KR20070080707A - Natural antiseptic substance from japanese honeysuckle and cosmetic composition containing it - Google Patents

Abstract

Provided is a natural antiseptic composition consisting of an extract of Lonicerae Flos showing excellent anti-bacterial and preservative capability without addition of an antiseptic without skin side effects, thereby being added to a cosmetic composition. The natural antiseptic composition comprises an extract of Lonicerae Flos. The cosmetic composition comprises 0.01-10 wt.% of the natural antiseptic composition consisting of the extract of Lonicerae Flos as an effective ingredient regarding a cosmetic base. The composition is in the form of skin toner, lotion, cream, foundation, essence, gel, pack, foam cleanser, skin softener, astringent, ointment, emulsion, suspension or spray.

Description

Natural preservative composed of Indongcho extract and cosmetic composition comprising same as active ingredient {NATURAL ANTISEPTIC SUBSTANCE FROM JAPANESE HONEYSUCKLE AND COSMETIC COMPOSITION CONTAINING IT}

The present invention relates to a natural preservative made of Indongcho extract and a cosmetic composition comprising the same as an active ingredient, and more particularly, it is excellent in antibacterial and antiseptic properties without adding a preservative, and can exhibit an excellent effect as a preservative. At the same time it relates to a natural preservative made of Indongcho extract that can be usefully added as a preservative in cosmetics and the like because there is no side effect on the skin and a cosmetic composition comprising the same as an active ingredient.

Most cosmetics are composed of various raw materials such as cosmetic oils, surfactants, inorganic powders, organic water-soluble polymers, and fragrances, and thus are suitable for growth of microorganisms. In addition, it is common to add chemical preservatives in order to prevent corruption caused by microorganisms due to secondary contamination from fingers and the like during use and to protect cosmetics for a long time.

As the preservatives currently used in cosmetics, paraoxybenzoic esters, phenoxyethanol, imidazolidinyl urea, DMDM hydantoin, benzalkonium chloride and the like are generally used. Recently, as new additives such as proteins, gums, vitamins, and medicinal plants are added, changes caused by the action of microorganisms are also diversified, and thus the amount of preservatives added is increased due to the weakening of preservative function.

As the amount of the preservative increases or decreases as described above, irritation and allergy symptoms are caused to the skin, thereby adversely affecting the user's skin.

Therefore, there is an urgent need for the development of a preservative having sufficient preservative power without any side effects on the skin.

In order to solve the problems of the prior art as described above, the present invention is a natural preservative made of an extract of Indongcho which can have an excellent effect as a preservative with excellent antibacterial and antiseptic properties without adding a preservative and a cosmetic composition comprising the same as an active ingredient. The purpose is to provide.

Another object of the present invention is to provide a cosmetic composition comprising a natural preservative made of Indongcho extract which can be usefully added as a preservative to cosmetics and the like without any side effects on the skin.

In order to achieve the above object, the present invention provides a natural preservative made of Indongcho extract.

In another aspect, the present invention provides a cosmetic composition comprising a natural preservative comprising the extract of Indongcho as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present inventors screened the raw materials having an antimicrobial effect, and confirmed that Indongcho extract has an effect of killing microbial strains through the CTFA Microbiology Guidelines test method, and thus completed the present invention.

The present invention is characterized by a natural preservative made of Indongcho extract and a cosmetic composition comprising the same as an active ingredient.

The dongdongcho used in the present invention (Lonicera japonica) is a semi-herbaceous shrub plant of the Capriofoliaceae native to the forests of the country's wild field, the hwahwa (인 花) and the dongdong藤, roots and stems), and the nature is cold. Its main ingredients include leucine, flavonoids, tannins, alkaloids, luteurins, inositol, saponins, roniserine, and luteolin, which are known to act on menopause, parenteral and heart, and have antipyretic and detoxifying effects. have.

The Inchocho is known to have fungal application, immune revival, anti-inflammatory, analgesic, diuretic, anti-cancer, antiviral, etc., and contains Lonicerin Luteolin-n-rhamnoglucoside as a traditional medicine for the treatment of bacterial diseases and enteritis. It has an excellent effect, heals hepatitis, jaundice, manual, and is used as an antipyretic and medicine.

