KR20100111066A - Skin whitening composition - Google Patents

Abstract

PURPOSE: A composition for skin whitening with an effect of suppressing melanogenesis is provided. CONSTITUTION: A composition for skin whitening contains seaweed extract or a portion of the seaweed extract as an active ingredient. The seaweed extract is isolated using methanol, distilled water, ethanol, acetone, ethyl acetate, saturated noram butanol, chloroform, methylene chloride, water, or a mixture solvent thereof. The seaweed includes Petalonia binghamiae, Sargassum nipponicum Yendo, Dictyopteris divaricata, and Myelophycus simplex. The portion is obtained by extracting with water, distilled water, ethanol, or a mixture solvent, removing extraction solvent, and fractioning into ethanol layer and, ethyl acetate layer. The composition is used in the form of an external use composition or soap composition.

Description

Skin Whitening Composition

The present invention relates to a composition for skin whitening, and relates to a composition for skin whitening comprising as an active ingredient, seaweed iron extract, small cap extract, rock extract.

It is well known that skin whitening is directly related to melanin pigment.

Melanin is made by tyrosine (tyrosine) is oxidized by tyrosinase (tyrosine) to dopa (DOPA, 3,4-dihydroxyphenylalanine), dopa is oxidized back to dopaquinone (dopaquinone), and then made through a non-enzymatic oxidation reaction.

Melanin protects sub-dermal skin organs by blocking UV rays, and protects the damage of proteins and genes in the skin caused by free radicals generated in the skin. It causes hyperpigmentation such as blemishes and freckles.

Therefore, inhibiting the production of melanin can bring the effect of skin whitening.

The effects of skin whitening by inhibiting melanin production include para-methoxyphenol, hydroquinone, kojic acid, arbutin, vitamin C (ascorbic acid), Compounds such as glutathione and cysteine, and plant extracts such as lettuce extract, licorice extract, peony extract, cinnamon extract, brown root extract, Angelica extract, bark extract, halved extract, aloe extract and the like are known.

The present invention is also conceived in that the algae extract has a melanin production inhibitory effect.

The present invention is to provide a composition for skin whitening.

Specific embodiments of the present invention will be presented below.

The present inventors, as confirmed in the following Examples and Experimental Examples, seaweeds of the seaweed, the little hat, slippery net horse, etc. 29 kinds of seaweeds of <Table 1> below extracted with 80% ethanol and the extract is distilled water and ethyl After fractionation with acetate layer, the ethyl acetate layer fractions were treated with B16F10, a melanoma cell, except for azaleas, thin canes, ecstasy, wrinkled red leaves, sesame gambling, wrinkled soybean fields, cephalopods, and black skin. And all the rest of the algae extract was found to have a clear melanin inhibitory effect.

The present invention is provided based on the above experimental results.

Therefore, the composition having a skin whitening effect of the present invention (hereinafter, "skin whitening composition") has no effect of inhibiting melanin production in algae of <Table 1>, buttock hair, thin dog gambling, ecstasy, wrinkled red leaves, gambling, Seaweeds except Petalonia binghamiae , Sargassum nipponicum Yendo, Dictyopteris divaricata, Myelophycus simplex, Loosearia hakodatens ), Dictyopteris prolifera, Prionitis cornea, L ( Ishige okamurae Yendo), Grain grass ( Bonnemaisonia hamifera), Scorching ( Laciacia intricata Lamouroux), Scytosiphon lomentaria jinu Ari (Grateloupia filicina), bulre end (Colpomenia sinuosa), rock patter (Leathesia difformis), part chaetmal (Padina arborescens), netting basket (final hole bolre) (Hydroclathrus clathratus), the twin- Chamber (Laurensia okamurae), seosil (Chondria crassicaulis), the strata (Sargassum thunbergii), thin seaweed chamber (Scytosiphon gracilis) and Baby lychee ( Gloiopeltis furcata) is characterized in that it comprises an algae extract of any one of the active ingredient.

In the present specification, "algae extract" refers to an extract obtained by extracting the algae from methanol, distilled water, ethanol, acetone, ethyl acetate, saturated normal butanol, chloroform, methylene chloride, water, or a mixed solvent thereof regardless of the extraction method. It is understood as meaning including the fraction further purified with the solvents listed above in the extract. Regardless of the extraction method, as long as the seaweeds to be extracted are extracted by immersing them in the extraction solvent, it should be understood that the extraction method can be applied to any method such as cooling, refluxing, heating, and ultrasonic wave. Nevertheless, the "seaweed extract" is preferably extracted with seaweed, distilled water, ethanol or a mixed solvent thereof to remove the extraction solvent to obtain a solid extract, and the solid extract is fractionated into an ethanol layer and an ethyl acetate layer. Refers to the ethyl acetate layer fraction obtained after.

