KR20080044100A - Animal feed composition containing by-products obtained from purification process of the conjugated linoleic acid - Google Patents

Abstract

An animal feed composition containing the purification by-products of conjugated linoleic acid which are discarded as an active ingredient is provided to efficiently produce an animal and animal products containing highly unsaturated fatty acid at low costs. An animal feed composition contains 0.02 to 10 parts by weight of the purification by-products of conjugated linoleic acid and additionally dietary supplements, food additives and/or feed additives, based on the total weight of the composition. The purification by-products of conjugated linoleic acid are obtained by purifying conjugated linoleic acid, adding the filtrate and removing hexane. The animal is a cow, horse, mule, donkey, goat, sheep, deer, pig, rabbit, dog and poultry. The poultry is a chicken, turkey, duck, ostrich, goose or quail.

Description

Animal feed composition containing conjugated linoleic acid refined by-products {Animal Feed Composition containing by-products obtained from purification process of the conjugated linoleic acid}

1 is a schematic diagram of a method for preparing a conjugated linoleic acid purified by-product of the present invention through the synthesis and purification of conjugated linoleic acid (CLA).

Figure 2 is a graph confirming the thermal stability of the conjugated linoleic acid purified by-products. CLA represents a conjugated linoleic acid, CBP represents a conjugated linoleic acid purified byproduct, LA represents a linoleic acid, and LBP represents a linoleic acid purified byproduct, which is the same below.

Figure 3 is a graph showing the results of the peroxide value of the conjugated linoleic acid purified byproducts (peroxide value, POV test).

Figure 4 is a photograph confirming the degree of esterification of various fatty acids contained in the conjugated linoleic acid purified by-product by TLC. Mono-, di- and tri-linoleic acid were used as standard samples.

Figure 5 is a graph confirming the accumulation of the conjugated linoleic acid in egg yolk of the egg produced by the laying hen after feeding the feed composition containing the conjugated linoleic acid purified by-product of the present invention to the laying hen. As an internal standard, margaric acid (Heptadecanoic acid) was used.

Figure 6 is a graph confirming the time-phase content change of cholesterol in egg yolk of the egg calculated by the laying hen after feeding the feed composition containing the conjugated linoleic acid purified byproducts of the present invention.

The present invention relates to an animal feed composition containing a conjugated linoleic acid purified by-product of polyunsaturated fatty acid as an active ingredient.

In recent years, various adult diseases are increasing rapidly due to the improvement of the income of the modern man and the change of diet, and vascular disease and cancer are recognized as a threat to the health of the modern man. In connection with these diseases, various functional raw materials have been identified and various studies have been actively conducted. As a result, various functional raw materials useful for the human body have been developed, and various functional foods containing these ingredients have been commercialized.

Among them, conjugated linoleic acid, a polyunsaturated fatty acid having 18 or more carbon atoms, has been found to be involved in defense mechanisms against arteriosclerosis, immune strengthening, and anticancer in animal experiments. Active research is ongoing (Ha, YL et al. 1990, Cancer Res . 50: 1097; Belury, MA et al . 1996, Nutr . Cancer 26: 149; Ip, C. et al. 1991, Cancer Res . 50: 6118; Liew, C. et al. 1996, Carcinogenesis 16: 3037), although the protective effects of various adult diseases have been reported as described above, there have been no studies on the improvement of stability and the increase of effective body accumulation. On the other hand, the effects of omega-3 fatty acids consisting of three or more double bonds have already been demonstrated in various studies through the body stability and increased stability. Omega-3 polyunsaturated fatty acids are found to be mainly contained in the meat of fish, and active research is being conducted since it has emerged as a major concern in Western societies based on meat with high saturated fatty acid content.

Poultry is a livestock with relatively little alteration of fatty acids by fatty acid metabolism in the body, and in addition to linoleic acid, linolenic acid, type 3 PG (prostagladin) and type 5 Omega-3 polyunsaturated fatty acids such as eicosapentaenoic acid (EPA), a precursor of leukotriene, and DHA (docosahexaenoic acid), an omega-3-fatty acid in fish oil, are known to have high accumulation efficiency.

