KR101083143B1 - The cultivating method of functional mushroom calcium reinforced - Google Patents
The cultivating method of functional mushroom calcium reinforced Download PDFInfo
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- KR101083143B1 KR101083143B1 KR1020090062456A KR20090062456A KR101083143B1 KR 101083143 B1 KR101083143 B1 KR 101083143B1 KR 1020090062456 A KR1020090062456 A KR 1020090062456A KR 20090062456 A KR20090062456 A KR 20090062456A KR 101083143 B1 KR101083143 B1 KR 101083143B1
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- 239000011575 calcium Substances 0.000 title claims abstract description 78
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 title claims abstract description 77
- 229910052791 calcium Inorganic materials 0.000 title claims abstract description 77
- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 62
- 238000000034 method Methods 0.000 title claims abstract description 10
- 238000012360 testing method Methods 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000000463 material Substances 0.000 claims abstract description 12
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 8
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 8
- 235000005822 corn Nutrition 0.000 claims abstract description 8
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 6
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 claims abstract description 6
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 5
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 5
- 239000001506 calcium phosphate Substances 0.000 claims abstract description 5
- 210000003278 egg shell Anatomy 0.000 claims abstract description 5
- 235000013336 milk Nutrition 0.000 claims abstract description 5
- 239000008267 milk Substances 0.000 claims abstract description 5
- 210000004080 milk Anatomy 0.000 claims abstract description 5
- 235000016068 Berberis vulgaris Nutrition 0.000 claims abstract description 4
- 241000335053 Beta vulgaris Species 0.000 claims abstract description 4
- 235000019739 Dicalciumphosphate Nutrition 0.000 claims abstract description 4
- 241000219000 Populus Species 0.000 claims abstract description 4
- 238000001816 cooling Methods 0.000 claims abstract description 4
- 235000012343 cottonseed oil Nutrition 0.000 claims abstract description 4
- NEFBYIFKOOEVPA-UHFFFAOYSA-K dicalcium phosphate Chemical compound [Ca+2].[Ca+2].[O-]P([O-])([O-])=O NEFBYIFKOOEVPA-UHFFFAOYSA-K 0.000 claims abstract description 4
- 229910000390 dicalcium phosphate Inorganic materials 0.000 claims abstract description 4
- 229940038472 dicalcium phosphate Drugs 0.000 claims abstract description 4
- 239000012153 distilled water Substances 0.000 claims abstract description 4
- 235000013312 flour Nutrition 0.000 claims abstract description 4
- 239000011888 foil Substances 0.000 claims abstract description 4
- 239000004575 stone Substances 0.000 claims abstract description 4
- 229910000019 calcium carbonate Inorganic materials 0.000 claims abstract description 3
- GBCAVSYHPPARHX-UHFFFAOYSA-M n'-cyclohexyl-n-[2-(4-methylmorpholin-4-ium-4-yl)ethyl]methanediimine;4-methylbenzenesulfonate Chemical compound CC1=CC=C(S([O-])(=O)=O)C=C1.C1CCCCC1N=C=NCC[N+]1(C)CCOCC1 GBCAVSYHPPARHX-UHFFFAOYSA-M 0.000 claims abstract description 3
- 241000209149 Zea Species 0.000 claims abstract 4
- 241000195493 Cryptophyta Species 0.000 claims abstract 2
- 238000012364 cultivation method Methods 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 4
- 230000002745 absorbent Effects 0.000 claims 1
- 239000002250 absorbent Substances 0.000 claims 1
- 238000000338 in vitro Methods 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 229960005069 calcium Drugs 0.000 abstract description 68
- 238000011161 development Methods 0.000 abstract description 6
- 239000001527 calcium lactate Substances 0.000 abstract description 5
- 229960002401 calcium lactate Drugs 0.000 abstract description 5
- 235000011086 calcium lactate Nutrition 0.000 abstract description 5
- 208000001132 Osteoporosis Diseases 0.000 abstract description 4
- 235000013376 functional food Nutrition 0.000 abstract description 4
- 239000000843 powder Substances 0.000 abstract description 4
- 229960003563 calcium carbonate Drugs 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 238000011282 treatment Methods 0.000 description 15
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 14
- 239000000243 solution Substances 0.000 description 10
- 238000004519 manufacturing process Methods 0.000 description 9