In addition, in the present specification, "skin whitening" is understood as a result of the inhibition of the production of melanin, specifically the symptoms resulting from the increase of melanin as the production of melanin is inhibited (the composition for skin whitening of the present invention is pharmaceutical When understood as a composition such symptoms may be regarded as a disease) are understood as a result of being prevented or inhibited.

Examples of such symptoms include blemishes, freckles, and skin aging as described above, but as shown in the following Examples and Experimental Examples of the present invention, algae extracts included in the skin whitening composition of the present invention inhibit melanin production. As long as it is obvious to show the effect of the above, the above symptom is a symptom resulting from an increase in melanin and should be understood as all symptoms leading to the result of skin whitening by preventing or inhibiting it.

In addition to the seaweed extract as an active ingredient, the composition for skin whitening of the present invention may include a compound or plant extract as previously exemplified, which is known to have a skin whitening effect so as to enhance or enhance the skin whitening effect, and also skin whitening. It may include other known compounds or plant extracts known to be effective, and may further include compounds or plant extracts having a skin whitening effect that will be revealed in the future.

Herein, the other compounds or plant extracts include mercaptosuccinic acid, mercaptodextran, tefrenone, dihydroxy-isoquinoline, indomethacin, 3-hydroxymanul, vitamin K, thiazolidone, kynurenine, and lemon. Extract, cucumber extract, mulberry extract, licorice extract, rosemary extract, acerola cherry extract, ginkgo extract, carob (carob) extract, geranium extract, herbal extract, and the like, but is not limited thereto.

Compound or plant extract as exemplified above may be included in the composition for skin whitening of the present invention together with one or more seaweed extract.

On the other hand, in the composition for skin whitening of the present invention, the seaweed extract as an active ingredient may be included in any amount within a range that does not have toxicity to the human body depending on the purpose, formulation (cosmetic, soap, ointment, etc.). For example, the composition may be included in an amount of 0.05 wt% to 99.99 wt% with respect to the total weight of the composition.

The skin whitening composition of the present invention can be regarded as a cosmetic composition in a specific embodiment.

When the composition for skin whitening of the present invention is understood as a cosmetic composition, the cosmetic composition can be prepared in various forms, for example, emulsion, lotion, cream (oil-in-water type, water-in-oil type, multiphase), solution, suspension (Anhydrous and aqueous), anhydrous products (oil and glycols), gels, masks, packs or powders and the like.

The cosmetic composition for skin whitening of the present invention may include an acceptable carrier in cosmetic preparations in addition to seaweed extract.

Herein, "acceptable carriers in cosmetic preparations" are compounds or compositions already known and used that may be included in cosmetic preparations, or compounds or compositions which will be developed in the future, which have no toxicity, instability or irritation more than the body can adapt to in contact with the skin. Say that.

The carrier may be included in the cosmetic composition for skin whitening of the present invention at about 1% by weight to about 99.99% by weight, preferably about 50% to about 99% by weight of the composition.

However, since the ratio depends on the form of the above-described preparation of the cosmetic of the present invention and its specific application site (face or hand) or its preferred application amount, the ratio is in any aspect the scope of the present invention. It should not be understood as limiting.

Meanwhile, examples of the carrier include alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosity modifiers, emulsions, stabilizers, sunscreen agents, colorants, fragrances, and the like.

Alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, humectants, viscosity modifiers, emulsions, stabilizers, sunscreens, colorants, compounds / compositions that can be used as fragrances, etc., which can be used as the carrier are already known in the art. As a result, those skilled in the art can select and use appropriate materials / compositions.

On the other hand, the skin lightening composition of the present invention can be regarded as a pharmaceutical composition in another specific embodiment.

When the composition for skin whitening of the present invention is identified as a pharmaceutical composition, the pharmacological effect may be understood as a treatment or prevention effect for a disease resulting from an increase in melanin pigment, and the skin whitening is for the treatment or prevention of the disease. It can be understood as a result of.

Here, the disease resulting from the increase of the melanin pigment is understood to include diseases which will be revealed in the future as well as diseases which have been discovered so far for the aforementioned reasons.

Nevertheless, the disease resulting from the increase of the melanin pigment preferably means a disease that has been discovered to date, and more preferably, a disease as exemplified above, among other known diseases, that is, blemishes, freckles, and skin aging.

The pharmaceutical compositions of the invention may be administered orally or parenterally during clinical administration, but will typically be administered directly to the skin topically in an external formulation.

