KR20090014520A - Composition for mouth washing and deodoring including natural inorganic minerals of deep sea water - Google Patents

Abstract

A composition is provided to gain superior antibacterial effect and excellent odor removal effect. A mouth detergent made of natural inorganic minerals in the sea includes deep sea water. A gradient of the deep sea water is 100-13000. The deep sea water is comprised of one kind of the deep sea water or mixing deep sea water which is mixed with over two kinds water selected from a group consisting of raw water, enriched water, permeated water and purified water. A deodorizing agent includes a deodorant compound. The deodorizing agent is selected from a group consisting of hydrogen peroxide of 0.001-0.05wt, titanium oxide of 0.01-0.5wt, and their mixture.

Description

Composition for mouth washing and deodoring including natural inorganic minerals of deep sea water}

The present invention relates to mouthwashes and deodorants, and more particularly, to mouthwashes and deodorants containing deep sea water as an active ingredient.

Deep sea water refers to seawater collected at a depth of 200 m or less without sunlight reaching, and does not mix with each other like water and oil because there is a temperature difference of more than 20 ° C from surface water. The deep sea water flowing through the deep sea has low temperature stability due to little change in water temperature throughout the year, and also contains inorganic nutrients because photosynthesis does not occur because sunlight does not reach, and especially the essential trace elements such as magnesium and calcium are balanced with various minerals. Included. In addition, since deep sea water is not contaminated with artificial materials, and there are few organic substances or pathogens to be decomposed, there is an advantage of less bacterial propagation. Therefore, in many countries, various studies have been made for removing sodium chloride (NaCl) from deep sea water and then using it for beverages, food, and cosmetics.

On the other hand, due to the rapid urbanization and population increase due to economic development, transportation and household waste have increased rapidly, and environmental pollution is a serious problem. For example, because it is not easy to ventilate indoor air due to air pollution, it is difficult to remove odors or various odors caused by various factors in food production in a closed living space. Bacteria and fungi that cause the back will multiply. Currently, in order to remove odors generated in indoor spaces, a method of absorbing odors using materials such as activated carbon or zeolite or a masking method using aromatic substances to reduce odors are used. They not only have a very low odor removal effect, but also cause headaches, and after a long time, the collected bad smell substances are released, the bad smell is generated again.

Economic development has also brought about changes in the diet patterns of modern people, which has resulted in the development of hundreds of aerobic microorganisms, such as bacterial fungi, in the oral cavity and teeth. . Bad breath originates from sulfur-containing amino acids cysteine, methionine, or proteins containing them, or volatile compounds formed from peptides. That is, most nonglycolytic bacteria present in the oral cavity decompose cysteine to form hydrogen sulfide, and decompose methionine to methyl mercaptane, both of which are the culprit of bad breath. As people's awareness of oral cleanliness increases, there is an increasing tendency to promote oral hygiene using toothpastes that are easy to carry and can be used anywhere and anytime. The market for oral cleaning agents has been expanding since its launch in 1982. However, most commercial mouthwashes contain various chemicals such as benzethonium chloride, an antibacterial effect, and alcohol and glycerin, stabilizers, preservatives, and beards, which have antibacterial effects. Swallowing large amounts during the washing process can cause side effects such as vomiting, nervous breakdowns and diarrhea. As such, all currently available products contain large amounts of chemicals, and thus do not improve the safety, nontoxic and non-irritant effects on the human body. By improving the various problems, it can be manufactured at a low price, excellent antibacterial or odor removing effect, does not cause environmental pollution, is not only safe for the human body, but also the development of oral cleaning agents containing natural products as an active ingredient It is requested.

Accordingly, it is an object of the present invention to provide an oral cleanser and a deodorant which are harmless to the human body and excellent in antibacterial and odor removing effects.

Another object of the present invention is to provide a mouthwash and deodorant containing natural products as an active ingredient.

In order to achieve the above object, the present invention provides a mouthwash and deodorant containing deep sea water as an active ingredient. Here, the deep sea water of hardness is 100 to 13,000, the deep sea water having a hardness of 100 to 13,000 is prepared by mixing deep water alone or two or more selected from the group consisting of raw water, concentrated water, permeate and purified water. desirable. In addition, the deodorant preferably further comprises a deodorant compound selected from the group consisting of hydrogen peroxide (H ₂ O ₂ ), titanium oxide (TiO ₂ ) and mixtures thereof.

