KR100856456B1 - Feed additive composition for immunopotentiating and stock feed comprising the same - Google Patents

Abstract

Livestock feed containing an immunostimulatory feed additive containing monoglycerides of fatty acid is provided to ensure excellent digestibility and to minimize the use of various antibiotics by increasing disease resistance of livestock. An immunostimulatory feed additive contains 40 to 45% by weight of a monoglyceride of fatty acid having 18 carbon atoms or less; 40 to 45% by weight of a diglyceride of fatty acid having 18 carbon atoms or less; and 10 to 20% by weight of a triglyceride of fatty acid having 18 carbon atoms or less. The fatty acid is selected from stearic acid, linoleic acid, linolenic acid, palmitic acid, myristic acid, lauric acid, caproic acid, caprylic acid, capric acid and a mixture thereof. Livestock feed contains 1 to 30% by weight of the immunostimulatory feed additive. The livestock is selected from a pig, cow, chicken and duck.

Description

Feed additive for immune enhancing and livestock feed including the same {FEED ADDITIVE COMPOSITION FOR IMMUNOPOTENTIATING AND STOCK FEED COMPRISING THE SAME}

The present invention relates to feed additives for enhancing immunity and livestock feed comprising the same. More specifically, the use of antibiotics is increased by increasing the immunity by increasing IgG, which plays an important role in antigen-antibody reactions when feeding livestock. It relates to a feed additive for enhancing immune immunity and animal feed comprising the same.

Livestock such as pigs, cows, and chickens, which are edible animal food sources for humans, are closely fed for mass production. One of the biggest problems in raising livestock is the fact that due to changes in the breeding environment, immunity and physiological activity against diseases and other environmental factors are lowered. This is mainly due to intensive large-scale production, high-density farming, overuse of pharmaceuticals, increased use of chemicals in feedstocks, and increased feedstuffs.

The resistance to livestock disease, i.e., lowering immunity and physiological activity, leads to stagnation of growth and mortality, and increases the amount of antibiotics used to prevent them. This, in turn, has a significant impact on the productivity and economics of livestock farmers.

Accordingly, there is a need for the development of feed additives that can prevent the harmful effects of drugs, such as antibiotics, and to increase the immunity of livestock by replacing antibiotics.

Korean Patent Publication No. 2007-31815 discloses a feed additive consisting of 95% biotite and 5% sulfur containing vanadium and germanium to enhance the immunity of livestock.

In addition, the Republic of Korea Patent Registration No. 10-0554214 is a mixture of 85 to 95% by weight of herbaceous coal to silicate minerals selected from biotite, illite, sericite, and fillite 5 to 15% by weight in order to improve the immunity of livestock 5 at room temperature It is proposed a feed additive composition obtained by aging for 30 days.

However, the above-mentioned feed additive is only an additional ingredient in addition to the nutrient source of the feed, and it has not been disclosed about the addition of the feed that can simultaneously increase the immunity while replacing the nutrient source added in the feed.

Therefore, the present inventors have studied feed additives that can replace the nutrient source of the feed and enhance immunity when feeding the livestock. It is confirmed and suggested that the composition can improve the immunity of livestock.

An object of the present invention is to provide a feed additive for immune enhancement that can replace the fats and oils used as an energy source in the feed as well as to enhance the immunity of the livestock to reduce the use of various antibiotics in livestock raising.

It is another object of the present invention to provide a livestock feed comprising the feed additive for enhancing immunity.

In order to achieve the above object, the present invention

a) 40 to 45% by weight of monoglycerides having 18 or less carbon atoms;

b) 40 to 45% by weight of a diglyceride having 18 or less carbon atoms; And

c) 10 to 20% by weight of triglycerides having 18 or less carbon atoms

It provides a feed additive for immune enhancement comprising.

At this time, the constituent fatty acid is one selected from the group consisting of stearic acid, oleic acid, linoleic acid, linolenic acid, palmitic acid, myristic acid, lauric acid, capronic acid, caprylic acid, capric acid and mixtures thereof.

In another aspect, the present invention provides a livestock feed comprising 1 to 30% by weight of the feed additive for immune boosting.

The feed additive according to the present invention has excellent digestibility and can replace oil, which is an energy source in the feed. In addition, it is possible to minimize the use of various antibiotics by increasing the IgG's anti-pathology by increasing the IgG that plays an important role in antigen antibody response when feeding livestock.

Hereinafter, the present invention will be described in more detail.

The feed additive for immune enhancing according to the present invention has a glyceride form and can replace fats and oils used as energy sources of feed. In addition, by newly suggesting a constituent fatty acid of glycerides, IgG is increased when fed to livestock to enhance the immunity of the livestock.

