KR20070059503A - Burkholderia sp. ppk003 strain and methods of promoting turfgrass growth and preventing turfgrass disease by using it - Google Patents

Abstract

A microorganism Burkholderia sp. PPK003 and methods for promoting turfgrass growth and preventing turfgrass disease by using the same microorganism are provided to promote growth of turfgrass and inhibit growth of plant pathogenic bacteria causing turfgrass disease simultaneously, so that quality and production efficiency of the turfgrass are improved. The microorganism Burkholderia sp. PPK003(KCCM 10712P) isolated from the soil having turfgrass is provided. The microbial agent for promoting turfgrass growth and preventing turfgrass disease contains Burkholderia sp. PPK003(KCCM 10712P). The turfgrass growth is promoted and turfgrass disease is prevented by spraying Burkholderia sp. PPK003(KCCM 10712P) or the microbial agent containing Burkholderia sp. PPK003(KCCM 10712P) into the turfgrass or the soil with turfgrass.

Description

Burkholderia spp. 003 strain and method for inhibiting turf growth and turf disease development using the same {Burkholderia sp. PPK003 strain and Methods of Promoting Turfgrass Growth and Preventing Turfgrass Disease by using it}

1 is a photograph showing growth growth of grass by PPK003. A: control, B: positive control, C: PPK003 treatment pictures were taken after the end of the experiment.

Figure 2 shows a growth promoting picture of the grass by PPK003. Control and positive control KLP, microbial preparation pinefield was compared. Compared to that of the control, the growth of the grassy ground was significantly promoted in the PPK003, and the root length was also greatly promoted.

3 shows that PPK003 has antagonistic activity against grass pathogens. A: Antagonist against grass-spot pathogen, B: Antagonist against large-patch pathogen, C: Antagonist against brown-patch pathogen.

The present invention relates to a novel Burkholderia strain that is purely isolated from soil planted with grass and its use, and more particularly Burkholderia sp. PPK003 (Accession No .: KCCM 10712P), relates to a method for inhibiting grass pathogens using the microorganisms, and a method for promoting grass growth using the microorganisms.

Existing chemical pesticides not only cause severe environmental pollution, but also concentrate along the food chain of the ecosystem, which seriously affects human health. In addition, due to the increased resistance of microorganisms to conventional chemical pesticides, there is a problem that the use of a larger amount of pesticides. Therefore, reducing environmental pollution, the development of pollution-free microbial pesticides are urgently required.

Some types and characteristics of microorganisms useful for agriculture are as follows. First of all, lactic acid bacteria grow very rapidly at 20-40 ℃ with or without air and produce lactic acid during the culture process, lower acidity (pH 4) and produce hydrogen peroxide (H ₂ O ₂ ) to inhibit the growth of pathogenic microorganisms. It is known to prevent soil pests. In addition, by producing various physiologically active substances (vitamins, amino acids, nucleic acids, etc.), antibacterial substances, and anticancer substances, crops increase the self-defense ability of plants. It turns out. In addition, yeast is a major microorganism of heat generation during fermentation of agricultural by-products such as rice bran and bran, and has excellent ability to decompose organic matter and shows fermentability and viability under various conditions. Therefore, it is known to exert an odor removal effect when spraying on odor generating sources (barn floor, etc.). Actinomycetes are beneficial microorganisms that grow in soil and produce antibiotics that kill pathogens or stop their growth as natural enemies of pathogenic fungi. Activated Soil of High Quality Soil containing lots of organic material has a high density of actinomycetes. Soil containing high organic matter during fermentation has a high density of actinomycetes. When the compost fermentation temperature is 70 ~ 80 ℃, the ability to control the pests of soil in the compost is due to the large amount of antibiotics produced by actinomycetes. In addition, the unique smell of soil is the smell of actinomycetes. The reason for sterilizing the mushroom medium after fermentation is also to cultivate actinomycetes, and it is also widely known that the well-preserved actinomycetes have almost no contamination of various germs during the sticking process after inoculation. In addition, a fungus called Mycorrhiza grows in close contact with crop roots, absorbs poorly soluble components in the soil (especially phosphoric acid) and provides them to the crop, and also acts as a physical barrier on the root surface to prevent root diseases. have. In addition, there is a photosynthetic bacterium that performs photosynthesis using sunlight as if it is a plant. The bacterium has a crimson color, has a high protein content in the cells, and is known to produce various vitamins, minerals, bioactive substances and antibacterial and antiviral substances. It grows while ingesting various odor generating substances such as ammonia, hydrogen sulfide, and organic acids, and may exhibit an odor removal effect. Photosynthetic bacteria also play a role in gas removal to prevent gas damage due to the use of immature oil in the house, and also has the ability to purify contaminated dirty water, which can be used as a microorganism for treatment of fish farms. Widely used as a microbial insecticide, B / T bacteria produce toxins in the cells, and when the larvae of pests (blue worms, beetroot moths) eat this toxin, the intestines are destroyed and die. Recently, B / T bacteria have been developed that kill soil harmful nematodes.

