KR100431906B1 - Feed increasing CLA content in the milk and manufacturing method thereof - Google Patents

Abstract

The present invention relates to processed corn, wheat, jade by-products, defatted steel, soybean hulls, lupine, flour, wheat bran, vegetable lime, limestone, defluorinated phosphoric acid, salt, molasses, mineral additives, linolein, which is a precursor of CLA to vitamin additives. As a vegetable fat-rich ingredient rich in acid and linolenic acid, the whole feed supplemented with heat-treated whole soybeans alone or mixed with heat-treated soybeans and linseed oil was heat-treated at a high temperature for a short time and fed to 18 soybeans, and then fed into milk fat. After analyzing the content and CLA content and analyzing the change in the CLA content, the feed containing only the above-mentioned heat-treated soybeans was fed to 14 cows and raised, and then the amount of milk components, fatty acid composition and CLA content in milk were analyzed. Short-term heat treatment at high temperature after adding soybeans increases the CLA content in milk without reducing milk fat and flow rate. It has an excellent effect on preparing feed.

Description

Feed increasing CLA content in the milk and manufacturing method

The present invention relates to a feed for increasing the CLA content in milk and a method of manufacturing the same. More specifically, the present invention is a feed suitable for normal digestive physiology of cows that increase the CLA content in milk without reducing milk fat and flow rate by short-term heat treatment of the whole milking cow milk made with the addition of heat-treated whole soybean and linseed oil at high temperature It relates to a manufacturing method.

Conjugated linoleic acid (CLA) has anticancer effects (Shultz et al. 1992; Visonneau et al. 1997), antioxidant effects (Ha et al., 1998), immunopotentiation effect (Milller et al. 1994), body fat reduction and body protein retention. It is known to have a function such as effect (Thom et al., 1997). It is structurally 18 carbon atoms in which two unsaturated bonds (between C9 and C10, between C12 and C13) are different from linoleic acid in geometric or positional cis and trans. Refers to a mixture containing isomers (eg c-9, t-11; t-10, C-12; t-9, t-11; and t-10, t-12-octadecadienoic acid). These CLAs are produced as intermediates of the hydrogenation of linoleic acid by Butyrivibrio fibrisolvens bacteria in the rumen, and have recently been reported to be transcribed in mammary cells. Present and its content is 0.3-0.6% of milk fat, 2.5-6 mg / g milk fat, but in order to see the effect of CLA, it is recognized as a functional food (Kepler 1967). For this reason, the method of increasing the CLA content in milk is a method by controlling the ratio of grazing and forage and rich feed (Dhiman et al., 1996) and the content of linoleic acid or linolenic acid. Feeding cows with feed containing abundant vegetable oil has been reported to be effective (Dhiman et al., 2000). However, feeding vegetable-free oil with high unsaturation inhibits the activity of microorganisms in the rumen and prevents fibrinogen. Digestion rate is reduced due to the coating of (Kowalczyk et al., 1977), reducing the absorption of divalent ions from the small intestine (Tillman and Brethour, 1958) as well as a decrease in fat content (Nicholson et al., 1971). That is, the addition of a large amount of unsaturated vegetable oil to the cow's feed induces an increase in the CLA content in the milk but is not suitable for the normal digestive physiological activity of the cow can also be a cause for poor oil quality. In addition, increasing the amount of vegetable oil in the form of unprocessed grains in feed rather than in the form of free oil does not significantly affect the content of CLA in milk (Dhiman et al. 2000). Therefore, in order to increase the CLA in cow's milk by increasing vegetable oil rich in linoleic acid or linolenic acid, it should not affect the digestive physiology and oil quality of cow's milk. In terms of use, technology development is required to maximize the conversion efficiency to CLA.

In view of the above, the inventors of the present invention processed corn, wheat, jade by-products, skim steel, soybean hull, lupine, wheat flour, wheat bran, vegetable gourd, limestone, defluorinated phosphate, salt, molasses, mineral additives, vitamin additives After the addition of heat-treated whole soybean, which is a vegetable oil, or a mixture of heat-treated whole soybean and linseed oil, the whole feed is heat-treated at a high temperature for a short time to prepare a feed, and the feed is fed to 18 soybeans, and the milk yield is increased. The present invention was completed by analyzing the milk fat and CLA content and feeding the heat-treated whole soybean meal to 14 dogs, and analyzing the change of dairy components, fatty acid composition and CLA content.

