KR20090048150A - Composition against propionibacterium acnes containing caesapinia sappan l. extract and cosmetic composition containing the said composition - Google Patents

Abstract

The present invention is an anti-propionibacterium acnes containing a hydrothermal extract of joiner ( Propionibacterium) acnes ) composition and cosmetic composition for improving acne containing the same acetic acid propionibacterium acne ( Propionibacterium) acnes ) shows antimicrobial activity and suppresses the occurrence of acne.

Joiner, Staphylococcus, Acne, Propionibacterium, Antibacterial

Description

Anti-propionibacterium acnes composition containing hot water extract of joiner and composition for improving acne containing same {composition against Propionibacterium acnes containing Caesapinia sappan L. extract and cosmetic composition containing the said composition}

The present invention is an anti-propionibacterium acnes containing propeller extract ( Propionibacterium) acnes ) The composition and a cosmetic composition for improving acne containing the same.

Acne (acne vulgaris) is a skin disease common to almost all age groups, and about 80 to 95% of young people suffer from acne (L. Cordain, S. Lindeberg, M. Hurtado, K. Hill, SB). Eaton, and J. Brand-Miller, Acne vulgaris: A disease of western civilization, Arch . Dermatol . 138 (12), 1584 (2002)), especially in adolescence, are severe and suffer from clinical sequelae.

The causes of acne include, in addition to genetic and environmental factors, active sebum production due to changes in hormone secretion in the body, pore closure due to abnormal hyperkeratinization of hair follicle sebaceous glands, activation of acne bacteria and skin inflammation caused by acne bacteria. There are reactions and interactions between them. In particular, the skin inflammatory response caused by acne bacteria is a major cause of acne, and the main acne bacteria is Propionibacterium. acnes ) is known. (WJ Cunliffe, H. Gollnick, Acne; diagnosis and management, London, Dunitz Ltd. (2001))

Propionibacterium acnes ) secrete lipase and leukocyte chemotatic factors to break down the sebum enveloped in the pores narrowed by vigorous testosterone secretion into fatty acids and stimulate the hair follicle wall to induce acne and finally interact with them. Induces inflammatory reactions, forming papules, pustules, cysts, nodules and scars on the skin. (AM Layton, A review on the treatment of acne vulgaris, Int. J. Clin . Pract ., 60 (1), 64 (2006))

On the other hand, cosmetic oils, surfactants, inorganic powders, organic water-soluble polymers, and fragrances, which are commonly contained in cosmetics, can also act as a source of irritation of the skin, and can act as a nutrient medium for microorganisms to grow. It can cause acne-producing sites by causing various secondary contaminations such as contamination caused by and contamination due to careless storage.

Steroid preparations, including anti-androgens that inhibit sebum production, vitamin A derivatives such as various antibiotics and retinoic acid are widely used as an acne treatment, and antibiotics are most commonly used. However, oral antibiotics, which are prescribed over millions of cases every year, have been causing serious side effects. (P. Coates, S. Vyakrnam, EA Eady, CE Jones, JH Cove, and WJ Cunliffe, Prevalence of antibiotic-resistant propionibacteria on the skin of acne patients; 10-year surveillance data and snapshot distribution study, British J. Dermatol . , 146 , 840 (2002))

Recently, research on active ingredients that can be continuously used from natural resources including plants and has an anti-acne effect that is harmless to the human body has been actively conducted. Currently, research on anti-acne using natural extracts is being progressed or commercialized by various researchers at home and abroad using various herbaceous herbs such as licorice, magnolia, walnut tree, yellow lotus, willow, triticale, and cinnamon extract. (C. Nam, S. Kim, Y. Sim, and I. Chang, Anti-acne effects of oriental herb extracts: a novel screening method to select anti-acne agents, Skin Pharmacol . Appl . Skin Physiol ., 16 (2), 84 (2003), MT Chomnawang, S. Surassmo, VS Nukoolkarn, and W. Gritsanapan, Antimicrobial effects of Thai medicinal plants against acne-inducing bacteria, J. Ethnopharmacol . , 101 , 330 (2005), S. Higaki, S. Morimatsu, M. Morohashi, T. Yamagishi, Y. Hasegawa, Susceptibility of Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidrermidis to 10 Kampo formulations, J. Int . Med . Res . , 25 (6), 318 (1997), F. Qadan, AJ Thewaini, DA Ali, R. Afifi, A. Elkhawad, and KZ Matalka, The antimicrobial activities of Psidium guajava and Juglans regia leaf extracts to cane-developing organism, Am. J. Clin . Med. , 33 (2), 197 (2005))

Meanwhile, joiner ( Caesapinia sappan L.) is a dried deciduous arborescent or shrub belonging to Leguminosae, which is collected throughout the year to remove bark and sapwood, and to be reconstructed by taking only the central part. It is also called firewood, fire, red-eye, red porcelain, etc., and is distributed in southern China and eastern India.

