KR101796225B1

KR101796225B1 - Feed additive composition comprising methionine and vitamin E and manufacturing method thereof

Info

Publication number: KR101796225B1
Application number: KR1020150081325A
Authority: KR
Inventors: 김태일; 박성민; 임동현; 최순호; 기광석; 백광수; 홍재수; 유인선; 봉상훈
Original assignee: 대한민국
Priority date: 2015-06-09
Filing date: 2015-06-09
Publication date: 2017-12-01
Also published as: KR20160144760A

Abstract

The present invention provides a feed additive composition for livestock comprising a mixture of methionine and vitamin E as an active ingredient, wherein the mixture is coated with a hardening oil having an iodine value of 1 or less and a melting point of 57 ° C or higher. The feed additive composition comprising the combined methionine and vitamin E coated preparation having increased safety and absorption rate of the present invention is excellent in the feed intake of livestock and is stable to the lower digestive organ without being degraded by microorganisms, So that the effect of improving liver function and milk yield is excellent.

Description

[0001] The present invention relates to a feed additive composition comprising methionine and vitamin E. [0002] Feed additive compositions comprising methionine and vitamin E,

The present invention relates to a feed additive composition and a method for preparing the feed additive composition, and more particularly, to a feed additive composition for improving feed intake comprising methionine protected by rumen and vitamin E and a method for producing the same.

Ruminants, such as cattle, produce energy production, digestion of fiber, production of vitamins and microbial proteins through normal rumen function to supply the nutrients needed by ruminants.

Cows before and after childbirth undergo major changes in the stages of dry milking, labor, paranoid, and fertilization, and the energy and nutritional levels required in the body at each stage are different. During this period (transition period), dietary intake decreases due to physiological stresses such as rapid growth of fetus, delivery, and analogy, resulting in a rapid increase in body fat mobilization and a rapid change in nutrient supply and metabolism of the feed. In addition, the hormonal changes in the body during the preparation for labor, parable and pregnancy, and during the postpartum restoration period, the excessive increase of the compound feeds for the purpose of increasing the milk production was accompanied by the increase of the digestive and metabolic diseases, Which is a direct cause of liver function deterioration and fatty liver development.

Methionine is a liver function enhancer that is contained in very low concentrations in dairy cows and is a restricted amino acid for dairy cow growth and milk yield based on corn with lysine. In addition, vitamin E can not be synthesized by itself and must be supplied through feed, which is involved in the immune system and plays a major role in the physiological activity of livestock.

However, many components, including methionine, are rapidly degraded by bacteria in the rumen, and eventually only a small amount enters the bloodstream. There have been many attempts to solve these problems, but they have not been solved to date.

Korean Patent Publication No. 10-2005-0087917

In order to solve the problems caused by lowered feed intake of livestock, especially diverting cows, the inventors of the present invention completed the present invention by improving the feed intake of livestock and preparing a combined preparation of methionine and vitamin E with increased safety and absorption rate Respectively.

In order to solve the above-mentioned problems, the present invention provides a feed additive composition for livestock comprising a mixture of methionine and vitamin E as an active ingredient, wherein the mixture is coated with a hardening oil having an iodine value of 1 or less and a melting point of 57 ° C or higher .

In the present invention, the mixture of methionine and vitamin E may contain methionine and vitamin E in a weight ratio of 20: 40: 1, preferably 25: 35: 1, more preferably 30: By weight.

In the present invention, the mixture of methionine and vitamin E may be from 12 to 25% by weight, preferably from 15 to 20% by weight, based on the weight of the whole feed additive composition. When a mixture of methionine and vitamin E is contained in an amount less than or equal to the above range, the effect of improving the feed intake of livestock does not occur. Particularly, when the amount is less than the above range, improvement in liver function or milk yield can not be achieved.

The feed additive composition according to the present invention may be a feed additive composition for livestock, and the livestock may be, for example, cattle, horses, sheep, pigs, deer, chickens, ducks, geese, turkeys, Ostriches, pheasants, and other animals specified by the Agriculture, Forestry and Livestock Food Ordinance, and may be preferably ruminants. The ruminant is also referred to as a ruminant, but is not limited to, for example, a camel, a deer, a deer, a giraffe, and a bovine animal.

In the present invention, the ruminant may preferably be a bovine animal, more preferably a dairy cow, more preferably a diverting cow.

