KR20060089391A - 돼지 인플루엔자 바이러스ａ／ｓｗｉｎｅ／ｋｏｒｅａ／ｓ109／2004(ｈ9ｎ2) 및이의 백신 - Google Patents

돼지 인플루엔자 바이러스ａ／ｓｗｉｎｅ／ｋｏｒｅａ／ｓ109／2004(ｈ9ｎ2) 및이의 백신 Download PDF

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KR20060089391A
KR20060089391A KR1020050010416A KR20050010416A KR20060089391A KR 20060089391 A KR20060089391 A KR 20060089391A KR 1020050010416 A KR1020050010416 A KR 1020050010416A KR 20050010416 A KR20050010416 A KR 20050010416A KR 20060089391 A KR20060089391 A KR 20060089391A
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윤인중
서상희
김현수
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주식회사 중앙백신연구소
주식회사 백신리더
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Abstract

본 발명은 돼지 인플루엔자 바이러스 A/Swine/Korea/S109/2004(H9N2)및 이의 백신에 관한 것이다. 특히 본 발명은 조류 인플루엔자 바이러스와 사람 인플루엔자 바이러스간의 조합으로 생성된 신규 인플루엔자 바이러스 A/Swine/Korea/S109/2004(H9N2) 및 이의 백신을 제공한다. 본 발명에 따라 돼지 인플루엔자 바이러스에 대한 면역을 효과적으로 형성시킬 수 있으며 따라서 돼지 농장에서의 인플루엔자 바이러스에 의한 질환 발병을 현저히 감소시켜 경제적인 손실을 방지할 수 있다.
인플루엔자 바이러스, 돼지, 백신

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돼지 인플루엔자 바이러스 Ａ／ＳＷＩＮＥ／ＫＯＲＥＡ／Ｓ109／2004(Ｈ9Ｎ2) 및 이의 백신{A/Swine/Korea/S109/2004(H9N2) INFLUENZA VIRUS AND VACCINE THEREFOR}
도 1은 A/Swine/Korea/S109/2004(H9N2), A/Swine/Korea/S81/2004(H9N2) 및 A/Swine/Korea/S83/2004(H9N2) 인플루엔자 바이러스의 계통발생학적 관련성을 HA(Haemagglutinin) 유전자 염기서열로 분석한 것이다.
도 2는 A/Swine/Korea/S10/2004(H1N1) 인플루엔자 바이러스의 계통발생학적 관련성을 HA(Haemagglutinin) 유전자 염기서열로 분석한 것이다.
도 3은 A/Swine/Korea/S10/2004(H1N1), A/Swine/Korea/S109/2004(H9N2), A/Swine/Korea/S81/2004(H9N2) 및 A/Swine/Korea/S83/2004(H9N2) 인플루엔자 바이러스의 계통발생학적 관련성을 M(Matrix) 유전자 염기서열로 분석한 것이다.
도 4는 A/Swine/Korea/S10/2004(H1N1), A/Swine/Korea/S109/2004 (H9N2) 및 A/Swine/Korea/S452/2004(H9N2)의 항바이러스제인 아만타딘에 대한 비감수성을 나타낸 것이다.
본 발명은 인플루엔자 바이러스 A/Swine/Korea/S109/2004(H9N2) 및 이의 백신에 관한 것이다.
20세기에 세계적으로 3종의 유행병이 발병하였었다(J. K. Taubenberger et al., Science 275, 1793 (1997) ; Schafer, J. R. et al., J. Virol. 194, 781 (1993) ; C. Scholtissek, W. Rohde, V. Von Hoyningen, R. Rott, Virology 87, 20 (1978) ; Y. Kawaoka, S. Krauss, R. G. Webster, J. Virol. 63, 4603 (1989)). 조류 인플루엔자계 H1N1 바이러스는 1918년에 "스페인 독감"을 야기하였고, 재조합 H2N2 인플루엔자 바이러스와 H3N2 인플루엔자 바이러스는 1957년 "아시아 독감'과 1968년 "홍콩 독감"을 발병시켰다. 재조합 H2N2 유행성 인플루엔자 바이러스와 H3N2 유행성 인플루엔자 바이러스는 사람과 조류 인플루엔자 바이러스간의 조합에 의하여 돼지에서 형성된 것으로 추정된다.
서브타입 인플루엔자 A형 바이러스는 15종의 HA(haemagglutinin)와 9종의 NA(neuraminidase)로 이루어져 있으며(Webster, R. G. et al., Microbiol. Rev. 56, 179 (1992)), 이중 H9N2 및 H5N1 서브타입은 사람 인플루엔자 유행병을 야기할 수 있는 가장 큰 가능성을 가지고 있다. H5N1 인플루엔자 바이러스는 중국, 베트남 및 타이완을 포함한 많은 아시아 국가에서 확인되는 고유종이며(S. H. Seo, E. Hoffmann, R. G. Webster Nat. Med. 8, 950 (2002) ; T. H. Tran, et al., N. Engl .J. Med. 350,1179 (2004) ; K. S. Li, et al., Nature 430, 209 (2004)), H9N2 인플루엔자 바이러스는 한국 및 중국과 같은 다수의 국가에 만연해있는 실정이다. 1999년 홍콩에서는 2명의 어린이가 H9N2 인플루엔자 바이러스에 감염된 바 있다(M. Peiris, et al., Lancet 354, 916 (1999)).
한편, 돼지는 유행성 인플루엔자 바이러스를 창조하여 사람에서 사람으로 전이시키는 "혼성관"으로 여겨진다(C. Scholtissek, Med. Princ. Pract. 2, 65 (1990)). 즉 돼지는 조류 인플루엔자 바이러스와 사람 인플루엔자 바이러스 모두에 감염가능하며(H. Kida, et al., J. Gen. Virol. 75, 2183 (1994)), 이들 바이러스간의 조합으로 새로운 형태의 인플루엔자 바이러스가 제조되기도 한다.
상기 종래기술의 문제점을 해결하기 위하여 안출된 것으로서, 본 발명은 조류 인플루엔자 바이러스와 사람 인플루엔자 바이러스 간의 재조합으로 생성된 신규한 돼지 인플루엔자 바이러스를 제공하는 것을 목적으로 한다.
또한 본 발명은 조류 인플루엔자 바이러스와 사람 인플루엔자 바이러스 간의 재조합으로 생성된 신규한 돼지 인플루엔자 바이러스에 대해 면역을 형성시킬 수 있는 인플루엔자 백신 조성물을 제공하는 것을 목적으로 한다.
본 발명은 사람 인플루엔자 바이러스와 조류 인플루엔자 바이러스 간의 조합으로 생성된 신규 돼지 인플루엔자 바이러스 및 상기 바이러스에 대한 백신 조성물에 관한 것이다.
본 발명의 돼지 인플루엔자 바이러스는, A/Swine/Korea/S10/2004(H1N1), A/Swine/Korea/S109/2004(H9N2), A/Swine/Korea/S81/2004(H9N2) 및 A/Swine/Korea/S83/2004(H9N2)이다.
상기 A/Swine/Korea/S10/2004(H1N1) 인플루엔자 바이러스는 PB2(Polymerase basic protein 2) 유전자를 제외한 나머지 유전자들, PB1, PA(Polymerase), HA(Hemagglutinin), NP(nucleoprotein), NA(Neuraminidase), M(matrix) 및 NS (nonstructural) 유전자는 사람 인플루엔자 바이러스 A/WSN/33(H1N1)와 유사하고, PB2 유전자는 조류 H9N2 인플루엔자 바이러스로부터 유래된 것이다. A/Swine/Korea/S10/2004(H1N1) 인플루엔자 바이러스는 2005년 1월 7일자로 대한민국 대전시 유성구에 소재한 한국생명공학연구원에 KCTC 10758BP로 기탁하였다.
A/Swine/Kores/S10/2004(H1N1) 인플루엔자 바이러스의 PB2 유전자는 서열번호 1에 기재된 염기서열을 포함하며, PB1 유전자는 서열번호 2에 기재된 염기서열을 포함하며, PA(Polymerase) 유전자는 서열번호 3에 기재된 염기서열을 포함하며, HA 유전자는 서열번호 4에 기재된 염기서열을 포함하며, NP 유전자는 서열번호 5에 기재된 염기서열을 포함하며, NA 유전자는 서열번호 6에 기재된 염기서열을 포함하며, M(matrix) 염기서열은 서열번호 7에 기재된 염기서열을 포함하며, NS 유전자는 서열번호 8에 기재된 염기서열을 포함한다. 또한 PB2 단백질은 서열번호 33에 기재된 아미노산 서열을 포함하며, PB1 단백질은 서열번호 34에 기재된 아미노산 서열을 포함하며, PA 단백질은 서열번호 35에 기재된 아미노산 서열을 포함하며, HA 단백질은 서열번호 36에 기재된 아미노산 서열을 포함하며, NP 단백질은 서열번호 37에 기재된 아미노산 서열을 포함하며, NA 단백질은 서열번호 38에 기재된 아미노산 서열을 포함하며, M 단백질은 서열번호 39에 기재된 아미노산 서열을 포함하며, NS 단백질은 서열번호 40에 기재된 아미노산 서열을 포함한다.
상기 A/Swine/Korea/S109/2004(H9N2) 인플루엔자 바이러스는 사람 인플루엔자 바이러스와 조류 인플루엔자 바이러스 간의 조합으로 이루어진 H9N2 혈청형의 바이러스로, 이의 PB2 유전자, HA 유전자 및 NA 유전자는 조류 인플루엔자 바이러스로부터 유래된 것이며, PB1 유전자, PA 유전자, NP 유전자, M 유전자 및 NS 유전자는 사람 인플루엔자 바이러스로부터 유래된 것이다. A/Swine/Korea/S109/2004(H9N2) 인플루엔자 바이러스의 PB2 유전자, PB1 유전자, PA 유전자, HA 유전자, NP 유전자, NA 유전자, M 유전자 및 NS 유전자는 각각 서열번호 9 내지 16에 기재된 염기서열을 포함하며, 이들의 단백질은 각각 서열번호 41 내지 48에 기재된 아미노산 서열을 포함한다. A/Swine/Korea/S109/2004(H9N2) 인플루엔자 바이러스는 2005년 1월 7일자로 대한민국 대전시 유성구에 소재한 한국생명공학연구원에 KCTC 10761BP로 기탁하였다.
상기 A/Swine/Korea/S81/2004(H9N2) 인플루엔자 바이러스는 사람 인플루엔자 바이러스와 조류 인플루엔자 바이러스 간의 조합으로 이루어진 H9N2 혈청형의 바이러스로, 이의 PB2 유전자, HA 유전자, NA 유전자 및 NS 유전자는 조류 인플루엔자 바이러스로부터 유래된 것이며, PB1 유전자, PA 유전자, NP 유전자 및 M 유전자는 사람 인플루엔자 바이러스로부터 유래된 것이다. A/Swine/Korea/S81/2004(H9N2) 인플루엔자 바이러스의 PB2 유전자, PB1 유전자, PA 유전자, HA 유전자, NP 유전자, NA 유전자, M 유전자 및 NS 유전자는 각각 서열번호 17 내지 24에 기재된 염기서열을 포함하며, 이들의 단백질은 각각 서열번호 49 내지 56에 기재된 아미노산 서열을 포함한다. A/Swine/Korea/S81/2004(H9N2) 인플루엔자 바이러스는 2005년 1 월 7일자로 대한민국 대전시 유성구에 소재한 한국생명공학연구원에 KCTC 10759BP로 기탁하였다.
상기 A/Swine/Korea/S83/2004(H9N2) 인플루엔자 바이러스는 사람 인플루엔자 바이러스와 조류 인플루엔자 바이러스 간의 조합으로 이루어진 H9N2 혈청형의 바이러스로, 이의 PB2 유전자, HA 유전자, NA 유전자, M 유전자 및 NS 유전자는 조류 인플루엔자 바이러스로부터 유래된 것이며, PB1 유전자, PA 유전자 및 NP 유전자는 사람 인플루엔자 바이러스로부터 유래된 것이다. A/Swine/Korea/S83/2004(H9N2) 인플루엔자 바이러스의 PB2 유전자, PB1 유전자, PA 유전자, HA 유전자, NP 유전자, NA 유전자, M 유전자 및 NS 유전자는 각각 서열번호 25 내지 32에 기재된 염기서열을 포함하며, 이들의 단백질은 각각 서열번호 57 내지 64에 기재된 아미노산 서열을 포함한다. A/Swine/Korea/S83/2004(H9N2) 인플루엔자 바이러스는 2005년 1월 7일자로 대한민국 대전시 유성구에 소재한 한국생명공학연구원에 KCTC 10760BP로 기탁하였다.
본 발명에 따른 돼지 인플루엔자 바이러스는 한국 돼지의 비강에서 직접 분리한 것으로, 이들의 바이러스들은 도 1 내지 3으로 도시한 계통발생학적 연관성을 갖는다. 도 1은 A/Swine/Korea/S109/2004(H9N2), A/Swine/Korea/S81/2004(H9N2) 및 A/Swine/Korea/S83/2004(H9N2) 인플루엔자 바이러스의 계통발생학적 관련성을 HA를 이용하여 분석한 것이고, 도 2는 A/Swine/Korea/S10/2004(H1N1) 인플루엔자 바이러스의 계통발생학적 관련성을 HA로 분석한 것이고, 도 3은 A/Swine/Korea/S10/2004(H1N1), A/Swine/Korea/S109/2004(H9N2), A/Swine/Korea/S81/2004(H9N2) 및 A/Swine/Korea/S83/2004(H9N2) 인플루엔자 바이러스의 계통발생학적 관련성을 M 유전자를 대상으로 분석한 것이다. 도 1 내지 3에서, SW는 돼지를 의미하고, CK는 닭을 의미하고, DK는 오리를 의미하고, CD는 붉은갯도요(curlew sandpiper)를 의미하고, TK는 칠면조를 의미한다.
