KR20110067813A - Mixed tea using leaf, root bark and fruit of mulberry tree - Google Patents

Abstract

PURPOSE: Mixed tea using leaves, root bark, and fruits of mulberry, and a producing method thereof are provided to offer the various pharmacological activities. CONSTITUTION: A producing method of mixed tea using leaves, root bark, and fruits of mulberry comprises the following steps: freeze-drying and pulverizing crushed mulberry leaves; freeze-drying crushed mulberry root bark; freeze-drying and crushing mulberry fruits; and mixing 40~60wt% of mulberry leaf powder, 35~55wt% of mulberry root bark powder, and 5wt% of mulberry fruit powder.

Description

Mixed Tea Using Leaf, Root Bark and Fruit of Mulberry Tree}

The present invention relates to a mixed tea (잎) using the leaves, root bark and fruit of the mulberry.

Mulberry is a rosewood mulberry plant ( Morus Alba , its leaves, roots, and fruits have been known for their pharmacological activity.

In Dongbogam, "Mulberry leaves are warm and nonpoisonous. They remove each species and water species, benefit large and small intestines, and get rid of wind pain." It repels the wind, removes the heat of the lungs, heals the fever, headache, and cough coming from the cold, removes phlegm, cleans the eyes and liver, treats eye diseases caused by yin and wind, and redness and dryness of the eyes. , Pain and heal. " According to recent research results, 1-DNJ (1-dcoxynojirimycin) and its derivatives, constipation improvement, heavy metal detoxification effect, edema, which have anti-hyperglycemic effect, anti-obesity effect, AIDS virus infection and anti-cancer effect It contains dietary fiber, which has an effect of improving lipid metabolism, GABA, which has a neurotransmitting effect, blood pressure lowering effect, and the like, and includes muberin and other routines having anti-inflammatory and diuretic effects. It is known that flavonoids such as Rutin and Quecetin are contained.

In the case of the mulberry roots, the bark (hereinafter called "Sangbaekpi") is in agreement with the "Bulge breathing 10 million (수) and dysentery edema (水 氣 浮腫), to remove the lungs of the lungs, benefit the capital (水道) and sea water And the blood that is mixed with saliva. "Recent studies show that moran A lowers blood sugar, Mulberfuran FG lowers blood pressure, and its anti-inflammatory effect. Umberiferon (umbeliferone) is contained, and it is also known to contain various bioactive substances such as morusin (morusin), mulberine, mulberrochromene (mulberrochromene).

In the case of mulberry fruit (hereinafter, "audi"), the consent is written "Black Audi is a mulberry tree spirits are gathered, it is beneficial to diabetes and five chapters. "It has the effect of replenishing the liver and kidneys and nurturing bleeding (energetic) and acts as a yangbyeolgi", and because of its various physiological activities, such as 쨈, jelly, juice, syrup, alcohol, etc. It is used as a material for food or various materials such as cosmetics.

The present invention has been completed in view of the development of tea with high preference by mixing mulberry leaves, baekbaekpi, Audi and the like having various pharmacological activities.

Accordingly, it is an object of the present invention to provide a mixed tea having a high degree of preference and having various pharmacological activities possessed by mulberry leaves, lettuce, and mulberry.

Other objects of the present invention will be presented below.

The present inventors attempted to make a mixed tea having optimal palatability by drying and mixing mulberry leaves, lettuce, and mulberry having various pharmacological activities as described above, as confirmed in the following Reference Examples, Examples and Experimental Examples. In the process, the present invention was completed by confirming that the palatability is high and low according to a specific mixing ratio.

The mixed tea of the present invention includes the dried mulberry leaf of (a) below, the dried baekpipi of (b) below and the dried audio of (c) below, the dried mulberry leaves 40% to 60% by weight, dried Morus bark is characterized in that it comprises from 30% to 50% by weight, and dried audio from 5% to 10% by weight.