In addition, the pharmaceutical composition of the present invention may be formulated using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents and surfactants that are commonly used. Solid preparations for oral administration include tablets, pills, powders, granules, and capsules, and such solid preparations contain at least one excipient such as starch, calcium carbonate, sucrose, lactose and gelatin in the seaweed extract. Mix is prepared. Lubricants may also be used in addition to simple excipients. Liquid preparations for oral administration include suspensions, solvents, emulsions, and syrups.In addition to the commonly used simple diluents, water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives, etc. May be included. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized formulations and suppositories. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used. As the base of the suppository, Whitepsol, macrogol, tween 61, cacao butter, laurin butter, glycerol, gelatin and the like can be used.

The daily dosage of the pharmaceutical composition of the present invention is usually 0.001 ~ 150 mg / kg body weight range, it can be administered once or divided into several times. However, since the actual dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as the route of administration, the age, sex, weight of the patient, and the severity of the patient, the dosage is in any aspect the scope of the present invention. It should not be understood as limiting.

The skin whitening composition of the present invention may be regarded as a soap composition in another specific embodiment.

When the composition for skin whitening of the present invention is understood as a soap composition, the soap composition of the present invention may be prepared by including a seaweed extract on a soap base, and may be prepared by including a skin moisturizer, an emulsifier, a hardener, and the like as an additive. Can be.

As the soap base material, vegetable oils such as palm oil, palm oil, soybean oil, perm oil, olive oil, palm kernels, or animal oils such as tallow, pork, sunny and fish oils can be used, and the skin moisturizing agent is glycerin, erythritol and polyethylene glycol. , Propylene glycol, butylene glycol, pentylene glycol, hexyl glycol, isopropyl myristate, silicone derivatives, aloe vera, sorbitol and the like can be used, the emulsifiers such as natural oils, wax fatty alcohols, hydrocarbons, natural plant extracts, etc. May be used, and tetrasodium idieti may be used as the water softener.

The soap composition of the present invention may further include an antimicrobial agent, a dispersant, a foam inhibitor, a solvent, a scale inhibitor, a corrosion inhibitor, a perfume, a pigment, a metal ion blocking agent, an antioxidant, a preservative, and the like as an additive.

In the soap composition of the present invention, the soap base or additives may be included in an amount generally used in the art, and the soap base is generally 99.9 to 50 wt% based on the total content of the soap composition. The additive may be added in an amount of 1 wt% to 20 wt%.

Here, the content of the seaweed extract in the soap composition is effective as described above in the composition for skin whitening of the present invention.

As described above, according to the present invention, a composition for skin whitening is provided.

The composition of the present invention may exhibit a skin whitening effect by including an algae extract having an inhibitory effect on melanin production.

According to a specific aspect of the present invention, there is provided a cosmetic composition, a pharmaceutical composition, a food composition and a soap composition for skin whitening.

Hereinafter, the present invention will be described with reference to Examples. However, the scope of the present invention is not limited to these examples.

Example 1 Preparation of Extract of Seaweed

<Table 1> 29 kinds of seaweeds were immersed in 80% ethanol at room temperature After extracting for 24 hours, the extraction residue was removed and concentrated under reduced pressure to remove the extraction solvent to obtain a solid extract. 3 g of the solid extract of each seaweed was added to 300 ml of distilled water and 300 ml of ethyl acetate, and extracted at room temperature for 72 hours. The ethyl acetate layer was concentrated under reduced pressure and lyophilized. Each sample was aliquoted and dissolved in DMSO.

Seaweed Used for Extraction Sample Number sample Scientific name JBR163 Seaweed Petalonia binghamiae JBR243 Little hat Sargassum nipponicum Yendo JBR246 Slippery Net Dictyopteris divaricata JBR256 Rockbeard Myelophycus simplex JBR261 Baby Lomentaria hakodatensis Yendo JBR267 Barbed bone horse Dictyopteris prolifera JBR268 Red black pepper Prionitis cornea JBR270 Jindubal Chondrus ocellatus Holmes JBR271 tile Ishige okamurae Yendo JBR272 Thin dog gambling Grateloupia lanceolata JBR274 Owl Bonnemaisonia hamifera JBR276 Skein Laurencia intricata Lamouroux JBR278 Ecklonia Ecklonia cava Kjellman JBR345 Ringhawk Scytosiphon lomentaria JBR348 Empty Charms Grateloupia filicina JBR351 End of fire Colpomenia sinuosa JBR354 Wrinkled red leaves Callophyllis crispata JBR359 Rock Leathesia difformis JBR368 Buchat Padina arborescens JBR374 Net Basket (hole bolder end) Hydroclathrus clathratus JBR375 Twin room Laurensia okamurae JBR376 Room Chondria crassicaulis JBR510 Gambling Grateloupia elliptica JBR511 Worm Sargassum thunbergii JBR512 Thin Seaweed Room Scytosiphon gracilis JBR514 Wrinkled hair Chondrus crispus JBR518 Baby Pool Starfish Gloiopeltis furcata JBR519 Black meat Carpopeltis affinis JBR538 톳 Hizikia fusiformis

Example 2 Cell Culture

Mouse melanoma cells, B16F10 cells are penicillin-streptomycin 100 units / mL and 10% fetal bovine serum (FBS) containing a DMEM medium 37 ℃ using (GIBCO, Grand Island, NY, USA), 5% CO 2 thermostat Cultured at, and subcultured once every 4 days.