IgG is most commonly found in colostrum as an immune substance that plays an important role in antigen-antibody responses. These IgGs are transported by lymphatic and circulatory systems to neutralize toxins and foreign antigens. In Experimental Example 2, which tested the immune enhancing effect, the experimental diet fed the immune enhancing feed additive of the present invention increased the content of IgG by 10 to 20 times as compared to the control.

Immunity-enhancing feed additives according to the present invention comprises a) monoglycerides having 18 or less carbon atoms; b) diglycerides having 18 or less carbon atoms; And c) triglycerides having up to 18 carbon atoms.

Immunity-enhancing feed additives of the present invention are glyceride compositions comprising monoglycerides, diglycerides and triglycerides. Therefore, it can be used as an energy source instead of the fats and oils added to livestock feed.

-모노글리세라이드에 비해 친수성이 작아 유화력이 상대적으로 약하다. 또한 에너지원으로 사용되는 수율이 높고, 그람 양성균에 대한 항균력을 갖게 한다. The said monoglyceride has a (beta) -monoglyceride as a main component. β-monoglycerides

-The hydrophilicity is relatively low compared to the monoglyceride, and the emulsification power is relatively weak. In addition, the yield used as an energy source is high, and has antimicrobial activity against Gram-positive bacteria.

Such monoglycerides are contained in 40 to 45% by weight relative to the immune enhancing feed additive of the present invention. If the content is less than the above range, the energy yield and the antimicrobial activity are lowered. On the contrary, if the content exceeds the above range, the antimicrobial activity is increased but the digestibility is low.

The diglyceride is based on 1,2-diglyceride and 2,3-diglyceride. These diglycerides are converted into β-monoglycerides by enzymes in livestock and used as an energy source to have antimicrobial activity against Gram-positive bacteria. On the other hand, the degraded fatty acids are used as energy sources, and fatty acids which are not used as energy sources are used to synthesize glycerides or triglycerides and accumulate as fats in the subcutaneous tissue.

Such diglycerides are contained in 40 to 45% by weight relative to the immune enhancing feed additive of the present invention. If the content is less than the above range, the conversion to monoglycerides is small, resulting in low energy yield and antimicrobial activity. On the contrary, if the content exceeds the above range, it causes fat accumulation of livestock, so it is suitably used within the above range.

The triglycerides are contained in an amount of 10 to 20% by weight based on the feed additive for immune boosting of the present invention. If the content is less than the above range, the conversion to monoglycerides is small, resulting in poor energy yield and antimicrobial activity. On the contrary, if the content exceeds the above range, it causes overfat of livestock fat.

At this time, the constituent fatty acids constituting the monoglyceride, diglyceride and triglyceride may be a kind known to have 18 or less carbon atoms, and the kind is not particularly limited in the present invention. Typically, one selected from the group consisting of stearic acid, oleic acid, linoleic acid, linolenic acid, palmitic acid, myristic acid, lauric acid, capronic acid, caprylic acid, capric acid and mixtures thereof is used.

In terms of immune enhancing effects, the constituent fatty acids are those having 12 or less carbon atoms. Typically, one selected from the group consisting of capric acid, caprylic acid, capric acid, lauric acid, and mixtures thereof is used. According to Experimental Example 2, which tested the immune enhancing effect, the increase in IgG was more pronounced in the diet fed the feed additive containing caprylic acid (C8: 0) and capric acid (C12: 0) as constituent fatty acids.

Livestock feed of the present invention comprises 1 to 30% by weight of the feed additive for immune enhancement.

Since the animal feed is different in its composition and manufacturing method according to the type of livestock to be fed, the composition and manufacturing method in the present invention is not particularly limited.

If the content of the immune-enhancing feed additive contained in the livestock feed according to the present invention is less than the above range, it is difficult to expect the use effect, on the contrary, if it exceeds the above range, the nutrition of the livestock can be unbalanced. .

At this time, the livestock is preferably one selected from the group consisting of pigs, cows, chickens and ducks.

Hereinafter, the present invention will be described in detail by the following Examples and Experimental Examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.

Example  1: Preparation of feed additives for immune enhancement

3 liter four-necked flask equipped with a semi-circle paddle type stirrer containing high purity lauric acid (trade name lauric acid 98%, NATURAL OLEOCHEMICALS SDN BHD) 1600 g, glycerin 400 g ) 300mg was added and reacted at 45 ° C. for 5 torr for 8 hours. The enzyme was then filtered off to remove unreacted glycerin and free fatty acids using molecular distillation.

Example  2: Preparation of feed additives for enhancing immunity

1400 g of coconut oil (trade name refined palm oil Lotte Samkang), 600 g of glycerin, and 50 g of lipase (trade name Lipase TLIM, Novozyme) were used to prepare a feed additive in the same manner as in Example 1.

