CN115025015A

CN115025015A - Antibacterial mouth wash and preparation method thereof

Info

Publication number: CN115025015A
Application number: CN202210587840.7A
Authority: CN
Inventors: 吴岗; 周海林; 郭秀玲; 姜媛媛
Original assignee: Shenzhen Anduofu Disinfection High Tech Co ltd
Current assignee: Shenzhen Anduofu Disinfection High Tech Co ltd
Priority date: 2022-05-27
Filing date: 2022-05-27
Publication date: 2022-09-09
Anticipated expiration: 2042-05-27
Also published as: CN115025015B

Abstract

The application discloses an antibacterial mouth wash and a preparation method thereof, and belongs to the field of oral care. An antibacterial mouth wash is composed of the following raw materials in percentage by weight: 0.25-1% of polyvinylpyrrolidone iodine, 0.5-2% of menthol, 0.01-0.1% of xylitol, 0.05-0.2% of surfactant, 1-5% of humectant, 0.1-1% of preservative, 0.05-0.2% of pH regulator and the balance of water. A preparation method of antibacterial mouthwash comprises the following steps: adding polyvinylpyrrolidone iodine, menthol, xylitol and humectant into water, stirring and mixing, then continuously adding surfactant and pH regulator, stirring and mixing, finally adding preservative, stirring and mixing to obtain the antibacterial mouthwash. The application has the advantages of keeping the comfort when the mouthwash is used and obtaining good sterilizing effect.

Description

Antibacterial mouth wash and preparation method thereof

Technical Field

The application relates to the field of oral care, in particular to antibacterial mouthwash and a preparation method thereof.

Background

Mouthwash is a common oral care product except toothpaste and dentifrice, and is mainly used for cleaning the oral cavity, inhibiting the propagation of bacteria in the oral cavity, covering up peculiar smell in the oral cavity and leaving a comfortable and refreshing feeling in the oral cavity. Along with the continuous attention of people to personal image and social etiquette, the market demand of mouthwash is also increasing day by day, and function and the quality requirement to the mouthwash also constantly improve.

The main components of the mouthwash comprise functional auxiliaries, essence, a humectant, a surfactant, a preservative, a solvent and the like, wherein the functional auxiliaries can be an antibacterial agent, an anti-caries agent, a gingivitis prevention compound, an anti-inflammatory agent and the like so as to realize the functions of sterilization, inflammation diminishing and infection resistance of the mouthwash, the surfactant can realize the function of cleaning the oral cavity of the mouthwash, and the essence can realize the function of covering bad smells such as halitosis and the like of the mouthwash, so that the effect of the mouthwash is relatively comprehensive.

At present, the common mouthwash in the market generally can add cetylpyridinium chloride as bacteriostatic agent, and cetylpyridinium chloride has the effect of antibacterial disinfection, improves the bactericidal effect of mouthwash, and the condition of the mucous membrane that keeps the oral cavity moist can be destroyed to the addition of cetylpyridinium chloride to arouse the sense of stinging, make the application and popularization of mouthwash receive the restriction.

Disclosure of Invention

In order to keep the mouth wash comfortable and obtain good sterilizing effect when in use, the application provides an antibacterial mouth wash and a preparation method thereof.

In a first aspect, the antibacterial mouth wash provided by the application adopts the following technical scheme:

an antibacterial mouth wash is composed of the following raw materials in percentage by weight:

0.25 to 1 percent of polyvinylpyrrolidone iodine;

0.5 to 2 percent of menthol;

0.01 to 0.1 percent of xylitol;

0.05 to 0.2 percent of surfactant;

1 to 5 percent of humectant;

0.1 to 1 percent of preservative;

0.05 to 0.2 percent of pH regulator;

0 to 0.6 percent of bacteriostatic synergist;

0 to 0.5 percent of other additives;

the balance of water.

By adopting the technical scheme, the application uses the polyvinylpyrrolidone iodine as the bacteriostatic agent, the polyvinylpyrrolidone iodine belongs to broad-spectrum bactericidal agents, pathogenic microorganisms such as bacteria, viruses and fungi are effectively killed, the low toxicity is achieved, and no stimulation is caused to oral mucosa, so that the mouth wash is kept comfortable when in use and obtains a good bactericidal effect.

