KR20110004993A - The cultivating method of functional mushroom calcium reinforced - Google Patents

Abstract

PURPOSE: A cultivation method of functional mushroom with the enhanced calcium content is provided to obtain functional food for the growth acceleration and the prevention of osteoporosis. CONSTITUTION: A cultivation method of functional mushroom with the enhanced calcium content comprises the following steps; mixing 30parts of fermented sawdust by weight, 30parts of poplar by weight, 2.8parts of beet pulp by weight, 4parts of cotton seed meal by weight, and other materials; adding 8 kinds of calcium to the obtained base material; mixing the mixture with distilled water to make a culture medium; making a culture media bottle and a culture media tube with the medium; cooling the bottle and the tube by 15deg C; injecting sawdust inoculums into the cooled culture media bottle and tube; and cultivating mycelia, and growing mushrooms.

Description

The cultivating method of functional mushroom calcium reinforced}

The present invention relates to a method for culturing functional mushrooms rich in calcium content, and compositions thereof.

Functional food development has been continuously increasing since the 1990s, and mushrooms have also been shown to have an adult disease and anticancer effect, and the demand for health foods is increasing day by day. Such mushrooms grow naturally and grow naturally in the natural environment, and in the case of native grow, there is a limit to the production, artificial cultivation is widely used, and cultivated in China, Japan and Korea for medicinal or edible use.

Mushrooms are unique in taste and aroma, and contain proteins, vitamins, and minerals. In minerals, potassium (K), copper (Cu), and iron (Fe) contents are generally high. Mn), molybdenum (Mo), zinc (Zn), calcium (Ca) and the like contain a small amount.

Calcium is one of the essential inorganic salts in the human body. Animals are mainly contained in bones, teeth, etc. in combination with phosphoric acid, and are also involved in physiology. It is enough for a person to consume about 0.8g a day. In Europe and the United States, the main sources of calcium are milk and dairy products, which are enough to consume about 1/3 to 1/2 of the above amounts.

However, in Korea, small bones and green leaves of fish are the main sources of supply, so the daily intake is about 0.5g, which requires more calcium intake. When ingested, it helps to increase the growth of the body and prevents osteoporosis. Especially during pregnancy and lactation (授乳 期) the need for more calcium.

Recently, various technologies have been developed to meet the above requirements.

For example, Korean Patent No. 0498724 relates to a mushroom cultivation method of increasing calcium content, artificially cultivating mushrooms and administering a medium in which water is diluted with water-soluble calcium to form a medium, sterilizing the copper medium, and mushrooms. Inoculating and cultivating spawn seedlings to cultivate mushrooms, after inoculating the medium for 20 days after inoculation of the seed culture, it is composed of a method of cultivating the mushrooms by removing the old inoculation bacteria and diluted water-soluble calcium to the medium.

However, the present invention, unlike the administration of water diluted with water-soluble calcium in the prior art is mixed together in the base material to make the medium, cultivated by varying the concentration of eight kinds of calcium and optimally contained in the optimal calcium content and mushrooms The experiment was conducted to find the type of calcium to be used, and the optimal conditions to apply to Pleurotus eryngii were limited to specific and accurate data, providing a method of cultivating optimal functional mushrooms at a low cost, and providing significant methods in terms of prior art, composition, and effect. There is one difference.

The present invention aims to develop a mushroom cultivation method for enhancing calcium to improve the problems that mushroom cultivation farmers face, and to enable the cultivation of new mushrooms with added functionality easily and conveniently for anyone.

In addition, the present invention is to add a low-cost calcium material to the mushroom medium to promote calcium content, promote the growth and development of children in the growing age, and to prevent the osteoporosis of the elderly menopausal as a functional food that can recognize the consumer to contribute to the promotion of mushroom consumption calcium The main challenge is to provide a method of cultivating functional mushrooms with enhanced ingredients.

In order to solve the above problems, the present invention is 30 parts by weight of untransported fermented sawdust, poplar 30 parts by weight, beet pulp 2.8 parts by weight, cottonseed foil 4 parts by weight, 0.2 parts by weight of crushed stone 0.2 parts by weight, corn cove 7.8 Mixing the base material at a ratio of 25 parts by weight to 25 parts by weight of corn flour and dividing the concentration of calcium to 1% to 5% in the base material, and mixing the base material and calcium powder in a dry state. And then adding distilled water to make a medium so that the moisture content is 60%, making the bottle containing the moisture absorbent medium in a dedicated bottle, and making a test tube medium by putting the absorbed medium in a dedicated test tube. The sterilized bottle and tube were cooled to 15 ° C. in a sterile chamber and 20 cc to 25 cc each of the sawdust inoculator, which had been previously incubated in a clean bench. Method of cultivating calcium-enhanced functional mushrooms comprising the steps of inoculating bacteria, culturing the mycelia on the inoculated bottle and in vitro medium and generating mushrooms on the bottle and in vitro medium after the incubation It can be solved by mushrooms grown by.