The extract of Indongcho as described above may be prepared in a conventional manner, for example, 5 to 50% by weight in a conventional solvent such as distilled water, 1,3-butylene glycol, propylene glycol, or ethanol, preferably 5 to It can be prepared by extracting at 10% by weight, cooling or warming for 3 days, and then filtering with a 0.1-1 μm filter.

In another aspect, the present invention provides a cosmetic composition comprising a natural preservative composed of the above Inchocho extract as an active ingredient, the cosmetic composition comprises a natural preservative consisting of the extract of Indongcho in a conventional cosmetic base as 0.01 to 10% by weight It is desirable to be. When the natural preservative comprising the extract of Indongcho is contained in the cosmetic base less than 0.01% by weight, there is a problem that it is difficult to expect a substantial antiseptic effect, when it exceeds 10% by weight it is difficult to mask the specific odor of the extract of Indongcho There is a problem.

As described above, the cosmetic composition of the present invention containing a natural preservative composed of Indongcho extract as an active ingredient may further include an oil component, a dispersant, a fatty alcohol, or a sunscreen used in a conventional cosmetic composition in addition to sugar alcohols. If necessary, additives such as fragrances, preservatives, and surfactants that are applied to conventional cosmetic compositions may, of course, be further included.

In addition, the cosmetic composition of the present invention is used for skin, such as skin, lotion, cream, foundation, essence, gel, pack, foam cleansing, softening lotion, astringent makeup, foundation, ointment, emulsion, suspension, or spray And may include a variety of conventional carriers and additives suitable for each of these formulations and well known in the art.

Cosmetic composition comprising a natural preservative as an active ingredient consisting of the extract of Indongcho of the present invention as an active ingredient has excellent antimicrobial, antiseptic, and preservative for a long time, at the same time there is no side effect when used on the skin.

Hereinafter, preferred examples are provided to help understanding of the present invention, but the following examples are merely to illustrate the present invention, and the scope of the present invention is not limited to the following examples.

EXAMPLE

Preparation example. Indongcho Extract Manufacturer

Phosphorous vinegar was extracted with 30% by weight in a solvent in which distilled water: 1,3-butylene glycol: ethanol was mixed at 6: 2: 2, respectively, and cooled for 3 days, and then filtered through a 0.2-0.5 μm filter so that no precipitate formed. To obtain an extract of Indongcho.

Using the obtained Indongcho extract, Staphylococcus aureus (Staphylococcus aureus, ATCC 6538), Escherichia coli (ATCC 10536), Pseudomonas aeruginosa (Pseudomonas aeruginosa, ATCC 15522) ), Antimicrobial effects against Propionibacterium acnes (ATCC 6919), Candida albicans (ATCC 10231), and Aspergillus niger (ATCC 9642).

First, the strains of Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 10536), and Pseudomonas aeruginosa (ATCC 15522) were brain heart infusions. incubation) was used to incubate at 35 ° C. for 24 hours. 100 μl of a pre-prepared and sterilized brain heart infusion liquid medium was put into each well of a 96-well, U-form micro plate (Falcon, USA). Here, the prepared Indongcho extract was diluted step by step, inoculated to 10 ^{5-10 6} CFU / mL, and the treated 96-well, U-form micro plates were incubated in a 37 ° C. incubator for 24 hours. .

In addition, the propionibacterium exeni (Propionibacterium acnes, ATCC 6919) strain was pre-cultured for 48 hours in an anaerobic incubator at 35 ℃ using Brain Hert infusion liquid medium. Then, the sterilized brain heart infusion liquid medium was put in 100 μL each well of 96-well, U-form micro plate. Herein, the prepared Indongcho extract was diluted step by step, inoculated to 10 ^{5-10 6} CFU / mL, and the treated 96-well, U-form micro plate was incubated in an incubator at 30 ° C. for 48 hours. It was.

Candida albicans (ATCC 10231) strains were preincubated for 48 hours at 30 ° C. using sabouraud dextrose liquid medium. Subsequently, 100 μl of each well of a pre-prepared and sterilized savored dextrose liquid medium in 96-well and U-form micro plates was added thereto. Here, the prepared Indongcho extract was diluted step by step, inoculated to 10 ⁵ CFU / mL, and then treated 96-well, U-form micro plate was incubated for 48 hours in an incubator at 30 ℃.

