KR19990073078A - Feed Compositions Containing Conjugated Linoleic Acids - Google Patents
Feed Compositions Containing Conjugated Linoleic Acids Download PDFInfo
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- KR19990073078A KR19990073078A KR1019990011775A KR19990011775A KR19990073078A KR 19990073078 A KR19990073078 A KR 19990073078A KR 1019990011775 A KR1019990011775 A KR 1019990011775A KR 19990011775 A KR19990011775 A KR 19990011775A KR 19990073078 A KR19990073078 A KR 19990073078A
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- South Korea
- Prior art keywords
- cla
- cis
- trans
- content
- feed
- Prior art date
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- 229940108924 conjugated linoleic acid Drugs 0.000 title claims abstract description 175
- 239000000203 mixture Substances 0.000 title claims abstract description 63
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 claims abstract description 194
- JBYXPOFIGCOSSB-GOJKSUSPSA-N 9-cis,11-trans-octadecadienoic acid Chemical compound CCCCCC\C=C\C=C/CCCCCCCC(O)=O JBYXPOFIGCOSSB-GOJKSUSPSA-N 0.000 claims abstract description 174
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims abstract description 34
- 235000013601 eggs Nutrition 0.000 claims abstract description 33
- 235000019197 fats Nutrition 0.000 claims abstract description 27
- 244000144972 livestock Species 0.000 claims abstract description 22
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 21
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 21
- 235000013372 meat Nutrition 0.000 claims abstract description 18
- 235000012000 cholesterol Nutrition 0.000 claims abstract description 16
- 210000002969 egg yolk Anatomy 0.000 claims abstract description 13
- 241000286209 Phasianidae Species 0.000 claims abstract description 12
- 210000003278 egg shell Anatomy 0.000 claims abstract description 12
- 235000013345 egg yolk Nutrition 0.000 claims abstract description 11
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 25
- 239000000194 fatty acid Substances 0.000 claims description 25
- 229930195729 fatty acid Natural products 0.000 claims description 25
- 150000004665 fatty acids Chemical class 0.000 claims description 24
- 238000004519 manufacturing process Methods 0.000 claims description 17
- 235000015277 pork Nutrition 0.000 claims description 17
- 241000287828 Gallus gallus Species 0.000 claims description 12
- 235000005911 diet Nutrition 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 8
- 235000013330 chicken meat Nutrition 0.000 claims description 6
- IOCYQQQCJYMWDT-UHFFFAOYSA-N (3-ethyl-2-methoxyquinolin-6-yl)-(4-methoxycyclohexyl)methanone Chemical compound C=1C=C2N=C(OC)C(CC)=CC2=CC=1C(=O)C1CCC(OC)CC1 IOCYQQQCJYMWDT-UHFFFAOYSA-N 0.000 claims description 5
- 241000272525 Anas platyrhynchos Species 0.000 claims description 5
- ADHNUPOJJCKWRT-JLXBFWJWSA-N (2e,4e)-octadeca-2,4-dienoic acid Chemical compound CCCCCCCCCCCCC\C=C\C=C\C(O)=O ADHNUPOJJCKWRT-JLXBFWJWSA-N 0.000 claims description 2
- -1 triglyceride fatty acid Chemical class 0.000 claims description 2
- 241000009328 Perro Species 0.000 claims 1
- 241000282898 Sus scrofa Species 0.000 claims 1
- 230000000378 dietary effect Effects 0.000 claims 1
- 125000005457 triglyceride group Chemical group 0.000 claims 1
- 241000282472 Canis lupus familiaris Species 0.000 abstract description 14
- 241000282887 Suidae Species 0.000 abstract description 14
- 238000007796 conventional method Methods 0.000 abstract description 7
- 150000003626 triacylglycerols Chemical class 0.000 abstract description 5
- 235000004213 low-fat Nutrition 0.000 abstract description 3
- 230000001737 promoting effect Effects 0.000 abstract description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract 2
- 241000272517 Anseriformes Species 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 235000014655 lactic acid Nutrition 0.000 abstract 1
- 239000004310 lactic acid Substances 0.000 abstract 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 30
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 27
- 241000030538 Thecla Species 0.000 description 27
- 239000003925 fat Substances 0.000 description 22
- 230000000694 effects Effects 0.000 description 21
- 239000003921 oil Substances 0.000 description 19
- 235000019198 oils Nutrition 0.000 description 19
- 239000000047 product Substances 0.000 description 16
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 15
- 235000020778 linoleic acid Nutrition 0.000 description 15
- 238000006243 chemical reaction Methods 0.000 description 11
- 235000011121 sodium hydroxide Nutrition 0.000 description 10
- 235000019485 Safflower oil Nutrition 0.000 description 9
- 240000008042 Zea mays Species 0.000 description 9
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 9
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 9
- 235000005822 corn Nutrition 0.000 description 9
- 230000037213 diet Effects 0.000 description 9
- 239000003813 safflower oil Substances 0.000 description 9
- 235000005713 safflower oil Nutrition 0.000 description 9
- 235000013343 vitamin Nutrition 0.000 description 9
- 239000011782 vitamin Substances 0.000 description 9
- 229940088594 vitamin Drugs 0.000 description 9
- 229930003231 vitamin Natural products 0.000 description 9
- 235000019764 Soybean Meal Nutrition 0.000 description 8
- 239000004455 soybean meal Substances 0.000 description 8
- 241001465754 Metazoa Species 0.000 description 7
- 238000009395 breeding Methods 0.000 description 7
- 230000001488 breeding effect Effects 0.000 description 7
- 150000003722 vitamin derivatives Chemical class 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 6
- 229910052500 inorganic mineral Inorganic materials 0.000 description 6
- 235000010755 mineral Nutrition 0.000 description 6
- 239000011707 mineral Substances 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- 241000272522 Anas Species 0.000 description 5
- 235000006439 Lemna minor Nutrition 0.000 description 5
- 244000242291 Lemna paucicostata Species 0.000 description 5
- 235000019738 Limestone Nutrition 0.000 description 5
- 235000013364 duck meat Nutrition 0.000 description 5
- 230000001976 improved effect Effects 0.000 description 5
- 239000006028 limestone Substances 0.000 description 5
- 235000013379 molasses Nutrition 0.000 description 5
- 230000002829 reductive effect Effects 0.000 description 5
- 235000019733 Fish meal Nutrition 0.000 description 4
- 239000001506 calcium phosphate Substances 0.000 description 4
- 239000008157 edible vegetable oil Substances 0.000 description 4
- 239000004467 fishmeal Substances 0.000 description 4
- 235000013336 milk Nutrition 0.000 description 4
- 239000008267 milk Substances 0.000 description 4
- 210000004080 milk Anatomy 0.000 description 4
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 4
- 101100328086 Caenorhabditis elegans cla-1 gene Proteins 0.000 description 3
- 229910000831 Steel Inorganic materials 0.000 description 3
- 241000209140 Triticum Species 0.000 description 3