- 238000011084 recovery Methods 0.000 description 7
- 238000011081 inoculation Methods 0.000 description 5
- 230000000704 physical effect Effects 0.000 description 5
- 240000008042 Zea mays Species 0.000 description 4
- 230000004580 weight loss Effects 0.000 description 4
- 239000010949 copper Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 238000010422 painting Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 210000003454 tympanic membrane Anatomy 0.000 description 2
- 239000013585 weight reducing agent Substances 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241001474374 Blennius Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 240000000599 Lentinula edodes Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- 101100496858 Mus musculus Colec12 gene Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 244000252132 Pleurotus eryngii Species 0.000 description 1
- 235000001681 Pleurotus eryngii Nutrition 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 238000000184 acid digestion Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 210000004087 cornea Anatomy 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-N diphosphoric acid Chemical compound OP(O)(=O)OP(O)(O)=O XPPKVPWEQAFLFU-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000002354 inductively-coupled plasma atomic emission spectroscopy Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000006651 lactation Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- ICAKDTKJOYSXGC-UHFFFAOYSA-K lanthanum(iii) chloride Chemical compound Cl[La](Cl)Cl ICAKDTKJOYSXGC-UHFFFAOYSA-K 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 239000011733 molybdenum Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 229910001631 strontium chloride Inorganic materials 0.000 description 1
- AHBGXTDRMVNFER-UHFFFAOYSA-L strontium dichloride Chemical compound [Cl-].[Cl-].[Sr+2] AHBGXTDRMVNFER-UHFFFAOYSA-L 0.000 description 1
- JBQYATWDVHIOAR-UHFFFAOYSA-N tellanylidenegermanium Chemical compound [Te]=[Ge] JBQYATWDVHIOAR-UHFFFAOYSA-N 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
- A01G18/22—Apparatus for the preparation of culture media, e.g. bottling devices
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/50—Inoculation of spawn
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/306—Foods, ingredients or supplements having a functional effect on health having an effect on bone mass, e.g. osteoporosis prevention
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Abstract
본 발명은 칼슘이 강화된 기능성 버섯의 재배방법에 관한 것으로서, 더욱 상세하게는 미송발효톱밥 30중량부, 포플러 30중량부, 비트펄프 2.8중량부, 면실박 4중량부, 패화석 0.2중량부, 건비지 0.2중량부, 콘코브 7.8중량부, 옥수수가루 25중량부의 비율로 기본재료를 혼합하는 단계와; 고막칼슘, 난각칼슘, 젖산칼슘, 해조칼슘, 밀크칼슘, 제 2인산칼슘, 탄산칼슘, CMC칼슘을 상기 기본재료에 중량비 기준1%~5% 까지 농도를 나누어 혼합배지를 만드는 단계와; 상기 기본재료와 칼슘분말을 건조상태에서 혼합한 후 증류수를 넣어 수분함량이 60중량%가 되도록 배지를 만드는 단계와; 상기 수분을 흡수한 배지를 병에 800g 넣어 121℃, 1.2기압에서 60~120분간 고압살균하여 병배지를 만드는 단계와; 상기 수분을 흡수한 배지를 시험관에 30g 넣어 121℃, 1.2기압에서 90분간 고압살균하여 시험관 배지를 만드는 단계와; 상기 병배지와 시험관배지를 무균실에서 15℃로 냉각시키는 단계와; 상기 냉각된 병배지와 시험관배지를 클린벤치에서 미리 배양해둔 톱밥접종원을 20cc~25cc씩 균을 접종하는 단계와; 상기 접종된 병배지와 시험관배지를 25±2℃, 70% 조건으로 균사를 배양하는 단계; 로 구성된다.The present invention relates to a method of cultivating calcium-enhanced functional mushrooms, more specifically, 30 parts by weight of untransferred sawdust, poplar 30 parts by weight, beet pulp 2.8 parts, cottonseed foil 4 parts by weight, crushed stone 0.2 parts by weight, gun Mixing the base material at a ratio of 0.2 part by weight of busy weight, 7.8 parts by weight of corn cob, and 25 parts by weight of corn flour; Making a mixed medium by dividing calcium, eggshell calcium, calcium lactate, algae calcium, milk calcium, dicalcium phosphate, calcium carbonate and CMC calcium in the base material by weight ratio of 1% to 5% by weight; Mixing the base material and the calcium powder in a dry state and adding distilled water to make a medium such that the moisture content is 60% by weight; Putting the water-absorbing medium into the bottle 800g to autoclave at 121 ° C. and 1.2 atm for 60 to 120 minutes to make a bottle medium; Putting 30 g of the medium having absorbed the water into a test tube to autoclave at 121 ° C. and 1.2 atm for 90 minutes to make a test tube medium; Cooling the bottle medium and the test tube medium to 15 ° C. in a sterile chamber; Inoculating the cooled bottle medium and the test tube medium with 20cc to 25cc of the sawdust inoculator previously incubated in a clean bench; Cultivating the mycelia at 25 ± 2 ° C., 70% of the inoculated bottle and test tube medium; It consists of.