Aspergillus niger (ATCC 9642) strains were incubated at 30 ° C. for 5 days using a savoraoud dextrose solid medium. Subsequently, 100 μl of each well of a pre-prepared and sterilized savored dextrose liquid medium in 96-well and U-form micro plates was added thereto. Here, the prepared Indongcho extract was diluted step by step, inoculated to 10 ⁵ CFU / mL, and then treated 96-well, U-form micro plate was incubated for 5 days in an incubator at 30 ℃.

Through the above experimental results, the minimum inhibitory concentration (MIC) of Indongcho extract for each strain is shown in Table 1 below. At this time, by visually confirming the wells in each well, the lowest concentration of the sample without bacterial growth was determined by MIC. In the case of Aspergillus Niger, after incubating for 5 days, the lowest concentration of no mycelium was formed on the surface of the medium. Determined by MIC.

division Minimum Inhibitory Concentration (MIC) of Indongcho Extract Staphylococcus aureus (ATCC 6538) 1.15%  Escherichia coli (ATCC 10536) 0.625% Pseudomonas aeruginosa (ATCC 15522) 0.9375% Propionibacterium acnes (ATCC 6919) 1.15% Candida albicans (ATCC 10231) 0.9375% Aspergillus niger (ATCC 9642) 0.625%

As shown in Table 1, it was confirmed that the extract of Indongcho constituting the natural preservative used in the cosmetic preservative of the present invention exhibits a very good antimicrobial activity against a wide range of strains.

Examples 1-3 and Comparative Example 1

The skin was prepared according to a conventional method with the ingredients and compositions shown in Table 2 below. The unit in Table 2 below is weight percent.

division Example 1 Example 2 Example 3 Comparative Example 1 Ethanol phase ethanol 5.0 5.0 5.0 5.0 Polyoxyethylene Cured Castor Oil 0.2 0.2 0.2 0.2 Awards Purified water Up to 100% by weight Butylene glycol 3.0 3.0 3.0 3.0 Carbomer 0.05 0.05 0.05 0.05 additive incense Quantity Quantity Quantity Quantity antiseptic Methylparaben - - - 0.15 Ethylparaben - - - 0.02 Propylparaben - - - 0.02 Indongcho Extract 0.5 1.0 2.0 -

After each 40 g of the skin prepared in Examples 1 to 3 and Comparative Example 1, Staphylococcus aureus (ATCC 6538), Pseudomonas aeruginosa (ATCC 15522), And mixed bacteria such as Escherichia coli (ATCC 10536) were added and mixed so as to have 10 ⁶ grains per g of skin, and then stored for 4 weeks. Then, the results of the antiseptic force measured and checked every 1 day, 7 days, 14 days, 21 days, 28 days from the day of inoculation of the change of the number of inoculated bacteria are shown in Table 3 below.

The unit of Table 3 below is CFU / g.

division Example 1 Example 2 Example 3 Comparative Example 1 Inoculation bacteria 10 ⁶ 10 ⁶ 10 ⁶ 10 ⁶ 1 day later 2.5 × 10 ⁵ <20 <20 4.4 × 10 ⁴ 7 days later <20 <20 <20 10 ² 14 days later <20 <20 <20 <20 21 days later <20 <20 <20 <20 After 28 days <20 <20 <20 <20

After 40 g of the skins prepared in Examples 1 to 3 and Comparative Example 1, respectively, Aspergillus niger (ATCC 8642) and yeast Candida albicans (ATCC 10231) After adding and mixing up to 10 ⁵ grains per skin, check the measured preservation results every 1 day, 7 days, 14 days, 21 days and 28 days from the day of inoculation. Table 4 shows.

