KR20100000380A - Mixed medicinal herb extracts having anti-inflammatory activity and cosmetic composition containing the same - Google Patents

Abstract

PURPOSE: A cosmetic composition containing medicinal herb mixture is provided to ensure anti-inflammation and anti-oxidation. CONSTITUTION: A medicinal herb mixture contains 20-25 weight% of Forsythia Fruit, 15-18 weight% of Cimicifugae Rhizoma, 15-18 weight% of Puerariae Radix, 15-18 weight% of Platycodi Radix, 15-18 weight% of Glycyrrhizae Radix, and 10-13 weight% of Paeonia Radix. The medicinal herb extract contain actin, arctigenin and matairesinol as an active ingredient for anti-inflammation. The active ingredient contains 2.5-5.4 weight% of actin, 4-5 weight% of arctigenin, and 4.8-7 weight% of matairesinol. The cosmetic composition is used in the form of essence, oil, cream, powder, pack, foundation, makeup base, or stick.

Description

Mixed medicinal herb extracts having anti-inflammatory activity and cosmetic composition containing the same}

The present invention relates to a herbal medicine extract having an anti-inflammatory activity and a cosmetic composition containing the same, more specifically, antioxidant activity, anti-inflammatory activity and skin moisturizing and maintenance activity obtained by extracting from medicinal herb, horse riding, horse root, gilyeong, licorice and medicinal herb It relates to a mixed herbal medicine extract having a and a cosmetic composition for improving skin troubles and atopic skin containing the same as an active ingredient.

Inflammation is a series of defenses aimed at minimizing the response and restoring the damaged area when a cell or tissue is damaged by any cause.It causes nerves and blood vessels, lymphatic vessels, humoral responses, and cellular reactions, resulting in pain, It causes swelling, redness, and fever, causing dysfunction. Inflammation may be caused by physical factors such as trauma, injury, frostbite, chemical factors by chemicals, immunological factors by antibody reactions, and also by blood vessel or hormone imbalance. Vasodilation is caused by various chemical mediators released by cells damaged by external stimuli, and as permeability increases, antibodies, complement, plasma, and phagocytic cells flock to the inflammatory site. This phenomenon causes erythema.

The production of mediators that promote the migration of leukocytes along with inflammation is also accomplished by the process through arachidonic acid. This pathway consists of cyclooxygenase and lipoxygenase, and leukotriene and prostaglandin produced by these enzymes act as inflammation-inducing factors. Therefore, inhibitors of lipoxygenase and cyclooxygenase may exhibit anti-inflammatory activity as a substance that inhibits cell membrane destruction and lipid peroxidation reaction caused by free radicals generated during inflammation as well as inhibiting the production of inflammatory substances. In addition, by inhibiting proteins and lipolytic enzymes produced by excessive inflammation, it is possible to prevent skin aging by protecting the damaged cells thereby.

When dry skin is formed, the barrier function of the skin surface is lost and the skin's invasion of irritants or antigens from the outside is facilitated, and the skin reacts to these invaders, and the skin is rejected. The reaction occurs when keratinocytes, which make up the majority of the stratum corneum, activate keratinocytes, such as Langerhans and melanocytes, which produce cytokines. It is also known to appear.

Anti-inflammatory agents are called anti-inflammatory agents that act to eliminate inflammation, reduce biological response and symptoms to eliminate inflammation. Although allatoin, azene, hydrocortisone, licorice acid and its derivatives (β-glycileic acid, glycylinic acid derivatives) are known to be effective in anti-inflammatory activity (Neo Cosmetics, Namsan Sugar, p162, 1993), The amount of use thereof is limited, or the cosmetic formulation has most problems in terms of stability, so its use is limited.

Conventionally, attempts have been made to use herbal medicines with less side effects on the human body for the treatment of inflammation. Korean Patent Application No. 10-2000-0044592 discloses a herbal composition having an anti-inflammatory effect obtained from three hundred seconds and fishery herb in the "anti-inflammatory composition of the anti-inflammatory effect", the Korean Patent Application No. 10-1998-0027093 It has been shown to have an anti-inflammatory effect. In addition, Korea Patent Application No. 10-2003-0050468 "Cosmetic formulations (licorice, sugar, green tea, Cheongung, ginseng, aloe) and amino acid polymer (polyglutamic acid) cosmetic composition using" cetanol, surfactants, liquid paraffin, A composition having a moisturizing effect, an antibacterial and anti-inflammatory effect on a skin prepared by containing a herbal medicine extract such as licorice, sugar, green tea, cheon gung, ginseng, and aloe and an amino acid polymer is disclosed. 10-2003-0028350 discloses a cosmetic composition comprising vitamin C and one or more herbal extracts selected from the group consisting of green tea, phosphorus mugwort and pine needles in an “cosmetic composition having an antioxidant and anti-inflammatory effect”. In addition, attempts have been made to use various herbal medicines known to have antioxidant and anti-inflammatory effects.

