KR20090002682A - Cosmetic composition for preventing of kin trouble - Google Patents

Abstract

An anti-inflammation cosmetic composition, and a method for preparing an anti-inflammation cosmetic material are provided to enhance the anti-inflammation effect and the safety to the skin. An anti-inflammation cosmetic composition contains the fermentation extract which is obtained by fermenting the mushroom fungi of at least one selected from Ligusticum officinale, Lonicera japonica Thunberg, Rehmannia glutinosa, Schizonepeta tenuifolia var. japonica and Astragalus membranaceus with yeast, lactobacillus and bifidus, and extracting it, as an active ingredient.

Description

Cosmetic composition for skin trouble relief {Cosmetic composition for preventing of kin trouble}

The present invention relates to a cosmetic composition for alleviating skin troubles, and more particularly to a cosmetic composition for alleviating skin troubles while having an excellent anti-inflammatory effect, which is a skin trouble alleviating effect.

Cosmetics are products used to protect the skin and to beautify and cleanse the skin, but the composition inevitably includes components that are different from the purpose of protecting the skin. Such components include surfactants, preservatives, fragrances, sunscreens, pigments, as well as various components for imparting other effects and effects. These ingredients are generally known to cause various problems such as inflammation, rashes and edema in the skin (Maibach. H. I., Contact Dermatitis, 6. 369-404, 1980). In addition, sebum and sweat discharged from the body, fatty acids in the cosmetic ingredients, higher alcohols, protein components, etc. are decomposed into a highly toxic substance by the skin flora bacteria present on the skin may cause skin inflammation by these, It is well known that skin irritation is caused by ultraviolet rays from the sun.

Inflammatory reactions are manifested in five phenomena: feeling of redness, pricking feeling, burning hotness, swelling, and tissue changes, which can be harmful to the surrounding environment, or to external conditions such as bacteria. It is a physiological reaction to protect the living body from invasion and mechanical damage. These inflammatory phenomena result in a large increase in various types of multinucleated leukocytes (PMNs) and immune substances, and these increased cells can be treated and defended by releasing various types of proteolytic enzymes and cytokines, which are inflammatory cell products. To make it possible. However, this action can also cause detrimental damage to adjacent tissue cells and noncellular components. Thus, under appropriate conditions, normal function is restored after the initial stage of inflammation, but if the irritant that stimulates inflammation is not lost or continues to be produced, chronic inflammation will result, resulting in more serious tissue damage.

Atopy and acne, which are forms of skin problems related to inflammation, are described as follows.

Atopic dermatitis is characterized by severe itching, and when it is irritated to the skin, itching becomes more severe, and if you scratch it more and more, it causes a wound, which causes bacterial infections (yellow staphylococci, streptococci, etc.) and inflammation. One of the important factors in the problem of atopy is the secondary infection and superantigen toxin that occurs at this time. Eighty to ninety percent of patients with atopic dermatitis have Staphyococcus Aureus and Streptococcus Pyogenes infections on the skin, which produce Anit-Staphilococcus IgE, which is a superantigen toxin that stimulates T-cell responses. It is caused by and worsens. The superantigen toxin of Staphylococcus aureus selectively stimulates T lymphocytes through Langerhans intracellular antigen-presenting cells to produce interleukin-1 and TNF-alpha, and the intracellular cytokines in the T-cells stimulated by them. Secretion of toxins will cause inflammation. That is, the superantigen toxin promotes the secretion of interleukin-4 and interleukin-5, inhibits the secretion of IFN-r, increases the number of CLA, and eventually promotes the production of IgE.

Secondly, patients infected with Staphylococcus aureus secrete histamine from basophils by IgE antibodies against Staphylococcus toxin, which aggravates atopic dermatitis.

Therefore, the use of moisturizers to prevent dry skin is often recommended, and secondary infection and the control of the resulting superantigen factor is very important. In other words, atopy sees genetic defects as a basic etiology, but it is triggered by various external acquired factors.

Atopic deterioration factors include genetic factors, sensitive and dry skin, metabolic factors, excessive production of IgE, dysregulation of the autonomic nervous system, psychological factors, and stimulating factors.

As such, atopy is a disease in which the mechanism is never simple. From an immunological point of view, there is a problem of various patterns, not an immunological problem. For example, the problem of gamma interferon, which inhibits excessive production of IgE, occurs even below its mean, but may be smaller than its counterpart, interleukin-4, and the production process is problematic, resulting in incomplete production of interferon. In some cases, even if normal in terms of quantity and quality, the sensitivity to interferon stimulation may occur.