본 발명은 쉽고 간편하게 칼슘 함량이 풍부한 기능성 버섯의 재배가 가능하도록 하여 농가 소득증대에 이바지 할 수 있다. 또한 성장기 어린이의 성장발육을 촉진하고 갱년기 노령층의 골다공증을 예방할 수 있는 기능성 식품으로 소비자를 재인식시켜 버섯의 소비촉진에 기여할 수 있다.The present invention can be easily and simply cultivated calcium-rich functional mushrooms can contribute to the increase of farm income. It can also contribute to promoting mushroom consumption by recognizing consumers as functional foods that can promote the growth and development of growing children and prevent osteoporosis of elderly men.
기능성, 버섯, 칼슘, Functional, mushroom, calcium,
Description
본 발명은 칼슘 함량이 풍부한 기능성 버섯의 재배방법 및 그 조성물에 관한 것이다.The present invention relates to a method for culturing functional mushrooms rich in calcium content, and compositions thereof.
기능성 식품개발이 1990년대 이후 지속적으로 증가 추세에 있으며, 버섯 또한 성인병과 항암효과가 있음이 입증되면서 건강식품으로 그 수요가 날로 증가되고 있다. 이와 같은 버섯은 자연환경에서 자생하는 것과 인공적으로 재배되는 것이 있으며 자생의 경우에는 생산량에 한계가 있고 인공재배가 널리 이용되고 있으며 중국, 일본 및 우리나라에서 약용 또는 식용으로 널리 재배되고 있다. Functional food development has been continuously increasing since the 1990s, and mushrooms have also been shown to have an adult disease and anticancer effect, and the demand for health foods is increasing day by day. Such mushrooms grow naturally and grow naturally in the natural environment, and in the case of native grow, there is a limit to the production, artificial cultivation is widely used, and cultivated in China, Japan and Korea for medicinal or edible use.
버섯은 종류에 따라 맛과 향이 독특하고 단백질, 비타민, 무기물을 함유하고 있으며, 무기물에는 칼륨(K), 구리(Cu), 철(Fe)의 함량은 대체적으로 높게 나타나고 있으나 인체의 효소기능인 망간(Mn), 몰리브덴(Mo), 아연(Zn), 칼슘(Ca)등은 미량을 함유하고 있다.Mushrooms are unique in taste and aroma, and contain proteins, vitamins, and minerals. In minerals, potassium (K), copper (Cu), and iron (Fe) contents are generally high. Mn), molybdenum (Mo), zinc (Zn), calcium (Ca) and the like contain a small amount.
칼슘은 인체에 꼭 필요한 무기염류 중 하나로, 동물체에는 주로 인산과 결합하여 뼈·이[齒] 등에 함유되어 있으며, 그밖에 생리작용에도 관여한다. 사람은 하 루에 0.8g 정도 섭취하면 충분하다. 유럽·미국 등에서는 칼슘의 주공급원이 우유나 유제품이며, 이것만으로도 위에서 말한 양의 1/3~1/2 정도가 보급될 정도로 칼슘의 섭취가 풍부하다.Calcium is one of the essential inorganic salts in the human body. Animals are mainly contained in bones, teeth, etc. in combination with phosphoric acid, and are also involved in physiology. It is enough for a person to consume about 0.8g a day. In Europe and the United States, the main sources of calcium are milk and dairy products, which are enough to consume about 1/3 to 1/2 of the above amounts.
