KR101939535B1 - Feed additives containing cyanidin-3-glucoside and method of preparing the same - Google Patents

Abstract

The present invention relates to a feed additive containing cyanidin-3-glucoside (C3G) components, in which a C3G-containing extract, extracted from a raw material selected from the group consisting of aronia, Rubus coreanus, mulberry, blueberries, and acai berries, is mixed with at least one excipient selected from the group consisting of oligosaccharides, glucose, sugar, honey, and maple syrup. The C3G component-containing feed additive of the present invention promotes the healthy skeletal formation and weight gain of livestock.

Description

[0002] Feed additives containing cyanidin-3-glucoside and method of preparing the same,

The present invention relates to a feed additive containing a C3G component and a preparation method thereof, and more particularly to a feed additive containing a C3G ingredient and a method for producing the C3G ingredient feed additive by using an extract containing C3G extracted from a raw material selected from the group consisting of Aronia, Rubus, Feed additives and a process for producing the same.

Feed additives are substances that are added to diets for nutritional or specific purposes to reduce the disease prevention and mortality of livestock, to promote the growth of livestock, to improve the digestion and absorption of nutrients, Or improving the quality of livestock products by improving the composition of livestock body tissues.

Due to the nature of the feed, even if the nutrients are included in the feed, the effect on the growth of the livestock depends on a little of the digestible nutrients. Therefore, if the fat contained in feed is decomposed into fatty acid, monoglyceride, diglyceride, glycerol, carbohydrate to glucose, protein to amino acid and peptide, It is necessary to optimally absorb and optimally utilize in the living body. In addition, nutritional balance of feed is an important factor for the success of livestock breeding, so feed additives for healthy breeding are needed.

Especially, the breeding of large broiler chicks weighing 3.5kg or more is very important to balance the skeleton that can withstand gravity due to rapid growth. Especially, the healthy development of the early pelvic skeleton is more important factor in the growth and survival of the pelvis.

Korean Patent Registration No. 10-0647067 discloses a poultry feed composition containing brambles and Korean Patent Laid-Open No. 10-2015-0116225 discloses a livestock feed additive containing a by-product of a fermented product of Omija sugar. The above-mentioned technologies have the effect of promoting the growth of livestock and improving the quality, but have limitations in achieving the desired result.

Cyanidin-3-glucoside (C3G) is an antioxidant that suppresses aging by anthocyanin contained in flowers and fruits. C3G is known to have an aging inhibitory effect 5 to 7 times stronger than tocopherol (vitamin E). C3G prevents the aging of the brain and aging of the body by removing active oxygen, and is effective in treating retinal disorders and improving vision. In addition, there is a report that the growth of tumor cells of mouse skin is delayed and the growth of human lung cancer cells and the transfusion are reduced. In other studies, it has been reported that C3G decreases serum cholesterol and triglyceride content due to the synergistic action of linoleic acid, an unsaturated fatty acid, in a hyperlipemia-induced rat experiment.

It has also been reported that C3G promotes survival and muscle differentiation of mouse mesenchymal cells. In this study, when C2C12 cells were induced to differentiate, C3G protein was highly expressed and increased in mouse skeletal muscle and increased C3G appeared in the nucleus of muscular dendritic cells (K. Sasi Kumar et al., Biochimica et biophysica acta, Molecular cell research, Vol. 1853, No. 10, pt.A, 2015, pp. 2629-2639).

Therefore, the inventors of the present invention have found that a raw material containing a large amount of C3G component having a useful effect as described above is selected, and the feed additive provided by using the C3G-containing extract extracted from the raw material is used as a raw material for the livestock Promoting growth and promoting metabolism, and reached the present invention.

Korean Patent Registration No. 10-0647067 (2006.11.10) Korean Patent Publication No. 10-2015-0116225 (Oct. 15, 2015)

 C3G (RapGEF1), a regulator of actin dynamics promoters survival and myogenic differentiation of mouse mesenchymal cells (K. Sasi Kumar et al., Biochimica et biophysica acta, Molecular cell research, 1853, 10, pt.A, 2015, p2629 ~ 2639)

It is an object of the present invention to provide a feed additive containing a C3G component.

Another object of the present invention is to provide a method for producing a feed additive containing C3G component.

According to an aspect of the present invention,

(Cyanidin-3-glucoside) -containing extract from a raw material selected from the group consisting of at least one selected from the group consisting of atorvastatin, And a C3G component feed additive which is obtained by mixing at least one excipient selected from the group consisting of at least one C3G component.

In one embodiment of the present invention, the C3G-containing extract may be 0.01 to 1.0 part by weight based on 100 parts by weight of the excipient.

In one embodiment of the present invention, the water content of the C3G-containing extract may be 13 to 17%.

In one embodiment of the present invention, the C3G-containing extract may have a size of 80 to 200 mesh.