Acne, on the other hand, can be divided into inflammatory acne lesions and non-inflammatory (sclerosic) acne lesions according to symptoms.

Inflammatory acne lesions cause the sebaceous material to escape from the scalp and cause an inflammatory response in the surrounding skin tissue. Squeezing or pressing the acne swelling can cause the scalp to break down into inflammatory acne lesions, increasing the risk of acne scarring.

Basically, there are three types of inflammatory acne lesions: papules, pustules, and nodules.

Papular acne In medical terms, small bumps are called papules, which are small, hard-colored lesions that can sometimes appear as intermediate forms of non-inflammatory acne and acne lesions. . So the protruding acne is called papular acne. As mentioned earlier, this is an inflamed acne that varies in size, hardness, and severity depending on the degree of inflammation. The more severe the inflammation, the bigger and more flaky and tend to last. Less inflamed ones will only subside in very few (approximately 7%), but most often become more inflamed or pus acne.

Pulmonary acne (a pus acne) is as follows. A pus house is a battlefield after a fierce battle. In other words, a few germs, dead tissue and white blood cells to remove the germs are gathered so that the yellow pus is visible. Pustules are small and round in size, similar to papules, but they contain pus inside. There are no bacteria such as bacteria in the farm, and the inflammatory reaction is caused by chemical stimulation by components such as free fatty acids in sebum. Small, less-hard surroundings of pus acne disappear faster than larger, harder ones.

Next, nodular acne (lump acne) is the most severe form of acne, which is large, heavily inflamed and contains a large amount of pus. The nodule forms the pus by the contents of the scrim come out and induce an immune response to the surrounding skin tissue. Nodular acne is relatively deep in the skin and can be painful and is more likely to leave acne scars during the healing process than other acne lesions.

A key substance in these various inflammation-producing processes is Arachidonic acid, which is converted from cell membrane lipids by an enzyme called phospholipase A2 (PLA2) activated by stimulation, and arachidonic acid, in turn, is a cyclooxygenase (Cyclooxygenase). (Hereinafter abbreviated as 'COX') or lipoxygenase through two pathways, which are metabolized to prostaglandins (Prostaglandins) and leukotrienes (mediated) to mediate various inflammatory responses (Thomas, R., Rev. Physiol.Biochem.Pharmacol., 100, 1984).

In recent years, studies on methods for reducing skin irritation and inflammation have been actively conducted, and the results of the previous studies have shown that tissues or tissues are stimulated by nerve peptides or stimuli such as substance P (Substance P) secreted from nerve cells. Various cytokines, prostaglandin E2 (abbreviated as "PGE2") formed by kinins such as bradykinin activated by plasma or by the COX pathway, are involved in edema formation and vasodilation, Therefore, anti-inflammatory agents have been suggested as neuropeptide antagonists such as substance P, bradykinin antagonists, COX inhibitors, and the like. Most of the problems in terms of stability of the use has been limited.

On the other hand, fermentation is a process of decomposing organic matter using enzymes of microorganisms. Fermentation and decay are carried out by a similar process, but when decomposition produces a substance that is useful in our lives, it is called fermentation. Therefore, fermenting the herbal medicine using microorganisms reduces the toxicity of the herbal medicine, lowers the molecular weight, facilitates digestion, or facilitates transdermal absorption when applied to the skin, thereby improving bioavailability.

In addition, herbal medicines may exhibit pharmacological effects by themselves, but the pharmacological effects may be enhanced through fermentation, or harmful ingredients may be made by the metabolism of microorganisms, thereby exerting new pharmacological effects that were not found in herbal medicines before fermentation.

Therefore, the technical problem to be achieved by the present invention is to provide a cosmetic composition for alleviating skin problems while having an excellent anti-inflammatory effect, which is a skin trouble relief effect.

In order to achieve the above technical problem, the present invention is effective fermented extract extracted by fermenting mushrooms fermented with mushrooms of yeast, lactic acid bacteria or bifidus bacteria selected from the group consisting of cheongung, gold, silver, sulfur, mold opening and Astragalus It provides a skin trouble relief cosmetic composition comprising as a component.

Hereinafter will be described in detail with respect to the skin trouble relief cosmetic composition of the present invention.