A/Swine/Korea/S81/2004, A/Swine/Korea/S83/2004 및 A/Swine/Korea/S109/2004의 HA 유전자는 아시아계 조류 인플루엔자 바이러스 계통에 속하며, A/Swine/Korea/S10/2004의 HA 유전자는 초기 사람 HA 유전자 계통에 속하여, 사람 인플루엔자 바이러스인 A/WSN/33(H1N1)과 군락을 이룬다. 또는 인플루엔자 바이러스의 종 특이성을 결정하는데 매우 중요한 인자인 M 유전자 분석시, 본 발명의 A/Swine/Korea/S10/2004(H1N1), A/SW/Korea/S109/2004(H9N2) 및 A/SW/Korea/S81/2004(H9N2) 인플루엔자 바이러스는 사람 인플루엔자 바이러스와 군락을 이루며, A/Swine/Korea/S83/2004(H9N2) 인플루엔자 바이러스는 아시아 조류 인플루엔자 바이러스와 군락을 이룬다.
또한, A/Swine/Korea/S109/2004(H9N2)에 면역시킨 토끼의 혈청은 A/Swine/Korea/S81/2004(H9N2) 및 A/Swine/Korea/S83/2004(H9N2) 바이러스에 대하여 혈구응집 억제 활성을 나타내는바, 상기 3종의 바이러스는 교차반응성이 있음을 확인하였다.
본 발명의 돼지 인플루엔자 바이러스들 중, A/Swine/Korea/S10/2004(H1N1)는 본 발명에서 확인한 200 두의 돼지 중 약 37%에서 양성반응을 나타내었고, A/Swine/Korea/S109/2004(H9N2)는 약 36%에서 양성 반응을 나타낸 바, 본 발명에서 분리한 돼지 인플루엔자 바이러스는 국내 돼지에서 유행하는 바이러스 종임을 알 수 있다. 따라서, 본 발명의 돼지 인플루엔자 바이러스에 대한 백신을 제조함으로써, 대부분의 돼지에서 인플루엔자 바이러스에 대한 면역을 형성시킬 수 있으며 또는 돼지를 통한 바이러스 전파 및 신종의 인플루엔자 바이러스의 생성을 억제시킬 수 있다.
또한 본 발명의 돼지 인플루엔자 바이러스들 중, A/Swine/Korea/S10/2004(H1N1), A/Swine/Korea/S109/2004(H9N2) 및 A/Swine/Korea/S81/2004(H9N2)는 항바이러스제인 아만타딘(amantadine)에 대하여 저항성을 갖는다. 상기 아만타딘은 인테그럴 멤브레인 단백질(이온 채널)을 표적으로 하나, 특히 인테그럴 멤브레인 단백질의 26에서 31번째 서열이 항바이러스 활성에 특이적으로 작용한다. 그러나 본 발명의 상기 3종의 바이러스는 31번째 위치에 Ser이 아닌 Asn이 위치하여 아만타딘의 항바이러스 활성에 영향을 받지 않으므로, 이들에 대한 백신이 필수적으로 요구된다.
한편, 본 발명은 돼지 인플루엔자 바이러스에 대한 백신에 관한 것이다. 상기 돼지 인플루엔자 바이러스는, A/Swine/Korea/S10/2004(H1N1), A/Swine/Korea/S109/2004(H9N2), A/Swine/Korea/S81/2004(H9N2) 및 A/Swine/Korea/S83/2004(H9N2)으로 이루어진 군으로부터 선택될 수 있다.
본 발명의 백신은 돼지 인플루엔자 바이러스 또는 이의 항원을 유효성분으로 포함한다. 상기 백신은 사균 백신, 생균 백신, 특이 항원 추출백신(subunit vaccine), 합성 백신(Synthetic vaccine) 또는 유전공학 백신(Genetic enginerring vaccine)일 수 있으나, 생균 백신의 경우 바이러스가 독성이 있는 균주로 환원되어 증식될 수 있으므로 인플루엔자 바이러스에 대한 백신은 사균 백신 특이 항원 추출백신, 합성 백신 또는 유전공학 백신이 바람직하다.
사균 백신은 불활화 백신 또는 사독 백신이라고도 하며, 죽은 바이러스를 포함한 백신이다. 이의 예로는 전 바이러스 백신(whole-virus vaccine)과 분할 백신(split vaccine)이 있으며, 이들은 공지된 방법으로 용이하게 제조가능하다. 예컨대 전 바이러스 백신은 바이러스 전체에 포르말린을 처리하여 제조할 수 있으며, 분할 백신은 바이러스에 에테르를 처리하여 외피만을 분쇄, 수득하여 제조할 수 있다.
특이항원 추출백신은 바이러스의 구성성분 중 면역기능을 일으킬 수 있는 항원 성분만을 추출하여 제조한 백신으로, 바이러스 방어에 필요한 항원 부위에 대해서만 면역형성을 유도함으로써 부작용을 최소화할 수 있다. 예켠대 돼지 인플루엔자 바이러스의 HA 단백질 및/또는 NA 단백질을 추출하여 사용할 수 있다.
합성 백신은 바이러스의 항원 또는 항원결정기만을 화학적으로 합성하거나 또는 재조합 DNA 기술로 생산한 펩타이드를 포함한 백신으로, 예컨대 돼지 인플루엔자 바이러스의 HA 단백질 및/또는 NA 단백질을 합성하여 사용할 수 있다.
유전공학 백신은 바이러스의 병원성을 일으키는 특이 유전자를 변형하거나 제거한 것일 수 있다.
본 발명의 돼지 인플루엔자 바이러스 백신은, 일예로 (a) 돼지 인플루엔자 바이러스를 산란계 유정란에 주입하여 증식시키고, (b) 상기 유정란으로부터 요막 유강을 수득하여 여기에 포르말린, BPL(betapropiolactone) 또는 BEI(BINARYETHILIMINE)를 처리하고 및 (c) 상기 포르말린이 처리된 요막유강으로부터 사독화된 바이러스를 수득하고 이를 가금 바이러스 백신으로 제조하는 단계를 통하여 제조될 수 있다. 각 단계의 상세방법은 하기와 같다.
상기 (a) 단계는, 가금 인플루엔자 바이러스는 5 내지 15일령의 유정란에 주입하고, 유정란은 30 내지 40 ℃에서 24 내지 72시간 두어 바이러스의 증식을 유도한다.
상기 (b) 단계에서는 통상의 방법으로 요막유강을 수득하여 여기에 최종농도 0.005 내지 0.1 (v/w)%로 포르말린, BEI 또는 BPL을 가하고 저온에 방치하여 바이러스를 사독화시킨다.
상기 (c) 단계에서는 포르말린, BEI 또는 BPL이 처리된 요막유강을 원심분리 또는 여과를 통하여 사독화된 바이러스를 수득한다. 수득된 바이러스는 수산화알루미나 겔에 흡착시킨다.
상기한, 백신 제조방법은, 공지의 방법을 채택하거나 또는 일부 수정을 가하여 용이하게 실시가능하다.
또한 본 발명의 백신은 추가적으로 용매, 면역증강제 및 부형제로 이루어진 군으로부터 선택된 1종이상을 더욱 포함할 수 있다. 또한 상기 용매로는 생리식염수 또는 증류수가 있으며, 면역증강제로는 프레운즈 불완전체 또는 완전체 어드쥬번트가 있으며, 부형제로는 알루미늄 포스페이트, 알루미늄 하이드록사이드 또는 알루미늄 포타슘 설페이트(alum)가 있으나, 이에 한정되는 것은 아니며 공지의 물 질이 모두 포함될 수 있다.
본 발명의 백신은 돼지 인플루엔자 바이러스를 375 HA 내지 1000 HA 혈구응집 단위로 포함할 수 있으나, 이에 한정되는 것을 아니다. 다만, 백신의 혈구응집 단위가 375 HA 미만인 경우, 돼지에 효과적으로 항체형성을 유도하지 못할 수 있으며, 1000 HA를 초과하는 경우, 효율대비 비경제적일 수 있다.
본 발명의 백신은 경구형 또는 비경구형 제제로 제조할 수 있으며, 바람직하게는 비경구형 제제인 주사액제로 제조하며, 진피내, 근육내, 복막내, 정맥내, 피하내, 비강 또는 경막외(eidural) 경로로 투여할 수 있다.
본 발명의 백신은 A/Swine/Korea/S10/2004(H1N1), A/Swine/Korea/S109/2004(H9N2), A/Swine/Korea/S81/2004(H9N2) 또는 A/Swine/Korea/S83/2004(H9N2)에 감염될 수 있으며, 상기 인플루엔자 바이러스를 다른 개체에 전파시킬 수 있는 모든 종류의 가금, 조류 및 사람에 사용가능하다. 그 예로는, 돼지, 닭, 오리 또는 터키가 있다.
백신의 투여량은 적용 대상의 종류, 성장정도, 타 약물의 투여여부 및 건강상태에 따라 적절히 조절하여 결정할 수 있으며, 예컨대 체중 1 kg 당 15 HA unit 내지 60 HA unit로 접종할 수 있으며 1차 접종후 2 내지 1달 경과후 2차 접종을 더욱 실시할 수 있다.
이하 본 발명의 실시예를 기재한다. 하기 실시예는 본 발명을 예시하기 위한 것일 뿐 본 발명이 하기 실시예에 한정되는 것은 아니다.
실시예 1: 돼지 인플루엔자 바이러스 검출
1-1. 돼지 인플루엔자 바이러스 검출
국내 돼지 농장에서 사육하는 500 마리의 돼지의 코로부터 시료를 채취하고, 암피실린, 젠타마이신 및 페니실린이 함유된 0.5 mL의 멸균된 포스페이트 완충액(PBS, pH7.4)에 현탁하였다
시료들은 10일령의 닭 수정란의 요막에 접종하여 35 ℃에서 48시간 배양하였고, 요막유액을 회수하였다. 요막유액은 0.5 %의 칠면조 적혈구와 반응시켜 HA(haemagglutination)분석을 통하여 바이러스 존재 여부를 확인하였다.
1-2. 돼지 인플루엔자 바이러스 동정
HA 반응이 확인된 바이러스의 게놈을 분석하기 위하여, RNeasy Mini Kit (Qiagen, Santa Clara, CA)를 이용하여 요막액에 존재하는 바이러스로부터 RNA를 추출하고, 인플루엔자 바이러스에 특이적인 프라이머로 RT-PCR(reverse transcription PCR; SuperScript Preamplification System, Life Technologies, Gaithersburg, MD)을 실시하였다. PCR 산물은 QIAquick PCR 정제 키트(Qiagen)으로 분리하고, pGEM-T 이지 벡터에 클로닝하여 서열분석을 실시하였다. 서열분석은 AmpliTaq DNA 중합효소, FS (Perkin-Elmer, Applied Biosystems, Inc., Foster City, CA) 및 합성 올리고뉴클레오티드를 사용한 BigDye Terminator Cycle Sequencing Ready Reaction Kit로 수행하였고, Lasergene sequence analysis software (DNASTAR, Madison, WI)으로 염기서열과 상기 염기서열로부터 암호되는 단백질 서열을 결정하였다.
하기 표 1은 RT-PCR에 사용된 프라이머 서열이다.
(표 1)
유전자 프라이머 서열 서열번호
PB2 GAT TCA GCT GAT AGT GAG TG 65
AGT AGA AAC AAG GTC GTT TT 66
PB1 CAA CTA GAA AGA AGA TTG AA 67
AGT AGA AAC AAG GCA TTT TT 68
PA AAA GGT AGA CTT TGA CGA TT 69
AGT AGA AAC AAG GTA CTT TT 70
HA TAT TCG TCT CAG GGA GCA AAA GCA GGG G 71
ACA GAG TTC ACC TTG TTT GTA ATC C 72
NP ATG GAG ACT GAT GGA GAA CG 73
ATC AAT TCC ATC ACC ATT GTT CC 74
NA ATG AAT CCA AAC CAG AAA ATA 75
CTT CCT CCA ACT TTT TAT GG 76
M TAT TCG TCT CAG GGA GCA AAA GCA GGT AG 77
ATA TCG TCT CGT ATT AGT AGA AAC AAG GTA GTT TTT 78
NS TAT TCG TCT CAG GGA GCA AAA GCA GGG TG 79
ATA TCG TCT CGT ATT AGT AGA AAC AAG GGT GTT TT 80
염기서열 분석으로, 서로다른 염기서열을 갖는 돼지 인플루엔자 바이로러스 4종을 각각 동정하였다. 각각은 A/Swine/Korea/S10/2004(H1N1), A/Swine/Korea/S109/2004(H9N2), A/Swine/Korea/S81/2004(H9N2) 및 A/Swine/Korea/S83/2004(H9N2)으로 명명하였다.
4종의 바이러스 각각의 유전자 서열은 서열번호 1 내지 32로 기재하였으며, 이로부터 번역되는 단백질 서열은 서열번호 33 내지 64로 기재하였다(표 2).