In the past, there have been many studies for accumulating and fortifying functional polyunsaturated fatty acids in livestock, especially poultry and poultry-derived livestock products, using fatty acids as dietary additives. Since these prior arts use high-purity fatty acids as raw materials, enormous costs are required when refining raw materials, and the by-products produced during oil refining also contain large amounts of functional fatty acids. Disposal of the entire product without seeking it failed to utilize its value. In addition, the disposal costs of the purification by-products were also incurred.

In addition, when the high-purity fatty acid is in the form of free fatty acid, the conversion of glycerin into triglyceride, a fatty acid ester of glycerin, for absorption of fat in the animal body is slow. The accumulation of functional components in poultry-derived livestock products was not obtained, and the accumulation rate was not high. In addition, the feed of the animal feed of conjugated linoleic acid has a problem such as lowering the feed intake of animals to reduce the productivity and quality of livestock of poultry-derived livestock.

Accordingly, the present inventors have made diligent efforts to develop feed compositions for accumulating and strengthening functional polyunsaturated fatty acids in livestock products at low prices, and as a result, a large amount of conjugated linoleic acid esterified in the conjugated linoleic acid purified by-products that have been completely discarded is obtained. The present invention was completed by confirming that it was contained, and applying it as a feed composition of livestock to obtain a functional livestock enriched with conjugated linoleic acid quickly and efficiently without degrading the productivity and quality of poultry or poultry derived livestock. .

An object of the present invention to provide an animal feed composition containing a conjugated linoleic acid purified by-product as an active ingredient.

Another object of the present invention is to provide a livestock product of a livestock which is bred with the feed composition of the present invention and is effective in anti-obesity, arteriosclerosis prevention, and anti-cancer.

Still another object of the present invention is to provide a method for producing a conjugated linoleic acid purified byproduct.

In one aspect, the present invention provides an animal feed composition containing a conjugated linoleic acid (CLA) purified byproduct as an active ingredient. Specifically, the animal feed composition of the present invention may include 0.01 to 25 parts by weight, preferably 0.02 to 10 parts by weight of the conjugated linoleic acid purified byproducts based on 100 parts by weight of the total composition.

The term "conjugated linoleic acid purified by-product" of the present invention is purified from conjugated linoleic acid (crude CLA) synthesized by a known conjugated linoleic acid synthesis method, and then recovered from the filtrate. It contains a large amount of esterified conjugated linoleic acid.

In the embodiment of the present invention, an efficient preparation method and stability of the conjugated linoleic acid purified by-products have been identified, and conjugated linoleic acid having a physiological activity efficiently without sacrificing productivity and quality of poultry meat or poultry-derived livestock products. It was confirmed that the livestock product with enhanced conjugated linoleic acid could be prepared by imparting the functionality of. Therefore, in one aspect, the present invention provides a livestock product of the livestock breeding with the feed composition of the present invention is effective in anti-obesity, arteriosclerosis prevention, anti-cancer.

Animals in the present invention, as defined in Article 2 No. 1 of the Livestock Act and Article 2 of the Enforcement Rule of the Act, may be livestock that are suitable for breeding and are able to contribute to the income increase of farms. The livestock may be cattle, horses, mules, donkeys, goats, goats, sheep, deer, pigs, rabbits, dogs, poultry, etc., poultry chickens, turkeys, ducks, ostrich, goose, quail, etc., preferably chicken However, if the breeding is suitable for obtaining livestock products are not limited thereto. "Livestock" means the meat, milk, eggs, honey and processed products thereof, raw hides (including raw hides), raw wool and other livestock products, as defined in Article 2, paragraph 3 of the Livestock Act, as prescribed by the Ministry of Agriculture and Forestry; Means that.