그러나 한국의 경우는 생선의 작은 뼈나 녹색 나뭇잎 등을 주요 보급원으로 하고 있기 때문에 하루의 섭취량은 0.5g 정도에 머물고 있어 보다 충분한 칼슘의 섭취가 필요하다. 섭취시에 신체의 발육증진에 도움을 주고, 골다공증등을 예방한다. 특히 임신했을 때나 수유기(授乳期)에는 칼슘의 필요가 더욱 요구된다.However, in Korea, small bones and green leaves of fish are the main sources of supply, so the daily intake is about 0.5g, which requires more calcium intake. When ingested, it helps to increase the growth of the body and prevents osteoporosis. Especially during pregnancy and lactation (授乳 期) the need for more calcium.
최근 상기의 요구를 부응하기 위해서 여러기술이 개발되고 있다.Recently, various technologies have been developed to meet the above requirements.
예를 들어, 국내등록특허 제0498724호는 칼슘함량이 증강되는 버섯재배방법에 관한 것으로서, 버섯을 인공 재배하여 배지에 수용성 칼슘을 희석시킨 용수를 투여하여 배지를 조성하고, 동 배지를 살균하고 버섯종균을 접종시키고 배양시켜 버섯을 재배함에 있어서 배지에 종균접종 후 20일 정도 배양시킨 후 노화된 접종균을 제거하고 수용성칼슘을 희석시킨 용수를 배지에 투여하여 버섯을 재배하는 방법으로 구성되어 있다.For example, Korean Patent No. 0498724 relates to a mushroom cultivation method of increasing calcium content, artificially cultivating mushrooms and administering a medium in which water is diluted with water-soluble calcium to form a medium, sterilizing the copper medium, and mushrooms. Inoculating and cultivating spawn seedlings to cultivate mushrooms, after inoculating the medium for 20 days after inoculation of the seed culture, it is composed of a method of cultivating the mushrooms by removing the old inoculation bacteria and diluted water-soluble calcium to the medium.
그러나, 본 발명은 선행기술이 수용성 칼슘을 희석시킨 용수를 투여한 것과 달리 배지를 만드는 기본재료에 함께 혼합하고, 8종의 칼슘의 농도를 달리하여 재배하고 최적의 칼슘함량과 버섯에 최적으로 함유되는 칼슘의 종류를 찾는 실험을 병행하였으며 새송이버섯에 적용하는 최적의 조건을 구체적이고 정확한 데이터로 한정하여 비용이 적게들며 최적의 기능성 버섯을 재배하는 방법을 제공하여 선행기술과 구성과 효과면에서 현격한 차이가 있다.However, the present invention, unlike the administration of water diluted with water-soluble calcium in the prior art is mixed together in the base material to make the medium, cultivated by varying the concentration of eight kinds of calcium and optimally contained in the optimal calcium content and mushrooms The experiment was conducted to find the type of calcium to be used, and the optimal conditions to apply to Pleurotus eryngii were limited to specific and accurate data, providing a method of cultivating optimal functional mushrooms at a low cost, and providing significant methods in terms of prior art, composition, and effect. There is one difference.
본 발명은 버섯재배 농가가 처해 있는 문제점을 개선하고 누구나 쉽고 편하게 기능성이 가미된 새로운 버섯의 재배가 가능하도록 칼슘을 강화시키는 버섯의 재배법을 개발하고자 한다. The present invention aims to develop a mushroom cultivation method for enhancing calcium to improve the problems that mushroom cultivation farmers face, and to enable the cultivation of new mushrooms with added functionality easily and conveniently for anyone.
또한 본 발명은 저가의 칼슘재료를 버섯배지에 첨가함으로써 칼슘함량을 증진시키고 성장기 어린이의 성장발육을 촉진하고 갱년기 노령층의 골다공증을 예방할 수 있는 기능성 식품으로 소비자를 재인식시켜 버섯의 소비촉진에 기여할 수 칼슘성분이 강화된 기능성 버섯의 재배방법을 제공하는 것을 해결하고자 하는 주된 과제로 삼는다.In addition, the present invention is to add a low-cost calcium material to the mushroom medium to promote calcium content, promote the growth and development of children in the growing age, and to prevent the osteoporosis of the elderly menopausal as a functional food that can recognize the consumer to contribute to the promotion of mushroom consumption calcium The main challenge is to provide a method of cultivating functional mushrooms with enhanced ingredients.