Hereinafter, the present invention will be described in more detail.

Deep sea water contained in the mouthwash and deodorant according to the present invention, serves as an active ingredient to perform the mouthwash and deodorant function, and serves as a solvent for dissolving other components of the mouthwash and deodorant. As the deep sea water, raw water, which is the deep sea water taken out, concentrated water in which the ionic components of the raw water are concentrated, permeated water in which the ionic components are reduced from the raw water, a mixture thereof, and the like may be used. For example, deep sea water having a hardness of 100 to 13,000, more preferably 200 to 10,000, most preferably 300 to 5,000 may be used. If the hardness of the deep sea water is too low, there is a fear that a sufficient oral cleaning effect may not be obtained, and if the hardness is too high, there is a fear that economic feasibility to industrialize in reality. As the concentrated water and the permeated water, concentrated water and permeated water generated while removing sodium chloride from raw water may be used by conventional methods such as reverse osmosis and electrodialysis. The depth of the deep sea water may include the raw water, It can be adjusted by appropriately mixing two or more kinds selected from the group consisting of concentrated water, permeated water and purified water. In addition, in the deep sea water, the content of sodium ions may be reduced to 50 parts by weight or less, preferably 5 to 30 parts by weight or less with respect to 100 parts by weight of magnesium ions. It is desirable because it can remove the salty taste of deep sea water. Deep sea water having a reduced content of sodium can be prepared by known methods, for example, by the method described in the applicant's patent application 10-2006-0054899.

For the raw water, permeated water and concentrated water, the antimicrobial and methyl mercaptain removal rates of general pathogens and oral pathogens were examined. As a result, when using raw water and concentrated water containing a large amount of sodium chloride, potassium, magnesium, When a large amount of minerals such as calcium, chlorine, copper, zinc and the like are used, and mixed water having a hardness of 100 to 13,000 is used, the survival rate of oral bacteria is low and odor inhibiting activity is high. Therefore, the oral cleanser of the present invention containing the deep sea water as a main component is very excellent in the removal effect of the odor causing substance methyl mercaptain, and also excellent antibacterial activity against Streptococcus Mutans, a representative tooth decay bacteria. In addition, the antimicrobial, deodorant and human stability are superior to conventional commercial products.

In addition, the deodorizing agent according to the present invention may further include a deodorizing compound such as hydrogen peroxide (H ₂ O ₂ ), titanium oxide (TiO ₂ ), a mixture thereof. When the hydrogen peroxide (H ₂ O ₂ ) is used, the content is 0 to 0.05 parts by weight, preferably 0.001 to 0.05 parts by weight with respect to 100 parts by weight of deep sea water, the content of titanium oxide (TiO ₂ ), if used Is 0 to 0.5 parts by weight, preferably 0.01 to 0.5 parts by weight, relative to 100 parts by weight of deep sea water. When neither of the two substances is included, the deodorizing effect of the deodorant of the present invention may be partially reduced. However, when one or more of the two substances is included, deep ocean water having antimicrobial activity and odor removal effect by itself is produced. Since it is included as a main solvent, it shows an excellent deodorizing effect compared to the conventional product.

The mouthwash and deodorant according to the present invention may further include various blending components, if necessary, within the scope of not impairing the purpose and effect of the composition. First, in the case of an oral cleaning agent, examples of the compounding component include sodium fluoride, a dissolution aid, a surfactant, a sweetener, a flavoring agent, a coloring agent, and the like. In consideration of the feeling of use and safety, the oral cleaning agent is preferably maintained at pH 5.5 ~ 7.0. The sodium fluoride is a substance known to have antibacterial activity and bacterial activity, and may be included in an amount of about 0.01 to 0.04 parts by weight based on 100 parts by weight of deep sea water. At this time, when the content of the sodium fluoride exceeds 0.04 parts by weight, there is a problem that the cytotoxicity is excessively strong. On the other hand, in the case of a typical mouthwash using purified water as the main solvent, when the sodium fluoride content is less than 0.01 parts by weight with respect to 100 parts by weight of the solvent, the antibacterial and odor inhibitory effects are significantly lowered, whereas the composition of the present invention has its own antibacterial activity. And since the deep sea water having an odor removal effect as a main solvent, even if the content of sodium fluoride is less than 0.01 parts by weight, there is little decrease in the deodorizing effect. The dissolution aid is included in about 0.01 to 15 parts by weight based on 100 parts by weight of deep sea water. The dissolution aid is to dissolve it when additionally insoluble in the deep sea water in the preparation of oral cleansers, there is an alcoholic compound to add a moisturizing glycerin or sorbitol, or a refreshing feeling. The surfactant is included from 0.05 to 0.15 parts by weight with respect to 100 parts by weight of deep sea water, if the content of the surfactant is less than 0.05 parts by weight, the wetting agent, etc. may be separated without dissolving in the antimicrobial composition, If the content exceeds 0.15 parts by weight, it is only economically disadvantageous without any particular benefit. The surfactant is used to dissolve glycerin, sorbitol, and the like as a humectant. For example, polyoxyl 40 hydrogenated castor oil, twin 80, triton S-100, sodium lauryl sulfate, and the like are used.

