CN108210375B - A facial mask containing herba Nepetae Divaricati extract and its preparation method - Google Patents
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Abstract
The invention discloses a mask containing a herba gynostemmatis segeti extract, which comprises the following components in percentage by mass: 0.05-1% of the kohlrabi extract, 0.5-1% of the polysaccharide of the agaric bifidus, 0.5-1% of the polysaccharide of the agaric glaucopiae, 0.2-1% of hyaluronic acid, 10-20% of glycerol and 78-88% of deionized water; the acanthus ilicifolius extract is an acanthus ilicifolius water extraction and alcohol precipitation supernatant part obtained by extracting acanthus ilicifolius through a water extraction and alcohol precipitation method, the agaricus bisporus polysaccharide is obtained by extracting acanthus bisporus powder, and the agaricus hybridus polysaccharide is obtained by extracting agaricus bisporus powder. The invention also discloses a preparation method of the facial mask containing the gynura segetum extract, and the prepared facial mask has whitening and antioxidant effects and meets the functional requirements of consumers on facial mask products.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to a mask containing a herba gynostemae extract and a preparation method thereof.
Background
The Chinese materia medica describes that the seaweed iron nail dish (with the scientific name of Ishige okamurae) has human edibility, is a traditional Chinese herbal medicine which can be taken orally, has the functions of softening hardness and resolving stagnation, removing toxicity and expelling ascaris, and can also be applied to cervical lymphadenectasis, goiter and laryngitis. The geographical location of the brown algae gynura bicolor has east Asia characteristics, and the research object of the invention is gynura bicolor produced in Quanzhou of Fujian province. The vegetables of the branchlet have been proved to have many biological activities by many studies, and are described as follows by way of example: cell experiment results show that a polyphenol compound contained in an ethyl acetate layer of a methanol extract of the herb of Ottelia has neuroprotective activity; oral administration of methanol extract of herba Nepetae Divaricati can improve hyperglycemia of type II diabetic mice.
According to data, no relevant research paper or product applying the acanthopanax herb on the facial mask is shown at present. According to the information known by the inventor, we should propose for the first time the concept of adding the herba gynandrae segeti itself or its extract into the facial mask, and design a preparation method of the facial mask containing the herba gynandrae segeti.
Disclosure of Invention
The invention aims to provide a facial mask containing a herba gynostemmatis segeti extract, which has whitening and antioxidant effects and meets the functional requirements of consumers on facial mask products.
In order to achieve the above purpose, the solution of the invention is:
a facial mask containing a herba gynostemae extract comprises the following components in percentage by mass:
the acanthus ilicifolius extract is an acanthus ilicifolius water extraction and alcohol precipitation supernatant part obtained by extracting acanthus ilicifolius through a water extraction and alcohol precipitation method, the agaricus bisporus polysaccharide is obtained by extracting acanthus bisporus powder, and the agaricus hybridus polysaccharide is obtained by extracting agaricus bisporus powder.