In the present invention, the transition period means the end of labor and the beginning of the parable, and usually refers to three weeks before and after the delivery, though not limited thereto. When the cow reaches birth, the fetus in the abdomen grows rapidly and the nutrient requirement increases. However, the dietary intake is greatly reduced by the transition period before labor, and various problems arise. Characterized in that the mixture of methionine and vitamin E of the present invention is contained as an active ingredient and the mixture is coated with a hardening oil having an iodine value of 1 or less and a melting point of 57 ° C or higher, As a composition capable of fundamentally solving the problem of a sharp reduction in the amount of feed intake of the present invention. In addition, the mixture is protected by ruminal protection with calcium salt fatty acid so that it can be stably transported to the lower digestive organs without being decomposed by microorganisms in the rumen and pH and moisture, so that the liver function and / Was confirmed as shown in the following examples.

In the present invention, the hydrogenated oil is also referred to as hydrogenated oil. The fatty oil as the raw material contains many glycerides of unsaturated fatty acids such as oleic acid, linoleic acid and linolenic acid. When hydrogen is added thereto, hydrogen is added to the unsaturated portion, It becomes a glyceride, so it becomes a solid fat. It has no odor, has a higher melting point than the raw oil, and has a low saponification value and low iodine value and is very stable. The hardening oil can be divided into partial hardening oil and extreme hardening oil. Partial hardening oil is produced by adding hydrogen to the raw oil after reaching the desired physical properties. However, the extreme hardening oil is completely saturate in order to prevent the trans- Cure to the state to prevent trans fat.

In the present invention, the iodine value is also referred to as an iodine value or an iodine value, and is one of the characteristics of the oil and the wax. When the sample oil is subjected to halogen, the amount of absorbed halogen is converted into iodine, which is a number expressed as a percentage of the sample.

In the present invention, the hydrogenated oil having an iodine value of 1 or less and a melting point of 57 ° C or higher may be extremely hardened and unique, and examples thereof include a hardened vegetable oil, a hardened corn oil, a hardened cottonseed oil, a hardened peanut oil, Wherein the hardening oil is at least one selected from the group consisting of oil, hardened palm oil, hardened palm stearin oil, hardened sunflower oil and hardened vegetable oil.

The mixture of methionine and vitamin E in the present invention; And the iodine value of 1 or less and melting point of 57 ° C or higher may be contained in the feed additive composition in a mass ratio of 2: 1 - 1: 2, and preferably in a mass ratio of 1.5: 1 - 1.5: 1.

In the present invention, the cured oil having an iodine value of 1 or less and a melting point of 57 ° C or more may be 10 - 20% by weight, preferably 12 - 18% by weight based on the weight of the whole feed additive composition. When the hardening oil is contained in the above range, the coating is not properly performed and the effect of the present invention can not be expected. If the hardening oil is included in the amount exceeding the above range, the effect of improving the feed intake of the livestock can not be improved.

In the present invention, the mixture may be rumen-protected as a calcium salt fatty acid. The present invention is not limited to this, but it is expected that the calcium salt fatty acid can facilitate the absorption of methionine and vitamin E in the lower digestive organs without affecting the rumen microorganism.

The fatty acids in the present invention include, but are not limited to, vegetable fats such as soybean oil, corn oil, olive oil, cottonseed oil, or palm oil; Or processed animal fats such as fish oil, tallow, lard, etc., and may be preferably palm fatty acid.

The present invention also provides a method for preparing a feed additive composition for a domestic animal comprising the steps of:

S1) adding calcium salt fatty acid with methionine and vitamin E at a weight ratio of 20 - 40: 1 and mixing;

S2) coating with addition of hardened oil having an iodine value of 1 or less and a melting point of 57 ° C or higher; And

S3) injecting the mixture.

In the present invention, the calcium salt fatty acid may be prepared by adding calcium hydroxide to the fatty acid before the step S1). The fatty acid may include, but is not limited to, vegetable fats such as soybean oil, corn oil, olive oil, cottonseed oil or palm oil; Or processed animal fats such as fish oil, tallow, lard, etc., and may be preferably palm fatty acid.

Palm fatty acid is palm oil processed and fat oxidation. Unlike palm oil, it exists in solid state at normal temperature.

Palm oil is a pure vegetable natural oil that is extracted hygienically from pure pear clusters in the fruit pine tree of the palm tree and belongs to the palm tree. Since it is the fat derived from the plant, there is no concern about cholesterol and the chemical treatments harmful to the human body such as hydrogenation It is possible to perform various applications with minimal chemical treatment.

The step of adding calcium hydroxide to the fatty acid is not limited thereto, but may be added in a water bath. The mixture formed through the addition may be dried to produce a calcium salt fatty acid.

In the present invention, methionine and vitamin E in step S2) may be added in a weight ratio of 25: 35: 1, more preferably 30: 1.

In the step S2) of the present invention, a filler may be further added, and the filler may be added with materials widely used in the art, for example, but not limited to, calcium carbonate.

