CN110882175A - Extraction method and application of quinoa saponin - Google Patents
Extraction method and application of quinoa saponin Download PDFInfo
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- CN110882175A CN110882175A CN201911243585.9A CN201911243585A CN110882175A CN 110882175 A CN110882175 A CN 110882175A CN 201911243585 A CN201911243585 A CN 201911243585A CN 110882175 A CN110882175 A CN 110882175A
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- 240000006162 Chenopodium quinoa Species 0.000 title claims abstract description 128
- 229930182490 saponin Natural products 0.000 title claims abstract description 69
- 150000007949 saponins Chemical class 0.000 title claims abstract description 69
- 239000001397 quillaja saponaria molina bark Substances 0.000 title claims abstract description 63
- 238000000605 extraction Methods 0.000 title claims abstract description 27
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 52
- 238000000034 method Methods 0.000 claims abstract description 19
- 238000009210 therapy by ultrasound Methods 0.000 claims abstract description 16
- 238000001035 drying Methods 0.000 claims abstract description 13
- 238000007873 sieving Methods 0.000 claims abstract description 13
- 238000005374 membrane filtration Methods 0.000 claims abstract description 9
- 235000017709 saponins Nutrition 0.000 claims description 64
- 235000015493 Chenopodium quinoa Nutrition 0.000 claims description 39
- 239000000047 product Substances 0.000 claims description 22
- 239000000843 powder Substances 0.000 claims description 20
- 239000000243 solution Substances 0.000 claims description 16
- 241000209140 Triticum Species 0.000 claims description 12
- 235000021307 Triticum Nutrition 0.000 claims description 12
- 239000011259 mixed solution Substances 0.000 claims description 12
- 239000006228 supernatant Substances 0.000 claims description 12
- 235000013339 cereals Nutrition 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 8
- 239000000287 crude extract Substances 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 238000003306 harvesting Methods 0.000 claims description 6
- 239000012528 membrane Substances 0.000 claims description 6
- 239000011148 porous material Substances 0.000 claims description 6
- 238000012216 screening Methods 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 6
- 238000003860 storage Methods 0.000 claims description 6
- 238000007710 freezing Methods 0.000 claims description 2
- 230000008014 freezing Effects 0.000 claims description 2
- 239000012982 microporous membrane Substances 0.000 claims description 2
- 238000005119 centrifugation Methods 0.000 claims 2
- 239000002537 cosmetic Substances 0.000 claims 1
- 238000002604 ultrasonography Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 abstract description 10
- 230000003796 beauty Effects 0.000 abstract description 8
- 230000003020 moisturizing effect Effects 0.000 abstract description 7
- 238000000926 separation method Methods 0.000 abstract description 4
- 230000008591 skin barrier function Effects 0.000 abstract description 4
- 206010016807 Fluid retention Diseases 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 229940079593 drug Drugs 0.000 abstract description 2
- 238000001471 micro-filtration Methods 0.000 abstract description 2
- 238000002137 ultrasound extraction Methods 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 2
- 210000003491 skin Anatomy 0.000 description 19
- 235000013312 flour Nutrition 0.000 description 14
- 230000000694 effects Effects 0.000 description 11
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- 229910052500 inorganic mineral Inorganic materials 0.000 description 7
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- 238000010438 heat treatment Methods 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 210000002510 keratinocyte Anatomy 0.000 description 4
- 230000001737 promoting effect Effects 0.000 description 4
- 230000002087 whitening effect Effects 0.000 description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- 102100028314 Filaggrin Human genes 0.000 description 3
- 101710088660 Filaggrin Proteins 0.000 description 3
- 102100031784 Loricrin Human genes 0.000 description 3
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 108010079309 loricrin Proteins 0.000 description 3
- 239000011777 magnesium Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 210000000434 stratum corneum Anatomy 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
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- 229910052725 zinc Inorganic materials 0.000 description 2