(a) dried mulberry leaves obtained by freeze-drying the mulberry leaf mill,

(b) dried dry white skin obtained by lyophilizing the ground powder of dry white skin, and

(c) dried audio obtained by lyophilization and grinding of the audio.

As confirmed in the following Examples and Experimental Examples, the mixed tea of 30% by weight of dried mulberry leaves, 60% by weight of dried phase white skin and 10% by weight of dried mulberry when the sensory evaluation results using only dried mulberry leaves Lower than 60% by weight of dried mulberry leaves, 20% by weight of dried mulberry leaves and 10% by weight of dried mulberry tea, 30% by weight of dried mulberry leaves, 65% by weight of dried mulberry leaves and 5% of dried mulberry leaves There was no significant difference in the sensory evaluation of the mixed tea of 70% by weight, the dried mulberry leaf, the dried mulberry leaf 25% by weight, and the dried mulberry 5% by weight of the dried mulberry leaves. . These results can be said that there is a difference in the degree of preference according to the mixing ratio of dried mulberry leaf, dried lettuce and dried mulberry, the mixing ratio of the dried mulberry leaves, dried lettuce and dried mulberry It is determined based on.

In a preferred aspect, the mixed tea of the present invention is a case where the dried mulberry leaves 40% to 60% by weight, dried baekpipi 35% to 55% by weight, and 5% by weight of the audio. This is because the sensory evaluation results of the following experimental examples were the best in these mixing ratios (see <Example 7> to <Example 9> items in Table 2 below).

In the present invention, the mulberry leaves, baekryepi and the like is used to lyophilized to prevent the loss of the functional components of the mulberry leaves, baekryepi and the like by heat. For example, it is reported that 50% of mulberry leaves are destroyed by steaming at 80 ° C. for 1 minute, and 46% of Gabba (Rural Development Administration, Functional Sleep, 2005). As confirmed by the experimental example below, the mulberry leaf tea obtained through the rice cake process and the mindful process according to the conventional manufacturing process of tea and the mulberry leaf tea obtained by lyophilization as in the present invention, there was no significant difference in preference. In the case of the mixed tea of the invention, the palatability was significantly higher than that of the mulberry leaf tea obtained through the rice cake process and the mind process. These results can be said that even when using the lyophilized mulberry leaves, such as tea can be obtained to preserve the functional ingredients while not dropping or increasing the preference.

In the present invention, before the lyophilization of the mulberry leaf pulverized (a) of the mulberry leaf pulverized in a vacuum filled with CO ₂ and the temperature is maintained at 20 ℃ to 28 ℃ anaerobic for about 7 hours to 10 hours It is preferable to perform the treatment. It is not shown in the following experimental example, but according to the experience of the present inventors, it is because there is an effect that the functional components such as the routine of the mulberry leaves, Gaba, etc. are not lost and preserved.

In the present invention, it is preferable that the moisture content of the dried mulberry leaves, dried baekpipi and dried mulberry is 20% (wt% based on the total weight of dried mulberry leaves, dried baekpipi or dried mulberry; the same as below) . In particular, when the moisture content is high, unwanted fermentation may occur during the storage or distribution process of the tea, so that the original intended taste may be modified, and experience shows that the tea has an excellent taste and aroma only when the moisture content is appropriately low. It is preferable that the moisture content is 10% or less. The lower limit of the moisture content is not particularly limited, but in general, the moisture content of the tea is 3% or more, and in the case where the moisture content of the tea is extremely low, the functional component contained therein may be destroyed. It is preferable that it is 3% or more. The moisture content can be controlled by controlling the moisture content of raw materials such as mulberry leaf pulverization used for lyophilization, the temperature of the lyophilization, the lyophilization time, and the like. Can be. The present inventors lyophilized at -40 ℃ for 24 hours in the case of dried mulberry leaf in the following example to the water content of 6%, in the case of dried baekpipi lyophilized at -40 ℃ for 24 hours to 7% moisture content In the case of dried Audi, the moisture content was 7% by freeze drying at -40 ° C for 30 hours.