The unit of Table 4 below is CFU / g.

division Example 1 Example 2 Example 3 Comparative Example 1 Inoculation bacteria 10 ⁵ 10 ⁵ 10 ⁵ 10 ⁵ 1 day later 8.3 × 10 ³ 20 <20 1.3 × 10 ³ 7 days later <20 <20 <20 20 14 days later <20 <20 <20 <20 21 days later <20 <20 <20 <20 After 28 days <20 <20 <20 <20

As shown in Table 3 and Table 4, according to the present invention, the cosmetics of the examples containing a lead preservative composed of Indongcho extract according to the present invention show comparable or higher antiseptic power compared to the cosmetics of the comparative example containing a conventional synthetic preservative. Could confirm.

Examples 4-6 and Comparative Example 2

The lotion was prepared according to a conventional method with the ingredients and compositions shown in Table 5 below. The units in Table 5 below are by weight.

division Example 4 Example 5 Example 6 Comparative Example 2 Awards Purified water Up to 100% by weight Propylene glycol 2.0 2.0 2.0 2.0 Butylene glycol 5.0 5.0 5.0 5.0 Carbomer 0.12 0.12 0.12 0.12 Paid Cetostearyl alcohol 0.8 0.8 0.8 0.8 Self-emulsifying monostearic acid 1.0 1.0 1.0 1.0 Stearic acid 0.5 0.5 0.5 0.5 Liquid paraffin 0.5 0.5 0.5 0.5 Squalane 5.0 5.0 5.0 5.0 Isocetyloctanoate 2.0 2.0 2.0 2.0 Dimethylsiloxane 0.3 0.3 0.3 0.3 Sorbitan monosulfate 0.5 0.5 0.5 0.5 Monostearate Polyethylene Glycol 1.2 1.2 1.2 1.2 additive incense Quantity Quantity Quantity Quantity antiseptic Methylparaben - - - 0.2 Ethylparaben - - - 0.1 Propylparaben - - - 0.1 Indongcho Autumn Extract 1.0 2.0 3.0 -

Staphylococcus aureus (ATCC 6538), Pseudomonas aeruginosa (ATCC 15522), in the same manner as described above using the lotions prepared in Examples 4 to 6 and Comparative Example 2, And antiseptic ability against mixed bacteria such as Escherichia coli (ATCC 10536) and mold Aspergillus niger (ATCC 8642) and yeast Candida albicans (ATCC 10231). Antiseptic force was measured and the results are shown in Tables 6 and 7 below.

At this time, the units of Tables 6 and 7 is CFU / g.

division Example 4 Example 5 Example 6 Comparative Example 2 Inoculation bacteria 10 ⁶ 10 ⁶ 10 ⁶ 10 ⁶ 1 day later 5.5 × 10 ⁵ <20 <20 2.3 × 10 ⁵ 7 days later <20 <20 <20 50 14 days later <20 <20 <20 <20 21 days later <20 <20 <20 <20 After 28 days <20 <20 <20 <20

division Example 4 Example 5 Example 6 Comparative Example 2 Inoculation bacteria 10 ⁵ 10 ⁵ 10 ⁵ 10 ⁵ 1 day later 5.5 × 10 ⁴ 10 ⁴ 2.3 × 10 ³ 6.3 × 10 ⁵ 7 days later 10 ⁴ 10 ³ <20 20 14 days later 10 ⁴ <20 <20 <20 21 days later 1.2 × 10 ² <20 <20 <20 After 28 days 50 <20 <20 <20

As shown in Table 6 and Table 7, the cosmetics of the embodiments containing a lead preservative consisting of the extract of Indongcho according to the present invention exhibits the same or more antiseptic force compared to the cosmetic of the comparative example containing a conventional synthetic preservative Could confirm.

Examples 7-9 and Comparative Example 3

To prepare a cream according to the conventional method with the ingredients and compositions shown in Table 8. The units in Table 8 below are by weight.