Yeongyo (連翹, Forsythia Fruit) is Forsythia belonging to the Oleaceae (Oleaceae) (Forsythia viridissima L.). Forsythia is deciduous shrub, 2 ~ 3m high, thin and long branch, spread or draped down, hollow. Flowers bloom before leaves, but 1 to 4 flowers grow on leaves. Fruits are narrow egg-shaped, with short beak at the end, and split into 2 pieces when fully cooked. Grows in wild wilderness, and is harvested when the fruit is ripe or when it is fully ripe and dried in the sun. The constituents of ducts are known as actinigen as lignan, actin as lignan glycoside, and caffeic acid glycoside. It is used as an anti-inflammatory and drainage skin disease in oriental medicine, and grows in Korea and China.

Riding (升麻, Cimicifugae Rhizoma) Why is a dongsok plants of Ranunculaceae perennial that grows in the forest and horseback riding (Cimicifuga japonica S., horseback riding ( Cimicifugae) dahurica M.), horseback riding ( Cimicifuga simplex T.), stork riding ( Cimicifugae foetida L.). From ancient times it has been used for medicinal purposes, causing fever, edema and hives. In July-August, the small white flowers of the stem are layered and stacked.The roots are visamminol, visnagin, cimifugin, cycloartene, and triter. Triterpenoids such as cimigenol, cimigenol-3-xyloside, cihugenol, dahurinol, cimicifugenol, acetylsegmanol xyloxide ( There are acetylshegmanolxyloside, isoferulic acid, and ferulic acid.

Puerariae Radix is the root of the perennial herb, Pueraria lobata O., which grows in the mountains of Korea, China, and Japan. In Chinese medicine, the various parts of the 칡 are classified, and the dried root of 칡 is called root, the flower is brown, the fruit is brown or brown, the leaves are brown, and the vine is brown, and it has been used differently depending on the symptoms and treatment of the disease. . In the oriental medicine, the roots are used to relieve the muscles, so it is used for the purpose of releasing cold body swelling, rash, anti-alcoholic pain, etc. Governor acts. Its main ingredient contains a large amount of isoflavonoide deoxymiroestrol and puerarin. The pharmacological action associated with cartilage is known as anti alcoholic (antidipsotropic) action, hepatoprotective action, hangover inhibitory action, antioxidant activity.

Platycodi Radix is a perennial herbaceous Platycodon belonging to the Campanulaceae. The roots of grandiflorum A. DC.) are dried. A white bellflower that grows or grows in Korea. Platycodon grandiflorum for . albiflorum H.), the layers of the Terry bellflower (Platycodon grandiflorum for. duplex M.) , of which white flowers, huingyeop bellflower (Platycodon grandiflorum for . leucanthum H.). The components include triterpenoid saponins and sterols, and other sugars include inulin and platinum codinin. The main effect of bellflower in herbal medicine is to cough, sputum, etc. It is also good for symptoms such as sore throat, sound loss, difficulty urination, diarrhea, and post-heavy weight.

Licorice (Glycyrrhizae Radix) is a perennial herb belonging to the legume (Leguminosae), and European licorice ( Glycyrrhiza) glabra L.), Glycyrrhiza uralensis F.) or other similar plant roots and outgrowths (또는 出 莖) intact or removed. Pharmacological action of licorice with adrenal cortex hormone action, anti-inflammatory activity and anti-allergic action, action on digestive system, detoxification action, lipid metabolism, effect on experimental jaundice, antitussive action, analgesic and anticonvulsive action, urinary tract And the effects on the reproductive system, anti-tumor action, other weight gain action, muscle strength enhancer action, blood pressure increase action, hemolysis action, fungal action, antibacterial action, antipyretic action, insect repellent action was found. It is mainly used for medicinal roots and creep, and the main medicinal ingredients contain 3.63 ~ 13.06% of glycyrrhizic acid, triterpenoid and saponin, and other kinds of flavone compounds There is this.

