KR20170010637A - Feed compositions for production of milk comprising improved ratio of fatty acids and omega-3,6 - Google Patents

Feed compositions for production of milk comprising improved ratio of fatty acids and omega-3,6 Download PDF

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KR20170010637A
KR20170010637A KR1020150102531A KR20150102531A KR20170010637A KR 20170010637 A KR20170010637 A KR 20170010637A KR 1020150102531 A KR1020150102531 A KR 1020150102531A KR 20150102531 A KR20150102531 A KR 20150102531A KR 20170010637 A KR20170010637 A KR 20170010637A
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weight
omega
milk
composition
feed
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Korean (ko)
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강호진
정다혜
김동국
김은아
신용국
김원
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서울우유협동조합
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/158Fatty acids; Fats; Products containing oils or fats
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/18Lipids
    • A23V2250/186Fatty acids

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Birds (AREA)
  • Fodder In General (AREA)

Abstract

The present invention relates to a feed composition for producing milk having an improved ratio of fatty acids and omega-3 and 6 and, more specifically, to a milking cow feed composition for producing milk having an improved ratio of functional fatty acids and omega-3 and 6, comprising 2 to 4 wt% of linseed, canola seed, and fish oil as fatty acid sources, and 2 to 4 wt% of sorghum, millet, and pea as excipients.

Description

[0001] The present invention relates to a feed composition for producing milk having improved fatty acid content and a ratio of omega-3, omega-6,

The present invention relates to a feed composition for producing milk having improved fatty acid content and omega-3, 6 ratio, and more particularly to a feed composition comprising 2 to 4% by weight of flaxseed, canola and fish oil as fatty acid sources, And 2 to 4% by weight of peas. The present invention relates to a dairy composition for producing milk with improved functional fatty acid content and a ratio of omega-3 to omega-6.

One 200 mL of milk contains 8 g of fat and 5 g of saturated fat, which corresponds to 16% and 33% of the daily nutrient standard. Excessive intake of saturated fats can be a cause of various adult diseases, making it an excuse for people who do not like to drink milk. High fat omega-6 fatty acids increase heart disease, cancer, inflammation, autoimmune disease, and, on the other hand, higher omega-3 fatty acids (Simopoulos, 2002)

Fatty acid composition of milk can be changed according to the diet, and milk with docosahexaenoic acid (DHA), conjugated linoleic acid (CLA) and omega-3 composition is sold. According to the Health Functional Food Cycle, the functionality of EPA (Eicosapentaenoic acid) and DHA has been shown to improve blood neutrality and blood circulation, and CLA has been shown to help reduce body fat in overweight adults. In addition, EPA and DHA have been reported to improve cognitive function, to inhibit cancer cells, and to have an anti-inflammatory effect. The lack of intake of EPA and DHA is associated with the development of fetal development, general cardiovascular disease and the risk of developing Alzheimer's disease . CLA has been reported to be effective in anti-cancer, cholesterol lowering and diabetes. VA (trans-vaccenic acid) is converted to CLA by 19% of the amount absorbed from the human body.

Healthy milk will be possible if the content of EPA, DHA, CLA, and VA is increased and the proportion of healthy fatty acids, omega-3 and omega-6, is changed to 1: 4 or less (Simopoulos, 2002 ). However, the products that have been modified to produce milk-derived milk fatty acid composition in Korea include DHA as milk, CLA as milk, omega-3 and omega-6 ratios of 1: 4 Has been sold, but the product that implements each product as a single product has not been sold yet.

On the other hand, as a domestic patent for a method for increasing the functional fatty acid content derived from milk from milk, Patent Document No. 1995-0006782 (1995.06.22) entitled "n-3 fatty acid-accumulated milk and dairy cat food composition" 1996-0014849 (October 21, 1996) "A method for producing a feed additive for producing milk having a high polyunsaturated fatty acid content", Registered Patent Publication 10-0431906 (2004.05.17) "And a water-soluble flaxseed oil-containing feed additive composition for producing milk containing omega-3 fatty acid, etc., and the like are disclosed in Patent Document 10-1187697 (Oct.

Patent Publication No. 1995-0006782 (June 22, 1995) contains fish meal and emulsifying adjuvant to increase omega-3 fatty acid content including DPA, EPA and DHA. Patent Publication No. 1996-0014849 (published on Oct. 21, 1996) discloses a method of mixing fish oil with kaolin at room temperature, granulating it by extrusion molding, coating a composite coating comprising jane, cellak, palmitic acid and propylene glycol in granular fish oil, It is a patent for the manufacturing method of additives. Patent Document 10-0431906 (Apr. 17, 2004) discloses a method of feeding a feed composition comprising a heat-treated cellulosic soybeans alone or a heat-treated cellulosic soybean and linseed oil mixture to a cow for a short period of time at a high temperature, It is reported that the CLA content in the examples increased to 11.85 ± 0.52 mg / g with the increased patent. Patent Application No. 10-1187697 (Oct. 20, 2012) discloses that omega-3 content is increased by feeding an additive mixture of flaxseed oil and green tea catechin extract to a cow.