Accordingly, an object of the present invention is to maintain vegetable in processed corn, wheat, jade by-products, skimmed steel, soybean hulls, lupine, flour, wheat bran, vegetable lime, limestone, defluorinated phosphate, salt, molasses, mineral additives, vitamin additives. The present invention provides a method for producing a feed which increases the CLA content in milk after heat-treating whole soybeans as raw materials alone or by mixing heat-treated soybeans and linseed oil at high temperature for a short time. Other objects of the present invention are vegetable fats and oils in processed corn, wheat, jade processed by-products, skimmed steel, soybean hulls, lupine, flour, wheat bran, vegetable gourd, limestone, defluorinated phosphate, salt, molasses, mineral additives and vitamin additives. Phosphorus heat-treated whole soybeans alone or a mixture of heat-treated soybeans and linseed oil is added to provide a feed that increases the CLA content in milk without shortening the milk fat and flow rate by heat treatment for a short time at high temperature.

The above object of the present invention is a heat treated battery soybean in processed corn, wheat, jade by-products, skim steel, soybean hulls, lupine, flour, wheat bran, vegetable gourd, limestone, defluorinated phosphate, salt, molasses, mineral additives, vitamin additives The whole feed added alone or mixed with heat-treated whole soybean and linseed oil was heat-treated at a high temperature for a short time and fed the feed to 18 cows, and analyzed the yield, milk fat and CLA content. This was achieved by feeding the diet to 14 cows and analyzing the changes of dairy components, fatty acid composition and CLA content.

Hereinafter, the configuration of the present invention.

The present invention is a processed corn, wheat, jade by-products, degreasing steel, soybean hull, lupine, flour, wheat bran, vegetable greens, limestone, defluorinated phosphoric acid, salt, molasses, mineral additives, vitamin additives alone heat-treated whole soybeans Or preparing a feed mixture by mixing heat-treated whole soybean and linseed oil; Feeding the feed prepared in the above step to 18 head siwoo breeding for 8 weeks; Analyzing the milk fat content and the CLA content in milk collected from 18 head gongwoo beef bred in the above step; Feeding the feed containing only the heat-treated whole soybeans for four weeks to 14 heads of cows; It is composed of a step of analyzing the amount of milk components, fatty acid composition and CLA content in the milk collected from 14 head gongwoo beef bred in the above step.

In the present invention, the heat-treated soybean (ESB), linseed oil (LO), which is about twice as rich in linoleic acid or linolenic acid in the feed than conventional feed, contains linseed oil (LO). A high-temperature micro time (HTMT) feed method that combines one or two high-fat feeds and processes 0.2-0.4 seconds at 120-150 ° C to gelatinize 60-80% starch in the feed to 1.5 % Feed fat is bound-fat (mainly solid fat condensed with viscous substances such as starch) to maximize feed and feed through individual specification management by automatic feeding. During the week, the CLA content in milk, milk fat and cow's milk was measured, and then the feed of good results was selected to re-verify the CLA content in milk and to investigate the fatty acid composition. In order to have statistical reliability, the significance of the mean value of the treatment interval was used by the general lineal model of SAS (1995), and the significance test was Duncan's multiple analysis method (Duncan, 1995). And student's T-test. On the other hand, the heat treatment cell soybean is heat treated to remove the activity of trypsin inhibitor, an anti-nutritional factor contained in soybean, without squeezing oil from soybean. Refers to a protein feed raw material that has undergone a process, in the present invention, soybean, which has been subjected to heat treatment for 2 to 3 minutes at about 80 to 90 ° C. by an extrusion method, which is a conventional heat treatment method, is used. The heat treatment process was carried out by the trucion process. The petroleum by-products were produced after obtaining starch, sugar, oils, etc. from corn. Are by-products such as protein peel, gluten free, ox germ gourd, and vegetable gourds are squeezed vegetable oils from seeds and include soybean meal, rapeseed gourd, palm gourd, cottonseed gourd, and flax gourd. The jade processed by-products and vegetable greens may be used as long as they correspond to them, and may be used alone or in combination. Mineral additives may be used to supply minerals such as calcium, phosphorus, magnesium, potassium, sodium, selenium, and sulfur. As an additive to be added for the sake, any one may be used, but in the present invention, a MyMax product sold by Shinhan Livestock Co., Ltd. was purchased and used. Vitamin additives may also be used in any of the products, in the present invention was used to purchase Vitaton, Samwoo Median Co., Ltd. In addition, defluorinated phosphate means one of the phosphorus (P) source from which the fluorine source is removed, lupine Blooming dicotyledon is a bark of lupine, which is a genus of lupine in the genus Lipanaceae. Wheat vein refers to wheat, ie, the husk of wheat.