As described in detail in the following examples, the feed composition of the present invention has high thermal stability and oxidative stability, high esterification degree of functional polyunsaturated fatty acids, and thus is suitable as a feed composition. Polyunsaturated fatty acid is accumulated at high efficiency without any change in cholesterol level in the living body.

Therefore, in one aspect, the present invention provides a livestock product of the animal having the anti-obesity, arteriosclerosis, anti-cancer effect by dietary intake of the feed composition of the present invention. In a specific embodiment, the livestock product of the present invention may be meat, milk, or eggs produced from livestock bred as a feed composition containing the conjugated linoleic acid purified by-product of the present invention as an active ingredient, but is derived from livestock as a second method of livestock production. It does not limit to livestock products of Article 3;

The term " feed " of the present invention means any natural or artificial diet, one meal, or the like or any ingredient of the one meal for the animal to eat, ingest and digest. In one embodiment, the feed composition containing the conjugated linoleic acid purified by-product of the present invention as an active ingredient may include a thick feed, a forage and / or special feed.

The rich feed includes seed fruits containing grains such as wheat, oats and corn, and by-products obtained by refining grains, bran, beans, fluids, sesame seeds, linseed, coco, etc., including rice bran, bran and barley bran. Concentrated fresh liquid from fish, fish meal, fish residues and other fish residues such as by-products such as dried starch, sweet potato, and potato. Animal feeds such as fish soluble, meat meal, blood meal, milk powder, skim milk powder, milk from cheese, and whey, which is the balance used to make cardine in skim milk , Yeast, chlorella, and seaweed.

Forage, raw vegetables such as grasses, grasses, and green grass, fodder turnips, fodder beets, and root vegetables such as Lutherbearger, a type of turnip, raw grass, green crops, and grains Silage (silage), grasses, grasses, and dried hay, straws of breeder crops, and leaves of legumes. Special feeds are supplemented with mineral feeds such as oyster shells and rock salts, urea feeds such as urea and its derivatives, diureide isobutane, and natural feed ingredients. Feed additives, which are added in small amounts.

As a specific aspect, the present invention is a by-product of corn, soybean meal (corn gluten meal), by-products produced during the production of corn starch (corn gluten meal), vitamin complexes, limestone (limestone), etc. It provides an animal feed composition containing. In addition, the animal feed composition of the present invention may further comprise a dietary supplement, food additives or feed additives.

A "dietary adjuvant" is a formulation containing a composition that provides a therapeutic or digestive agent to an animal, for example, and refers to a composition that is ingested in addition to a conventional animal feed, usually not a caloric intake source, ie, an energy source. In addition, "food additive" refers to the intentionally added in small amounts to the food for the purpose of improving the appearance, flavor, tissue or shelf life, improve the quality of the food, not only improve the preservation or palatability, but also the nutritional value and It is meant to be used for the purpose of enhancing the actual value of food. As defined in Article 2, Paragraph 2 of the Food Sanitation Act, this may be a substance used for adding, mixing, infiltrating, or otherwise using the food in manufacturing, processing or preserving the food. In addition, the "feed additive" refers to a substance added to the feed for various effects such as supplementation of nutrients and weight loss prevention, improving the digestive availability of the fiber in the feed, improving the oil quality, preventing reproductive disorders and improving the conception rate, prevention of high temperature stress in summer. The feed additive of the present invention corresponds to a feed supplement under the Feed Control Act, and minerals such as sodium bicarbonate, bentonite, magnesium oxide, composite minerals, minerals such as trace minerals such as zinc, copper, cobalt, and selenium. Preparations, vitamins such as keratin, vitamin E, vitamins A, D, E, nicotinic acid, and vitamin B complexes, protective amino acids such as methionine and lyric acid, protective fatty acids such as fatty acid calcium salts, probiotics (lactic acid bacteria), yeast culture It may be a live bacteria, yeast, such as water, mold fermentation products.