Paeonia japonica (白芍藥, Radix Paeonia) is a perennial seconds of peony and peony (Paeoniaceae) (Paeonia albiflora P. and Paeonia lactiflora P.) and Paeonia obovata M.). Count is a white, blood, sedative, gynecological and external medicine. It contains 35 genera and 1,500 species, which are distributed throughout the world, but mainly in the temperate and boreal regions of the Northern Hemisphere, Korea, Japan, China, and Sakhalin Island. There are 106 genera of 21 genera. Ingredients include paeoniflorin, paeonol, paeonine, tannin, benzoic acid, benzoic acid, essential oils, resins, sugars, and the like.

The blended herbal medicine consisting of Yeongyo, Horse Riding, Brown Root, Gilgyeong, Licorice, and Earl's Medicine is a prescription group recorded in the traditional Chinese medicine book. It is an anti-histamine effect that is originally used when small beads occur in the early stages of smallpox. In the case of atopic patients, the lesion site is eczema, and the local secondary infection is found. It is known to use when seen.

However, until now, no attempt has been made to determine the antioxidant and anti-inflammatory effects of extracts by producing extracts by mixing a certain amount of ducts, horseback riding, rooting, gilyeong, licorice, and ear cures.

The inventors have identified the advantages and disadvantages of attempts to obtain anti-inflammatory activity using various herbal medicines in the prior art to solve the conventional problems and maintain the antioxidant activity, anti-inflammatory activity, and skin moisturizing to improve skin troubles and atopic skin. As a result of thorough research to search for the substances present, 11 kinds of herbal medicines were searched in the literature and selected mixed medicinal herbs that were most likely to be related to atopic symptoms were investigated. Inhibition of lipoxygenase activity, inhibition of nitric oxide (NO) production, and inhibition of prostaglandin synthesis resulted in an excellent anti-inflammatory effect and led to the present invention.

Therefore, an object of the present invention is an excellent herbal medicine extract with excellent antioxidant activity, anti-inflammatory activity and skin moisturizing effect extracted from medicinal herb of Yeongyo, Horse Riding, Root, Gilt, Licorice and Earl, and a cosmetic composition comprising the same as an active ingredient and the same It is to provide a used cosmetics.

An object of the present invention as described above is selected, quantitatively mixed with Yeongyo, horse riding, rooting, Gilyeong, licorice, and Earl, after preparing a herbal medicine mixed extract therefrom, the antioxidant activity effect and inflammation-inducing substance of the extract Phosphorus NO, PGE ₂ , was achieved by confirming the anti-inflammatory activity and skin moisturizing maintenance effect through inhibition of TNF-α production can be used as a cosmetic for improving skin troubles and atopic skin.

The present invention provides a mixed herbal extract of ducts, horseback riding, rooting, gilpyeong, licorice, and earl of the currant having antioxidant activity, anti-inflammatory activity and skin moisturizing maintenance activity.

The mixed herbal medicine extract according to the present invention is 20 to 25% by weight, 15 to 18% by weight, 15 to 18% by weight, 15 to 18% by weight, 15 to 18% by weight, licorice 15 to 18% by weight and 10 to 13 weight In percent.

The mixed herbal medicine extract according to the present invention preferably comprises 23.3% by weight of Yeongyo, 16.3% by weight of horse riding, 16.3% by weight, length of 16.3% by weight, licorice 16.3% by weight and 11.5% by weight of larch.

The mixed herbal extract according to the present invention contains actin, actinigen and matyrethinol as anti-inflammatory active ingredients.

The mixed herbal extract according to the present invention contains 2.5 to 5.4% by weight of actin, 4 to 5% by weight of actinigen and 4.8 to 7% by weight of matyrethinol as anti-inflammatory active ingredients.

The present invention provides a method of producing a mixed herbal medicine extract of duct bridge, horse riding, rooting, gilyeong, licorice, and earl of the anti-inflammatory and anti-inflammatory activity.

Method for producing a mixed herbal medicine extract according to the present invention comprises the steps of extracting the selected herbal medicines of a selected ratio of Yeongyo, horse riding, rooting, Gilyeong, licorice, and Earl of a certain number of extracts; Filtering the obtained extract filtrate and concentrating under reduced pressure to obtain a primary extract; Dissolving the primary extract, and then fractionating it to support it; And partially concentrating the obtained lower layer under reduced pressure, and then extracting fractions by adding an extraction solvent to obtain a final extract.