In one embodiment of the present invention the feed additive is an animal feed animal selected from the group consisting of chickens, cows, pigs, dogs, horses, donkeys, sheep, goats, cats, rabbits, turkeys, geese, ducks and quail Lt; / RTI >

According to another aspect of the present invention,

(a) extracting cyanidin-3-glucoside (C3G) -containing extract by extracting from a raw material selected from at least one selected from the group consisting of aronia, bokbunja, audi, blueberry, and ascorbic acid; And

(b) mixing the C3G-containing extract with an excipient selected from the group consisting of oligosaccharides, glucose, sugar, honey, and maple syrup, with a C3G component-containing feed additive.

In one embodiment of the present invention, (c) further comprises drying the mixture of the C3G-containing extract prepared in the step (b) and the excipient at 40 to 60 ° C.

In one embodiment of the present invention, the C3G-containing extract in step (a) may be prepared by extracting the raw material from a solvent selected from the group consisting of water and alcohol.

In one embodiment of the present invention, the dried material dried in step (c) may be stored at 1 to 10 ° C.

The C3G ingredient-containing feed additive of the present invention helps the formation of a healthy skeleton of livestock and helps in weight gain. The feed additive containing the C3G component of the present invention can be applied to livestock such as chickens, cows, pigs, dogs, horses, donkeys, sheep, goats, cats, rabbits, turkeys, geese, ducks and quails, .

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a flow chart of the production process of the feed additive containing the C3G component of the present invention. Fig.
FIG. 2 is a photograph showing a body weight of a chicken aged 50 days after feeding the feed additive of the present invention. FIG.
FIG. 3 (a) is a photograph of the chest of 50-day-old chickens fed with the feed additive-added feed of the present invention, and FIG. 3 (b) is a photograph of the chest of 50-day old chickens fed only with general broiler feed.
FIG. 4 (a) is a photograph of the leg of a chicken aged 50 days old fed with the feed additive of the present invention, and FIG. 4 (b) is photograph of leg of a chicken aged 50 days old.
FIG. 5 is a photograph of a 50-day-old chickens fed with the feed additive-added feed of the present invention.

An embodiment of the present invention relates to a feed additive containing a C3G component, wherein an extract containing C3G extracted from a raw material selected from at least one selected from the group consisting of aronia, brambles, audi, blueberry and ascorbic acid is mixed with an oligosaccharide, , Honey, and maple syrup.

Aronia is a generic term for shrubs and their fruits that belong to the genus Rosaceae Aronia and is the highest anthocyanin content among berries. Anthocyanins contained in Aronia are known to have antioxidant activity, which not only prevents aging but also has excellent anticancer effect. It is also known to help prevent diabetes, relieve inflammation, prevent liver damage, improve vision, and lower cholesterol levels to prevent cardiovascular disease and stroke.

Bokbunja is a fruit of deciduous shrub of Rosaceae Rosaceae which is known to promote renal function. Anti-inflammatory, antioxidant and anti-Helicobacter pylori activities have been reported as pharmacological actions.

Oddi is a fruit of mulberry and is known as a tonic, and it is known to improve the function of liver and kidney. It is also said to be effective on insomnia and forgetfulness, and effective on blood sugar and cholesterol lowering, by softening the joints and relaxing the mind.

It is known that blueberry is a fruit of shrubs of azalea rhododendron of the dicotyledonous plant, promotes activation of rhodopsin re-synthesis of eye retina, capillary protection function, antioxidant activity, anti-ulcer activity, anti-inflammatory effect.

Asai Berry is a fruit of Asai palm trees grown in the Amazon rainforest region of Brazil. Especially, the bark of Asai berry contains functional anthocyanin, which is a soluble pigment of flavonoid system, and beta-sitosterol, which is a type of phytosterols. It contains antioxidant activity to remove active oxygen which is harmful to our body. It has been reported that it has anti-aging effect, cardiovascular disease and cancer prevention effect.

These excipients help to uniformly blend C3G-containing extracts and aid in promoting metabolic energy.

In the present invention, glucose may be liquid glucose, purified glucose, or hydrated crystalline glucose.

In the present invention, the oligosaccharide may be a fructooligosaccharide, an isomaltooligosaccharide, a galactooligosaccharide, a maltooligosaccharide or a xyloligosaccharide, and may be in the form of a liquid or a powder.

In the feed additive containing the C3G component of the present invention, the C3G-containing extract is preferably mixed with 0.01 to 1.0 part by weight with respect to 100 parts by weight of the excipient. More preferably, the mixing amount of C3G-containing extract is 0.015 to 0.1 part by weight with respect to 100 parts by weight of the excipient, and more preferably 0.02 to 0.08 part by weight with respect to 100 parts by weight of the excipient.