The conjugated linoleic acid purified by-products included in the feed composition of the present invention can be recovered from the remaining filtrate after purifying conjugated linoleic acid after synthesizing conjugated linoleic acid (crude CLA). Specifically, as described in detail in Example 1 to be described later, by reacting ethylene glycol and KOH, and then by adding safflower oil to the reaction further to prepare a conjugated linoleic acid (crude CLA), to which hexane is added And then recovered, and then purified by decontamination and decolorization process by urea clathrate to purify the conjugated linoleic acid, the purified conjugated linoleic acid is separated and the remaining filtrate is dissolved in water, and then hexane is added and removed To produce conjugated linoleic acid purified byproducts. The method for purifying conjugated linoleic acid may be modified to include addition steps or modification steps known in the art, in addition to the above method, and the present invention is to separate conjugated linoleic acid from the filtrate and to remove the conjugated linoleic acid. Purified by-products are obtained and used. Preferably, the weight ratio of the conjugated linoleic acid: urea: MeOH is 1: 3: 9, and the weight ratio of filtrate: water is 1: 2 for dissolving the residual filtrate in water.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, these examples are merely illustrative of the present invention and thus the present invention is not limited.

Example 1 Preparation of Conjugated Linoleic Acid (CLA) Purified By-Product

Example  1-1: Conjugated linoleic acid  ( crude CLA Synthesis and Purification

Conjugated linoleic acid (hereinafter, abbreviated as "CLA") purified by-product of the present invention was prepared by the following method. First, 150 g of ethylene glycol and 22.5 g of KOH are reacted at 200 ° C. for 15 minutes, and 40 g of safflower oil is added to the reaction solution, followed by stirring at 200 ° C. for 90 minutes to give a conjugated linoleic acid (crude). CLA) was prepared. At this time, nitrogen gas was slowly flowed into the reactants during the reaction from the step of adding safflower oil to prevent rancidity caused by fatty acid air. Thereafter, the reaction was cooled to 80 ° C. at room temperature, 60 g of HCl was added thereto, followed by further stirring for 30 minutes, and then the separated CLA layer was recovered. In recovering the CLA layer, a small amount of hexane was added to make the separation of layers appear clearly. Hexane contained in the recovered crude CLA was all evaporated by nitrogen concentration method.

Example  1-2: CLA  Recovery of Purified By-Products

CLA purified byproducts were recovered from crude CLA prepared and recovered by the method of Example 1-1 through purification and decolorization by urea inclusion. It was most effective to use the weight ratio of crude CLA: urea: MeOH as 1: 3: 9 used in this example.

First, add MeOH and Urea (urea, CO (NH ₂ ) ₂ ) to a Double Jacketed Reactor, keeping the temperature constant at 70 ° C. Then, until all the elements are dissolved and the color of the reactant is clear. The stirring reaction was carried out. Thereafter, crude CLA prepared and recovered by the method of Example 1-1 was added to the reaction solution, followed by further stirring for 10 minutes, and then cooled to 0.2 ° C / min from 70 ° C to 5 ° C. . After cooling of the reaction solution, the crystallized reactant contained in urea was filtered under reduced pressure while washing with hexane. The vacuum filtered crystallization was used to obtain purified CLA. The remaining filtrate was dissolved in water in the ratio of filtrate: water = 1: 2, and then a small amount of hydrochloric acid (HCl) was added thereto, followed by stirring for 10 minutes. Hexane was added to the reaction solution and stirred for 5 minutes. After recovering the supernatant therefrom, hexane was distilled off, and residual CLA purified byproducts were obtained for use as feed composition of livestock (FIG. 1).

From the above results, a method for efficiently preparing conjugated linoleic acid purified by-products that has not been utilized in the food and feed industry has been proposed.

Example 2 Stability Analysis of Four Types of Fatty Acids

Example  2-1: Thermal stability  analysis

The thermal stability of four types of fatty acids (CLA, CLA purified byproducts, linoleic acid, linoleic acid purified byproducts) used in the present invention was verified by the following method.

