t 21
<210> 552
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 552
gcgcuaggcc gcgucgcuct t 21
<210> 553
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 7, 8, 9, 11, 12, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 10, 13, 14, 15, 16, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 553
gaaaggcucg uuaaaaugut t 21
<210> 554
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 2, 7
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 3, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 554
acauuuuaac gagccuuuct t 21
<210> 555
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 4, 5, 10, 12, 14, 15, 16, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 6, 7, 8, 9, 11, 13, 17, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 555
ucguuaaaau gugcccagut t 21
<210> 556
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 7, 9, 14
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 8, 10, 11, 12, 13, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 556
acugggcaca uuuuaacgat t 21
<210> 557
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 5, 8, 9, 11, 12, 13, 15, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 3, 4, 6, 7, 10, 14, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 557
ugggcggcua uuuaccaggt t 21
<210> 558
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 10, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 7, 8, 9, 11, 12, 13, 14, 15, 16, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 558
ccugguaaau agccgcccat t 21
<210> 559
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 6, 9, 10, 12, 13, 14, 16, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 3, 4, 5, 7, 8, 11, 15, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 559
cugggcggcu auuuaccagt t 21
<210> 560
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 5, 9, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 6, 7, 8, 10, 11, 12, 13, 14, 15, 16, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 560
cugguaaaua gccgcccagt t 21
<210> 561
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 7, 8, 10, 11, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 9, 12, 13, 14, 15, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 561
aaaggcucgu uaaaaugugt t 21
<210> 562
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 562
cacauuuuaa cgagccuuut t 21
<210> 563
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 6, 7, 12, 14, 15, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 8, 9, 10, 11, 13, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 563
agguaccgag augucagcct t 21
<210> 564
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 564
ggcugacauc ucgguaccut t 21
<210> 565
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 6, 7, 12, 14, 15, 18, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 8, 9, 10, 11, 13, 16, 17
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 565
agguaccgag augucagcct t 21
<210> 566
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 7, 15
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 566
ggcugacauc ucgguaccut t 21
<210> 567
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 4, 7, 9, 12, 13, 14, 17, 19
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 6, 8, 10, 11, 15, 16, 18
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 567
gagcgacgcg gccuagcgct t 21
<210> 568
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1..19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 568
gcgcuaggcc gcgucgcuct t 21
<210> 569
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 6, 7, 8, 10, 11, 16, 18
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 9, 12, 13, 14, 15, 17, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 569
aaaggcucgu uaaaaugugt t 21
<210> 570
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 3, 8
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 2, 4, 5, 6, 7, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 570
cacauuuuaa cgagccuuut t 21
<210> 571
<211> 38
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human PTP1B
<400> 571
gcgtcgcgct cttagccttt ttctcttgga aagaaagt 38
<210> 572
<211> 39
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human PTP1B
<400> 572
cgtctgccgc tctaggcctt tttctcttgg aaagaaagt 39
<210> 573
<211> 37
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human PTP1B
<400> 573
gcggctgctg cgcctctttt tctcttggaa agaaagt 37
<210> 574
<211> 39
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human PTP1B
<400> 574
tctccatgac gggccaggtt tttctcttgg aaagaaagt 39
<210> 575
<211> 42
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human PTP1B
<400> 575
tctgctcgaa ctccttttcc atttttctct tggaaagaaa gt 42
<210> 576
<211> 39
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human PTP1B
<400> 576
tcctggtaaa tggccgcctt tttctcttgg aaagaaagt 39
<210> 577
<211> 45
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human PTP1B
<400> 577
atttcggttt ttgttcttag gaagtttttc tcttggaaag aaagt 45
<210> 578
<211> 42
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human PTP1B
<400> 578
ccccgacccc tgcagctatt tttaggcata ggacccgtgt ct 42
<210> 579
<211> 41
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human PTP1B
<400> 579
ccgaggcccg ctgcaatttt ttaggcatag gacccgtgtc t 41
<210> 580
<211> 42
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human PTP1B
<400> 580
cttctcggcc cactgcgctt tttaggcata ggacccgtgt ct 42
<210> 581
<211> 43
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human PTP1B
<400> 581
cagctcccgg acttgtcgat ttttaggcat aggacccgtg tct 43
<210> 582
<211> 46
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human PTP1B
<400> 582
gtcactggct tcatgtcgga tatttttagg cataggaccc gtgtct 46
<210> 583
<211> 47
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human PTP1B
<400> 583
aagggactga cgtctctgta ccttttttag gcataggacc cgtgtct 47
<210> 584
<211> 50
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human PTP1B
<400> 584
gatgtagttt aatccgacta tggtcatttt taggcatagg acccgtgtct 50
<210> 585
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human PTP1B
<400> 585
cttggccact ctacatggga a 21
<210> 586
<211> 41
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human GAPDH
<400> 586
gaatttgcca tgggtggaat tttttctctt ggaaagaaag t 41
<210> 587
<211> 41
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human GAPDH
<400> 587
ggagggatct cgctcctgga tttttctctt ggaaagaaag t 41
<210> 588
<211> 40
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human GAPDH
<400> 588
ccccagcctt ctccatggtt ttttctcttg gaaagaaagt 40
<210> 589
<211> 40
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human GAPDH
<400> 589
gctcccccct gcaaatgagt ttttctcttg gaaagaaagt 40
<210> 590
<211> 42
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human GAPDH
<400> 590
agccttgacg gtgccatgtt tttaggcata ggacccgtgt ct 42
<210> 591
<211> 45
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human GAPDH
<400> 591
gatgacaagc ttcccgttct ctttttaggc ataggacccg tgtct 45
<210> 592
<211> 46
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human GAPDH
<400> 592
agatggtgat gggatttcca tttttttagg cataggaccc gtgtct 46
<210> 593
<211> 44
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human GAPDH
<400> 593
gcatcgcccc acttgatttt tttttaggca taggacccgt gtct 44
<210> 594
<211> 43
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human GAPDH
<400> 594
cacgacgtac tcagcgccat ttttaggcat aggacccgtg tct 43
<210> 595
<211> 46
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human GAPDH
<400> 595
ggcagagatg atgacccttt tgtttttagg cataggaccc gtgtct 46
<210> 596
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for human GAPDH
<400> 596
ggtgaagacg ccagtggact c 21
<210> 597
<211> 45
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat PTP1B
<400> 597
ggtttttgtt cttaggaagc ttcgtttttc tcttggaaag aaagt 45
<210> 598
<211> 44
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat PTP1B
<400> 598
tcattatctt cctggtgcaa ttttttttct cttggaaaga aagt 44
<210> 599
<211> 42
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat PTP1B
<400> 599
gctcctctgg gcttcttcca ttttttctct tggaaagaaa gt 42
<210> 600
<211> 42
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat PTP1B
<400> 600
agggccctgg gtgagaatat atttttctct tggaaagaaa gt 42
<210> 601
<211> 47
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat PTP1B
<400> 601
cgaactcctt ctccatctcc atgtttttag gcataggacc cgtgtct 47
<210> 602
<211> 44
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat PTP1B
<400> 602
cccagccttg tcgatctcct tttttaggca taggacccgt gtct 44
<210> 603
<211> 44
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat PTP1B
<400> 603
tggtaaatag ccgcccagtt tttttaggca taggacccgt gtct 44
<210> 604
<211> 45
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat PTP1B
<400> 604
ctggcttcat gtcgaatgtc ctttttaggc ataggacccg tgtct 45
<210> 605
<211> 47
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat PTP1B
<400> 605
gctgacatct cggtacctgt tcctttttag gcataggacc cgtgtct 47
<210> 606
<211> 45
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat PTP1B
<400> 606
aatccgactg tggtcaaaag gtttttaggc ataggacccg tgtct 45
<210> 607
<211> 49
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat PTP1B
<400> 607
ttttatcaag ctggcattga tatagttttt aggcatagga cccgtgtct 49
<210> 608
<211> 20
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat PTP1B
<400> 608
cgactttgca tgggaagtcg 20
<210> 609
<211> 41
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat GAPDH
<400> 609
ccagcttccc attctcagcc tttttctctt ggaaagaaag t 41
<210> 610
<211> 41
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat GAPDH
<400> 610
tctcgctcct ggaagatggt tttttctctt ggaaagaaag t 41
<210> 611
<211> 41
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat GAPDH
<400> 611
cccatttgat gttagcggga tttttctctt ggaaagaaag t 41
<210> 612
<211> 42
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat GAPDH
<400> 612
cggagatgat gacccttttg gtttttctct tggaaagaaa gt 42
<210> 613
<211> 44
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat GAPDH
<400> 613
gatgggtttc ccgttgatga tttttaggca taggacccgt gtct 44
<210> 614
<211> 47
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat GAPDH
<400> 614
gacatactca gcaccagcat cactttttag gcataggacc cgtgtct 47
<210> 615
<211> 44
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat GAPDH
<400> 615
cccagccttc tccatggtgg tttttaggca taggacccgt gtct 44
<210> 616
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat GAPDH
<400> 616
ttgactgtgc cgttgaactt g 21
<210> 617
<211> 22
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat GAPDH
<400> 617
tgaagacgcc agtagactcc ac 22
<210> 618
<211> 19