상기와 같은 과제를 해결하기 위해서, 본 발명은 미송발효톱밥 30중량부, 포플러 30중량부, 비트펄프 2.8중량부, 면실박 4중량부, 패화석 0.2중량부, 건비지 0.2중량부, 콘코브 7.8중량부, 옥수수가루 25중량부의 비율로 기본재료를 혼합하는 단계와 상기 기본재료에 칼슘을 중량비 기준으로 1%~5% 까지 농도를 나누어 혼합배지를 만드는 단계와 상기 기본재료와 칼슘분말을 건조상태에서 혼합한 후 증류수를 넣어 수분함량이 60중량%가 되도록 배지를 만드는 단계와 상기 수분을 흡수한 배지를 전용병에 넣어 병배지를 만드는 단계와 상기 수분을 흡수한 배지를 전용시험관에 넣어 시험관배지를 만드는 단계와 상기 병배지와 시험관배지를 무균실에서 15℃로 냉각시키는 단계와 상기 냉각된 병배지와 시험관배지를 클린벤치에서 미리 배양해둔 톱밥접종원을 20cc~25cc씩 균을 접종하는 단계와 상기 접종된 병배지와 시험관배지에 균사를 배양하는 단계와 상기 배양 후 병배지와 시험관배지에 버섯을 발생시키는 단계로 구성되는 칼슘이 강화된 기능성 버섯 재배방법 및 상기의 방법에 의해 재배되는 버섯에 의해서 해결할 수 있다.In order to solve the above problems, the present invention is 30 parts by weight of untransported fermented sawdust,
본 발명은 저가의 칼슘재료를 버섯배지에 첨가하여 칼슘함량을 증진시키고 성장기 어린이의 성장발육을 촉진하고 갱년기 노령층의 골다공증을 예방할 수 있는 기능성 식품을 제공하여 농가 소득향상과 버섯의 소비촉진에 기여할 수 있는 효과가 기대된다.The present invention can add a low-cost calcium material to the mushroom medium to promote calcium content, promote the growth and development of growing children, and provide a functional food that can prevent osteoporosis in the elderly menopausal age can contribute to improving farm income and mushroom consumption The effect is expected.
상기의 과제를 해결하기 위하여 이하의 도면과 실시예를 통하여 본 발명을 더욱 상세하게 설명한다.In order to solve the above problems, the present invention will be described in more detail with reference to the following drawings and examples.
도 1은 본 발명인 버섯제조를 위한 순서도이다.1 is a flow chart for the present inventors mushroom production.
미송발효톱밥 30중량부, 포플러 30중량부, 비트펄프 2.8중량부, 면실박 4중량부, 패화석 0.2중량부, 건비지 0.2중량부, 콘코브 7.8중량부, 옥수수가루 25중량부의 비율로 기본재료를 혼합한다. 여기에 첨가재료로 칼슘분말 8종에 대해 중량비 기준 1%~5%의 농도별로 첨가하여 배지를 제조한다.Unripe fermented
첨가칼슘의 종류는 고막, 난각, 젖산, 해조, 밀크, 제2인산, 탄산, CMC칼슘을 사용한다. 기본재료와 칼슘분말은 건조상태에서 충분히 혼합시킨 후 증류수를 이용하여 수분을 60중량% 내외로 조절하였다. 수분이 충분히 배어들도록 배지를 혼합한 다음 병배지는 800g씩 입병하고 121℃, 1.2기압에서 60~120분 고압살균하고 시험관배지는 30g씩 입병하여 121℃, 1.2기압에서 90분간 고압살균한다. 살균이 완료되면 무균실에서 15℃로 냉각시키고 미리 배양해둔 톱밥접종원을 20~25cc씩 균을 접종한 후 25±2℃, 70% 조건으로 균사를 배양한다.Types of added calcium include tympanic membrane, eggshell, lactic acid, seaweed, milk, diphosphoric acid, carbonate, and CMC calcium. The base material and calcium powder were sufficiently mixed in a dry state, and then adjusted to about 60% by weight of water using distilled water. After mixing the medium so that the water is sufficiently infiltrated, the bottle medium is bottled at 800g and autoclaved at 121 ° C and 1.2 atm for 60 to 120 minutes. The test tube medium is bottled at 30g and autoclaved at 121 ° C and 1.2 atm for 90 minutes. After sterilization is complete, cool to 15 ℃ in a sterile chamber and inoculate 20-25cc each of the previously inoculated sawdust inoculation sources and incubate mycelia at 25 ± 2 ℃ and 70%.