In order to confirm the degree of relative thermal stability, samples of the four fatty acids were heat-treated in an oven at 95 ° C. for 96 hours, and samples were taken at each time interval, which was determined by gas chromatography (hereinafter referred to as “GC”). Abbreviated as) to compare the change of each fatty acid content.

As a result of observing the concentration change of the four types of fatty acids for 96 hours, as shown in FIG. 2, CLA was decreased by 26.47% and linoleic acid was 30.08%, but CLA purified byproducts were 2.07% and linoleic acid purified byproducts were 15.62. % Disappeared. From the above results, it was confirmed that each of the purified byproducts had a relatively high thermal stability compared to the purified CLA and linoleic acid (FIG. 2).

Example  2-2: Peroxide Value Measurement peroxide value , POV test )

Gwasanhwamulga measurement results may be used as an indicator of the stability and oxidative damage to the fatty acid, POV test is the content of peroxide present in the fatty acid spectrophotometric method (Raghavan, S. and Hultin, HO , J. Agric. Food Chem ., 53: 4572-4577, 2005). The larger the POV, the stronger the toxicity, but since the content of hydoperoxide decreases after reaching the maximum value with the progress of autoxidation, as shown in the result after 24 hours in FIG. The peroxide value of the sample that has undergone excessive automatic oxidation may be surprisingly low.

As a result of the POV measurement, the peroxide value of CLA reached the highest point between 12 hours and 24 hours at 95 ° C. The CLA purified by-products also showed the same trends over time, but the change was relatively smaller than that of CLA. In addition, the linoleic acid purified by-products also did not have a large POV rise compared to linoleic acid (FIG. 3). From the above results, it was confirmed that the conjugated linoleic acid purified by-product of the present invention was more stable against oxidative damage as compared with the purified conjugated linoleic acid.

<Example 3> Degree of esterification of functional polyunsaturated fatty acid

The degree of esterification of the four types of highly functional polyunsaturated fatty acids (CLA, CLA purified byproducts, linoleic acid, linoleic acid purified byproducts) used in the present invention is as follows. It was confirmed by the method.

A solution of 80 ul of each fatty acid diluted with 0.7 ml of hexane was taken as a fixed size point on a water-free TLC plate (silica gel), and then hexane (diethylether): methanol (methanol): acetic acid ( Acetic acid) (80: 15: 3: 2, v / v) was used to absorb the developing solvent onto the plates in a TLC bath. After a certain time, the plate was taken out and completely dried, and then 10% Molybdatophosphoric acid (MPA) was evenly sprayed on the plate. Thereafter, a little heat was applied to the plate to confirm the distribution of glycerin (glyceride) of each fatty acid band (FIG. 4).

From the above results, it was confirmed that the form of CLA mainly distributed in the oil by-product is esterified form, and the form of CLA mainly distributed in the purified oil is free fatty acid form. The esterified form of fatty acids is more readily absorbed in the gut than the free fatty acid form, and can quickly form a micelle structure, which can result in more efficient body accumulation when fed with animal feed. From the above results, it was confirmed that the CLA purified by-product of the present invention can be used as a feed composition of livestock.

<Example 4> Preparation of feed composition containing CLA purified by-products

The composition of the purified by-product-containing feed composition for strengthening conjugated linoleic acid according to the present invention is shown in the following table.

 Control CLA Tablet Byproducts CLA Ingredient (% )   Corn  57.0 53.0 53.0   Soybean meal  18.0 18.0 18.0   Corn gluten meal   5.0  5.0  5.0   bran   9.7 11.7 11.7   Salt   0.3  0.3  0.3   Limestone   7.5  7.5  7.5 Vitamin-Premix ¹   0.5  0.5  0.5   L-Lysine   0.5  0.5  0.5   Methionine   0.5  0.5  0.5   Bicalcium phosphate   1.0  1.0  1.0   Oil byproduct -  2.0 -   CLA oil - -  2.0 Chemical composition (Chemical composition (%) )   Dry matter   Ash  9.13 8.08 8.00   Crude protein 14.46 14.76 14.62   Ether extract   Crude fiber   3.35 3.66 3.31   Calcium   Phosphate   ME (Kcal / kg) 3,456 3,622 3,609

