KR20080056554A - Skin whitening composition containing ethyl acetate fraction of white ginseng with high whitening activity - Google Patents

Abstract

A skin whitening composition comprising an ethyl acetate fraction of white ginseng is provided to show high whitening activity with less cytotoxicity and be safe to human skin. A skin whitening composition comprises an ethyl acetate fraction of white ginseng as an effective ingredient, wherein the fraction is prepared by a method comprising steps of: (a) after crushing white ginseng, extracting it with a mixture solvent of methanol and distilled water; (b) filtering the extract and concentrating the filtrate under reduced pressure; (c) dividing the concentrate into an ethyl acetate soluble fraction and a water soluble fraction; and (d) after isolating the ethyl acetate soluble fraction, concentrating it under reduced pressure. A skin whitening cosmetic composition comprises the skin whitening composition as an effective ingredient. Further, the skin whiten composition comprises a cinnamic acid as an effective component.

Description

Skin Whitening Composition Containing Ethyl Acetate Fraction of White Ginseng with High Whitening Activity}

Figure 1 shows the extraction process of ethyl acetate fraction of ginseng samples.

2 is a TLC chromatogram of quercetin, maltol, p-coumaric acid, esculletin and cinnamic acid as whitening active ingredients known to be contained in ginseng samples and ginseng.

Figures 3a to f show the HPLC chromatogram of ginseng samples.

4 is a graph showing the effect of inhibiting tyrosinase activity for each whitening active ingredient and ginseng samples.

5a to 5g shows the cell growth rate and the melanin content of the group treated with only the solvent containing no sample after 100%, for the cell growth rate and melanin production in the Melanoma B16 cell line, koji acid, PTU, cinnamic acid, It is a graph which shows the effect of each compound of p-coumaric acid, esculletin, maltone, and quercetin.

Figure 6a ~ e shows the effect of each ginseng EA fraction on the cell growth rate and melanin production in Melanoma B16 cells after setting the cell survival rate and melanin content of the group treated with only the solvent containing no sample to 100% It is a graph. 6a shows the effect of dried ginseng fuselage, FIG. 6b shows the effect of dried ginseng, FIG. 6c shows the effect of dried leaves, FIG. 6d shows the effect of white ginseng, and FIG. 6e shows the effect of red ginseng.

Figure 7 shows a picture of Melanoma B16 cell line when treated for 3 days at 1ppm, 10ppm, 100ppm Kojisan, Cinnamic acid and skin white ginseng sample concentration, respectively.

FIG. 8a shows the UV absorption of 10 ppm each of cinnamic acid, quercetin, p-coumaric acid and kojic acid, and FIG. 8b shows UV absorption of ginseng samples.

Figures 9a and 9b shows the change in pigmentation after application of the fully concentrated skin white ginseng ethyl acetate fraction of the brown guinea pig skin pigmented with UV-B, Figure 9a shows after 6 weeks of application of 70% ethanol, Figure 9b shows the skin The photograph is shown after 6 weeks of application of 70% ethanol containing white ginseng sample.

The present invention relates to a skin whitening composition containing skin white ginseng ethyl acetate fraction having excellent whitening activity, and more particularly, to a composition for skin whitening containing skin white ginseng ethyl acetate fraction containing cinnamic acid as an active ingredient.

Melanin is produced against the damage of the skin caused by UV rays and protects the skin. The biosynthesis process of melanin begins with the oxidation of tyrosine by tyrosinase, the production of DOPA and DOPAchrome, etc., followed by DHICA and DHI. It is known that three species such as eumelanin (DHICA-eumelanin) and pheomelanin (Pheomelanin) are produced. Blackening of the skin is caused by melanin produced in melanocytes (melanosite: melanocyte) present in the skin cells are delivered to keratinocytes (keratinocytes: keratinocyte) and accumulated in the skin epidermal layer.

Melanin overdeposition of the skin causes blemishes, freckles, and black spots, and Kojiic acid, arbutin, and vitamin C derivatives are used to prevent and improve blackening of the skin. Although it was developed as a whitening agent, it has recently been reported that kojic acid has less melanin inhibitory effect on cellular or In Vivo than on tyrosinase on In Vitro, and may cause liver cancer in long-term use. In addition, arbutin has a problem of cytotoxicity of the hydroquinone series, and vitamin C also has a problem of chemical stability, making it difficult to store for a long time. Therefore, it is urgent to develop an excellent alternative whitening agent for this.

