KR100769573B1 - Composition for tablet oral cavity washing agent containing enzymes - Google Patents

Abstract

The present invention relates to a tablet-type mouthwash composition comprising an enzyme, glucose oxidase, dextranase, lactoperoxidase, lactoperoxidase, gluconase, lysozyme, Provided is a tablet-type mouthwash composition comprising an enzyme selected from the group consisting of proteases. Tablet-type mouthwashes of the present invention to maintain the activity of the enzyme stable and excellent in plaque removal, bad breath removal and bad breath occurrence effect, easy to use and easily portable.

Enzyme, plaque removal, bad breath removal, tablet mouthwash

Description

Tablet-type mouthwash composition containing enzyme {COMPOSITION FOR TABLET ORAL CAVITY WASHING AGENT CONTAINING ENZYMES}

The present invention relates to a tablet-type mouthwash composition comprising an enzyme, and more particularly includes glucose oxidase, dextranase, lactoperoxidase, glucanase, lysozyme or protease, which are effective for removing plague and bad breath. It relates to a tablet-type mouthwash composition.

  Bad breath is caused by acquired systemic diseases, or protein or food waste in saliva is decomposed into amino acids by microorganisms in the oral cavity, and then amino acids are decomposed by decalcification or amino enzymes to produce substances that cause odor. do. In addition, when ingesting garlic or red pepper, bad breath is also caused by sulfides contained in these substances.

Plaque is a white or tan brown light deposit attached to the surface of a tooth and consists of many oral bacteria and interbacterial substrates. The interbacterial substrate is composed of polysaccharides produced by bacteria, glycoproteins in saliva, food residues, and proteins in effusions from gingival cells. In addition, decellularized epithelial cells are often mixed in the bacterial intercellular matrix. The plaque is formed in large quantities in incomplete parts of the mechanical cleaning, such as the cervical region, the proximal surface, and the main portion of the bovine and fissure.

The formation of plaques begins by first attaching glycoprotein components in saliva to the surface of the teeth in a film form, whereby bacteria are settled and multiplied. Bacteria that form early plaques are cocci, but as the plaque matures, the bacteria are mainly converted to filamentous fungi, and as the fungi proliferate, the plaques rapidly increase in thickness.

There are many types of bacteria in the plague , the main ones of which are S treptococcus , Neisseria , Veillonella , Fusobacterium , Actinomyces , and Noh. Arcadia (Nocardia), Escherichia repto tree (Leptotrichia), Bacillus kaekto (Cactobacillus), such as the Cobb the labor Ito Fe (Capnocytophaga).

Recently, the streptococcus mutans, a group of chains in prag, has attracted attention as an important bacterium that causes caries. Streptococcus mutans breaks down sugar in foods to produce acid, which is called glucan. This is because it produces polysaccharides. Glucan does not dissolve well in water and is very sticky. Therefore, glucan is believed to play a role in attaching and stagling plaque on the surface of teeth. Glucan also collects Streptococcus mutans and forms agglomerates, which can be a cause of plaque formation. Plaque is most common when oral cleanliness is incomplete and causes bad breath, caries, and periodontal disease.                         

Heavy enzymes of the active ingredient which suppress bad breath production and the production of plaque are included. However, in the case of enzymes, the efficacy is excellent, but the stability in liquid formulations is difficult to realize a lot of efficacy.

Therefore, an object of the present invention is to provide a tablet-type mouthwash composition which can remove plaque.

In addition, an object of the present invention is to provide a tablet-type mouthwash composition that maintains the stability of the enzyme and exhibits excellent mouthwashing action.

It is another object of the present invention to provide a tablet-type mouthwash composition which is simple to use and portable.

In order to achieve the above object, the present invention provides a tablet-type mouthwash composition, glucose oxidase, dextranase, lactoperoxidase, lactoperoxidase, gluconase, lysozyme ), Provides a tablet-type mouthwash composition comprising 0.01 to 50% by weight of one or more enzymes selected from the group consisting of protease.

Hereinafter, the present invention will be described in detail.

Tablet-type mouthwash composition of the present invention is glucose oxidase (GOD), dextranase (dextranase), lactoperoxidase (lactoperoxidase), glucanase, lysozyme and protease (protease) 0.01 wt% to 50 wt% of one or more enzymes selected from the group consisting of If the content of the enzyme is less than 0.01% by weight it is difficult to expect the effect by the oral enzymatic action, if the amount exceeds 50% by weight there is a risk of sterilization to the beneficial oral microorganisms by the use of excess enzyme. Most preferably, the enzyme is contained in an amount of 0.1 to 30% by weight.