The neck of the joiner contains about 2% of brazilin, a colorless primary pigment, as well as sappanin, L-phellandrene, and ocimene. Essential oils and tannins, the main constituents of which are;

Joiner has already been studied a lot, and the antimicrobial effect has been reported that the immersion solution significantly inhibits the growth of diphtheria bacteria, pneumococcus pneumoniae, type C paratyphoid bacteria, Staphylococcus aureus, hemolytic streptococci, etc. , Methanol extract is Staphylococcus aureus resistant to methicillin

( Staphylococcus aureus (MRSA)) has been reported to enhance the antimicrobial activity of ampicillin (ampicillin) and oxacillin (MRSA) against MRSA. (Kim KJ, Yu HH, Jeong SI, Cha JD, Kim SM, You YO.Inhibitory effects of Caesalpinia sappan on growth and invasion of methicillin-resistant Staphylococcus aureus. J Ethnopharmacol . 2004 Mar; 91 (1): 81-7)

The antimicrobial effect of the joiner is due to a component called brazilin, and it is reported that it inhibits the interaction of [3H] thymidine and [3H] serine in the DNA and protein synthesis of MRSA. It has been. (Hong-Xi Xu, Song F. Lee.The antibacterial principle of Caesalpina sappan. Phytother . Res . 18 , 647-651 (2004))

On the other hand, although the case of using alcohol extract as a natural preservative (Korean Patent Publication No. 10-2001-0028618) of products such as kimchi and broth, but has been disclosed, anti-propionibacterium acne ( propionibacterium) acnes ) compositions and cosmetic compositions for improving acne containing the same have not been disclosed.

Accordingly, an object of the present invention is to develop and provide an anti-acne bacterium composition based on joiner material known to have antimicrobial efficacy against some microorganisms, and a cosmetic composition for improving acne.

In order to achieve the above object, the present invention is characterized in that the anti-propionibacterium acnes ( Propionibacterium ) characterized in that it contains a hydrothermal extract of joiner acnes ) to provide a composition.

The present invention is Caesapinia Propionibacterium, characterized in that it contains a hydrothermal extract of sappan L. acnes ) composition and cosmetic composition for improving acne containing the same propionibacterium acnes ) The composition is preferably a dry powder of the joiner hot water in the form of a dry powder of 125 ㎍ / ㎖ or more in the total composition.

In addition, the extraction method commonly used in the extraction of the heat of the joiner may be used, but preferably, 10 to 20 times the weight of the joiner by distilled water is added to the joiner.

In addition, anti-propynyl'll sludge foil containing a hot water extract of the present inventors joiner Titanium arc Ness (Propionibacterium acnes ) composition can be added to the cosmetic composition for improving acne, the cosmetic composition, in addition to the hydrothermal extract of the joiner, various components generally used in cosmetics, for example, water-soluble in the range that does not reduce the effect of the present invention, if necessary It is possible to mix | blend a component, a powder component, oil, surfactant, a moisturizer, a viscosity modifier, a preservative, antioxidant, a fragrance | flavor, a pigment | dye, etc.

In addition, the cosmetic composition containing the hot-water extract of the joiner provided in the present invention may be prepared in any formulation that is commonly prepared in the cosmetic industry. For example, it can be formulated into solutions, suspensions, pastes, gels, creams, lotions, powders, soaps, oils, powder foundations, emulsion foundations, wax foundations and sprays, etc., flexible lotions, nourishing lotions, nourishing creams, It may also be prepared in the form of a massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

As described above, the hot water extract of the present inventors joiner is propionibacterium acnes ( propionibacterium acne) acnes ) and the secondary infection bacteria Staphylococcus aures ( Staphylococcus aures ) to inhibit the growth of the propionibacterium acnes ( propionibacterium acnes ) composition containing the hot water extract of the present inventors is propionibacte acne Leeum Acnes ( Propionibacterium acnes ) exhibits antimicrobial activity, containing the cosmetic composition for improving acne exhibits an effect of suppressing the occurrence of acne.