- 235000021307 Triticum Nutrition 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 238000005238 degreasing Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000010959 steel Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000605900 Butyrivibrio fibrisolvens Species 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 240000005979 Hordeum vulgare Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 2
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 229910000389 calcium phosphate Inorganic materials 0.000 description 2
- 235000011010 calcium phosphates Nutrition 0.000 description 2
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- NEFBYIFKOOEVPA-UHFFFAOYSA-K dicalcium phosphate Chemical compound [Ca+2].[Ca+2].[O-]P([O-])([O-])=O NEFBYIFKOOEVPA-UHFFFAOYSA-K 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000006317 isomerization reaction Methods 0.000 description 2
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 2
- 229960004488 linolenic acid Drugs 0.000 description 2
- 235000012054 meals Nutrition 0.000 description 2
- 229930182817 methionine Natural products 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 229910000391 tricalcium phosphate Inorganic materials 0.000 description 2
- 235000019731 tricalcium phosphate Nutrition 0.000 description 2
- 229940078499 tricalcium phosphate Drugs 0.000 description 2
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 2
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 2
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 2
- GKJZMAHZJGSBKD-UHFFFAOYSA-N (10E,12E)-Octadeca-9,11-dienoic acid Natural products CCCCCC=CC=CCCCCCCCCC(O)=O GKJZMAHZJGSBKD-UHFFFAOYSA-N 0.000 description 1
- GKJZMAHZJGSBKD-BLHCBFLLSA-N (10e,12e)-octadeca-10,12-dienoic acid Chemical compound CCCCC\C=C\C=C\CCCCCCCCC(O)=O GKJZMAHZJGSBKD-BLHCBFLLSA-N 0.000 description 1
- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 1
- GKJZMAHZJGSBKD-ANYPYVPJSA-N 10-trans-12-cis-linoleic acid Natural products CCCCCC=C\C=C\CCCCCCCCC(O)=O GKJZMAHZJGSBKD-ANYPYVPJSA-N 0.000 description 1
- OYHQOLUKZRVURQ-UHFFFAOYSA-N 9,12-Octadecadienoic Acid Chemical compound CCCCCC=CCC=CCCCCCCCC(O)=O OYHQOLUKZRVURQ-UHFFFAOYSA-N 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102100034543 Fatty acid desaturase 3 Human genes 0.000 description 1
- 108010087894 Fatty acid desaturases Proteins 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 241000269979 Paralichthys olivaceus Species 0.000 description 1
- 241000282849 Ruminantia Species 0.000 description 1
- 208000005718 Stomach Neoplasms Diseases 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 230000005587 bubbling Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 235000020669 docosahexaenoic acid Nutrition 0.000 description 1
- 150000002066 eicosanoids Chemical class 0.000 description 1
- 235000020673 eicosapentaenoic acid Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000021588 free fatty acids Nutrition 0.000 description 1
- 206010017758 gastric cancer Diseases 0.000 description 1
- 235000021312 gluten Nutrition 0.000 description 1
- 125000005456 glyceride group Chemical group 0.000 description 1
- 230000009422 growth inhibiting effect Effects 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 238000005984 hydrogenation reaction Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
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- 150000002500 ions Chemical class 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000014593 oils and fats Nutrition 0.000 description 1
- 210000004798 organs belonging to the digestive system Anatomy 0.000 description 1
- 238000003359 percent control normalization Methods 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
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- 230000002787 reinforcement Effects 0.000 description 1
- 210000004767 rumen Anatomy 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 201000011549 stomach cancer Diseases 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Birds (AREA)
- Fodder In General (AREA)
Abstract
본 발명은 공역화 리놀레인산(CLA:Conjugated Linoleic acid)을 함유한 사료조성물에 관한 것이다. 종래의 방법으로 제조된 공역화 리놀레인산은 조성물내 지방산화물 함량이 15∼20%로 높으며 cis-9와 trans-11의 CLA 및 trans-10와 cis-12의 CLA 간의 상호 함량 비율을 조절할 수가 없었다. 본 발명은 지방산화물이 제거된 순수한 CLA제품 및 cis-9, trans-11과 trans-10, cis-12 CLA의 상호비율이 조절된 CLA를 단위(單胃)가축의 배합사료에 첨가하여 가축의 성장촉진효과 뿐만 아니라 산란계에 급여하면 계란의 난각질이 강화되고 천연의 트리글리세라이드 형태의 CLA가 함유된 난황유를 얻을 수 있고, 돼지에 급여하면 천연 CLA 함량이 풍부한 고기를 얻을 수 있으며 등지방에서 기름을 추출하면 천연의 트리글리세라이드 형태의 CLA가 함유된 돈지를 얻을 수 있다. 또한 개, 육용오리, 메추리와 같은 단위(單胃)가축용 사료에 CLA를 사료조성물에 사용하면 지방 및 콜레스테롤 함량이 적고 CLA함량이 높은 고기 및 알을 얻을수 있다.The present invention relates to a feed composition containing Conjugated Linoleic acid (CLA). The conjugated linoleic acid prepared by the conventional method has a high fat oxide content of 15 to 20% in the composition and cannot control the ratio of the mutual content between CLA of cis-9 and trans-11 and CLA of trans-10 and cis-12. . The present invention provides a pure CLA product free of fatty oxides and CLA having a controlled mutual ratio of cis-9, trans-11, trans-10, and cis-12 CLA to a unit livestock feed. In addition to the growth promoting effect, feeding the laying hens enhances egg shell quality and provides egg yolk oil containing CLA in the form of natural triglycerides, and feeding pigs gives meat rich in natural CLA content and oil from the back fat. Extraction of lactic acid can produce lard containing CLA in the form of natural triglycerides. In addition, when CLA is used in feed composition for unit livestock such as dogs, ducks and quails, meat and eggs with low fat and cholesterol content and high CLA content can be obtained.
Description
본 발명은 리놀레인산(Linoleic acid) 함량이 풍부한 식용유지 원료를 이성화(isomerization) 반응시켜 얻은 공역화 리놀레인산(Conjugated Linoleic Acid: 이하 CLA라 함)을 함유한 사료조성물에 관한 것이다. 지방산중 리놀레인산 (Linoleic acid: 이하 LA라 함)은 cis-9, cis-12 배열(configuration)에 이중결합을 가지고 있는 탄소수 18개의 지방산이며, CLA란 cis 혹은 trans 배열에 공역화 2중결합(conjugated double bonds)을 가지고 있는 리놀레인산의 위치적 및 형태적 이성체(positional and geometric isomers)를 말한다. 이들 CLA는 9, 11위치 및 10, 12위치에 cis 혹은 trans 배열을 하기도 하며 이론적으로는 리놀레인산(cis-9, cis-12 octadecadienoic acid)의 이성화반응에 의하여 8개의 9, 11 옥타디카디에노인산(octadecadienoic acid) 및 10, 12 옥타디카디에노인산(cis-9, cis-11; cis-9, trans-11; trans-9, cis-11; trans-9, trans-11; cis-10, cis-12; cis-10, trans-12; trans-10, cis-12 및 trans-10, trans-12)이 형성될 수 있다.The present invention relates to a feed composition containing a conjugated linoleic acid (hereinafter referred to as CLA) obtained by isomerization of an edible oil-rich raw material rich in linoleic acid. Linoleic acid (LA) is a fatty acid with 18 carbon atoms with double bonds in cis-9 and cis-12 configurations, and CLA is a conjugated double bond in cis or trans configuration. It refers to the positional and geometric isomers of linoleic acid with conjugated double bonds. These CLAs also have cis or trans arrays at positions 9, 11 and 10, 12. In theory, these CLAs are 8, 9, 11 octadecadies by isomerization of linoleic acid (cis-9, cis-12 octadecadienoic acid). Octadecadienoic acid and 10, 12 octadecadienoic acid (cis-9, cis-11; cis-9, trans-11; trans-9, cis-11; trans-9, trans-11; cis- 10, cis-12; cis-10, trans-12; trans-10, cis-12 and trans-10, trans-12) can be formed.