- 241000219317 Amaranthaceae Species 0.000 description 1
- 241000554155 Andes Species 0.000 description 1
- YDNKGFDKKRUKPY-JHOUSYSJSA-N C16 ceramide Natural products CCCCCCCCCCCCCCCC(=O)N[C@@H](CO)[C@H](O)C=CCCCCCCCCCCCCC YDNKGFDKKRUKPY-JHOUSYSJSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- CRJGESKKUOMBCT-VQTJNVASSA-N N-acetylsphinganine Chemical compound CCCCCCCCCCCCCCC[C@@H](O)[C@H](CO)NC(C)=O CRJGESKKUOMBCT-VQTJNVASSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 244000187664 Nerium oleander Species 0.000 description 1
- 241001127637 Plantago Species 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 206010039897 Sedation Diseases 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000004458 antinutrient Nutrition 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 229910001576 calcium mineral Inorganic materials 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 229940106189 ceramide Drugs 0.000 description 1
- ZVEQCJWYRWKARO-UHFFFAOYSA-N ceramide Natural products CCCCCCCCCCCCCCC(O)C(=O)NC(CO)C(O)C=CCCC=C(C)CCCCCCCCC ZVEQCJWYRWKARO-UHFFFAOYSA-N 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 235000021588 free fatty acids Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000001965 increasing effect Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229910001607 magnesium mineral Inorganic materials 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- VVGIYYKRAMHVLU-UHFFFAOYSA-N newbouldiamide Natural products CCCCCCCCCCCCCCCCCCCC(O)C(O)C(O)C(CO)NC(=O)CCCCCCCCCCCCCCCCC VVGIYYKRAMHVLU-UHFFFAOYSA-N 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000036280 sedation Effects 0.000 description 1
- 230000005808 skin problem Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 150000008130 triterpenoid saponins Chemical class 0.000 description 1
- 229910001656 zinc mineral Inorganic materials 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/27—Zinc; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/16—Emollients or protectives, e.g. against radiation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
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Abstract
The invention discloses an extraction method and application of quinoa saponin, which comprises the following steps: quinoa seeds, drying, crushing, sieving, adding ethanol to adjust the feed-liquid ratio, carrying out ultrasonic treatment, centrifuging, carrying out membrane filtration, carrying out quinoa saponin extracting solution, and refrigerating and storing in dark place; the invention adopts an ethanol ultrasonic extraction method, combines centrifugal separation and microfiltration membrane filtration, has simple operation steps and high saponin purity, and the quinoa saponin can promote the expression of natural moisturizing factors, promote the water retention of skin tissues and strengthen skin barriers, thereby ensuring that the skin moisturizing effect of the water in the skin is excellent and being widely applied to the field of medicines or beauty and skin care products.
Description
Technical Field
The invention relates to the technical field of separation and extraction of plant components, in particular to an extraction method and application of quinoa saponin.
Background
Chenopodium quinoa, also known as Chenopodium quinoa, Indian wheat, quinoa and quinoa, belongs to Amaranthaceae, an annual herb. Chenopodium quinoa is originally produced in Andes mountain range in Bolivia, Chile and Peru, is gradually popularized as a health care product in North America and Europe markets, and in recent years, is planted in large areas in Shanxi, Jilin, Qinghai, Gansu, Hebei and other places in China. Chenopodium quinoa is the first choice space food for American astronauts and the only total nutrient food recommended by the food and agriculture organization of the United nations, and is an internationally recognized total nutrient perfect food. The quinoa is rich in saponin, mainly exists in the form of oleander type triterpenoid saponin, the content of mature quinoa seed saponin is about 20% -30%, the content of seed coat saponin accounts for 80% of total saponin, so that the quinoa seed coat is slightly bitter and astringent, and is a main anti-nutrient substance in the quinoa. The quinoa saponin is a natural mild plant surfactant, has antibacterial activity and medicinal values of fever reduction, sedation, cancer resistance, oxidation resistance and the like, wherein the red quinoa saponin has high content and strong oxidation resistance, and can be obtained by extraction, separation and other technologies.