At this time, the content of each of the duct bridge, horseback riding, rooting, gilyeong, licorice, and earl medicinal herbs contained in the mixed herbal medicine, 20 to 25% by weight, 15 to 18% by weight of horse riding, 15 to 18% by weight relative to the total weight of the herbal medicine, Preference is given to 15-18% by weight, 15-18% by weight licorice and 10-13% by weight of earluff.

In addition, the extraction solvent used to obtain the primary extract is from a solvent group including purified water, anhydrous or hydrous methanol having 1 to 4 carbon atoms, ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol and butylene glycol One pure solvent selected or a mixed solvent combining two or more may be used, but is not limited thereto. The amount of the extraction solvent added is preferably 1 to 20 times the total volume of the mixed herbal medicines. This extraction step may be performed by applying a conventional extraction method of the herbal medicine, which will be apparent to those skilled in the art having ordinary knowledge in the art.

The present invention provides a cosmetic composition for improving skin troubles and atopic dermatitis, comprising a mixed herbal extract of ducts, horse riding, rooting, gilyeong, licorice, and earl pear as an active ingredient.

The cosmetic composition according to the present invention preferably comprises 0.001 to 30% by weight of the mixed herbal extracts relative to the total weight.

If the content of the extract is less than 0.001% by weight relative to the total weight of the cosmetic composition, it is not preferable to achieve a sufficient skin trouble and atopic skin improvement effect originally, it is not preferable, if the content exceeds 30% by weight of cosmetic stability It may cause a problem, and this is not preferable because of the drawback of the cosmetic appearance or the use of a sufficient cosmetic effect when using.

In another aspect, the present invention provides a cosmetic for improving skin troubles and atopic skin prepared with the cosmetic composition.

Cosmetics according to the present invention, manufactured by using the cosmetic composition, such as cosmetics, makeup products, cosmetics, essences, oils, creams, powders, packs, foundations, makeup bases, sticks, etc. It can be prepared and used as a dosage form, and can also be applied in various forms such as liquid, cream, paste, and solid, and can be prepared by a conventional cosmetic preparation method, but is not limited thereto. Those skilled in the art will be able to make various applications using various methods.

As described above, the herbal extracts obtained from the mixed herbal medicines of Yeongyo, Horse Riding, Root, Gilt, Licorice and Earl of the present invention have antioxidant activity and anti-inflammatory activity through inhibition of NO, PGE ₂ and TNF-α production as inflammatory substances. It has an effect, and also has an excellent skin moisturizing effect, and can be effective as a cosmetic composition for improving skin troubles and atopic skin and is a very useful invention in the cosmetic industry.

Hereinafter, preferred embodiments of the present invention will be described in more detail with reference to Examples. However, the scope of the present invention is not limited to these examples.

Example of the present invention The used bridge, horseback riding, rooting, gilyeong, licorice and earl medicament were purchased from agricultural and forestry medicine, each reagent used a first-class product.

<Example 1>

50 g of selected bridges were placed in an extractor, and 1 L of a 75% ethanol aqueous solution was added as an extraction solvent, followed by reflux cooling extraction twice for 4 hours. The obtained filtrate was filtered through Whatman No. 2 filter paper and concentrated under reduced pressure with a rotary evaporator to obtain 75% ethanol extract.

Distilled water and 10% aqueous ethanol solution were added to the ethanol extract, and then dissolved in fractions with normal-hexane. The lower layer obtained at this time was concentrated under reduced pressure, ethyl acetate was added thereto, and the residue was concentrated to obtain an ethyl acetate extract.

<Examples 2 to 6>

Ethyl acetate extracts were obtained using the same method as the above except that the raw materials were horseback riding, brown root, gilyeong, licorice, and white peach.

<Example 7>

Ethyl acetate was prepared in the same manner as in Example 1, except that 50 g of the mixed raw liquid material obtained by mixing 11.64 g of raw material, 8.14 g of horse riding, 8.14 g of root, 8.14 g of liquorice, 8.14 g of licorice, and 5.80 g of Baekja was used as a raw material of herbal medicine. An extract was obtained.

<Examples 8 to 13>

Except for using dichloromethane as the extraction solvent, using the same method as in Example 1 to obtain the respective dichloromethane extract from the duct bridge, horseback riding, brown root, gilyeong, licorice, white currant as raw materials for herbal medicine.