As side effects of whitening active substances are announced, research on functional whitening materials using various natural product extracts has been actively conducted as alternative raw materials, but whitening skin, natural extract, yulpi, and green beans are used as whitening raw materials. Clear verification is not enough.

Ginseng (人蔘, Panax ginseng CA Meyer) has been reported anti-cancer effect, immune function enhancement, anti-aging effect, anti-stress effect, anti-fatigue effect and related to the skin of keratinocytes (keratinocyte: keratinocyte) Anti-aging studies such as proliferation have been studied, but melanin production inhibitory effects on melanocytes (melanocytes) have not been reported. However, literature studies have found that ginseng contains tyrosinase inhibitors, such as esculetin, maltol, p-coumaric acid, etc. On the ground, camphorol (kaempferol), linoleic acid (linoleic acid) and the like.

       Esculetin p-coumaric acid

         Quercetin Maltol

            Cinnamic acid

Currently, several functional beauty products containing extracts of ginseng and wild ginseng cultured root are commercially available, but the tendency to use the symbolic image of Korean ginseng is greater than the actual effect, and its clinical beauty effect has not been verified yet.

In addition, there is no report on the whitening activity of ginseng extract itself, which may be due to the fact that the activity was not strong in the ginseng extract. Therefore, there is a need for derivation of components and fractions with high melanin production inhibitory activity through proper processing of ginseng and for securing whitening raw materials through improvement thereof.

Accordingly, the present inventors have completed the present invention as a result of diligent effort to develop a method for obtaining a fraction containing a high whitening activity from ginseng.

After all, an object of the present invention is to provide a method for obtaining a fraction containing a high whitening activity from ginseng and a composition for skin whitening containing the fraction.

The present invention to achieve the above object

Provided is a composition for skin whitening containing at least one organic solvent fraction selected from the group consisting of lower alkyl acetate fractions having 1 to 4 carbon atoms, ether fractions or lower ether fractions having 1 to 4 carbon atoms as an active ingredient. Preferably ethyl acetate fraction, ethyl ether fraction or ether fraction is possible, with the ether acetate fraction being most preferred.

White ginseng is usually prepared by removing the outer skin of ginseng and drying, but skin white ginseng is dry without removing the outer skin of ginseng.

In the present invention, the organic solvent fraction of skin white ginseng refers to an organic solvent soluble fraction obtained after mixing the extract of skin white ginseng with an organic solvent and a water solvent.

Here, the extract is preferably an extract extracted with 50 ~ 90% alcohol / distilled water mixed solvent. The alcohol is preferably a lower alcohol having 1 to 4 carbon atoms.

The extract is preferably ultrasonically extracted by repeating 2 to 5 for 1 to 2 hours at a temperature of 18 ~ 28 ℃.

The organic solvent fraction of the skin white ginseng is preferably used in a freeze-dried state after concentration under reduced pressure. Decompression concentration conditions can be generally used as long as the conditions used in the art, for example, decompression concentration can be concentrated for about 1 hour so as to completely evaporate the solvent using a rotary decompression concentrator below 50 ℃.

In order to achieve another object of the present invention

a) crushing the skin white ginseng sample and extracting it using a 50-90% alcohol / distilled water mixed solvent; b) filtering the extract and concentrating under reduced pressure; c) dividing the concentrated extract into an ethyl acetate soluble fraction and a water soluble fraction; And d) separating the ethyl acetate soluble fraction and concentrating under reduced pressure to obtain an ethyl acetate fraction of skin white ginseng. A method for obtaining an ethyl acetate fraction of skin white ginseng according to the present invention is schematically illustrated in FIG. 1.

In the method for separating the ethyl acetate fraction of ginseng sample, the 50-90% alcohol / distilled water mixed solvent of step a) is preferably 80% methanol / distilled water mixed solvent, and the extraction is repeated 2-5 times using ultrasonic extraction. It is preferable to extract.