The present invention can add a low-cost calcium material to the mushroom medium to promote calcium content, promote the growth and development of growing children, and provide a functional food that can prevent osteoporosis in the elderly menopausal age can contribute to improving farm income and mushroom consumption The effect is expected.

In order to solve the above problems, the present invention will be described in more detail with reference to the following drawings and examples.

1 is a flow chart for the present inventors mushroom production.

Unripe fermented sawdust 30 parts by weight, poplar 30 parts by weight, beet pulp 2.8 parts by weight, cottonseed foil 4 parts by weight, 0.2 parts by weight of crushed stone, 0.2 parts by weight of dry paper, corn cob 7.8 parts by weight, corn flour 25 parts by weight Mix it. Here, the medium is prepared by adding 1% to 5% of the calcium powder as an additive material at a concentration of 1% to 5%.

Types of added calcium include tympanic membrane, eggshell, lactic acid, seaweed, milk, diphosphoric acid, carbonate, and CMC calcium. The basic material and calcium powder were sufficiently mixed in a dry state, and then adjusted to around 60% of water using distilled water. After mixing the medium so that the water is sufficiently infiltrated, the bottle medium is bottled at 800g and autoclaved at 121 ° C and 1.2 atm for 60 to 120 minutes. The test tube medium is bottled at 30g and autoclaved at 121 ° C and 1.2 atm for 90 minutes. After sterilization is complete, cool to 15 ℃ in a sterile chamber and inoculate 20-25cc each of the previously inoculated sawdust inoculation sources and incubate mycelia at 25 ± 2 ℃ and 70%.

After the culture is completed, 12 ± 2 ° C. and 95% of the conditions are maintained for 12 days, and the condition of 2000 lux roughness allows the mushrooms to develop and germinate.

≪ Example 1 >

In order to analyze the calcium content, the mushroom fruiting body generated by each calcium treatment group took the carbonized sample in a painting vessel for carbon analysis, and heated it at a temperature of 550 ~ 600 ℃ for several hours until white-gray ash was obtained. Conversation. After cooling the ash, carefully wet it with 1N hydrochloric acid solution, add about 10 ml of hydrochloric acid solution (1 → 2), and evaporate to dryness in a water bath. Then, about 8 to 10 ml of hydrochloric acid solution (1 → 4) was added to the dried product, and the resultant was heated and filtered through a 100 ml volumetric flask. The insoluble matter is transferred to the painting container used as it was, dried, and then painted again. Wet the ash with water, add about 2 ml of hydrochloric acid solution (1 → 4), dilute it with about 5 ml of 1N hydrochloric acid solution, and warm it in a water bath. Fill the filtered solution with a 100 ml volumetric flask and add 1N hydrochloric acid solution. This solution is used as the test solution. To the 1N hydrochloric acid solution, lanthanum chloride (LaCl ₃ ) is added to 1000 ppm as La, or strontium chloride (SrCl ₂ ) is added to 5000 ppm as Sr. As described above, the measurement is performed using equipment widely used as an analytical device such as a hot block system for acid digestion, PerkinElmer ICP-OES, calibration mechanical pipettes and tips, and a water bath.

Figure 2 is a graph measuring the calcium content of the fruiting body for each type of calcium medium of the present invention by the above method.

As a result of analyzing the calcium content of the mushrooms generated in the bottle cultivation test by each treatment group, the untreated group was 33mg per 100g of fresh mushroom, whereas the tympanic calcium treated group showed the highest calcium content of 79mg. The calcium lactate and calcium carbonate treatment groups, which were the cheapest treatment weight per kg of calcium used in the present invention, were 45 mg and 50 mg, and the lowest calcium content among the treatment groups was investigated. It was found that 46.5mg of calcium per 100g of shiitake mushroom was treated with 2% added eggshell calcium.

Example 2

Figure 3 is a graph comparing the mushroom production according to the type of calcium addition of the present invention.

According to the present invention calcium cultivated functional mushroom cultivation method 12 days after the development treatment 800g when the inoculation of mushrooms by calcium treatment concentration of 234g in the untreated group, while the second calcium phosphate treated 188g, calcium lactate treated 180g, tympanic calcium treated 174g The production rate was lower than that of the non-treated area. However, the present invention results in a mushroom yield of 92 ± 12g at 49% oak sawdust + corncob 21% + rice bran 12% + wheat bran 12% + 6% oxyphyll content after mixing 850g of water content of 66% of the medium. The yield of mushrooms in all treatments was higher than in the study of artificial cultivation of large elk, Korean Journal of Mycology, 2003).