<Example 14>

Except for using dichloromethane as the extraction solvent, using the same method as in Example 1, 11.64 g, horse riding 8.14 g, brown root 8.14 g, 8.14 g long licorice 8.1. Dichloromethane extract was obtained from 50 g of the obtained mixed liquid material.

The yields of the herbal extracts according to Examples 1 to 14 are shown in Table 1 below.

Experimental Example 1

In order to examine the antioxidant activity effect of each herbal extract prepared in Examples 1 to 14, a free radical scavenging activity test was performed. The free radical scavenging activity test is a modification of the method of Kim et al . ( Kor. J. Pharmacogn. , 24 (4), 299-303, 1993), which is a stable free radical DPPH (1,1-diphenyl-2-picryl hydrazyl, Sigma) reagent was used.

First, each sample obtained in Examples 1 to 14 in 1 mL of 0.2 mM DPPH solution was diluted in methanol at an appropriate concentration and mixed in 2 mL, and left for 10 minutes at room temperature, and then absorbance was measured at 517 nm. On the other hand, the control in the free radical scavenging test was measured by the same method by adding methanol instead of the sample solution, it was set to obtain a color correction value for each of the examples by putting ©© ethanol instead of DPPH solution.

The free radical scavenging ratio was calculated numerically using Equation 1 shown in Table 2 below. In Table 2 below, SC ₅₀ is a concentration of the sample required to achieve 50% free radical scavenging ratio, which is a numerical value used in comparison with other component substances, indicating that the smaller the value, the higher the scavenging ratio. Experiments were performed three times each and expressed as mean ± standard deviation.

The active oxygen scavenging test is a modification of the method of Noro et al . ( Chem . Pharm . Bull ., 31, 3984 ~ 3987, 1983), and the evaluation of the superoxide anion scavenging activity was performed by xanthine / xanthine oxidase. A change in absorbance due to oxidation of nitroblue tetrazolium (NBT) due to active oxygen was measured using an active oxygen generation system by enzyme reaction.

2.4 mL of Na ₂ CO ₃ , 0.1 mL of xanthine, 0.1 mL of EDTA, 0.1 mL of BSA, 0.1 mL of NBT, and 0.1 mL of sample were added well and left to stand at 25 ° C. for 10 minutes. After adding 0.1 mL of Xanthine oxidase and reacting at 25 ° C. for 20 minutes, 6 mM CuCl ₂ was added to stop the reaction, and the absorbance was measured at an optical wavelength of 560 nm. On the other hand, the control group in the active oxygen scavenging activity test was measured by the same method to put the purified water instead of the sample solution, it was set to obtain the color correction value for each of the examples by adding purified water instead of xanthine oxidase solution.

Using the following equation (2) to calculate the active oxygen scavenging rate as a numerical value is shown in Table 2 and the experiment was performed three times each to represent the average value ± standard deviation. In Table 2 below, IC ₅₀ is a concentration of a sample required to achieve 50% of the active oxygen scavenging rate, which is a value used in comparison with other component substances.

<Equation 1>

<Equation 2>

TABLE 2

As shown in Table 2 above, in the results of the free radical scavenging activity test, Examples 1 to 7 showed somewhat better scavenging activity than those of Examples 8 to 14. In the individual medicinal herb extracts, Examples 6 and 13 of Baekjak showed excellent free radical scavenging activity, respectively, and showed the best free radical scavenging activity in Examples 7 and 14, which are mixed herbal extracts, as compared to the individual herbal extracts.

On the other hand, in the result of active oxygen scavenging activity test, the mixed herbal medicine extracts were slightly better than the individual herbal extracts in Examples 7 and 14, but all of the examples showed similar levels of active oxygen scavenging activity.

Experimental Example 2

Inhibition of activity against lipoxygenase was measured to confirm the anti-inflammatory activity of each herbal extract prepared in Examples 1 to 14. 5-lipoxygenase adds molecular oxygen to unsaturated fatty acids having 1,4-cis, cis-pentadiene (1,4-pentadiene) structures in the molecule, such as linoleic acid, linolenic acid, and arachidonic acid. It is an oxidase that produces cis, trans-diene 5-hydroperoxide and is involved in the first reaction of leucotriene biosynthesis from arachidonic acid to various inflammatory and allergic diseases. Therefore, the anti-inflammatory effect can be evaluated by measuring the inhibitory activity of lipoxygenase using soyben lipoxygenase, which is structurally and mechanism similar to lipoxygenase.