The application adds menthol and xylitol in the mouthwash, and the menthol can clean the oral cavity, reduces the gum sensitivity, and the decayed tooth can be prevented to xylitol to antibacterial effect is outstanding, can restrain the bacterium and adsorb at the tooth surface and form dental plaque, and menthol and xylitol and polyvinylpyrrolidone iodine act together, can improve the antibacterial performance that disinfects of mouthwash greatly.

Optionally, the antibacterial synergist comprises a snow tea extract, hydroxypropyl chitosan and ethanol, wherein the weight ratio of the polyvinylpyrrolidone iodine to the snow tea extract to the hydroxypropyl chitosan to the ethanol is 1 (0.45-0.63) to (0.20-0.32) to (1.2-1.5).

Through adopting above-mentioned technical scheme, contain snow tea extract in, snow tea extract has good antibacterial activity's material such as snowtea extract, depside, can effectively reduce the germ in the oral cavity to snow tea extract cooperates with hydroxypropyl chitosan, can further clear away the peculiar smell in the oral cavity, keeps the breath fresh for a long time, and snow tea extract is less to the stimulation of oral mucosa, uses the comfort to improve moreover.

Optionally, the antibacterial synergist comprises ethyl palmitate and ethanol, and the weight ratio of the xylitol to the ethyl palmitate to the ethanol is 1 (1.2-1.6) to 2-3.

By adopting the technical scheme, the ethyl palmitate can help the xylitol to disperse and stabilize, thereby improving the inhibition effect on the dental plaque dispersion and keeping the teeth clean.

Optionally, the surfactant is sodium methyl cocoyl taurate.

By adopting the technical scheme, the sodium methyl cocoyl taurate belongs to amino acid type surfactants, has a good cleaning function, is beneficial to removing food residues between teeth, and has small irritation to oral mucosa and good use experience.

Optionally, the preservative is methylparaben.

By adopting the technical scheme, the erosion condition of the mouthwash can be reduced by adding the methyl hydroxybenzoate, and a long-acting antibacterial effect is achieved.

Optionally, the pH adjusting agent is citric acid.

By adopting the technical scheme, the citric acid can adjust the pH balance in the oral cavity and is beneficial to removing the fishy smell in the oral cavity.

Optionally, the humectant is sorbitol.

Optionally, the other additives include flavorants and sweeteners.

Through adopting above-mentioned technical scheme, essence and sweetener can improve the taste of mouthwash, comfort when further improving the use.

Optionally, the sweetener is stevioside.

Optionally, the essence is lemon essence.

In a second aspect, the preparation method of the antibacterial mouthwash provided by the application adopts the following technical scheme:

a preparation method of antibacterial mouth wash comprises the following steps:

adding polyvinylpyrrolidone iodine, menthol, xylitol and humectant into water, stirring and mixing, then continuously adding surfactant and pH regulator, stirring and mixing, finally adding preservative, stirring and mixing to obtain the antibacterial mouthwash.

By adopting the technical scheme, the adding sequence of the raw materials is controlled, so that the raw materials are fully dispersed in water, and the stable mouthwash with good function effect is obtained.

Optionally, before adding the povidone iodine, the menthol, the xylitol and the humectant into the water, adding the antibacterial synergist into the water, and stirring and mixing; the bacteriostatic synergist comprises a snow tea extract, hydroxypropyl chitosan and ethanol, wherein the weight ratio of polyvinylpyrrolidone iodine to the snow tea extract to the hydroxypropyl chitosan is 1 (0.45-0.63) to (0.2-0.32) to (1.2-1.5).

By adopting the technical scheme, before polyvinylpyrrolidone iodine, menthol, xylitol and the humectant are added into water, the snow tea extract and hydroxypropyl chitosan are fully mixed and dispersed, so that the synergistic effect of the snow tea extract and the hydroxypropyl chitosan is favorably exerted, and the oral odor is continuously reduced for a long time.