그러나 자연계에 존재하는 식품중에 CLA가 함유되기도 하며, 특별히 반추동물이 생산하는 우유 및 고기에 자연적으로 함유되어 있는데 총 CLA중 cis-9, trans-11 옥타디카디에노인산이 85∼95%를 차지하고 있다는 보고도 있으나 단위동물이 생산하는 축산물 즉 계란, 닭고기, 돼지고기, 메추리알, 개고기, 오리고기 등에는 CLA함유량이 거의 없는 경우가 많으며 간혹 함유되고 있더라도 우유나 소고기에 함유된 양의 1/10이하의 극소량이 존재할 뿐이다. 이러한 이유는 반추동물의 경우 CLA는 불포화 지방산인 LA가 수소첨가반응이 되는 과정 중의 대사산물로 형성이 되는데 단위동물의 경우는 Rumen 미생물인 부티리비브리오 피브리솔벤 (Butyrivibrio fibrisolvens)에 의하여 이러한 기능을 담당 하는 미생물 및 소화기관이 없기 때문이다.However, some of the foods in nature contain CLA, especially in ruminant-produced milk and meat. Cis-9 and trans-11 octadadienoic acid account for 85-95% of total CLA. Although there are reports, animal products produced by unit animals, such as eggs, chicken, pork, quail eggs, dog meat, and duck meat, have little CLA content, and sometimes even less than 1/10 of the amount of milk or beef. There is only a very small amount of. The reason for this is that in ruminants, CLA is formed as a metabolite during the hydrogenation process of LA, which is an unsaturated fatty acid, but in the case of unit animals, the rumen microorganism, butyrivibrio fibrisolvens (Butyrivibrio fibrisolvens) This is because there is no microorganism and digestive organs in charge.
한편, 미국특허 5,770,247호는 LA 및 리놀레닌산 함량이 높은 유지를 소사료에 1∼5% 첨가하여 천연 CLA함량이 높은 우유를 생산하는 방법으로서 CLA는 항산화 효과, 미생물 성장억제 효과, 위암, 유방암 등에 우수한 효과, 혈액내 콜레스테롤 저하효과, 체지방을 분해효과, 근육내 단백질 함량을 증가 효과 등이 알려져 있다.On the other hand, US Patent No. 5,770,247 is a method of producing milk with a high content of natural CLA by adding 1-5% fat and oil with high content of LA and linolenic acid, CLA has an antioxidant effect, microbial growth inhibitory effect, stomach cancer, breast cancer Excellent effects on the back, blood cholesterol lowering effect, body fat breakdown effect, increase the protein content in the muscle is known.
CLA중 이러한 유익한 효능을 가지고있는 것은 cis-9, trans-11과 trans-10, cis-12 이성체로써 이들중 강력한 생리활성을 나타내는 것은 cis-9, trans-11 CLA이며 trans-10, cis-12 CLA도 구조상의 유사성으로 인하여 Δ-9 디세츄레이스 활성 (desaturase activity)을 억제하여 PGE2생합성을 감소시키므로 에이코노이드 (eicosanoid) 합성의 강력한 저해제 역할을 수행하고 있으므로 cis-9, trans-11 및 trans-10, cis-12의 상호비율이 여러 가지 생리학적인 효능을 발휘하는데 중요한 것으로 알려지고 있다.Among these CLAs, cis-9, trans-11 and trans-10, and cis-12 isomers are cis-9, trans-11 CLA and trans-10, cis-12. CLA also acts as a potent inhibitor of eicosanoid synthesis by inhibiting Δ-9 desaturase activity and reducing PGE 2 biosynthesis due to structural similarity, thus cis-9, trans-11 and trans The mutual ratio of -10 and cis-12 is known to be important for various physiological effects.
한편, CLA에 관련된 종래기술은 미국특허 5,070,104 이외에도 다수 (5,208,356, 5,428,072, 5,430,066, 5,504,114, 5,554,646, 5,585,400, 5,674,901, 5,760,082, 5,760,083, 5,770,247, 5,814,663, 5,827,885, 5,837,733, 5,851,572, 5,856,149)가 있으며, 한국공개특허 98-26382호는 합성한 공역 리놀레인산을 0.5-5%의 농도로 닭사료 또는 넙치용 사료 및 기능성 달걀과 고기를 생산하는 방법이 기재되어 있으나 이들 방법에 의하여 제조된 CLA에는 지방산화물 함량이 높아서 가축에 급여할 경우 성장율이 저하되는 문제점이 있다.On the other hand, the related art related to CLA is in addition to the US patent 5,070,104 (5,208,356, 5,428,072, 5,430,066, 5,504,114, 5,554,646, 5,585,400, 5,674,901, 5,760,082, 5,760,083, 5,770,247, 5,814,663, 5,83,88,5,83,88,5,83,88,5,83,85,85) 98-26382 describes a method for producing synthetic feed conjugated linoleic acid at a concentration of 0.5-5% for chicken feed or olive flounder and functional eggs and meat, but the CLA produced by these methods does not contain fat oxide content. There is a problem in that the growth rate is lowered when fed to livestock.
본 발명은 상기의 문제점인 CLA에 함유된 지방산화물이 제거된 순수한 CLA 제품 및 cis-9, trans-11과 trans-10, cis-12 CLA의 상호비율이 조절된 CLA를 단위(單胃)가축의 배합사료의 조성물로 사용하여 가축의 성장촉진효과, 계란의 난각질 강화효과, 천연의 트리글리세라이드 형태의 CLA가 함유된 난황유를 얻는 효과, 천연의CLA 함량이 높은 돼지고기 및 돼지의 등지방에서 천연의 트리글리세라이드 형태의 CLA가 함유된 돈지를 얻을 수 있다. 또한 개, 육용오리, 메추리와 같은 단위(單胃)가축용 사료에 CLA를 사료조성물에 사용하면 지방 및 콜레스테롤 함량이 적고 CLA 함량이 높은 고기 및 알(egg)을 얻을 수 있는 사료조성물을 제공하는 데 있다.The present invention provides a pure CLA product from which the fatty oxides contained in the CLA is removed, and a CLA in which the mutual ratios of cis-9, trans-11, trans-10, and cis-12 CLA are controlled. It is used as a composition in the feed composition of the animal to promote the growth effect, the egg shell egg reinforcement effect, to obtain egg yolk oil containing CLA in the form of natural triglycerides, in the back fat of pork and pork with high natural CLA content Pigs containing CLA in the form of natural triglycerides can be obtained. In addition, when CLA is used in feed composition for unit livestock such as dogs, ducks and quails, it provides a feed composition that can obtain meat and eggs containing low fat and cholesterol and high CLA content. There is.
도 1은 산란계용 배합사료 조성물을 대조구(-*-), CLA 1%(-+-), 3%(-▲-), 5%(-◆-)로 하여 사료급여 기간에 따른 계란의 생산 그래프이다.1 shows the production of eggs according to the feeding period as a combination feed composition for laying hens control (-*-), CLA 1% (-+-), 3% (-▲-), 5% (-◆-) It is a graph.
본 발명은 리놀레인산이 풍부한 식용유지를 기질로 하여 에틸렌글리콜, 알칼리 이온농도, N2투입량, 반응온도와 시간, pH 조절 및 원심분리에 의하여 수분과 에틸렌글리콜이 제거된 CLA를 얻는다. 또한 질소투입량 및 수소투입량을 변화시켜 생산되는 CLA의 cis-9, trans-11 및 trans-10, cis-12의 상호비율의 조절이 가능하도록 하고, 아울러 생리적인 기능성이 없는 cis-9, cis-11; cis-10, cis-12 및 trans-9, trans-11 옥타디카디에노인산의 생성량을 최저화한다. 이와 같이 생산된 CLA는 지방의 고온반응으로 인하여 생성된 지방산화물이 혼탁한 황색 내지는 갈색을 띄게 되므로 불순물을 제거하여 지방산화물이 100% 제거된 무색투명한 순품의 CLA를 단위(單胃)가축의 사료조성물로 사용한다.The present invention obtains CLA from which water and ethylene glycol are removed by ethylene glycol, alkali ion concentration, N 2 input, reaction temperature and time, pH control and centrifugation using edible oil-rich edible oil as a substrate. In addition, it is possible to control the mutual ratio of cis-9, trans-11, trans-10, and cis-12 of CLA produced by varying the amount of nitrogen and hydrogen, and cis-9, cis- without physiological function. 11; Minimize the amount of cis-10, cis-12 and trans-9, trans-11 octadicadienoic acid. The CLA produced in this way has a cloudy yellow or brown color of the fat oxide produced by the high temperature reaction of fat, so that the impurities are removed and 100% of the colorless transparent pure CLA is removed. Used as a composition.