In the present invention, the C3G-containing extract may be in a liquid phase or a solid phase. In the case of a solid phase, it may be in the form of a powder or granules.

In the feed additive containing the C3G component of the present invention, the water content of the C3G-containing extract is preferably 13 to 17%. Moisture content refers to the weight of moisture occupied by the total weight of C3G-containing extract. A more preferred C3G-containing extract has a moisture content of 14.5 to 15%.

In the C3G-containing feed additive of the present invention, the C3G-containing extract preferably has a size of 80 to 200 mesh. When the size is within the above range, the feed efficiency is high and the palatability of the feed of chickens can be suitably selected. A more preferred C3G-containing extract has a size of 110 to 150 meshes.

The C3G component-containing feed additive of the present invention can be used for feeds intended for livestock. In the present invention, livestock includes chicken, cow, pig, dog, horse, donkey, sheep, goat, cat, rabbit, turkey, goose, duck, quail and the like.

The C3G component-containing feed additive of the present invention helps the skeletal formation of the livestock and the smooth propagation of the growth cell tissue. In addition, toxin factors exposed in the breeding environment can strengthen the cell defense function of livestock, remove oxidative toxin in blood, purify blood, increase leukocyte and activate it to strengthen immunity. It can also help alleviate arthritis in livestock.

In particular, the C3G component feed additives of the present invention are suitable for chicken feed and more suitable for broiler feed. Furthermore, it is suitable for raising a broiler chickens of 3.5kg or more. This is because the feed additive of the present invention helps the formation of skeleton of broiler chickens, so that a skeleton capable of withstanding the rapid growth of large broiler chickens weighing 3.5 kg or more can be provided.

The C3G-containing feed additive of the present invention may be used in an amount of 0.5 to 10 parts by weight based on 100 parts by weight of the general livestock feed, preferably 1 to 5 parts by weight.

Another embodiment of the present invention relates to a method for preparing a feed additive comprising a C3G component,

(a) extracting a C3G-containing extract from a raw material selected from at least one selected from the group consisting of aronia, bokbunja, audi, blueberry, and acai berry; And

(b) mixing the C3G-containing extract with an excipient selected from the group consisting of oligosaccharides, glucose, sugar, honey and maple syrup.

Hereinafter, the method for producing a feed additive containing a C3G component of the present invention will be described in detail. Fig. 1 shows a flow chart of the production process of the feed additive containing the C3G component of the present invention.

In describing the method for producing a feed additive containing a C3G component of the present invention, the same description as that of the C3G ingredient-containing feed additive will be omitted.

First, an extract containing C3G is prepared by extracting from a raw material selected from at least one selected from the group consisting of aronia, bokbunja, audi, blueberry and acai berry (step (a)).

In one embodiment of the present invention, the C3G-containing extract may be extracted by a hydrolysis method in a raw material selected from at least one group selected from the group consisting of Aronia, Rubus, Audi, Blueberry and Acai Berry.

In one embodiment of the present invention, the C3G-containing extract is selected from the group consisting of at least one selected from the group consisting of Aronia, Rubus, And the like. The extraction temperature is preferably 2 to 10 ° C. The extraction time is preferably 1 to 24 hours, more preferably 2 to 10 hours. Also, the ratio of the raw material to the solvent is preferably 1: 1 to 5 by weight.

Next, the C3G-containing extract is mixed with an excipient selected from the group consisting of oligosaccharides, glucose, sugar, honey and maple syrup (step (b)). At this time, it is preferable that the C3G-containing extract is mixed with 0.01 to 1.0 part by weight with respect to 100 parts by weight of the excipient. More preferably, the mixing amount of C3G-containing extract is 0.015 to 0.1 part by weight with respect to 100 parts by weight of the excipient, and more preferably 0.02 to 0.08 part by weight with respect to 100 parts by weight of the excipient.

In the method for preparing a feed additive containing a C3G component of the present invention, the method may further include a step of drying the mixture of the C3G-containing extract and the excipient prepared in the step (b) (step (c)). The drying is preferably carried out at 40 to 60 ° C. If the drying temperature is lower than 40 캜, there is a problem that the drying time becomes longer. When the drying temperature is higher than 60 캜, the color and the composition may be deteriorated. A more preferred drying temperature is 45 to 50 占 폚.

In the method for producing a feed additive containing a C3G component of the present invention, it is preferred that the dried material dried in the step (c) is stored at 1 to 10 ° C. A more preferred storage temperature is 2 to 8 占 폚.

Hereinafter, the method for producing a feed additive containing a C3G component of the present invention will be described in more detail with reference to the following examples.