A facial mask containing a herba gynostemae extract comprises the following components in percentage by mass:
a method for preparing facial mask containing herba Nepetae Divaricati extract comprises the following steps:
(1) preparation of the kohlrabi extract:
① cleaning fresh herba Nerii, draining, and pulverizing into herba Nerii powder;
② mixing pulverized herba Nepetae Divaricati powder with distilled water at a ratio of 1g to 30mL, extracting with water bath at 90-100 deg.C for 1-2 hr twice, and filtering to obtain filtrate;
③ concentrating the filtrate under reduced pressure to 23-43% of the original volume of the filtrate, adding anhydrous ethanol to make the volume fraction of the anhydrous ethanol in the mixed system be 75%, precipitating with ethanol at 4 deg.C for 24h, centrifuging to obtain supernatant and ethanol precipitate;
④ concentrating the supernatant under reduced pressure, and vacuum freeze drying to obtain herba Nepetae Divaricati water extraction and ethanol precipitation supernatant;
(2) preparing polysaccharide of the double-split sea sedge: soaking the double-split sea sedge powder in 0.15mol/L hydrochloric acid solution, stirring, centrifuging, taking out a clear solution, adding 2-4 times of volume of absolute ethyl alcohol, stirring, precipitating, and performing vacuum freeze drying to obtain double-split sea sedge polysaccharide;
(3) preparing the tin blue sea agaric polysaccharide: soaking the tremella mesenterica powder in 0.15mol/L hydrochloric acid solution, stirring, centrifuging, taking out a clear solution, adding 2-4 times of anhydrous ethanol, stirring, precipitating, and performing vacuum freeze drying to obtain the polysaccharide of the tremella mesenterica;
(4) adding the polysaccharide of the agaric bifida obtained in the step (2), the polysaccharide of the agaric glaucopiae obtained in the step (3), hyaluronic acid, glycerol and deionized water into the supernatant part of the water extraction and alcohol precipitation of the acanthopanax japonicus obtained in the step (1) in sequence, wherein the mass ratio of the polysaccharide to the hyaluronic acid to the glycerol is that the supernatant part of the water extraction and alcohol precipitation of the acanthopanax japonicus: double split sea ear polysaccharide: polysaccharide of the fungus Stylosanthes guianensis: hyaluronic acid: glycerol: deionized water 0.05 to 1: 0.1-0.5: 0.1-0.5: 0.2-1: 10-20: 78-88, stirring, sealing and packaging to obtain the mask.
In the step (1), the reduced pressure concentration is carried out at 65 ℃ and-0.09 MPa, the rotation speed of the centrifugation is 4000r/min, the time of the centrifugation is 5min, and the temperature of the vacuum freeze drying is-80 ℃.
In the step (2), the stirring time for the first time is 72 hours, the stirring time for the second time is 24 hours, the rotation speed of the centrifugation is 4500r/min, the time of the centrifugation is 10 minutes, and the temperature of the vacuum freeze drying is-80 ℃.
In the step (3), the stirring time for the first time is 72 hours, the stirring time for the second time is 24 hours, the rotation speed of the centrifugation is 4500r/min, the time of the centrifugation is 10 minutes, and the temperature of the vacuum freeze drying is-80 ℃.
By adopting the technical scheme, the facial mask containing the acanthopanax senticosus extract disclosed by the invention is added with the acanthopanax senticosus extract, the agaricus bisporus polysaccharide and the agaricus hycus polysaccharide, and has the following beneficial effects:
(1) in vitro experiments prove that the ethanol extract of the gynura bicolor has antioxidant activity and has the capability of removing dibenzo-bitter acyl free radicals (2, 2-diphenyl-1-piperidinylhydrazyl (DPPH)), and the methanol extract of the gynura bicolor in the enclosed area of Fujian province also proves that the methanol extract of the gynura bicolor has higher content of polyphenol and antioxidant activity, wherein the main antioxidant active site is an ethyl acetate site. The Huangxiadong et al also confirmed that the supernatant of the water extraction and alcohol precipitation of the Oncorhynchus plant can inhibit the activity of tyrosinase (has whitening effect) and also has the effect of scavenging dibenzo bitter acyl radicals (i.e. antioxidant effect). In addition to whitening being a common product efficacy appeal for cosmetics, the addition of antioxidants to cosmetics has also become a new approach to skin care in recent years. Therefore, the herba gynostemae extract has two characteristics of whitening, antioxidation and the like, and endows the facial mask product with required efficacy appeal.
(2) The water extract of Tremella bifida can eliminate free radicals, and has antioxidant effect. In addition, the invention also selects the water extract of the large-scale algae of the different species of the same genus and the different species of the large-scale algae of the tinkalanchoe, and the water extract also has the antioxidation effect, and the prior researchers carry out component analysis on the extracts of the double-split auricularia or the tinkalanchoe, and the main active components are considered to be polysaccharide. Therefore, the agaric double-split polysaccharide and the agaric scholartree polysaccharide have antioxidation and also meet the efficacy requirements of the mask product.
Drawings
FIG. 1 is a schematic diagram showing the inhibitory effect of the supernatant of the water extraction and alcohol precipitation of Nemacystus decipiens on tyrosinase activity.
FIG. 2 is a schematic diagram showing the effect of the supernatant of the water extraction and alcohol precipitation of the Nemacystus decipiens on the removal of DPPH free radicals.