다음의 실시예 및 시험예는 본 발명을 보다 상세히 설명하고자 하는 것으로 이들이 본 발명의 기술적 범위를 한정하는 것은 아니다.The following examples and test examples are intended to explain the present invention in more detail, but do not limit the technical scope of the present invention.
< 실시예 1 > 식용유지에서 순품 CLA의 제조Example 1 Preparation of Pure CLA in Edible Oils and Fats
에틸렌글리콜을 150g∼75g 및 NaOH 3g∼25g을 원형 3구 플라스크에 넣고 N2를 공급(bubbling)하여 140℃ 온도로 용해시킨 후, 홍화유는 15g∼75g의 범위로 첨가하여 140℃∼200℃의 온도범위에서 30분∼3시간별로 반응시킨다. 반응이 종료되면 6N-HCl용액을 사용하여 pH 4.5∼pH 5.0으로 조절한다. 그후 반응물을 15∼30분 정치시켜 상층부를 분리하여 유리지방산 상태의 CLA를 얻었다.150 g to 75 g of ethylene glycol and 3 g to 25 g of NaOH were placed in a round three-necked flask and bubbling N 2 to dissolve it at 140 ° C. Then, safflower oil was added in the range of 15 g to 75 g and 140 ° C. to 200 ° C. The reaction is carried out in the temperature range for 30 minutes to 3 hours. After the reaction was completed, the pH was adjusted to 4.5 to pH 5.0 using 6N-HCl solution. Thereafter, the reaction was allowed to stand for 15 to 30 minutes to separate the upper layer, thereby obtaining CLA having a free fatty acid state.
이들 CLA에 함유된 각각의 지방산 조성 및 생산된 CLA중량당 산화물 함량을 분석을 통한 최적의 반응조건으로 가성소다 사용량에 따른 에틸렌글리콜 150g, 홍화유 15g, 온도 180℃, 2시간 반응시, NaOH 3.1g의 조건에서 산화물 1.2%로 나타났다. 홍화유 사용량에 따른 에틸렌글리콜 150g, NaOH 11.3g, 온도 180℃, 2시간 반응시, 홍화유 15g 조건에서 산화물 2.5%로 나타났다. 반응온도에 따른 에틸렌글리콜 150g, 홍화유 40g, NaOH 11.3g에서 140℃의 조건에서 산화물0.5%로 나타났다. 반응시간에 따른 CLA와 산화물 생성율은 에틸렌글리콜 150g, 홍화유 40g, NaOH 11.3g, 온도 180℃, 0.5시간 반응조건에서 산화물은 검출되지 않았다.According to the optimum reaction conditions through analyzing the fatty acid composition and the amount of oxides per weight of CLA produced in these CLAs, 150 g of ethylene glycol according to the amount of caustic soda, 15 g safflower oil, 180 ° C. for 2 hours, NaOH 3.1 g The oxide was found to be 1.2%. Ethylene glycol 150g according to the amount of safflower oil, 11.3g NaOH, temperature 180 ℃, reaction for 2 hours, safflower oil was found to be an oxide 2.5% under 15g conditions. Ethylene glycol 150g, safflower oil 40g, NaOH 11.3g according to the reaction temperature was found to be 0.5% oxide at 140 ℃. According to the reaction time, the production rate of CLA and oxide was not detected in the reaction conditions of ethylene glycol 150g, safflower oil 40g, NaOH 11.3g, temperature 180 ℃, 0.5 hours.
에틸렌글리콜 첨가량에 따른 CLA와 산화물 생성율은 홍화유 40g, NaOH 11.3g, 온도 180℃, 2시간 반응의 조건에서 산화물 5.6g으로 나타났다.The production rate of CLA and oxide according to the amount of ethylene glycol added was 5.6 g of oxide under the conditions of safflower oil 40 g, NaOH 11.3 g, temperature 180 ° C., and reaction for 2 hours.
또한 압력반응에 의한 LA에서 CLA중 cis-9, trans-11와 trans-10, cis-12 CLA의 상호비율이 조절된 CLA의 생산시 LA(함량 60%) 100g, 에틸렌글리콜 350g, 및 NaOH 10g∼30g을 반응기에 넣고, N2gas는 1,000㎖∼500㎖/min, H2gas는 30㎖∼15㎖/min으로 흘리면서 180℃에서 1시간 반응시킨 후 6N-HCl용액으로 pH 4.5∼5.0으로 조절한 후 원심분리하여 CLA를 회수한다. NaOH 첨가량에 따른 cis-9, trans-11와 trans-10, cis-12 CLA의 상호비율은 NaOH 10g에서 1:8.7, 15g에서 1:1.13, 20g에서 1:1.08, 25g에서 1:0.43, 30g에서 1:0.18로 나타났다.In addition, LA (content 60%) 100 g, ethylene glycol 350 g, and NaOH 10 g in the production of CLA in which the mutual ratio of cis-9, trans-11 and trans-10, cis-12 CLA in the CLA in the LA by pressure reaction was controlled ~ 30g was put into the reactor, N 2 gas was flowed at 1,000ml ~ 500ml / min, H 2 gas was flowed at 30ml-15ml / min for 1 hour at 180 ° C, and then the pH was 4.5-5.0 with 6N-HCl solution. After adjustment, CLA is recovered by centrifugation. The ratio of cis-9, trans-11, trans-10, and cis-12 CLA according to the amount of NaOH added was 1: 8.7 in NaOH 10: 1, 1: 1.13 in 15g, 1: 1.08 in 20g, 1: 0.43, 30g in 25g Found in 1: 0.18.
이와 같이 생성된 황색 내지 갈색의 지방 산화물질을 제거하기 위하여 발명자가 제작한 초임계 이산화탄소 추출장치를 이용하여 산화물을 제거하였다.Oxides were removed using a supercritical carbon dioxide extractor manufactured by the inventors to remove the yellow to brown fatty oxides thus produced.
< 실시예 2 > CLA 함유여부에 따른 단위가축용 사료 조성물<Example 2> feed composition for unit livestock according to the content of CLA
단위가축용 사료조성물의 범위는 곡류 사료원을 45∼70%, 강피류 사료원을 5∼15%, 유지 사료원 0.3%이상, 식물성 단백질 사료원 5∼15%, 동물성 단백질 사료원 2∼10% 및 광물질 사료원, 비타민제, 아미노산공급제, 항산화제, 효소 및 생균제, 성장 촉진제 등을 3∼6%의 범위로 하여 전체 중량비를 100%로 하였다. 즉 본 발명에 의한 CLA 함유 사료조성물에서는 상기에서 생산된 CLA 조성물을 유지사료원으로 하여 배합사료에 중량비로 0.3%이상 혼합하고, 이때 전체 배합사료 조성물의 총 지방함량이 3.0%이상이 되도록 사료원의 배합사료비율을 조절한다. 이때 가축의 사양목적에 따라서 생산된 CLA 조성물을 배합사료에 첨가하는 함량을 달리하게 되는바, 예로써 CLA 조성물을 0.3% 이상 배합하였을 때 전체 배합사료에 포함된 총 지방산 조성을 조절하여 표 1에 제시된 CLA 함량 및 비율을 유지해야만 목적하는 효과를 얻을 수 있다.The range of unit livestock feed composition ranges from 45 to 70% for grain feed sources, 5 to 15% for barley feed sources, 0.3% for oil and fat feed sources, 5 to 15% for vegetable protein feed sources, and 2 to 10 for animal protein feed sources. % And mineral feed source, vitamins, amino acid feed, antioxidants, enzymes and probiotics, growth promoters, etc. in the range of 3 to 6%, the total weight ratio was 100%. In other words, in the CLA-containing feed composition according to the present invention, the above-mentioned CLA composition is mixed with the blended feed in a weight ratio of 0.3% or more, wherein the total fat content of the total blended feed composition is 3.0% or more. Adjust the blended feed rate. At this time, the content of adding the produced CLA composition to the blended feed is changed according to the specification purpose of the livestock. For example, when 0.3% or more of the CLA composition is blended, the total fatty acid composition included in the total blended feed is adjusted to show the results. Maintaining the CLA content and ratio will achieve the desired effect.