The ginseng extract sample obtained in step a) was filtered under reduced pressure using whatman filter paper, and then concentrated under reduced pressure at 40 ° C to completely remove the solvent. After the prepared ginseng concentrate sample was dissolved in tertiary distilled water, ethyl acetate was added in the same amount as the water volume, mixed for 1 to 2 minutes, and the ethyl acetate layer was separated using a separatory funnel. Separation of the ethyl acetate layer is preferably repeated 2 to 4 times. The obtained ethyl acetate layer was concentrated under reduced pressure at 40 ° C. to completely remove the ethyl acetate, dissolved in a small amount of water, and lyophilized to prepare an ethyl acetate fraction.

The composition for skin whitening of the present invention may be characterized by containing cinnamic acid as an active ingredient.

The present invention also provides a skin whitening cosmetic composition containing the composition for skin whitening.

Since the EA (ethyl acetate) fraction of skin white ginseng according to the present invention contains a large amount of cinnamic acid as an active ingredient, it has excellent tyrosinase inhibitory activity, melanin inhibitory activity in melanogenesis cell line, UV-protective effect, and guinea pig skin. It can be used as an excellent whitening material because it shows pigmentation inhibitory activity at the same time.

Through the following examples will be described the present invention in more detail. These examples are only for illustrating the present invention, and the scope of the present invention is not to be construed as being limited by these examples.

Example  One:

1) Preparation of Ginseng Sample

Collected 5 kg of fresh ginseng from the ginseng farm in Chungnam Geumsan in late March 2005, partially removed the epidermis, washed it with distilled water, and divided it into the body, rice and leaf parts, and dried it in hot air at 50 ℃ for 36 hours to make white ginseng sample. It was. White ginseng for the comparative experiment with the prepared ginseng sample was used by purchasing skin white ginseng prepared in 2004 (purchased in Seoul Gyeongdong market, 4 years old, grade 1, 15 roots, Chungnam Geumsan-gun, Nonghyup Central Ginseng Inspection Office). . In addition, red ginseng was manufactured in 2004 and purchased from Jeonggwanjang. Skin white ginseng refers to dry ginseng without removing the epidermis of ginseng, and there is no research on the difference between the ingredients and efficacy of skin ginseng and white ginseng. Saponins such as ginsenosides Rg1 and Rb1 are known as main components of white ginseng, and they also contain polyacetylene and polyphenol-based substances. The main effects of white ginseng have been reported anti-cancer effect, blood circulation, memory enhancement effect.

Table 1 shows the weight for each part of the dried ginseng obtained from 5 kg of fresh ginseng by the above method.

Main roots Root hairs Leaves Total   Weight (g) 900.6 180.1 15.0 1090.7

2): of ginseng sample Fraction  Produce

Extraction of the sample was performed after grinding the body, dried ginseng, leaves and skin white ginseng and red ginseng (hereinafter referred to as 'ginseng sample') of dried ginseng (hereinafter referred to as 'dry ginseng') obtained in Example 1-1). Ultrasonic extraction was carried out three times for 1 hour at room temperature using a mixed solvent. The obtained extract was filtered through filter paper and concentrated completely using a rotary vacuum concentrator to prepare an extract. The obtained extract was then divided into ethyl acetate (hereinafter referred to as "EA") soluble fraction and water soluble fraction. First, the prepared fully concentrated 80% methanol / distilled water mixed solvent extract was dissolved in 500 ml of tertiary distilled water, and then the same amount of ethyl acetate was added and shaken for 1 to 2 minutes and left in a separatory funnel. When the ethyl acetate layer and the water layer are divided, the ethyl acetate layer is separated and repeated 2-4 times to collect the ethyl acetate layer. The collected ethyl acetate fractions were concentrated under reduced pressure and dried in vacuo to remove ethyl acetate as a solvent, and the obtained residue was dissolved in a small amount of water and lyophilized to complete the preparation of the ethyl acetate fraction sample. (See Figure 1)

Experimental Example  1: ginseng sample Yield Check  And ingredient identification

The yield of the extract obtained in Example 1 was calculated, the extraction yield of each sample is shown in Table 2.

sample Yield (80% methanol extraction) Yield (EA Fraction Extraction)  Main roots of dried ginseng 26.1% 1.31%  Root hair of dried ginseng 42.7% 2.32% Leaves of dried ginseng 39.0% 3.82%  Skin white ginseng 26.5% 0.23%  Red ginseng 31.2% 2.63%

Each figure represents% by dry weight.