In the present invention, the hydrogenated oil of step S2) may have an iodine value of 1 or less and a melting point of 57 ° C or more.

The present invention also provides a livestock feed comprising the composition.

The present invention also provides a method for raising livestock comprising feeding the livestock feed to the livestock.

In the present invention, the livestock feed can be fed for 10 to 20 days before delivery.

The feed additive composition comprising the combined methionine and vitamin E coated preparation having increased safety and absorption rate of the present invention is excellent in the feed intake of livestock and is stable to the lower digestive organ without being degraded by microorganisms, So that the effect of improving liver function and milk yield is excellent.

FIG. 1 is a graph showing changes in blood free fatty acid (NEFA) of the combination preparation of the present invention and a control. FIG.
FIG. 2 is a graph comparing the effects of improving the feed intake of the combination preparation of the present invention and the control.
FIG. 3 is a graph comparing the effect of increasing the flow rate of the combination preparation of the present invention and the control. FIG.

Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

< Example 1>

Manufacture of methionine and vitamin E for rumen protection

Preparation of calcium salt fatty acid

To prepare rumen protected methionine and vitamin E, calcium salt fatty acid, a protective fat, was first prepared. Calcium hydroxide was added to the fatty acid derived from vegetable palm oil and reacted in a water bath to remove water by centrifugation and dried well to prepare calcium salt palm fatty acid (calcium salt protective fat).

Manufacture of methionine and vitamin E for rumen protection

16.7 units of methionine (99% content) and 1.3 units of vitamin E (50% content) were added to a super mixer and mixed at 600 rpm for 5 minutes in 57 units of calcium salt protective fat prepared as described above. Then, 10 units of calcium carbonate (99%) as a filler and 15 units of extreme hardening oil (iodine less than 1.0 and melting point of 57 ° C or more) as a binding coating agent were further added to a super mixer and mixed at 700 rpm for 7 minutes. The thus homogeneously mixed mixture was extruded through a extruder into a 1.4 mm diameter perforated plate. At the time of injection, the temperature of the perforated plate was made to be 55 ° C or more, and the injection product was cooled to below 10 ° C to be cooled immediately. Immediately, the cooled injection was transferred to a high speed mixer so that the bonding coating could cover the surface evenly. The mixture was rapidly mixed for 10 minutes at an agitator of 80 rpm and a chopper of 1200 rpm, cooled for 10 minutes, The prototype was prepared by filtering.

< Example 2>

General composition of feed

Analysis item name Name of sample TMR for dry organic matter TMR for home General component moisture(%) 46.45 38.00 Crude protein (%) 6.97 10.93 Crude fat (%) 2.52 3.32 Crude fiber (%) 13.19 11.57 Views min (%) 4.25 4.90 ADF (%) 14.57 13.15 NDF (%) 26.25 23.74

As shown in Table 1 above, TMR (Total Mixed Ration) consisting of 6.97% crude protein and 10.93% lactated milk was used as a standard feed for the test shafts. 500 g of concentrated feed was fed to the test shaft at 9:30 am, and 50 g of a mixture of methionine and vitamin E was added to the treatments.

< Example 3>

Free fatty acids in blood ( NEFA ) Change

division Treatment Control Compound preparation Free fatty acids (uEq / L) Number of repeats Free fatty acids (uEq / L) Number of repeats 14 days before delivery 353.5 6 351 7 7 days before delivery 392 5 362.5 6 0 days before delivery 414 5 339 6

The cow diverticulum is a stage where the liver function is deteriorated due to the physiological characteristics and the disease is accelerated along with the lowering of the immune function after the delivery due to the lowering of the liver function. Thus, the combination preparation of the present invention reduces the amount of body fat mobilized by energy imbalance, , And thus contributes to the cow's robustness through the improvement of the liver function, the increase of the feed intake, the increase of the flow rate and the strengthening of the immunity. As shown in Table 2 and FIG. 1, in the untreated control preparation, 17.11% was increased from 353.5 uEq / L at 14 days before delivery to 414 uEq / L at the day of delivery, indicating that body fat was mobilized, At 14 days of labor, serum free fatty acid concentration was decreased from 351 uEq / L to 339 uEq / L on the day of delivery to 3.42%, indicating that stress was prevented and liver function was improved.