Optionally, before adding the povidone iodine, the menthol, the xylitol and the humectant into the water, adding the antibacterial synergist into the water, and stirring and mixing; the antibacterial synergist comprises ethyl palmitate and ethanol, wherein the weight ratio of the xylitol to the ethyl palmitate to the ethanol is 1 (1.2-1.6) to 2-3.

Optionally, the snow tea is dried, cut up, added into ethanol for ultrasonic extraction, filtered, and concentrated to obtain the snow tea extract.

Optionally, the ultrasonic leaching time is 0.5-1 h, the ultrasonic frequency is 50-70 KHz, preferably, the ultrasonic leaching time is 1h, and the ultrasonic frequency is 70 KHz.

By adopting the technical scheme, most of the snowfield tea extract is insoluble in water, so that the active bacteriostatic substances in the snowfield tea can be extracted by extracting the snowfield tea with ethanol.

In summary, the present application has the following beneficial effects:

1. the application uses the polyvinylpyrrolidone iodine as the bacteriostatic agent, and the polyvinylpyrrolidone iodine belongs to broad-spectrum bactericidal agents, effectively kills pathogenic microorganisms such as bacteria, viruses and fungi, has low toxicity and no stimulation to oral mucosa, thereby keeping the comfort when the mouthwash is used and obtaining good bactericidal effect.

According to the application, the menthol and the xylitol are added into the mouthwash, the menthol can clean the oral cavity and reduce the gum sensitivity, the xylitol can prevent decayed teeth, the antibacterial effect is remarkable, bacteria can be inhibited from being adsorbed on the surfaces of teeth to form dental plaque, and the menthol, the xylitol and the polyvinylpyrrolidone iodine act together, so that the sterilization and antibacterial performance of the mouthwash can be greatly improved;

2. the snowflake tea extract and hydroxypropyl chitosan are added, so that the bacteriostatic ability is improved, and the peculiar smell of the oral cavity is further removed.

Detailed Description

The present application will be described in further detail with reference to examples and comparative examples.

Preparation example

The preparation method of the snow tea extract comprises the following steps:

drying the snow tea in a 65 ℃ oven for 1.5h, taking out and cutting the snow tea, adding the broken snow tea blocks into ethanol, ultrasonically extracting for 1h, wherein the ultrasonic frequency is 70kHz, filtering, collecting filtrate, rotatably steaming and concentrating the filtrate, and vacuumizing to remove the ethanol to obtain the powdered snow tea extract.

Examples

Example 1

An antibacterial mouth wash is prepared from the following raw materials:

25g of polyvinylpyrrolidone iodine, 50g of menthol, 1g of xylitol, 5g of sodium methyl cocoyl taurate, 10g of methylparaben, 100g of sorbitol, 5g of citric acid and 9804g of water.

A preparation method of antibacterial mouthwash comprises the following steps:

adding polyvinylpyrrolidone iodine, menthol, xylitol and sorbitol into water, stirring and mixing for 5min, then continuously adding sodium methyl cocoyl taurate and citric acid, stirring and mixing for 5min, finally adding methyl hydroxybenzoate, stirring and mixing for 2min to obtain the antibacterial mouth wash.

Example 2

An antibacterial mouth wash is prepared from the following raw materials:

100g of polyvinylpyrrolidone iodine, 200g of menthol, 10g of xylitol, 20g of sodium methyl cocoyl taurate, 100g of methylparaben, 500g of sorbitol, 20g of citric acid and 9050g of water.

A preparation method of antibacterial mouthwash comprises the following steps:

Example 3

An antibacterial mouth wash is prepared from the following raw materials:

60g of polyvinylpyrrolidone iodine, 130g of menthol, 6g of xylitol, 14g of sodium methyl cocoyl taurate, 40g of methylparaben, 335g of sorbitol, 16g of citric acid and 9399g of water.

A preparation method of antibacterial mouthwash comprises the following steps:

Example 4

The difference between this example and example 3 is that the antibacterial mouth wash has different raw materials and preparation method.