Compositions provided per Kg of feed of superscript ¹ in the table are as follows: Vitamin A, 5,500 IU; Vitamin D _{3 ,} 1,100 IU; Vitamin E, 11 IU; Vitamin B ₁₂ , 0.0066 mg; Riboflavin, 4.4 mg; Niacin, 44 mg; Pantothenic acid, 11 mg; Calcium-pantothenic acid (Ca-pantothenate), 11.96 mg; Choline, 190.96 mg; Choline chloride, 220 mg; Menadione, 1.1 mg menadione sodium bisulfite complex, 3.33 mg; Folic acid, 0.55 mg; Pyridoxine, 2.2 mg; Pyridoxine hydrochloride, 2.67 mg; Biotin, 0.11 mg; Thiamin, 2.2 mg; Thiamine mononitrate, 2.40 mg; Ethoxyquin, 125 mg; Cu, 30; Zn, 60; Mn, 90; Co, 0.25; I, 1.2.

<Example 5> Accumulation degree of functional polyunsaturated fatty acid in egg yolk

CLA purified by-product of the present invention was fed dietary feed of animals, and then the degree of accumulation of functional polyunsaturated fatty acids in egg yolk was confirmed by the following method.

Example  4-1. Egg yolk  Extraction of my fat

The control group using a normal laying hen diet and the two groups using a laying hen diet with functional polyunsaturated fatty acids (CLA, CLA purified byproducts) (2% each) were divided into 48 groups and white leghon. Feeding experiments were conducted for 5 weeks under conditions. During the experiment, feed intake, egg produntion rate, and egg quality were measured for each group, and only egg yolks were collected from the egg samples collected daily and stored at -20 ° C. To compare the accumulation of functional polyunsaturated fatty acids in egg yolk at different time periods, only fat was extracted from yolk samples collected weekly using Hexane: Isopropanol (3: 2, v / v) solvent.

Example  4-2. Extracted fat methyl Esterification

The extracted fat was subjected to methyl esterification in the following manner.

First, toluene and toluene (5% KOH) were added to the sample extracted only from the egg yolk sample and reacted at 70 ° C. for 8 minutes and then cooled. The same temperature was added by adding 14% BF3 (boron trifluoride). After reacting for 8 minutes at, it was cooled. Thereafter, saturated NaCl and hexane were added to recover the methyl esterified fatty acid as hexane, which was then analyzed by GC. For objective analysis, all experiments were repeated three times, and margaric acid (Heptadecanoic acid) was used as an internal standard to calculate the recovery of fat per gram of egg yolk.

As a result of confirming the accumulation of functional polyunsaturated fatty acids and egg quality in egg yolk by the above method, the group fed the dietary diet containing CLA refined by-product fed the dietary composition containing purified CLA (CLA 80%) Compared with the group, the CLA content in egg yolk was significantly accumulated during the 5-week breeding experiment. In addition, CLA accumulation in egg yolk was observed after 3 weeks of feeding in the purified CLA addition group, whereas CLA refined by-product addition group showed a high accumulation rate of 5% or more after 1 week of feeding (Fig. 5).

Table 2 shows the effects of laying hen diets on the quality change of eggs of feed compositions containing conjugated linoleic acid purified by-products. From the results, it was confirmed that the group fed the feed composition containing the conjugated linoleic acid purified by-product did not show a significant difference from the quality of eggs in the group fed the normal feed (Table 2).