The skin stratum corneum lipid mainly contains 40% of ceramide, 25% of cholesterol and 25% of free fatty acid. The reason why the skin can be kept non-dry and non-rough is because the natural moisturizing factor NMF, mainly amino acids and lactate, minerals, etc., exists in the stratum corneum of the skin. The applicant finds that the nutritional value of the quinoa is closely related to the nutrients required by the horny layer of human skin, so that the application of the quinoa in beauty and skin care products is significant.
Disclosure of Invention
The invention aims to provide an extraction method and application of quinoa saponin, which aims to solve the problems in the prior art, fully utilizes and develops whitening and skin-care effects of quinoa saponin by extracting the quinoa saponin, applies the quinoa saponin to whitening and skin-care products to produce safe and mild natural skin-care products, and provides product processing raw materials for beautifying and skin-care while fully utilizing resources.
In order to achieve the purpose, the invention provides the following scheme:
on one hand, the invention provides a method for extracting quinoa saponin, which comprises the following steps:
(1) harvesting chenopodium quinoa seeds: taking fresh harvested chenopodium quinoa willd, and peeling chenopodium quinoa willd seeds;
(2) and (3) drying: screening quinoa grains, and drying;
(3) crushing and sieving: crushing chenopodium quinoa seeds, wherein a flour mill cannot be operated for a long time, so that the heating temperature of the flour mill is prevented from being too high to influence the later-stage extraction effect, sieving the chenopodium quinoa seed powder with a 60-mesh sieve, collecting undersize products, placing the undersize products in a wide-mouth bottle for sealing, and storing the sealed chenopodium quinoa seeds in a cool and dry place for later use;
(4) adding ethanol and mixing: mixing quinoa seed powder with ethanol solution;
(5) ultrasonic treatment: carrying out ultrasonic treatment on the mixed solution of the quinoa wheat seed powder and ethanol;
(6) centrifuging: centrifuging the mixed solution after ultrasonic treatment, and taking supernatant;
(7) membrane filtration: filtering the supernatant with organic microporous membrane to obtain filtrate as rhizoma et radix Veratri Sativi saponin extractive solution;
(8) and (4) light-resistant cold storage: freezing the crude extract of quinoa saponin at low temperature and storing in dark place for later use.
Further, the ethanol solution has a volume concentration of 75%.
Further, the volume ratio of the quinoa wheat seed powder to the ethanol solution is 1: 15-25.
Further, the ultrasonic power is 350-450W.
Further, the ultrasonic time is 50-70 min.
Further, the ultrasonic temperature is 50-70 ℃.
Further, the rotation speed of the centrifugal treatment is 2600-3000 r/min.
Further, the centrifugal processing time is 3-8 min.
Further, the pore diameter of the organic microporous filter membrane is 0.22 μm.
On the other hand, the invention provides the application of the quinoa saponin extracted by the extraction method of the quinoa saponin in a beauty and skin care product.
Furthermore, the addition amount of the quinoa saponin in the beauty skin care product is 0.01-4.5 wt%, and substances added together with the quinoa saponin can also comprise one or more than two mineral components of calcium, iron, magnesium and zinc, wherein the addition amount of the mineral components is 0.01-0.09 wt%.
Further, the mineral components are calcium chloride, ferrous sulfate, magnesium sulfate and zinc sulfate.
The invention discloses the following technical effects:
the invention adopts the ethanol ultrasonic extraction method, combines centrifugal separation and microfiltration membrane filtration, has simple operation steps and high saponin purity, and can be widely applied to the field of medicines or beauty and skin care products.
The quinoa saponin extracted by the extraction method provided by the invention has high purity which can reach more than 80%, has no impurities, and the extraction rate of the extraction method is high and can reach 4-8%.
The quinoa saponin can promote the expression of natural moisturizing factors, promote the water retention of skin tissues and strengthen the skin barrier, so that the skin moisturizing effect of the water in the skin is excellent.
The addition amount of quinoa saponin can promote the increase of filaggrin expression according to mineral components, promote the skin moisture preservation, and promote the increase of loricrin expression according to mineral components, thereby promoting the effect of strengthening the skin barrier. When the quinoa saponin is less than 0.01 wt% based on the total weight of the beauty skin care product, it is difficult to make the expression promoting effect of filaggrin and loricrin, and when it exceeds 4.5 wt%, there is a difficulty in the preparation of stability or dosage form.