Hereinafter, the content of the present invention will be described in more detail in the following examples, but the scope of the present invention is not limited only to the following examples, and includes modifications of equivalent technical ideas.

< Example  1> Joiner Hydrothermal  Preparation of Extract

The seedlings were dried at room temperature, crushed using a grinder, powdered, and hot water extracted. 2 g of distilled water (1:20 w / v) was added to the dry weight by distilled water (1:20 w / v), followed by extraction with a water bath at 100 ° C. for 2 hours, filtered through a vacuum filter, and concentrated using a vacuum rotary concentrator. The joiner extract of% (dry weight basis) was obtained.

Experimental Example 1 Evaluation of Growth Inhibition Efficacy of Acne-Induced Strains of Joiner Hot Water Extract

Propionibacterium , the acne causing bacterium acnes ) The inhibitory effect of growth of H. pylori extract on KCTC 3314 was evaluated.

Propionibacterium acnes ) KCTC 3314 was distributed by the Korea Gene Bank and used as a culture medium, Reinforced clostridial (RC) medium.

Propionibacterium of the Propionibacterium acnes ) was activated and used to make a master plate and stored at 4 ° C. three days before the experiment, and the master plate was rebuilt every two weeks. The bacteria were inoculated in the culture medium and sealed in an anaerobic jar using a gaspack system (MERK. Germany), followed by anaerobic culture for 3 days in a 37 ° C. incubator.

Anti-acne bacterium efficacy evaluation of the seedling hydrothermal extract obtained in Example 1 was used a paper disk diffusion method. Propionibacterium acnes ) strains were incubated in Reinforce crosstridial liquid medium for 3 days, and then the bacterial suspension was diluted to a constant concentration (1.5 × 10 ⁸ CFU / ml). It was inoculated into a semi-liquid Rainforce crosstridial solid medium prepared in a 45 ° C. constant temperature water bath, mixed well, and poured into a Rainforce crosstridial solid medium. After standing for 5 to 10 minutes, when the medium was hardened, a disk (Φ 8 mm, Adventec. USA) was placed on the surface at regular intervals using tweezers. After diluting the seedling hydrothermal extract on each disc by the concentration and absorbing 50 μl and drying it sufficiently, the plate treated with the sample was dried for 3 days using a gaspack system (MERK. Germany) in an anaerobic complex. Incubated. Antimicrobial activity was measured by the size of the disk growth inhibition ring around the plate after the culture and Comparative Example 1 used triclosan (trilosan) known antimicrobial efficacy.

division Propionibacterium acnes ) KCTC 3314 Concentration (㎍ / disk) Growth inhibition ring size (mm) Comparative Example 1 25 32 50 36 100 38 Example 1 125 21 250 28 500 34

As shown in Table 1, Comparative Example 1 formed a growth inhibiting ring of 38 mm at a concentration of 100 μg / disk, and Example 1 showed an inhibitory ring of 34 mm at a concentration of 500 μg / disk. Compared with Comparative Example 1, there was a difference in concentration and potency, but considering that the joiner hydrothermal extract was a crude extract, it could be determined to exhibit excellent efficacy.

< Experimental Example  2> Joiner Hydrothermal  Evaluation of Growth Inhibitory Efficacy of Extracts Against Secondary Infection Bacteria

Staphylococcus aures , a secondary infectious bacterium that swells red and causes a severe inflammatory response, is present on the skin of the hot water extract of the presenter's joiner Growth inhibition efficacy for KCTC 1927 was evaluated.

Staphylococcus aures ) KCTC 1927 was obtained from the Korea Gene Bank and used as a culture medium, Mueller-Hinton (MH) medium. Received pre-sale Staphylococcus aureus (Staphylococcus aures ) KCTC 1927 was activated to make a master plate, and then activated at a day before the experiment while storing at 4 ° C., and the master plate was made and stored once every two weeks. The bacteria were inoculated in the culture medium, and then cultured in a 37 ° C. incubator for 18 to 24 hours.