division Example 7 Example 8 Example 9 Comparative Example 3 Awards Purified water Up to 100% by weight glycerin 2.0 2.0 2.0 2.0 Butylene glycol 5.0 5.0 5.0 5.0 Propylene glycol 3.0 3.0 3.0 3.0 Xanthan Gum 0.2 0.2 0.2 0.2 Paid Cetostearyl alcohol 3.0 3.0 3.0 3.0 Self-emulsifying monostearic acid 1.5 1.5 1.5 1.5 Lipophilic monostearic acid 1.5 1.5 1.5 1.5 Beeswax 0.5 0.5 0.5 0.5 Liquid paraffin 8.0 8.0 8.0 8.0 Squalane 7.0 7.0 7.0 7.0 Isocetyloctanoate 4.0 4.0 4.0 4.0 Purified jojoba oil 4.0 4.0 4.0 4.0 Dimethylsiloxane 0.3 0.3 0.3 0.3 Sorbitan monosulfate 1.0 1.0 1.0 1.0 Monostearic Acid Polyethylene Glycol 1.2 1.2 1.2 1.2 additive incense Quantity Quantity Quantity Quantity antiseptic Methylparaben - - - 0.2 Ethylparaben - - - 0.1 Propylparaben - - - 0.1 Indongcho Extract 1.0 2.0 3.0 -

Staphylococcus aureus (ATCC 6538), Pseudomonas aeruginosa (ATCC 15522), in the same manner as described above using the cream prepared in Examples 7 to 9 and Comparative Example 3, And antiseptic ability against mixed bacteria such as Escherichia coli (ATCC 10536) and mold Aspergillus niger (ATCC 8642) and yeast Candida albicans (ATCC 10231). Antiseptic force was measured, and the results are shown in Tables 9 and 10 below.

At this time, the units of Table 9 and 10 is CFU / g.

division Example 7 Example 8 Example 9 Comparative Example 3 Inoculation bacteria 10 ⁶ 10 ⁶ 10 ⁶ 10 ⁶ 1 day later 5.5 × 10 ⁴ <20 <20 2 × 10 ⁵ 7 days later <20 <20 <20 30 14 days later <20 <20 <20 <20 21 days later <20 <20 <20 <20 After 28 days <20 <20 <20 <20

division Example 7 Example 8 Example 9 Comparative Example 3 Inoculation bacteria 10 ⁵ 10 ⁵ 10 ⁵ 10 ⁵ 1 day later 7.8 × 10 ⁴ <20 <20 10 ⁵ 7 days later 2.1 × 10 ² <20 <20 5 × 10 ³ 14 days later <20 <20 <20 10 ² 21 days later <20 <20 <20 90 After 28 days <20 <20 <20 <20

As shown in Table 9 and Table 10, according to the present invention, the cosmetics of the embodiments containing a lead preservative composed of Indongcho extract according to the present invention exhibits the same or higher antiseptic power as compared to the cosmetic of the comparative example containing a conventional synthetic preservative. Could confirm.

Examples 10-12 and Comparative Example 4

To prepare a foundation according to a conventional method with the components and compositions shown in Table 11. The units in Table 11 below are by weight.

division Example 10 Example 11 Example 12 Comparative Example 4 Awards Purified water Up to 100% by weight Propylene glycol 2.0 2.0 2.0 2.0 Butylene glycol 5.0 5.0 5.0 5.0 Carbomer 0.2 0.2 0.2 0.2 Paid Stearic acid 1.0 1.0 1.0 1.0 Triethanolamine 1.0 1.0 1.0 1.0 Ferric oxide 0.3 0.3 0.3 0.3 Yellow iron oxide 1.8 1.8 1.8 1.8 Black iron oxide 0.1 0.1 0.1 0.1 Titanium dioxide 10.0 10.0 10.0 10.0 Silica 5.0 5.0 5.0 5.0 Cetostearyl alcohol 1.5 1.5 1.5 1.5 Monostearic acid 0.2 0.2 0.2 0.2 Polyoxyethylene sorbitan 0.3 0.3 0.3 0.3 Sesquioleate sorbitan 2.0 2.0 2.0 2.0 Stearic acid 0.1 0.1 0.1 0.1 Paraoxybenzoic acid propyl 4.0 4.0 4.0 4.0 Octyldodecanol 4.0 4.0 4.0 4.0 Squalane 5.0 5.0 5.0 5.0 Isostearyl isostearate 0.5 0.5 0.5 0.5 Methylpolysiloxane 0.5 0.5 0.5 0.5 additive incense Quantity Quantity Quantity Quantity antiseptic Methylparaben - - - 0.2 Ethylparaben - - - 0.1 Propylparaben - - - 0.1 Indongcho Extract 1.0 2.0 3.0 -

Staphylococcus aureus (ATCC 6538), Pseudomonas aeruginosa (ATCC 15522), in the same manner as described above using the foundation prepared in Examples 10 to 12 and Comparative Example 4, And antiseptic ability against mixed bacteria such as Escherichia coli (ATCC 10536) and mold Aspergillus niger (ATCC 8642) and yeast Candida albicans (ATCC 10231). Antiseptic force was measured and the results are shown in Tables 12 and 13 below.