Oral cleaning agents and deodorants of the present invention may further include sweetening agents, complexing agents, flavoring agents, coloring agents and the like, in order to further emphasize the aesthetic function, flavor and the like as necessary. As the sweetener, saccharin sodium, xylitol, etc., which have a strong sweet taste in a small amount, may be used, and may be used in an amount of 0 to 0.1 parts by weight based on 100 parts by weight of deep sea water. The flavoring agent may be used as a natural flavor mint, it is included in 0 to 0.2 parts by weight based on 100 parts by weight of deep sea water. As the colorant, blue No. 1 may be used, and the like may be included in an amount of 0 to 0.1 parts by weight based on 100 parts by weight of deep sea water.

The mouthwash and deodorant according to the present invention can reduce the content of chemical components such as sodium fluoride, dissolution aids, surfactants, etc., and thus have improved stability compared to conventional products containing a large amount of harmful chemical components. In addition, the deep sea water, which is the main solvent of the mouthwash and deodorant of the present invention, contains a large number of metal ions, for example, silver, copper, zinc, aluminum, iron, nickel, and chromium, which are known to have an antibacterial effect. . The metal ions migrate into the microbial cells in a state dissolved in water, and then combine with the -SH group of the enzyme present in these cells to lower the activity of the enzyme. As a result, the growth of the microorganisms is suppressed or killed, resulting in odor. Removed. This effect is more active when the affinity of microbial cells for metal ions is large. The mouthwash and deodorant according to the present invention can be used by applying a suitable amount by a conventional method such as gargle, spraying, coating, deposition.

As described above, the oral cleanser and the deodorant according to the present invention is a marine natural inorganic mineral oral clean and deodorant composition, by including the deep sea water as an active ingredient, there is an advantage of superior antibacterial, deodorant effect and safety than conventional products.

Hereinafter, the present invention will be described in more detail with reference to Examples. The following examples illustrate the present invention and the present invention is not limited by the following examples.

Preparation Example 1 Deep Sea Water Collection and Purification

The deep seawater collected at a depth of 500 m in the East Sea of Korea is finely filtered with a micro filter (material: Polytetrafluoroethylene (PTFE), pore size: about 0.5 µm, manufactured by Saehan Co., Ltd.) to remove impurities, and then reverse osmosis system (product of Dow Chemical Company, FILMTEC). , SW30-4021) was separated into reverse osmosis concentrated water and reverse osmosis filtered water. In addition, the filtered deep sea water (raw water), the electrodialysis apparatus (MICRO ACILYZER G4SK, anion membrane: NEOSEPTA AMX SB, cationic membrane: ACIPLEX K-192 / ASAHI KASEI CORPORATION, JAPAN, operating conditions: current density 2,5A / m ² , STACK voltage 38V constant voltage operation / circulating flow rate 13.1L / min) was separated into electrodialysis concentrated water and electrodialysis filtered water. The content of minerals in the deep sea water (raw water), concentrated water and filtered water is shown in Table 1 (ND: Not detected).