Thus, the domestic patent also increases the contents of CLA, VA, DHA and EPA with one feed composition, and the ratio of omega-3 to 6 is not 1: 4.

Therefore, the present inventors have studied to develop a product which can increase the content of EPA, DHA and CLA, and can achieve the ratio of 1: 4 omega-3 and 6 at the same time.

Patent Publication No. 1995-0006782 Patent Publication No. 1996-0014849 Patent Registration No. 10-0431906 Patent Registration No. 10-1187697

Accordingly, an object of the present invention is to provide a milk having a healthy fatty acid in which the ratio of omega-3 to omega-6 is changed to 1: 4 while the content of EPA, DHA, CLA, And to develop a feed composition for production.

In order to achieve the above object, the present invention provides a dairy composition comprising a roughage, a by-product feed, a concentrated feed stuff, and an additive, wherein the total feed composition 2 to 4% by weight of flaxseed, canola and fish oil as fatty acid sources and 2 to 4% by weight of sorghum, millet and peas as excipient, wherein the functional fatty acid is VA (trans-vaccenic acid) The present invention relates to a dairy composition for producing milk having a fatty acid composition of 1: 1 to 1: 4 in the ratio of omega-3 to 6: CLA (conjugated linoleic acid), EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) .

In the present invention, the term " roughage " means a feed having a low energy content (plasticized nutrient content), such as grasses, hay, silage, corn, It is a feed for livestock, especially herbivores. In livestock with rumen, physiologically some amount should be maintained. In addition, root vegetables are treated as roughage because they have high water content, but they are treated as concentrated feeds when dried (Nutrition Dictionary, March 15, 1998, Academy Book).

The "by-product feed" mentioned above means a secondary product used as feed for various agricultural products or various kinds of bark (peeled bark) Rural Development Administration).

The term " concentrated feed stuff "as used herein includes cereals, beans, and calamari. This feed contains many plasticizers, but many of the ingredients are biased to one side, and many of them have different properties. Therefore, when mixing actual feeds, various kinds of concentrated feeds should be added. Concentrated feed is mainly used for livestock and cattle producing milk and eggs (Doosan Encyclopedia).

Typical formulated feeds are made up including such forage, by-product feed, concentrated feed and feed additives.

In the present invention, a functional feed composition having a novel composition ratio capable of containing various functional fatty acids and improving the ratio of omega 3, 6 was developed by containing a specific seed, fish oil, and excipient at a specific ratio in a general compound feed .

Generally, the amount of fatty acid in the feed of milk cows is about 4 ~ 5%, and the fat introduced into the rumen through the feed hydrolyzes the ester bond of triglyceride, phospholipid and glycolipid. Hydrolysis of the rumen is caused by lipolytic enzymes of rumen microbes, followed by in vivo curing of polyunsaturated fatty acids. Linolenic acid (LA) and α-linolenic acid (LNA) are produced by rumenic acid as an intermediate metabolite in the isomerization of double bond of cis-12 to trans-12 and the reduction of double bond of cis-9, rumenic acid is converted to VA and stearic acid is produced as a final product. Endogenous CLA synthesis also proceeds by the action of Δ9-desaturase.

The composition of the present invention can be used as a functional food by using specific seeds and fish oil as a fatty acid source and containing specific grains as an excipient and controlling its content. Milk derived from cows raised by feeding these feed compositions not only has higher functional fatty acid content but also can be adjusted to a balanced ratio of omega-3, 6 (1: 4 or less), satisfying the desire of consumers who desire healthy milk .

The feed composition for producing milk having enhanced functionality as described above is characterized by containing 2 to 4% by weight of flaxseed, canola and fish oil as a fatty acid supply source, 2 to 4% by weight of millet, millet and pea as an excipient do. In particular, the milk produced by feeding the feed composition of the present invention to cows has the advantage that it contains all of VA, CLA, EPA and DHA as functional fatty acids and has a fatty acid composition with a ratio of omega-3 to 6 of 1: 1 to 1: 4 have.

In a preferred embodiment of the present invention, the flaxseed, canola seed and fish oil contained in the feed composition may be 1.80, 0.90 and 0.15 wt%, respectively.

The sorghum, millet and pea may be 1.5, 1.35 and 0.3 wt%, respectively.