(표 2)
서열번호
유전자 단백질
A/Swine/Korea/S10/2004(H1N1) PB2 1 33
PB1 2 34
PA 3 35
HA 4 36
NP 5 37
NA 6 38
M 7 39
NS 8 40
A/Swine/Korea/S109/2004(H9N2) PB2 9 41
PB1 10 42
PA 11 43
HA 12 44
NP 13 45
NA 14 46
M 15 47
NS 16 48
A/Swine/Korea/S81/2004(H9N2) PB2 17 49
PB1 18 50
PA 19 51
HA 20 52
NP 21 53
NA 22 54
M 23 55
NS 24 56
A/Swine/Korea/S83/2004(H9N2) PB2 25 57
PB1 26 58
PA 27 59
HA 28 60
NP 29 61
NA 30 62
M 31 63
NS 32 64
1-3. 혈구응집 억제 반응(haemagglutination inhibition)
본 발명에서 동정한 4종의 돼지 인플루엔자 바이러스들중 동일한 H9N2 타입에 속하는 3종의 바이러스간의 교차반응성을 확인하고자 하였다.
A/Swine/Korea/S109/2004(H9N2)로 면역화시킨 토끼로부터 혈청을 수득하고, 이에 A/Swine/Korea/S109/2004(H9N2), A/Swine/Korea/S81/2004(H9N2), A/Swine/Korea/S83/2004(H9N2)과 돼지에서 분리된 조류 인플루엔자 바이러스 A/SW/Korea/S452/2004 및 닭에서 분리된 A/CK/Korea/S1/2003(H9N2) 및 A/CK/Korea/S24/2004(H9N2)을 각각 반응시켜, 혈구응집 억제 역가를 각각 측정하였다. 혈구응집 억제 역가는 40 이상인 경우 양성으로 판단하였다. 그 결과는 하기 표 3과 같다.
(표 3)
혈구응집 억제 역가
A/SW/Korea/S81/2004(H9N2) 640
A/SW/Korea/S83/2004(H9N2) 640
A/SW/Korea/S109/2004(H9N2) 640
A/SW/Korea/S452/2004(H9N2) <10
A/CK/Korea/S1/2003(H9N2) <10
A/CK/Korea/S24/2004(H9N2) <10
표 3에 나타낸 바와 같이, A/Swine/Korea/S109/2004(H9N2)로 면역화된 토끼의 혈청은, A/Swine/Korea/S109/2004(H9N2),A/Swine/Korea/S81/2004(H9N2) 및 A/Swine/Korea/S83/2004(H9N2)에 대하여 혈구응집 억제 역가를 나타내어,교차반응성이 있으나, 그 외 바이러스들은 교차반응성을 나타내지 않는다.
1-4. 혈청학적 역학조사
돼지에서 무작위적으로 혈액을 채취하여 200 개의 혈청 시료를 수득하고, 이를 대상으로 혈구응집 억제 양성여부를 판단하였다. 상기 혈청은 수용체 파괴 효소(receptor destroying enzyme, RDE, Denka Seiken Co., Ltd., Tokyo, Japan)로 처리하여 비특이적 반응을 상쇄시킨 후 혈구응집 억제 분석을 실시하였다. 그 결과, 표 4에 나타낸 바와 같이, A/Swine/Korea/S10/2004(H1N1)에 양성을 보인 개체율은 약 37%, A/Swine/Korea/S109/2004(H9N2)에 양성을 보인 개체는 약 36%를 차지하였고, 조류 인플루엔자 바이러스인 A/Swine/Korea/S452/2004에 양성을 보인 개체는 약 2%로 해당되었다. 즉, 돼지에서 A/Swine/Korea/S10/2004(H1N1) 인플루엔자 바이러스와 A/Swine/Korea/S109/2004(H9N2) 인플루엔자 바이러스의 감염율이 높음을 알 수 있다.
(표 4)
바이러스 혈구응집 억제 양성율, %
A/Swine/Korea/S10/2004(H1N1) 37
A/Swine/Korea/S109/2004(H9N2) 36
A/Swine/Korea/S452/2004(H9N2) 2
실시예 2: 항바이러스제에 대한 저항성 검증
2-1. TCID50 (tisse culture infective dose50)결정
바이러스를 10배수로 희석한 후 MDCK 세포가 자라고 있는 96 프레이트에 접종한 후 바이러스감염을 48시간후 측정하여 TCID50를 측정하였다.
2-2. 아만타딘(amantadine)에 대한 저항성 검증
MDCK 세포는 MEM 배지로 6-웰 플레이트에서 배양하고, 10 ug/mL의 아만타딘 하이드로클로라이드(amantadine hydrochloride, Sigma, St. Louis, Mo) 존재하에 A/Swine/Korea/S10/2004 (human-like H1N1), A/Swine/Korea/S109/2004 (reassorted H9N2) 및 A/Swine/Korea/S452/2004 (avian H9N2)를 각각 102 TCID50/ml으로 1시간 감염시키고, 세포는 0.5 ug/mL의 TPCK-처리된 트립신과 10 ug/mL의 아만타딘 하이드로클로라이드가 포함된 MEM 배지에 배양하였다. 감염 48시간 후 배양물의 상층액을 수득한 후 바이러스 역가를 TCID50으로 결정하였다.
도 4는 아만타딘의 A/Swine/Korea/S10/2004(H1N1), A/Swine/Korea/S109/2004 (H9N2) 및 A/Swine/Korea/S452/2004(H9N2)에 대한 항바이러스 활성을 나타낸 것으 로, □는 아만타딘이 처리되지 않은 상태에서의 바이러스 역가이고, ■는 아만타딘이 처리된 상태에서의 바이러스 역가이다. A/Swine/Korea/S10/2004(H1N1), A/Swine/Korea/S109/2004 (H9N2)는 아만타딘의 처리 여부에 관계없이 일정한 활성을 나타내어 아만타딘에 저항성을 갖는 바이러스임을 알 수 있으나, 조류 인플루엔자 바이러스 유전자를 가진 A/Swine/Korea/S452/2004(H9N2)는 아만타딘에 감수성을 가져 아만타딘 처리시 바이러스 역가가 현저히 감소하였다.
이상 살펴본 바와 같이, 본 발명은 신종의 돼지 인플루엔자 바이러스를 제공하여, 돼지를 인플루엔자 바이러스에 대한 면역을 효과적으로 형성시킬 수 있으며 따라서 돼지 농장에서의 인플루엔자 바이러스에 의한 질환 발병을 현저히 감소시켜 경제적인 손실을 방지할 수 있다.
<110> VACCINE LEADER Choong Ang Vaccine Lab. <120> A/SW/KOREA/S109/2004(H9N2) INFLUENZA VIRUS AND VACCINE THEREFOR <130> dpp20045989kr <160> 80 <170> KopatentIn 1.71 <210> 1 <211> 2265 <212> DNA <213> PB2 gene of A/SW/KOREA/S10/04(H1N1) INFLUENZA VIRUS <400> 1 ataaaggaac taagagattt gatgtcacag tcccgcactc gcgagatact gacgaaaacc 60 actgtggacc atatggccat aatcaagaaa tacacatcgg gaaggcagga gaagaacccc 120 gctctcagaa tgaaatggat gatggcaatg aaatacccga ttacagctga caaaagaata 180 atggagatga tccctgaaag aaatgagcaa ggtcaaactc tttggagcaa aacgaatgat 240 gctggatcag ataaggtaat ggtatcacct ctagctgtga cgtggtggaa tagaaatgga 300 ccaacaacaa gcacaatcca ttatcccaag gtatataaaa cttactttga gaaggttgaa 360 aggttgaaac acggaacctt tggccccatt catttccgga atcaagtcaa aatacgccgc 420 acggttgaca caaacccggg ccatgcagac ctcagtgcta aagaagcaca agatgttatc 480 atggaggtcg ttttcccaaa tgaagttgga gccaggatac tgacatcaga atcacaattg 540 acaataacaa aggaaaagaa agaggaactc caggattgta agattgctcc tttgatggtg 600 gcgtacatgt tagaaagaga actggttcgc aagaccagat tcctaccagt ggcgggcggg 660 acaagcagcg tgtatataga agtattacat ttgactcaag gaacgtgctg ggagcagatg 720 tacacaccag gaggggaggt gaggaatgat gatgttgacc agagtttaat cattgctgct 780 agaaacattg taaggagagc aacagtatca gcagacccat tggcctcgct cttggagatg 840 tgtcatggta cgcaaatagg tggaataaga atggtagaca tccttagaca gaacccaaca 900 gaagagcaag ccgtggatat atgcaaggca gcaatgggcc taagaatcag ttcatctttc 960 agctttggag gtttcacttt caaaagaaca agtgggtctt ctgtcaaaaa ggaagaagaa 1020 gtgcttacag gcaacctcca gacattgaaa ataagagtgc atgagggata tgaggaattc 1080 acaatggttg ggcgaagagc aacagccctt ctaaggaaag caaccagaag gctgattcaa 1140 ctgatagtga gtgggagaga cgaacaatca atcgctgaag cgatcattgt agcaatggta 1200 ttttcacaag aggactgcat gataaaggca gtccgaggtg atttgaactt catgaacaga 1260 gcgaaccaac gactgaaccc catgcaccag cttctgaggc acttccaaaa agatgcaaaa 1320 gtgttgtttc agaactgggg aattgaaccc atcgacaatg tcatggggat gattggaata 1380 ttgcctgaca tgactcccag cacagagatg tcactaagag gagtgagagt cagcaagatg 1440 ggagtggacg agtactccag cactgaaaga gtggttgtga gtattgatcg tttcttgagg 1500 gttcgggatc agagggggaa catacttcta tcccctgaag aagttagtga gacacaaggg 1560 acggaaaagt taacaataac atattcatcg tctatgatgt gggagattaa cggcccggaa 1620 tcagtgctag ttaacacata ccaatggatc attaggaatt gggagactgt gaagattcag 1680 tggtctcaag atcctactat gttatacaat aaggtggaat ttgaaccctt ccaatctctg 1740 gtacctaaag ctgccagagg ccaatatagt ggatttgtga gaacgctatt ccaacaaata 1800 cgtgatgtac tggggacatt tgacactgtt caaataataa agctgctacc atttgccgca 1860 gccccaccag agcagagtag gatgcagttt tcttctctga ctgtgaatgt aagaggctca 1920 ggaatgagaa tccttgtgag aggcaactcc cctgtgttta actacaacaa ggcaaccaag 1980 aggcttacag tcctcgggaa ggatgcaggc gcactcacag aagacccaga tgagggaaca 2040 gcaggagtgg aatccgcagt attaagagga ttcctaattc taggcaaaga agacaagaga 2100 tacggaccag cattgagcat caacgaattg agcaatcttg cgaaaggaga gaaagccaat 2160 gtattgatag ggcaaggaga cgtagtgttg gtaatgaaac ggaaacggga ctctagcata 2220 cttactgaca gccagacagc gaccaaaaga attcgaatgg ccatc 2265 <210> 2 <211> 2341 <212> DNA <213> PB1 gene of A/SW/KOREA/S10/04(H1N1) INFLUENZA VIRUS <400> 2 agcgaaagca ggcaaaccat ttgaatggat gtcaatccga ctttactttt cttaaaagtg 60 ccagcacaaa atgctataag cacaactttc ccttatactg gagaccctcc ttacagccat 120 gggacaggaa caggatacac catggatact gtcaacagga cacatcagta ctcagaaagg 180 ggaagatgga caacaaacac cgaaactgga gcaccgcaac tcaacccgat tgatgggcca 240 ctgccagaag acaatgaacc aagtggttat gcccaaacag attgtgtatt ggaagcaatg 300 gccttccttg aggaatccca tcctggtatc tttgagacct cgtgtcttga aacgatggag 360 gttgttcagc aaacacgagt ggacaagctg acacaaggcc gacagaccta tgactggact 420 ctaaatagaa accagcctgc tgcaacagca ttggccaaca caatagaagt gttcagatca 480 aatggcctca cggccaatga acctggaagg ctcatagact tccttaagga tgtagtggag 540 tcaatgaaca aagaagaaat ggagatcaca actcattttc agagaaagag acgagtgaga 600 gacaatatga ctaagaaaat ggtgacacag agaacaatag gtaaaaggaa gcagagattg 660 aacaaaagga gttatctaat tagggcatta accctgaaca caatgaccaa agatgctgag 720 agagggaagc taaaacggag agcaattgca accccaggga tgcaaataag ggggtttgta 780 tactttgttg agacactagc aaggagtata tgtgagaaac ttgaacaatc aggattgcca 840 gttggaggca atgagaagaa agcaaagttg gcaaatgttg taaggaagat gatgaccaat 900 tctcaggaca ctgaaatttc tttcaccatc actggagata acaccaaatg gaacgaaaat 960 cagaaccctc ggatgttttt ggccatgatc acatatataa ccagaaatca gcccgaatgg 1020 ttcagaaatg ttctaagtat tgctccaata atgttctcaa acaaaatggc gagactggga 1080 aaggggtaca tgtttgagag caagagtatg aaaattagaa ctcaaatacc tgcagaaatg 1140 ctagcaagca tcgatttgaa atacttcaat gattcaacta gaaagaagat tgaaaaaatc 1200 cggccgctct taatagatgg gactgcatca ttgagccctg gaatgatgat gggcatgttc 1260 aatatgttaa gtactgtatt aggcgtctcc atcctgaatc ttggacaaaa gagacacacc 1320 aagactactt actggtggga tggtcttcaa tcttctgatg attttgctct gattgtgaat 1380 gcacccaatc atgaagggat tcaagccgga gtcaacaggt tttatcgaac ctgtaagcta 1440 cttggaatta atatgagcaa gaaaaagtct tacataaaca gaacaggtac atttgaattc 1500 acaagttttt tctatcgtta tgggtttgtt gccaatttcg gcatggagct tcccagcttt 1560 ggggtgtctg ggatcaacga gtctgcggac atgagtattg gagttactgc catcaaaaac 1620 aatatgataa acaatgatct tggtccagca accgctcaaa tggcccttca actgttcatc 1680 aaagattaca ggtacacgta ccggtgccat agaggtgaca cacaaataca aacccgaaga 1740 tcatttgaaa taaagaaact gtgggagcaa acccattcca aagctggact gctggtctcc 1800 gacggaggcc caaatttata caacattaga aatctccaca ttcctgaagt ctgcttgaaa 1860 tgggaattaa tggatgagga ttaccagggg cgtttatgca acccactgaa cccatttgtc 1920 aaccataaag acattgaatc agtgaacaat gcagtgataa tgccagcaca tggtccagcc 1980 aaaaacatgg ggtatgatgc tgttgcaacc acacactcct gggatcccca aagaaatcga 2040 tccatcttga atacaagcca aagaggaata cttgaagatg aacaaatgta