The cosmetic composition for alleviating skin problems of the present invention is a fermented extract extracted by fermenting mushroom fungi fermented with a yeast, lactic acid bacteria or bifidus bacteria of at least one selected from the group consisting of Cheongung, Geumhwa, Chihwang, Hungyeong and Astragalus. Include as.

The Ligusticum officinale is a perennial herb of the Minaraaceae. From ancient times, cholesterol, blood, tonic, and sedatives were commonly used with anemia in anemia, coldness, dysmenorrhea and gynecological diseases. Roots as well as outposts have the same therapeutic effect. In particular, because of the analgesic effect headaches should always put a cross. For cold headaches, hyunggae, early tea, and wind headaches, like chrysanthemum. The Lonicera japonica Thunberg is also called Indong, and the first flower to bloom is white, but gradually turned yellow. Gold-silver coins are small rods or funnel-shaped buds and often lip-shaped flowers. The outer surface is yellowish white or greenish white, and the longer it is stored, the darker it is. It has a peculiar smell, it tastes sweet and its nature is cold, so it's used for heat and when your chest is stuffy and thirsty. Rehmannia glutinosa is a perennial herb of Hyunsamaceae reaching 15 to 20 cm in height. In autumn, the casserole is washed off and washed with water. It is called raw jihwang, dry is called guangjihwang, and steamed nine times to dry it is called suetjihwang. It is used to protect the kidneys with blood, tonic, hemostatic, and diuretic drugs. The hedgehog ( Schizonepeta tenuifolia var. Japonica ) is a herbaceous herb, used for colds, fever, headache or sore throat. The Astragalus (Astragalus membranaceus) is a perennial herb belonging to the legume (Leguminosae), which is used for diuretic, small, tonic.

The medicinal herb can be prepared as an extract according to a conventional method. For example, the extract can be extracted with an organic solvent such as distilled water, anhydrous C1-C4 or hydrous lower alcohol, concentrated under reduced pressure, and dried to obtain a primary extract. have. Specifically, one or more medicinal herbs selected from the group consisting of Cheongung, Geumhwa, Chihwang, Hwanggeum and Astragalus, which are commercially available as medicinal herbs, are pulverized and finely pulverized, and 5 to 20 vol. An organic solvent such as four anhydrous or hydrous lower alcohols is heated at 50-100 ° C. for 1-5 hours in an extractor equipped with a reflux condenser to extract the extracts of the medicinal herbs. After filtration with a filter cloth, the residue is further extracted one or more times in the same manner, the extracts are combined and concentrated under reduced pressure, lyophilized or spray-dried to obtain a dry extract.

The medicinal herbs selected from the group consisting of Cheongung, Geumhwa, Chihwang, Hyeonggi and Astragalus obtained as above are first fermented through mushrooms. The fungus is a mushroom of medicinal mushrooms, such as chaga (Inonnotus-obliquus), Cordyceps sinensis, Paecilomyces japonica , Trichoma matsutake, phellinus linteus, Ganoderma lucidum, etc. Can be used.

Preferably, the mushroom bacteria fermentation is inoculated with 1 to 10% by weight of the mushroom bacteria in each of the medicinal herbs or slices, powdered ground powder selected from the group consisting of Cheongung, Geumhwa, Chihwang, Hyeonghyeong and Astragalus, After fermentation for 60 days at 18 to 25 ℃, humidity 60 to 70% for 60 days to extract it can be obtained mushroom fermentation.

Extraction of the mushroom fungi fermentation is 50 to 100 ℃ in an extractor equipped with a reflux cooler with an organic solvent, such as 5 to 20 volumes of water or an anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms based on the dry weight of the fermented product. Extract the extracts of the medicines by heating for ˜5 hours. Then, the filtrate was filtered with a filter cloth, and the residue was further extracted one or more times in the same manner, the extracts were combined under reduced pressure, concentrated under reduced pressure, and freeze-dried or spray-dried to obtain a dry extract.

The mushroom bacteria fermentation is then subjected to sterilization for smooth fermentation by yeast, lactic acid bacteria or bifidus bacteria. The mushroom bacteria fermentation may be added to each yeast, lactic acid bacteria or bifidus bacteria and cultured to extract the respective fermentation extracts. Preferably, the mushroom fermentation product is preferably added in an amount of 1 to 20% by weight to a medium for culturing yeast, lactic acid bacteria or bifidus bacteria.