In another preferred embodiment of the invention, the overall feed ratio of the feed composition is 6-8% by weight oat, 6-8% by weight alfalfa, 6-8% by weight timothy, 4-6% by weight tall fescue, 5-7% 1 to 2% by weight of filaments, 1 to 3% by weight of flaxseed, 0.1 to 1.0% by weight of peas, 0.1 to 2.0% by weight of canola seeds, 0.05 to 0.2% of fish oil 15-25% by weight of TMR cone mix, 5-15% by weight of bromide, and 20-30% by weight of water.

According to the embodiment of the present invention, as a result of feeding the feed composition having the characteristic content ratio and checking the functional fatty acid content ratio, the crude oil obtained from the cow fed with a sufficient amount of the functional food for 6 weeks contained VA, CLA, EPA and DHA were all highly contained. Especially, the ratio of omega-3 and 6 was 1: 3.9, and the best fatty acid analysis results were obtained (see Example 2 and Table 5). Unlike milk, which contains only specific fatty acid contents such as CLA and DHA, it has a remarkable effect in that it contains all the functional fatty acids. At the same time, it has the advantage of maintaining the ratio of healthy omega 3 and 6 , And can be used for the production of milk having excellent functionality, palatability and merchantability.

As described above, crude oil milked in the cow fed with the feed composition of the present invention increased the contents of EPA, DHA, CLA and VA and increased the ratio of 1: 4 omega-3 and omega-6, which are healthy fatty acid compositions . In the case of feeding large amounts of unsaturated fatty acids and CLA precursors to cows, microbes in the rumen of cows tend to convert unsaturated fatty acids into saturated fatty acids, which leads to an increase in saturated fatty acids in milk. Increased feed intake led to improved EPA, DHA, CLA, VA and omega-3 composition It can be used to produce milk.

Hereinafter, the present invention will be described in detail with reference to examples. However, these examples are intended to further illustrate the present invention, and the scope of the present invention is not limited to these examples.

Example 1: Preparation of functional feeds

The inventors of the present invention have studied a feed composition capable of producing milk with improved fatty acid content and omega-3 and 6 ratio, and then devised a functional feed composition containing seeds with high oil content, fish oil and excipients. In the present invention, flaxseed and canola seeds were used as oil-rich seeds, and sorghum, millet, and pea were used as excipients. Table 1 below shows an example of the blending ratio of common fodder fodder mixed with flaxseed, canola seed, fish oil, and excipient.

The raw materials according to Table 1 were purchased from importers and Seoul Milk designated companies. Mixed feeds (cone mixes and mixes) were purchased from a compound feed factory designated by Seoul Milk and used. The fish oil used in the test was Canadian acid mixed with sardine oil, salmon oil and cod liver oil. The raw materials were weighed according to the mixing ratio shown in the following Table 1 according to the manufacturing process chart shown in Table 2, and then mixed for 30 minutes in a blender to prepare a test feed.

Figure pat00001

Figure pat00002

In Table 2, the supplementary feeds were supplied to the microorganisms, and the forage means long-grain feedstuffs in which the particles of the two parts, the camphor and the candle parts are long. These concentrated feeds are processed as bulk feedstocks for use in compound feed mills and include pellets or crumbled feed. The fermented feed is composed of a raw material containing a large amount of water. The liquid raw materials were controlled according to quality standards such as water, molasses and other raw materials.

Table 3 shows the general components of the compound feed (TMR) prepared at the above mixing ratio.

Figure pat00003

NDF, neutral detergent fiber: neutral detergent insoluble fiber

ADF, acid detergent fiber: acid detergent insoluble fiber

Example  2: Oil quality and flow rate changes according to feeding rate

The TMR functional feed prepared in Example 1 was fed to the two test ranches (A, B) in an amount that could be fed to the cows for 5 days. Changes in oil quality and flow rate of the ranch fed with the test diets were confirmed for 7 weeks. The results are shown in Table 4 and Table 5, respectively.

Figure pat00004

Figure pat00005

As shown in Tables 4 and 5, when the compounded diets of the present invention were fed, the milk content was slightly decreased, but the protein, somatic cell count, bacterial count, and cow milk flow rate were not affected.

Example 3: Functional fatty acid composition change according to feeding amount of feed

After feeding the functional feeds in the same manner as in Example 2, changes in the fatty acid composition of each feeding period were analyzed in the test ranches A and B, and the results are shown in Tables 6 and 7, respectively.

Figure pat00006

VA, trans vaccenic acid; LA, linoleic acid; LNA, alpha-linolenic acid; AA, arachidonic acid; W3, omega-3 fatty acid; W6, omega-6 fatty acid

Figure pat00007

VA, trans vaccenic acid; LA, linoleic acid; LNA, alpha-linolenic acid; AA, arachidonic acid; W3, omega-3 fatty acid; W6, omega-6 fatty acid

As shown in Tables 6 and 7, in the 6th week after feeding 1.8 kg of the functional food, VA content 15.2 mg / 100 mL, CLA content 20.6 mg / 100 mL, EPA content 1.49 mg / 100 mL, DHA , 1.42 mg / 100 mL, and an omega-3: 6 ratio of 1: 3.9.