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat GAPDH
<400> 618
ccccaccctt caggtgagc 19
<210> 619
<211> 17
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: bDNA probes for rat GAPDH
<400> 619
ggcatcagcg gaagggg 17
<210> 620
<211> 3318
<212> DNA
<213> Homo sapiens
<220>
<223> cDNA sequence of human PTP1B
<300>
<308> NM_002827.2
<309> 2008-12-21
<400> 620
gtgatgcgtagttccggctgccggttgacatgaagaagcagcagcggctagggcggcggt 60
agctgcaggggtcggggattgcagcgggcctcggggctaagagcgcgacgcggcctagag 120
cggcagacggcgcagtgggccgagaaggaggcgcagcagccgccctggcccgtcatggag 180
atggaaaaggagttcgagcagatcgacaagtccgggagctgggcggccatttaccaggat 240
atccgacatgaagccagtgacttcccatgtagagtggccaagcttcctaagaacaaaaac 300
cgaaataggtacagagacgtcagtccctttgaccatagtcggattaaactacatcaagaa 360
gataatgactatatcaacgctagtttgataaaaatggaagaagcccaaaggagttacatt 420
cttacccagggccctttgcctaacacatgcggtcacttttgggagatggtgtgggagcag 480
aaaagcaggggtgtcgtcatgctcaacagagtgatggagaaaggttcgttaaaatgcgca 540
caatactggccacaaaaagaagaaaaagagatgatctttgaagacacaaatttgaaatta 600
acattgatctctgaagatatcaagtcatattatacagtgcgacagctagaattggaaaac 660
cttacaacccaagaaactcgagagatcttacatttccactataccacatggcctgacttt 720
ggagtccctgaatcaccagcctcattcttgaactttcttttcaaagtccgagagtcaggg 780
tcactcagcccggagcacgggcccgttgtggtgcactgcagtgcaggcatcggcaggtct 840
ggaaccttctgtctggctgatacctgcctcttgctgatggacaagaggaaagacccttct 900
tccgttgatatcaagaaagtgctgttagaaatgaggaagtttcggatggggctgatccag 960
acagccgaccagctgcgcttctcctacctggctgtgatcgaaggtgccaaattcatcatg 1020
ggggactcttccgtgcaggatcagtggaaggagctttcccacgaggacctggagccccca 1080
cccgagcatatccccccacctccccggccacccaaacgaatcctggagccacacaatggg 1140
aaatgcagggagttcttcccaaatcaccagtgggtgaaggaagagacccaggaggataaa 1200
gactgccccatcaaggaagaaaaaggaagccccttaaatgccgcaccctacggcatcgaa 1260
agcatgagtcaagacactgaagttagaagtcgggtcgtggggggaagtcttcgaggtgcc 1320
caggctgcctccccagccaaaggggagccgtcactgcccgagaaggacgaggaccatgca 1380
ctgagttactggaagcccttcctggtcaacatgtgcgtggctacggtcctcacggccggc 1440
gcttacctctgctacaggttcctgttcaacagcaacacatagcctgaccctcctccactc 1500
cacctccacccactgtccgcctctgcccgcagagcccacgcccgactagcaggcatgccg 1560
cggtaggtaagggccgccggaccgcgtagagagccgggccccggacggacgttggttctg 1620
cactaaaacccatcttccccggatgtgtgtctcacccctcatccttttactttttgcccc 1680
ttccactttgagtaccaaatccacaagccattttttgaggagagtgaaagagagtaccat 1740
gctggcggcgcagagggaaggggcctacacccgtcttggggctcgccccacccagggctc 1800
cctcctggagcatcccaggcgggcggcacgccaacagccccccccttgaatctgcaggga 1860
gcaactctccactccatatttatttaaacaattttttccccaaaggcatccatagtgcac 1920
tagcattttcttgaaccaataatgtattaaaattttttgatgtcagccttgcatcaaggg 1980
ctttatcaaaaagtacaataataaatcctcaggtagtactgggaatggaaggctttgcca 2040
tgggcctgctgcgtcagaccagtactgggaaggaggacggttgtaagcagttgttattta 2100
gtgatattgtgggtaacgtgagaagatagaacaatgctataatatataatgaacacgtgg 2160
gtatttaataagaaacatgatgtgagattactttgtcccgcttattctcctccctgttat 2220
ctgctagatctagttctcaatcactgctcccccgtgtgtattagaatgcatgtaaggtct 2280
tcttgtgtcctgatgaaaaatatgtgcttgaaatgagaaactttgatctctgcttactaa 2340
tgtgccccatgtccaagtccaacctgcctgtgcatgacctgatcattacatggctgtggt 2400
tcctaagcctgttgctgaagtcattgtcgctcagcaatagggtgcagttttccaggaata 2460
ggcatttgcctaattcctggcatgacactctagtgacttcctggtgaggcccagcctgtc 2520
ctggtacagcagggtcttgctgtaactcagacattccaagggtatgggaagccatattca 2580
cacctcacgctctggacatgatttagggaagcagggacaccccccgccccccacctttgg 2640
gatcagcctccgccattccaagtcaacactcttcttgagcagaccgtgatttggaagaga 2700
ggcacctgctggaaaccacacttcttgaaacagcctgggtgacggtcctttaggcagcct 2760
gccgccgtctctgtcccggttcaccttgccgagagaggcgcgtctgccccaccctcaaac 2820
cctgtggggcctgatggtgctcacgactcttcctgcaaagggaactgaagacctccacat 2880
taagtggctttttaacatgaaaaacacggcagctgtagctcccgagctactctcttgcca 2940
gcattttcacattttgcctttctcgtggtagaagccagtacagagaaattctgtggtggg 3000
aacattcgaggtgtcaccctgcagagctatggtgaggtgtggataaggcttaggtgccag 3060
gctgtaagcattctgagctgggcttgttgtttttaagtcctgtatatgtatgtagtagtt 3120
tgggtgtgtatatatagtagcatttcaaaatggacgtactggtttaacctcctatccttg 3180
gagagcagctggctctccaccttgttacacattatgttagagaggtagcgagctgctctg 3240
ctatatgccttaagccaatatttactcatcaggtcattattttttacaatggccatggaa 3300
taaaccatttttacaaaa 3318
<210> 621
<211> 5092
<212> DNA
<213> Macaca mulatta
<220>
<223> Consensus cDNA sequence of rhesus monkey PTP1B
<400> 621
aggcgcggtgcgtagtccgccggttgacatgaagaagccgcggcggctagggcggcggta 60
gcggcaggagtcggtgcttgctgcggacctcagggctaagagcgcgacgcggcctagagc 120
ggcggactgcgcagtgggccgagaaggaggcgcagcagccgccctggcccgtcatggaga 180
tggaaaaggagttcgagcagatcgacaagtccgggagctgggcggccatttaccaggata 240
tccgacacgaagccagtgacttcccatgtagagtggccaagcttcctaagaacaaaaacc 300
gaaataggtacagagacgtcagtccctttgaccatagtcggattaaactacatcaagaag 360
ataacgactatatcaacgctagtttgataaaaatggaagaagcccaaaggagttacatcc 420
ttacccagggccctttgcctaacacatgcggtcacttttgggagatggtgtgggaacaga 480
aaagcaggggtgtcgtcatgctcaacagagtgatggagaaaggttcgttaaaatgcgcac 540
agtactggccacaaaaagaagaaaaagaaatgatctttgaagacacaaacttgaaattaa 600
cattgatctctgaagatatcaaatcatattatacagtgcgacagctagaattggaaaacc 660
ttacaacccaagaaactcgagagatcttacatttccactataccacatggcctgactttg 720
gagtccccgaatcgccagcctcattcttgaactttcttttcaaagtccgagagtcagggt 780
cactcagcccggagcatgggcccgtcgtggtgcactgcagtgcgggtatcggcaggtctg 840
ggaccttctgtctggctgatacctgcctcttgctgatggacaagaggaaagacccttctt 900
ccgttgatatcaagaaagtgctattagaaatgaggaagtttcggatggggctgatccaga 960
cagcagaccagctgcgcttctcctacttggctgtgatcgaaggtgccaaattcatcatgg 1020
gggactcctctgtgcaggatcagtggaaggagctttcccacgaggacctggagcccccac 1080
ccgagcacgtccccccacctccccggccacccaaacgaatcctggagccacacaatggga 1140
aatgcagggagttcttcccaaatcaccagtgggtgaaggatgagacccaggaggataaag 1200
actgccccatcaaggaagaaacaggaagccccttaaatgccgcaccctacagcatggaaa 1260
gcatgagtcaagacactgaagttagaagtcgggtcgtggggggaagtcttcgaggtgccc 1320
aggctgcctccctagccaaaggggagccgtcaccgcctaagaaggaggaggaccatgcac 1380
tgagtcactggaagcccttcctggtcaacatgtgtgtggctacggtcctcacggccggcg 1440
cgtacctctgctacagggtatgtttccactgacagacgcgctggcgagatgctcgtgtgc 1500
agagagcactggccgctagcccgatggtaggattcagttctgtggcgcatctgagccagt 1560
ctcagaagaaacagatcaaaggtttttaaagtctggaactgtggaagggctaacgagaat 1620
taaggatcgatgcactggggttttaaggagccctctggtcccaagaatataagagtctaa 1680
tctcagggccttaacctattcaggagtaagtagagaaaatgccaaatacgtctgtctctc 1740
tctctctctcttttttttattcctttgtttttggaaaaaaatagaattacaacacattgt 1800
tgtttttaacctttataaaaagcagctttttgttatttctggaaaaaaaacaaactaggc 1860
acttaatgaaactttctcgtacccttaggtgatgtaattagctatataatttatatttga 1920
tttcccagggaaggaatcccaaacttttacgaatgtaaactcccttggagaagagggtta 1980
ggacgctcttgcgctcaagcccccctcagctgtgtgcacactgagccaggacagggtctt 2040
tgagctttcccactgtaagaacagcaacacaaggccgtctagagaaacagaacctgcctc 2100
tgcttctgctcagggtgtccgttgtcctttctccattgctccctcctgtgacagccatct 2160
tgctcatgtaccagccctcatcaccccattcccataaaagagtgtcctcgaggcctctcc 2220
ctgggggtcagaggtcaccacagggtggccctcagcatgtcagccctctgttaattcaga 2280
ggagtgggctccacctcattgggagaagtgccatttcagcagaaatccacacgttagacg 2340
tgtgttgctgttaagtaaggggaagagaggactagcctcagagctctggccatggaaatg 2400
acctcctgagactttttcatcgttttaaatattttacctcttttcaggtggccatctgag 2460
tacatcagatggttttgcaaaatgcaaacaattttttccttgggggtgatttttggggag 2520
aaggggctactgtaaaaaataaaaccaaaaccccctttgctccctcggaggttgaagttg 2580
ccggggggtgtggccggggtcgtgcatgaggcgacagctctgcgggtgcgggtctgggct 2640
catctgaactgtttggtttcattccagttcctgttcaacagcaacacatagcctgaccct 2700
cctccactccacctccacccactgtccgcctctgcccgcagagcccacgcccgactagca 2760
ggcatgccgcggtaggtaagggccgccggaccgcgtagagagccgggccccggacggacg 2820
ttggttctgcactaaaacccatcttccccggatgtgtgtctcacccctcatccttttact 2880
ttttgccccttccactttgagtaccaaatccacaagccattttttgaggagagtgaaaga 2940
gtaccatgctggcggcgcagagggagggggcctgcacctgtctggaggctcgccccaccc 3000
agggctccctcctggagcatcccaggcaggtggtacaccaacagcccccttgactcgcag 3060
ggagcaactctccactccatatttatttaaacaatttttccccaaaggtgtccatagtgc 3120
actagcattttcttgaaccaataatgtattaaaaatttttgatgtcagccttgcatcaag 3180
ggctttatcaaaaagtacaataataaatcctcaggtagtactgggaatggaagactttgc 3240
catgggcctgctgcgtcagaccagtactgggaaggaggacggttgtaaggcagtcgttat 3300
ttagtgatattgtgggtaacgtgagaagatagaacaatgctataatatataatgaacacg 3360
tgggtatttaataagaaacatgatgtgagattactttgtcccgcttattctcctccctgt 3420
tatctgctagatctagttctcaatcactgctcccccgtgtgtactagaatgcatgtaagg 3480
tcttcttgtgtcctgatgaaaaatatgtgcttgaaatgagaaactttgatctctgcttac 3540
taatgtgccccatgtccaagtccaacctgcctgtgcatgacctgatcattacatggctgt 3600
ggttcctaagcctgttgctgaagtcgttgtcactcagcaatagggtgcagttttccagga 3660
ataggcatttgcctcattcctggcctgacactctagtgacttcctggtgagacccggcct 3720
gtcctggtgcagcagggtctcgctgtaactcagacattccaagggtatgggaagccatat 3780
tcacacctcacgctctggacattatttaggcaagcagggacaccccccgccccccacctt 3840
tgggatcagcctccgccattccaagtcagcactcttcttgagcagactgtgatttggaag 3900
agaggcaactgctggaaaccacacttcttgaaacagcctgggtgacggtcctttaggcag 3960
cctgccgccgtctccgtctgggttcaccttgccgagagaggcgcgtctgccccaccctcg 4020
aaccctgtggggcctgatggtgctcacgactcttcctgcaaagggaactgaagacctcca 4080
cattaagtggctttttaacaagaaaaacacggcagctgtagctcccgagctactctcttg 4140
ccagcattttcacattttgcctttctcgtggtagaagccagtacagagaaattctgtcgt 4200
gggaacattcgaggcatcaccccatagagctgtggtgaggtgtggataaggcttaggtgc 4260
caggctgtaagcattctgagctgggcttgttgtttttaagtcctgtatatgtatgtagta 4320
gtttgggtgtgtatatatagtagcatttcaaaatggacgtactggtttaacctcctatcc 4380
tcggagagcagctggctctccaccttgttacacgtatgttagagaggtagcgagctgctc 4440
tgctatatgccttaagccaatatttactcatcaggtcattattttttacaatggccatgg 4500
aataaaccatttttacaaaaataaaaacaaaaaaagcaaggtgttttggtataatacctt 4560
ttcaggtgtgtgtggatacgtggctgcatgaccgggtgggtgggggggcgtgtctcaggg 4620
tcttctgtgacctcacagaactgtcagactgtacagttttccaacttgccatattcatga 4680
tgggtttgcgttttagctgcaacaataaagttttttttctaaagaacgtgaatttggggt 4740
gcttcccattttttttttctttgcttaattgagctaaaccaggatgagcaactcctgttt 4800
ctttccatccctgctgatgtgaaacagatgttgtcaatcagctggggttagagttttcca 4860
cttctaagaattaacctcagcatccccgcgttgccagcgccctcaggctggagcgctttc 4920
cttgagggtgagcttactgaacaccttaggcctcagcccacttccttcccaaacccacgc 4980
tttccatgtagagggcccttggataacttaacaaacttatcagtgttgtttgaagaacag 5040
tgttttgagttgtaatctcaaaaccatgtcccttacccaattacctgtaaga 5092
<210> 622
<211> 1840
<212> DNA
<213> Macaca fascicularis
<220>
<223> cDNA sequence of cynomolgous monkey PTP1B (partial)
<400> 622
gatatccgacacgaagccagtgacttcccatgtagagtggccaagcttcctaagaacaaa 60
aaccgaaataggtacagagacgtcagtccctataaaaatggaagaagcccaaaggagtta 120
catccttacccagggccctttgcctaacacatgcggtcacttttgggagatggtgtggga 180
acagaaaagcaggggtgtcgtcatgctcaacagagtgatggagaaaggttcgttaaaatg 240
cgcacagtactggccacaaaaagaagaaaaagaaatgatctttgaagacacaaacttgaa 300
attaacattgatctctgaagatatcaaatcatattatacagtgcgacagctagaattgga 360
aaaccttacaacccaagaaactcgagagatcttacatttccactataccacatggcctga 420
ctttggagtccccgaatcgccagcctcattcctgaactttcttttcaaagtccgagagtc 480
agggtcactcagcccggagcatgggcccgtcgtggtgcactgcagtgcgggtatcgatta 540
gaaatgaggaagtttcggatggggctgatccagacagcagaccagctgcgcttctcctac 600
ttggctgtgatcgaaggtgccaaattcatcatgggggactcctctgtgcaggatcagtgg 660
aaggagcttttcccacgaggacctggacgccccccacatgagtcaagacactgaagttag 720