The yeast may be used Saccharomyces cerevisiae , Schizosaccharomyces ellipsoids , Saccharomyces boulardii and the like.

Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus confusus, Lactobacillus fermentum, Lactobacillus brevis and the like can be used.

The bifidus bacteria may be Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum , Bifidobacterium infantis and the like.

Preferably, when the yeast is used as a microorganism, the yeast is inoculated into a basic medium (YM medium) containing fermented mushroom fungus of at least one kind selected from the group consisting of Cheongung, Geumhwa, Chihwang, Hungyeong and Astragalus (preferably The concentration (approximately 10 ^{5-10 7} cells / mL) was incubated for 30-120 hours while maintaining the conditions of pH 5.5-6.5, temperature 25-35 degreeC, and aeration amount 0.05-0.5 VVM. The fermentation broth may be centrifuged to remove polymer precipitates and yeast cells, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, and then freeze-dried to obtain respective herbal fermentation extracts.

When lactic acid bacteria are used as microorganisms, inoculate the lactic acid bacteria into a basic medium (MRS medium) containing mushroom fermentation products of at least one type selected from the group consisting of Cheongung, Geumgum, Chihwang, Hungyeong, and Astragalus (preferred concentration (about 10 ^{5-10 7} cells / mL), and incubate for 24 to 120 hours while maintaining the conditions of pH 6.5-7.5, temperature 35-38 ° C., and aeration rate 1 VVM. The precipitates and lactic acid bacteria are removed, and the extract is concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor and then lyophilized to obtain respective medicinal herb fermentation extracts.

When using bifidus as a microorganism, inoculate bifidus into a basic medium (BL medium) containing fermented mushroom fungus of at least one kind selected from the group consisting of Cheongung, Geum, Yeonghwang, Hwanghyeong and Astragalus (preferred concentration). (About 10 ^{5-10 7} cells / mL), and incubate for 24 to 120 hours while maintaining an anaerobic condition at pH 6.5 to 7.5, temperature 35 to 38 ° C. Then, the fermentation broth is centrifuged to polymer precipitate. To remove the bifidus cells, concentrated under reduced pressure at 60 ℃ in a distillation apparatus equipped with a cooling capacitor and then lyophilized to obtain the respective herbal fermentation extract.

In addition, it is preferable that the total amount of the fermentation extract of the medicinal herb contained in the cosmetic composition for skin trouble relief of the present invention is 0.0001 to 20% by weight, more preferably 0.01 to 10% by weight. If the content is less than 0.0001% by weight, no obvious effect can be expected.

The formulation of the cosmetic composition for alleviating skin problems of the present invention as described above is not particularly limited and may be appropriately selected as desired. For example, the cosmetic composition of the present invention may be prepared in the form of external skin ointment, softening longevity, nourishing longevity, nutrition cream, massage cream, essence, pack, emulsion, oil gel and the like. In this case, the skin external ointment may be prepared to contain 50 to 97% by weight of petrolatum and 0.1 to 5% by weight of polyoxyethylene oleyl-ether phosphate in addition to the medicinal fermentation extract of the present invention, the flexible cosmetics fermentation of the present invention In addition to the extract, it may be prepared to contain 1 to 10% by weight of polyhydric alcohols (propylene glycol, glycerin, etc.) and 0.05 to 2% by weight of surfactants (polyethylene oleyl ether, polyoxyethylene hydrogenated castor oil, etc.). In addition, nutrient cosmetics and nourishing cream is 5 to 20% by weight of oils (squalane, petrolatum, octyldodecanol, etc.) and wax components (cetanol, stearyl alcohol, beeswax, etc.) in addition to the medicinal fermentation extract of the present invention It can be prepared to contain%, essence can be prepared by containing 5 to 30% by weight of polyhydric alcohols such as glycerin, propylene glycol in addition to the medicinal fermentation extract of the present invention. Massage cream is prepared by containing 30 to 70% by weight of oil such as liquid paraffin, petrolatum, isononyl isononanoate in addition to the medicinal fermentation extract of the present invention, the pack is polyvinyl alcohol 5-20 in addition to the medicinal fermentation extract of the present invention Wash off containing 5-30% by weight of pigments such as kaolin, talc, zinc oxide and titanium dioxide in addition to the medicinal fermentation extract of the present invention in a peel-off pack or a general emulsion cosmetic containing ) Pack.