As a result of Table 2, the yield of the leaves of dried ginseng was found to be the highest.

The EA fraction and the water fraction were identified by TLC (developing solvent: EA / Hexane = 1/1, 3 developments), respectively. 2A and 2B are whitening active ingredients known to be contained in ginseng, respectively, and show TLC chromatograms of quercetin, maltol, p-coumaric acid, esculletin and cinnamic acid and TLC chromatograms of ginseng samples of Table 2 above. It is shown. In FIG. 2A A: Quercetin B: Maltol C: p-coumaric acid D: Esculetin E: Cinnamic acid F: Reference mixture G: Dry body (EA fraction) H: Dry body (water fraction) I: Dry rice (EA Fraction) J: dry rice (water fraction) K: dry leaf (EA fraction) L: dry leaf (water fraction), in FIG. 2b M: dry fuselage (EA fraction) N: dry fuselage (water fraction) O: skin White ginseng (EA fraction) P: Skin white ginseng (water fraction) Q: Red ginseng (EA fraction) R: Red ginseng (water fraction).

The dried ginseng fuselage and the ginseng showed similar patterns, but the leaves showed different patterns, and the dried ginseng and white ginseng showed similar patterns according to the types of ginseng. Although, red ginseng showed a different pattern from dried ginseng or white ginseng.

In addition, when the ginseng samples were fractionated, the whitening active ingredient was transferred to the EA soluble fraction, and the whitening active ingredient was hardly transferred to the water soluble fraction.

In addition, it can be seen that the EA-soluble fractions of dried ginseng fuselage, rice ginseng, and skin white ginseng contain similar ingredients, that is, whitening active ingredients.

      Experimental Example 2: Analysis of the active ingredient content for each ginseng sample

The content of dry ginseng EA fractions of quercetin, maltol, cinnamic acid, p-coumaric acid and esculletin, the whitening active substance identified in the EA fraction, was quantified using HPLC.

As a sample solution, the Ginseng EA fraction was prepared by dissolving the fully concentrated extract in methanol at 10 mg / ml and filtering with a 0.45 μm syringe filter (Millipore), which was used as a sample for analysis. HPLC was performed by Jasco Co. Analytical LC (liquid chromatography) from (Japan) was used. The column used Bondpack C18 (4 um, 300 × 3.9 mm) and the mobile phase was gradientd at 0.8 mL / min by gradient of water with 2% acetic acid and 50% acetonitrile with 0.5% acetic acid. Eluted. The temperature of the column was maintained at 40 ℃ and the detection of the sample was measured at 280 nm. All samples were averaged by three replicates and co-injection with the standard was used to confirm peak agreement.

3 a to f show HPLC chromatograms of various ginseng samples. FIG. 3a shows the peaks of Maltol, Esculetin (Esc), Coumaric acid (Cou), Cinnamic acid (Cin) and Quercetin (Que) as reference materials, and FIG. 3b shows the peaks of dried ginseng bodies. 3c confirms the peak of dried ginseng, FIG. 3d confirms the peak of dried ginseng leaf, FIG. 3e confirms the peak of skin white ginseng, and FIG. 3f confirms the peak of red ginseng.

Referring to FIG. 3, reference materials quercetin, maltol, cinnamic acid, p-coumaric acid, and esculinate are dissolved in methanol and eluted under conditions described in the method described above through ODS-H80 reverse phase chromatography. , At 9.2, 13.6, 19.7, 33.8, and 34.9 minutes, respectively.

The whitening active substance content of each part of the dried Ginseng EA fraction is shown in Table 3, and the results of measuring the contents of dried Ginseng, skin White Ginseng and Red Ginseng are shown in Table 4.

sample Esculetin p -coumaric acid Sinnamsan Quercetin Maltol  fuselage 0.10% 1.52% 0.10% 0.01 or less 0.01 or less Misam 0.09% 0.33% 0.05% 0.01 or less 0.01 or less  leaf 0.64% 0.26% 0.20% 0.16% 0.01 or less

sample Esculetin p -coumaric acid Sinnamsan Quercetin Maltol Dried ginseng 0.10% 1.52% 0.10% 0.01 or less 0.01 or less Skin white ginseng 0.01 or less 0.19% 1.51% 0.24% 0.01 or less Red ginseng 0.96% 0.07% 0.06% 0.01 or less 3.82%

As shown in Table 3 and Table 4, the parts of dried ginseng contained 1.52% of p-coumaric acid in the fuselage, showing a large difference from 0.33% of rice ginseng, and the highest content of esculletin was 0.64% in leaves. there was. Skin white ginseng contained 1.51% cinnamic acid and p-coumaric acid contained less than dry ginseng. In addition, red ginseng contained a large amount of maltol, a specific component of red ginseng, but relatively less other ingredients.