Detailed Description
In order to further explain the technical solution of the present invention, the present invention is explained in detail by the following specific examples.
Preparation of facial mask
Example 1
A facial mask containing herba Nepetae Divaricati extract (per 30 g) comprises the following components:
a method for preparing facial mask containing herba Nepetae Divaricati extract comprises the following steps:
(1) preparation of the kohlrabi extract:
① cleaning fresh herba Nerii, draining, and pulverizing into herba Nerii powder;
② mixing pulverized herba Nepetae Divaricati powder with distilled water at a ratio of 1g to 30mL, extracting with water bath at 90 deg.C for 2 hr twice, and filtering to obtain filtrate;
③ concentrating the filtrate under reduced pressure at 65 deg.C and-0.09 MPa to 30% of the original volume of the filtrate, adding anhydrous ethanol to make the volume fraction of the anhydrous ethanol in the mixed system 75%, precipitating with ethanol at 4 deg.C for 24h, and centrifuging at 4000r/min for 5min to obtain supernatant and ethanol precipitate;
④ at 65 deg.C and-0.09 MPa, vacuum concentrating the supernatant, and vacuum freeze drying at-80 deg.C to obtain herba Nepetae Divaricati water extract and ethanol precipitation supernatant;
(2) preparing polysaccharide of the double-split sea sedge: soaking the double-split sea sedge powder in 0.15mol/L hydrochloric acid solution, stirring for 72h, centrifuging at the rotating speed of 4500r/min for 10min, taking out the clear solution, adding 3 times of volume of absolute ethyl alcohol, stirring for 24h, precipitating, and vacuum drying at-80 ℃ to obtain double-split sea sedge polysaccharide;
(3) preparing the tin blue sea agaric polysaccharide: soaking the Hibiscus tin-orchid Merrill powder in 0.15mol/L hydrochloric acid solution, stirring for 72h, centrifuging at the rotating speed of 4500r/min for 10min, taking out the clear solution, adding 3 times of anhydrous ethanol, stirring for 24h, precipitating, and vacuum drying at-80 ℃ to obtain Hibiscus tin-orchid Merrill polysaccharide;
(4) and (2) adding 150mg of the polysaccharide of the agaricus bisporus obtained in the step (2), 150mg of the polysaccharide of the agaricus bisporus obtained in the step (3), 285mg of hyaluronic acid, 3g of glycerol and 26.4g of deionized water in sequence into 15mg of the supernatant obtained by water extraction and alcohol precipitation of the sowthistle herb in the step (1), stirring, sealing and packaging to obtain the mask containing the extract of the sowthistle herb.
Example 2
A facial mask containing herba Nepetae Divaricati extract (per 30 g) comprises the following components:
a method for preparing facial mask containing herba Nepetae Divaricati extract comprises the following steps:
(1) preparation of the kohlrabi extract:
① cleaning fresh herba Nerii, draining, and pulverizing into herba Nerii powder;
② mixing pulverized herba Nepetae Divaricati powder with distilled water at a ratio of 1g to 30mL, extracting with water bath at 100 deg.C for 2 hr twice, and vacuum filtering to obtain filtrate;
③ concentrating the filtrate under reduced pressure at 65 deg.C and-0.09 MPa to 33% of the original volume of the filtrate, adding anhydrous ethanol to make the volume fraction of the anhydrous ethanol in the mixed system 75%, precipitating with ethanol at 4 deg.C for 24h, and centrifuging at 4000r/min for 5min to obtain supernatant and ethanol precipitate;
④ at 65 deg.C and-0.09 MPa, vacuum concentrating the supernatant, and vacuum freeze drying at-80 deg.C to obtain herba Nepetae Divaricati water extract and ethanol precipitation supernatant;
(2) preparing polysaccharide of the double-split sea sedge: soaking the double-split sea sedge powder in 0.15mol/L hydrochloric acid solution, stirring for 72h, centrifuging at the rotating speed of 4500r/min for 10min, taking out the clear solution, adding 4 times of volume of absolute ethyl alcohol, stirring for 24h, precipitating, and vacuum drying at-80 ℃ to obtain double-split sea sedge polysaccharide;
(3) preparing the tin blue sea agaric polysaccharide: soaking the Hibiscus tin-orchid Merrill powder in 0.15mol/L hydrochloric acid solution, stirring for 72h, centrifuging at the rotating speed of 4500r/min for 10min, taking out the clear solution, adding 4 times of anhydrous ethanol, stirring for 24h, precipitating, and vacuum drying at-80 ℃ to obtain Hibiscus tin-orchid Merrill polysaccharide;
(4) and (2) adding 105mg of the double-split sea sedge polysaccharide obtained in the step (2), 105mg of the Ceylophora hybrida polysaccharide obtained in the step (3), 300mg of hyaluronic acid, 4.5g of glycerol and 24.9g of deionized water into 90mg of the Neurospora ferruginea water extraction and alcohol precipitation supernatant part obtained in the step (1) in sequence, stirring, sealing and packaging to obtain the mask containing the Neurospora ferruginea extract.