Example 3 Cytotoxicity Evaluation

Cytotoxicity was evaluated by MTT assay to determine the effect of EtOAc fraction of algae on the viability of B16F10 cells. B16F10 cells (2 × 10 ⁴ cells / ml) were inoculated in a 96 well plate, and 18 hours before incubation, followed by treatment with 29 extracts of the algae extracted with EtOAc at 37 ° C., 10% CO ₂ cell incubator for 3 days. Incubated at. 200 μl of the MTT solution prepared at a concentration of 2 mg / ml PBS was added thereto, followed by incubation for 4 hours under the same culture conditions. The culture was removed and absorbance was measured at 570 nm by adding 200 µl of DMSO per well.

The results are shown in FIG. 1. Referring to Figure 1, it does not show the overall cytotoxicity, but the horned grass (JBR274), Korimae (JBR345), empty sesame root (JBR348), wrinkled red leaves (JBR354), net basket (JBR374) The back shows some cytotoxicity.

Example 4 Measurement of Melanin Synthesis Inhibition Rate

B16F10 cells were adjusted to 1 × 10 ⁵ cells / mL using DMEM medium added with 10% FBS, and then inoculated into 6 well plates, and cultured 18 hours before. Samples (29 extracts of the algae extracted using EtOAc) And melanin-forming stimulant α-MSH (50 nM) was incubated for 3 days. After 3 days, the plate medium was removed and washed twice with PBS. The washed cells were harvested, and the cells were completely dissolved by adding 1 N NaOH, and measured by ELISA at 405 nm.

The results are shown in FIG. 2. Jindubal (JBR272), Thin dog gambling (JBR272), Ecklonia cauliflower (JBR278), Wrinkled red leaf (JBR354), Cham gambling (JBR510), Wrinkled soybean field (JBR514), Aegean grass (JBR518), and Blacktail (JBR519) Except for the distinct melanin production inhibitory effect. In the case of sorghum grass (JBR274), Korimae (JBR345), empty sesame seeds (JBR348), and net basket (JBR374), the cell survival rate of less than 80% is shown when referring to FIG. When the degree is suppressed to the control group (negative control group, untreated group of α-MSH), it can be seen that there is an inhibitory effect on melanogenesis.

1 is a cytotoxicity evaluation results by MTT assay of seaweed extract.

Figure 2 is a result showing the melanin production inhibitory effect of seaweed extract.

Claims (5)

Petalonia binghamiae , Sargassum nipponicum Yendo, Dytyopteris divaricata, Myelophycus simplex, Lomentaria hakodatensis Yendo, Dytyopteris red prolifera Prionitis cornea, l ( Ishige okamurae Yendo), scallop ( Bonnemaisonia hamifera), corkscrew ( Laurecia intricata Lamouroux), Scytosiphon lomentaria, Grateloupia filicina , the end of the fire ( Colpomenia sinuosa) ), Leathesia difformis, Buchat horse ( Padina arborescens), Basket ( Holeclathrus clathratus), Twins ( Larensia okamurae), Chondria crassicaulis, Sargassum thunbergii Seaweed Room ( Scytosiphon gracilis) and Seaweed extract obtained by extracting any seaweed selected from the group consisting of Gloiopeltis furcata with methanol, distilled water, ethanol, acetone, ethyl acetate, saturated normal butanol, chloroform, methylene chloride, water, or a mixed solvent thereof Skin whitening composition comprising the fraction as an active ingredient. The method of claim 1, The fraction is an ethyl acetate layer fraction obtained by extracting with water, distilled water, ethanol or a mixed solvent thereof, removing the extraction solvent to obtain a solid extract, and fractionating the solid extract into an ethanol layer and an ethyl acetate layer. Skin whitening composition, characterized in that. The method according to claim 1 or 2, The composition is a composition for skin whitening, characterized in that the cosmetic composition. The method according to claim 1 or 2, The composition is a pharmaceutical composition, the pharmaceutical composition has a pharmacological effect of preventing or treating blemishes, freckles or skin aging, a disease resulting from an increase in melanin, the formulation thereof is a skin external composition Whitening composition. The method according to claim 1 or 2, The composition is a composition for skin whitening, characterized in that the soap composition.

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