The glucose oxidase inhibits plaque production by decomposing glucose producing plaque by using microorganisms in the oral cavity, and induces bactericidal action by generating hydrogen peroxide. Dextranase decomposes 1, 6- [α] -glucosidic linkages of dextran and decomposes dextran produced in the oral cavity to inhibit the production of plaque, and lactoperoxidase is used in oral lactose ( Lactose) disintegrates and produces hydrogen peroxide to sterilize. Glucanase breaks down the glucan produced by Streptococcus mutans and inhibits plaque from sticking to the teeth. Lysozyme and protease act on the microbial cell walls that live in the oral cavity and inhibit the growth of bad bacteria and plaque-producing bacteria.

In addition, the tablet mouthwash composition of the present invention is 1 to 55% by weight of the blowing agent, 0.1 to 20% by weight of the dehumidifying agent, 0.1 to 5% by weight of the pH adjuster, 1 to 5% by weight of the foaming agent, 0.1 to 10% by weight of the binder, the lubricant It is not necessary to use it according to the prescription, and when used, 0.05 to 3% by weight, the flavoring agent 0.1 to 15% by weight, the solvent may not be used according to the prescription, and when used, 0.1 to 15% by weight, and 0.001 to 10% by weight of the drug It may include.                     

The blowing agent is preferably at least one selected from the group consisting of sodium bicarbonate, ammonium bicarbonate, potassium bicarbonate and iron carbonate, has an excellent inhibitory effect on oral bacteria, and decays by neutralizing organic acids produced by plaque. Sodium bicarbonate, which has a prophylactic effect and a soft polishing power, is most preferred.

The dehumidifying agent is preferably sodium carbonate, and the pH adjuster is preferably citric acid or sodium citrate. The solvent used for dissolving the binder is preferably polypropylene glycol, glycerin or ethanol.

The foaming agent acts to drop foreign substances in the oral cavity by enhancing the feeling of use, helping to cleanse, and rapidly dispersing and penetrating other drug substances and reducing interfacial tension. The foaming agent is preferably an anionic surfactant, sodium lauryl sulfate, and a copolymer of a nonionic surfactant polyoxyethylene polyoxypropylene (polosomer), polyoxyethylene cured oil, and polyoxyethylene sorbbi depending on the characteristics of the formulation. Carbon fatty acid esters and the like can be used.

The binder is preferably a water-soluble polymer, more preferably carboxymethyl cellulose or polyvinylpyrrolidone, and the lubricant is magnesium stearate, stearic acid, stearyl alcohol, sucrose fatty acid ester, talc, hard silicic anhydride or sodium benzoate. In addition, flavoring agents include sodium saccharin, saccharin, and glycyritin, and lemons, oranges, pines, mints, menthols, and the like. Sodium fluoride or fluorine-containing compound is included as a medicament. In addition, the mouthwash composition of the present invention may further include a colorant. As a coloring agent, yellow triiron dioxide, red trioxide, tar type pigment | dye, etc. are mentioned.

The tablet mouthwash of the present invention may be carried out by a conventional method for preparing a tablet mouthwash, and preferably, the blowing agent, the moisture remover, and the pH adjuster are mixed, further mixed with a binder solution dissolved in a solvent, and then granular. It is prepared by drying, sizing, sizing and mixing with enzymes, lubricants, foaming agents, and medicaments to produce tablets. Tablet-type mouthwashes of the present invention in the form of tablets, can be prepared in a variety of release tablets having a circular tablet or a surface shape such as ordinary R surface, sugar R surface, Smeker plane, smear plane, two-stage R surface, 1 It is preferable that the tablets are 0.01-5 g, Most preferably, it is 1.0 + 0.1 g. The tablets can be washed orally by dissolving in 1 ml-2000 ml of drinking water and gargle for 30 seconds.

The tablet-type mouthwash composition of the present invention is composed of anhydride, high stability of the enzyme to maintain the original function of the enzyme, it was able to obtain a superior effect than the conventional liquid formulation when used. In addition, since the tablet-type mouthwash composition can be used by the user dissolved in water immediately before use, regardless of the place of use has the advantage of easy to carry.

    Hereinafter, preferred examples are provided to aid in understanding the present invention. However, the following examples are provided only to more easily understand the present invention, and the present invention is not limited to the following examples.

 EXAMPLE                     

The mouthwash tablets of Examples 1 to 5 were prepared using the ingredients and contents shown in Table 1. In the manufacturing method, first, a blowing agent, a dehumidifying agent and a pH adjuster were mixed, the binder was dissolved in a solvent, and the mixture was added and stirred. After stirring, granules were prepared to a constant size using a granulator and dried. After the granules were dried and mixed, enzyme powder, foaming agent, lubricant, and agonist were mixed and stirred with the dried granules to prepare 1.0 + 0.1 g of tablet.