배양이 완료된 배지는 12±2℃, 95% 조건으로 12일간, 조도 2000lux의 조건을 유지하여 버섯이 발생하여 발아되도록 한다.After the culture is completed, 12 ± 2 ° C. and 95% of the conditions are maintained for 12 days, and the condition of 2000 lux roughness allows the mushrooms to develop and germinate.
<실시예 1>≪ Example 1 >
칼슘함량을 분석하기 위해서 칼슘처리구별로 발생된 버섯 자실체이 칼슘함량을 분석하기 위해 검체를 회화용기에 취하여 탄화시킨 후 550~600℃의 온도에서 여러시간 가열하여 백색~회백색의 회분이 얻어질 때까지 회화한다. 상기의 회분을 방냉 후 주의하여 1N염산용액으로 적신 후 염산용액(1→2) 약 10㎖를 가해 수욕상에서 완전 증발건고시킨다. 상기의 건고물에 다시 염산용액(1→4) 약 8~10㎖를 가해 수분 가열 후 100㎖ 메스플라스크에 여과한다. 불용물은 여지와 같이 사용했던 회화용기에 옮겨 건고한 후 다시 회화한다. 상기 회분을 물로 적시어 염산용액(1→4)약 2㎖를 가해 1N염산용액 약5㎖로 희석한 후 수욕상에서 가온하고, 여과한 액을 앞의 100㎖ 메스플라스크에 채워 1N염산용액을 가하여 100㎖로 하여 시험용액으로 한다. 상기 1N염산용액에는 La으로서 1000ppm이 되도록 염화란탄(LaCl3)을 첨가하거 나 또는 Sr으로서 5000ppm이 되도록 염화스트론티움(SrCl2)을 첨가하여 사용한다. 상기와 같은 방법으로 hot block system for acid digestion, PerkinElmer ICP-OES, Calibration mechanical pipettes and tips, Water bath와 같은 분석장비로 널리 사용되고 있는 장비를 이용하여 측정한다.In order to analyze the calcium content, the mushroom fruiting body generated by each calcium treatment group took the carbonized sample in a painting vessel for carbon analysis, and heated it at a temperature of 550 ~ 600 ℃ for several hours until white-gray ash was obtained. Conversation. After cooling the ash, carefully wet it with 1N hydrochloric acid solution, add about 10 ml of hydrochloric acid solution (1 → 2), and evaporate to dryness in a water bath. Then, about 8 to 10 ml of hydrochloric acid solution (1 → 4) was added to the dried product, and the resultant was heated and filtered through a 100 ml volumetric flask. The insoluble matter is transferred to the painting container used as it was, dried, and then painted again. Wet the ash with water, add about 2 ml of hydrochloric acid solution (1 → 4), dilute it with about 5 ml of 1N hydrochloric acid solution, and warm it in a water bath. Fill the filtered solution with a 100 ml volumetric flask and add 1N hydrochloric acid solution. This solution is used as the test solution. To the 1N hydrochloric acid solution, lanthanum chloride (LaCl 3 ) is added to 1000 ppm as La, or strontium chloride (SrCl 2 ) is added to 5000 ppm as Sr. As described above, the measurement is performed using equipment widely used as an analytical device such as a hot block system for acid digestion, PerkinElmer ICP-OES, calibration mechanical pipettes and tips, and a water bath.
도 2는 상기의 방법으로 본 발명의 칼슘배지 종류별 자실체의 칼슘함량 측정한 그래프이다.Figure 2 is a graph measuring the calcium content of the fruiting body for each type of calcium medium of the present invention by the above method.