At this time, the unit of Table 12 and 13 is CFU / g.

division Example 10 Example 11 Example 12 Comparative Example 4 Inoculation bacteria 10 ⁶ 10 ⁶ 10 ⁶ 10 ⁶ 1 day later 10 ⁴ <20 <20 10 ⁵ 7 days later <20 <20 <20 10 ² 14 days later <20 <20 <20 <20 21 days later <20 <20 <20 After 28 days <20 <20 <20 <20

division Example 10 Example 11 Example 12 Comparative Example 4 Inoculation bacteria 10 ⁵ 10 ⁵ 10 ⁵ 10 ⁵ 1 day later 7.1 × 10 ⁴ 10 ³ <20 10 ⁵ 7 days later <20 <20 <20 8 × 10 ³ 14 days later <20 <20 <20 10 ³ 21 days later <20 <20 <20 7.1 × 10 ² After 28 days <20 <20 <20 <20

As shown in Table 12 and Table 13, according to the present invention, the cosmetics of the examples containing a lead preservative made of Indongcho extract according to the present invention show comparable or higher antiseptic power as compared to the cosmetic of the comparative example containing a conventional synthetic preservative. Could confirm.

In addition, to evaluate the safety and stimulation for the human body using the cosmetics prepared above, a primary stimulation test was performed on the human body through a human patch test as follows.

First, 40 healthy men and women were tested according to CTFA guidelines (see The Cosmetic, Toility and Fragrance Association. Inc. Washington, D.C., 20036, 1991). 15 μl of the skins of Example 3 and Comparative Example 1 were added dropwise to the Fin chamber, and it was fixed on the back of a human body as a test site. Subsequently, after patching for 24 hours, the patch was removed and after 4 hours, the skin reaction of the test site was determined according to the following criteria and equations, and the results are shown in Table 14 below.

Criteria - No erythema or unusual symptoms ± Slightly redder than the surroundings + Significantly redder than the surroundings ++ Severe redness and swelling than around

[Equation]

Stimulus = [{(±) number × 1} + {(+) number × 2} + {(++} number × 3}] / number of subjects

division Judgment result Stimulus ++ + ± - Example 3 0 0 2 38 0.05 Comparative Example 1 0 One 6 33 0.20

As shown in Table 14, according to the present invention it was confirmed that the cosmetic of Example 3 containing a lead preservative consisting of Indongchocho extract significantly reduced skin irritation compared to the cosmetic of the comparative example containing a conventional synthetic preservative.

According to the present invention, it is excellent in antimicrobial and antiseptic properties without adding a preservative, and can provide excellent effects as a preservative, and has excellent preservative power for a long time, and has no side effects on the skin, and thus can be usefully added as a preservative to cosmetics. It can be effective.

Although only described in detail with respect to the described embodiments of the present invention, it will be apparent to those skilled in the art that various modifications and variations are possible within the technical spirit of the present invention, it is natural that such variations and modifications belong to the appended claims. .

Claims (4)

Natural preservative consisting of Indongcho extract. A cosmetic composition comprising a natural preservative comprising the extract of Indongcho according to claim 1 as an active ingredient. The method of claim 2, Cosmetic composition, characterized in that the natural preservative consisting of the extract of Indongcho is contained in the cosmetic base 0.01 to 10% by weight. The method of claim 2, The cosmetic composition is a formulation selected from the group consisting of skin, lotion, cream, foundation, essence, gel, pack, foam cleansing, softening, converging cosmetics, foundation, ointment, emulsion, suspension, and spray Cosmetic composition.