aagtcgggtcgtggggggaagtcttcgaggtgcccaggctgcctccctagccaaagggga 780
gccgtcaccgcctaagaaggaggaggaccatgcactgagtcactggaagcccttcctggt 840
caacatgtgtgtggctacggtcctcacggccggcgcgtacctctgctacagggtatgttt 900
ccacgatatccgacacgaagccagtgacttcccatgtagagtggccaagcttcctaagaa 960
caaaaaccgaaataggtacagagacgtcagtccctaagaagataacgactatatcaacgc 1020
tagtttgataaaaatggaagaagcccaaaggagttacatccttacccagggccctttgcc 1080
taacacatgcggtcacttttgggagatggtgtgggaacagaaaagcaggggtgtcgtcat 1140
gctcaacagagtgatggagaaaggttcgttaaaatgcgcacagtactggccacaaaaaga 1200
agaaaaagaaatgatctttgaagacacaaacttgaaattaacattgatctctgaagatat 1260
caaatcatattatacagtgcgacagctagaattggaaaaccttacaacccaagaaactcg 1320
agagatcttacatttccactataccacatggcctgactttggagtccccgaatcgccagc 1380
ctcattcctgaactttcttttcaaagtccgagagtcagggtcactcagcccggagcatgg 1440
gcccgtcgtggtgcactgcagtgcgggtatcgattagaaatgaggaagtttcggatgggg 1500
ctgatccagacagcagaccagctgcgcttctcctacttggctgtgatcgaaggtgccaaa 1560
ttcatcatgggggactcctctgtgcaggatcagtggaaggagcttttcccacgaggacct 1620
ggacgccccccacatgagtcaagacactgaagttagaagtcgggtcgtggggggaagtct 1680
tcgaggtgcccaggctgcctccctagccaaaggggagccgtcaccgcctaagaaggagga 1740
ggaccatgcactgagtcactggaagcccttcctggtcaacatgtgtgtggctacggtcct 1800
cacggccggcgcgtacctctgctacagggtatgtttccac 1840
<210> 623
<211> 4213
<212> DNA
<213> Mus musculus
<220>
<223> cDNA sequence of mouse PTP1B
<300>
<308> NM_011201.3
<309> 2008-12-21
<400> 623
gcggtgaggcgggggtgcgtacttccggctgccggcttgacatgcagaagccgctgctgg 60
ggagcttggggctgcggtggtggcgggtgacgggcttcgggacgcggagcgacgcggcct 120
agcgcggcggacggccgtgggaactcgggcagccgacccgtcccgccatggagatggaga 180
aggagttcgaggagatcgacaaggctgggaactgggcggctatttaccaggacattcgac 240
atgaagccagcgacttcccatgcaaagtcgcgaagcttcctaagaacaaaaaccggaaca 300
ggtaccgagatgtcagcccttttgaccacagtcggattaaattgcaccaggaagataatg 360
actatatcaatgccagcttgataaaaatggaagaagcccagaggagctatattctcaccc 420
agggccctttaccaaacacatgtgggcacttctgggagatggtgtgggagcagaagagca 480
ggggcgtggtcatgctcaaccgcatcatggagaaaggctcgttaaaatgtgcccagtatt 540
ggccacagcaagaagaaaaggagatggtctttgatgacacaggtttgaagttgacactaa 600
tctctgaagatgtcaagtcatattacacagtacgacagttggagttggaaaacctgacta 660
ccaaggagactcgagagatcctgcatttccactacaccacatggcctgactttggagtcc 720
ccgagtcaccggcttctttcctcaatttccttttcaaagtccgagagtcaggctcactca 780
gcctggagcatggccccattgtggtccactgcagcgccggcatcgggaggtcagggacct 840
tctgtctggctgacacctgcctcttactgatggacaagaggaaagacccatcttccgtgg 900
acatcaagaaagtactgctggagatgcgcaggttccgcatggggctcatccagactgccg 960
accagctgcgcttctcctacctggctgtcatcgagggcgccaagttcatcatgggcgact 1020
cgtcagtgcaggatcagtggaaggagctctcccgggaggatctagaccttccacccgagc 1080
acgtgcccccacctccccggccacccaaacgcacactggagcctcacaacgggaagtgca 1140
aggagctcttctccagccaccagtgggtgagcgaggagacctgtggggatgaagacagcc 1200
tggccagagaggaaggcagagcccagtcaagtgccatgcacagcgtgagcagcatgagtc 1260
cagacactgaagttaggagacggatggtgggtggaggtcttcaaagtgctcaggcgtctg 1320
tccccaccgaggaagagctgtcctccactgaggaggaacacaaggcacattggccaagtc 1380
actggaagcccttcctggtcaatgtgtgcatggccacgctcctggccaccggcgcgtact 1440
tgtgctaccgggtgtgttttcactgacagactgggaggcactgccactgcccagcttagg 1500
atgcggtctgcggcgtctgacctggtgtagagggaacaacaactcgcaagcctgctctgg 1560
aactggaagggccagccccaggagggtattagtgcactgggctttgaaggagcccctggt 1620
cccacgaacagagtctaatctcagggccttaacctgttcaggagaagtagaggaaatgcc 1680
aaatactcttcttgctctcacctcactcctcccctttctctgattcatttgtttttggaa 1740
aaaaaaaaaaaaagaattacaacacattgttgtttttaacatttataaaggcaggttttt 1800
gttatttctggagaaaaaaaaaagatgctaggcactggtgagcttctctcgtgccctttg 1860
gcatgtgatcagattcatgatttctattgatttccagtggaaggatcccgctgggcagga 1920
ctgtaaagttcctgctggcgtgggcagcccccactgtgcacccggagcttgcaggtgccc 1980
tggagccccatcctgttgtgaggagggcagccacatgggctgcctctgaacagcaagccc 2040
ggctctgcttcgctcaggtgtcccccccgcggttccatccttctcggtctcctccctctt 2100
tgtgagtgccatcttgcaaatggcccagcccttgccaccctatccctgtaaatccctgag 2160
ggccttgcctcggggtcagaggtggctgccagcctaagtgctggggtttccatttcattt 2220
ggaaagtcattactactctgtgtggaagccactctactgaggtgcaagcaagactggtga 2280
aggagaaaggagccctggtgtcatggaagcccctcggcaggacctgtacccccctctgaa 2340
acactgttccagtgaacgctctcgaaggcatcagatagaggatttttgcagactgcaaag 2400
acttcctgagtggtgattttcagagtctggactcaaaaaacaccatggagcgcttaccgc 2460
gctgccttccatgctggtatggctgaggccgtggggaggccggcgctgtggctccatcca 2520
gtgcggatgcctttgtgcaatcgctgcccagcagcagagcctgcatgccagcctctgcgc 2580
tgcaggccacaatggccatggcggcccaaagagtgagctgcgatggaggcttatcacatg 2640
gagctgccttttcaggctatgcatctcccttcctgctttaccctctgcttccttcccgag 2700
tagcaagcccaccatcatgaggagtgcaagggaggggcaggcattagagcggggtgcggc 2760
tttcgaggtgaggccacctccgaggctgccctcctcttatttattcactttgcccggagg 2820
tggcccccgcgtgagcatgccctgaaactgaccgtgtctgcctgcacccatgaccgtcag 2880
gtcagtcctggggttgaggcatgagtgcaggactgacaggagccacagtatggccagtgc 2940
tggggccaggagcaggagcaggagggtgtgaagagcagccttcaggagcataggacagtg 3000
gtaatgcgcagagcccctgtggctgctcctgcgacgtgcagggtggaggctgggctttct 3060
gcccacgaccttctagatcccattcccggcctctgctttccctgtgagttagaagataca 3120
cagttctgtgctggtggcaccttgtgcttgacatgggaaacttagaagcctgctcactga 3180
caggcacctcccgtgctctgaccggaagtggccgtcgctgagtaaccaccgccactcagt 3240
gagagcagtgctgtccagtcaggtgccacctgactcctggcaggacacttcaggcttccg 3300
tcctgccactccacgccacgccacgccatgccccgctggatctcagacattccacactca 3360
cacctcattccctggacacttaggcaagcagggttcttccacctcttggatcagcccctc 3420
cattccaagttcatgctgtcctggagcaggccaggactggaagcaaggcagcttgtgaga 3480
agcaacctcctgggaacagtatggatgacagccttagagtcggcctgctgtcattgctgg 3540
ccccagggccccttacccagaagtgtgcagctctccggcctgggagagacggatgacggt 3600
gctcatgactcttctgggaggggaagaaccgagccttcacattgggtggctttttaaaat 3660
aagcgaaggcagctggaactccgctgcctcgtgccagcatttcacattttgccttttacc 3720
cagagaagccagcacagagccactggggaacagtgatagccttgcctgcagagcctgagg 3780
aggtggcacagccagggtccaggctgggagcagttagcagtgggctgtgtctggagtagt 3840
gtggtgtgtacagtagccttagaggtggctctcccggagaacaggcgctctctgccctgt 3900
cgcccctgttggaggtagcaagctgctgtatgccttaagtcaacatttactcagcagggc 3960
tctgtctctctctctctttgtctttctctctctctctctgtctctctctctctctaaatg 4020
gccatagaataaaccatttttacaaaaataaaaaccaacaaaaaactgtgccctggaaga 4080
gtacctttgcaggagtgtggggtgtctcagggtcttctgtgacctcacagagctgtctga 4140
ctgcaccatttccaacttgctgtctcactaatgggtctgcattagttgcaacaataaatg 4200
tttttaaagaaca 4213
<210> 624
<211> 1744
<212> DNA
<213> Rattus norvegicus
<220>
<223> cDNA sequence of rat PTP1B
<300>
<308> NM_012637.2
<309> 2008-12-21
<400> 624
cgggcctcgagacgcggagcgacgcggcctagcgcggcggacggccgagggaactcgggc 60
agtcgtcccgtcccgccatggaaatggagaaggaattcgagcagatcgataaggctggga 120
actgggcggctatttaccaggatattcgacatgaagccagtgacttcccatgcagaatag 180
cgaaacttcctaagaacaaaaaccggaacaggtaccgagatgtcagcccttttgaccaca 240
gtcggattaaattgcatcaggaagataatgactatatcaatgccagcttgataaaaatgg 300
aggaagcccagaggagctatatcctcacccagggccctttaccaaacacgtgcgggcact 360
tctgggagatggtgtgggagcagaagagcaggggcgtggtcatgctcaaccgcatcatgg 420
agaaaggctcgttaaaatgtgcccagtattggccacagaaagaagaaaaagagatggtct 480
tcgatgacaccaatttgaagctgacactgatctctgaagatgtcaagtcatattacacag 540
tacggcagttggagttggagaacctggctacccaggaggctcgagagatcctgcatttcc 600
actacaccacctggcctgactttggagtccctgagtcacctgcctctttcctcaatttcc 660
tattcaaagtccgagagtcaggctcactcagcccagagcacggccccattgtggtccact 720
gcagtgctggcattggcaggtcagggaccttctgcctggctgacacctgcctcttactga 780
tggacaagaggaaagacccgtcctctgtggacatcaagaaagtgctgttggagatgcgca 840
ggttccgcatggggctcatccagacggccgaccaactgcgcttctcctacctggctgtga 900
tcgagggtgcaaagttcatcatgggcgactcgtcagtgcaggatcagtggaaggagcttt 960
cccatgaagacctggagcctccccctgagcacgtgcccccacctccccggccacccaaac 1020
gcacattggagcctcacaatggcaagtgcaaggagctcttctccaaccaccagtgggtga 1080
gcgaggagagctgtgaggatgaggacatcctggccagagaggaaagcagagccccctcaa 1140
ttgctgtgcacagcatgagcagtatgagtcaagacactgaagttaggaaacggatggtgg 1200
gtggaggtcttcaaagtgctcaggcatctgtccccactgaggaagagctgtccccaaccg 1260
aggaggaacaaaaggcacacaggccagttcactggaagcccttcctggtcaacgtgtgca 1320
tggccacggccctggcgactggcgcgtacctctgttaccgggtatgttttcactgacaga 1380
ctgctgtgaggcatgagcgtggtgggcgctgccactgcccaggttaggatttggtctgcg 1440
gcgtctaacctggtgtagaagaaacaacagcttacaagcctgtggtggaactggaagggc 1500
cagccccaggaggggcatctgtgcactgggctttgaaggagcccctggtcccaagaacag 1560
agtctaatctcagggccttaacctgttcaggagaagtagaggaaatgccaaatactcttc 1620
ttgctctcacctcactcctcccctttctctggttcgtttgtttttggaaaaaaaaaaaaa 1680
gaattacaacacattgttgtttttaacatttataaaaaaaaaaaaaaaaaaaaaaaaaaa 1740
aaaa 1744
<210> 625
<211> 20
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: primer for psiCHECK insert
<400> 625
tgtccgcaac tacaacgcct 20
<210> 626
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 1, 2, 3, 5, 7, 8, 12, 14, 15, 16, 17
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 4, 6, 9, 10, 11, 13, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 626
cuuacgcuga guacuucgat t 21
<210> 627
<211> 21
<212> DNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<220>
<221> modified_base
<222> 1
<223> /mod_base = "nucleoside: lacks 5'-phosphate group"
<220>
<221> modified_base
<222> 7, 11, 16
<223> /mod_base = "2'-O-methyl corresponding nucleoside"
<220>
<221> modified_base
<222> 21
<223> /mod_base = "5'-phosphorothioate thymidine"
<220>
<221> modified_base
<222> 1, 2, 3, 4, 5, 6, 8, 9, 10, 12, 13, 14, 15, 17, 18, 19
<223> /mod_base = "2'-hydroxy corresponding nucleoside"
<400> 627
ucgaaguacu cagcguaagt t 21
<210> 628
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 628
augcauguaa ggucuucuu 19
<210> 629
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 629
aagaagaccu uacaugcau 19
<210> 630
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 630
aaacucgaga gaucuuaca 19
<210> 631
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 631
uguaagaucu cucgaguuu 19
<210> 632
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 632
ugauggagaa agguucguu 19
<210> 633
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 633
aacgaaccuu ucuccauca 19
<210> 634
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 634
ggagaaaggu ucguuaaaa 19
<210> 635
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 635
uuuuaacgaa ccuuucucc 19
<210> 636
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 636
aaaugaggaa guuucggau 19
<210> 637
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 637
auccgaaacu uccucauuu 19
<210> 638
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 638
auaucaacgc uaguuugau 19
<210> 639
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 639
aucaaacuag cguugauau 19
<210> 640
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 640
cacugaaguu agaagucgg 19
<210> 641
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 641
ccgacuucua acuucagug 19
<210> 642
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 642
cccuucuucc guugauauc 19
<210> 643
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 643
gauaucaacg gaagaaggg 19
<210> 644
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 644
ucgucaugcu caacagagu 19
<210> 645