In recent years, efforts have been made to change the microbial phase in soil in order to benefit plant growth and human health. Bacterization refers to the inoculation of microbial cultures into crop growth or growth, which sometimes leads to significant crop yields, but it is still unclear what role the inoculated microbes play in the root zone. Not. Some microorganisms cannot survive in the root zone by being rejected by the original existing root zone microorganisms, as long as there is a kind that maintains its density by stably surviving and growing in the root zone within the crop growth period. At this time, the microorganisms that stably settle and proliferate at the root surface are called 'root zone microorganisms'.

The rooting settlement of microorganisms is a very active process, in which the microorganisms survive on the seed surface or in the soil and grow using carbohydrate and amino acid-rich seed secretions and continue to grow along the roots attached and growing on the root surface. . In addition, the rhizosphere microbes are passively moved to the lower part of the root by watering.

Representative rhizosphere microorganisms include Pseudomonas, azotobacter, and Bacillus. 'Is called. In the past, the beneficial effects of plant-derived root-root microorganisms were only recognized for root crops such as radish, potatoes and sugar cane, but recently, the positive effects have been recognized in various crops such as oats, beans, cotton, peanuts, balsam, rice and vegetables. .

Growth promotion and pest control effects by microbial inoculation should be recognized on both sides of the coin. That is, many researchers have revealed that the microorganisms having a growth promoting effect have a pest suppression effect, and that the microorganisms with a disease inhibiting function promote plant growth. For example, some microorganisms (Pseudomonas bacteria) that have been shown to promote plant growth have been shown to promote relative growth of crops by reducing the density of harmful bacteria and fungi in the rhizosphere.

That is, most of the plant growth promoting root zone microorganisms known so far have been known to promote crop growth indirectly by controlling harmful root zone microorganisms.

On the other hand, rhizosphere microorganisms sometimes produce a substance (physiologically active substance) that promotes plant growth, thereby directly promoting plant growth. That is, these bioactive substances are known to exert effects such as promoting root hair growth, promoting root and stem growth by promoting nutrient absorption by the root. Eventually, plant growth-promoting root-strain microorganisms, in addition to the growth promoting effect by pest control, produce special physiologically active substances to change crop physiology to double the plant's self-defense ability against invading germs, thereby preventing disease and promoting growth. .

Biological control mechanisms by rhizosphere microorganisms are known to be due to antimicrobial activity, competition, lysis, specific nutrient depletion, cyan production, and the like.

In the case of antimicrobial activity, rhizosphere microorganisms in which antimicrobial activity (antagonism) against pathogens is stabilized in the laboratory do not necessarily exhibit the same antimicrobial activity in crop cultivation sites, but in many cases, there is a deep correlation.

Antibacterial activity is known to be mainly caused by antibiotics and siderophore secreted by rhizosphere microorganisms, but the main effect of pest biocontrol is mainly due to antibiotics. For example, some plant growth-promoting root zone microorganisms secrete a powerful antibiotic called phenazine around the roots of wheat when administered to wheat cultivated soil, thereby suppressing pathogens and increasing yields.