In a word, by using the quinoa saponin as a raw material for processing and producing the beauty and skin care product, the beauty and skin care product has excellent oxidation resistance, good skin whitening effect, antibacterial effect, skin problem improving effect and skin color regulating effect by promoting blood circulation, thereby having good skin whitening and skin care effects.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The extraction method of the quinoa saponins is characterized by comprising the following steps:
(1) harvesting chenopodium quinoa seeds: taking fresh harvested chenopodium quinoa willd, and peeling chenopodium quinoa willd seeds;
(2) and (3) drying: screening quinoa grains, putting the screened quinoa grains in a tray, and drying in an oven;
(3) crushing and sieving: crushing chenopodium quinoa seeds in a flour mill, wherein the flour mill cannot be operated for a long time, so that the heating temperature of the flour mill is prevented from being too high to influence the later-stage extraction effect, sieving the chenopodium quinoa seed powder with a 60-mesh sieve, collecting undersize products, sealing the undersize products in a wide-mouth bottle, and storing the sealed chenopodium quinoa seeds in a cool and dry place for later use;
(4) adding ethanol to adjust the feed-liquid ratio: taking 10 g of quinoa wheat seed powder, and mixing with 75% ethanol solution according to a material-liquid ratio of 1: 20;
(5) ultrasonic treatment: treating the mixed solution of quinoa wheat seed powder and 75% ethanol in ultrasonic wave at 60 deg.C under 400W power for 60 min;
(6) centrifuging: centrifuging the mixed solution after ultrasonic treatment at 2800r/min for 5min, and collecting the supernatant;
(7) membrane filtration: filtering the supernatant with organic microporous filter membrane with pore diameter of 0.22 μm to obtain filtrate, i.e. rhizoma et radix Veratri Saponici extract;
(8) and (4) light-resistant cold storage: storing the crude extract of quinoa saponin in a refrigerator at-22 deg.C in dark for use.
According to the conventional determination method of the plant saponin, the content of the quinoa saponin is 82%, and the extraction rate is 8.8%.
Example 2
The extraction method of the quinoa saponins is characterized by comprising the following steps:
(1) harvesting chenopodium quinoa seeds: taking fresh harvested chenopodium quinoa willd, and peeling chenopodium quinoa willd seeds;
(2) and (3) drying: screening quinoa grains, putting the screened quinoa grains in a tray, and drying in an oven;
(3) crushing and sieving: crushing chenopodium quinoa seeds in a flour mill, wherein the flour mill cannot be operated for a long time, so that the heating temperature of the flour mill is prevented from being too high to influence the later-stage extraction effect, sieving the chenopodium quinoa seed powder with a 60-mesh sieve, collecting undersize products, sealing the undersize products in a wide-mouth bottle, and storing the sealed chenopodium quinoa seeds in a cool and dry place for later use;
(4) adding ethanol to adjust the feed-liquid ratio: taking 10 g of quinoa wheat seed powder, and mixing with 75% ethanol solution according to the material-liquid ratio of 1: 22;
(5) ultrasonic treatment: treating the mixed solution of quinoa wheat seed powder and 75% ethanol in ultrasonic wave at 55 deg.C under 420W power for 58 min;
(6) centrifuging: centrifuging the mixed solution after ultrasonic treatment at 2900r/min for 4min, and collecting supernatant;
(7) membrane filtration: filtering the supernatant with organic microporous filter membrane with pore diameter of 0.22 μm to obtain filtrate, i.e. rhizoma et radix Veratri Saponici extract;
(8) and (4) light-resistant cold storage: storing the crude extract of quinoa saponin in a refrigerator at-22 deg.C in dark for use.
According to the conventional determination method of the plant saponin, the content of the quinoa saponin is 80%, and the extraction rate is 8.2%.