Example 3
A facial mask containing herba Nepetae Divaricati extract (per 30 g) comprises the following components:
a method for preparing facial mask containing herba Nepetae Divaricati extract comprises the following steps:
(1) preparation of the kohlrabi extract:
① cleaning fresh herba Nerii, draining, and pulverizing into herba Nerii powder;
② mixing pulverized herba Nepetae Divaricati powder with distilled water at a ratio of 1g to 30mL, extracting with water bath at 90 deg.C for 2 hr twice, and filtering to obtain filtrate;
③ concentrating the filtrate under reduced pressure to 43% of the original volume at 65 deg.C and-0.09 MPa, adding anhydrous ethanol to make the volume fraction of anhydrous ethanol in the mixed system 75%, precipitating with ethanol at 4 deg.C for 24h, and centrifuging at 4000r/min for 5min to obtain supernatant and ethanol precipitate;
④ at 65 deg.C and-0.09 MPa, vacuum concentrating the supernatant, and vacuum freeze drying at-80 deg.C to obtain herba Nepetae Divaricati water extract and ethanol precipitation supernatant;
(2) preparing polysaccharide of the double-split sea sedge: soaking the double-split sea sedge powder in 0.15mol/L hydrochloric acid solution, stirring for 72h, centrifuging at the rotating speed of 4500r/min for 10min, taking out the clear solution, adding 2 times of volume of absolute ethyl alcohol, stirring for 24h, precipitating, and vacuum drying at-80 ℃ to obtain double-split sea sedge polysaccharide;
(3) preparing the tin blue sea agaric polysaccharide: soaking the Hibiscus tin-orchid Merrill powder in 0.15mol/L hydrochloric acid solution, stirring for 72h, centrifuging at the rotating speed of 4500r/min for 10min, taking out the clear solution, adding 2 times of anhydrous ethanol, stirring for 24h, precipitating, and vacuum drying at-80 ℃ to obtain Hibiscus tin-orchid Merrill polysaccharide;
(4) and (2) sequentially adding 300mg of the double-split sea sedge polysaccharide obtained in the step (2), 300mg of the Ceylophora hybrida polysaccharide obtained in the step (3), 180mg of hyaluronic acid, 5.52g of glycerol and 23.4g of deionized water into 300mg of the water-extraction alcohol-precipitation supernatant part of the Neurospora hybrida obtained in the step (1), stirring, sealing and packaging to obtain the mask containing the Neurospora hybrida extract.
Second, description of Effect (all take embodiment 1 as an example)
1. Whitening effect of facial mask
Different mass concentrationsThe effect of the water-extraction and alcohol-precipitation supernatant part of the Nectria speciosa on the tyrosinase activity is shown in FIG. 1. As shown in figure 1, the water extraction and alcohol precipitation supernatant of the gynura segetum has stronger inhibition effect on the activity of the tyrosinase, the inhibition rate of the tyrosinase is (24.51 +/-2.04)% -88.37 +/-5.43)%, and the semi-inhibition concentration IC of the gynura segetum on the activity of the tyrosinase is calculated by fitting a nonlinear equation within the mass concentration of 0.25-6.0 mg/mL50It was 1.27 mg/mL.