Element (ppm) enemy Reverse Osmosis Filtrate Reverse Osmosis Concentrated Water Electrodialysis filtrate Electrodialysis concentrate Al 0.28 0.004 0.56 0.008 0.57 As ND ND ND 0.002 0.002 Ba ND ND ND ND ND Ca 419.0 0.006 687.9 9.7 713.7 CD ND ND ND ND ND Co 0.52 ND 1.6 ND 2.0 Cr 1.9 0.006 0.026 1.9 3.9 Cu 0.053 0.004 2.4 0.007 1.7 Fe 0.005 0.001 1.6 ND 1.5 Mg 1285 0.04 2586 118 2258 Mn 0.043 ND 0.053 ND 0.061 Pb <0.001 <0.001 <0.001 ND ND Sr 6.61 ND ND 0.46 1.65 K 430 5.7 560 1.78 461 Zn 0.01 0.002 0.23 0.002 0.31 Hardness 6316 0.18 12,322 507 11,042

Production Example  2. Manufacturing of Deep Sea Water According to Hardness

The water produced in Preparation Example 1 was mixed to prepare deep water of hardness 200, 500 and 1000 containing minerals. At this time, the hardness is 50 ml of mixed deep water in a beaker, 1 ml of ammonium chloride-ammonia buffer solution of pH 10 and 1-2 drops of EBT indicator (eriochrome black T indicator), followed by 0.01M ethylenediaminetetraacetic acid (EDTA 15.5 ml was added dropwise. Before titration, the reaction color is red, but when the EDTA solution is added dropwise, the color is completely blue. The main components of the deep sea water according to the hardness thus produced are shown in Table 2 below.

ingredient Hardness (mg / L): 250 Hardness (mg / L): 500 Hardness (mg / L): 1000 K 14.87 29.48 58.71 Na 45.92 91.42 182.43 Mg 51.31 102.58 205.14 Ca 15.47 30.92 61.83 Cl 53.69 106.18 211.15 SO4 8.94 17.88 35.77 B 0.38 0.52 0.80 Cu 0.42 0.61 0.88 Zn 1.44 1.98 2.41 Total content 192.44 381.57 759.12

Example  1. Preparation of mouthwash

The primary concentrated water of Preparation Example 1 and sodium fluoride, dissolution aids, surfactants, sweeteners, complexing agents, flavoring agents and colorants were mixed in an amount as shown in Table 3 to prepare an oral cleanser.

Example  2. Preparation of mouthwash

As a deep sea water, instead of using the first concentrated water, except for using the permeated water of Preparation Example 1, by mixing each component in the same ratio as in Example 1 (Example 2-1 to practice Example 2-4) was prepared.

Example  3. Preparation of mouthwash

As a deep sea water, instead of using the first concentrated water, except for using the deep water of hardness 250 obtained in Preparation Example 2, by mixing each component in the same ratio as in Example 1 above (Example 3- 1 to Example 3-4) were prepared.

Comparative Example 1. Preparation of mouthwash

The composition (content per 100 mL of mouthwash) of Gagrin (Dong-A Pharmaceutical Co., Ltd.), which is a currently available mouthwash, is shown in Table 4 below.

Experimental Example  1. deep ocean waters methyl Mercaptain  Removal rate measurement

(1-1) 2 mL of methyl mercaptain standard solution (1 μg / μL in benzene, Wako pure Chem., Osaka, Japan) is dissolved in 10 mL ethanol solution, diluted 10-fold with 40% ethanol solution, and placed in 5 mL vial. Frozen at -70 ° C.

(1-2) 4 ml of deep sea water of Preparation Examples 1 and 2 were placed in a 50 ml vial, the methyl mercaptain sample of (1-1) was dissolved, and then 1 mL was taken and added to the vial. The vial was immediately sealed with a silicone cap, then stirred for 5 seconds using a vortex mixer (Vision Science) and incubated at 37 ° C. for 6 minutes. Subsequently, 50 μg of methyl mercaptain liberated on top of the vial was taken and analyzed by GC / MSD (shimadzu, Japan), and the results are shown in Table 5 below. At this time, the bad breath inhibitory activity was calculated by the following equation.

x 100Bad breath inhibitory activity (%) =

x 100

C: metal mercaptain peak area of the control

S: Methyl mercaptain peak area at the time of sample addition

Types of deep ocean water Methyl mercaptain removal rate (%) enemy 37.2 Concentrated water 44.5 Permeate 20.6 Depth of Hardness 250 28.7 Depth of Hardness 500 30.2 Depth of Hardness 1000 34.8

Experimental Example  2. Oral cleaning agent methyl Mercaptain  Removal rate measurement