The antibacterial mouth wash also comprises 57g of snow tea extract and 90g of ethanol, wherein the weight of water is 9252 g.

The antibacterial collutory is prepared by adding polyvinylpyrrolidone iodine, menthol, xylitol and sorbitol into water, mixing herba Centellae extract and ethanol, adding into water, and stirring for 10 min.

Example 5

The difference between this example and example 3 is that the antibacterial mouthwash is different in raw materials and preparation method.

The antibacterial mouth wash also comprises 57g of hydroxypropyl chitosan and 90g of ethanol, wherein the weight of water is 9252 g.

In the preparation method of the antibacterial mouth wash, before polyvinylpyrrolidone iodine, menthol, xylitol and sorbitol are added into water, hydroxypropyl chitosan and ethanol are mixed and then added into water, and the mixture is stirred and mixed for 10 min.

Example 6

The raw materials of the antibacterial mouth wash also contain an antibacterial synergist, and the antibacterial synergist is prepared by mixing a snow tea extract, hydroxypropyl chitosan and ethanol, wherein 37.8g of the snow tea extract, 19.2g of the hydroxypropyl chitosan and 90g of the ethanol are added, and the weight of water is 9252 g.

The antibacterial collutory is prepared by adding antibacterial synergist into water before adding polyvinylpyrrolidone iodine, menthol, xylitol and sorbitol into water, and stirring and mixing for 10 min.

Example 7

The difference between this example and example 6 is that the antibacterial mouth wash has different raw material ratios.

In the bacteriostasis synergist of the embodiment, 27g of the snow tea extract, 12g of hydroxypropyl chitosan and 72g of ethanol are adopted, and the weight of water is 9288 g.

Example 8

The antibacterial mouthwash also comprises an antibacterial synergist which is formed by mixing ethyl palmitate and ethanol, wherein the weight of the ethyl palmitate is 7.2g, the weight of the ethanol is 12g, and the weight of the water is 9379.8 g.

Example 9

The difference between this example and example 8 is that the antibacterial mouth wash has different raw material ratios.

In the bacteriostatic synergist of this example, 9.6g of ethyl palmitate and 18g of ethanol were added, and the weight of water was 9371.4 g.

Example 10

The difference between this example and example 6 is that the antibacterial mouth wash has different raw material compositions and proportions.

The bacteriostat synergist of the embodiment is composed of snow tea extract, hydroxypropyl chitosan, ethyl palmitate and ethanol, wherein 37.8g of snow tea extract, 19.2g of hydroxypropyl chitosan, 9.6g of ethyl palmitate and 108g of ethanol are added, and the weight of water is changed to 9224.4 g.

Example 11

The antibacterial mouth wash also comprises other additives, wherein the other additives comprise edible essence and sweetening agent, the edible essence is lemon essence, the lemon essence is 5g, the sweetening agent is stevioside, the stevioside is 5g, and the weight of the water is 9389 g.

The antibacterial collutory is prepared by adding antiseptic into water, adding other additives into water, and stirring and mixing for 10 min.

Example 12

The difference between this example and example 11 is that the antibacterial mouth wash has different raw material compositions and proportions.

In the other additives of the embodiment, the weight of the lemon essence is 30g, the weight of the stevioside is 20g, and the weight of the water is 9249 g.

Comparative example

Comparative example 1

The difference between the comparative example and the example 3 is that the raw material composition and the mixture ratio of the antibacterial mouth wash are different.

The raw materials of the antibacterial mouth wash are not added with polyvinylpyrrolidone iodine, and the weight of the water is 9459 g.

Comparative example 2

The antibacterial mouth wash is prepared by adding no xylitol into the raw materials, wherein the weight of menthol is changed to 20g, and the weight of water is changed to 9515 g.

Comparative example 3

The difference between the comparative example and the example 6 is that the raw material composition and the mixture ratio of the antibacterial mouth wash are different.

The raw materials of the antibacterial mouth wash are not added with polyvinylpyrrolidone iodine, and the weight of the water is 9402 g.

Effect detection test

The mouthwashes prepared in the various examples and comparative examples of the present application were tested.