In addition, the cosmetic composition for alleviating skin troubles of the present invention is an oil, water, surfactant, moisturizer, lower alcohol, thickener, chelating agent, colorant, preservative, or fragrance which are formulated in general skin cosmetics in addition to the components described above in each formulation. Etc. can be mix | blended suitably as needed.

The cosmetic composition for skin trouble relief of the present invention has a remarkably excellent skin trouble relief effect and persistence of skin trouble relief effect compared to the case of using the yeast fermentation extract or lactic acid bacteria fermentation extract of the conventional medicine extract, and is harmless to the human body.

Hereinafter, preferred examples are provided to help understanding of the present invention, but the following examples are merely to illustrate the present invention, and the scope of the present invention is not limited to the following examples.

EXAMPLE

Example 1

200 g of each pulverized product obtained by purchasing commercially available Cheongung, Geumgum, Sieghuang, hwanggeum and Astragalus was put in 3 L of purified water or 80% methanol, and boiled for 3 hours in a reflux extractor with a cooling capacitor. Then, the filtrate was filtered through a 100 mesh filter cloth and the residue was extracted once more in the same manner. Each extract was combined and filtered with Whatman No. 2 filter paper at room temperature to remove insoluble materials, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, and then freeze-dried to dry each dry weight as shown in Table 1 below. Got. Table 1 shows the dry weight of the extract per 100 g of medicinal herbs according to the extraction solvent.

Medicine 80% methanol Purified water Cheongung 8.2 g 7.9 g Gold coins 7.5 g 6.1 g Yellow 7.7 g 6.0 g Brother-in-law 7.9 g 6.1 g Astragalus 8.1 g 7.8 g

The mushroom fermented product was solid fermented by adding 5% by weight of the mushroom bacteria to Cheongung, Geumhwa, Chihwang, Hwanggeung and Astragalus obtained using 80% methanol.

Specifically, inoculate 5% by weight of Paecilomyces japonica under constant humidity with 200 g of Cheongung, Geumgum, Diurnal, Hwangae and Astragalus under constant humidity, and fermented and dried for 60 days at 18-25 ° C and 60-70% humidity. 100 g each of the pulverized product obtained by grinding was put into 3 L of 80% methanol, and boiled and extracted for 3 hours in a reflux extractor equipped with a cooling capacitor. Then, the filtrate was filtered with 100 mesh filter cloth and the residue was extracted once more in the same manner. Each extract was combined and filtered with Whatman No. 2 filter paper at room temperature to remove insoluble matters, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, and lyophilized to obtain a final mushroom bacterial fermentation product. The dry weight of the medicinal mushroom mushroom fermented dry matter is shown in Table 2 below.

Medicine Dry Weight of Mushroom Fermentation (g) Cheongung 6.91 Gold coins 6.75 Yellow 6.78 Brother-in-law 6.69 Astragalus 6.74

The fermented fermented mushrooms were fermented by adding 5% by weight to YM medium, a basic medium for yeast Saccharomyces cerevisiae.

Specifically, Saccharomyces cerevisiae was inoculated in a yeast basal medium (YM medium) containing 5% by weight of mushroom fermentation at a concentration of about 10 ^{5-10 7} cells / mL, pH 5.5-6.5, temperature 25-35 C was incubated for 48 hours while maintaining the conditions of 0.05-0.5 VVM of airflow. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the yeast cells, and then concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, followed by lyophilization to obtain a final medicinal mushroom mushroom-yeast fermented dry matter. The dry weight of the medicinal mushroom bacteria-yeast fermented dry matter is shown in Table 3 below.

Medicine Dry Weight of Yeast Fermentation (g) Cheongung 5.79 Gold coins 5.71 Yellow 5.64 Brother-in-law 5.62 Astragalus 5.65

Example 2

Fermented mushroom fungus of Example 1 was added to MRS medium, which is a basic medium for Lactobacillus acidophilus, by 5 wt%.

Specifically, after inoculating Lactobacillus acidophilus to the lactic acid bacteria basic medium (MRS medium) containing the mushroom fermentation of Example 1 of 5% by weight at a concentration of about 10 ⁵ ~ 10 ⁷ cells / mL, pH 6.5 ~ 7.5 The mixture was incubated for 48 hours while maintaining the temperature at a temperature of 35 to 38 ° C and aeration rate 1 VVM. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the lactic acid bacteria, and then concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, followed by lyophilization to obtain a final medicinal herb mushroom-lactic acid fermentation dried product. The dry weight of the medicinal mushroom-lactic acid bacteria fermented dry matter is shown in Table 4 below.