Example  3: Preparation of feed additives for immune enhancement

A feed additive was prepared in the same manner as in Example 1 using 1600 g of medium-chain fatty acid oil (trade name SEFOL Co., Ltd.), 400 g of glycerin, and 50 g of lipase (trade name Lipase TLIM, Novozyme).

The glyceride and constituent fatty acid composition of the feed additive prepared in Examples 1 to 3 are analyzed and shown in Table 1 below.

Example 1 Example 2 Example 3 Glyceride Composition (% Content) Monoglycerides 43 44 42 Diglycerides 45 45 44 Triglycerides 12 11 14 Fatty Acid Composition (% Content) C6: 0 - - - C8: 0 - 5.8 60 C10: 0 1.2 6.5 40 C12: 0 98 51.2 - C14: 0 0.8 17.6 - C16: 0 - 8.5 - C18: 0 - 2.7 - C18: 1 - 6.5 - C18: 2 - 1.2 - C18: 3 - - -

Experimental Example

Experimental Example  1: Digestibility Measurement

In order to determine the effect on the digestibility of the immune supplement feed additive according to the present invention when replacing with an energy source, the sample of Table 2 was added to the test feed at 2% by weight to carry out animal experiments.

Two 120-membered ternary hybrid (DurocxYorkshirexLandrace) weaning pigs were exposed and the weight of the test was 8.60 ± 1.27 kg. 4 replicates per treatment, 5 batches per replicate.

sample Feed additive composition (% by weight) Control 100% soybean oil Comparison 50% coconut oil, 50% soybean oil Experiment Zone 1 Example 1 25%, soybean oil 75% Experiment Zone 2 Example 1 50%, soybean oil 50% Experiment Zone 3 Example 2 50%, soybean oil 50% Experiment Zone 4 Example 3 50%, soybean oil 50%

The specification test was conducted at the experimental animal breeding room of Dankook University, the test feed was allowed to be a vegetarian, and the water was freely eaten by using an automatic water dispenser.

Body weight and feed intake were measured at the beginning of the test, after 2 weeks, and at the end of the test (after 5 weeks) to calculate daily gain, daily feed intake and feed efficiency.

To measure the nutrient digestibility, chromium oxide (Cr ₂ O ₃ ) was added 0.2% by weight in the feed two times after the start of the test and five times after the test. After 7 days of chromium feed, the powder was collected by anal massage. The collected powder was dried at 60 ℃ hot air dryer for 72 hours and then crushed by Wiley mill for analysis. Cr mixed with the general components of feed and labeling was analyzed by the method presented in AOAC (1995). Feed and intracellular fatty acid analysis was conducted at the Konkuk University Animal Resource Analysis Center.

Table 3 shows the daily gain, feed intake and feed efficiency of each test zone.

division Control Comparison Experiment Zone 1 Experiment Zone 2 Experiment Zone 3 Experiment Zone 4 0-14 days Daily weight gain 0.207 0.209 0.231 0.209 0.224 0.223 Daily average feed intake 0.312 0.290 0.303 0.310 0.275 0.338 Feed efficiency 0.663 0.737 0.772 0.683 0.817 0.669 14-35 days Daily weight gain 0.600 0.612 0.630 0.659 0.636 0.662 Daily average feed intake 0.916 0.969 0.833 0.912 0.895 0.923 Feed efficiency 0.655 0.633 0.786 0.727 0.714 0.740 0-35 days Daily weight gain 0.443 0.451 0.470 0.479 0.472 0.486 Daily average feed intake 0.674 0.697 0.621 0.671 0.647 0.689 Feed efficiency 0.656 0.649 0.780 0.717 0.732 0.723

In daily gain, there was no difference between test groups for 0 ~ 14 days, but experimental group 2 and 4 were significantly higher than control group and control group for 14 ~ 35 days (p <0.05). Daily weight gain was significantly higher than that of the control group 4 and the control group and significantly higher than that of the control group (p <0.05). The daily gain increased with the addition of the feed additive according to the invention.

In the daily feed intake, experimental group 4 was significantly higher than control group and experimental group 4 (p <0.05) for 0-14 days, and control and experimental groups for 14-35 days and 0-35 days. 4 showed significantly higher intake than experimental group 1 (p <0.05).

In addition, the feed efficiency was significantly higher (p <0.05) in the experimental group 3 (p <0.05) and the experimental group 1 was significantly higher than the control group and the control group for 14-14 days. (P <0.05), and experimental group 4 was significantly higher than the comparison group. Experimental group 1 showed significantly higher results than the control group and the control group during the entire 0-35 days (p <0.05).