ccaaaagtgc 2100 tgcaacttat ttgaaaaatt cttccccagc agttcataca gaagaccagt cgggatatcc 2160 agtatggtgg aggctatggt ttccagagcc cgaattgatg cacgaattga ttccgaatct 2220 ggaaggataa agaaagagga gttcactgag atcatgaaga tctgttccac cattgaagag 2280 ctcagacggc aaaaatagtg aatttagctt gtccttcatg aaaaaatgcc ttgtttctac 2340 t 2341 <210> 3 <211> 2233 <212> DNA <213> PA gene of A/SW/KOREA/S10/04(H1N1) INFLUENZA VIRUS <400> 3 agcgaaagca ggtactgatt caaaatggaa gattttgtgc gacaatgctt caatccgatg 60 attgtcgagc ttgcggaaaa ggcaatgaaa gagtatggag aggacctgaa agtcgaaaca 120 aacaaatttg cagcaatatg cactcacttg gaagtgtgct tcatgtattc agattttcac 180 ttcatcgatg agcaaggcga gtcaatagtc gtagaacttg gcgatccaaa tgcacttttg 240 aagcacagat ttgaaataat cgagggaaga gatcgcacaa tagcctggac agtaataaac 300 agtatttgca acactacagg ggctgagaaa ccaaagtttc taccagattt gtatgattac 360 aagaagaata gattcatcga aattggagta acaaggagag aagttcacat atactatctg 420 gaaaaggcca ataaaattaa atctgagaag acacacatcc acattttctc attcactggg 480 gaggaaatgg ccacaaaggc cgactacact ctcgatgaag aaagcagggc taggatcaaa 540 accaggctat tcaccataag acaagaaatg gctagcagag gcctctggga ttcctttcgt 600 cagtccgaga gaggcgaaga gacaattgaa gaaagatttg aaatcacagg aacaatgcgc 660 aagcttgccg accaaagtct cccgccaaac ttctccagcc ttgaaaaatc tagagcctat 720 gtggatggat tcgaaccgaa cggctacatt gagggcaagc tttctcaaat gtccaaagaa 780 gtaaatgcta gaattgaacc ttttttgaaa tcaacaccac gaccacttag acttccggat 840 gggcctccct gttctcagcg gtccaaattc ctgctgatgg atgccttaaa attaagcatt 900 gaggacccaa gtcatgaggg agaggggata ccgctatatg atgcaatcaa atgcatgaga 960 acattctttg gatggaagga acccaatgtt gttaaaccac acgaaaaggg aataaatcca 1020 aattatcttc tgtcatggaa gcaagtactg gcagaactgc aggacattga gaatgaggag 1080 aaaattccaa ggactaaaaa tatgaagaaa acgagtcagt taaagtgggc acttggtgag 1140 aacatggcac cagaaaaggt agactttgac gattgtaaag atgtaggcga tttgaagcaa 1200 tatgatagtg atgaaccaga attgaggtcg cttgcaagtt ggattcagaa tgagttcaac 1260 aaggcatgtg aactgaccga ttcaagctgg atagagctcg atgagattgg agaagatgcg 1320 gctccaattg gacacattgc aagcatgaga aggaattatt tcacagcaga ggtgtctcat 1380 tgcagagcca cagaatacat aatgaagggg gtgtacatca atactgcctt gcttaatgca 1440 tcctgtgcag caatggatga tttccaatta attccaatga taagcaagtg tagaactaag 1500 gagggaaggc gaaagaccaa tttgtacggt ttcatcataa aaggaagatc ccacttaagg 1560 aatgacaccg atgtggtaaa ctttgtgagc atggagtttt ccctcactga cccaagactt 1620 gaaccacaca aatgggagaa gtactgtgtt cttgaggtag gagatatgct tctaagaagt 1680 gccataggcc atgtgtcaag gcctatgttc ttgtatgtga ggacaaatgg aacctcaaaa 1740 attaaaatga aatgggggat ggaaatgagg cgttgcctcc ttcagtcact tcaacaaatc 1800 gagagtatga ttgaagctga gtcctctgtc aaggagaaag acatgaccaa agagttcttt 1860 gaaaacaaat cagaaacatg gcccgttgga gagtccccca aaggagtgga ggaaggttcc 1920 attgggaagg tctgcagaac tttattggca aagtcggtat tcaacagctt gtatgcatct 1980 ccccaactag aaggattttc agctgaatca agaaaactgc ttcttatcgt tcaggctctt 2040 agggacaacc tggaacctgg gacctttgat cttggggggc tatatgaagc aattgaggag 2100 tgcctgatta atgatccctg ggttttgctt aatgcttctt ggttcaactc cttcctcaca 2160 catgcattga gatagttgtg gcaatgctac tatttgctat ccatactgtc caaaaaagta 2220 ccttgtttct act 2233 <210> 4 <211> 869 <212> DNA <213> HA gene of A/SW/KOREA/S10/04(H1N1) INFLUENZA VIRUS <400> 4 atgaaggcaa aactactggt cctgttatat gcatttgtag ctacagatgc agacacaata 60 tgtataggct accatgcgaa caactcaacc gacactgttg acacaatact cgagaagaat 120 gtggcagtga cacattctgt taacctgctc gaagacagcc acaacgggaa actatgtaaa 180 ttaaaaggaa tagccccact acaattgggg aaatgtaaca tcaccggatg gctcttggga 240 aatccagaat gcgactcact gcttccagcg agatcatggt cctacattgt agaaacacca 300 aactctgaga atggagcatg ttatccagga gatctcatcg actatgagga actgagggag 360 caattgagct cagtatcatc attaggaaga ttcgaaatat ttcccaagga aagttcatgg 420 cccaaccaca cattcaacgg agtaacagta tcatgctccc ataggggaaa aagcagtttt 480 tacagaaatt tgctatggct gacgaagaag ggggattcat acccaaagct gaccaattcc 540 tatgtgaaca ataaagggaa agaagtcctt gtactatggg gtgttcatca cccgtctagc 600 agtgatgagc aacagagtct ctatagtaat ggaaatgctt atgtctctgt agcgtcttca 660 aattataaca ggagattcac cccggaaata gctgcaaggc ccaaagtaag agatcaacat 720 gggaggatga actattactg gaccttgcta gaacccggag acacaataat atttgaggca 780 actggtaatc taatagcacc atggtatgct ttcgcactga gtagagggtt tgagtccggc 840 atcatcacct caaacgcgtc aatgcatga 869 <210> 5 <211> 543 <212> DNA <213> NP gene of A/SW/KOREA/S10/04(H1N1) INFLUENZA VIRUS <400> 5 ggagactgat ggagaacgcc agaatgccac tgaaatcaga gcatctgtcg gaaaaatgat 60 tgatggaatt ggacgattct acatccaaat gtgcaccgaa cttaaactca gtgattatga 120 gggacggctg attcagaaca gcttaacaat agagagaatg gtgctctctg cttttgacga 180 gaggaggaat aaatatctag aagaacatcc cagtgcgggg aaagatccta agaaaactgg 240 aggacctata tacaggagag tagatggaaa gtggaggaga gaactcatcc tttatgacaa 300 agaagaaata agacgaatct ggcgccaagc taataatggt gacgatgcaa cggctggtct 360 gactcacatg atgatctggc actccaattt gaatgatgca acttaccaga ggacaagaac 420 tcttgttcgc acaggaatgg atcccaggat gtgctcactg atgcagggtt caaccctccc 480 taggaggtct ggggccgcag gtgctgcagt caaaggagtt ggaacaatgg tgagggaatt 540 gat 543 <210> 6 <211> 1097 <212> DNA <213> NA gene of A/SW/KOREA/S10/04(H1N1) INFLUENZA VIRUS <400> 6 atgaatccaa accagaaaat aataaccatt gggtcaatct gtatggtagt cggaataatt 60 agcctaatat tgcaaatagg aaatataatc tcaatatgga ttagccattc aattcaaacc 120 ggaaatcaaa accatactgg aatatgcaac caaggcagca tcacctataa agttgttgct 180 gggcaggact caacttcagt gatattaacc ggcaattcat ctctttgtcc catccgtggg 240 tgggctatac acagcaaaga caatggcata agaattggtt ccaaaggaga cgtttttgtc 300 ataagagagc cttttatttc atgttctcac ttggaatgca ggaccttttt tctgactcaa 360 ggcgccttac tgaatgacaa gcattcaagg gggaccttta aggacagaag cccttatagg 420 gccttaatga gctgccctgt cggtgaagct ccgtccccgt acaattcaag gtttgaatcg 480 gttgcttggt cagcaagtgc atgtcatgat ggaatgggct ggctaacaat cggaatttct 540 ggtccagatg atggagcagt ggctgtatta aaatacaacc gcataataac tgaaaccata 600 aaaagttgga ggaagaatat attgagaaca caagagtctg aatgtacctg tgtaaatggt 660 tcatgtttta ccataatgac cgatggccca agtgatgggc tggcctcgta caaaattttc 720 aagatcgaga aggggaaggt tactaaatcg atagagttga atgcacctaa ttctcactac 780 gaggaatgtt cctgttaccc tgataccggc aaagtgatgt gtgtgtgcag agacaattgg 840 cacggttcga accgaccatg ggtgtccttc gaccaaaacc tagattataa aataggatac 900 atctgcagtg gggttttcgg tgacaacccg cgtcccaaag atggaacagg cagctgtggc 960 ccagtgtctg ctgatggagt aaacggagta aagggatttt catataagta tggcaatggt 1020 gtttggatag gaaggactaa aagtgacagt tccagacatg ggtttgagat gatttgggat 1080 cctaatggat ggacaga 1097 <210> 7 <211> 1027 <212> DNA <213> M gene of A/SW/KOREA/S10/04(H1N1) INFLUENZA VIRUS <400> 7 agcaaaagca ggtagatatt gaaagatgag tcttctaacc gaggtcgaaa cgtacgttct 60 ctctatcgtc ccgtcaggcc ccctcaaagc cgagatcgca cagagacttg aagatgtctt 120 tgcagggaag aacaccgatc ttgaggttct catggaatgg ctaaagacaa gaccaatcct 180 gtcacctctg actaagggga ttttaggatt tgtgttcacg ctcaccgtgc ccagtgagcg 240 gggactgcag cgtagacgct ttgtccaaaa tgctcttaat gggaacggag atccaaataa 300 catggacaaa gcagttaaac tgtataggaa gcttaagagg gagataacat tccatggggc 360 caaagaaata gcactcagtt attctgctgg tgcacttgcc tgttgtatgg gcctcatata 420 caacaggatg ggggctgtga ccactgaagt ggcatttggc ctggtatgcg caacctgtga 480 acagattgct gactcccagc atcggtctca taggcaaatg gtgacaacaa ccaatccact 540 aatcagacat gagaacagaa tggttctagc cagcactaca gctaaggcta tggagcaaat 600 ggctggatcg agtgagcaag cagcagaggc catggatatt gctagtcagg ccaggcaaat 660 ggtgcaggcg atgagaaccg ttgggactca tcctagctcc agtgctggtc taaaagatga 720 tcttcttgaa aatttacagg cctatcagaa acgaatgggg gtgcagatgc aacgattcaa 780 gtgatcctct cgtcattgca gcaaatatca ttggaatctt gcacttgata ttgtggattc 840 ttgatcgtct ttttttcaaa tgcatttatc gtcgctttaa atacggtttg aaaagagggc 900 cttctaccga aggagtgcca gagtctatga gggaagaata tcgaaaggaa cagcagaatg 960 ctgtggatgt tgacgatggt cattttgtca acatagagct ggagtaaaaa actaccttgt 1020 ttctact 1027 <210> 8 <211> 862 <212> DNA <213> NS gene of A/SW/KOREA/S10/04(H1N1) INFLUENZA VIRUS <400> 8 agcaaaagca gggtgacaaa gacataatgg atccaaacac tgtgtcaagc tttcaggtag 60 attgctttct ttggcatgtc cgcaaaagag ttgcagacca agaactaggt gatgccccat 120 tccttgatcg gcttcgccga gatcagaagt ccctaagagg aagaggcagc actcttggtc 180 tggacatcga aacagccacc cgtgctggaa agcaaatagt ggagcggatt ctgaaggaag 240 aatctgatga ggcactcgaa atgaccatgg cctctgtacc tgcatcgcgc tacctaactg 300 acatgactct tgaggaaatg tcaaggcact ggttcatgct catgcccaag cagaaagtgg 360 caggccctct ttgtatcaga atggaccagg cgatcatgga taagaacatc atactgaaag 420 cgaacttcag tgtgattttt gaccggctgg agactctaat attactaagg gccttcaccg 480 aagaggggac aattgttggc gaaatttcac cactgccctc tcttccagga catactgatg 540 aggatgtcaa aaatgcagtt ggggtcctca tcggaggact tgaatggaat aataacacag 600 ttcgagtctc tgaaactcta cagagattcg cttggagaag cagtaatgag aatgggagac 660 ctccactcac tccaaaacag aaacggaaaa tggcgggaac aattacgtca gaagtttgaa 720 gaaataatgt ggttgattga agaagtgaga cacagactga agataacaga gatagttttg 780 