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 645
acucuguuga gcaugacga 19
<210> 646
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 646
gauggagaaa gguucguua 19
<210> 647
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 647
uaacgaaccu uucuccauc 19
<210> 648
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 648
gaucuuacau uuccacuau 19
<210> 649
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 649
auaguggaaa uguaagauc 19
<210> 650
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 650
ucccuuugac cauagucgg 19
<210> 651
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 651
ccgacuaugg ucaaaggga 19
<210> 652
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 652
auagucggau uaaacuaca 19
<210> 653
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 653
uguaguuuaa uccgacuau 19
<210> 654
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 654
cuaguucuca aucacugcu 19
<210> 655
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 655
agcagugauu gagaacuag 19
<210> 656
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 656
cuagaucuag uucucaauc 19
<210> 657
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 657
gauugagaac uagaucuag 19
<210> 658
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 658
gaaguuagaa gucgggucg 19
<210> 659
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 659
cgacccgacu ucuaacuuc 19
<210> 660
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 660
cguacugguu uaaccuccu 19
<210> 661
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 661
aggagguuaa accaguacg 19
<210> 662
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 662
auuucaaaau ggacguacu 19
<210> 663
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 663
aguacgucca uuuugaaau 19
<210> 664
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 664
gaaugcaugu aaggucuuc 19
<210> 665
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 665
gaagaccuua caugcauuc 19
<210> 666
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 666
caugauguga gauuacuuu 19
<210> 667
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 667
aaaguaaucu cacaucaug 19
<210> 668
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 668
gguacagaga cgucagucc 19
<210> 669
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 669
ggacugacgu cucuguacc 19
<210> 670
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 670
uauuauacag ugcgacagc 19
<210> 671
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 671
gcugucgcac uguauaaua 19
<210> 672
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 672
auguuagaga gguagcgag 19
<210> 673
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 673
cucgcuaccu cucuaacau 19
<210> 674
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 674
ugcuauaugc cuuaagcca 19
<210> 675
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 675
uggcuuaagg cauauagca 19
<210> 676
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 676
ccuuaagcca auauuuacu 19
<210> 677
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 677
aguaaauauu ggcuuaagg 19
<210> 678
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 678
uuucaaaguc cgagaguca 19
<210> 679
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 679
ugacucucgg acuuugaaa 19
<210> 680
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 680
guuagagagg uagcgagcu 19
<210> 681
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 681
agcucgcuac cucucuaac 19
<210> 682
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 682
cacccaaacg aauccugga 19
<210> 683
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 683
uccaggauuc guuugggug 19
<210> 684
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 684
gacccuucuu ccguugaua 19
<210> 685
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 685
uaucaacgga agaaggguc 19
<210> 686
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 686
guacagagac gucaguccc 19
<210> 687
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 687
gggacugacg ucucuguac 19
<210> 688
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 688
acguacuggu uuaaccucc 19
<210> 689
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 689
ggagguuaaa ccaguacgu 19
<210> 690
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 690
cccuguuauc ugcuagauc 19
<210> 691
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 691
gaucuagcag auaacaggg 19
<210> 692
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 692
cucagacauu ccaagggua 19
<210> 693
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 693
uacccuugga augucugag 19
<210> 694
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 694
ccggacggac guugguucu 19
<210> 695
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 695
agaaccaacg uccguccgg 19
<210> 696
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 696
ugcgacagcu agaauugga 19
<210> 697
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 697
uccaauucua gcugucgca 19
<210> 698
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 698
acguggguau uuaauaaga 19
<210> 699
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 699
ucuuauuaaa uacccacgu 19
<210> 700
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 700
ugaccauagu cggauuaaa 19
<210> 701
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 701
uuuaauccga cuaugguca 19
<210> 702
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 702
uaucaacgcu aguuugaua 19
<210> 703
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 703
uaucaaacua gcguugaua 19
<210> 704
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 704
cuuuucaaag uccgagagu 19
<210> 705
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 705
acucucggac uuugaaaag 19
<210> 706
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 706
cacuauacca cauggccug 19
<210> 707
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 707
caggccaugu gguauagug 19
<210> 708
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 708
accugaucau uacauggcu 19
<210> 709
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 709
agccauguaa ugaucaggu 19
<210> 710
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 710
cagugacuuc ccauguaga 19
<210> 711
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 711
ucuacauggg aagucacug 19
<210> 712
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 712
gagauuacuu ugucccgcu 19
<210> 713
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 713
agcgggacaa aguaaucuc 19
<210> 714
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 714
ucaacgcuag uuugauaaa 19
<210> 715
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 715
uuuaucaaac uagcguuga 19
<210> 716
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 716
cauauuauac agugcgaca 19
<210> 717
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 717
ugucgcacug uauaauaug 19
<210> 718
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 718
uauguuagag agguagcga 19
<210> 719
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 719
ucgcuaccuc ucuaacaua 19
<210> 720
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 720
ccauagugca cuagcauuu 19
<210> 721
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 721
aaaugcuagu gcacuaugg 19
<210> 722
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 722
uugccuuucu cgugguaga 19
<210> 723
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 723
ucuaccacga gaaaggcaa 19
<210> 724
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 724
uaugccuuaa gccaauauu 19
<210> 725
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 725
aauauuggcu uaaggcaua 19
<210> 726
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 726
ccauagucgg auuaaacua 19
<210> 727
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 727
uaguuuaauc cgacuaugg 19
<210> 728
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 728
acccuucuuc cguugauau 19
<210> 729
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 729
auaucaacgg aagaagggu 19
<210> 730
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 730
auagguacag agacgucag 19
<210> 731
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 731
cugacgucuc uguaccuau 19
<210> 732
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 732
ucuuuucaaa guccgagag 19
<210> 733
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 733
cucucggacu uugaaaaga 19
<210> 734
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 734
uccauagugc acuagcauu 19
<210> 735
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 735
aaugcuagug cacuaugga 19
<210> 736
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 736
ugauauugug gguaacgug 19
<210> 737
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 737
cacguuaccc acaauauca 19
<210> 738
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 738
uuuaccagga uauccgaca 19
<210> 739
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 739
ugucggauau ccugguaaa 19
<210> 740
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 740
uuuucaaagu ccgagaguc 19
<210> 741
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 741
gacucucgga cuuugaaaa 19
<210> 742
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 742
cugaaguuag aagucgggu 19
<210> 743
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 743
acccgacuuc uaacuucag 19
<210> 744
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 744
uuugaguacc aaauccaca 19
<210> 745
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 745
uguggauuug guacucaaa 19
<210> 746
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 746
aaggguaugg gaagccaua 19
<210> 747
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 747
uauggcuucc cauacccuu 19
<210> 748
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 748
uaacacaugc ggucacuuu 19
<210> 749
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 749
aaagugaccg cauguguua 19
<210> 750
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 750
auggccaugg aauaaacca 19
<210> 751
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 751
ugguuuauuc cauggccau 19
<210> 752
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 752
uucuuccguu gauaucaag 19
<210> 753
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 753
cuugauauca acggaagaa 19
<210> 754
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 754
gugauauugu ggguaacgu 19
<210> 755
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 755
acguuaccca caauaucac 19
<210> 756
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 756
uacucaucag gucauuauu 19
<210> 757
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 757
aauaaugacc ugaugagua 19
<210> 758
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 758
uguggguaac gugagaaga 19
<210> 759
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 759
ucuucucacg uuacccaca 19
<210> 760