Antibacterial tests were carried out according to WS/T650-plus 2019 antibacterial and bacteriostatic effect evaluation method '5.2.1 suspension quantitative bactericidal test', the antibacterial rate was tested, the test strains were Escherichia coli and Candida albicans, the action concentration of the mouthwash sample was the stock solution, the action time was 1min, and the test results are shown in Table 1.

The long-acting antibacterial test is carried out according to WS/T650-plus 2019 (evaluation method for antibacterial and bacteriostatic effects) '5.2.7 continuous antibacterial test', the long-acting antibacterial rate is tested, the test strains are escherichia coli and candida albicans, the mouth wash sample is stored at room temperature for 12h and then tested, the action concentration of the mouth wash sample is the stock solution, the action time is 1min, and the test results are shown in Table 1.

Selecting 90 volunteers without oral diseases and between the ages of 25-35, randomly and averagely dividing the volunteers into 15 groups, respectively testing 15 kinds of mouthwash prepared in the embodiment and the comparative example by each group of volunteers, firstly, sampling the odor of a sampling cup, then smelling the odor of the sampling cup, evaluating and scoring the odor degree in the oral cavity, wherein the score ranges from 0 to 10, the higher the score is, the heavier the odor degree is, then, gargling for 1min by using the mouthwash, spitting out the mouthwash, not eating within 8h after gargling, and after 8h, again, the volunteers breath the sampling cup, evaluating and scoring the odor degree in the oral cavity, calculating the difference between the two scoring before and after the two times as an odor removal value, taking the average value of the odor removal values of each group, and recording the result is shown in table 2.

15 volunteers 25-35 years old without oral diseases are selected, on the 1 st day of the experiment, the tooth with the number of 16 is divided into 0 in the plaque index (PLI) test by means of tooth brushing and the like, then normally work and rest for 2 days without brushing teeth, and rinse with mouth wash for 1min each morning and night, each volunteer corresponds to 15 kinds of mouth wash prepared by the examples and comparative examples of the present application, and the plaque index test is performed again on the teeth of each volunteer with the number of 16 by day 3, the scoring results are shown in table 2, the scoring criteria were "0 ═ plaque free in the marginal area, 1 ═ plaque thin on the tooth surface but not visible by visual inspection, and when the tooth surface was scraped with a probe tip, 2 ═ moderate plaque visible at the marginal or adjacent surface, 3 ═ in the sulcus or at the marginal and adjacent surfaces, and 3 ═ a lot of soft plaque, as shown in table 2.

15 volunteers without oral diseases and aged 25-35 years are selected alternatively, each volunteer respectively corresponds to 15 kinds of mouthwash prepared by the embodiment and the comparative example, the mouth is rinsed for 1min, the tingling sensation of the mouthwash is evaluated, the tingling sensation or no tingling sensation is evaluated, and the test results are shown in table 2.

TABLE 1

TABLE 2

	Oral odor test	Index of bacterial plaque	Evaluation of tingling sensation
				Example 1	4.2	1	No stinging sensation
Example 2	4.0	1	No stinging sensation
				Example 3	4.3	1	No stinging sensation
Example 4	3.8	1	No stinging sensation
				Example 5	4.5	1	No stinging sensation
Example 6	6.5	1	No stinging sensation
				Example 7	6.3	1	No stinging sensation
Example 8	5.0	0	No stinging sensation
				Example 9	5.3	0	No stinging sensation
Example 10	6.7	0	No stinging sensation
				Example 11	4.7	1	No stinging sensation
Example 12	4.2	1	No stinging sensation
				Comparative example 1	3.3	1	No stinging sensation
Comparative example 2	2.0	2	No stinging sensation
				Comparative example 3	4.7	1	No stinging sensation

As can be seen from tables 1 and 2, in example 3, in comparison with comparative example 1, when povidone-iodine was added, the mouthwash had good effects in antibacterial ability, oral malodor removing ability, and plaque inhibiting ability, and had no stinging sensation.