Raw material classification Raw material name Comparative example Example 1 Example 2 Example 3 Example 4 Example 5 enzyme Glucose oxidase - 20.0 - - - - Dextranase - - 2.0 - 12.0 15.0 Lactoperoxidase - - - 5.0 - - Glucanase - - - 25.0 5.0 - Lysozyme - - 10.0 - 7.0 15.0 Protease - 9.0 - - 5.0 - blowing agent Sodium bicarbonate - 38.0 35.0 36.0 37.0 35 Dehumidifier Sodium carbonate - 8.0 10.0 9.0 9.0 9.0 pH regulator Citric acid 0.01 0.5 0.5 0.5 0.5 0.5 Sodium citrate 0.2 1.5 1.5 1.5 1.5 2.0 Foam Sodium lauryl sulfate 0.4 3.0 3.0 3.0 3.0 3.0 Binder Carboxylmethyl Cellulose - 3.0 1.0 - 2.0 2.5 Polyvinylpyrrolidone - - 2.0 3.0 1.0 - Lubricant Sodium benzoate - 1.0 1.0 1.0 1.0 1.0 Flavor Saccharin Sodium 0.01 1.0 1.0 1.0 1.0 1.0 incense 0.15 5.0 5.0 5.0 5.0 6.0 menstruum Polypropylene glycol - 5.0 5.0 - - - glycerin 12.0 - - 6.0 6.0 6.0 ethanol 8.0 3.0 3.0 2.0 2.0 2.0 Purified water 79.21 - - - - - Sodium fluoride 0.02 2.0 2.0 2.0 2.0 2.0 Medicinal agents Sum 100.0 100.0 100.0 100.0 100.0 100.0

[Comparative Example]

Tablets were prepared by the Comparative Example composition of Table 1.                     

Experimental Example

(1) Microbial Sterilization Evaluation

The bactericidal effect and the microbial growth inhibitory effect on the bad breath causing bacteria caused by the peroxide generated by the tablets of Examples 1 to 5 were confirmed.

Streptococcus mutans was inoculated in 10 ml of THB (Todd Hewitt Broth) complex 3% solution and incubated for 12 hours. 5 ml of the culture solution was inoculated into 500 ml of THB complex medium and incubated for another 12 hours. One tablet of Example 1 to Example 5 or Comparative Example was administered to 200 ml of the secondary culture solution, respectively, for 1 minute, 5 minutes, After 15 minutes and 30 minutes, the culture solution was streaked on an agar plate with a platinum rod and cultured overnight, and the growth of microorganisms was visually confirmed.

As a result, the tablets of Examples 1 to 5 confirmed the growth of the microorganisms in the agar plate after 1 minute, 5 minutes, 15 minutes, and 30 minutes after administration to the culture medium, but it was confirmed that there was no growth of the microorganisms. . On the other hand, in the case of the tablets of the comparative example, it was confirmed that the microorganisms were grown, and the tablets of Examples 1 to 5 were found to have excellent microbial sterilization effect.

(2) plaque generation inhibitory effect

The incidence of plaques was assessed by the Plaque Index, Turesky Quigley and Hein method.

Thirty-six adult women aged between 21 and 35 years of age with relatively even dentition and no dental caries were selected as subjects and divided into six groups of six individuals with even grades. To create the same condition, all subjects in Group 6 were provided with standard toothpaste and toothbrushes for one week. At this time, toothpaste and toothbrushes were used three times a day and the use of oral hygiene products except toothpaste and toothbrushes were discontinued. The standard toothpaste and toothbrush were used three times a day, and the tablets of Examples 1 to 5 were allowed to goggle five times daily for 30 seconds or more for four weeks. Four weeks later, the plaques attached to the teeth of all subjects were stained with a dental disclosing solution, and the plaques of the buccal and lingual surfaces of the individual teeth were evaluated based on the following criteria, and the plaque indexes are shown in Table 2 below. It was.

Rating criteria

0 points: If there is no biofilm

1 point: If there is a bacterial film on the gingival edge

2 points | pieces: When there is a bacterial film | membrane when it is lined clearly along the gingival margin

3 points | pieces: When there is a bacterial film on the tooth surface below 1/3 of the cervical side

4 points: If there is a bacterial film on the tooth surface 1/3 to 2/3 or less

5 points | pieces: When there is a bacterial film on the tooth surface more than 2/3 of the cervical side