따라서 배합사료원료 중 곡류 등에는 2∼5%의 지방이 함유되고 있으므로 실제 사용량을 조절하여 총지방산의 조성 중 cis-9, trans-11 CLA 는 적어도 1.5%이상이고, cis-9, trans-11 및 trans-10, cis-12 CLA는 3.0% 이상이 되도록 사료조성물을 조절하여야 한다. 이것은 사용하는 사료원 및 유지에 포함된 지방함량 및 서로 상이한 지방산 조성에 의하여 CLA 조성물을 첨가할 때 최종배합사료 조성물에서 희석되는 효과가 발생하므로 CLA조성물 사용효과를 얻기 위해서는 반드시 총 지방함량이 3% 이상이며, 총 지방산 조성 중 cis-9, trans-11 CLA 함량이 1.5% 이상이 되도록 사료조성물을 만들어야 한다.Therefore, grains, etc., contain 2 to 5% of fat in the blended feedstock, so the amount of cis-9 and trans-11 CLA in the composition of total fatty acids is at least 1.5%, cis-9, trans-11 And trans-10, cis-12 CLA should be adjusted to more than 3.0% feed composition. This results in the dilution effect in the final blended feed composition when the CLA composition is added due to the fat content and different fatty acid composition included in the feed source and fat used, so that the total fat content must be 3% to obtain the effect of using the CLA composition. Above, the feed composition should be made so that the content of cis-9 and trans-11 CLA in the total fatty acid composition is 1.5% or more.
표 1. 배합사료조성물에서 CLA함량 및 비율Table 1.CLA Contents and Ratios in Blended Feed Compositions
상기와 같이 하여 얻은 CLA조성물을 이용하여 동물의 성장촉진, CLA가 함유된 축산물의 생산 및 천연 CLA 함유된 동물성 유지를 생산하고자 실제로 어린 육계, 어린돼지, 산란계 및 비육돈에 급여하여 효과를 평가하였다.The CLA composition thus obtained was used to evaluate the effects of actually feeding young broilers, young pigs, laying hens and finishing pigs to promote animal growth, produce livestock products containing CLA, and produce animal fats containing natural CLA.
< 실시예 3 > 어린육계에서 성장효과Example 3 Growth Effect in Young Broilers
본 발명에서 제조된 지방산화물이 함유된 제품과 지방산화물이 100% 제거된 CLA제품을 통상의 어린육계용 배합사료에 중량비율로 0.3% 및 1.0%되는 량을 첨가하여 처리구당 15마리씩 배치하여 3주령까지 사육하였다. 표 2에 사용한 배합사료의 조성비를 제시하였다.The fat-containing product prepared in the present invention and the CLA product 100% of the fat oxide is removed by adding the amount of 0.3% and 1.0% by weight ratio to the conventional broiler feed compound and placed 15 per treatment 3 Breeding by age. The composition ratio of the blended feed used in Table 2 is shown.
표 2. 어린 육계용 배합사료 조성물Table 2. Compound Feed Composition for Young Broilers
※상기에서 CLA는 지방산화물이 제거된 것과 함유된 것의 배합비율은 동일함.※ In the above, CLA has the same compounding ratio as those containing fatty oxides removed.
표 3. CLA의 지방산화물 함유유무에 따른 육계의 성장효과Table 3. Growth Effect of Broiler with and without CLA
즉, 사육기간이 경과함에 따라서 모든 CLA 첨가구에서는 대조구에 비하여 성장이 촉진되었으나, 산화물을 제거한 CLA 급여구에서 성장율이 가장 향상되었으며, 대조구와 대비하여 산화물 제거한 CLA 1% 처리구에서는 성장율이 20% 향상되었으나 산화물 제거 전 CLA 0.3% 처리구에서는 성장율이 5.9% 향상되어 큰 차이점이 있다는 것을 알 수 있다.That is, as the breeding period elapsed, the growth was accelerated in all the CLA-added diets compared to the control, but the growth rate was the highest in the CLA-treated diets without oxides, and the growth rate was increased by 20% in the 1% CLA-treated diets compared to the control. However, it can be seen that there is a big difference in the growth rate of 5.9% in the CLA 0.3% treatment before oxide removal.
< 실시예 4 > 어린 돼지에서 성장효과Example 4 Growth Effect in Young Pigs
실시예 2와 동일하게 CLA를 어린 돼지 배합사료에 각각 0.5%, 1.0% 첨가하여 체중 5㎏의 어린돼지를 각 처리구 당 5마리의 돼지를 4주간 사육하여 체중을 비교하였다.In the same manner as in Example 2, CLA was added 0.5% and 1.0% to the young pig compound feed, respectively, and 5 kg of young pigs weighing 5 pigs for each treatment were bred for 4 weeks to compare their weight.
표 4. 어린 돼지용 배합사료 조성물Table 4. Compounded feed composition for young pigs
※ CLA기름은 산화물제거전 및 제거후로 나누며 배합비율은 동일함.※ CLA oil is divided into before and after oxide removal, and the mixing ratio is the same.
표 5. CLA의 지방산화물 함유유무에 따른 돼지의 성장효과Table 5. Growth effect of pigs with and without CLA
어린 돼지의 성장률 또한 산화물 제거전 CLA 급여구 중 1.0% 급여구의 경우 2주일 경과시부터 대조구에 비하여 성장속도가 향상되었으나, 다른 CLA 급여구와 비교시 정체되는 경향을 나타내었다. 그러나 산화물 제거한 CLA 0.5% 및 1.0% 급여구에서는 계속하여 우수한 성장율을 나타냈다.The growth rate of young pigs was also higher than that of the control group after 2 weeks in the 1.0% CLA diet before the oxide removal, but showed a stagnant trend compared to the other CLA diets. However, the 0.5% and 1.0% CLA-free diets continued to show excellent growth rates.