In 1 mL of 1 mM linoleic acid, 0.9 mL of sample (10 mg / mL) and 0.9 mL of lipoxygenase dissolved in 0.1 M sodium phosphate buffer (pH 7.0) were added, followed by reaction for 10 minutes in a 25 ° C water bath. 0.5 mL of 20% (w / v) trichloroacetic acid was added to terminate the reaction. Then, 1 mL of 0.6% (w / v) TBA was added and developed in boiling water for 10 minutes. Thereafter, the mixture was cooled in ice water for 2 minutes, 2 mL of n-butanol was added thereto, and the mixture was shaken and then centrifuged at 3,500 rpm for 5 minutes. The anti-inflammatory activity effect (%) was evaluated by taking the upper layer (n-butanol layer) and measuring the absorbance at 535 nm.

The control group for the anti-inflammatory activity test was measured in the same manner by adding a buffer solution instead of the sample solution, and was set to the case where each color correction value for the sample and the control group was added to the buffer solution instead of lipoxygenase. The anti-inflammatory activity was calculated numerically by using Equation 3 below, and the results are shown in Table 3 below. The experiments were performed three times, respectively, and are expressed as mean ± standard deviation. The numerical values shown in Table 3 below are inhibition rates for concentrations of 10 mg / mL in Examples 1-14.

<Equation 3>

TABLE 3

As shown in Table 3, the concentration (IC ₅₀ ) value that can inhibit 50% of the lipoxygenase activity of each of the embodiments was not available, but overall Examples 8 to 14 Examples 1 to 7 It showed a relatively good inhibitory effect. In the individual medicinal extracts, Examples 5 and 12 of licorice showed excellent inhibitory effect, respectively, and Examples 7 and 14, which were mixed herbal extracts, showed the best inhibitory effect.

In the results of Experimental Examples 1 and 2, the antioxidant and anti-inflammatory activity assays showed superior antioxidant and anti-inflammatory activity in the mixed herbal extracts than the individual herbal extracts.

Experimental Example 3

An indicator component (active ingredient) showing the physiological activity of the mixed herbal medicine extracts obtained in Examples 7 and 14 was analyzed. After primarily investigating the main components of the herbal medicines, as shown in Table 4, compounds known to have anti-inflammatory activity were analyzed by high performance liquid chromatography (HPLC), and the analysis conditions are as described below.

Chromatography: Dionex Summit HPLC

Column: Luna C ₁₈ (250 mm × 4.6 mm × 0.5 μm)

Detector: UV absorbance photometer (wavelength 329 nm)

Flow rate: 1.0 mL / min

Mobile phase: gradient elution of 0.1% acetic acid solution dissolved in acetonitrile and 0.1% acetic acid solution dissolved in water

TABLE 4

As a result of analyzing the examples under the above conditions, actin, actigenin, and matairesinol were analyzed as shown in Table 5 below. The analysis was performed three times each and expressed as the mean value ± standard deviation.

TABLE 5

The results shown in Table 5 were analyzed for the bioactive components contained in Examples 7 and 14. The content of the active ingredients contained in Example 14 was about 1.3 times higher than that of Example 7, and it was shown to be consistent with the antioxidant and anti-inflammatory activity of Experimental Examples 1-2.

Experimental Example 4

In order to confirm the anti-inflammatory activity of the mixed herbal extracts obtained in Examples 7 and 14, the effects on NO (nitric oxide) production known to play an important role in inducing inflammation were examined. Normal NO formation plays an important role in killing bacteria or removing tumors. However, NO produced by iNOS in inflammatory conditions not only promotes inflammatory reactions such as vascular permeability and edema, but also promotes biosynthesis of inflammatory mediators. It is known to deepen.

RAW264.7 cells were adjusted to 5 × 10 ⁵ cells / well using DMEM medium supplemented with 10% FBS, inoculated into 24 well plates, treated with samples, and cultured for 30 minutes, followed by LPS (lipopolysaccharide, 1 μg / mL). ) Was incubated for 24 hours. The amount of generated NO was calculated by using the NO detection kit (iNtRON) and calculated in Equation 4 below, and the results are shown in Table 6. The experiments were performed three times, respectively, and the average value ± standard error. The control group for the anti-inflammatory activity test was the experimental group treated with LPS alone.