<Example 3>

Figure 4 is a graph comparing the mushroom generation recovery and weight loss rate by calcium treatment of the present invention. The recovery rate of mushrooms is closely related to the weight reduction rate. As the weight loss rate increases, the media degradation index of the mycelia increases, indicating the amount of feeding of the medium. As a result of analyzing the mushroom incidence recovery and calcium content of the mushrooms prepared according to the present invention by calcium treatment medium, the calcium of the mushroom produced in the dicalcium phosphate treatment with high recovery rate of 24% was 44 mg per 100 g of fresh mushroom, rather than 33 mg of the untreated group. The cornea treated with tympanic membrane, which was highly investigated and had a relatively high recovery rate of 22%, showed the highest calcium content of 79 mg per 100 g of fresh mushroom. In addition, all of the calcium-treated treatments used in the present invention showed a recovery rate of about 20% and a weight loss rate of about 30%, indicating that the higher the weight loss rate, the more the mycelial degradation index reached a certain level. .

<Example 4>

Figure 5 is a graph of the physical properties of the mushrooms generated in the calcium-treated medium of the present invention.

Physical properties are used to measure the freshness of mushrooms. The physical properties of mushrooms are closely related to hardness, and the higher hardness affects the maintenance of freshness of fruiting bodies, so the cultivation method of increasing hardness will increase the shelf life. That's how you can do it. Referring to FIG. 5, when the physical properties of the non-treated group were 100, the milk calcium treated group was 171g and the calcium lactate treated group was 159g, which was relatively high. Egg shell calcium treated group showed a lower hardness than the non-treated group 97mg.

As a result, the mushrooms cultivated through the cultivation method of the present invention, in addition to the advantages of containing calcium, it can be seen that the commercial property is superior to the general untreated mushrooms.

<Example 5>

Figure 6 is a graph of the pH change of the medium for calcium addition type of the present invention. The medium acidity of the dicalcium phosphate treatment group, which was excellent in mushroom production in the cultivation method of the present invention at 188 g, was investigated at pH 5.4 after mushroom generation, and in the case of the calcium lactate treatment group exhibiting 180 g production, the acidity of the medium after mushroom generation was pH 5.8. In the case of tympanic calcium treated with 174 g of production, the pH of the medium after mushroom development was investigated to pH 5.7.

1 is a flow chart of the inventors mushroom manufacturing

Figure 2 is a graph of calcium content of the fruiting body of each type of calcium medium of the present invention

Figure 3 is a graph of mushroom production according to the type of calcium addition of the present invention

Figure 4 is a graph of mushroom recovery rate and weight reduction rate of the calcium treatment according to the present invention

5 is a graph of the physical properties of the mushrooms generated in the calcium-treated medium of the present invention

6 is a graph showing the pH change of the medium for calcium addition type of the present invention

Claims (4)

Unripe fermented sawdust 30 parts by weight, poplar 30 parts by weight, beet pulp 2.8 parts by weight, cottonseed foil 4 parts by weight, 0.2 parts by weight of crushed stone, 0.2 parts by weight of dry paper, corn cob 7.8 parts by weight, corn flour 25 parts by weight Mixing the; Making a mixed medium by dividing the concentration of calcium to 1% to 5% in the base material; Mixing the base material and calcium powder in a dry state and adding distilled water to make a medium such that the moisture content is 60%; Making a bottle medium by putting the medium having absorbed the water into a dedicated bottle; Making a tube incorporating the moisture absorbent medium into a dedicated test tube; Cooling the sterilized bottle medium and the test tube medium to 15 ° C. in a clean room; Inoculating the cooled bottle medium and the test tube medium with 20cc to 25cc of the sawdust inoculator previously incubated in a clean bench; Culturing the mycelia on the inoculated bottle and in vitro medium; Generating mushrooms in the bottle medium and the test tube medium after the incubation; Functional mushroom cultivation method of calcium enhanced. The method of claim 1, The calcium is a calcium-enhanced functional composition comprising the steps of mixing the eight types of calcium, tympanic calcium, eggshell calcium, calcium lactate, algae calcium, milk calcium, dicalcium phosphate, calcium carbonate, CMC calcium into the base material How to grow mushrooms. The method of claim 1, The step of making the bottle medium is autoclaving for 60 to 120 minutes at 121 ℃, 1.2 atm; The step of making the test tube medium is autoclaving for 90 minutes at 121 ℃, 1.2 atm; Culturing the mycelium of the bottle and the test tube medium is maintained at 25 ± 2 ℃, 70% condition; The step of generating the mushrooms after the culture of the hyphae 12 ± 2 ℃, 95% condition for 12 days, the step of maintaining the conditions of roughness 2000lux, calcium-enhanced functional mushroom cultivation method characterized in that it is added. A calcium-enhanced functional mushroom, which is cultivated by the cultivation method of any one of claims 1 to 3.

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