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 760
aacucgagag aucuuacau 19
<210> 761
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 761
auguaagauc ucucgaguu 19
<210> 762
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 762
ugcaugaccu gaucauuac 19
<210> 763
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 763
guaaugauca ggucaugca 19
<210> 764
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 764
guaguaguuu gggugugua 19
<210> 765
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 765
uacacaccca aacuacuac 19
<210> 766
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 766
cugguuuaac cuccuaucc 19
<210> 767
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 767
ggauaggagg uuaaaccag 19
<210> 768
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 768
auugugggua acgugagaa 19
<210> 769
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 769
uucucacguu acccacaau 19
<210> 770
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 770
uuagagaggu agcgagcug 19
<210> 771
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 771
cagcucgcua ccucucuaa 19
<210> 772
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 772
agugauauug uggguaacg 19
<210> 773
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 773
cguuacccac aauaucacu 19
<210> 774
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 774
uucuuuucaa aguccgaga 19
<210> 775
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 775
ucucggacuu ugaaaagaa 19
<210> 776
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 776
auggagaaag guucguuaa 19
<210> 777
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 777
uuaacgaacc uuucuccau 19
<210> 778
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 778
acucgagaga ucuuacauu 19
<210> 779
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 779
aauguaagau cucucgagu 19
<210> 780
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 780
ugcuagaucu aguucucaa 19
<210> 781
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 781
uugagaacua gaucuagca 19
<210> 782
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 782
ccuaacacau gcggucacu 19
<210> 783
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 783
agugaccgca uguguuagg 19
<210> 784
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 784
ggacguacug guuuaaccu 19
<210> 785
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 785
agguuaaacc aguacgucc 19
<210> 786
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 786
uuuacucauc aggucauua 19
<210> 787
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 787
uaaugaccug augaguaaa 19
<210> 788
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 788
gauuacuuug ucccgcuua 19
<210> 789
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 789
uaagcgggac aaaguaauc 19
<210> 790
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 790
uuagaagucg ggucguggg 19
<210> 791
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 791
cccacgaccc gacuucuaa 19
<210> 792
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 792
cuagcaggca ugccgcggu 19
<210> 793
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 793
accgcggcau gccugcuag 19
<210> 794
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 794
gauauugugg guaacguga 19
<210> 795
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 795
ucacguuacc cacaauauc 19
<210> 796
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 796
ucauauuaua cagugcgac 19
<210> 797
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 797
gucgcacugu auaauauga 19
<210> 798
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 798
gccuuucucg ugguagaag 19
<210> 799
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 799
cuucuaccac gagaaaggc 19
<210> 800
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 800
uugccuaaca caugcgguc 19
<210> 801
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 801
gaccgcaugu guuaggcaa 19
<210> 802
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 802
ucgaaggugc caaauucau 19
<210> 803
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 803
augaauuugg caccuucga 19
<210> 804
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 804
auauaaugaa cacgugggu 19
<210> 805
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 805
acccacgugu ucauuauau 19
<210> 806
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 806
accauagucg gauuaaacu 19
<210> 807
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 807
aguuuaaucc gacuauggu 19
<210> 808
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 808
uaugggaagc cauauucac 19
<210> 809
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 809
gugaauaugg cuucccaua 19
<210> 810
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 810
cacauuuugc cuuucucgu 19
<210> 811
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 811
acgagaaagg caaaaugug 19
<210> 812
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 812
gaucuaguuc ucaaucacu 19
<210> 813
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 813
agugauugag aacuagauc 19
<210> 814
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 814
ugaccugauc auuacaugg 19
<210> 815
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 815
ccauguaaug aucagguca 19
<210> 816
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 816
cauauucaca ccucacgcu 19
<210> 817
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 817
agcgugaggu gugaauaug 19
<210> 818
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 818
gugagauuac uuugucccg 19
<210> 819
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 819
cgggacaaag uaaucucac 19
<210> 820
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 820
cagaucgaca aguccggga 19
<210> 821
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 821
ucccggacuu gucgaucug 19
<210> 822
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 822
ugguuuaacc uccuauccu 19
<210> 823
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 823
aggauaggag guuaaacca 19
<210> 824
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 824
aagcuuccua agaacaaaa 19
<210> 825
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 825
uuuuguucuu aggaagcuu 19
<210> 826
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 826
cauuucaaaa uggacguac 19
<210> 827
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 827
guacguccau uuugaaaug 19
<210> 828
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 828
ccuccacauu aaguggcuu 19
<210> 829
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 829
aagccacuua auguggagg 19
<210> 830
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 830
acagugcgac agcuagaau 19
<210> 831
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 831
auucuagcug ucgcacugu 19
<210> 832
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 832
ugugagauua cuuuguccc 19
<210> 833
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 833
gggacaaagu aaucucaca 19
<210> 834
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 834
cuuugaccau agucggauu 19
<210> 835
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 835
aauccgacua uggucaaag 19
<210> 836
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 836
aucugcuaga ucuaguucu 19
<210> 837
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 837
agaacuagau cuagcagau 19
<210> 838
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 838
gacggacguu gguucugca 19
<210> 839
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 839
ugcagaacca acguccguc 19
<210> 840
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 840
gguucguuaa aaugcgcac 19
<210> 841
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 841
gugcgcauuu uaacgaacc 19
<210> 842
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 842
cugcuauaug ccuuaagcc 19
<210> 843
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 843
ggcuuaaggc auauagcag 19
<210> 844
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 844
uuucaaaaug gacguacug 19
<210> 845
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 845
caguacgucc auuuugaaa 19
<210> 846
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 846
uauaugccuu aagccaaua 19
<210> 847
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 847
uauuggcuua aggcauaua 19
<210> 848
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 848
gaccauaguc ggauuaaac 19
<210> 849
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 849
guuuaauccg acuaugguc 19
<210> 850
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 850
uacaacccaa gaaacucga 19
<210> 851
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 851
ucgaguuucu uggguugua 19
<210> 852
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 852
cuaacacaug cggucacuu 19
<210> 853
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 853
aagugaccgc auguguuag 19
<210> 854
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 854
ccggccaccc aaacgaauc 19
<210> 855
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 855
gauucguuug gguggccgg 19
<210> 856
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 856
gcgagcugcu cugcuauau 19
<210> 857
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 857
auauagcaga gcagcucgc 19
<210> 858
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 858
auuacuuugu cccgcuuau 19
<210> 859
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 859
auaagcggga caaaguaau 19
<210> 860
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 860
ccauauucac accucacgc 19
<210> 861
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 861
gcgugaggug ugaauaugg 19
<210> 862
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 862
acucaucagg ucauuauuu 19
<210> 863
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 863
aaauaaugac cugaugagu 19
<210> 864
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 864
auggugcuca cgacucuuc 19
<210> 865
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 865
gaagagucgu gagcaccau 19
<210> 866
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 866
ucugcuuacu aaugugccc 19
<210> 867
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 867
gggcacauua guaagcaga 19
<210> 868
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 868
agauuacuuu gucccgcuu 19
<210> 869
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 869
aagcgggaca aaguaaucu 19
<210> 870
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 870
aucgacaagu ccgggagcu 19
<210> 871
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 871
agcucccgga cuugucgau 19
<210> 872
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 872
gcaacucucc acuccauau 19
<210> 873
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 873
auauggagug gagaguugc 19
<210> 874
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 874
ucgagagauc uuacauuuc 19
<210> 875