In the 6th week after the feeding of 1.8kg of the functional diet, the experimental ranch (B) also had a VA content of 15.1 mg / 100 mL, CLA content of 21.3 mg / 100 mL, EPA content of 1.12 mg / 100 mL, DHA content of 1.3 mg / 100 mL, : 6 ratio of 1: 5.2. When the feed of functional diets was further increased, the ratio of omega-3 to omega-6 was positive but the functional fatty acid content was decreased.

Comparative Example: Fatty Acid Analysis of Commercial Milk

Table 8 below shows the fatty acid analysis results for various milk products currently available in Korea. In Table 8 below, A represents SPC Milk Plus, B represents daily milk-lower organic milk, C: Namyang Dairy Einstein, and D: Seoul Milk general milk.

Figure pat00008

VA, trans vaccenic acid; LA, linoleic acid; LNA, alpha-linolenic acid; AA, arachidonic acid; W3, omega-3 fatty acid; W6, omega-6 fatty acid

In Table 8, product A is milk with increased CLA content through feed feeding, product B is organic milk, product C is feed-derived DHA milk, and product D is general milk.

As shown in Table 8, product A had a high CLA content and a ratio of omega-3 to 6 of 1: 3.7, but EPA and DHA were not detected. Organic milk product B had higher CLA content than normal milk, but EPA and DHA were not detected. The ratio of omega-3 to 6 was 1: 7.6, product C milk had a DHA content of 2.2 mg / 100 mL, CLA was 14.6 mg / , The ratio of omega-3 to 6 was 1: 6.8, that of product D was 12.4 mg / 100 mL of CLA, and the ratio of omega-3 to 6 was 1: 10.7.

Therefore, it was confirmed that conventional commercial milk contains not only VA, CLA, EPA and DHA but also OMEGA 3 and 6 at a ratio of 1: 4.

On the other hand, the milk obtained by feeding the functional diet developed in the present invention contains all of VA, CLA, EPA and DHA and improves the ratio of omega-3 and 6, thereby enabling milk production with a healthy functional fatty acid composition.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (4)

In a dairy composition comprising a roughage, a by-product feed, a concentrated feed stuff, and an additive,
Characterized in that it comprises 2 to 4% by weight of flaxseed, canola and fish oil as a fatty acid source for the whole feed composition and 2 to 4% by weight of sorghum, millet and pea as excipient, and as a functional fatty acid VA (trans-vaccenic to produce milk having a fatty acid composition of 1: 1 to 1: 4 in the ratio of omega-3 to 6, including all of acid, CLA (conjugated linoleic acid), EPA (Eicosapentaenoic acid) and DHA (docosahexaenoic acid) Dairy cattle composition.
The composition of claim 1, wherein the flaxseed, canola seed and fish oil are included at 1.80, 0.90 and 0.15 wt%, respectively.
2. The composition of claim 1, wherein said sorghum, millet and pea are included at 1.5, 1.35 and 0.3 wt%, respectively.
The composition of claim 1, wherein the composition comprises 6-8 wt% oat, 6-8 wt% alfalfa, 6-8 wt% timothy, 4-6 wt% tall fescue, 5-7 wt% beet pulp, 1-2% by weight, 1-2% by weight of flaxseed, 1-3% by weight of flaxseed, 0.1-1.0% by weight of peas, 0.1-2.0% by weight of canola seeds, 0.05-0.2% by weight of fish oil, 25% by weight, 5-15% by weight of bromide, and 20-30% by weight of water.

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR950006782A (en) 1993-08-17 1995-03-21 정규형 Compact Disc Auto Changer Player
KR960014849A (en) 1994-10-21 1996-05-22 Open showcase with prefabricated cold storage
KR100431906B1 (en) 2001-10-16 2004-05-17 주식회사 애그리브랜드 퓨리나코리아 Feed increasing CLA content in the milk and manufacturing method thereof
KR101187697B1 (en) 2009-03-02 2012-10-05 안남순 annimal food comprising omega-3 fatic acid

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR950006782A (en) 1993-08-17 1995-03-21 정규형 Compact Disc Auto Changer Player
KR960014849A (en) 1994-10-21 1996-05-22 Open showcase with prefabricated cold storage
KR100431906B1 (en) 2001-10-16 2004-05-17 주식회사 애그리브랜드 퓨리나코리아 Feed increasing CLA content in the milk and manufacturing method thereof
KR101187697B1 (en) 2009-03-02 2012-10-05 안남순 annimal food comprising omega-3 fatic acid

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