Compared with the comparative example 2, in the example 3, on the basis of adding the povidone-iodine, the menthol and the xylitol are added in the ranges of (0.5% -2%) and (0.01% -0.1%), so that more excellent antibacterial ability and oral odor removing ability are obtained.

Compared with the embodiment 3, the snow tea extract is added in the embodiment 4, so that the mouth wash is improved in long-acting antibacterial ability; compared with the embodiment 3, the hydroxypropyl chitosan is added in the embodiment 5, so that the mouthwash has no obvious effect improvement; compared with the embodiment 3, the snow tea extract and hydroxypropyl chitosan are added into the mouth wash in the embodiments 6 to 7, so that the long-acting antibacterial capacity of the mouth wash is improved greatly, the mouth odor removing capacity is improved obviously, and the effect is more prominent.

Compared with the comparative example 3, the mouthwash contains the polyvinylpyrrolidone iodine, the snow tea extract and the hydroxypropyl chitosan, and the mouthwash has good effects on the antibacterial capacity and the oral odor removing capacity, so that the polyvinylpyrrolidone iodine, the snow tea extract and the hydroxypropyl chitosan can supplement each other to exert the effects.

Compared with example 3, the mouthwash added in examples 8-9 has obviously improved plaque inhibiting capacity due to the addition of ethyl palmitate.

Compared with the example 3, the example 10 simultaneously adds the ethyl palmitate, the snow tea extract and the hydroxypropyl chitosan, and the comprehensive effect is optimal.

The present embodiment is only for explaining the present application, and it is not limited to the present application, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present application.

Claims

1. An antibacterial mouthwash is characterized in that: the composite material consists of the following raw materials in percentage by weight:

0.25% -1% of polyvinylpyrrolidone iodine;

0.5% -2% of menthol;

0.01% -0.1% of xylitol;

0.05% -0.2% of a surfactant;

1% -5% of a humectant;

0.1% -1% of preservative;

0.05% -0.2% of pH regulator;

0% -0.6% of antibacterial synergist;

0-0.5% of other additives;

the balance of water.

2. An antibacterial mouthwash according to claim 1, wherein: the antibacterial synergist comprises a snow tea extract, hydroxypropyl chitosan and ethanol, wherein the weight ratio of polyvinylpyrrolidone iodine to the snow tea extract to the hydroxypropyl chitosan to the ethanol is 1 (0.45-0.63) to 0.20-0.32 to 1.2-1.5.

3. An antibacterial mouthwash according to claim 1 or 2, wherein: the antibacterial synergist comprises ethyl palmitate and ethanol, wherein the weight ratio of the xylitol to the ethyl palmitate to the ethanol is 1 (1.2-1.6) to 2-3.

4. An antibacterial mouthwash according to claim 1, wherein: the surfactant is sodium methyl cocoyl taurate.

5. An antibacterial mouthwash according to claim 1, wherein: the preservative is methyl hydroxybenzoate.

6. An antibacterial mouthwash according to claim 1, wherein: the pH regulator is citric acid.

7. An antibacterial mouthwash according to claim 1, wherein: the other additives include flavorants and sweeteners.

8. A preparation method of antibacterial mouth wash is characterized by comprising the following steps: the process for the preparation of the antibacterial mouthwash according to any one of claims 1 to 7, comprising the following steps:

9. The method for preparing antibacterial mouth wash according to claim 8, wherein the antibacterial mouth wash comprises the following steps: adding the antibacterial synergist into water and stirring and mixing before adding the polyvinylpyrrolidone iodine, the menthol, the xylitol and the humectant into the water; the bacteriostatic synergist comprises a snow tea extract, hydroxypropyl chitosan and ethanol, wherein the weight ratio of polyvinylpyrrolidone iodine to the snow tea extract to the hydroxypropyl chitosan is 1 (0.45-0.63) to (0.2-0.32) to (1.2-1.5).

10. The method for preparing antibacterial mouth wash according to claim 9, wherein the antibacterial mouth wash comprises the following steps: the preparation method of the snow tea extract comprises the following steps: drying herba Centellae, cutting, adding into ethanol, ultrasonic extracting, filtering, and concentrating the filtrate to obtain herba Centellae extract.