agcaaataac atttatgcag ctacactatt gctgagtgag cagagataag actactcgtt 840 tcagcttatt taataataaa aa 862 <210> 9 <211> 1124 <212> DNA <213> PB2 gene of A/SW/KOREA/S109/04(H1N1) INFLUENZA VIRUS <400> 9 cgaacagtcg attgccgaag caataattgt agcaatggta ttttcacaag aggactgcat 60 gataaaggca gtccgaggtg atttgaactt catgaacaga gcgaaccaac gactgaaccc 120 catgcaccag cttctgaggc acttccaaaa agatgcaaaa gtgttgtttc agaactgggg 180 aattgaaccc atcgacaatg tcatggggat gattggaata ttgcctgaca tgactcccag 240 cacagagatg tcactaagag gagtgagagt cagcaagatg ggagtggacg agtactccag 300 cactgaaaga gtggttgtga gtattgatcg tttcttgagg gttcgggatc agagggggaa 360 catacttcta tcccctgaag aagttagtga gacacaaggg acggaaaagt taacaataac 420 atattcatcg tctatgatgt gggagattaa cggcccggaa tcagtgctag ttaacacata 480 ccaatggatc attaggaatt gggagactgt gaagattcag tggtctcagg atcctactat 540 gttatacaat aaggtggaat ttgaaccctt tcaatctctg gtacctaaag ctgccagagg 600 ccaatatagt ggatttgtga gaacgctatt ccaacaaatg cgtgatgtac cggggacatt 660 tgacactgtt caaataataa agctgctacc atttgccgca gccccaccag agcagagtag 720 gatgcagttt tcttctctga ctgtgaatgt aagaggctca ggaatgagaa tccttgtgag 780 aggcaactcc cctgtgttta actacaacaa ggcaaccaag aggcttacag tcctcgggaa 840 ggatgcaggc gcactcacag aagacccaga tgagggaaca gcaggagtgg aatccgcagt 900 attaagagga ttcctaattc taggcaaaga agacaagaga tacggaccag cattgagcat 960 caacgaattg agcaatcttg cgaaaggaga gaaagccaat gtattgatag ggcaaggaga 1020 cgtagtgttg gtaatgaaac ggaaacggga ctctagcata cttactgaca gccagacagc 1080 gaccaaaaga attcgaatgg ccatcaatta gtgtcgaatt gttt 1124 <210> 10 <211> 2341 <212> DNA <213> PB1 gene of A/SW/KOREA/S109/04(H1N1) INFLUENZA VIRUS <400> 10 agcgaaagca ggcaaaccat ttgaatggat gtcaatccga ctttactttt cttaaaagtg 60 ccagcacaaa atgctataag cacaactttc ccttatactg gagaccctcc ttacagccat 120 gggacaggaa caggatacac catggatact gtcaacagga cacatcagta ctcagaaagg 180 ggaagatgga caacaaacac cgaaactgga gcaccgcaac tcaacccgat tgatgggcca 240 ctgccagaag acaatgaacc aagtggttat gcccaaacag attgtgtatt ggaagcaatg 300 gccttccttg aggaatccca tcctggtatc tttgagacct cgtgtcttga aacgatggag 360 gttgttcagc aaacacgagt ggacaagctg acacaaggcc gacagaccta tgactggact 420 ctaaatagga accagcctgc tgcaacagca ttggccaaca caatagaggt gttcagatca 480 aatggcctca cggccaatga atctggaagg ctcatagact tccttaagga tgtaatggag 540 tcaatgaaca aagaagaaat ggagatcaca actcattttc agagaaagag acgagtgaga 600 gacaatatga ctaagaaaat ggtgacacag agaacaatag gtaaaaggaa gcagagattg 660 aacaaaagga gttatctaat tagggcatta accctgaaca caatgaccaa agatgctgag 720 agagggaagc taaaacggag agcaattgca accccaggga tgcaaataag ggggtttgta 780 tactttgttg agacactagc aaggagtata tgtgagaaac ttgaacaatc aggattgcca 840 gttggaggca atgagaagaa agcaaagttg gcaaatgttg taaggaagat gatgaccaat 900 tctcaggaca ctgaaatttc tttcaccatc actggagata acaccaaatg gaacgaaaat 960 cagaaccctc ggatgttttt ggccatgatc acatatataa ccagaaatca gcccgaatgg 1020 ttcagaaatg ttctaagtat tgctccaata atgttctcaa acaaaatggc gagactggga 1080 aaggggtaca tgtttgagag caagagtatg aaaattagaa ctcaaatacc tgcagaaatg 1140 ctagcaagca tcgatttgaa atacttcaat gattcaacta gaaagaagat tgaaaaaatc 1200 cggccgctct taatagatgg gactgcatca ttgagccctg gaatgatgat gggcatgttc 1260 aatatgttaa gtactgtatt aggcgtctcc atcctgaatc ttggacaaaa gagacacacc 1320 aagactactt actggtggga tggtcttcaa tcttctgatg attttgctct gattgtgaat 1380 gcacccaatc atgaagggat tcaagccgga gtcaacaggt tttatcgaac ctgtaagcta 1440 ctcggaatta atatgagcaa gaaaaagtct tacataaaca gaacaggtac atttgaattc 1500 acaagttttt tctatcgtta tgggtttgtt gccaaattca gcgtggagct tcccagcttt 1560 ggggtgtctg ggatcaacga gtctgcggac atgagtattg gagttactgt catcaaaaac 1620 aatatgataa acaatgatct tggtccagca accgctcaaa tggcccttca gctgttcatc 1680 aaagattaca ggtacacgta ccggtgccat agaggtgaca cacaaataca aacccgaaga 1740 tcatttgaaa taaagaaacc gtgggagcaa acccattcca aagctggact gctggtctcc 1800 gacggaggcc caaatttata caacattaga aatctccaca ttcctgaagt ctgcttgaaa 1860 tgggaattaa tggatgagga ttaccagggg cgtttatgca acccactgaa cccatttgtc 1920 aaccataaag acattgaatc agtgaacaat gcagtgataa tgccagcaca tggtccagcc 1980 aaaaacatgg agtatgatgc tgttgcaaca acacactcct ggatccccaa aagaaatcga 2040 tccatcttga atacaagcca aagaggaata cttgaagatg aacaaatgta ccaaaagtgc 2100 tgcaacttat ttgaaaaatt cttccccagc agttcataca gaagaccagt cgggatatcc 2160 agtatggtgg aggctatggt ttccagagcc cgaattgatg cacgaattga tttcgaatct 2220 ggaaggataa agaaagagga gttcactgag atcatgaaga tctgttccac cattgaagag 2280 ctcagacggc aaaaatagtg aatttagctt gtccttcatg aaaaaatgcc ttgtttctac 2340 t 2341 <210> 11 <211> 2248 <212> DNA <213> PA gene of A/SW/KOREA/S109/04(H1N1) INFLUENZA VIRUS <400> 11 atggaagatt ttgtgcgaca atgcttcaat ccgatgattg tcgagcttgc ggaaaaggca 60 atgaaagagt atggagagga cctgaaaatc gaaacaaaca aatttgcagc aatatgcact 120 cacttggaag tgtgcttcat gtattcagat tttcacttca tcgatgagca aggcgagtca 180 atagtcgtag aacttggcga tccaaatgca cttttgaagc acagatttga aataatcgag 240 ggaagagatc gcacaatagc ctggacagta ataaacagta tttgcaacac tacaggggct 300 gagaaaccaa agtttctacc agatttgtat gattacaaga agaatagatt catcgaaatt 360 ggagtaacaa ggagagaagt tcacatatac tatctggaaa aggccaataa aattaaatct 420 gagaagacac acatccacat tttctcattc actggggagg aaatggccac aaaggccgac 480 tacactctcg atgaagaaag cagggctagg atcaaaacca ggctattcac cataagacaa 540 gaaatggcta gcagaggcct ctgggattcc tttcgtcagt ccgagagagg cgaagagaca 600 attgaagaaa gatttgaaat cacaggaaca atgcgcaagc ttgccgacca aagtctcccg 660 ccaaacttct ccagccttga aaaatttaga gcctatgtgg atggattcga accgaacggc 720 tacattgagg gcaagctttc tcaaatgtcc aaagaagtaa atgctagaat tgaacctttt 780 ttgaaatcaa caccacgacc acttagactt ccggatgggc ctccctgttc tcagcggtcc 840 aaatccctgc tgatggatgc cttaaaatta agcattgagg acccaagtca tgagggagag 900 gggataccgc tatatgatgc aatcaaatgc atgagaacat tctttggatg gaaggaaccc 960 aatgttgtta aaccacacga aaagggaata aatccaaatt atcttctgtc atggaagcaa 1020 gtactggcag aactgcagga cattgagaat gaggagaaaa ttccaaggac taaaaatatg 1080 aagaaaacga gtcagttaaa gtgggcactt ggtgagaaca tggcaccaga aaaggtagac 1140 tttgacgatt gtaaagatgt aggcgatttg aagcaatatg atagtgatga accagaattg 1200 aggtcgcttg caagttggat tcagaatgag ttcaacaagg catgtgaact gaccgattca 1260 agctggatag agctcgatga gattggagaa gatgcggctc caattgaaca cattgcaagc 1320 atgagaagga attatttcac agcagaggtg tctcattgca gagccacaga atacataatg 1380 aagggggtgt acatcaatac tgccttgctt aatgcatcct gtgcagcaat ggatgatttc 1440 caactaattc caatgataag caagtgtaga actaaggagg gaaggcgaaa gaccaatttg 1500 tacggtttca tcataaaagg aagatcccac ttaaggaatg acaccgatgt ggtaaacttt 1560 gtgagcatgg agttttccct cactgaccca agacttgaac cacacaaatg ggagaagtac 1620 tgtgttcttg aggtaggaga tatgcttcta agaagtgcca taggccatgt gtcaaggcct 1680 atgttcttgt atgtgaggac aaatggaacc tcaaaaatta aaatgaaatg ggggatggaa 1740 atgaggcgtt gcctccttca gtcacttcaa caaatcgaga gtatgattga agctgagtcc 1800 tctgtcaagg agaaagacat gaccaaagag ttctttgaaa acaaatcaga aacatggccc 1860 gttggagagt cccccaaagg agtggaggaa ggttccattg ggaaggtctg cagaacttta 1920 ttggcaaagt cggtattcaa cagcttgtat gcatctccac aactagaagg attttcagct 1980 gaatcaagaa aactgcttct tatcgttcag gctcttaggg acaacctgga acctgggacc 2040 tttgatcttg gggggctata tgaagcaatt gaggagtgcc tgattaatga tccctgggtt 2100 ttgcttaatg cttcttggtt caactccttc ctcacacatg cattgagata gttgtggcaa 2160 tgctactatt tgctatccat actgtccaaa aaagtacctt gtttctacta atcactagtg 2220 aattcgcggc cgcctgcagg tcgaccat 2248 <210> 12 <211> 1104 <212> DNA <213> HA gene of A/SW/KOREA/S109/04(H1N1) INFLUENZA VIRUS <400> 12 atggaagtaa tagcactaat agctatactg gtagtgacag gaacgagcaa tgctgataaa 60 atttgcattg gataccagtc aacaaactcc acagaaactg ttgatacact agtagaaacc 120 aatgtccctg tgacacatgc caaagaactg cttcacacag agcacaatgg aatgttatgt 180 gcaacaaatc aggggcgtcc tcttatccta gacacctgca ccgttgaagg gttggtgtat 240 ggcaatcctt cctgtgattt gctactggga ggaaaagaat ggtcttacat tgtcaaaaga 300 tcatcagcca ttaatgggat gtgttaccct ggaagggtag agaatctgga agaactaaga 360 tcttttttca gttctgctcg ctcctacaaa agactcctgc tctttccaga ccgcacttgg 420 aatgtgactt acactggaac aagcaaagca tgctcaggtt cattctacag aagtatgaga 480 tggctgacac acaagagcaa ttcttaccct attcaagacg ctcaatatac caacgattgg 540 ggaaagaata ttctcttcat gtggggcata caccatccac ctactgatac tgagcaaatg 600 aatctataca aaaaagctga tacaacaacg agcataacaa cggaagatat caatcggact 660 ttcaaaccag tgataggacc aaggcccctt gtcaatggtc aacaagggag gattgattat 720 tattggtcag tactaaagcc aggccagaca ttgcgaataa gatccaatgg aaatctaatt 780 gctccatggt atggacacat tccttcagga gaaagccatg gaagaatcct aaagaccgat 840 ttaaatagtg gcaactgcgt agtacagtgc caaactgaga aaggtggttt gaatacgaca 900 ttgccattcc acaatgtcag caagtatgca tttgggaact gccccaaata tgttggagtg 960 aagagtctca agctggcagt tggtctaaga aatgtgcctg ctgcatcagg tagagggcta 1020 tttggtgcca tagctggatt catagaagga ggttggccag ggctagttgc aggctggtac 1080 gggtttcagc attcaaatga tcaa 1104 <210> 13 <211> 543 <212> DNA <213> NP gene of