<Examples> Production of C3G-containing feed additive

Aronia was washed. The water was added twice as much as the weight of the washed Aronia aerials and then extracted at 5 ° C for 3 hours using a shaker. The extract thus extracted was filtered. The amount of the filtrate thus filtered was concentrated to 1/5 and spray-dried to granulate to a size of 120 meshes to prepare a C3G-containing extract. 0.5 g of the C3G-containing extract was added to 1 kg of liquid fructooligosaccharide and mixed. The mixture of the C3G-containing extract and the oligosaccharide mixed at the above was dried at 48 DEG C to granulate to prepare a C3G-containing feed additive. The dried C3G-containing feed additive was stored at 5 &lt; 0 &gt; C.

&Lt; Experimental Example >

1) Experimental animals

One-day-old broiler chicks (Hanseong 3, native chickens) hatched from Hanshin hatchery in Muan-myeon, Milyang-si, Gyeongsangnam-do were used for the experiment.

2) Group separation (n = 10)

The experiment was divided into two groups: the experimental group fed the general compound feed supplemented with the feed additive prepared in the Examples and the control group fed only the normal compound feed.

3) Feeding

The diets were fed with broiler diets (produced at the Haman plant) produced by NACF and fed with broiler chickens <Crumble> with corn - wheat bran - soybean meal crude protein of 19.50% or more and MEn 3,050 kcal / kg before 4 weeks of age, Later, broiler chow meats <pellet> were fed with corn-soybean meal-based crude protein of 19.50% or more and MEn of 3,100 kcal / kg or more. In the experimental group, feeds containing 2% of the C3G-containing feed additive prepared in the Example were fed to the broiler-dedicated feeds. In the control group, only broiler-only feeds were provided.

4) Weight measurement

The weights of the experimental group and the control group were measured on the 20th, 30th, 40th and 50th days of the experiment using the scales, and the results are shown in Table 1.

20 days (g) 30 days (g) 40 days (g) 50 days (g) Experimental group 951 8.0 2017 ± 15.2 3020 ± 14.2 4060 + - 71.3 Control group 501 ± 9.8 930 ± 23.2 1860 + - 57.2 1970 ± 36.1

As shown in Table 1, the body weight of the experimental group was significantly increased compared to the control group. Generally, chickens raised only for broiler chickens have growth slower than 35 days, and weight gain is not much. In the control group, the average body weight was 1860 g at 40 days of age, and the body weight at 50 days was the average of 1970 g. On the contrary, in the experimental group, the body weight of 40 days old was 3020g and the body weight of 50 days old was 4060g. FIG. 2 shows a photograph of the body weight (4.07 kg) of a 50-day-old experimental group. In this way, it is not easy to raise broilers of 4 kg or more for broiler breeding, but the experimental group to which the feed additive of the present invention is fed has grown into broiler having an average body weight of 4060 g.

5) Evaluation of breasts and legs of experimental animals

On the 50th day after the experiment, the blood vessels were rapidly induced by the external vascular shear method and then dehydrated by the hot water tapping method. After removing the head, ankles and internal organs, the chest and legs were taken and visually confirmed and promoted. The color, elasticity, and glossiness were measured using the 5 point scale method (very poor: 1, poor: 2, , Very good: 5). The results are shown in Table 2.

Breast meat Leg meat color elasticity burnish color elasticity burnish Experimental group 4.6 4.7 4.8 4.3 4.5 4.5 Control group 3.2 3.0 3.0 3.4 3.3 3.2

FIG. 3 (a) shows a picture of the chest of the experimental group at 50 days of age, and FIG. 3 (b) shows the photograph of the breast of the control group at 50 days of age. FIG. 4 (a) shows a photograph of the legs of the experimental group for 50 days, and FIG. 4 (b) shows the legs of the control group after 50 days of age. Referring to FIGS. 3 and 4 and Table 2, it was confirmed that the color, elasticity, and glossiness of the breast meat and leg meat of the experimental group were much better than those of the control group.

6) Evaluation of foot of experimental animal

Fig. 5 shows photographs of the feet of the 50-day old experimental group. As can be seen from Fig. 5, the foot of the experimental group was found to be about the size of the general cigarette value, and it was confirmed that the elasticity of the flesh was excellent.

While the present invention has been described in connection with certain exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims.

Claims (9)

delete delete delete delete delete (a) Extraction of cyanidin-3-glucoside (C3G) -containing granules of particle size of 80 to 200 mesh by filtration, concentration, and spray drying to obtain an extract containing cyanidin-3-glucoside ;
(b) mixing the extract containing C3G derived from Aronia with an excipient selected from the group consisting of oligosaccharides, glucose, sugar, honey and maple syrup, wherein the C3G-containing extract is added in an amount of 0.01 to 1.0 Mixing parts by weight; And
(c) drying the mixture of the extract containing C3N derived from Aronia produced in step (b) and excipient at 45 to 50 DEG C,
Wherein the dried product obtained in step (c) is stored at 1 to 10 ° C. delete delete delete

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