Competition means the effect of inhibiting pathogens by incapacitating pathogens by competition between root-strain microorganisms and pathogens against the root-sensitive nutrients and root-sensitive areas of the roots. Pseudomonas microorganisms (Pseudomonas bacteria) promote plant growth by rapidly ingesting various nutrients and depleting essential nutrients for use by pathogens. By preoccupying the site, pathogens are prevented from invading the roots, thereby preventing the occurrence of disease.

Lycobacterium refers to the mission of pathogenic fungi by the action of fungal destructive enzymes produced by plant-promoting rhizosphere microorganisms. In other words, plant growth-promoting root zone microorganisms produce a fungal cell wall lytic enzyme called chitinase to destroy the cells of hospital fungi (pisium, etc.), thereby preventing and increasing disease effects.

Iron, one of the trace elements, is an essential element of microbial growth, but iron is insoluble in trivalent iron and cannot be directly used for microorganisms. Microorganisms can utilize iron by producing a substance called ciderofo in order to absorb and utilize these insoluble iron and making a chelate compound having a cederopo-iron. Promote plant growth By producing a large amount or excellent function of side-rope microorganisms, iron is rapidly used to deplete the iron components necessary for the growth of harmful microorganisms (including pathogens) in the root zone. You will not be able to break into roots.

Many rhizospheres produce cyanide compounds, which contribute to pathogen control. In other words, the cyan compound can inhibit the growth by causing fatal damage to the pathogens of the root zone.

Certain microorganisms have the ability to antagonize plant pathogenic fungi. Occupying the surface of the plant in advance so that the pathogen is inaccessible, or by first ingesting the nutrients essential for growth may inhibit the growth of the pathogen. The antifungal microorganisms reported so far are known to be derived from bacteria such as Pseudomonas, Bacillus, Streptomyces, Agrobacterium, Burkholderia , and fungi such as Trichoderma, Gilocladium, and Fusarium . However, few microorganisms promote the growth of grass and simultaneously control the grass disease.

Therefore, the inventors of Burkholderia sp. It was found that PPK003 (Accession No .: KCTC 10712P) antagonistically acts as a causative agent of grass disease, large patches, brown patches, grass coin blight, and promotes the growth of grass.

Accordingly, the present invention has been made to solve the above problems, and an object of the present invention is to provide a microbial agent comprising the same, and a strain for inhibiting grass growth and grass pathogens extracted from soil microorganisms.

In addition, another object of the present invention is to provide a cultivation method that can improve the marketability and production efficiency of grass using the strain or microbial agent comprising the same.

The invention microbial new strain Burkholderia sp. PPK003 (Accession Number: KCCM 10712P) is provided. Burkholderia sp. PPK003 was purely isolated from the soil where grass was planted, and identified as the new strain PPK003 ( Burkholderia sp) by analyzing the gene sequence, and has excellent antifungal activity and plant growth promoting activity.

The present invention provides a microbial agent comprising the microorganism or a metabolite thereof. Microbial formulations are used in the form of liquids, powders, alginate prills, etc.Then, the effect of the product by spraying by mixing the pete as a filler or the adhering agent as a filler. There was an attempt to increase it.

In recent years, spores are immobilized in biopolymers such as alginic acid, polyacrylamide, and carrageenan, and then mixed with various additives (nutrients, electrodeposited UV blocking agents, enhancers, PGPR, etc.) and sprayed onto packages.

The present invention provides a method for suppressing the development of turf disease using the microorganisms.

Burkholderia sp. PPK003 (Accession No .: KCCM 10712P) Provides a method of inhibiting the pathogenesis of grass by spraying microorganisms alone or in combination with other microorganisms, or spraying live bacteria directly in the form of a microbial agent containing other additives.

The present invention provides a method for promoting grass growth using the microorganism.

Burkholderia sp. PPK003 (Accession No .: KCCM 10712P) Provides a method of promoting the growth of grass by spraying microorganisms directly or by mixing them with other microorganisms, or by spraying them in the soil in the form of microbial agents containing other additives. The additives include nutrients (compost, etc.), electrodeposition agents, and the like, for promoting plant growth.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the following examples are merely to illustrate the present invention, but the content of the present invention is not limited to the following examples.