Medicine Dry Weight of Lactic Acid Bacteria Fermentation (g) Cheongung 5.72 Gold coins 5.64 Yellow 5.66 Brother-in-law 5.73 Astragalus 5.89

Example 3

The fermented mushroom of Example 1 was added to the B medium medium Bifidobacterium longum Bifidobacterium longum in 5% by weight of fermentation.

Specifically, Bifidobacterium longum was inoculated in a lactic acid bacteria basic medium (BL medium) containing the mushroom fermentation of Example 1 of 5% by weight at a concentration of about 10 ⁵ ~ 10 ⁷ cells / mL, pH 6.5 ~ 7.5 The culture was carried out for 48 hours while maintaining the anaerobic conditions at a temperature of 35 to 38 ° C. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the bifidus bacteria, and then concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor, followed by lyophilization to obtain a final medicinal herb mushroom-bifidus fermented dried product. . The dry weight of the medicinal mushroom bacteria-bifidus fermented dry matter is shown in Table 5 below.

Medicine Dry weight of bifidus fermentation (g) Cheongung 5.65 Gold coins 5.71 Yellow 5.75 Brother-in-law 5.68 Astragalus 5.83

Example 4-10

Mushrooms-yeast fermentation extract, mushroom fungus-lactobacillus fermentation extract, mushroom fungus-bifidus fermentation extracts of Cheonggung, Geumhwa, Chihwang, Hwanggeuk and Astragalus prepared in Examples 1 to 3 were mixed as shown in Table 6 below. Is weight ratio)

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Example 9 Example 10   Cheongung Fungi-Yeast 100 10 Mushrooms-Lactobacillus 100 50 20 10 Mushrooms-Bifidobacteria 100   Gold coins Fungi-Yeast 10 Mushrooms-Lactobacillus 100 30 Mushrooms-Bifidobacteria 10    Yellow Fungi-Yeast Mushrooms-Lactobacillus 10 Mushrooms-Bifidobacteria 100 20 10   Brother-in-law Fungi-Yeast 50 10 Mushrooms-Lactobacillus 100 20 10 Mushrooms-Bifidobacteria   Astragalus Fungi-Yeast 100 10 10 Mushrooms-Lactobacillus Mushrooms-Bifidobacteria 10

Experimental Example 1.PGE2 production inhibitory effect

In order to confirm the skin trouble relieving function of yeast fermented extract of the uterus and lactic acid bacteria fermented extract of Astragalus and the anti-inflammatory effects of Examples 1 to 10, a PEG2 production inhibitory effect test was conducted. This is an experiment to determine the effect on COX-activity (Jeffrey, kh, Anglea, SW, Zoe, S., and Rhia CM Intracellular Measurement of Prostaglandin E2: Effect of Antiinflammatory Drugs on Cyclooxygenase Activity and Prostanoid Expression, Analytical Biochemistry, 1999, 271, 18-28).

Fibroblasts isolated from human epidermal tissue were passaged several times, and then 1 × 10 ⁵ were placed in 96-well plates and incubated for 24 hours. Incubated for 2 hours by replacing with a medium not containing FBS, yeast fermentation extract of the uterus and lactic acid bacteria fermentation extract of Astragalus and Examples 1 to 10 were treated at the concentration of the following Table 7, wherein indomethacin (indomethacin) also After culturing together for 1 hour and incubating with comparative bacteria, calcium ionophore A23187 (Calcium Ionophore A23187) and arachidonic acid were treated at a concentration of 50 mM and incubated at 37 ° C. for 5 minutes. Dodecyltrimethlammonium bromide was treated with 10% final concentration of 0.25% for hemolysis of cells, and the appropriate amount of supernatant was taken to quantify the amount of PGE2 using Enzyme-Linked Immunosorbent Assay (ELISA). The prostaglandin inhibitory effect of the herbal fermentation broth was determined, and the results are shown in Table 7 below.

sample Inhibition Rate (%) Control group (negative control) - Arakidonsan (Yangseong Control Group) - Indomethacin (positive control) 93.6 YM badge 11.0 MRS Badge 16.4 BL badge 13.2 Fermented Yeast Extract of Cheongung 25.8 Astragalus Lactobacillus Fermented Extract 28.4 Example 1 60.6 Example 2 64.7 Example 3 67.5 Example 4 69.7 Example 5 65.2 Example 6 72.4 Example 7 69.8 Example 8 72.1 Example 9 66.6 Example 10 67.5

As shown in Table 7, Examples 1 to 10 of the present invention exhibits a higher inhibition rate of prostaglandin (PGE2) than yeast fermentation extracts of the horoscope, or lactic acid bacteria fermentation extracts of Astragalus or basal medium to induce or inhibit the action of COX-2 enzyme. Through the anti-inflammatory effect was found to be very excellent.