< 실시예 5 > 산란계의 계란생산율 및 난각품질에 미치는 영향Example 5 Effects on Egg Production Rate and Eggshell Quality of Laying Hens
일반적으로 리놀산, 리놀렌산, EPA 및 DHA와 같은 지방산 함량이 많은 계란을 만들기 위하여 산란계에 대하여 이들 성분이 풍부한 지방 및 지방산을 섭취시키면 목적하는 유효성분 함량은 증가하는 반면에 계란의 생산율이 저하되고, 또한 계란의 난각두께가 얇아져 깨지는 비율이 증가하여 난각품질이 저하하는 문제점이 있다. 본 발명에서 생산된 CLA제품을 산란계에 장기간 급여할 때의 효과를 파악하기 위하여 실시예 2와 같이 CLA의 지방산화물이 함유된 CLA 급여구 및 제거된 CLA제품을 통상의 산란계용 배합사료에 2.5% 및 5.0%되는 양으로 배합하여 처리구당 25마리의 산란계를 배치하여 3개월간 실험하였다.In general, ingesting these ingredients-rich fats and fatty acids for laying hens to make eggs rich in fatty acids, such as linoleic acid, linolenic acid, EPA and DHA, increases the desired active ingredient content while lowering egg production. Egg shell thickness is thin, the rate of cracking is increased, there is a problem that the egg shell quality is reduced. In order to grasp the effect of long-term feeding of the CLA product produced in the present invention to laying hens, 2.5% of the CLA feeder containing CLA fatty oxides and the removed CLA product in a conventional diet for laying hens as in Example 2 And 25 laying hens per treatment group in the amount of 5.0% and tested for 3 months.
표 6. 산란계용 배합사료 조성물Table 6. Combined feed composition for laying hens
※ CLA기름은 산화물제거전 및 제거후로 나누며 배합비율은 동일함.※ CLA oil is divided into before and after oxide removal, and the mixing ratio is the same.
상기 표 6의 배합사료 조성물은 대조구의 총 지방함량은 3.5%이고 처리구에서는 각각 6.0% 및 8.5%로 하였다. 이것은 산란계에서 배합사료내 지방함량이 증가하면 일반적으로 난각 품질이 극도로 저하되는 문제점을 해결할 수 있는지 여부를 평가하기 위한 것이다.In the blended feed composition of Table 6, the total fat content of the control was 3.5% and treated with 6.0% and 8.5%, respectively. This is to evaluate whether it is possible to solve the problem that the egg quality is generally reduced when the fat content in the diet is increased in laying hens.
표 7. CLA의 지방산화물 함유유무에 따른 계란의 품질효과Table 7. Quality Effects of Eggs with and without CLA
상기 표 7에서 나타난 바와 같이 CLA의 지방산화물 함유여부에 따라 많은 차이점이 발견되었는데 산화물 제거 전 CLA 급여구의 여러항목의 성적과 대조구간에는 서로 비숫한 성적을 나타냈으나 산화물 제거한 CLA 급여시는 모든 조사항목에서 크게 향상된 효과를 나타냈다. 즉 CLA 5.0% 급여시에도 산란율이 12%나 향상되었고 파열강도도 대조구는 3.08㎏, CLA 5.0% 급여구는 3.45㎏으로 크게 강화되었고 따라서 난각두께도 0.395㎜로써 가장 우수하였다.As shown in Table 7, many differences were found depending on whether CLA contained fatty oxides. The results of various items in the CLA supplemented group before the removal of oxides were similar to those in the control group. Showed a significantly improved effect. In case of CLA 5.0%, egg production was improved by 12%, and the rupture strength control was 3.08㎏ and CLA 5.0% was 3.45㎏, and egg shell thickness was 0.395mm.
< 실시예 6 > 고농도의 CLA 함유 계란과 천연 CLA 함유의 난황유 생산Example 6 Production of High Concentration Eggs Containing CLA and Egg Yolk Containing Natural CLA
고농도의 CLA가 함유된 계란의 생산과 cis-9, trans-11 CLA 및 trans-10, cis-12 CLA의 비율이 조절된 천연 CLA 함유 난황유의 생산을 위하여 본 발명의 산화물이 제거된 CLA를 표 8과 같은 산란계용 배합사료에 첨가하여 급여시켰다.Table 1 shows the oxide-free CLA of the present invention for production of eggs containing high concentrations of CLA and production of natural CLA-containing egg yolk oil with controlled ratios of cis-9, trans-11 CLA and trans-10, cis-12 CLA. It was added to the diet for laying hens such as 8 and fed.
표 8. 산란계용 배합사료 조성물Table 8. Combined feed composition for laying hens
산란일령 30주령의 평균산란율 85%인 산란계를 처리당 50수씩 배치하였고 대조구 및 처리구로 CLA 1%, 3%, 5%를 첨가하였다. 도 1과 같이 CLA 사료 급여 시 사양기간 경과에 따라 일주일만에 CLA함량이 풍부한 계란을 생산할 수 있었다. 특히 본 실시예에서 생산된 CLA의 조성이 cis-9, trans-11 및 trans-10, cis-12 CLA의 상호비율이 1:1.02인 CLA제품을 급여하였으나 계란에 축적되는 CLA 조성에서는 cis-9, trans-11 및 trans-10, cis-12 CLA의 상호비율이 1:0.5인 천연 트리글리세라이드 형의 CLA를 함유한 난황유의 생산이 가능하였다. 이렇게 생산된 계란에는 cis-9, trans-11 및 trans-10, cis-12 CLA함량이 계란 100g당 600∼675㎎함유되어 있으며, 또한 생산된 난황은 통상의 방법으로 기름을 추출하였을 때 천연상태의 트리글리세라이드 형의 CLA가 함유된 난황유를 생산하였다.Laying hens with an average egg yield of 85% at 30-weeks of age, 50 eggs per treatment were placed, and 1%, 3%, and 5% CLA was added to the control and treatment. As shown in FIG. 1, the CLA feed was able to produce eggs rich in CLA in one week according to the specification period. In particular, the CLA composition produced in this Example was cis-9, trans-11 and trans-10, cis-12 CLA product of the CLA product ratio of 1: 1.02, but the CLA composition accumulated in eggs cis-9 It was possible to produce egg yolk oil containing CLA of the natural triglyceride type having a ratio of 1: 0.5, trans-11, trans-10, and cis-12 CLA. Eggs produced in this way contain cis-9, trans-11 and trans-10, cis-12 CLA content of 600 ~ 675mg per 100g of egg, and the produced egg yolk is natural when oil is extracted by conventional methods. The yolk oil containing CLA of the triglyceride type of was produced.
< 실시예 7 > 고농도의 CLA 함유 돈육과 돈지 생산Example 7 Production of High Concentration CLA-containing Pork and Pork
평균체중 25㎏의 숫퇘지를 대상으로 생산된 CLA 제품 중 cis-9, trans-11 및 trans-10, cis-12 CLA의 비율이 1:0.5인 제품을 2.5%, 5.0%를 표 9와 같은 통상의 돼지 배합사료에 첨가하여 90㎏이 될 때까지 사육한 후 도살하여 돈육을 분석하였다.2.5% and 5.0% of the CLA products produced with the average weight of 25 kg males with cis-9, trans-11 and trans-10 and cis-12 CLA ratios were 0.5% and 5.0%, respectively. Pig was added to the feed compound of breeding until 90kg and then slaughtered and analyzed pork.
표 9. 육성돼지용 배합사료 조성물Table 9. Combination feed composition for breeding pigs
표 10. 돈육의 CLA 함유량 비교Table 10. Comparison of CLA content in pork
상기의 표 10과 같이 CLA 급여시는 고기에 축적되는 지방산중 C16:1, C18:1 지방산은 크게 감소하였고 C16:0, C18:0 지방산은 반대로 증가하였다. 특히 사료에 첨가한 CLA는 cis-9, trans-11 및 trans-10, cis-12 CLA가 1:0.5인 제품을 급여하였으나 고기내에는 cis-9, trans-11 CLA가 거의 95%이상인 돈육을 생산하였다. 또한 돈육생산 시 부산물로 얻어지는 등지방을 이용하여 통상의 방법으로 돈지를 만들었을때는 항암등의 기능성이 있다고 평가되는 cis-9, trans-11 및 trans-10, cis-12 CLA 중에서 더욱 강한 생리작용을 가지고 있는 cis-9, trans-11 CLA 기름을 천연 글리세라이드 형태로 생산하였다. 또한 콜레스테롤 함량도 14∼20%가 감소하였다.As shown in Table 10, the C16: 1 and C18: 1 fatty acids were significantly decreased and C16: 0 and C18: 0 fatty acids were significantly increased in fatty acids accumulated in meat. In particular, CLA added to the feed was cis-9, trans-11 and trans-10, cis-12 CLA 1: 0.5 products, but meat cis-9, trans-11 CLA is almost 95% more than pork Produced. In addition, when the pork fat is made by the conventional method using the back fat obtained as a by-product of pork production, it has a stronger physiological effect among cis-9, trans-11, trans-10, and cis-12 CLA, which are evaluated as anti-cancer. Cis-9 and trans-11 CLA oils were produced in the form of natural glycerides. Cholesterol content was also reduced by 14-20%.