  Quality parameters Dietary groups SEM p -value Control CLA Tablet Byproducts CLA   Feed Intake (g / d) 102.333 100.628 99.611 11.544 0.4053   Laying rate (%) 82.13 82.74 79.12 14.439 0.4232   Egg Weight (g / egg) 62.52 ^ab 61.99 ^b 64.12 ^a 3.292 0.0487 Eggshell Strength (g / m ² ) 2844.5 2906.3 2600.0 750.9 0.3531   Eggshell thickness (mm) 0.411 0.416 0.393 0.061 0.3067   Egg Yolk Color 8.929 8.971 8.757 0.821 0.3417   Haugh unit 85.68 ^a 81.16 ^b 80.14 ^b 7.978 0.0109   Egg Yolk Height (mm) 18.63 ^a 18.53 ^a 18.22 ^ab 1.060 0.0341   Egg Yolk Diameter (mm) 43.13 ^a 42.10 ^b 43.59 ^a 1.541 0.0010   Egg Yolk Index 0.433 ^ab 0.441 ^a 0.419 ^b 0.033 0.0123

In the above table, other superscripts ^{a, b, c, and d} in the same row mean a significant difference. ( p <0.05)

<Example 6> Measurement of cholesterol in egg yolk

After dietary supplementation of CLA purified by-products of the present invention to white leg horn breeding hens, cholesterol content in yolk was confirmed by the following method.

The yolk samples collected weekly during the experiment in Example 4-1 were stored at -20 ° C, which were taken out immediately before analysis and analyzed by enzymatic digestion using a cholesterol kit (cholesterol kit, Wako). Cholesterol levels in yolk of the group fed CLA tablet byproducts prepared in Example 4 increased with cholesterol levels in the control group in the first week after dietary supplementation, but soon stabilized to show no significant differences between dietary groups. (Figure 6).

From the above description, those skilled in the art can understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features. In this regard, it should be understood that the examples and experimental examples described above are exemplary in all respects and not restrictive. The scope of the present invention should be construed to encompass the meaning and scope of the following claims rather than the foregoing detailed description, and all changes or modifications derived from the equivalent concept thereof are included in the scope of the present invention.

The present invention provides an animal feed composition containing a conjugated linoleic acid purified by-product that has been completely discarded and not utilized in the food and feed industry to produce livestock products effective against anti-obesity, arteriosclerosis, and anti-cancer. It is effective to provide. In addition, by using the feed composition containing the conjugated linoleic acid purified by-product of the present invention, it is possible to efficiently produce livestock and livestock-derived livestock containing polyunsaturated fatty acids as an active ingredient at low cost, thereby preventing and preventing various adult diseases at low cost. Accumulation of functional ingredients and consequent enhancement of the body's physiological activity will increase the health of consumers.

Claims (10)

Animal feed composition containing a conjugated linoleic acid purified by-product as an active ingredient. The animal feed composition according to claim 1, wherein the composition comprises 0.02 to 10 parts by weight of the conjugated linoleic acid purified byproducts based on 100 parts by weight of the total composition. The animal feed composition of claim 1, wherein the composition further comprises a dietary supplement, a food additive, and / or a feed additive. The animal feed composition according to claim 1, wherein the conjugated linoleic acid purified byproduct is obtained after purifying and separating the conjugated linoleic acid and adding and removing hexane to the remaining filtrate. The animal feed according to any one of claims 1 to 4, wherein the animal is any one selected from the group consisting of cattle, horses, mules, donkeys, goats, goats, sheep, deer, pigs, rabbits, dogs and poultry. Composition. 6. The animal feed composition of claim 5, wherein the poultry is chicken, turkey, duck, ostrich, goose or quail. A method for obtaining livestock products with increased polyunsaturated fatty acid content using the feed composition of claim 1. 8. The method of claim 7, wherein the livestock product is meat, milk or eggs. (a) preparing conjugated linoleic acid, purifying and separating the remaining filtrate, and dissolving the remaining filtrate in water, followed by reaction with hydrochloric acid and hexane; And (b) recovering the supernatant from the reaction solution to remove hexane. The method of claim 9 wherein dissolving the residual filtrate of step (e) in water has a weight ratio of filtrate to water of 1: 2.

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