Example 3: Confirming the whitening activity for each whitening active ingredient and ginseng sample

1) How to measure whitening activity

end. How to measure tyrosinase activity inhibition

8.0 mM L-dopa (dissolved in 67 mM phosphate buffer (PH 6.8)) in a 96-well microplate with various concentrations of 120 μl dissolved in 40 μl methanol and tyrosinase (125 U / ml). 40 μl was added. After incubation at 37 ° C. for 20 minutes, the amount of dopachrome produced was measured at 492 nm using an Elisa reader.

I. Melanin-producing cell line test

Melanogenic cell lines were cultured in RPMI1640 medium containing 10% FBS (Fetal Bovine Serum). After inoculating a cell suspension containing approximately 5 × 10 5 cells in a 100 π culture dish and growing confluent at 37 ° C. and 5% CO 2, 105 cells / well were placed on a 24-well plate. Inoculated again at concentration and incubated for 24 hours. 990 μl of medium per well was changed daily, and 10 μl of the sample was treated for 3 days (solvent: 50% propylene glycol (30% EtOH, 20% H 2 O), followed by further incubation for 1 day.

In order to determine the effect on melanin production, the medium was washed with phosphate-buffered saline (saline, phosphate-buffered: PBS) and immediately dissolved with 1 N NaOH 1 ml to measure melanin and UV absorbance was measured at 400 nm.

To determine the effect on cell viability, medium was removed, washed with PBS, and 200 μl of crystal violet (0.1% crystal violet, 10% EtOH, remaining PBS) was added. After incubation at room temperature for 5 minutes, it was washed twice with water. 1 ml of EtOH was added thereto and shaken at room temperature for 10 minutes, and then UV absorbance was measured at 590 nm.

2) by sample Whitening activity  Confirm

end. Whitening Active Ingredient  Inhibition of Tyrosinase Activity by Ginseng and Ginseng Samples

As a result of measuring the effects on the tyrosinase activity, an enzyme that plays a major role in the initial melanogenesis process by each sample using the method described in 1) is shown in FIG.

Among the whitening substances, esculletin showed the best inhibitory activity of tyrosinase at 4 ppm at 200 ppm, and quercetin and cinnamic acid showed 36.7% and 34.5% inhibition at the same concentration, respectively. EA fraction of ginseng samples showed inhibitory activity of 12.3% and 13.7% at 200 ppm concentration in dry body and skin white ginseng, respectively. This can be said to have a significant inhibitory activity considering the relatively low activity at the same concentration compared to a single active substance such as esculletin, but the extract is a mixture of several components. In Figure 4, when the dried leaves, especially 20ppm, the tyrosinase inhibitory activity was slightly better than the skin white ginseng, but the UV-blocking effect or activity in the melanin-producing cells showed that the skin white ginseng showed excellent results, which was excellent in skin white ginseng. It can be seen that.

I. Inhibition of melanin production in melanocytes

To determine the effect of whitening actives and ginseng samples on cell viability and melanogenesis in Melanoma B16 cell line, a mouse-derived melanin-producing cell (Korea Cell Line Bank), 10% fetal bovine serum (FBS) and 1% penicillin-strepto A control sample dissolved in EtOH / H ₂ O = 6/4 solution incubated at 37 ° C and 5% CO ₂ in RPMI 1640 medium containing mycin, 200 nM Phorbol-12 myristate 13-acetate (PMA) ug / ml, 10 ug / ml, 100 ug / ml were treated for 3 days.

Figure 5 shows the effect of each compound on the cell growth rate and melanin production of melanoma B16 cell line when the survival rate and the content of melanin carrier is initially set to 100%, koji acid as a control, PTU as a positive control, cinnamic acid Graphs of p-coumaric acid, esculletin, maltol and quercetin are shown in order, respectively.