Evaluation of the antimicrobial efficacy of the joiner hydrothermal extract prepared in Example 1 was used the same paper disk diffusion method (Experimental Example 1). Antimicrobial activity was measured by growth inhibitory ring size around the plate after completion of the culture. In Comparative Example 2, erythromycin, an antibiotic known to have antimicrobial efficacy, was used.

 division Staphylococcus aures ) KCTC 1927 Concentration (㎍ / disk) Growth inhibition ring size (mm) Comparative Example 2 62.5 12 125 14 Example 1 250 16 500 17

As shown in Table 2 above, Comparative Example 2 formed a growth inhibiting ring of 16 mm at a concentration of 250 μg / disk, and Example 1 showed a suppressing ring of 17 mm at a concentration of 500 μg / disk. It was shown to show similar efficacy at a concentration about 2 times higher than in Comparative Example 2, it was confirmed that the antimicrobial efficacy against the causative strain that can intensify the inflammation by causing secondary infections in acne.

< Experimental Example  3> Joiner Hydrothermal  Evaluation of Minimal Inhibitory Concentration of Microbial Growth of Extracts

In order to determine the effective concentration of the antimicrobial activity against acne-inducing bacteria when the joiner hydrothermal extract prepared in Example 1 was used as a cosmetic composition, the minimum growth concentration of microbial growth was evaluated using a broth dilution method.

Propionibacterium , a pre-cultured acne-inducing strain acnes ) KCTC 3314 was diluted to a constant concentration (1.5 × 10 ⁸ CFU / ml) and inoculated with 20 μl in each well containing 160 μl of medium. Joiner's hot water extract (20mg / ml of DMSO) prepared in Example 1 was subjected to two-fold serial dilution using a Reinforced CrossTrial (RC) liquid medium and inoculated with a bacterial dilution by concentration. 20 μl of each well was added and mixed well, and Comparative Example 1 used the trichromic acid used in Experimental Example 1.

96 well plates inoculated with bacterial dilutions and samples were prepared using Propionibacterium. acnes ) KCTC 3314 was anaerobicly cultured for 3 days using anaerocult Gaspack system (MERK. Germany), and the minimum concentration was determined by MIC .

division Propionibacterium acnes ) KCTC 3314 Comparative Example 1 12.5 μg / ml Example 1 125 μg / ml

As shown in Table 3, in Comparative Example 1, the growth of the bacteria hardly occurred at a concentration of 12.5 µg / ml, but in Example 1, the concentration of 125 µg / ml, which was 10 times higher, was somewhat higher. The minimum growth inhibitory concentration (MIC) was shown at. (Figure 1)

< Experimental Example  4> Joiner Hydrothermal  Growth Curve Measurement of Acne-causing Bacteria from Extracts

Propionibacterium acne-producing strain treated with Joiner hydrothermal extract prepared in Example 1 Propionibacterium acnes ) The growth curve of KCTC 3314 was determined by the incubation time and the treatment concentration of hot water extract.

Propionibacterium precultured for 3 days acnes ) After diluting the KCTC 3314 fungus suspension to a certain concentration (1.5 X 10 ⁸ CFU / ml), add 200 µl of sterile distilled water as a control to a tube inoculated with 100 µl of 5 ml of Reinforced Cross Tridial (RC) liquid medium. As a test group, the joiner hot water extract was diluted in sterile distilled water by concentration and treated in the same manner as sterile distilled water. After the reaction solution was mixed well in the tube, 200 μl of each tube was taken in a 96 well plate to make seven identical plates. Immediately after the reaction solution was transferred to a 96 well plate, the reaction time was 0 hours, and the absorbance was measured at 620 nm using an ELISA reader. Anaerobic culture of each plate was anaerobicly cultured at 37 ° C using an anaerocult gaspack system, and the absorbance was measured at 12 hours intervals.

According to the concentration of the distilled hot water extract prepared in Example 1 diluted in sterile distilled water, the growth of bacteria was measured according to the incubation time (FIG. 2), but at the concentration of 15.625 μg / ml from the untreated sample. in showed the almost similar growth curve to the control concentration of 62.5㎍ / ㎖ after cultivation for 24 hours was repeated sludge tumefaciens arc Ness (Propionibacterium acnes ) KCTC 3314 can be seen to start growing. In addition, at the concentration of 125㎍ / ㎖ effective concentration of the joiner hot water extract it can be confirmed that almost no growth occurs up to 72 hours of the final incubation time, it was confirmed that the results of Experimental Example 2 and significant results.