Example 3
The extraction method of the quinoa saponins is characterized by comprising the following steps:
(1) harvesting chenopodium quinoa seeds: taking fresh harvested chenopodium quinoa willd, and peeling chenopodium quinoa willd seeds;
(2) and (3) drying: screening quinoa grains, putting the screened quinoa grains in a tray, and drying in an oven;
(3) crushing and sieving: crushing chenopodium quinoa seeds in a flour mill, wherein the flour mill cannot be operated for a long time, so that the heating temperature of the flour mill is prevented from being too high to influence the later-stage extraction effect, sieving the chenopodium quinoa seed powder with a 60-mesh sieve, collecting undersize products, sealing the undersize products in a wide-mouth bottle, and storing the sealed chenopodium quinoa seeds in a cool and dry place for later use;
(4) adding ethanol to adjust the feed-liquid ratio: taking 10 g of quinoa wheat seed powder, and mixing with 75% ethanol solution according to the material-liquid ratio of 1: 25;
(5) ultrasonic treatment: treating the mixed solution of quinoa wheat seed powder and 75% ethanol in ultrasonic wave at 50 deg.C under 450W power for 50 min;
(6) centrifuging: centrifuging the mixed solution after ultrasonic treatment at 3000r/min for 3min, and collecting supernatant;
(7) membrane filtration: filtering the supernatant with organic microporous filter membrane with pore diameter of 0.22 μm to obtain filtrate, i.e. rhizoma et radix Veratri Saponici extract;
(8) and (4) light-resistant cold storage: storing the crude extract of quinoa saponin in a refrigerator at-22 deg.C in dark for use.
According to the conventional determination method of the plant saponin, the content of the quinoa saponin is 75%, and the extraction rate is 6.5%.
Example 4
The extraction method of the quinoa saponins is characterized by comprising the following steps:
(1) harvesting chenopodium quinoa seeds: taking fresh harvested chenopodium quinoa willd, and peeling chenopodium quinoa willd seeds;
(2) and (3) drying: screening quinoa grains, putting the screened quinoa grains in a tray, and drying in an oven;
(3) crushing and sieving: crushing chenopodium quinoa seeds in a flour mill, wherein the flour mill cannot be operated for a long time, so that the heating temperature of the flour mill is prevented from being too high to influence the later-stage extraction effect, sieving the chenopodium quinoa seed powder with a 60-mesh sieve, collecting undersize products, sealing the undersize products in a wide-mouth bottle, and storing the sealed chenopodium quinoa seeds in a cool and dry place for later use;
(4) adding ethanol to adjust the feed-liquid ratio: taking 10 g of quinoa wheat seed powder, and mixing with 75% ethanol solution according to the material-liquid ratio of 1: 15;
(5) ultrasonic treatment: treating the mixed solution of quinoa wheat seed powder and 75% ethanol in ultrasonic wave at 70 deg.C under 350W power for 70 min;
(6) centrifuging: centrifuging the mixed solution after ultrasonic treatment at 2600r/min for 8min, and collecting supernatant;
(7) membrane filtration: filtering the supernatant with organic microporous filter membrane with pore diameter of 0.22 μm to obtain filtrate, i.e. rhizoma et radix Veratri Saponici extract;
(8) and (4) light-resistant cold storage: storing the crude extract of quinoa saponin in a refrigerator at-22 deg.C in dark for use.
The content of the quinoa saponin is 76 percent and the extraction rate is 7.5 percent according to the test of a conventional determination method of the plant saponin.
Comparative example 1
The difference from example 1 is that the temperature of the ultrasonic treatment of comparative example 1 was 15 deg.C, and other parameters and procedures were the same as those of example 1.
According to the conventional determination method of the plant saponin, the content of the quinoa saponin is 44%, and the extraction rate is 3.6%.
Comparative example 2
The difference from example 1 is that in step (4), the material-to-liquid ratio of quinoa wheat seed powder and 75% ethanol solution in comparative example 2 is 1:29, and other parameters and steps are the same as those in example 1.
According to the conventional determination method of the plant saponin, the content of the quinoa saponin is 59 percent, and the extraction rate is 5.6 percent.
Experimental example 1 evaluation of Natural moisturizing factor increasing Effect
In order to determine the expression promoting effect of the natural moisturizing factor of the quinoa saponin extracted by the present invention, the following experiment was performed.