In the blended tea of the present invention, the average particle size of the pulverized product is not particularly limited, but the average particle size is preferably 5 mm or less in order to facilitate leaching of components contained in the tea raw material such as dried mulberry leaves. The ground product may have a particle size of 50 mesh or less, preferably 100 mesh or less, when the blended tea of the present invention is sold in powder form.

The mixed tea of the present invention can also be used in the form of tea bags, powders, leachate, and the like. In the form of leachate, it will be packaged in a container such as a can and distributed.

In another aspect, the present invention relates to a hangover tea composition for the hangover comprising a mixture of dried mulberry leaves of (a), dried baekpipi (b), and dried (i) of the (c) as an active ingredient will be.

Hangovers, such as nausea and headaches after drinking too much, are caused by aldehydes in alcohol metabolism. In the presence of alcoholase (ADH) and aldehydease (ALDH) in the liver in general, when alcohol enters the body, the alcohol is converted to acetaldehyde by ADH, and acetaldehyde is converted to acetate, which is harmless to the body by ALDH. do.

As can be seen in the following examples, the leaching solution of the mixed tea of the present invention can be usefully used for hangover relief in that it promotes ADH activity and ALDH activity. In particular, as can be seen in the experimental example below, the ADH promoting activity and ALDH promoting activity of the leaching solution of the mixed tea of the present invention is superior to the ADH promoting activity and ALDH promoting activity of the leaching solution of each single tea used in the mixed tea. High. This shows that the mixed tea of the present invention may be more useful for hangover use than each single tea.

In the hangover tea composition of the present invention, the dried mulberry leaf is 40% to 60% by weight, the dried baekpipi 30% by weight to 50% by weight, and dried audio is contained in 5% to 10% by weight Preferably, mulberry leaf is included 40 wt% to 60 wt%, dried baekpipi is 35 wt% to 55 wt%, and preferably comprises 5 wt% of Audi. In this case, it is possible to provide a tea composition having high hangover resolution and high preference.

The hangover tea composition of the present invention may also be in the form of tea bags, powders, or the like, and may also be in the form of leach liquors packed in containers such as cans.

As described above, according to the present invention, it is possible to provide a mixed tea having various pharmacological activities and high preference using mulberry leaves, lettuce, and mulberry. In addition, according to the present invention can provide a tea composition for hangover relief. Such mixed tea or tea composition may be commercialized in the form of a tea bag, powder, leachate packaged in a container such as a can.

Hereinafter, the present invention will be described with reference to reference examples, examples, and experimental examples. However, the scope of the present invention is not limited to these reference examples, examples and experimental examples.

< Reference Example > Preparation of Dried Mulberry Leaves, Dried Morus and Dried Audi

Reference Example 1 Preparation of Dried Mulberry Leaves

The mulberry leaf pulverized particles having a particle size of about 2 to 5 mm were placed in a sealed incubator and filled with CO ₂ gas in a vacuum without air, followed by anaerobic treatment for 8 hours at 25 ° C., followed by freeze-drying at -40 ° C. for 24 hours. Dried mulberry leaves were prepared. Moisture content was 6% by weight.

Reference Example 2 Preparation of Dried Cemented Peel

After cooling the baekpipipi crushed powder having a particle size of about 2 ~ 5mm for 3 minutes at a temperature of 75 ℃ ~ 80 ℃ and lyophilized for 24 hours in the range -40 ℃ to prepare a dried baekpipi. Moisture content was 7% by weight.

Reference Example 3 Preparation of Dried Audi

The audio was lyophilized for 30 hours in the range of -40 ° C. and then pulverized using a grinder to prepare dried audio having a particle size of approximately 2 to 5 mm. Moisture content was 7% by weight.

< Example > Preparation of Mixed Tea Using Dried Mulberry Leaves, Dried White Cortex and Dried Audi

Tea was prepared by mixing dried mulberry leaves of <Reference Example 1>, dried baekbaekpi of <Reference Example 2> and dried Audi of <Reference Example 3 in the ratio of Table 1 below.