The composition ratio of the feed of the present invention is 7-25% by weight of heat-treated whole soybean, 7-16% by weight of processed corn, 6-12% by weight of wheat, 7-18% by weight of jade by-products, 0.01-3% by weight of skim steel, soybean hulls 6-9% by weight, 3-7% by weight of lupine, 1-5% by weight of wheat flour, 1-7% by weight of wheat, 17-23% by weight of vegetable greens, 1-2% by weight of limestone, 0.1-1.0% by weight of fluorinated phosphate %, 0.5 to 1.5% by weight of molasses, 5 to 7% by weight of molasses, 0.1 to 0.2% by weight of mineral additives, 0.01 to 0.1% by weight of vitamin additives, and particularly preferably 15% by weight of heat-treated soybeans.

Hereinafter, the specific method of the present invention will be described step by step with reference to Examples, but the scope of the present invention is not limited only to these Examples.

Example 1 Feed Preparation Containing ESB and LO

In the present invention, a commercial feed custom mix (CM) and two kinds of feeds designed by themselves were used. A feed was prepared by HTMT treatment after the addition of 8.3% ESB and 0.9% LO, and B feed was prepared by HTMT treatment after the addition of 15% ESB. The composition of the test feed is shown in Table 1, and the general components of each feed are analyzed and shown in Table 2 and Table 3, respectively. Total Digestible Nutrients (TDN) was the total amount of plasticized nutrients. The control group was 73.10% by weight of the total feed, feed A was 73.03%, and feed B was 73.29% by weight.

Composition of test feed (%) Control Feed A Feed B Processed corn 14.00 14.26 12.45 Heat Treated Battery Soybean (ESB) 0.00 8.30 15.00 wheat 10.00 10.00 10.00 Jade processing by-product 19.15 17.70 10.50 Degreasing Steel 1.98 0.00 3.00 Soybean Scalp 7.65 7.81 7.61 Lupine 7.00 5.00 5.00 flour 3.00 3.00 3.00 Wheat Mace 1.50 1.92 6.97 Vegetable greens 24.80 21.38 16.95 Limestone 1.07 1.21 1.32 Defluorinated Phosphate 0.78 0.66 0.60 Salt 1.00 1.00 1.00 Flaxseed oil (LO) 0.00 0.90 0.00 molasses 6.00 6.00 6.00 Mineral additives 0.16 0.16 0.16 Vitamin additives 0.06 0.06 0.06 Etc 0.25 0.65 0.38 all 100.00 100.00 100.00

General component I of test feed (%) Control Feed A Feed B moisture 11.00 11.00 11.00 Crude protein 19.70 19.70 19.70 Crude fat 4.40 4.31 4.56 Crude fiber 10.50 10.50 10.50 Ash 7.26 7.23 7.26 calcium 0.90 0.90 0.90 sign 0.60 0.60 0.60 sulfur 0.25 0.25 0.25 magnesium 0.35 0.33 0.35 potassium 1.10 1.00 1.09 Selenium 0.38 0.38 0.34 Salt 1.00 1.00 1.00 Breakdown protein 13.45 13.45 13.50

General Ingredients II of Test Feed (%) Control Feed A Feed B Undigested protein 6.21 6.25 6.20 ADF 13.60 13.55 13.74 NDF 23.70 22.70 23.93 Effective fiber 0.29 0.29 0.29 Starch 0.78 0.78 0.79 Lignin 0.11 0.11 0.11 Unstructured Carbohydrates 35.00 35.00 35.00

NDF (neutral detergent fiber): neutral detergent insoluble fiber

ADF (acid detergent fiber): acid detergent insoluble fiber

Example 2 Breeding of 18 Head Gongwoo

For the present invention, after eight weeks of feeding a custom mix feed for 18 weeks on the market, the whole diet was divided into two test plots (feed A, feed B) in consideration of the fertilization time, acid difference, flow rate and milk composition. Treatment 2 with feed A was gradually replaced with feed B for 2 weeks and continued for 6 weeks. The diet was fed based on the NRC (2000) nutrient recommendation during the entire test period. The ratio of rich feed and forage was 50:50. As for the fee for use, a toll pass cue was used. Milk samples were collected once (morning and afternoon) before feed replacement and once every 7 days (morning and afternoon) after the complete change of feed. Feed intake was recorded daily to investigate the average daily dry intake for the entire test period.