<Equation 4>

TABLE 6

As shown in Table 6, Example 14 showed the inhibitory effect of about 42% and 59% NO production at concentrations of 5 and 10 μg / mL, respectively, and about 1.5 to 2 times more NO production than Example 7. Inhibitory effect was shown.

Experimental Example 5

In order to confirm the anti-inflammatory activity of the mixed herbal extracts obtained in Examples 7 and 14, the effects on the production of prostaglandin E ₂ (prostaglandin E _2, PGE ₂ ) _, which are known to play an important role in inducing inflammation _, were examined. Cyclooxygenase (COX) is an enzyme that converts arachidonic acid to prostaglandins and is classified as COX-1 and COX-2. COX-1 acts on normal biological functions such as platelet formation, gastric wall protection, and renal function maintenance. COX-2 forms inflammatory mediator PGE ₂ . PGE ₂ is known to be deeply involved in cancer, including promoting inflammatory, immune and angiogenesis.

RAW264.7 cells were adjusted to 5 × 10 ⁵ cells / well using DMEM medium added with 10% FBS, inoculated in a 24 well plate, the samples were treated, and after 30 minutes incubation, 1 μg / mL of LPS was added. Incubated for 24 hours. The amount of PGE ₂ produced was calculated by using the PGE ₂ ELISA kit (R & D systems, Inc, USA), and is shown in Table 7 by the following equation. Indicated. The control group for the anti-inflammatory activity test was the experimental group treated with LPS alone.

<Equation 5>

TABLE 7

As shown in Table 7, Example 14 showed an excellent inhibitory effect of PGE ₂ production of about 75% and 90% at concentrations of 5 and 10 μg / mL, respectively, and about 1.4-fold inhibition compared to Example 7. It showed an effect.

Experimental Example 6

In order to confirm the anti-inflammatory activity of the mixed herbal extracts obtained in Examples 7 and 14, the effect on TNF-α production, which is one of the initial inflammatory molecules, was examined. Formation of pro-inflammatory cytokine such as TNF-α leads to the conversion of arachidonic acid to prostaglandin and NO formation due to the activity of phospholipase A2. .

RAW264.7 cells were adjusted to 5 × 10 ⁵ cells / well using DMEM medium supplemented with 10% FBS, inoculated into 24 well plates, the samples were treated, and after 30 minutes of incubation, 1 μg / mL of LPS was added. Incubated for 24 hours. The amount of the generated TNF-α is shown in Table 8 by calculating the value measured using the TNF-α ELISA kit (eBioscience Inc, USA) by the following equation 6, the experiment was performed three times each, the average value ± standard error Represented by. The control group for the anti-inflammatory activity test was the experimental group treated with LPS alone.

<Equation 6>

TABLE 8

As shown in Table 10, Example 14 had a TNF-α production inhibitory effect of about 23% at a concentration of 10 μg / mL and showed a production inhibition effect of about twice that of Example 7.

<Formulation Example 1>

A component composition of the atopy lotion containing the active ingredient material of the cosmetic composition according to Example 14 was prepared as shown in Table 9 below. At this time, the unit of component content is weight%. On the other hand, among the components identified by each component number in Table 9 below, components 2 and 3 were first dispersed and dispersed in component 1 (purified water), and then components 4 to 6 were added, and components 8 to 10 were dissolved in component 7. And component 11 were added in order to prepare a mixture. As a comparative formulation example, the rest of the component constitution except for component 11 and the preparation method thereof were performed in the same manner.

TABLE 9

<Formulation Example 2>

Component composition of the atopic lotion containing the active ingredient material of the cosmetic according to Example 14 was prepared by configuring as shown in Table 10 below. At this time, the unit of component content is weight%. On the other hand, among the components identified by the component numbers in Table 10 below, components 1 to 7 were first dissolved by heating at a temperature of 70 ° C., and then components 8 to 11 were dissolved and dispersed in component 12, and emulsified in those heated to 70 ° C. . Thereafter, the emulsified product was neutralized with component 13, cooled to a temperature of 56 ° C, and then component 14 was added thereto, stirred, and cooled to room temperature.

TABLE 10

<Formulation Example 3>

Component composition of the atopy cream containing the active ingredient material of the cosmetic according to Example 14 was prepared by configuring as shown in Table 11. At this time, the unit of component content is weight%. On the other hand, among the components identified by each component number in Table 11 below, components 1 to 7 were first dissolved by heating at a temperature of 70 ° C., and then components 9 to 12 were dissolved and dispersed in component 13, and emulsified to those heated to 70 ° C. . Thereafter, the emulsified product was cooled to a temperature of 56 ° C., and then component 14 was added thereto, stirred, and cooled to room temperature.