A/SW/KOREA/S109/04(H1N1) INFLUENZA VIRUS <400> 13 ggagactgat ggagaacgcc agaatgccac tgaaatcaga gcatctgtcg gaaaaatgat 60 tgatggaatt ggacgattct acatccaaat gtgcaccgaa cttaaactca gtgattatga 120 gggacggctg attcagaaca gcttaacaat agagagaatg gtgctctctg cttttgacga 180 gaggaggaat aaatatctag aagaacatcc cagtgcgggg aaagatccta agaaaactgg 240 aggacctata tacaggagag tagatggaaa gtggaggaga gaactcatcc tttatgacaa 300 agaagaaata agacgaatct ggcgccaagc taataatggt gacgatgcaa cggctggtct 360 gactcacatg atgatctggc actccaattt gaatgatgca acttaccaga ggacaagagc 420 tcttgttcgc acaggaatgg atcccaggat gtgctcactg atgcagggtt caaccctccc 480 taggaggtct ggggccgcag gtgctgcagt caaaggagtt ggaacaatgg tgatggaatt 540 gat 543 <210> 14 <211> 1407 <212> DNA <213> NA gene of A/SW/KOREA/S109/04(H1N1) INFLUENZA VIRUS <400> 14 atgaatccaa atcagaaaat aataacaatt ggctctgtct ctctaaccat tgccacagta 60 tgtttcctca tgcagattgc cattctagca acgactgtaa cactgcactt caagcaaaat 120 gaaggcagca ttccctcgaa caatcaagta gttccatgtg aaccaatcat aatagaaagg 180 aacataacag aaatagtgta tttaaataat actatcatag agaaagaact ttgccctaaa 240 gtggtaaaat ataggagttg gtcgaaaccg cagtgtcaga tcacagggtt tgctcctttc 300 tccaaggacg actcaattcg gctgtctgct ggtgggagca tctgggtaac aagagaacct 360 tatgtgtcat gcagctccaa taaatgttat caatttgcac ttgggcaggg aaccacgcta 420 gataacagac actcaaacgg gacaatacat gatagaattc ctcatagaac cctcttaatg 480 aatgagttgg gtgttccgtt tcatttgggg accaaacagg tgtgtatagc atggtccagc 540 tcaagctgcc atgatgggaa agcatggtta cacgtttgtg ttactgggga tgatagaaat 600 gcaactgcta gtttcattta tgatggaacg cttgttgaca ctattggctc atggtcacaa 660 aatattctca gaactcagga gtcagaatgc gtttgcatca atggaacttg cactgtagta 720 atgactgatg gaagtgcatc aggaagagct gacactagag tactattcat taaagagggg 780 aaaattgtcc atattagcca attatcagga agtgctcagc acatagagga atgctcttgt 840 tatcccagat atccagatgt cagatgtgtt tacagagaca attggaaagg ctctaatagg 900 cccattatag atataaaaat ggcggattat agcattgatt ccagttatgt gtgctcggga 960 cttgttggcg atacaccaag ggatgatgat agctctagca acagcaactg taaagatcct 1020 aataatgaga gaggaaaccc aggagtgaaa gggtgggcct ttgactatgg aaatgatgtt 1080 tggatgggaa gaacaatcag caaggattca cgctcaggtt atgagacttt caaggtcatt 1140 gatggttgga ccacagctaa ctccaaatca caggtaaaca gacaagtcat agttgacaat 1200 aacaactggt ctggttatcc tggcattttc tctgttgaag gcaaaagctg cgtcaatagg 1260 tgtttttatg tggagttaat aagaggaagg ccacaagaga ctagagtatg gtggacctca 1320 aacagtattg tcgtgttttg tggcacttca ggtacatatg gaacaggctc atggcctgat 1380 ggggcgaata tcaattttat gcctata 1407 <210> 15 <211> 1027 <212> DNA <213> M gene of A/SW/KOREA/S109/04(H1N1) INFLUENZA VIRUS <400> 15 agcaaaagca ggtagatatt gaaagatgag tcttctaacc gaggtcgaaa cgtacgttct 60 ctctatcgtc ccgtcaggcc ccctcaaagc cgagatcgca cagagacttg aagatgtctt 120 tgcagggaag aacaccgatc ttgaggttct catggaatgg ctaaagacaa gaccaatcct 180 gtcacctctg actaagggga ttttaggatt tgtgttcacg ctcaccgtgc ccagtgagcg 240 gggactgcag cgtagacgct ttgtccaaaa tgctcttaat gggaacggag atccaaataa 300 catggacaaa gcagttaaac tgtataggaa gcttaagagg gagataacat tccatggggc 360 caaagaaata gcactcagtt attctgctgg tgcacttgcc tgttgtatgg gcctcatata 420 caacaggatg ggggctgtga ccactgaagc ggcatttggc ctggtatgcg caacctgtga 480 acagattgct gactcccagc atcggtctca taggcaaatg gtgacaacaa ccaatccact 540 aatcagacat gagaacagaa tggttctagc cagcactaca gctaaggcta tggagcaaat 600 ggctggatcg agtgagcaag cagcagaggc catggatatt gctagtcagg ccaggcaaat 660 ggtgcaggcg acgagaaccg ttgggactca tcctagctcc agtgctggtc taaaagatga 720 tcttcttgaa aatttacagg cctatcagaa acgaatgggg gtgcagatgc aacgattcaa 780 gtgatcctct cgtcattgca gcaaatatca ttggaatctt gcacttgata ttgtggattc 840 ttgatcgtct ttttttcaaa tgcatttatc gtcgttttaa atacggtttg aaaagagggc 900 cttctaccga aggagtgcca gagtctatga gggaagaata tcgaaaggaa cagcagaatg 960 ctgtggatgt tgacgatggt cattttgtca acatagagct ggagtaaaaa actaccttgt 1020 ttctact 1027 <210> 16 <211> 890 <212> DNA <213> NS gene of A/SW/KOREA/S109/04(H1N1) INFLUENZA VIRUS <400> 16 agcaaaagca gggtgacaaa gacataatgg atccaaacac tgtgtcaagc tttcaggtag 60 attgctttct ttggcatgtc cgcaaaagag ttgcagacca agaactaggt gatgccccat 120 tccttgatcg gcttcgccga gatcagaagt ccctaagagg aagaggcagc actcttggtc 180 tggacatcga aacagccacc cgtgctggaa agcaaatagt ggagcggatt ctgaaggaag 240 aatctgatga ggcactcaaa atgaccatgg cctctgtacc tgcatcgcgc tacctaactg 300 acatgactct tgaggaaatg tcaaggcact ggttcatgct catgcccaag cagaaagtgg 360 caggccctct ttgtatcaga atggaccagg cgatcatgga taagaacatc atactgaaag 420 cgaacttcag tgtgattttt gaccggctgg agactctaat attactaagg gccttcgccg 480 aagaggggac aattgttggc gaaatttcac cactgccctc tcttccagga catactgatg 540 aggatgtcaa aaatgcagtt ggggtcctca tcggaggact tgaatggaat aataacacag 600 ttcgagtctc tgaaactcta cagagattcg cttggagaag cagtaatgag aatgggagac 660 ctccactcac tccaaaacag aaacggaaaa tggcgggaac aattaggtca gaagtttgaa 720 gaaataaggt ggttgattga agaagtgaga cacagactga agataacaga gaatagtttt 780 gagcaaataa catttatgca agccttacaa ctattgcttg aagtggagca agagataaga 840 actttctcgt ttcagcttat ttaataataa aaaacaccct tgtttctact 890 <210> 17 <211> 1100 <212> DNA <213> PB2 gene of A/SW/KOREA/S81/04(H1N1) INFLUENZA VIRUS <400> 17 aatcaatcgc tgaagcgatc attgtagcaa tggtattttc acaagaggac tgcatgataa 60 aggcagtccg aggtgatttg aacttcatga acagagcgaa ccaacgactg aaccccatgc 120 accagcttct gaggcacttc caaaaagatg caaaagtgtt gtttcagaac tggggaattg 180 aacccatcga caatgtcatg ggaatgattg gaatattgcc tgacatgact cccagcacag 240 agatgtcact aagaggagtg agagtcagca agatgggagt ggacgagtac tccagcactg 300 aaagagtggt tgtgagtatc gatcgtttct tgagggttcg ggatcagagg gggaacatac 360 ttctatcccc tgaagaagtt agtgagacac aagggacgga aaagttaaca ataacatatt 420 catcgtctat gatgtgggag attaacggcc cggaatcagt gctagttaac acataccaat 480 ggatcattag gaattgggag actgtgaaga ttcagtggtc tcaagatcct actatgttat 540 acaataaggt ggaatttgaa cccttccaat ctctggtacc taaagctgcc agaggccaat 600 atagtggatt tgtgagaacg ctattccaac aaatgcgtga tgtactgggg acatttgaca 660 ctgttcaaat aataaagctg ctaccatttg ccgcggcccc accagagcag agtaggatgc 720 agttttcttc tctgactgtg aatgtaagag gctcaggaat gagaatcctt gtgagaggca 780 actcccctgt gtttaactac aacaaggcaa tcaagaggct tatagtcctc gggaaggatg 840 caggcgcact cacagaagac ccagatgagg gaacagcagg agtggaatcc gcagtattaa 900 gaggattcct aattctaggc aaagaagaca agagatacgg accagcattg agcatcaacg 960 aattgagcaa tcttgcgaaa ggagagaaag ccaatgtatt gatagggcaa ggagacgtag 1020 tgttggtaat gaaacggaaa cgggactctg gcatacttac tgacagccag acagcgacca 1080 aaagaattcg aatggccatc 1100 <210> 18 <211> 2341 <212> DNA <213> PB1 gene of A/SW/KOREA/S81/04(H1N1) INFLUENZA VIRUS <400> 18 agcgaaagca ggcaaaccat ttgaatggat gtcaatccga ctttactttt cttaaaagtg 60 ccagcgcaaa atgctataag cacaactttc ccttatactg gagaccctcc ttacagccat 120 gggacaggaa caggatacac catggatact gtcaacagga cacatcagta ctcagaaagg 180 ggaagatgga caacaaacac cgaaactgga gcaccgcaac tcaacccgat tgatgggcca 240 ctgccagaag acaatgaacc aagtggttat gcccaaacag atcgtgtatt ggaagcaatg 300 gccttccttg aggaatccca tcctggtatc tttgagacct cgtgtcttga aacgatggag 360 gttgttcagc aaacacgagt ggacaagctg acacaaggcc gacagaccta tgactggact 420 ctaaatagga accagcctgc tgcaacagca ttggccaaca caatagaagt gttcagatca 480 aatggcctca cggccaatga atctggaagg ctcatagact tccttaagga tgtaatggag 540 tcaatgaaca aagaagaaat ggagatcaca actcattttc agagaaagag acgagtgaga 600 gacaatatga ctaagaaaat ggtgacacag agaacaatag gtaaaaggaa gcagagattg 660 aacaaaggga gttatctaat tagggcatta accctgaaca caatgaccaa agatgctgag 720 agagggaagc taaaacggag agcaattgca accccaggga tgcaaataag ggggtttgta 780 tactttgttg agacactagc aaggagtata tgtgagaaac ttgaacaatc aggattgcca 840 gttggaggca atgagaagaa agcaaagttg gcaaatgttg taaggaagat gatgaccaat 900 tctcaggaca ctgaaatttc tttcaccatc actggagata acaccaaatg gaacgaaaat 960 cagaaccctc ggatgttttt ggccatgatc acatatataa ccagaaatca gcccgaatgg 1020 ttcagaaatg ttctaagtat tgctccaata atgttctcaa acaaaatggc gagactggga 1080 aaggggtaca tgtttgagag caagagtatg aaaattagaa ctcaaatacc tgcagaaatg 1140 ctagcaagca tcgatttgaa atacttcaat gattcaacta gaaagaagat tgaaaaaatc 1200 cggccgctct taatagatgg gactgcatca ttgagccctg gaatgatgat gggcatgttc 1260 aatatgttaa gtactgtatt aggcgtctcc atcctgaatc ttggacaaaa gagacacacc 1320 aagactactt actggtggga tggtcttcaa tcttctgatg attttgctct gattgtgaat 1380 gcacccaatc atgaagggat tcaagccgga gtcaacaggt tttatcgaac ctgtaagcta 1440 cttggaatta atatgagcaa gaaaaagtct tacataaaca gaacaggtac atttgaattc 1500 acaagttttt tctatcgtta tgggtttgtt gccaatttca gcatggagct tcccagcttt 1560 ggggtgtctg ggatcaacga gtctgcggac atgagtattg gagttactgt catcaaaaac 1620 aatatgataa acaatgatct tggtccagca accgctcaaa tggcccttca gctgttcatc 1680 aaagattaca ggtacacgta ccggtgccat agaggtgaca cacaaataca aacccgaaga 1740 tcatttgaaa taaagaaact gtgggagcaa acccattcca aagctggact gctggtctcc 1800 gacggaggcc caaatttata caacattaga aatctccaca ttcctgaagt ctgcttgaaa 1860 tgggaattaa