The following are examples of preparing a cosmetic composition using the above embodiments according to the present invention having excellent anti-inflammatory and anti-irritant effects and excellent skin trouble relief function.

Example 11 and Comparative Example 1

Using Example 1 to prepare a skin external ointment according to the formula shown in Table 8. (Unit: wt%)

division Example 11 Comparative Example 1 Component of Example 1 10 - Diethyl sebacate 8 8 Prepayment 5 5 Polyoxyethylene oleyl ether phosphate 6 6 Sodium benzoate Quantity Quantity vaseline To 100 To 100

Example 12 and Comparative Example 2

Using Example 3, the cream was prepared according to the formulation shown in Table 9 below.

division Example 12 Comparative Example 2 Component of Example 3 1.0 - Stearic acid 15.0 15.0 Cetanol 1.0 1.0 Potassium hydroxide 0.7 0.7 glycerin 5.0 5.0 Propylene glycol 3.0 3.0 antiseptic Quantity Quantity incense Quantity Quantity Purified water To 100 To 100

Example 13 and Comparative Example 3

Using the above Example 6 was prepared in the softener according to the prescription of Table 10. (Unit is weight%)

division Example 13 Comparative Example 3 Component of Example 6 0.2 - ethanol 10.0 10.0 Polylauric acid polyoxyethylene sorbitan 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 glycerin 5.0 5.0 1,3-butylene glycol 6.0 6.0 incense Quantity Quantity Pigment Quantity Quantity Purified water To 100 To 100

Example 14 and Comparative Example 4

Using Example 8, nutritional longevity was prepared according to the formula shown in Table 11 below. (Unit is wt%.)

division Example 14 Comparative Example 4 Ingredients of Example 8 0.1 - Waselin 2.0 2.0 Sesquioleate sorbitan 0.8 0.8 Polyoxyethylene oleylethyl 1.2 1.2 Methyl paraoxybenzoate Quantity Quantity Propylene glycol 5.0 5.0 ethanol 3.2 3.2 Carboxy Vinyl Polymer 18.0 18.0 Potassium hydroxide 0.1 0.1 Pigment Quantity Quantity incense Quantity Quantity Purified water To 100 To 100

Example 15 and Comparative Example 5

Using Example 9, the pack was prepared according to the prescription shown in Table 12 below.

division Example 15 Comparative Example 5 Ingredients of Example 9 0.5 - glycerin 5.0 5.0 Propylene glycol 4.0 4.0 Polyvinyl alcohol 15.0 15.0 ethanol 8.0 8.0 Polyoxyethylene oleylethyl 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 incense Quantity Quantity Pigment Quantity Quantity Purified water To 100 To 100

Example 16 and Comparative Example 6

Using Example 10, the essence was prepared according to the formulation shown in Table 13 below. (Unit is wt%.)

division Example 16 Comparative Example 6 Ingredients of Example 10 5.0 - Propylene glycol 10.0 10.0 glycerin 10.0 10.0 Sodium hyaluronate aqueous solution (1%) 5.0 5.0 ethanol 5.0 5.0 Polyoxyethylene Cured Castor Oil 1.0 1.0 Methyl paraoxybenzoate 0.1 0.1 incense Quantity Quantity Purified water To 100 To 100

Experimental Example 2. Anti-inflammatory Effect

Evaluation method using arachidonic acid (arachidonic acid) to determine the anti-inflammatory effect of the cosmetic prepared in Example 11 (A. Crummey, GP Harper, EA Boyle and FR Mangan, Inhibitory of arachidonic acid-induced ear edema as a model for assessing topical anti-inflammatory compound.Agents and Actions, 1987, 20: 69-76).