Keratinocytes were cultured for a period of time in a keratinocyte growth medium containing a human keratinocyte growth additive, and 3 passages of the cells were used. After culturing 3 subcultured keratinocytes in a 6-well plate to 60-80%, each well was distinguished into a non-treated group, a group treated with quinoa saponin of example 1, and a group treated with quinoa saponin of example 1 plus 0.05% by mass of calcium, manganese, magnesium and zinc mineral components, and then cultured for 2 days. Then, the expression result of the filaggrin genetic gene and the expression condition of the loricrin genetic gene are determined by a conventional method.
The results showed that the filaggrin-promoting ability to promote skin moisture retention and the loricrin-promoting ability to promote skin barrier enhancement were the most excellent in the group treated with the quinoa saponin of example 1 and the group treated with the quinoa saponin of example 1 plus 0.05% by mass of mineral components such as calcium, manganese, magnesium and zinc, and that the group treated with only the quinoa saponin of example 1 exhibited the second best effect in the untreated group.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (10)
1. The extraction method of the quinoa saponins is characterized by comprising the following steps:
(1) harvesting chenopodium quinoa seeds: taking fresh harvested chenopodium quinoa willd, and peeling chenopodium quinoa willd seeds;
(2) and (3) drying: screening quinoa grains, and drying;
(3) crushing and sieving: crushing chenopodium quinoa seeds, sieving the chenopodium quinoa seeds with a 60-mesh sieve, collecting undersize, sealing, and storing in a cool and dry place for later use;
(4) adding ethanol and mixing: mixing quinoa seed powder with ethanol solution;
(5) ultrasonic treatment: carrying out ultrasonic treatment on the mixed solution of the quinoa wheat seed powder and ethanol;
(6) centrifuging: centrifuging the mixed solution after ultrasonic treatment, and taking supernatant;
(7) membrane filtration: filtering the supernatant with organic microporous membrane to obtain filtrate as rhizoma et radix Veratri Sativi saponin extractive solution;
(8) and (4) light-resistant cold storage: freezing the crude extract of quinoa saponin at low temperature and storing in dark place for later use.
2. The method for extracting quinoa saponin according to claim 1, wherein the volume concentration of the ethanol solution is 75%.
3. The method for extracting quinoa saponin according to claim 1, wherein the ratio of the mass of quinoa seed powder to the volume of the ethanol solution is 1: 15-25.
4. The method for extracting quinoa saponin as claimed in claim 1, wherein the ultrasonic power is 350-450W.
5. The method for extracting quinoa saponin according to claim 1, wherein the ultrasound time is 50-70 min.
6. The method for extracting quinoa saponin according to claim 1, wherein the ultrasonic temperature is 50-70 ℃.
7. The method for extracting quinoa saponin as claimed in claim 1, wherein the rotation speed of the centrifugation is 2600-3000 r/min.
8. The method for extracting quinoa saponin according to claim 1, wherein the centrifugation treatment time is 3-8 min.
9. The method for extracting quinoa saponin according to claim 1, wherein the pore size of the organic microporous filter membrane is 0.22 μm.
10. The use of quinoa saponin extracted by the method of any one of quinoa saponins of claims 1-9 in cosmetic skin care products.
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| CN113563408A (en) * | 2021-07-29 | 2021-10-29 | 上海理工大学 | Method for preparing saponin by utilizing chenopodium quinoa and separation and identification method of chenopodium quinoa saponin |
| CN114767595A (en) * | 2022-05-13 | 2022-07-22 | 山东福瑞达生物股份有限公司 | Preparation method and application of soothing and repairing composition |
| CN115531264A (en) * | 2022-09-23 | 2022-12-30 | 广东瑞驰生物科技有限公司 | Oil-control moisturizing exfoliating skin-cleaning product and preparation method thereof |
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| CN115531264A (en) * | 2022-09-23 | 2022-12-30 | 广东瑞驰生物科技有限公司 | Oil-control moisturizing exfoliating skin-cleaning product and preparation method thereof |
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Application publication date: 20200317 |