According to FIG. 5, PTU (Phenyl thiourea), a positive control, showed cytotoxicity of about 25% at 100 ug / ml, but decreased melanin production rates 11.1%, 58.6%, and 74.7%, respectively, in a concentration-dependent manner. Kojiic acid, used as a control, showed cytotoxicity at high concentrations. Among the components of ginseng, cinnamic acid was 17% at 10 ug / ml and 29 at 100 ug / ml without high cytotoxicity. The production of melanin was reduced in%, and esculletin showed melanin reduction compared to cell viability at a concentration of 100 ug / ml, but caused slight cell death. Other maltol, p-coumaric acid and quercetin showed no significant effect.

Figure 6 is a graph showing the effect of each ginseng EA fraction on the cell growth rate and melanin production of melanoma B16 cell line when the survival rate and the content of melanin carrier is initially set to 100%. 6a shows the effect of dried ginseng fuselage, FIG. 6b shows the effect of dried ginseng, FIG. 6c shows the effect of dried leaves, FIG. 6d shows the effect of white ginseng, and FIG. 6e shows the effect of red ginseng.

Ginseng samples showed melanin reduction of about 30% of melanin at 100 ug / ml at 100 ug / ml concentration and EA fraction of leaves at 27 ug / ml at 27 ug / ml. The EA fraction and the red ginseng EA fraction and the red ginseng EA fraction showed high cytotoxicity.

In summary, the EA fraction of skin white ginseng showed the least toxicity and the most excellent activity among ginseng samples, and the cytotoxicity was low and the melanin reduction effect was excellent. Since the fraction was confirmed to contain a large amount of cinnamic acid in Example 2) 2, it could be determined that cinnamic acid plays a major role in the melanin inhibitory activity of skin white ginseng.

Based on this, the melnoma B16 cell line when treated with kojic acid, cinnamic acid, and skin white ginseng samples was identified through photographs to find the basis for the above judgment.

Figure 7 shows melanoma B16 cell photo when treated with kojisan, cinnamic acid and skin white ginseng sample concentration at 1ppm, 10ppm, 100ppm respectively for 3 days. Please describe the interpretation of the data for FIG. Kojic acid, the standardizing whitening activator for comparison of activity, did not show significant cell death in all 1ppm, 10ppm, and 100ppm treatment groups, but did not significantly reduce the amount of melanin produced in black. In contrast, skin white ginseng EA and cinnamic acid reduced melanin production in a concentration-dependent manner without significant cell death.

3) Check UV-A and UV-B area UV protection effect

UV-A (350 ~ 370 nm) of the ultraviolet region has a long wavelength, can penetrate the dermal layer of the skin and cause immediate pigmentation in the ultraviolet region that can pass through the glass. UV-B (270-290 nm), on the other hand, has a shorter wavelength than UV-A, so it does not penetrate deep into the skin, but its high energy causes sunburn and delayed pigmentation, which occurs after 72 hours of exposure.

Ultraviolet absorbance measurement of the sample was carried out by measuring the UV absorbance at 200 nm ~ 600 nm by dissolving the test sample in 10 ppm in methanol.

Figure 8 shows the UV absorption of each sample in the UV-A and UV-B region. The absorbance in the 200-500 nm region measured the absorption of 100 g / ml of skin white ginseng EA fraction in the UV-B region and the red ginseng EA fraction showed weak absorption in the UV-B region. Leaves and fuselage did not show absorption in the UV-B and UV-A regions. Among the components of ginseng, cinnamic acid showed the most characteristic absorption in the UV-B region, and quercetin showed some absorption in the UV-A and UV-B regions.

4) brown Guinea Pig  Used Skin Pigmentation  Check for mitigation effect

After shaving the back of 500 grams of brown guinea pig females, anesthetize and induce pigmentation by irradiating 500 mJ UV-B to the same site three times a week. Each test sample solution was applied to the site once a day for 6 weeks and the degree of pigmentation relaxation was measured.

9 shows the pigmentation change of the skin of the brown guinea pig pigmented with UV-B. Pigmentation was induced on the skin of Brown guinea pigs with UV-B, and the skin white ginseng EA fraction was dissolved in 70% ethanol and applied for 6 weeks. It was confirmed that the relaxation.