In example 1, the mass concentration of the water extraction and alcohol precipitation supernatant part of the sedum aizoon is about 0.5mg/mL (higher than 0.25 mg/mL), and it is reasonable to speculate that the water extraction and alcohol precipitation supernatant part of the sedum aizoon contained in the invention has the tyrosinase activity inhibition effect at the lowest concentration within the design of the invention. The key enzyme in the skin melanin synthesis process is tyrosinase, which can catalyze L-tyrosine to generate dopa, dopa is gradually oxidized to generate dopaquinone, and then pigment particles are formed, and the hyperpigmentation of the skin can be caused by the excessive expression of the tyrosinase. Therefore, modern whitening cosmetics and skin drugs are mostly tyrosinase inhibitors. The water extraction and alcohol precipitation supernatant part of the gynura segetum has certain tyrosinase inhibition effect, and the tyrosinase inhibition effect indicates that the gynura segetum has certain effect on whitening.
2. Antioxidant effect of facial mask
(1) The scavenging effect of the supernatant part of the water extraction and alcohol precipitation of the kojic sauerkraut with different mass volume concentrations on the dibenzobitter acyl free radical (DDPH) is shown in figure 2. In the concentration range of 0.4-2.0 mg/mL, the clearance rate of the supernatant part of the water extraction and alcohol precipitation of the gynura segetum to the dibenzo bitter acyl free radicals is about 25-73%, and the half-effect concentration is EC50It was 1.18 mg/mL. In example 1, the mass concentration of the water extraction and alcohol precipitation supernatant part of the gynura segetum is about 0.5mg/mL (higher than 0.4 mg/mL), and it is reasonably speculated that the water extraction and alcohol precipitation supernatant part of the gynura segetum contained in the invention has a function of removing dibenzo bitter acyl radicals at the lowest concentration in the design of the invention, so that the antioxidant effect can be achieved.
(2) Liaw ET al (Liaw ET, Ruan YA, Huang CCJ. Glycemic alignment and characteristics of aqueous extract from Sarcodia cerylanica Procedented materials, 7th Taiwan-Thailand biological conference. Pingtunging, Taiwan: national Pingtunging University of Science and Technology; 2012) study found that an aqueous extract of the double split sea fungus contains polysaccharides (370mg/g) and has an antioxidant effect with a clearance of the dibenzoyl radical of 68.03% at a mass volume concentration of 10mg/mL (corresponding to 3.7mg/mL of polysaccharides). The minimum concentration of the double-split sea ear polysaccharide added in the invention is about 5mg/mL (higher than 3.7mg/mL), so that the reasonable inference shows that the double-split sea ear polysaccharide contained in the invention has the function of removing dibenzo bitter acyl free radicals and has the antioxidant effect under the minimum concentration in the design of the invention.
(3) Luo et al (Luo A, Feng X, Liu Y, Fan Y. polysaccharides from Sarc. cerevisiae and the anti-oxidant properties in vitro and in vivo. Pharmacologia 2014; 5: 235-.
Third, conclusion
The facial mask containing the acanthus extract, which is disclosed by the invention, is added with the acanthus extract, the agaric double-split polysaccharide and the agaric tin orchid polysaccharide, has the effects of whitening and resisting oxidation, and meets the functional requirements of consumers on facial mask products.
The above embodiments and drawings are not intended to limit the form and style of the present invention, and any suitable changes or modifications thereof by those skilled in the art should be considered as not departing from the scope of the present invention.
Claims (6)
1. A facial mask containing a herba Nepetae Divaricati extract is characterized in that: the paint comprises the following components in percentage by mass:
the acanthus ilicifolius extract is an acanthus ilicifolius water extraction and alcohol precipitation supernatant part obtained by extracting acanthus ilicifolius through a water extraction and alcohol precipitation method, the agaricus bisporus polysaccharide is obtained by extracting acanthus bisporus powder, and the agaricus hybridus polysaccharide is obtained by extracting agaricus bisporus powder.