Instead of using deep sea water, except for using the oral cleanser prepared in Examples 1 to 3 and Comparative Example 1, in the same manner as in Experimental Example 1-2, by measuring the methyl mercaptain removal rate Table 6 Shown in

Experimental Example  3. Oral Bacteria in Ocean Deep Water Treatment Survival rate  Measure

In order to measure the sterilization rate of S. mutans, the caries-inducing bacteria, each strain was cultured in brain heart infusion (BHI) medium. When the strain reached 1.0 at wavelength 600nm through the logarithmic growth phase, the culture medium was centrifuged to obtain cells. The obtained cells were washed three times with 10 mM phosphate buffer (pH 7.0), the final concentration in the deep seawater of Preparation Example 1 was 1.0 × 10 ⁷ CFU / mL, and then plated flatly in 100 μL of the BHI medium. After 2, 4, 6, 8, and 10 hours at 37 ℃, the number of colonies (clusters) formed in the medium was counted to examine the survival rate, the results are shown in Table 7.

Experimental Example  4. Oral Bacteria during Mouthwash Treatment Survival rate  Measure

Instead of using the deep sea water, except for using the oral cleanser prepared in Examples 1 to 3 and Comparative Example 1, in the same manner as in Experiment 3, the oral bacterial survival rate is shown in Table 8 below .

Example  4. Deodorant Manufacturing

Raw water of Preparation Example 1 and TiO ₂ and hydrogen peroxide (H ₂ O ₂ ) were mixed in the component ratio as shown in Table 9 to prepare a deodorant.

function Ingredient Example 4-1 Content (g) Example 4-2 Content (g) Example 4-3 Content (g) Main solution enemy 100 99.45 99.95 Deodorant ingredients TiO ₂ - 0.5 - Deodorant ingredients H ₂ O ₂ - 0.05 0.05

Example  5. Deodorant Manufacturing

As a deep sea water, instead of using raw water, except for using the permeated water of Preparation Example 1, by mixing each component in the same proportion as in Example 4 (Example 5-1 to Example 5-3 ) Was prepared.

Example 6. Deodorant Preparation

As a deep sea water, instead of using raw water, except that deep water of hardness 250 of Preparation Example 2 was used, each component was mixed in the same ratio as in Example 4 to deodorant (Examples 6-1 to 6). -3) was prepared.

Comparative Example 2. Preparation of Deodorant

As shown in Table 10 below, a deodorant of conventional composition (content per 100 mL of deodorant product) was prepared.

Experimental Example  5. Deodorant methyl Mercaptain  Removal rate measurement

Instead of using the deep sea water, except for using the deodorant prepared in Examples 4 to 6 and Comparative Example 2, in the same manner as in Experimental Example 1-2, methyl mercaptain removal rate was measured in Table 11 below Indicated.

Experimental Example  6. Antibacterial test of deep sea water

The deep sea water (raw water, concentrated water, permeated water, and a hardness of 200, 500 and 1000 of the deep sea water), and then the purified water, each with sterilized at 121 ℃ for 15 minutes, and Escherichia coli ^{(E. coli) 1.0 x 10 4} ~ 3.0 x 10 4 CFU / mL and incubated for 24 hours. The number of bacteria formed in each medium was counted (unit of the number of bacteria: CFU / mL) to examine the removal rate, and the results are shown in Table 12 below.

Experimental Example  7. Antibacterial test of deodorant

Instead of using the deep sea water, except for using the deodorant prepared in Examples 4 to 6 and Comparative Example 2, in the same manner as in Experimental Example 6, the bacterial removal rate (antibacterial rate) was measured by the following Table 13 ( Unit number of bacteria: CFU / mL).

Example  7. Extraction, Purification and Oral Cleaning of Deep Sea Water

Raw water (ocean deep water) collected at a depth of 500 m in the East Sea of Korea is precisely filtered with a micro filter (material: Polytetrafluoroethylene (PTFE), pore size: about 0.5 ㎛, Saehan Co., Ltd.) to remove impurities, and then reverse osmosis system (Dow Chemical) Company, FILMTEC, SW30-4021) was used to separate the primary concentrated water and primary filtered water. The primary filtrate was filtered again using a reverse osmosis system to obtain secondary concentrated water and secondary filtered water. In addition, three kinds of mouthwashes were prepared by the composition (unit: wt%) shown in Table 14 below. At this time, the raw water and the primary concentrated water were used to dilute the final salinity to 1% by weight.