Hereinafter, various formulations that can be prepared using the skin white ginseng EA fraction having the least cytotoxicity and good whitening activity in the present invention as a whitening composition. However, the scope of the present invention is not limited to the following formulation examples will be apparent to those skilled in the art.

Formulation example

Formulation Example 1 Nutritious Longevity (Milk Lotion)

A whitening nutrient lotion containing skin white ginseng EA fraction was prepared according to a conventional method as shown in the following table.

Ingredient weight% Skin White Ginseng EA Fraction 2.0 Squalane 5.0 Beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Caprylic / Capric Triglycerides 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity Purified water to 100

Formulation Example 2 Softener (Skin Lotion)

A softening lotion containing skin white ginseng EA fraction was prepared according to a conventional method as shown in the following table.

Ingredient weight% Skin White Ginseng EA Fraction 2.0 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxy Vinyl Polymer 0.1 PEG 12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, flavoring Quantity Purified water to 100

[ Formulation example  3] Nutrition Cream

A nourishing cream containing skin white ginseng EA fraction was prepared according to a conventional method as shown in the following table.

Ingredient weight% Skin White Ginseng EA Fraction 2.0 Polysorbate 60 1.5 Solbitan Sesquioleate 0.5 PEG60 Cured Castor Oil 2.0 Liquid paraffin 10.0 Squalane 5.0 Caprylic / Caprol Triglycerides 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 antiseptic Quantity Pigment Quantity Spices Quantity Purified water to 100

[ Formulation example  4] pack

A pack containing the skin ginseng EA fraction was prepared according to a conventional method as shown in the following table.

Ingredient weight% Skin White Ginseng EA Fraction 1.0 Polyvinyl alcohol 13.0 Sodium Carboxymethyl Cellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 PEG 12 nonylphenyl ether 0.3 Polysorbate 60 0.3 Preservative, coloring, flavoring Quantity Purified water to 100

As described in detail above, the present invention can provide a whitening composition containing skin white ginseng ethyl acetate fraction with high whitening activity and low cytotoxicity. In addition, the skin whitening cosmetics using the skin white ginseng ethyl acetate fraction according to the present invention as an active ingredient can be used as a functional cosmetic for skin whitening because it can provide safety and whitening effect on human skin. .

Claims (10)

Skin whitening composition comprising the ethyl acetate fraction of skin white ginseng as an active ingredient. The skin whitening composition according to claim 1, wherein the ethyl acetate fraction of skin white ginseng is an ethyl acetate soluble fraction obtained after mixing the extract of skin white ginseng with ethyl acetate and a water solvent. The skin whitening composition according to claim 2, wherein the extract of skin white ginseng is an extract extracted with a 50-90% alcohol / distilled water mixed solvent. The composition for skin whitening according to claim 3, wherein the alcohol is methanol. The composition for skin whitening according to claim 3, wherein the extract of skin white ginseng is ultrasonically extracted by repeating 1 to 2 hours at a temperature of 18 to 28 ° C. for 2 to 5 hours. The composition for skin whitening according to claim 1, wherein the ethyl acetate fraction of skin white ginseng is used in a freeze-dried state after concentration under reduced pressure. The method of claim 1, wherein the ethyl acetate fraction of skin white ginseng a) grinding the skin white ginseng sample and extracting the mixture using a methanol / distilled water mixed solvent; b) filtering the extract and concentrating under reduced pressure; c) dividing the concentrated extract into an ethyl acetate soluble fraction and a water soluble fraction; And d) separating the ethyl acetate soluble fraction and concentrating under reduced pressure to obtain an ethyl acetate fraction of skin white ginseng. The composition for skin whitening according to claim 1, wherein the composition contains cinnamic acid as an active ingredient. A skin whitening cosmetic composition comprising the composition for skin whitening according to any one of claims 1 to 8 as an active ingredient. a) grinding the skin white ginseng sample and extracting the mixture using a methanol / distilled water mixed solvent; b) filtering the extract and concentrating under reduced pressure; c) dividing the concentrated extract into an ethyl acetate soluble fraction and a water soluble fraction; And d) separating the ethyl acetate soluble fraction and concentrating under reduced pressure to obtain an ethyl acetate fraction of skin white ginseng.

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