Example 1 Isolation Identification and Characteristics of Strains

The new strain PPK003 ( Burkholderia sp) was isolated purely from the soil where grass was planted. Microorganisms having excellent efficacy were isolated and identified as a new strain PPK003 ( Burkholderia sp) , deposited with the Korea Microorganism Conservation Center, and given accession number KCCM 10712P.

Characteristics of the strain of the new strain PPK003 ( Burkholderia sp)

1. Cultural features

Cultured in LB (10 g of tryptone, 5 g of yeast extract powder, 10 g of sodium chloride, 15 g of agar, 1 l of distilled water), and cultured at 25-30 ° C.

2. Morphological features

PPK003 ( Burkholderia sp) becomes yellow until the first 15 hours of plate culture, but gradually forms fluorescent rounded colonies, and the culture solution becomes yellow fluorescent color even during shaking culture.

< Example 2> Grass growth promotion by the new strain PPK003 ( Burkholderia sp)

(1) Method of implementation

Pots are made using bent glass made from sterilized soil (a ratio of sand to peat moss: 1: 9) and a hole-cutter with a diameter of 11 cm. After that, cut the grass short, and then microbial treatment. After adjusting the OD value of the microorganism to 0.5 and treating a total of 20 ml three times a week, the difference in turfgrass growth of one week is observed.

In order to know the results of root growth promotion, the control group, the positive control KLP, the microbial preparation pinefield, and the experimental group PPK003 were treated.

 (2) results

The growth difference between the treatment group treated with PPK003 ( Burkholderia sp) , KLP which is known to have an effect of promoting turfgrass activity, and the control group treated with nothing was found (see FIG. 1).

Especially PPK003 ( Burkholderia sp ) It was found that the effect of promoting grass growth was superior to that of KLP.

It was also found that the growth of the grassy ground was significantly promoted, and the root length was also significantly increased, compared to that of the control, which had little growth (see FIG. 2).

In addition, as a result of measuring the chlorophyll content, it was found that the color of the clofil was better than that of the control.

The results of this experiment suggest the possibility of facilitating the growth of the grass, making it easier to manage the turf, which can be a little tricky. Moreover, given that the use of the grass has many aesthetic roles, the grass is of higher quality. It was found that I can keep the state of.

Example 3 Antagonist Against Grass Pathogens of PPK003 ( Burkholderia sp)

(1) Conduct method

New strain PPK003 ( Burkholderia sp ) Three pathogens and microorganisms were tested in PDA medium (potato extract 300 g, glucose 20 g) to test their antagonism against yellow-patch, brown-patch and grass-spot. 15 g of agar and 1 l of distilled water were incubated incubated. At this time, KLP strains already known as antagonism against pathogens were used as a positive control.

(2) results

When observed after 24 hours of culture, it was found that the mycelia of pathogens did not grow in the area where the PPK003 ( Burkholderia sp) was grown (see FIG. 3).

This means that the microorganisms dominate the pathogens, and where the microorganisms grow first, the grass pathogens would not grow. This may be an effective way to prevent turf disease. In other words, if the microorganisms are regularly treated before the occurrence of turf disease, and the microorganisms are always present in the soil, the turf disease may be prevented.

The new strain PPK003 ( Burkholderia sp ) according to the present invention promoted the growth of grass. In addition, when these microorganisms were replaced with grass pathogens, they were found to have antagonism against the pathogens. By using this microorganism, not only the quality of the turf can be improved, but also the turf can be easily managed and the turf disease can be prevented.

Claims (4)

Microbial new strain Berkholdereria spp. PPK003 (Accession No .: KCCM 10712P) that promotes turf growth and inhibits turf disease. A microbial preparation comprising the microorganism according to claim 1 or a metabolite thereof. Method of inhibiting the development of turf disease using the microorganism according to claim 1. A method for promoting turf growth using the microorganism according to claim 1.

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