Materials Mouthwash A Mouthwash B Mouthwash C Primary concentrated water 16.06 15.37 15.37 Depth of Hardness 300 77.53 74.22 74.19 Sodium fluoride 0.02 0.02 0.05 Glycerin 3.00 5.00 5.00 Saccharin 0.01 0.01 0.10 Citric acid 0.05 0.05 0.01 Sodium laurylsulfate 0.03 0.03 - Sodium citrate 0.04 0.04 0.10 Ethanol 3.00 5.00 5.00 Xylitol 0.20 0.20 0.10 Flavor 0.03 0.03 0.05 Pigmentation (blue 1) 0.03 0.03 0.03 Sum 100 100 100

The mineral content (unit: ppm) of the filtrate, raw water, concentrated water and oral cleanser thus obtained is shown in Table 15 below.

Mineral content Primary Filtrate 2nd filtered water enemy Primary concentrated water Mouthwash A Mouthwash B Mouthwash C Ca 4.2 0 125.4 131.8 108.7 104.0 104.0 K 3.2 0 106.7 110.4 97.8 93.6 93.6 Mg 4.5 0 354.0 356.8 300.6 287.7 287.7 Na 78.0 0.8 3021.1 3149.3 2271.4 2173.8 2173.8 Cl 152.5 1.8 5569.7 5328.8 3955.7 3785.8 3785.7 SO4 3.6 0.1 823.1 922.9 706.2 675.9 675.8 Sum 246.0 2.7 10000.0 10000.0 7440.5 7120.9 7120.7

Experimental Example  8. Efficacy test of mouthwash

In the case of using a mouthwash prepared with deep sea water, the bacterial bacterial count, the gingival index and the bacterial bacterial membrane pH were confirmed by clinical experiments. Clinical trials were conducted from October 2006 to May 2007 in the dental clinic of Kangdong Sacred Heart Hospital. During the trial, the use of antibiotics and oral care that may affect the experiment were banned. The test subjects used seven kinds of mouthwashes for four weeks, but three times a day, morning, lunch, and dinner, three times a day with mouthwashes in the mouth and spit gargled.

A. Dental plaque  Degree of formation and Gingival index  Measure

Before oral cleaning agent, subjects were evaluated for dental plaque index score and gingival index score, and after 4 weeks of oral cleaning agent, it was evaluated again. Seven subjects were assigned to each sample. The plaque membrane was examined using plaque check colorant (Plaque check, Hu-Friedy NFG Co.), and the target teeth were the maxillary right second premolar, maxillary right canine, maxillary left first molar, mandibular left side incisor and mandibular left first. 1 premolar, mandibular right first molar. The degree of adhesion of the dental plaque was evaluated according to the method of Quigley and Hein, and the mean and standard deviation are shown in Table 16. The gingival index was evaluated according to the method of Loe and silness, and the mean and standard deviation are shown in Table 17. The paired t-test was performed on the difference between the measured value at the first examination and the measured value after 4 weeks. (Reference literature: Functional food functional test guide, tooth-related functional test: p773-796, Publisher: Korea) Korean Institute of Health Procedures, May 2004).

Before use after use % Removal Distilled water 1.15 ± 0.47 1.03 ± 0.36 10.4 Primary Filtrate 1.08 ± 0.51 0.78 ± 0.67 27.8 2nd filtered water 1.31 ± 0.44 0.95 ± 0.56 27.5 enemy 1.25 ± 0.49 0.71 ± 0.23 43.2 Primary concentrated water 1.65 ± 0.63 0.85 ± 0.43 48.5 Mouthwash A 1.52 ± 0.55 0.46 ± 0.23 * 69.7 Mouthwash B 1.23 ± 0.52 0.53 ± 0.34 * 56.9 Mouthwash C 1.21 ± 0.45 0.42 ± 0.36 * 65.3

In Table 16, the numerical values showing the degree of plaque forming on the surface of the plaque are: 0: no plaque on the gingiva, 1: when the plaque is attached on the gingival edge, 2: when the plaque is attached on the gingivitis, 3: When the bacterial plaque is attached up to 1/3 of the cervical side, 4: The dental plaque is attached up to 2/3 of the cervical side, and the * mark is statistically valid at the paired t-test at the level 0.05.