tggatgagga ttaccagggg cgtttatgca acccactgaa cccatttgtc 1920 aaccataaag acattgaatc agtgaacaat gcagtgataa tgccagcaca tggtccagcc 1980 aaaaacatgg agtatgatgc tgttgcaaca acacactcct ggatccccaa aagaaatcga 2040 tccatcttga atacaagcca aagaggaata cttgaagatg aacaaatgta ccaaaagtgc 2100 tgcaacttat ttgaaaaatt cttccccagc agttcataca gaagaccagt cgggatatcc 2160 agtatggtgg aggctatggt ttccagagcc cgaattgatg cacgaattga tttcgaatct 2220 ggaaggataa agagagagga gttcactgag atcatgaaga tctgttccac cattgaagag 2280 ctcagacggc aaaaatagtg aatttagctt gtccttcatg aaaaaatgcc ttgtttctac 2340 t 2341 <210> 19 <211> 2209 <212> DNA <213> PA gene of A/SW/KOREA/S81/04(H1N1) INFLUENZA VIRUS <400> 19 atggaagatt ttgtgcgaca atggcttcaa tccgacattg tcgagcttgc ggaaaaggca 60 atgaaagagt atggagagga cctgaaaatc gaaacaaaca aatttgcagc aatatgcact 120 cacttggaag tgtgcttcat gtattcagat tttcacttca ccgatgagca aggcgagtca 180 atagtcgtag agcttggcga tccaaatgca cttttgaagc acagatttga aataatcgag 240 ggaagagatc gcacaatagc ctggacagta ataaacagta tttgcaacac tacaggggct 300 gagaaaccaa agtttctacc agatttgtat gattacaaga agaatagatt catcgaaatt 360 ggagtaacaa ggagagaagt tcacatatac tatctggaaa aggccaataa aattaaatct 420 gagaagacac acatccacat tttctcattc actggggagg aaatggccac aaaggccgac 480 tacactctcg atgaagaaag cagggctagg atcaaaacca ggctattcac cataagacaa 540 gaaatggcta gcagaggcct ctgggattcc tttcgtcagt ccgagagagg cgaagagaca 600 attgaagaaa gatttgaaat cacaggaaca atgcgcaagc ttgccgacca aagtctcccg 660 ccaaacttct ccagccttga aaaatttaga gcctatgtgg atggattcga accgaacggc 720 tacattgagg gcaagctttc tcaaatgtcc aaagaagtaa atgctagaat tgaacctttt 780 ttgaaatcaa caccacgacc acttagactt ccggatgggc ctccctgttc tcagcggtcc 840 aaattcctgc tgatggatgc cttaaaatta agcattgagg acccaagtca tgagggagag 900 gggataccgc tatatgatgc aatcaaatgc atgagaacat tctttggatg gaaggaaccc 960 aatgttgtta aaccacacga aaaggggata aatccaaatt atcttctgac atggaagcaa 1020 gtactggcag aactgcagga cattgagaat gaggagaaaa ttccaaggac taaaaatatg 1080 aagaaaacga gtcagttaaa gtgggcactt ggtgagaacg tggcactgga aaaggtagac 1140 tttgacgatt gtaaagatgt aggcgatttg aagcaatatg atagtgatga accagaattg 1200 aggtcgcttg caagttggat tcagaatgag ttcaacaagg catgtgaact gaccgattca 1260 agccggatag agctcgatga gattggagaa gatgcggctc caattgaaca cattgcaagc 1320 atgagaagga attatttcac agcagaggtg tctcattgca gagccacaga atacataatg 1380 aagggggtgt acatcaatac tgccgtgctt aatgcatcct gtgcagcaat ggatggtttc 1440 caattaattc caatgataag caagtgtaga actaaggagg gaaggcgaaa gaccaatttg 1500 tacggtttca tcataaaagg aagatcccac ttaaggaatg acaccgatgt ggtaaacttt 1560 gtgagcatgg agttttccct cactgaccca agacttgaac cacacaaatg ggagaagtac 1620 tgtgttcttg aggtaggaga tatgcttcta agaagtgcca taggccatgt gtcaaggcct 1680 atgttcttgt atgtgaggac aaatggaacc tcaaaaatta aaatgaaatg ggggatggaa 1740 atgaggcgtt gcctccttca gtcacttcaa caaatcgaga gtatgattga agctgagtcc 1800 tctgtcaagg agaaagacat gaccaaagag ttctttgaaa acaaatcaga aacatggccc 1860 gttggagagt cccccaaagg agtggaggaa ggttccattg ggaaggtctg cagaacttta 1920 ttggcaaagt cggtattcaa cagcttgtat gcatctccac aactagaagg attttcagct 1980 gaatcaagaa aactgcttct tatcgttcag gctcttaggg acaacctgga acctgggacc 2040 tttgatcttg gggggctata tgaagcaatt gaggagtgcc tgattaatga tccctgggtt 2100 ttgcttaatg cttcttggtt caactccttc ctcacgcatg cactgagata gttgtggcaa 2160 tgctactatt tgctatccat actgtccaaa aaagtacctt gtttctact 2209 <210> 20 <211> 1104 <212> DNA <213> HA gene of A/SW/KOREA/S81/04(H1N1) INFLUENZA VIRUS <400> 20 atggaagtaa tagcactaat agctatactg gtagtgacag gaacgagcaa tgctgataaa 60 atttgcattg gataccagtc aacaaactcc acagaaactg ttgatacact ggtagaaacc 120 aatgtccctg tgacacatgc caaagaactg cttcacacag agcacaatgg aatgttatgt 180 gcaacaaatc aggggcgtcc tcttatccta gacacctgca ccgttgaagg gttggtgtat 240 ggcaatcctt cctgtgattt gctactggga ggaaaagaat ggtcttacat tgtcgaaaga 300 tcatcagcca ttaatgggat gtgttaccct ggaagggtag agaatctgga agaactaaga 360 tcttttttca gttctgctcg ctcctacaaa agactcctgc tctttccaga ccgcacttgg 420 aatgtgactt acactggaac aagcaaagca tgctcaggtt cattctacag aagtatgaga 480 tggctgacac acaagagcaa ttcttaccct attcaagacg ctcaatatac caacgattgg 540 ggaaagaata ttctcttcat gtggggcata caccatccac ctactgatac tgagcaaatg 600 aatctataca aaaaagctga tacaacaacg agcataacaa cggaagatat caatcggact 660 ttcaaaccag tgataggacc aaggcccctt gtcaatggtc aacaagggag gattgattat 720 tattggtcag tactaaagcc aggccagaca ttgcgaataa gatccaatgg aaatctaatt 780 gctccatggt atggacacat tctttcagga gaaagccatg gaagaatcct aaagaccgat 840 ttaaatagtg gcaactgcgt agtacagtgc caaactgaga aaggtggttt gaatacgaca 900 ttgccattcc acaatgtcag caagtatgca tttgggaact gccccaaata tgttggagtg 960 aagagtctca agctggcagt tggcctaaga aatgtgcctg ctgcatcagg tagagggcta 1020 tttggtgcca tagctggatt catagaagga ggttggccag ggctagttgc aggctggtac 1080 gggtttcagc attcaaatga tcaa 1104 <210> 21 <211> 543 <212> DNA <213> NP gene of A/SW/KOREA/S81/04(H1N1) INFLUENZA VIRUS <400> 21 ggagactgat ggagaacgcc agaatgccac tgaaatcaga gcatctgtcg gaaaaatgat 60 tgatggaatt ggacgattct acatccaaat gtgcaccgaa cttaaactca gtgattatga 120 gggacggctg attcagaaca gcttaacaat agagagaatg gtgctctctg cttttgacga 180 gaggaggaat aaatatctag aagaacatcc cagtgcgggg aaagatccta agaaaactgg 240 aggacctata tacaggagag tagatggaaa gtggaggaga gaactcatcc tttatgacaa 300 agaagaaata agacgaatct ggcgccaagc taataatggt gacgatgcaa cggctggtct 360 gactcacatg atgatctggc actccaattt gaatgatgca acttaccaga ggacaaaagc 420 tcttgttcgc acaggaatgg atcccaggat gtgctcactg atgcagggtt caaccctccc 480 taggaggtct ggggccgcag gtgctgcagt caaaggagtt ggaacaatgg tgatggaatt 540 gat 543 <210> 22 <211> 1410 <212> DNA <213> NA gene of A/SW/KOREA/S81/04(H1N1) INFLUENZA VIRUS <400> 22 atgaatccaa atcagaaaat aataacaatt ggctctgtct ctctaaccat tgcaacagta 60 tgtttcctca tgcagattgc cattctagca acaactgtaa cactgcactt caagcaaaat 120 gaatgcagca taccctcgaa caaccaagca gttccatgtg aaccaatcat aatagaaaag 180 aacataacaa aaatagtgta tttgaataat actaccatag agaaagaact ttgtcctaaa 240 gtggtagaat acaggagttg gtcgaaaccg cagtgtcaga ttacagggtt tgctcctttc 300 tccaaggaca actcagtccg gctctcggct ggtgggagca tttgggtaac aagagaacct 360 tacgtgtcat gcagttccaa taaatgttat caatttgcac tcgggcaggg aaccacgcta 420 gataacaaac actcaaatgg gacaatacat gatcgaatcc ctcatcgaac ccttttaatg 480 aacgaattgg gtgttccgtt tcatttggga accaaacagg tgtgtatagc atggtccagc 540 tcaagctgcc atgatgggaa agcatggtta catgtttgtg tcactggaga tgatagaaat 600 gcaactgcta gtttcattta tgatggaatg cttgttgaca gtattggctc atggtctcaa 660 aatattctca gaactcagga gtcagaatgc gtttgcatca atggaacttg cactgtagta 720 atgactgatg gaagtgcatc aggaagagct gacactagag tactattcat taaagagggg 780 aaaattgtcc atattagcca attatcagga agtgctcagc acatagagga atgctcttgt 840 tatcccagat atccagatgt cagatgtgtt tacagagaca attggaaagg ctctaatagg 900 cccattatag atataaaaat ggcggattat agcattgatt ccagttatgt gtgctcggga 960 cttgttggcg atacaccaag ggatgatgat agctctagca acagcaactg taaagatcct 1020 aataatgaga gaggaaaccc aggagtgaaa gggtgggcct ttgactatgg aaatgatgtt 1080 tggatgggaa gaacaatcag caaggattca cgctcaggtt atgagacttt caaggtcatt 1140 gatggttgga ccacagctaa ctccaaatca caggtaaaca gacaagtcat agttggcaat 1200 aacaactggt ctggttattc tggcattttc tctgttgaag gcaaaagctg cgtcaatagg 1260 tgtttttatg tggagttaat aagaggaagg ccacaagaga ctagagtatg gtggacctca 1320 aacagtattg tcgtgttttg tggcacttca ggtacatatg gaacaggctc atggcctgat 1380 ggggcgaata tcaattttat gcctatataa 1410 <210> 23 <211> 1027 <212> DNA <213> M gene of A/SW/KOREA/S81/04(H1N1) INFLUENZA VIRUS <400> 23 agcaaaagca ggtagatatt gaaagatgag tcttctaacc gaggtcgaaa cgtacgttct 60 ctctatcgtc ccgtcaggcc ccctcaaagc cgagatcgca cagagacttg aagatgtctt 120 tgcagggaag aacaccgatc ttgaggttct catggaatgg ctaaagacaa gaccaatcct 180 gtcacctctg actaagggga ttttaggatt tgtgttcacg ctcaccgtgc ccagtgagcg 240 gggactgcag tgtagacgct ttgtccaaaa tgctcttaat gggaacggag atccaaataa 300 catggacaaa gcagttaaac tgtataggaa gcttaagagg gagataacat tccatggggc 360 caaagaaata gcactcagtt attctgctgg tgcacttgcc tgttgtatgg gcctcatata 420 caacaggatg ggggctgtga ccactgaagt ggcatttggc ctggtatgcg caacctgtga 480 acagattgct gactcccagc atcggtctca taggcaaatg gtgacaacaa ccaatccact 540 aatcagacat gagaacagaa tggttctagc cagcactaca gctaaggcta tggagcaaat 600 ggctggatcg agtgagcaag cagcagaggc catggatatt gctagtcagg ccaggcaaat 660 ggtgcaggcg atgagaaccg ttgggactca tcctagctcc agtgctggtc taaaagatga 720 tcttcttgaa aatttacagg cctatcagaa acgaatgggg gtgcagatgc aacgattcaa 780 gtgatcctct cgtcattgca gcaaatatca ttggaatctt gcacttgata ttgtggattc 840 ttgatcgtct ttttttcaaa tgcatttatc gtcgctttaa atacggtttg aaaagagggc 900 cttctaccga aggagtgcca gagtctatga gggaagaata tcgaaaggaa cagcagaatg 960 ctgtggatgt tgacgatggt cattttgtca acatagagct ggagtaaaaa actaccttgt 1020 ttctact 1027 <210> 24 <211> 891 <212> DNA <213> NS gene of A/SW/KOREA/S81/04(H1N1) INFLUENZA VIRUS <400> 24 agcaaaagca gaggtgacaa