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 875
gaaauguaag aucucucga 19
<210> 876
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 876
ccgacuagca ggcaugccg 19
<210> 877
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 877
cggcaugccu gcuagucgg 19
<210> 878
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 878
ugagauuacu uugucccgc 19
<210> 879
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 879
gcgggacaaa guaaucuca 19
<210> 880
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 880
gucggauuaa acuacauca 19
<210> 881
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 881
ugauguaguu uaauccgac 19
<210> 882
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 882
gccuaacaca ugcggucac 19
<210> 883
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 883
gugaccgcau guguuaggc 19
<210> 884
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 884
auuuacucau caggucauu 19
<210> 885
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 885
aaugaccuga ugaguaaau 19
<210> 886
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 886
agagguagcg agcugcucu 19
<210> 887
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 887
agagcagcuc gcuaccucu 19
<210> 888
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 888
gguaguacug ggaauggaa 19
<210> 889
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 889
uuccauuccc aguacuacc 19
<210> 890
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 890
auucacaccu cacgcucug 19
<210> 891
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 891
cagagcguga ggugugaau 19
<210> 892
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 892
aacacaugcg gucacuuuu 19
<210> 893
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 893
aaaagugacc gcauguguu 19
<210> 894
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 894
gcugcgucag accaguacu 19
<210> 895
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 895
aguacugguc ugacgcagc 19
<210> 896
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 896
auccuggagc cacacaaug 19
<210> 897
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 897
cauugugugg cuccaggau 19
<210> 898
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 898
cucccgagcu acucucuug 19
<210> 899
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 899
caagagagua gcucgggag 19
<210> 900
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 900
uuaggugcca ggcuguaag 19
<210> 901
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 901
cuuacagccu ggcaccuaa 19
<210> 902
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 902
gccuggguga cgguccuuu 19
<210> 903
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 903
aaaggaccgu cacccaggc 19
<210> 904
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 904
aguuagaagu cgggucgug 19
<210> 905
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 905
cacgacccga cuucuaacu 19
<210> 906
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 906
gaaagacccu ucuuccguu 19
<210> 907
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 907
aacggaagaa gggucuuuc 19
<210> 908
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 908
acacaugcgg ucacuuuug 19
<210> 909
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 909
caaaagugac cgcaugugu 19
<210> 910
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 910
uucaaaaugg acguacugg 19
<210> 911
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 911
ccaguacguc cauuuugaa 19
<210> 912
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 912
ccuuugacca uagucggau 19
<210> 913
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 913
auccgacuau ggucaaagg 19
<210> 914
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 914
ggaguucuuc ccaaaucac 19
<210> 915
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 915
gugauuuggg aagaacucc 19
<210> 916
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 916
auuuaccagg auauccgac 19
<210> 917
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 917
gucggauauc cugguaaau 19
<210> 918
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 918
ugaaguuaga agucggguc 19
<210> 919
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 919
gacccgacuu cuaacuuca 19
<210> 920
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 920
uccacuauac cacauggcc 19
<210> 921
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 921
ggccaugugg uauagugga 19
<210> 922
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 922
gugauggaga aagguucgu 19
<210> 923
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 923
acgaaccuuu cuccaucac 19
<210> 924
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 924
gugaggugug gauaaggcu 19
<210> 925
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 925
agccuuaucc acaccucac 19
<210> 926
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 926
gucagccuug caucaaggg 19
<210> 927
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 927
cccuugaugc aaggcugac 19
<210> 928
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 928
gugacggucc uuuaggcag 19
<210> 929
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 929
cugccuaaag gaccgucac 19
<210> 930
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 930
uauaccacau ggccugacu 19
<210> 931
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 931
agucaggcca ugugguaua 19
<210> 932
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 932
uauucacacc ucacgcucu 19
<210> 933
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 933
agagcgugag gugugaaua 19
<210> 934
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 934
ggugacgguc cuuuaggca 19
<210> 935
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 935
ugccuaaagg accgucacc 19
<210> 936
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 936
augucagccu ugcaucaag 19
<210> 937
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 937
cuugaugcaa ggcugacau 19
<210> 938
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 938
gcuaagagcg cgacgcggc 19
<210> 939
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 939
gccgcgucgc gcucuuagc 19
<210> 940
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 940
uuccacuaua ccacauggc 19
<210> 941
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 941
gccauguggu auaguggaa 19
<210> 942
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 942
auauuauaca gugcgacag 19
<210> 943
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 943
cugucgcacu guauaauau 19
<210> 944
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 944
agucggauua aacuacauc 19
<210> 945
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 945
gauguaguuu aauccgacu 19
<210> 946
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 946
agaucuuaca uuuccacua 19
<210> 947
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 947
uaguggaaau guaagaucu 19
<210> 948
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 948
gauggugcuc acgacucuu 19
<210> 949
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 949
aagagucgug agcaccauc 19
<210> 950
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 950
ccuuuaggca gccugccgc 19
<210> 951
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 951
gcggcaggcu gccuaaagg 19
<210> 952
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 952
gaccuccaca uuaaguggc 19
<210> 953
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 953
gccacuuaau guggagguc 19
<210> 954
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 954
uuauucuccu cccuguuau 19
<210> 955
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 955
auaacaggga ggagaauaa 19
<210> 956
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 956
gacguacugg uuuaaccuc 19
<210> 957
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 957
gagguuaaac caguacguc 19
<210> 958
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 958
gaaggagcuu ucccacgag 19
<210> 959
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 959
cucgugggaa agcuccuuc 19
<210> 960
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 960
gaaaaccuua caacccaag 19
<210> 961
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 961
cuuggguugu aagguuuuc 19
<210> 962
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 962
uaaauccuca gguaguacu 19
<210> 963
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 963
aguacuaccu gaggauuua 19
<210> 964
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 964
ggaucagccu ccgccauuc 19
<210> 965
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 965
gaauggcgga ggcugaucc 19
<210> 966
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 966
gguucaccuu gccgagaga 19
<210> 967
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 967
ucucucggca aggugaacc 19
<210> 968
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 968
ugauggugcu cacgacucu 19
<210> 969
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 969
agagucguga gcaccauca 19
<210> 970
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 970
uacauuucca cuauaccac 19
<210> 971
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 971
gugguauagu ggaaaugua 19
<210> 972
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 972
uacugguuua accuccuau 19
<210> 973
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 973
auaggagguu aaaccagua 19
<210> 974
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 974
gcagaucgac aaguccggg 19
<210> 975
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 975
cccggacuug ucgaucugc 19
<210> 976
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 976
gcaggcaugc cgcgguagg 19
<210> 977
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 977
ccuaccgcgg caugccugc 19
<210> 978
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 978
gcaugccgcg guagguaag 19
<210> 979
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 979
cuuaccuacc gcggcaugc 19
<210> 980
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 980
uugaccauag ucggauuaa 19
<210> 981
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 981
uuaauccgac uauggucaa 19
<210> 982
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 982
auaccacaug gccugacuu 19
<210> 983
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 983
aagucaggcc augugguau 19
<210> 984
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 984
uuugaccaua gucggauua 19
<210> 985
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 985
uaauccgacu auggucaaa 19
<210> 986
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 986
aaagacccuu cuuccguug 19
<210> 987
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 987
caacggaaga agggucuuu 19
<210> 988
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 988
aguucucaau cacugcucc 19
<210> 989
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 989
ggagcaguga uugagaacu 19
<210> 990