각 처리구별 병재배시험에서 발생된 버섯의 칼슘함량을 분석한 결과 무처리구가 생버섯 100g당 33mg인 반면 고막칼슘처리구가 79mg으로 가장 높은 칼슘함량을 보인 것으로 나타났다. 본 발명에 사용한 칼슘중 kg당 가장 저렴했던 처리구인 젖산칼슘과 탄산칼슘 처리구는 45mg과 50mg으로 처리구중 가장 칼슘함량이 낮은 것으로 조사되었다. 이는 기존의 난각칼슘이 2% 첨가된 처리구가 표고버섯 100g당 46.5mg이 칼슘함량이 조사되었다As a result of analyzing the calcium content of the mushrooms generated in the bottle cultivation test by each treatment group, the untreated group was 33mg per 100g of fresh mushroom, whereas the tympanic calcium treated group showed the highest calcium content of 79mg. The calcium lactate and calcium carbonate treatment groups, which were the cheapest treatment weight per kg of calcium used in the present invention, were 45 mg and 50 mg, and the lowest calcium content among the treatment groups was investigated. It was found that 46.5mg of calcium per 100g of shiitake mushroom was treated with 2% added eggshell calcium.
<실시예2>Example 2
도 3은 본 발명의 칼슘첨가 종류별 버섯생산량 비교 그래프이다.Figure 3 is a graph comparing the mushroom production according to the type of calcium addition of the present invention.
본 발명인 칼슘이 강화된 기능성 버섯 재배방법에 따라 발생처리 12일후 800g 입병시 칼슘처리 농도별 버섯발생량은 무처리구가 234g인 반면 제2인산칼슘처리구는 188g, 젖산칼슘처리구는 180g, 고막칼슘처리구는 174g으로 비교적 무처리구보다 저조한 생산량을 보였다. 그러나 본 발명은 참나무톱밥 49%+콘코브 21%+미강 12%+ 밀기울 12%+ 옥피분6%를 배합후 850g 입병한 배지의 함수율 66%일때 버섯생산량이 92±12g이라는 결과(이대진등, 큰느타리의 인공재배에 관한 연구,한국균학회지, 2003)보다는 모든 처리구의 버섯생산량이 높게 나타났다.According to the present invention calcium cultivated functional mushroom cultivation method 12 days after the development treatment 800g when the inoculation of mushrooms by calcium treatment concentration of 234g in the untreated group, while the second calcium phosphate treated 188g, calcium lactate treated 180g, tympanic calcium treated 174g The production rate was lower than that of the non-treated area. However, the present invention results in a mushroom yield of 92 ± 12g at 49% oak sawdust +
<실시예 3><Example 3>
도 4는 본 발명의 칼슘 처리구별 버섯발생 회수율 및 중량감소율 비교 그래프이다. 버섯발생 회수율은 중량감소율과 밀접한 관계를 가지는데 중량 감소율이 많을수록 균사의 배지 분해지수가 높아 배지의 양양이 공급되는 정도를 알 수 있다. 본 발명에 따라 제조한 버섯의 칼슘처리 배지별 버섯발생 회수율과 칼슘함량을 분석한 결과로서 회수율이 24%로 높은 제2인산 칼슘처리구에서 발생된 버섯의 칼슘은 생버섯 100g당 44mg으로 무처리구의 33mg보다는 높게 조사되었고 비교적 회수율이 22%로 높았던 고막칼슘처리구가 생버섯 100g당 79mg으로 가장 높은 칼슘함량을 보였다. 그외에도 본 발명에 이용되는 칼슘이 처리된 처리구들 모두가 20%안팎의 회수율과 30%안팎의 중량감소율을 보여주어서 중량감소율이 많을수록 보여주는 균사의 배지 분해지수가 일정수준에 도달하고 있음을 알수있다.Figure 4 is a graph comparing the mushroom generation recovery and weight loss rate by calcium treatment of the present invention. The recovery rate of mushrooms is closely related to the weight reduction rate. As the weight loss rate increases, the media degradation index of the mycelia increases, indicating the amount of feeding of the medium. As a result of analyzing the mushroom incidence recovery and calcium content of the mushrooms prepared according to the present invention by calcium treatment medium, the calcium of the mushroom produced in the dicalcium phosphate treatment with high recovery rate of 24% was 44 mg per 100 g of fresh mushroom, rather than 33 mg of the untreated group. The cornea treated with tympanic membrane, which was highly investigated and had a relatively high recovery rate of 22%, showed the highest calcium content of 79 mg per 100 g of fresh mushroom. In addition, all of the calcium-treated treatments used in the present invention showed a recovery rate of about 20% and a weight loss rate of about 30%, indicating that the higher the weight loss rate, the more the mycelial degradation index reached a certain level. .
