KR20100062291A - 크리포넥트리아 파라시티카 유래의 신규한 프로모터 및 이의 용도 - Google Patents
크리포넥트리아 파라시티카 유래의 신규한 프로모터 및 이의 용도 Download PDFInfo
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- KR20100062291A KR20100062291A KR1020080120857A KR20080120857A KR20100062291A KR 20100062291 A KR20100062291 A KR 20100062291A KR 1020080120857 A KR1020080120857 A KR 1020080120857A KR 20080120857 A KR20080120857 A KR 20080120857A KR 20100062291 A KR20100062291 A KR 20100062291A
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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Abstract
Description
Claims (10)
- 크리포넥트리아 파라시티카 (Cryphonectria parasitica) 유래의 크리파린(cryparin) 유전자의 전사개시점으로부터 83bp 단편과 전사체의 5'-비해독 영역 105bp 단편으로 이루어진 프로모터.
- 제1항에 있어서, 상기 프로모터는 서열번호 1로 표시되는 염기서열을 갖는 것을 특징으로 하는 프로모터.
- 제1항 또는 제2항의 프로모터와 목적단백질을 코딩하는 유전자를 포함하는 재조합 벡터.
- 제3항에 있어서, 진균용인 것을 특징으로 하는 재조합 벡터.
- 제4항에 있어서, 상기 벡터는 도 1의 pCHPH1, pCLAC3, pCGO 또는 pCARGB1인 것을 특징으로 하는 벡터.
- 제3항의 재조합 벡터로 형질전환된 미생물.
- 제6항에 있어서, 상기 미생물은 진균인 것을 특징으로 하는 미생물.
- 제7항에 있어서, 상기 진균은 크리포넥트리아 파라시티카 (Cryphonectria parasitica), 아스퍼질러스 나이거 (Aspergillus niger), 지베렐라 제아에 (Gibberella zeae) 또는 아스퍼질러스 니둘란스 (Aspergillus nidulans)인 것을 특징으로 하는 미생물.
- 제6항의 미생물을 배양하는 것을 특징으로 하는 목적단백질의 제조방법.
- 제9항에 있어서, 상기 미생물은 진균인 것을 특징으로 하는 목적단백질의 제조방법.
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KR1020080120857A KR101038900B1 (ko) | 2008-12-02 | 2008-12-02 | 크리포넥트리아 파라시티카 유래의 신규한 프로모터 및 이의 용도 |
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KR1020080120857A KR101038900B1 (ko) | 2008-12-02 | 2008-12-02 | 크리포넥트리아 파라시티카 유래의 신규한 프로모터 및 이의 용도 |
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KR20100062291A true KR20100062291A (ko) | 2010-06-10 |
KR101038900B1 KR101038900B1 (ko) | 2011-06-03 |
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