TABLE 1

Mixing ratio

division Dried Mulberry Leaves (% by weight) Dried Morus Bark (% by weight) Dried Audi (% by weight) Example 1 30 60 10 Example 2 40 50 10 Example 3 50 40 10 Example 4 60 30 10 Example 5 70 20 10 Example 6 30 65 5 Example 7 40 55 5 Example 8 50 45 5 Example 9 60 35 5 Example 10 70 25 5

< Experimental Example > Sensory Evaluation and Hangover Relief Activity for Palatability

Experimental Example 1 Sensory Evaluation on Palatability

The mixed tea (10 mg) prepared in the above Example was leached for about 3 minutes by adding water (80 ml) at about 85 ° C., and the taste, color, and aroma of 20 male and female college students were evaluated for palatability. The degree of preference was evaluated. The evaluation score of the preference was very good (5 points), excellent (3 points), moderate (0 points), bad (-3 points), very bad (-5 points) (high score 100 points (5 points X 20) ) And the lowest score is -100 points (-5 points X 20).

As a comparative example, the dried mulberry leaf of <Reference Example 1> was leached with water at about 85 ° C. for 3 minutes (Comparative Example 1), and the rice cake process was performed for 40 minutes at 150 rpm in a steaming machine having a temperature range of 120 ° C. to 200 ° C. ) And mulberry leaf tea (Comparative Example 2) obtained through the mind process (performed for 10 minutes with a rotating heart of 150 ℃) was used.

TABLE 2

Sensory evaluation result of preference

division Evaluation value (mean ± standard deviation) Comparative Example 1
(Control group) 17.67 ± 9.52 Comparative Example 2 19.33 ± 10.73 Example 1 8.67 ± 7.45 Example 2 32.33 ± 9.2 * Example 3 45.00 ± 6.28 ** Example 4 36.33 ± 11.52 ** Example 5 21.33 ± 9.32 Example 6 19.00 ± 5.72 Example 7 52.67 ± 12.32 ** Example 8 55.67 ± 9.24 ** Example 9 48.67 ± 7.45 ** Example 10 25.33 ± 10.24 * p <0.05, p <0.01

The statistical significance test among the experimental groups was performed at Duncan's multiple range test at the 1% and 5% level of confidence.

The result of Table 2 shows that the mixing difference of <Example 2> to <Example 4> and the mixing difference of <Example 7> to <Example 9> is statistically significant, and the overall sensory evaluation results Also shown to be significantly higher than the control group (particularly the mixing difference of <Example 7> to <Example 9>). In the case of <Example 1> rather, the sensory evaluation result was lower than that of the comparative example, and thus it shows that the sensory evaluation result depends on the mixing ratio when mixing mulberry leaves, lettuce, and mulberry. In the case of <Example 6> and <Example 10>, although the sensory evaluation result was high compared with the non-control group, there was no significance.

On the other hand, there was no statistical difference between the mulberry leaf tea obtained by lyophilization of <Comparative Example 1> and the mulberry leaf tea obtained through the rice cake and mindful process of <Comparative Example 2> and there was no particular difference in the sensory evaluation results.

Experimental Example 2 Experiment on Hangover Relief Activity

In the above example, the ADH activity and ALDH activity were modified by modifying the method of Bostian et al. (Bostian et al., Biochem J., 173 (3): 773-86 (1978)) to promote the alcohol degradation activity of the mixed tea. It evaluated by measuring.

The reaction mixture for the analysis of ADH activity was 1.5 ml of leachate of the same amount of the mixed tea of <Reference Examples 1 to 3> (in the same manner as in <Experimental Example 1>, 80 ml of water was added to the 10 mg mixed tea). , 0.75 ml of 1M Tris-HCl (pH 8.8), 0.3 ml of 20 mM NAD +, 0.3 ml of ethanol, and 0.15 ml of ADH fraction (homogenous mouse liver cell), the reaction mixture for ALDH activity assay 2.2 mL of the same amount of leachate of the mixed tea of <Reference Examples 1 to 3> (80 mL of water was added to the 10 mg mixed tea in the same manner as in <Experimental Example 1>), and 0.3 ml of 1M Tris-HCl (pH 8.8), 0.1 ml 20mM NAD +, 0.1 ml 1.0M acetaldehyde, 0.1 ml 3M KCl, 0.1 ml 0.33 M 2-mercaptoethanol and 0.1 ml ALDH fraction (homogenous mouse liver cells) consist of.