Comparative example Example 1 Example 2 Example 3 Example 4 Example 5 Pre-teeth baseline 2.52 + 0.39 2.54 + 0.28 2.52 + 0.28 2.5 + 0.20 2.49 + 0.25 2.52 + 0.33 4 Weeks 2.43 + 0.32 2.30 + 0.21 2.27 + 0.28 2.22 + 0.29 2.16 + 0.27 2.23 + 0.39 % Reduction 3.57 9.28 10.05 11.48 13.03 11.58 incisor baseline 2.47 + 0.38 2.49 + 0.31 2.52 + 0.30 2.48 + 0.49 2.50 + 0.19 2.53 + 0.34 4 Weeks 2.27 + 0.37 2.23 + 0.29 2.12 + 0.22 2.14 + 0.38 2.10 + 0.26 2.15 + 0.27 % Reduction 8.09 10.36 15.96 13.82 15.99 15.05 molar baseline 2.54 + 0.51 2.56 + 0.42 2.57 + 0.25 2.57 + 0.36 2.58 + 0.41 2.54 + 0.44 4 Weeks 2.55 + 0.32 2.39 + 0.44 2.41 + 0.53 2.39 + 0.44 0.28 + 0.30 2.35 + 0.45 % Reduction 0.03 6.66 6.18 6.99 7.73 7.56 Interdental baseline 2.48 + 0.49 2.38 + 0.50 2.41 + 0.21 2.45 + 0.20 2.42 + 0.30 2.43 + 0.18 4 Weeks 2.26 + 0.42 2.12 + 0.41 2.02 + 0.37 2.11 + 0.36 2.02 + 0.40 2.04 + 0.24 % Reduction 8.87 11.09 15.99 13.89 16.47 16.08

As shown in Table 2, initially, there was no statistical significance between the comparative examples and the purification experiment groups of Examples 1 to 5 (p <0.05). Four weeks later, the experimental group of the comparative example had a decrease in plaque on the incisors and the interdental surface, and the experimental groups of Examples 1 to 5 significantly reduced the plaque index on the front teeth, the incisors, and the interdental surface (p < 0.05), the plaque index decreased significantly in molars. Thus, it was found that the tablets of Examples 1 to 5 inhibited the production of plaque, and in particular, the tablets of Examples 2 and 4 were more effective.

(3) bad breath removal effect

36 men and women who did not have dental caries were selected and subjected to a cross-over test. Commercial garlic powder was dispersed in water, left to stand for 24 hours, and diluted to make a Halimeter measurement value of 700 ppb or more. The diluted solution was used as a bad breath source. Garlic was diluted with 15 ml of garlic powder for 30 seconds, and after 1 minute, the degree of bad breath was measured with a halimeter. Then, the tablets of Comparative Examples and Examples 1 to 5 were dissolved in 15 ml and gargled for 30 seconds. After 30 seconds, 5 minutes, and 30 minutes after goggle, the degree of bad breath was measured by using a halimeter to determine whether or not to suppress bad breath. Table 3 below confirms the degree of bad breath inhibition after gargled with the oral wash tablet of the present invention.

division Elapsed time after gargle 30 sec 5 minutes 30 minutes Comparative example 55.7% 45.5% -5.9% Example 1 74.5% 73.2% 67.0% Example 2 78.3% 74.6% 70.4% Example 3 86.2% 83.5% 75.3% Example 4 88.6% 84.0% 74.2% Example 5 81.3% 78.9% 71.0%

As shown in Table 3, the tablets of the comparative example showed a slight persistence of bad breath suppression until 5 minutes after gargle, but showed an increase in the degree of bad breath after 30 minutes after gargle. On the other hand, it was confirmed that the tablets of Examples 1 to 5 have persistent persistence.

The tablet-type mouthwash composition of the present invention maintains the activity of the enzyme stably, excellent in plaque removal, bad breath removal and bad breath occurrence inhibitory effect, because it is manufactured in a tablet form, it is easy to use and easy to carry.

Claims (2)

In the tablet-type mouthwash composition, from the group consisting of glucose oxidase, dextranase, lactoperoxidase, gluconase, lysozyme, protease Tablet type mouthwash composition comprising 0.01 to 50% by weight of one or more selected enzymes and 0.1 to 9% by weight of a dehumidifying agent. According to claim 1, wherein the mouthwash composition is a glucose oxidase (dextrinase), dextranase (lactranperase), lactoperoxidase (lactoperoxidase), gluconase (gluconase), lysozyme, protease (protease) 0.01 to 50% by weight of at least one enzyme selected from the group consisting of, 0.1 to 9% by weight of the dehumidifying agent, 1 to 55% by weight of the blowing agent, 0.1 to 5% by weight of the pH adjuster, 1 to 5% by weight of the foaming agent, 0.1 to 10% by weight of the binder , 0.05-3% by weight of lubricant, 0.1-15% by weight of flavor, 0.1-15% by weight of solvent, and 0.001-10% by weight of agonist.