< 실시예 8 > 토종개 사육시 고기내 지방산 조성의 변화Example 8 Changes in Fatty Acid Composition in Meat during Breeding of Native Dogs
평균체중 10㎏의 토종개를 대상으로 생산된 CLA제품 중 cis-9, trans-11 과 trans-10, cis-12 CLA의 비율이 1:0.2인 제품을 표 11의 토종개 사료의 조성물로 5.0%, 10.0% 첨가하여 150일간 사육하였으며 처리당 각각 4마리의 토종개를 배치하였다.Among the CLA products produced for native dogs with an average body weight of 10 kg, the ratio of cis-9, trans-11, trans-10, and cis-12 CLA in the ratio of 1: 0.2 was 5.0% according to the composition of the native dog food in Table 11, 10.0% was added and raised for 150 days, and 4 native dogs were placed per treatment.
표 11. 토종개용 배합사료 조성물Table 11. Formulated feed composition for native dogs
상기의 표 11과 같은 토종개용 배합사료 조성물을 150일간 사육한 후 도살하여 뒷다리 부분의 시료를 채취하여 지방산 분석을 실시하였다. 결과 표 12에 나타난 바와 같이 일반적인 지방산의 증감 변화(C16:0, C16:1, C18:0, C18:2)는 실시예 7의 돼지 사육 결과와 비슷한 경향을 나타내었다. 그리고 cis-9, trans-11 CLA의 함량은 14%까지 증가되었으나 개사료에 함유된 CLA 함량에서는 35%임을 감안하면 사료내 CLA 첨가량을 높일수록 체내에 축적되는 효율면에서는 크게 개선되지 않는 결과를 보였다.After breeding the mixed feed composition for indigenous dogs as shown in Table 11 for 150 days, slaughtered, samples of the hind legs were collected, and fatty acid analysis was performed. Results As shown in Table 12, the change in general fatty acids (C16: 0, C16: 1, C18: 0, C18: 2) showed similar tendency to the pig breeding results of Example 7. In addition, cis-9 and trans-11 CLA contents were increased up to 14%, but considering that the CLA content of dog food was 35%, the higher the amount of CLA added in the feed, the less the improvement in efficiency accumulated in the body. Seemed.
표 12. 토종개의 고기에서 지방산 조성의 변화Table 12. Changes in fatty acid composition in meat of native dogs
< 실시예 9 > 육용오리용 배합사료에 CLA첨가 효과<Example 9> Addition of CLA to the blended feed for meat duck
육용오리의 배합사료(표 13)에 CLA제품을 2.5% 및 5.0% 첨가하였을 때 오리고기에 함유되는 지방산 및 콜레스테롤 함량을 비교하였다. 오리고기는 불포화 지방산이 높아서 최근에 많이 소비되는 육류로서 개시체중 500g의 숫오리를 처리구별로 15수씩 배치하여 평균체중 3.0㎏까지 사육하였다.When 2.5% and 5.0% of CLA products were added to the blended feed of ducks (Table 13), fatty acid and cholesterol contents of duck meat were compared. Duck meat is a meat that is consumed a lot recently because of high unsaturated fatty acids, and 500g of male ducks were placed at 15 kg of each treatment and raised to an average weight of 3.0kg.
표 1. 육용오리용 배합사료의 조성물Table 1. Composition of Compound Feed for Duck
표 14에 나타난 바와 같이 지방산의 변화하는 경향은 실시예 7의 돼지 및 실시예 8의 개의 경우와 비슷한 결과를 나타냈고 있는 데 이것은 위장이 하나 뿐인 단위 가축에 CLA를 첨가시 모두 비슷한 양상임을 알 수 있다.As shown in Table 14, the changing tendency of fatty acids showed similar results to the pigs of Example 7 and the dogs of Example 8, which is similar to the case of adding CLA to a single livestock with only one stomach. have.
표 14. 오리고기의 지방산 조성(%) 및 콜레스테롤 함량Table 14. Fatty Acid Composition and Cholesterol Content of Duck Meat
특히 오리 사육시 고기내 콜레스테롤 함량이 대조구에 비하여 각각 11.9% 및 17.9%로 감소되었고, cis-9, trans-11 CLA 함량은 9.7% 이었다. 또한 오리고기는 일반적으로 지방함량이 높은데도 불구하고 실험후 도살시 지방함량은 상당히 감소된 것을 알 수 있었다.In particular, the cholesterol content of meat in duck was reduced to 11.9% and 17.9%, respectively, compared to the control, and cis-9 and trans-11 CLA contents were 9.7%. In addition, although the duck meat is generally high in fat, the fat content in the slaughter after the experiment was significantly reduced.
< 실시예 10 > 산란메추리용 사료에 CLA의 첨가 효과<Example 10> Addition effect of CLA to the feed for laying quail
메추리 사료에는 실시예 6의 산란계용 배합사료와 같이 표 15의 산란 매추리용 배합사료에 CLA를 2.0%, 4.0% 배합하였다.In quail feed, 2.0% and 4.0% of CLA was added to the laying feed for laying quail in Table 15, similar to the mixing feed for laying hens in Example 6.
표 15. 산란 메추리용 배합사료 조성비Table 15. Compositional composition of laying feed
표 15와 같은 배합사료를 처리당 25수의 메추리를 배치하여 3주 동안 실험하였고, 3일 간격으로 알을 수집하여 난황에 함유된 지방과 콜레스테롤 함량의 변화를 분석하였다. 표 16에 그 결과를 나타냈으며 산란계의 경우에는 지방산의 변화가 사료 급여후 6일째부터 거의 최고점의 CLA 함량을 유지하였으나, 표 16의 결과에서 보듯이 지방 및 콜레스테롤 함량의 감소효과는 적어도 15일정도 사료를 급여후 나타났다. 지방함량은 21일째에 대조구 대비 각각 33% 및 45% 감소효과를 나타냈고, 콜레스테롤 함량은 각각 30% 및 34% 감소되었다.The mixed feed as shown in Table 15 was experimented for three weeks by placing 25 quails per treatment, and the eggs were collected every three days to analyze the changes in fat and cholesterol content in egg yolk. The results are shown in Table 16. In the case of laying hens, the change of fatty acids maintained almost the highest CLA content from 6 days after feeding, but as shown in the results of Table 16, the effect of reducing fat and cholesterol was at least 15 days. It appeared after feeding. At 21 days, fat content decreased by 33% and 45%, respectively, and cholesterol content decreased by 30% and 34%, respectively.
표 16. 메추리알의 지방함량 및 콜레스테롤 함량 변화Table 16. Changes in fat and cholesterol content of quail eggs
< 시험예 1 ><Test Example 1>
다음은 종래의 방법과 본 발명에 의하여 제조된 산화물 제거 공정을 거치기 전의 생산품을 분석한 결과를 표 17에 나타냈다.Next, Table 17 shows the results of analyzing the products prior to the oxide removal process prepared by the conventional method and the present invention.