<실시예 4><Example 4>
도 5는 본 발명의 칼슘 처리배지에서 발생된 버섯의 물성검사 그래프이다.Figure 5 is a graph of the physical properties of the mushrooms generated in the calcium-treated medium of the present invention.
물성은 버섯의 신선도를 측정할 수 있는 척도로 사용되는 데 버섯의 물성은 경도와 밀접한 연관이 있는 것으로 경도가 높을수록 자실체의 신선도를 유지하는데 영향을 주므로 경도를 높여주는 재배법은 곧 유통기간을 증대시킬 수 있는 방법이다. 도 5를 보면 무처리구의 물성을 100이라하면 밀크칼슘 처리구가 171g, 젖산칼슘 처리구는 159g으로 비교적 높게 조사되었다. 난각칼슘처리구는 무처리구보다 낮은 97mg을 나타내어 낮은 경도를 보였다.Physical properties are used to measure the freshness of mushrooms. The physical properties of mushrooms are closely related to hardness, and the higher hardness affects the maintenance of freshness of fruiting bodies, so the cultivation method of increasing hardness will increase the shelf life. That's how you can do it. Referring to FIG. 5, when the physical properties of the non-treated group were 100, the milk calcium treated group was 171g and the calcium lactate treated group was 159g, which was relatively high. Egg shell calcium treated group showed a lower hardness than the non-treated group 97mg.
이로써 본 발명의 재배방법을 통해 재배된 버섯은 칼슘을 함유하는 장점외에 일반적인 무처리 버섯보다 상품성도 우수함을 알 수 있다.As a result, the mushrooms cultivated through the cultivation method of the present invention, in addition to the advantages of containing calcium, it can be seen that the commercial property is superior to the general untreated mushrooms.
<실시예 5>Example 5
도 6은 본 발명의 칼슘첨가 종류별 배지의 pH 변화 그래프이다. 본 발명의 재배방법 중 버섯생산량이 188g으로 우수했던 제2인산칼슘처리구의 배지산도는 버섯발생 후 pH 5.4로 조사되었고, 180g 생산량을 보인 젖산칼슘처리구의 경우 버섯발생 후 배지의 산도가 pH 5.8을 나타냈고 174g의 생산량을 보인 고막칼슘처리구의 경우 버섯발생 후 배지산도가 pH 5.7로 조사되었다. Figure 6 is a graph of the pH change of the medium for calcium addition type of the present invention. The medium acidity of the dicalcium phosphate treatment group, which was excellent in mushroom production in the cultivation method of the present invention at 188 g, was investigated at pH 5.4 after mushroom generation, and in the case of the calcium lactate treatment group exhibiting 180 g production, the acidity of the medium after mushroom generation was pH 5.8. In the case of tympanic calcium treated with 174 g of production, the pH of the medium after mushroom development was investigated to pH 5.7.
도 1은 본 발명인 버섯제조순서도1 is a flow chart of the inventors mushroom manufacturing
도 2는 본 발명의 칼슘배지 종류별 자실체의 칼슘함량 그래프Figure 2 is a graph of calcium content of the fruiting body of each type of calcium medium of the present invention
도 3은 본 발명의 칼슘첨가 종류별 버섯생산량 비교 그래프Figure 3 is a graph of mushroom production according to the type of calcium addition of the present invention
도 4는 본 발명의 칼슘 처리구별 버섯발생 회수율 및 중량감소율 비교 그래프Figure 4 is a graph of mushroom recovery rate and weight reduction rate of the calcium treatment according to the present invention
도 5는 본 발명의 칼슘 처리배지에서 발생된 버섯의 물성검사 그래프5 is a graph of the physical properties of the mushrooms generated in the calcium-treated medium of the present invention
도 6은 본 발명의 칼슘첨가 종류별 배지의 pH 변화 그래프6 is a graph showing the pH change of the medium for calcium addition type of the present invention
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