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 990
ucggauuaaa cuacaucaa 19
<210> 991
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 991
uugauguagu uuaauccga 19
<210> 992
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 992
uacagagacg ucagucccu 19
<210> 993
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 993
agggacugac gucucugua 19
<210> 994
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 994
cgcgacgcgg ccuagagcg 19
<210> 995
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 995
cgcucuaggc cgcgucgcg 19
<210> 996
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 996
aagguucguu aaaaugcgc 19
<210> 997
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 997
gcgcauuuua acgaaccuu 19
<210> 998
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 998
acgguccuuu aggcagccu 19
<210> 999
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 999
aggcugccua aaggaccgu 19
<210> 1000
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1000
uaggugccag gcuguaagc 19
<210> 1001
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1001
gcuuacagcc uggcaccua 19
<210> 1002
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1002
auuauacagu gcgacagcu 19
<210> 1003
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1003
agcugucgca cuguauaau 19
<210> 1004
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1004
aucuaguucu caaucacug 19
<210> 1005
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1005
cagugauuga gaacuagau 19
<210> 1006
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1006
cuuaaaugcc gcacccuac 19
<210> 1007
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1007
guagggugcg gcauuuaag 19
<210> 1008
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1008
agguucguua aaaugcgca 19
<210> 1009
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1009
ugcgcauuuu aacgaaccu 19
<210> 1010
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1010
caacacauag ccugacccu 19
<210> 1011
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1011
agggucaggc uauguguug 19
<210> 1012
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1012
ggcagccugc cgccgucuc 19
<210> 1013
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1013
gagacggcgg caggcugcc 19
<210> 1014
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1014
gaaacucgag agaucuuac 19
<210> 1015
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1015
guaagaucuc ucgaguuuc 19
<210> 1016
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1016
cuguagcucc cgagcuacu 19
<210> 1017
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1017
aguagcucgg gagcuacag 19
<210> 1018
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1018
caugccgcgg uagguaagg 19
<210> 1019
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1019
ccuuaccuac cgcggcaug 19
<210> 1020
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1020
gggcccuuug ccuaacaca 19
<210> 1021
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1021
uguguuaggc aaagggccc 19
<210> 1022
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1022
uucacaccuc acgcucugg 19
<210> 1023
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1023
ccagagcgug aggugugaa 19
<210> 1024
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1024
uagcucccga gcuacucuc 19
<210> 1025
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1025
gagaguagcu cgggagcua 19
<210> 1026
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1026
augccgcggu agguaaggg 19
<210> 1027
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1027
cccuuaccua ccgcggcau 19
<210> 1028
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1028
cauagucgga uuaaacuac 19
<210> 1029
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1029
guaguuuaau ccgacuaug 19
<210> 1030
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1030
ugccgcggua gguaagggc 19
<210> 1031
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1031
gcccuuaccu accgcggca 19
<210> 1032
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1032
gcgacgcggc cuagagcgg 19
<210> 1033
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1033
ccgcucuagg ccgcgucgc 19
<210> 1034
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1034
ggugcucacg acucuuccu 19
<210> 1035
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1035
aggaagaguc gugagcacc 19
<210> 1036
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1036
acuagcaggc augccgcgg 19
<210> 1037
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1037
ccgcggcaug ccugcuagu 19
<210> 1038
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1038
aaauagguac agagacguc 19
<210> 1039
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1039
gacgucucug uaccuauuu 19
<210> 1040
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1040
gaaacagccu gggugacgg 19
<210> 1041
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1041
ccgucaccca ggcuguuuc 19
<210> 1042
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1042
agcaggcaug ccgcgguag 19
<210> 1043
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1043
cuaccgcggc augccugcu 19
<210> 1044
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1044
gccauuuacc aggauaucc 19
<210> 1045
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1045
ggauauccug guaaauggc 19
<210> 1046
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1046
gcgcgacgcg gccuagagc 19
<210> 1047
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1047
gcucuaggcc gcgucgcgc 19
<210> 1048
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1048
ugccuaacac augcgguca 19
<210> 1049
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1049
ugaccgcaug uguuaggca 19
<210> 1050
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1050
cggccaccca aacgaaucc 19
<210> 1051
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1051
ggauucguuu ggguggccg 19
<210> 1052
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1052
gagacgucag ucccuuuga 19
<210> 1053
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1053
ucaaagggac ugacgucuc 19
<210> 1054
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1054
ggcaugccgc gguagguaa 19
<210> 1055
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1055
uuaccuaccg cggcaugcc 19
<210> 1056
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1056
agcgcgacgc ggccuagag 19
<210> 1057
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1057
cucuaggccg cgucgcgcu 19
<210> 1058
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1058
agacauucca aggguaugg 19
<210> 1059
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1059
ccauacccuu ggaaugucu 19
<210> 1060
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1060
ccacuauacc acauggccu 19
<210> 1061
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1061
aggccaugug guauagugg 19
<210> 1062
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1062
ccgccggacc gcguagaga 19
<210> 1063
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1063
ucucuacgcg guccggcgg 19
<210> 1064
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1064
uuugccuuuc ucgugguag 19
<210> 1065
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1065
cuaccacgag aaaggcaaa 19
<210> 1066
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1066
gaguucuucc caaaucacc 19
<210> 1067
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1067
ggugauuugg gaagaacuc 19
<210> 1068
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1068
agugacuucc cauguagag 19
<210> 1069
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1069
cucuacaugg gaagucacu 19
<210> 1070
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1070
gccacccaaa cgaauccug 19
<210> 1071
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1071
caggauucgu uuggguggc 19
<210> 1072
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1072
agcccacgcc cgacuagca 19
<210> 1073
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1073
ugcuagucgg gcgugggcu 19
<210> 1074
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1074
caggcaugcc gcgguaggu 19
<210> 1075
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1075
accuaccgcg gcaugccug 19
<210> 1076
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1076
ggccacccaa acgaauccu 19
<210> 1077
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1077
aggauucguu uggguggcc 19
<210> 1078
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1078
gagcccacgc ccgacuagc 19
<210> 1079
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1079
gcuagucggg cgugggcuc 19
<210> 1080
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1080
aacaaaaacc gaaauaggu 19
<210> 1081
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1081
accuauuucg guuuuuguu 19
<210> 1082
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1082
cgacuagcag gcaugccgc 19
<210> 1083
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1083
gcggcaugcc ugcuagucg 19
<210> 1084
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1084
uagguaaggg ccgccggac 19
<210> 1085
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1085
guccggcggc ccuuaccua 19
<210> 1086
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1086
cgcagagccc acgcccgac 19
<210> 1087
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1087
gucgggcgug ggcucugcg 19
<210> 1088
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1088
guaagggccg ccggaccgc 19
<210> 1089
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1089
gcgguccggc ggcccuuac 19
<210> 1090
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1090
agagcccacg cccgacuag 19
<210> 1091
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1091
cuagucgggc gugggcucu 19
<210> 1092
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1092
guggcuacgg uccucacgg 19
<210> 1093
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1093
ccgugaggac cguagccac 19
<210> 1094
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1094
auaaauccuc agguaguac 19
<210> 1095
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1095
guacuaccug aggauuuau 19
<210> 1096
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1096
aggcaugccg cgguaggua 19
<210> 1097
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1097
uaccuaccgc ggcaugccu 19
<210> 1098
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1098
gacuagcagg caugccgcg 19
<210> 1099
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1099
cgcggcaugc cugcuaguc 19
<210> 1100
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: sense strand of dsRNA
<400> 1100
gaaauaggua cagagacgu 19
<210> 1101
<211> 19
<212> RNA
<213> Artificial sequence
<220>
<223> Description of the Artificial sequence: antisense strand of dsRNA
<400> 1101
acgucucugu accuauuuc 19
Claims (22)
- 시험관내에서 PTP1B 유전자의 발현을 60% 이상, 바람직하게는 70% 이상, 가장 바람직하게는 80% 이상까지 억제할 수 있는 이중-가닥 리보핵산 분자.