2. The mask containing the extract of kombucha as claimed in claim 1, wherein: the paint comprises the following components in percentage by mass:
3. the method for preparing a mask containing the extract of kombucha as claimed in claim 1, wherein the method comprises the steps of: the method comprises the following steps:
(1) preparation of the kohlrabi extract:
① cleaning fresh herba Nerii, draining, and pulverizing into herba Nerii powder;
② mixing pulverized herba Nepetae Divaricati powder with distilled water at a ratio of 1g to 30mL, extracting with water bath at 90-100 deg.C for 1-2 hr twice, and filtering to obtain filtrate;
③ concentrating the filtrate under reduced pressure to 23-43% of the original volume of the filtrate, adding anhydrous ethanol to make the volume fraction of the anhydrous ethanol in the mixed system be 75%, precipitating with ethanol at 4 deg.C for 24h, centrifuging to obtain supernatant and ethanol precipitate;
④ concentrating the supernatant under reduced pressure, and vacuum freeze drying to obtain herba Nerii water extract and ethanol precipitation supernatant;
(2) preparing polysaccharide of the double-split sea sedge: soaking the double-split sea sedge powder in 0.15mol/L hydrochloric acid solution, stirring, centrifuging, taking out a clear solution, adding 2-4 times of volume of absolute ethyl alcohol, stirring, precipitating, and performing vacuum freeze drying to obtain double-split sea sedge polysaccharide;
(3) preparing the tin blue sea agaric polysaccharide: soaking the tremella mesenterica powder in 0.15mol/L hydrochloric acid solution, stirring, centrifuging, taking out a clear solution, adding 2-4 times of anhydrous ethanol, stirring, precipitating, and performing vacuum freeze drying to obtain the polysaccharide of the tremella mesenterica;
(4) adding the polysaccharide of the agaric bifida obtained in the step (2), the polysaccharide of the agaric glaucopiae obtained in the step (3), hyaluronic acid, glycerol and deionized water into the supernatant part of the water extraction and alcohol precipitation of the acanthopanax japonicus obtained in the step (1) in sequence, wherein the mass ratio of the polysaccharide to the hyaluronic acid to the glycerol is that the supernatant part of the water extraction and alcohol precipitation of the acanthopanax japonicus: double split sea ear polysaccharide: polysaccharide of the fungus Stylosanthes guianensis: hyaluronic acid: glycerol: 0.05-1% of deionized water: 0.5-1: 0.5-1: 0.2-1: 10-20: 78-88, stirring, sealing and packaging to obtain the mask.
4. The method for preparing a mask containing a kombucha extract as claimed in claim 3, wherein the method comprises the steps of: in the step (1), the reduced pressure concentration is carried out at 65 ℃ and-0.09 MPa, the rotation speed of the centrifugation is 4000r/min, the time of the centrifugation is 5min, and the temperature of the vacuum freeze drying is-80 ℃.
5. The method for preparing a mask containing a kombucha extract as claimed in claim 3, wherein the method comprises the steps of: in the step (2), the stirring time for the first time is 72 hours, the stirring time for the second time is 24 hours, the rotation speed of the centrifugation is 4500r/min, the time of the centrifugation is 10 minutes, and the temperature of the vacuum freeze drying is-80 ℃.
6. The method for preparing a mask containing a kombucha extract as claimed in claim 3, wherein the method comprises the steps of: in the step (3), the stirring time for the first time is 72 hours, the stirring time for the second time is 24 hours, the rotation speed of the centrifugation is 4500r/min, the time of the centrifugation is 10 minutes, and the temperature of the vacuum freeze drying is-80 ℃.
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| WO2010041832A3 (en) * | 2008-10-07 | 2010-07-29 | 부경대학교 산학협력단 | Antioxidant active composition containing an ishige okamurae-derived compound |
| KR20100111066A (en) * | 2009-04-06 | 2010-10-14 | 재단법인 제주테크노파크 | Skin whitening composition |
| JP6242575B2 (en) * | 2013-01-09 | 2017-12-06 | 御木本製薬株式会社 | Dendritic elongation inhibitor |
| KR20140147280A (en) * | 2013-06-19 | 2014-12-30 | 한밭대학교 산학협력단 | Cosmetic composition for improving skin aging and wrinkle comprising extract of Ishige okamurae by n-butanol |
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