표 17. 본 발명과 종래 방법의 CLA 함량 비교Table 17. Comparison of CLA Contents of the Invention and Conventional Methods
표 17과 같이 생리학적 기능성이 없는 CLA, 즉 cis-9, cis-11; cis-10, cis-12 및 trans-9, trans-11의 생성율이 종래의 방법에서는 5.9%∼9.9% 이었으나 본원의 방법에서는 2.25%∼2.9%의 범위로 62%∼71% 정도 줄일 수 있으며 목적으로 생산하고자 하는 cis-9, trans-11과 trans-10, cis-12의 상호 생성비율의 조절 및 함량도 증가시키는 효과가 있다.CLA without physiological functionality, ie cis-9, cis-11; Although the production rate of cis-10, cis-12, trans-9, and trans-11 was 5.9% to 9.9% in the conventional method, the method of the present invention can reduce 62% to 71% in the range of 2.25% to 2.9%. It is also effective to increase the control and content of the mutual generation rate of cis-9, trans-11 and trans-10, cis-12 to be produced.
또한 이렇게 생산된 CLA 혼합물을 기질로 하여 산화물 제거 공정을 거치면 산화물을 100% 제거할 수 있고 또한 생물학적 기능성이 없는 CLA도 95%이상 제거되는 효과가 있으며 무색의 투명한 CLA를 생산할 수 있다.In addition, by using the CLA mixture produced as a substrate, an oxide removal process can remove 100% of oxides, and more than 95% of CLAs without biological function can be removed, and colorless transparent CLA can be produced.
< 시험예 2 ><Test Example 2>
본 발명에서 제조한 CLA 조성물을 가축에게 0.5∼5.0% 수준으로 배합사료에 첨가하여 급여하였을 때 목적하는 효과를 충분히 얻을 수 있다. 즉 외부 환경에 대한 저항력이 약한 어린 가축에서는 우수한 성장 촉진 효과가 나타났으며, 산화물 제거 전의 CLA 조성물을 급여하였을 때도 일반 배합사료 급여한 성적보다도 향상되었다. 또한 계란을 생산할 경우에는 계란의 생산성이 향상되었고 아울러 생산되는 계란 껍질의 품질도 크게 향상시켰으며 천연 상태의 CLA 함유량이 600㎎∼ 675㎎/100g 함유된 계란을 생산하였다. 돼지고기에서는 CLA중 생리활성이 강한 cis-9, trans-11 CLA가 10%함유된 돈육을 생산할 수 있다. 따라서 이러한 CLA 조성물은 단위 가축에게 급여시는 목적하는 효과가 뚜렷하게 나타나는데, 토종개, 육용오리, 메추리 사료에 적용시도 지방 및 콜레스테롤 함량이 아주 낮고 기능성 CLA가 풍부한 고기 및 알을 생산할 수 있다.When the CLA composition prepared in the present invention is added to a feed compound at a level of 0.5 to 5.0% to livestock, the desired effect can be sufficiently obtained. In other words, young livestock with low resistance to the external environment showed excellent growth promoting effects, and even when fed the CLA composition before the removal of oxide, it was improved than the results of the general feed fed. In addition, when eggs were produced, the productivity of eggs was improved, and the quality of egg shells produced was greatly improved. Also, eggs containing 600 mg to 675 mg / 100 g of natural CLA were produced. Pork can produce pork containing 10% of CLA, cis-9 and trans-11 CLA. Therefore, the CLA composition has a distinctive effect when feeding to livestock, and can be used to produce meat and eggs rich in functional CLA with very low fat and cholesterol content when applied to native dogs, ducks and quails.
본 발명의 공역화 리놀레인산은 지방산화물이 100% 제거되었고 생리활성이 없는 CLA는 95%이상 제거된 무색 투명한 CLA로서 최종제품의 CLA중 cis-9, trans-11 CLA 및 trans-10, cis-11 CLA의 상호 함량비율이 조절되어 있다. 따라서 단위동물의 배합사료에 급여하면 어린가축의 성장을 촉진, 계란 100g당 천연상태로 난황에 재합성된 CLA 함유량이 600㎎∼675㎎ 함유된 계란의 생산과 계란의 난각두께 향상, 돼지고기에 CLA함량이 10% 함유된 돈육의 생산 그리고 계란 및 돼지의 등지방에서 천연상태의 트리글리세라이드 형태로 재 합성된 CLA 함량이 풍부한 난황유와 돈지를 얻을 수 있다. 그밖에도 단위가축인 토종개, 육용오리, 산란 메추리에 급여시 지방과 콜레스테롤 함량이 15∼45% 감소되고 기능성 CLA함량이 높은 축산물을 얻을 수 있다.The conjugated linoleic acid of the present invention is a colorless transparent CLA which is 100% free of fat oxide and 95% or more of physiologically active CLA is cis-9, trans-11 CLA and trans-10, cis- in CLA of the final product. The mutual content ratio of 11 CLA is controlled. Therefore, feeding the feed of the unit animal promotes the growth of young livestock, production of eggs containing 600 mg to 675 mg of CLA content reconstituted in egg yolk per 100 g of egg, and egg shell thickness improvement, The production of pork containing 10% CLA content and egg yolk oil and pork fat rich in CLA, re-synthesized in the form of natural triglycerides from the back fat of eggs and pigs. In addition, livestock products, chicken ducks and laying quails, which are unit livestock, reduce fat and cholesterol content by 15-45% and produce livestock products with high functional CLA content.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20010027513A (en) * | 1999-09-14 | 2001-04-06 | 신성수 | manufacture method of fodder |
| KR100308387B1 (en) * | 1999-07-06 | 2001-09-13 | 박구부 | Method for lessening contents of nitrate or nitrite in meat products |
| KR100384368B1 (en) * | 2001-01-31 | 2003-05-22 | 대한제당 주식회사 | Method for Preparing Conjugated Linoleic Acid Composition |
| KR100431906B1 (en) * | 2001-10-16 | 2004-05-17 | 주식회사 애그리브랜드 퓨리나코리아 | Feed increasing CLA content in the milk and manufacturing method thereof |
| KR100889655B1 (en) * | 2006-11-15 | 2009-03-19 | 고려대학교 산학협력단 | Animal Feed Composition containing by-products obtained from purification process of the conjugated linoleic acid |
| WO2016195369A1 (en) * | 2015-06-05 | 2016-12-08 | 윤관식 | Oil and fat composition for feed, and feed additive for growth promotion, livestock feed composition, and livestock breeding method using same |
-
1999
- 1999-04-03 KR KR1019990011775A patent/KR19990073078A/en not_active Application Discontinuation
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100308387B1 (en) * | 1999-07-06 | 2001-09-13 | 박구부 | Method for lessening contents of nitrate or nitrite in meat products |
| KR20010027513A (en) * | 1999-09-14 | 2001-04-06 | 신성수 | manufacture method of fodder |
| KR100384368B1 (en) * | 2001-01-31 | 2003-05-22 | 대한제당 주식회사 | Method for Preparing Conjugated Linoleic Acid Composition |
| KR100431906B1 (en) * | 2001-10-16 | 2004-05-17 | 주식회사 애그리브랜드 퓨리나코리아 | Feed increasing CLA content in the milk and manufacturing method thereof |
| KR100889655B1 (en) * | 2006-11-15 | 2009-03-19 | 고려대학교 산학협력단 | Animal Feed Composition containing by-products obtained from purification process of the conjugated linoleic acid |
| WO2016195369A1 (en) * | 2015-06-05 | 2016-12-08 | 윤관식 | Oil and fat composition for feed, and feed additive for growth promotion, livestock feed composition, and livestock breeding method using same |
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