Before use after use % Removal Distilled water 0.54 ± 0.23 0.52 ± 0.21 3.7 Primary Filtrate 0.51 ± 0.21 0.49 ± 0.18 3.9 2nd filtered water 0.53 ± 0.24 0.50 ± 0.22 5.7 enemy 0.59 ± 0.19 0.49 ± 0.23 16.9 Primary concentrated water 0.59 ± 0.21 0.45 ± 0.24 23.7 Mouthwash A 0.56 ± 0.19 0.42 ± 0.21 25.0 Mouthwash B 0.53 ± 0.22 0.43 ± 0.22 18.9 Mouthwash C 0.52 ± 0.23 0.41 ± 0.21 21.2

In Table 17, the numerical values representing the gingival index are 0: no inflammatory response, 1: mild gingivitis, slight color change, 2: moderate gingivitis, surface gloss and redness, edema, swelling or bleeding due to pressure , 3: severe gingivitis, prominent redness and swelling, spontaneous bleeding and ulceration.

From Tables 16 and 17, when control distilled water and deep sea water were used as the oral cleanser, the dental plaque and gingivitis removal rate was low in distilled water, primary filtered water and secondary filtered water with low mineral content, and raw water with high mineral content. And it can be seen that the effect is high in the secondary concentrated water. In addition, in the case of oral cleaning agents, the A product having a high mineral content was higher than the B and C products, the effect of reducing the plaque formation and the gingival index. The oral cleanser C product was more effective than the B product because the mineral content of the two products is the same, but it is believed that the C product has a high NaF content.

B. Measurement of Plasma Bacterial pH Change

After brushing the surface of the test subjects, brushing is prohibited for 3 days on the site where the bacterial bacterial membrane is to be collected, the test and control samples are placed in the oral cavity for 3 minutes, and then the accumulated dental bacterium is squeezed, 1 minute from the tongue, The sample was taken after 3, 5, 10 and 20 minutes. After diluting the extracted dental plaque with 0.2 ml of distilled water, the pH change was measured with a pH meter, and the results are shown in Table 18.

1 minute later 3 minutes later 5 minutes later 10 minutes later 20 minutes later Distilled water 5.12 5.34 5.38 5.67 5.66 Primary Filtrate 5.15 5.23 5.26 5.67 5.65 2nd filtered water 5.21 5.39 5.34 5.62 5.65 enemy 5.28 5.32 5.24 5.57 5.73 Primary concentrated water 5.11 5.13 5.43 5.42 5.72 Mouthwash A 5.20 5.36 5.30 5.66 5.72 Mouthwash B 5.21 5.2 5.34 5.39 5.72 Mouthwash C 5.11 5.26 5.35 5.71 5.73

As shown in Table 18, the pH value does not vary significantly depending on the sample, and the test solution can be gargled once, and within 1 minute after gargle, the pH of the dental plaque can be increased to 5.5, the demineralization threshold pH of the enamel. There was no. After 20 minutes of goggle, the sample with increased pH of the dental plaque is higher than pH 5.7 (the functional test guide for health functional food, Korea Institute of Health Research), which is the statutory standard of the standard test method for dental health functionalities. Three types of raw water, secondary concentrated water and oral cleanser prototypes were used.

As described above, as a result of clinical experiments on the formation of gingival bacterium, the gingival index and the pH of the gingival bacterium, the higher the mineral content of the deep ocean water and the oral cleaning agent containing the same, the more the gingival bacterial membrane formation was inhibited, the gingival index decreased, It can be seen that the pH of the bacterial membrane is increased.

Claims (5)

A mouthwash containing deep sea water as an active ingredient. The mouthwash according to claim 1, wherein the deep sea water has a hardness of 100 to 13,000. The oral cleanser according to claim 2, wherein the deep sea water having a hardness of 100 to 13,000 is mixed with one or two or more deep sea water selected from the group consisting of raw water, concentrated water, permeated water and purified water. Deodorant containing deep sea water as an active ingredient. According to claim 4, 0.001 to 0.05 parts by weight of hydrogen peroxide (H ₂ O ₂ ) relative to 100 parts by weight of the deep sea water, 0.01 to 0.5 parts by weight of titanium oxide (TiO ₂ ) and mixtures thereof based on 100 parts by weight of deep sea water Deodorant further comprising a deodorant compound selected from the group consisting of.