Therefore, the joiner hydrothermal extract of the present invention is acne bacteria Propionibacterium acnes ( Propionibacterium acnes ) Propionibacterium Inhibits the Growth of KCTC 3314 acnes ) effect was confirmed.

< Example  2> Joiner Hydrothermal  Flexible lotion with extract skin toner ) Produce

 The lotion containing the seedling hot water extract of the present invention was prepared according to a conventional method, and the composition of the lotion was shown in Table 5 below.

ingredient Content (% by weight) Comparative Example 3 Example 2 Butylene Glycol 5.0 5.0 Salicylic acid 0.5 0.5 Monolauric acid polyoxyethylene sorbitan 1.5 1.5 Polyoxyethylene lauryl ether 0.5 0.5 ethanol 15.0 15.0 Spices 0.3 0.36 antiseptic Quantity Quantity Joiner extract 0.0 One Purified water Up to 100% by weight

< Example  3> Joiner Hydrothermal  Manufacture lotion containing extract

The lotion containing the seedling hot water extract of the present invention was prepared according to a conventional method, and the composition of the lotion was shown in Table 5 below.

ingredient Content (% by weight) Sodium Ethylenediaminetetraacetic Acid 0.05 Butylene Glycol 8.0 Monooleic acid poly ethylene sorbitan 1.5 Liquid paraffin 6.0 Squalane 2.5 vaseline 1.0 Stearic acid 0.5 ethanol 10.0 Triethanolamine 0.8 Spices 0.3 antiseptic Quantity Antioxidant Quantity Joiner extract 0.25 Purified water Up to 100% by weight

< Experimental Example  4> Joiner Hydrothermal  Evaluation of Antimicrobial Activity of Lotion Containing Extracts

In order to evaluate the antimicrobial activity of the lotion prepared in Example 2, 4 ml each of the concentration in a tube propionibacterium Acnes ( Propionibacterium acnes ) After adding 1 ml of the bacterial suspension containing KCTC 3314, the tube was incubated by shaking. After 24 hours, antimicrobial activity was measured by viable cell count.

On the other hand, the antimicrobial activity was confirmed by the same method for Comparative Example 3 prepared in Example 2, the results were as shown in Table 6.

Lotion content (% by weight) Comparative Example 3 (CFU / ml) Example 2 (CFU / ml) 0.5 1 X 10 ⁵ 8.4 X 10 ² One 1 X 10 ⁵ - 2.5 7.5 X 10 ⁴ - 5 1 X 10 ³ -

The viable cell number of Comparative Example 3 was 1 × 10 ⁵ at 0.5% by weight of the lotion, whereas the viable cell number of Example 2 was 8.4 × 10 ² . In addition, when the content of the lotion is more than 1% by weight, no viable cell number was found in the lotion of Example 2, but Comparative Example 3 was almost unchanged even when the concentration of the viable cell number was 0.5% by weight.

From this, the lotion containing the extract of hot water of the present invention is propionibacterium acnes ( propionibacterium) which is the cause of acne acnes ) It was confirmed that the antimicrobial activity against KCTC 3314.

1 is a graph of microbial growth minimum inhibitory concentration (MIC) evaluation for the joiner hydrothermal extract and triclosan of the present invention.

2 is propionibacterium acnes according to the incubation time according to the concentration of the joiner hydrothermal extract of the present invention ( Propionibacterium) acnes ) is a graph of the evaluation of growth.

Claims (4)

Joiner ( Caesapinia) Propionibacterium, characterized in that it contains a hydrothermal extract of sappan L. acnes ) composition The method of claim 1, Joiner ( Caesapinia) sappan L. ) hydrothermal extract, Anti-propionibacterium acnes ( Proionibacterium ) characterized in that the dried and contained in powder form more than 125 ㎍ / ㎖ acnes ) composition The method of claim 1, The hydrothermal extract of the joiner, Anti-propionibacterium arctes ( Propionibacterium ), characterized in that extracted by adding distilled water as much as 10 to 20 times the weight of the joiner acnes ) composition Propionibacterium of claim 1 acnes ) cosmetic composition for acne improvement comprising the composition

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