Table 4 shows the dry matter and nitrogen digestibility of each test zone.

division Control Comparison Experiment Zone 1 Experiment Zone 2 Experiment Zone 3 Experiment Zone 4 14 days building 81.10 84.75 82.78 85.04 82.81 83.67 nitrogen 75.25 79.22 77.81 81.66 78.86 78.06 energy 80.66 84.72 81.34 84.09 82.59 82.04 35 days building 75.59 84.39 84.40 85.00 84.29 84.09 nitrogen 67.68 79.00 77.74 79.36 75.79 78.18 energy 79.03 84.89 84.39 85.14 83.88 83.95

The dry extinguishing rate was significantly higher in the control group, experimental group 1, experimental group 2, experimental group 3 and experimental group 4 than control group (p <0.05).

Nitrogen digestion rate was higher in the experimental group 2 than the other groups, and the energy digestion rate was higher than the control group.

The dry matter, nitrogen and energy digestion rate measured at 35 days were significantly higher in the experimental group 2 than the control group and the control group (p <0.05).

Through these results, it can be seen that the feed additive for immune enhancement of the present invention is excellent in building digestion rate, nitrogen digestion rate and energy digestion rate as compared to soybean oil used as an energy source of the conventional feed.

Experimental Example  2: in blood IgG  increase

In order to determine the effect of the weaning pig's growth ability, blood properties, and blood immunological parameters of the feed supplement for immuno-enhancing according to the present invention, the animal test was performed by adding the sample of Table 2 to the test feed at 2% by weight. .

Two 120-membered ternary hybrid (DurocxYorkshirexLandrace) weaning pigs were exposed and the weight of the test was 8.60 ± 1.27 kg. 4 replicates per treatment, 5 batches per replicate.

The specification test was conducted in the experimental animal breeding room of Dankook University, the test feed was allowed to be a vegetarian, and the water was freely eaten by using an automatic water dispenser.

E3 EDTA vacuum tube (Becton Dickinson Vacutainer systems, Granklin Lakes, NJ) in the jugular vein of the same individual at the start of the test, 2 weeks, and at the end of the test (5 weeks) to determine the immunomarker in the blood. After 5ml of blood was collected, the serum obtained after centrifugation at 2,000 × g for 30 minutes at 4 ° C. was used for analysis of blood immunomarkers (WBC, RBC, Lymphocyte, IgG, total protein, albumin). All data were processed by Duncan's multiple range test using the General Linear Model procedure of SAS (1996) to test the significance between the means.

Table 5 below shows the results of IgG measurement in each test pig wean pig blood.

IgG (mg / mL) Control Comparison Experiment Zone 1 Experiment Zone 2 Experiment Zone 3 Experiment Zone 4 0 days 332.50 311.00 342.50 288.50 314.00 303.00 14 days 308.00 312.00 404.50 339.50 350.25 347.75 35 days 352.50 409.50 504.50 464.50 525.75 689.00 Difference 20.00 98.50 162.00 176.0 211.75 386.00

In addition, the experimental group 4 was significantly higher than the control group, the control group and the experimental group 1 (p <0.05) in the IgG content change during the entire test period.

Through these results, it can be seen that in the experimental diet fed the immune-enhancing feed additive of the present invention, the IgG content is increased up to about 20 times compared to the control not fed. Therefore, the feed additive of the present invention can be fed to enhance the immunity of the livestock.

As the immunity is strengthened when feeding the feed additive for boosting immunity of the present invention to the livestock, it is possible to improve the livestock rearing environment, which can greatly contribute to improving the international competitiveness of domestic livestock industry.

Claims (6)

a) 40 to 45% by weight of monoglycerides having 18 or less carbon atoms; b) 40 to 45% by weight of a diglyceride having 18 or less carbon atoms; And c) 10 to 20% by weight of triglycerides having 18 or less carbon atoms Immunity-enhancing feed additive comprising a. The method of claim 1, The constituent fatty acid is one of stearic acid, oleic acid, linoleic acid, linolenic acid, palmitic acid, myristic acid, lauric acid, capronic acid, caprylic acid, capric acid, and mixtures thereof. Feed additives. The method of claim 1, The constituent fatty acid is immunity enhancing feed additives having 12 or less carbon atoms. The method of claim 3, The constituent fatty acid is one of the one selected from the group consisting of capric acid, caprylic acid, capric acid, lauric acid and mixtures thereof. Livestock feed comprising 1 to 30% by weight of the immune additives feed additive according to any one of claims 1 to 4. The method of claim 5, The livestock is a livestock feed that is one selected from the group consisting of pigs, cows, chickens and ducks.