After incubation at 30 ° C. for 5 minutes, the change in absorbance at 340 nm was measured, and the results are shown in the following Table 3 as the relative activity of the control group (100%).

On the other hand, as a comparative example, the dried mulberry leaves, dried lettuce and dried mulberry of each reference example was leached by adding 80 ml of water to 10 mg dried mulberry leaves, dried lettuce and dried mulberry in the same manner as in <Experimental Example 1> ADH and ALDH activity were evaluated.

[Table 3]

Activity of ADH and ALDH

division ADH activity ALDH Mixed tea 127.7% 136.3% Unity of <Comparative Example 1> 113.6% 117.2% Unity of <Comparative Example 2> 98.2% 103.4% Unity of <Comparative Example 3> 102.3% 95.2%

The results of Table 3 indicate that the leaching tea of the mixed tea can be usefully used for hangover relief. In particular, the ADH and ALDH promoting activity of the mixed tea is significantly higher than the ADH and ALDH promoting activity of a single tea.

Claims (9)

The dried mulberry leaf of (a) below, the dried baekpipi of (b) below, and the dried Audi of (c) below, wherein the dried mulberry leaves are 40% to 60% by weight, the dried baekbaekpi % To 50% by weight, and dried tea is mixed with 5 to 10% by weight of mixed mulberry leaves, root bark and berries (a) dried mulberry leaves obtained by freeze-drying the mulberry leaf mill, (b) dried dry white skin obtained by lyophilizing the ground powder of dry white skin, and (c) dried audio obtained by lyophilization and grinding of the audio. The method of claim 1, The dried mulberry leaves are 40% to 60% by weight, the dried lettuce leaves are 35% to 55% by weight, and the dried mulberry leaves, root bark and fruit of mulberry, characterized in that it comprises 5% by weight. Tea by using a mixture. The method of claim 1, The mulberry leaf pulverized product of (a) is mulberry leaf treated anaerobically for 7 hours to 10 hours in a reactor in which the mulberry leaf pulverized is filled with CO ₂ in a vacuum state and the temperature is maintained at 20 ℃ to 28 ℃ before lyophilization Mixed tea using leaves, root bark and berries of mulberry, characterized in that the powder. The method of claim 1, The dried mulberry leaves, dried baekbaekpi and dried mulberry mixed tea using leaves, root bark and berries of the mulberry, characterized in that the moisture content of all 3% or more and 10% or less. The method of claim 1, The mixed tea is a tea bag, powder or mixed tea using leaves, root bark and fruit of mulberry, characterized in that the form of leaching liquid packaged in a container. Hangover tea composition comprising the dried mulberry leaves of (a) below, the dried baekpipi (b) below and the dried audio of (c) below (a) dried mulberry leaves obtained by freeze-drying the mulberry leaf mill, (b) dried dry white skin obtained by lyophilizing the ground powder of dry white skin, and (c) dried audio obtained by lyophilization and grinding of the audio. The method of claim 6, The dried mulberry leaves 40% by weight to 60% by weight, the dried baekbaekpi 30% by weight to 50% by weight, and the dried mulberry tea for the hangover, characterized in that it comprises 5% to 10% by weight Composition. The method of claim 6, The dried mulberry leaves 40% by weight to 60% by weight, the dried baekbaekpi 35% by weight to 55% by weight, and the dried audi tea composition for hangover, characterized in that it comprises 5% by weight. The method of claim 1, The tea composition is a tea bag form, powder form or tea composition for hangover relief, characterized in that in the form of a leachate packaged in a container.