< Example 4>

Confirmation of improvement of feed intake

The present inventors confirmed that the combination preparation of the present invention had an effect of improving the feed intake level by feeding the combination of methionine and vitamin E for 14 days before delivery.

division Treatment Control Compound preparation Average intake (kg) Number of repeats Average intake (kg) Number of repeats 0 to 7 days before delivery 12.4 ± 6.1 6 19.4 ± 9.0 7 8 to 14 days before delivery 14.4 ± 5.4 5 18.4 ± 5.8 6

As shown in Table 3 and FIG. 2, the feed intake level of the combination of methionine and vitamin E was decreased by about 13.8% in the control group (14.4 kg / d (2 weeks) → 12.4 (1 week)). In the feeding group, the increase was 5.4% (18.4 kg / d for 2 weeks and 19.4 for 1 week). The average daily feed intake for the week before labor was 12.4 kg / d in the control group and 19.4 kg / d in the feeding group, which was 56%.

< Example 5>

Indirect effects after formulation

The inventors of the present invention have confirmed the effect of increasing the flow rate as an indirect effect after the administration of the combination preparation of the present invention.

division Treatment Control Compound preparation Average flow rate (kg) Number of repeats Average flow rate (kg) Number of repeats 1 ~ 20 days analogy (1) 23.3 ± 7.5 6 25.2 ± 8.5 7 20 ~ 40 days analogy (2) 26.7 ± 4.6 5 31.1 ± 4.2 7 41 ~ 60 days analogy (3 28.9 ± 3.7 4 33.9 ± 5.3 7

A combination of methionine and vitamin E was administered daily at 50 g of the formulation for 14 days before delivery. The average daily flow rate during 20 days after delivery was 25.2 kg, which was 8.2% improvement compared to the control. The mean flow rate during 20 days thereafter was also improved by 16.5% to 31.1kg. In the third period, the average flow rate was improved by 17.3% to 33.9kg, indicating that the physiological activity effects of methionine and vitamin E preparations were maintained.

< Example 6>

Economic analysis

Estimates of the economic benefits resulting from feeding the combination of the present invention to cows with feed additives are as follows.

Hand performance factor (A) Benefit Factor (B) * Increased costs
- Additive: 91 days X 50g X 5,000 won / 1000g
- Total (A): 22,750 won
- Feed ratio: (7.03kg / two X 7 days X 7 two X 388 won / kg) + (3.98 kg / two X 7 days X 6 two X 388 won / kg) = 133,654 won + 64,858 won = 198,512 won
- Total (A): 221,262 won * Increased profits
- Additional milk production: 1.9kg / two
- Bond: 1.9kg / two X 7 two X 20 days X 1092.4 yuan = 292,657 yuan

- Total (B): 292,657 won * Estimated profit (B-A) = 292,657 won - 221,262 won = 71395 won / 7 won = 10,199 won / two

- Bond: 1092.39 won (Bond value applied in the first half of August 2014)

- TMR feed rate for dry organic matter: 388 won / kg (2014 livestock source)

As described above, the estimated profit is about 10,199 won / yuan, which is higher than the conventional profit, and it is confirmed that the use of the feed additive composition of the present invention is advantageous in generating economic profit as compared with the prior art.

Claims

Methionine and vitamin E as an active ingredient and the mixture is coated with a hardening oil having an iodine value of 20 or less and a melting point of 57 ° C or higher and the mixture of methionine and vitamin E is added in an amount of 15-20 weight By weight of the feed additive composition for improving the feed intake, liver function and milk yield of the diverticulum cow.

The feed additive composition for a domestic animal according to claim 1, wherein the iodine value is 1 or less.

The composition of claim 1, wherein the mixture of methionine and vitamin E comprises methionine and vitamin E in a weight ratio of 20-40: 1.

2. The feed additive composition according to claim 1, wherein the mixture of methionine and vitamin E comprises methionine and vitamin E in a weight ratio of 25-35: 1.

delete

2. The feed additive composition according to claim 1, wherein the mixture is rumen-protected with calcium salt fatty acid.

delete

A method for preparing a feed additive composition for livestock for improving feed intake, improving liver function and milk yield of diverting cows, comprising the steps of:
S1) adding calcium methionine and vitamin E to a calcium salt fatty acid at a weight ratio of 20-40: 1, adding a mixture of methionine and vitamin E to the total feed additive composition in an amount of 15-20% by weight;
S2) coating with addition of hardened oil having an iodine value of 20 or less and a melting point of 57 ° C or higher; And
S3) injecting the mixture.

11. The method of claim 10, wherein the iodine value in step S2) is 1 or less.

[Claim 11] The method according to claim 10, wherein methionine and vitamin E in step S1) are added in a weight ratio of 25: 35: 1.

[Claim 11] The method of claim 10, wherein calcium carbonate is further added as a filler in step S2).

A livestock feed comprising the composition of any one of claims 1 to 4 and 7.

A method of raising livestock comprising feeding a composition according to any one of claims 1 to 4 to a livestock.

16. The method of claim 15, wherein the livestock feed is fed for 10-20 days before delivery.