Example 3: Analysis of milk fat content and CLA content after breeding 18 head gongwoo

The milk fat content and CLA content of the milk sample collected in Example 2 were analyzed. Feed intakes of treatments 1 and 2 were 23.73 and 24.44 kg, respectively, during the entire test period of Example 2, which was not statistically significant with 24.44 kg before the start of the test (P <0.05). There was no statistical significance in treatment 1 and 2, 30.11, 29.85 and 30.63 kg, respectively. The milk fat content was 3.80, 3.70, and 3.54%, respectively, but there was no statistical significance, but the CLA content in oil was 5.90, 7.46, and 10.45mg / g milk fat, respectively. Significantly increased (P <0.05) and Treatment 1 showed a tendency to increase compared to the start of the test (Table 4).

Effects of Feed Processing with Different Fat Ingredients on Feed Intake and Dairy Components Item Control Processing 1 (feed A) Processing 2 (feed B) Building Intake (kg / day) 24.64 23.73 24.44 Milk yield (kg / day) 30.11 ± 2.11 29.85 ± 1.61 30.63 ± 1.64 Fat percentage (%) 3.80 ± 0.14 3.70 ± 0.11 3.54 ± 0.10 CLA (mg / g fat) 5.90 ± 0.67 ^b 7.46 ± 0.58 ^ab 10.45 ± 0.61 ^a

1. The control group was the mean ± standard error value of 18 heads before treatment, and the values of treatments 1 and 2 were shown as the mean values for 8 weeks.

2.a, b P <0.05

Example 4 Changes in CLA Content during 18-Double Grow Breeding

The milk yield, milk fat and CLA content for 8 weeks obtained as a result of the experiment of Example 2 are shown in Tables 5, 6 and 7.

Changes in Milk Production According to Salary of Invention Feed Oil yield (kg / day) 0 weeks 2 weeks 3 weeks 4 weeks 6 Weeks 8 Weeks Processing 1 (feed A) 30.0 ± 2.9 29.3 ± 2.9 29.2 ± 3.3 29.2 ± 3.2 30.18 ± 3.53 28.04 ± 3.36 Processing 2 (feed B) 32.8 ± 2.5 35.6 ± 3.3 34.3 ± 3.1 33.7 ± 3.0 30.30 ± 2.75 29.00 ± 3.16

Changes in Milk Fat Contents According to Feeding of Invention Fat (%) 0 weeks 2 weeks 3 weeks 4 weeks 6 Weeks 8 Weeks Processing 1 (feed A) 3.83 ± 0.2 3.93 ± 0.24 3.69 ± 0.17 3.72 ± 0.15 3.82 ± 0.25 3.90 ± 0.35 Processing 2 (feed B) 3.81 ± 0.23 3.90 ± 0.21 3.62 ± 0.23 3.77 ± 0.21 3.79 ± 0.19 3.85 ± 0.26

Changes in CLA Contents in Milk According to Feeding of Invention CLA content (mg / g fat) 0 weeks 2 weeks 3 weeks 4 weeks 6 Weeks 8 Weeks Processing 1 (feed A) 6.03 ± 1.26 7.15 ± 0.84 6.8 ± 1.18 7.59 ± 0.86 7.56 ± 0.42 8.21 ± 0.76 Processing 2 (feed B) 5.90 ± 0.92 10.30 ± 0.72 10.4 ± 1.0 10.7 ± 1.03 11.06 ± 0.65 11.85 ± 0.52

As a result, the milk production and milk fat content showed no significant change compared to the pre-treatment (0 week) in both feed A and B, but the CLA content in water increased after feeding A and B, especially 15% ESB. It can be seen that the addition of HTMT-treated feed B (Treatment 2) shows a significant increase of about 2 times compared to before treatment.

Example 5 14 Head Gongwoo Breeding

After the addition of 15% ESB to the commercial custom mix feed and the custom mix feed used in Example 2 using 14 two sieves, it was performed for 4 weeks using HTMT-treated feed B (treatment 3). Feed was fed based on NRC (2000) nutrient recommendations during the entire breeding (see Tables 8 and 9). Milk samples were taken three times (morning and afternoon) for 7 days before feed replacement and three times (morning and afternoon) for 7 days before completion of test.