TABLE 11

Experimental Example 7

As a clinical trial for skin moisturization of the cosmetic containing an active ingredient substance according to the present invention, skin moisturizing power was evaluated by measuring skin conductivity using a skin moisture meter (Corneometer, Courage + Khazaka, GmbH, Germany).

Moisturizing power measurement by skin moisture meter is a device that measures the moisture content of skin surface (e.g. stratum corneum) by capacitance measurement method, and the skin surface moisture content measurement is not affected by the sample applied locally. This has the advantage of maintaining a constant measuring level of 30 to 40 μm below the probe sensor contacts.

Skin moisture measurement at room temperature 20 ~ 25 ℃, relative humidity of 40 to 55% having constant temperature and humidity conditions five examinee area of ² × 2 cm 2 to the inside of the arm to the target body part with respect to using the skin moisture measurement in keeping with 7.5 μL of Formulation Example 3 was applied and measured over time.

Using the following equation (7) to calculate the skin conductivity increase rate (%) as a moisturizing power is shown in Table 14 below, one measurement site was measured 5 times each time and expressed as the average value ± standard deviation. In addition, the skin conductivity was measured and defaulted before application of the sample, and the control group was not applied to both the formulation example and the comparative formulation example.

<Equation 7>

TABLE 12

As shown in Table 12, according to the application of Formulation Example 3 containing Example 14, the skin moisturizing power was increased by about 45% compared to the control group did not apply both Formulation Example 3 and Comparative Formulation Example 3. Compared with Comparative Formulation Example 3, skin moisture was increased by about 12%.

Experimental Example 8

In order to determine the occurrence of irritation or allergic reaction through the skin safety, that is, the skin reaction of the mixed herbal medicine extract according to the extraction example 14, a human patch test was performed according to the Korean Food and Drug Administration standard skin safety test method. .

Thirty healthy adult women aged 20 to 50 years were selected as subjects. The patch test was applied to the inner side of the subject's upper arm and the patch was removed after 48 and 72 hours. Each test site was observed after 30 minutes and 24 hours. Criteria are as shown in Table 15 below.

TABLE 13

The skin readiness of the sample was calculated by using Equation 8 below for 30 minutes and 24 hours after patch application.

<Equation 8>

The skin irritation degree of the sample showing the positive response calculated based on the test result of the patch test is shown in Table 14 below.

TABLE 14

As shown in Table 14, Examples 7, 14, according to the skin reactivity determination criteria according to Table 15 in the human patch test showed a good result for use on sensitive skin without irritation. Therefore, Examples 7 and 14 were found to be safe components without skin side effects.

TABLE 15

Claims (7)

Mixed medicinal herb extracts with antioxidant, anti-inflammatory and skin moisturizing activity extracted from mixed medicinal herbs, including ducts, horse riding, rooting, gilgon, licorice and earl The method of claim 1, wherein the mixed herbal medicine extract is 20 to 25% by weight, 15 to 18% by weight, 15 to 18% by weight, 15 to 18% by weight, 15 to 18% by weight, licorice 15 to 18% by weight, and 10 to 13 Count Mixed herbal medicine extract, characterized in that consisting of. The mixed herbal medicine extract according to claim 1, wherein the mixed herbal medicine extract contains actin, actinigen and matyrethinol as anti-inflammatory active ingredients. 4. The mixed herbal extract according to claim 3, wherein the anti-inflammatory active ingredient is 2.5 to 5.4% by weight of actin, 4 to 5% by weight of actinigen and 4.8 to 7% by weight of matyrethinol. A cosmetic composition for improving skin problems and atopic skin, comprising the mixed herbal extract according to any one of claims 1 to 4 as an active ingredient. The cosmetic composition for improving skin troubles and atopic dermatitis according to claim 5, wherein the content of the mixed herbal medicine extract contained in the cosmetic composition is 0.001 to 30% by weight based on the total weight of the cosmetic composition. Formulation of any one selected from cosmetics, essences, oils, creams, powders, packs, foundations, makeup bases, sticks which is a cosmetic or make-up products manufactured using the cosmetic composition according to claim 5 Cosmetics characterized in that it has a.