First, 35 mice (Hairless mouse) each divided into 7 dogs, both ears are washed clean with ethanol before sample application and measured using a micrometer, the cosmetics of Example 11 and Comparative Example 1, indomethacin ( Indomethacin) was divided into a comparison group treated with 1.0% and a control group treated with only arachidonic acid to conduct experiments to determine the average thickness of the ears of the mouse. In the sample group treated with the cosmetic of Example 11, the comparative group treated with the cosmetic of Comparative Example 1 and indomethacin 1.0%, 20 μl of the sample and 20 μl of ethanol were continuously applied to the control group once daily for 4 days. Finally, after 1 hour of curing, ethanol was applied to the left ear and arachidonic acid was applied to the right ear at 2 mg / ear. After 1 hour of application, the edema of the ear was repeated three times for both ears using a micrometer. Measured. The left ear of the treated group was used as a control to determine the degree of inflammation caused by the sample itself. Anti-inflammatory effect was determined based on the degree of inhibition of edema based on the control group treated with arachidonic acid only, the results are shown in Table 14 below.

Inhibition rate was measured according to the following equation (1).

[Equation 1]

In Equation 1, A is the average thickness change of the control ear (thickness of the arachidonic acid treatment-the thickness of the arachidonic acid untreated ear), B is the average thickness change of the sample application group ears (thickness of the siro-treated sample-the thickness of the sample untreated ear) to be.

division Concentration (%) Inhibition Rate (%) Sample bacteria Example 11 - 35.8 Comparative Group 1 Comparative Example 1 - 12.9 Comparative Group 2 Indomethacin 1.0 41.7 Control Arachidonic acid 2 mg / ear -

As shown in Table 14, the cosmetics of Example 11 according to the present invention exhibited an anti-inflammatory rate of 35.8%, and these values were lower than the indomethacin, which is a substance having excellent anti-inflammatory effect as a pharmaceutical ingredient, but a cosmetic It was found to be sufficient to be used as.

The cosmetic composition for skin trouble relief according to the present invention, while safe for the skin, enhances the anti-inflammatory effect by enhancing the pharmacological effect of each medicine through fermentation, it is excellent in the skin trouble relief effect and the persistence of the effect.

Claims (11)

Skin trouble relief comprising fermented extract extracted by fermenting mushroom fungi of yeast, lactic acid bacteria or bifidus bacteria selected from the group consisting of Cheongung, Geumgumhwa, Chihwang, Hungyeong and Astragalus as an active ingredient Cosmetic composition for. The method of claim 1, Skin trouble relief cosmetic composition, characterized in that the total amount of the fermented extract content is 0.0001 to 20% by weight. The method of claim 1, The mushroom is a skin characterized in that at least one selected from the group consisting of chaga (Inonnotus-obliquus), mushroom (phellinus linteus), Cordyceps sinensis (Cordyceps sinensis, Paecilomyces japonica ), and Ganoderma lucidum Trouble relief cosmetic composition. The method of claim 1, The yeast is one or more selected from the group consisting of Saccharomyces cerevisiae , Schizosaccharomyces ellipsoids and Saccharomyces boulardii skin cosmetic relief composition. The method of claim 1, The lactic acid bacteria Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri and Lactobacillus confusus, Lactobacillus fermentum, Lactobacillus brevis characterized by at least one cosmetic composition selected from the group consisting of a cosmetic composition. The method of claim 1, The bifidus bacteria Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum and Bifidobacterium infantis is characterized in that at least one selected from the group consisting of skin trouble relief cosmetic composition. The method of claim 1, The cosmetic composition for alleviating skin problems is a cosmetic composition for alleviating skin problems, characterized in that the external cosmetic ointment, supple cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, pack, emulsion, or oil gel formulation. The method of claim 1, The cosmetic composition further comprises one or more additives selected from the group consisting of oils, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, and fragrances. Composition. a) extracting mushroom fungi fermenting mushrooms by fermenting a medicinal herb selected from the group consisting of Cheongung, Geumgumhwa, Chihwang, Hyeonggi and Astragalus; b) fermenting the mushroom fermented product of step a) with yeast, lactic acid bacteria or bifidus bacteria; And c) separating the fermentation extract from the fermentation of step b) Method for producing a skin trouble relief cosmetic raw material comprising a. The method of claim 9, The method of producing a cosmetic ingredient for skin trouble relief characterized in that the fermentation of the mushroom bacteria step a) is a solid fermentation. The method of claim 9, Fermentation of yeast, lactic acid bacteria or bifidus bacteria of step b) is a method of producing a cosmetic raw material for skin trouble relief characterized in that the liquid fermentation.