Composition of feed used for breeding (%) Control Treatment CM 55.78 0 Feed B 0 56.00 Alfalfa 8.54 12.01 TMR 29.99 15.06 Tall Pesque Hay 5.69 8.01 WCS 0 8.92

TMR: Total mixed ration

WCS: whole cotton seed

General composition of feed used for breeding (%) Control Treatment moisture 12.07 12.04 TDN 69.79 70.74 Crude protein 16.63 17.23 Crude fat 2.46 4.74 RUP 5.10 6.16 NDF 32.49 34.38 ADF 18.76 20.81 calcium 0.81 0.83 sign 0.68 0.49

TDN: total digestible nutrients

RUP: rumen undegradable protein

NDF: neutral detergent fiber

ADF: acid detergent fiber

Example 6 Analysis of the Amount, Fatty Acid Composition and CLA Content of Milk Components after Breeding of 14 Heads of Gongwoo

As shown in Example 5, the feed intake and the amount of dairy components measured after breeding fourteen cows for four weeks are shown in Table 10.

Changes in dairy components Item Control Processing 3 Fat yield (kg / day) 0.97 ± 0.08 0.87 ± 0.11 protein(%) 3.54 ± 0.06 3.49 ± 0.10 Lactose (%) 4.65 ± 0.07 4.77 ± 0.08 TS (%) 13.32 ± 0.30 12.84 ± 0.34 SNF (%) 8.89 ± 0.13 9.07 ± 0.27

TS (Total Solid): Total Solids

Soiled non fat (SNF)

The fatty acid composition and CLA content measured after breeding fourteen cows for four weeks as in Example 5 are shown in Table 11.

Fatty Acid Composition and CLA Content in Milk (mg / g) Item Control Processing 3 C _{4: 0} 14.25 ± 1.07 16.66 ± 0.83 C _{6: 0} 14.22 ± 0.58 13.80 ± 0.80 C _{8: 0} 12.21 ± 0.57 11.69 ± 3.91 C _{10: 0} 32.04 ± 1.67 30.21 ± 3.57 C _{12: 0} 45.56 ± 2.16 41.94 ± 2.30 C _{13: 0} 0.86 ± 0.12 1.08 ± 0.62 C _{14: 0} 118.82 ± 2.66 112.79 ± 2.43 C _{14: 1} 11.12 ± 1.54 9.36 ± 0.97 C _{15: 0} 9.62 ± 0.25 8.34 ± 0.32 C _{16: 0} 298.54 ± 5.66 265.90 ± 6.98 C _{16: 1} 17.66 ± 2.58 12.69 ± 1.56 C _{17: 0} 6.09 ± 0.16 4.40 ± 0.14 C _{17: 1} 2.28 ± 0.11 0.78 ± 0.28 C _{18: 0} 124.46 ± 4.45 131.91 ± 12.95 cis-C _{18: 1} 228.93 ± 7.53 241.01 ± 13.34 trans-C _{18: 1} 18.46 ± 0.66 31.88 ± 1.44 C _{18: 2} 35.50 ± 1.33 48.84 ± 2.86 C _{18: 3} 3.30 ± 0.10 7.24 ± 0.42 CLA 5.37 ± 0.25 9.48 ± 0.70

As can be seen from the above experimental results, the feed intake and dairy components did not change when the feed was added to ESB 15% by weight (treatment), but there was a marked increase in the CLA content in milk. Especially in the composition of fatty acid in milk, the content of cis-C _{18: 1} , trans-C _{18: 1} , C _{18: 2} and C _{18: 3 in} the oil was increased significantly.

As described above, the feed heat-treated for a short time at high temperature by the addition of the heat-treated battery soybean of the present invention has an excellent effect suitable for normal digestive physiology of cows that increase the CLA content in milk without reducing milk fat and flow rate. This is a very useful invention in the dairy industry and dairy industry.

Claims (2)

Processed corn 7-16%, wheat 6-12%, jade by-products 7-18%, skim steel 0.01-3%, soybean hull 6-9%, lupine 3-7%, flour 1 -5% by weight, 1-7% by weight of wheat, 17-23% by weight vegetable vegetation, 1-2% by weight of limestone, 0.1-1.0% by weight of defluorinated phosphoric acid, 0.5-1.5% by weight of salt, 5-7% by weight of molasses, 0.1 to 0.2% by weight of mineral additives and 0.01 to 0.1% by weight of vitamin additives are mixed with 7 to 25% by weight of heat-treated battery soybeans at 120 to 150 ° C for 0.2 to 0.4 seconds, without reducing milk fat and flow rate. A method of preparing a feed to increase the content of linoleic acid (CLA) in the milk. A feed prepared by the method of claim 1.