- 제 1 항에 있어서,
이중-가닥 리보핵산 분자가 센스 가닥 및 센스 가닥에 적어도 부분적으로 상보성인 안티센스 가닥을 포함하고, 센스 가닥이 PTP1B를 암호화하는 mRNA의 적어도 일부와 90% 이상의 동일성을 갖는 서열을 포함하고, 상기 서열이 (i) 상기 안티센스 가닥에 대한 상기 센스 가닥의 상보성 영역에 위치하고; (ii) 길이가 30개 뉴클레오티드 미만인 이중-가닥 리보핵산 분자. - 제 1 항 또는 제 2 항에 있어서,
센스 가닥이 서열번호 630, 632, 634, 638, 640, 644 및 652에 나타낸 핵산 서열을 포함하고, 안티센스 가닥이 서열번호 631, 633, 635, 639, 641, 645 및 653에 나타낸 핵산 서열을 포함하며, 이중-가닥 리보핵산 분자가 서열번호 630/631, 632/633, 634/635, 638/639, 640/641, 644/645 및 652/653으로 이루어진 군에서 선택된 서열 쌍을 포함하는 이중-가닥 리보핵산 분자. - 제 3 항에 있어서,
안티센스 가닥이 길이가 1 내지 5개 뉴클레오티드, 바람직하게는 길이가 1 내지 2개 뉴클레오티드인 3' 돌출부를 추가로 포함하는 이중-가닥 리보핵산 분자. - 제 4 항에 있어서,
안티센스 가닥의 돌출부가 우라실, 또는 PTP1B를 암호화하는 mRNA에 상보성인 뉴클레오티드를 포함하는 이중-가닥 리보핵산 분자. - 제 3 항 내지 제 5 항 중 어느 한 항에 있어서,
센스 가닥이 길이가 1 내지 5개 뉴클레오티드, 바람직하게는 길이가 1 내지 2개 뉴클레오티드인 3' 돌출부를 추가로 포함하는 이중-가닥 리보핵산 분자. - 제 6 항에 있어서,
센스 가닥의 돌출부가 우라실, 또는 PTP1B를 암호화하는 mRNA와 동일한 뉴클레오티드를 포함하는 이중-가닥 리보핵산 분자. - 제 1 항 내지 제 7 항 중 어느 한 항에 있어서,
이중-가닥 리보핵산 분자가 하나 이상의 변형된 뉴클레오티드를 포함하는 이중-가닥 리보핵산 분자. - 제 8 항에 있어서,
변형된 뉴클레오티드가 2'-O-메틸 변형된 뉴클레오티드, 5'-포스포로티오에이트기를 포함하는 뉴클레오티드, 및 콜레스테릴 유도체 또는 도데카노산 비스데실아미드기에 연결된 말단 뉴클레오티드, 2'-데옥시-2'-플루오로 변형된 뉴클레오티드, 2'-데옥시-변형된 뉴클레오티드, 고정 뉴클레오티드, 염기결여 뉴클레오티드, 2'-아미노-변형된 뉴클레오티드, 2'-알킬-변형된 뉴클레오티드, 모르폴리노 뉴클레오티드, 포스포르아미데이트, 및 비-천연 염기 포함 뉴클레오티드로 이루어진 군에서 선택되는 이중-가닥 리보핵산 분자. - 제 8 항 또는 제 9 항에 있어서,
변형된 뉴클레오티드가 2'-O-메틸 변형된 뉴클레오티드, 5'-포스포로티오에이트기를 포함하는 뉴클레오티드, 및 데옥시티미딘인 이중-가닥 리보핵산 분자. - 제 3 항 내지 제 10 항 중 어느 한 항에 있어서,
센스 가닥 및/또는 안티센스 가닥이 1 내지 2개 데옥시티미딘의 돌출부를 포함하는 이중-가닥 리보핵산 분자. - 제 1 항 내지 제 11 항 중 어느 한 항에 있어서,
센스 가닥이 서열번호 3, 5, 7, 11, 13, 17 및 25에 나타낸 핵산 서열로 이루어진 군에서 선택되고, 안티센스 가닥이 서열번호 4, 6, 8, 12, 14, 18 및 26에 나타낸 핵산 서열로 이루어진 군에서 선택되며, 이중-가닥 리보핵산 분자가 서열번호 3/4, 5/6, 7/8, 11/12, 13/14, 17/18 및 25/26으로 이루어진 군에서 선택된 서열 쌍을 포함하는 이중-가닥 리보핵산 분자. - 제 1 항 내지 제 12 항 중 어느 한 항에 정의된 바와 같은 이중-가닥 리보핵산 분자에 포함되는 센스 가닥 및/또는 안티센스 가닥을 암호화하는 핵산 서열.
- 제 1 항 내지 제 12 항 중 어느 한 항에 정의된 바와 같은 이중-가닥 리보핵산 분자에 포함되는 센스 가닥 또는 안티센스 가닥 중 하나 이상을 암호화하는 뉴클레오티드 서열에 작동가능하게 연결된 조절 서열을 포함하거나, 또는 제 13 항의 핵산 서열을 포함하는 벡터.
- 제 1 항 내지 제 12 항 중 어느 한 항에 정의된 바와 같은 이중-가닥 리보핵산 분자, 제 13 항의 핵산 분자 또는 제 14 항의 벡터를 포함하는 세포, 조직 또는 비-인간 유기체.
- 제 1 항 내지 제 12 항 중 어느 한 항에 정의된 바와 같은 이중-가닥 리보핵산 분자, 제 13 항의 핵산 분자, 제 14 항의 벡터, 또는 제 15 항의 세포 또는 조직을 포함하는 약학 조성물.
- 제 16 항에 있어서,
약학적으로 허용되는 담체, 안정화제 및/또는 희석제를 추가로 포함하는 약학 조성물. - (a) 세포, 조직 또는 유기체에 제 1 항 내지 제 12 항 중 어느 한 항에 정의된 바와 같은 이중-가닥 리보핵산 분자, 제 13 항의 핵산 분자, 제 14 항의 벡터를 도입하고;
(b) 단계 (a)에서 생성된 세포, 조직 또는 유기체를 PTP1B 유전자의 mRNA 전사체의 분해를 야기하기에 충분한 시간동안 유지시켜 세포에서 PTP1B 유전자의 발현을 억제하는 단계
를 포함하는, 세포, 조직 또는 유기체에서 PTP1B 유전자의 발현을 억제하는 방법. - PTP1B 유전자의 발현에 의해 야기된 병리학적 상태 또는 질환의 치료, 예방 또는 관리를 필요로 하는 대상에게 치료 또는 예방 효과량의 제 1 항 내지 제 12 항 중 어느 한 항에 정의된 바와 같은 이중-가닥 리보핵산 분자, 제 13 항의 핵산 분자, 제 14 항의 벡터 및/또는 제 16 항 또는 제 18 항에 정의된 바와 같은 약학 조성물을 투여하는 것을 포함하는, PTP1B 유전자의 발현에 의해 야기된 병리학적 상태 또는 질환을 치료, 예방 또는 관리하는 방법.
- 제 19 항에 있어서,
대상이 인간인 방법. - 2형 당뇨병, 간부전, 비만, 이상지질혈증, 당뇨병성 죽상동맥경화증 또는 고혈압을 치료하는데 사용하기 위한, 제 1 항 내지 제 12 항 중 어느 한 항에 정의된 바와 같은 이중-가닥 리보핵산 분자, 제 13 항의 핵산 분자, 제 14 항의 벡터 및/또는 제 16 항 또는 제 18 항에 정의된 바와 같은 약학 조성물.
- 2형 당뇨병, 간부전, 비만, 이상지질혈증, 당뇨병성 죽상동맥경화증 또는 고혈압의 치료를 위한 약학 조성물을 제조하기 위한, 제 1 항 내지 제 12 항 중 어느 한 항에 정의된 바와 같은 이중-가닥 리보핵산 분자, 제 13 항의 핵산 분자, 제 14 항의 벡터 및/또는 제 15 항의 세포 또는 조직의 용도.
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EP2880167B1 (en) * | 2012-07-31 | 2018-04-18 | The Board of Regents of The University of Texas System | Methods and compositions for in vivo induction of pancreatic beta cell formation |
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- 2010-01-26 MX MX2011007776A patent/MX2011007776A/es not_active Application Discontinuation
- 2010-01-26 CN CN2010800064446A patent/CN102307996A/zh active Pending
- 2010-01-26 CA CA2750459A patent/CA2750459A1/en not_active Abandoned
- 2010-01-26 KR KR1020117017998A patent/KR20110100316A/ko not_active Application Discontinuation
- 2010-01-26 BR BRPI1008109-7A patent/BRPI1008109A2/pt not_active IP Right Cessation
- 2010-01-26 WO PCT/EP2010/050814 patent/WO2010089221A1/en active Application Filing
- 2010-01-26 SG SG2011054731A patent/SG173182A1/en unknown
- 2010-01-26 EP EP10701514A patent/EP2393926A1/en not_active Withdrawn
- 2010-01-26 AU AU2010211133A patent/AU2010211133A1/en not_active Abandoned
- 2010-01-29 US US12/696,100 patent/US20100197773A1/en not_active Abandoned
- 2010-02-02 AR ARP100100271A patent/AR075688A1/es unknown
- 2010-02-02 TW TW099103073A patent/TW201031425A/zh unknown
-
2011
- 2011-05-26 IL IL213147A patent/IL213147A0/en unknown
Also Published As
Publication number | Publication date |
---|---|
WO2010089221A1 (en) | 2010-08-12 |
EP2393926A1 (en) | 2011-12-14 |
AR075688A1 (es) | 2011-04-20 |
BRPI1008109A2 (pt) | 2015-08-25 |
CA2750459A1 (en) | 2010-08-12 |
IL213147A0 (en) | 2011-07-31 |
CN102307996A (zh) | 2012-01-04 |
AU2010211133A1 (en) | 2011-07-21 |
SG173182A1 (en) | 2011-09-29 |
TW201031425A (en) | 2010-09-01 |
US20100197773A1 (en) | 2010-08-05 |
MX2011007776A (es) | 2011-08-12 |
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