aaacatgatg gattccaata ctgtgtcaag ctttcaggta 60 gactgctttc tttggcatgt ccgcaaacga tttgcagacc aagaactggg tgatgcccca 120 ttccttgacc ggcttcgccg agatcagaag tccctaagag gaagaggcaa cactcttggt 180 ctggacatcg aaacagctac ccatgcggga aaacggatag tagagcggat tctggaagaa 240 gaacccgatg aggcacttaa aatggctgtt gcttcagtcc ctgcttcacg ctatctagct 300 gatatgactc ttgaagaaat gtcaagggac tggtttatgc tcatgcccaa gcagaaagtg 360 gctgggtccc tttgtatcaa aatggaccag gcaataatgg ataaaaatat cacattaaaa 420 gcaaacttca gtgtgatttt taatcggcta gaaaccctaa tactactcag agctttcaca 480 gaagaaggag taattgtggg ggaaatctca ccgttacctt ctcttccagg acatactaat 540 gaggatgtca aaaatgcaat tggggccctc atcggaggac ttgagtggaa tgataacaca 600 gttcgagtct ctgaaactct acagaggctc acttggagaa gcagtgatga gaatggggga 660 ccttcactcc ctccaaaaca gaaacggaaa atggcgagaa caattgagtc agaaatttga 720 agaaataaga tggctgatcg aagaagtgcg acatagatta aagataaccg agaacagctt 780 tgaacagata acatttatgc aagccttgca actattgctt gaagtagagc aagagataag 840 aaccttctcg tttcagctta tttaataata aaaaacaccc ttgtttctac t 891 <210> 25 <211> 1036 <212> DNA <213> PB2 gene of A/SW/KOREA/S83/04(H1N1) INFLUENZA VIRUS <400> 25 agtccgaggt gatttgaact tcgtgaacag agcgaaccaa cgactgaacc ccatacacca 60 gcttctgagg cacttccaaa aagatgcaaa agtgttgttt cagaactggg gaattgaacc 120 catcgacaat gtcatgggga tgattggggt attgcctgac atgactccca gcacagagat 180 gtcactaaga ggagtgagag tcagcaagat gggagtggac gagtactcca gcactgaaag 240 agtggttgtg agtattgatc gtttcttgag ggttcgggat cagaggggga acatacttct 300 atcccctgaa gaagttagtg agacacaagg gacggaaaag ttaacaataa catattcatc 360 gtctatgatg tgggagatta acggcccgga atcagtgcta gttaacacat accaatggat 420 cattaggaat tgggagactg tgaagattca gtggtctcaa gatcctacta tgttatacaa 480 taaggtggaa tttgaaccct tccaatctct ggtacctaaa gctgccagag gccaatatag 540 tggatttgtg agaacgctat tccaacaaat gcgtgatgta ctggggacat ttgacactgt 600 tcaaataata aagctgctac catttgccgc agccccacca gagcagagta ggatgcagtt 660 ttcttctctg actgtgaatg caagaggctc gggaatgaga atccttgtga gaggcaactc 720 ccctgtgttt aactacaaca aggcaatcaa gaggcttaca gtcctcggga aggatgcagg 780 cgcactcaca gaagacccag atgagggaac agcaggagtg gaaaccgcag tattaagagg 840 attcctaatt ctaggcaaag aagacaagag atacggacca gcattgagca tcaacgaatt 900 gagcaatctt gcgaaaggag agaaagccaa tgtattgata gggcaaggag acgtagtgtt 960 ggtaatgaaa cggaaacggg actctagtat acttactgac agccagacag cgatcgaaag 1020 gattcgaatg gccatc 1036 <210> 26 <211> 1178 <212> DNA <213> PB1 gene of A/SW/KOREA/S83/04(H1N1) INFLUENZA VIRUS <400> 26 gattcaacta gaaagaagat tgaaaaaatc cggccgctct taatagatgg gactgcatca 60 ttgagccctg gaatgatgat gggcatgttc aatatgttaa gtactgtatt aggcgtctcc 120 atcctgaatc ttggacaaaa gagacacacc aagactactt actggtggga cggtcttcaa 180 tcttctgatg attttgctct gattgtgaat gcacccaatc atgaagggat tcaagccgga 240 gtcaacaggt tttatcgaac ctgcaagcta cttggaatta atatgagcaa gaaaaagtct 300 cacataaaca gaacaggtac atttgaattc acaagttttt tctatcgtta tgggtttgtt 360 gccaatttca gcatggagct tcccagcttt ggggtgtctg ggatcaacga gtctgcggac 420 gcgagtattg gagttactgt catcaaaaac aatatgataa acaatgatct tggtccagca 480 accgctcaaa tggcccttca gctgttcatc aaagattaca ggtacacgta ccggtgccat 540 agaggtgaca cacaaataca aacccgaaga tcatttgaaa taaagaaact gtgggagcaa 600 acccattcca aagctggact gctggtctcc gacggaggcc caaatttata caacattaga 660 aatctccaca ttcctgaagt ctgcttgaaa tgggaattaa tggatgagga ttaccagggg 720 cgtttatgca acccactgaa cccatttgtc aaccataaag acattgaatc agtgaacaat 780 gcagtgatag tgccagcaca tggtccagcc aaaaacatgg agtatgatgc tgttgcaaca 840 acacactcct ggatccccaa aagaaatcga tccatcttga atacaagcca aagaggaata 900 cttgaagatg aacaaatgta ccaaaagtgc tgcaacttat ttgaaaaatt cttccccagc 960 agttcataca gaagaccagt cgggatatcc agtatggtgg aggctatggt ttccagagcc 1020 cgaattgatg cacgaattga tttcgaatct ggaaggataa agaaagagga gttcactgag 1080 atcatgaaga tctgttccac cattgaagag ctcagacggc aaaaatagtg aatttagctt 1140 gtccttcatg aaaaaatgcc ttgtttctac taatcact 1178 <210> 27 <211> 2210 <212> DNA <213> PA gene of A/SW/KOREA/S83/04(H1N1) INFLUENZA VIRUS <400> 27 atggaagatt ttgtgcgaca atgcttcaat ccgatgattg tcgagcttgc ggaaaaggca 60 atgaaagagt atggagagga cctgaaaatc gaaacaaaca aatttgcagc aatatgcact 120 cacttggaag tgtgcttcat gtattcagat tttcacttca tcgatgagca aggcgagtca 180 atagtcgtag aacttggcga tccaaatgca cttttgaagc acagatttga aataatcgag 240 ggaagagatc gcacaatagc ctggacagta ataaacagta tttgcaacac tacaggggct 300 gagaaaccaa agtttctacc agatttgtat gattacaaga agaatagatt catcgaaatt 360 ggagtaacaa ggagagaagt tcacatatac tatctggaaa aggccaataa aattaaatct 420 gagaagacac acatccacat tttctcattc actggggagg aaatggccac aaaggccgac 480 tacactctcg atgaagaaag cagggctagg atcaaaacca ggctattcac cataagacaa 540 gaaatggcta gcagaggcct ctgggattcc tttcgtcagt ccgagagagg cgaagaggca 600 attgaagaaa gatttgaaat cacaggaaca atgcgcaagc ttgccgacca gagtcttccg 660 ccaaacttct ccagccttga aaaatttaga gcctatgtgg atggattcga accgaacggc 720 tacattgagg gcaagctttc tcaaatgtcc aaagaagtaa atgctagaat tgaacctttt 780 ttgaaatcaa caccacgacc acttagactt ccggatgggc ctccctgttc tcagcggtcc 840 aaattcctgc ggatggatgc cttaaaatta agcattgagg acccaagtca tgagggagag 900 gggataccgc tatatgatgc aatcaaatgc atgagaacat tctttggatg gaaggaaccc 960 aatgttgtta aaccacacga aaagggaata aatccaaatt atcttctgtc atggaagcaa 1020 gtactggcag aactgcagga cattgagaat gaggagaaaa ttccaaggac taaaaatatg 1080 aagaaaacga gtcagttaaa gtgggcactc ggtgagaaca tggcaccaga aaaggtagac 1140 tttgacggtt gtaaagatat aggcgatttg aagcaatatg atagtgatga accagaattg 1200 aggtcgcttg caagttggat tcagaatgag ttcaacaagg catgtgaact gaccgattca 1260 agctggatag agctcgatga gattggagaa gatgcggctc caattgaaca cattgcaagc 1320 atgagaagga attatttcac agcagaggtg tctcattgca gagccacaga atacataatg 1380 aagggggtgt acatcaatac tgccttgctt aatgcatcct gtgcagcaat ggatgatttc 1440 caattaattc caatgataag caagtgtaga actaaggagg gaaggcgaaa gaccaatttg 1500 tacggtttca tcataaaagg aagatcccac ttaaggaatg acaccgatgt ggtaaacttt 1560 gtgagcatgg agttttccct cactgaccca agacttgaac cacacaaatg ggagaagtac 1620 tgtgttcttg aggtaggaga tatgcttcta agaagtgcca taggccatgt gtcaaggcct 1680 atgttcttgt atgtgaggac aaatggaacc tcaaaaatta aaatgaaatg ggggatggaa 1740 atgaggcgtt gcctccttca gtcacttcaa caaatcgaga gtatgattga agctgagtcc 1800 tctgtcaagg agaaagacat gaccaaagag ttctttgaaa acaaatcaga aacatggccc 1860 gttggagagt cccccaaagg agtggaggaa ggttccattg ggaaggtctg cagaacttta 1920 ttggcaaagt cggtattcaa cagcttgtat gcatctccac aactagaagg attttcagct 1980 gaatcaagaa aactgcttct tatcgttcag gctcttaggg acaacctgga acctgggacc 2040 tttgatcttg gggggctata tgaagcaatt gaggagtgcc tgattaatga tccctgggtt 2100 ttgcttaatg cttcttggtt caactccttt ctcacacatg cattgagata gttgtggcaa 2160 tgctactatt tgctatccat actgtccaaa aaagtaccct tgtttctact 2210 <210> 28 <211> 1104 <212> DNA <213> HA gene of A/SW/KOREA/S83/04(H1N1) INFLUENZA VIRUS <400> 28 atggaagtaa tagcactaat agctatactg gtagtgacag gaacgagcag tgctgataaa 60 atttgcattg gataccagtc aacaaactcc acagaaactg ttgatacact agtagaaacc 120 aatgtccctg tgacacatgc caaagaactg cttcacacag agcacaatgg aatgttatgt 180 gcaacaaatc aggggcgtcc tcttatccta gacacctgca ccgttgaagg gttggtgtat 240 ggcaatcctt cccgtgattt gctactggga ggaaaagaat ggtcttacat tgtcaaaaga 300 tcatcagcca ttaatgggat gtgttaccct ggaagggtag agaatctgga agaactaaga 360 tcttttttca gttctgctcg ctcctacaaa agactcctgc tctttccaga ccgcacttgg 420 aatgtgactt acactggaac aagcaaagca tgctcaggtt cattctacag aagtatgaga 480 tggctgacac acaagagcaa ttcttaccct attcaagacg ctcaatatac caacgattgg 540 ggaaagaata ttctcttcat gtggggcata caccatccac ctactgatac tgagcaaatg 600 aatctataca aaaaagctga tacaacaacg agcataacaa cggaagatat caatcggact 660 ttcaaaccag tgataggacc aaggcccctt gtcaatggtc aacaagggag gattgattat 720 tattggtcag tactaaagcc aggccagaca ttgcgaataa gatccaatgg aaatctaatt 780 gctccatggt atggacacat tccttcagga gaaagccatg gaagaatcct aaagaccgat 840 ttaaatagtg gcaactgcgt agtacagtgc caaactgaga aaggtggttt gaatacgaca 900 ttgccattcc acaatgtcag caagtatgca tttgggaact gccccaaata tgttggagtg 960 aagagtctca agctggcagt tggtctaaga aatgtgcctg ctgcatcagg tagagggcta 1020 tttggtgcca tagctggatt catagaagga ggttggccag ggctagttgc aggctggtac 1080 gggtttcagc attcaaatga tcaa 1104 <210> 29 <211> 543 <212> DNA <213> NP gene of A/SW/KOREA/S83/04(H1N1) INFLUENZA VIRUS <400> 29 ggagactgat ggagaacgcc agaatgccac tgaaatcaga gcatctgtcg gaaaaatgat 60 tgatggaatt ggacgattct acatccaaat gtgcaccgaa cttaaactca gtgattatga 120 gggacggctg attcagaaca gcttaacaat agagagaatg gtgctctctg cttttgacga 180 gaggaggaat aaatatctag aagaacatcc cagtgcgggg aaagatccta agaaaactgg 240 aggacctata tacaggagag tagatggaaa gtggaggaga gaactcatcc tttatgacaa 300 agaagaaata agacgaatct ggcgccaagc taataatggt gacgatgcaa cggctggtct 360 gactcacatg atgatctggc actccaattt gaatgatgca acttaccaga ggacaagagc 420 tcttgttcgc acaggaatgg atcccaggat gtgctcactg atgcagggtt caaccctccc 480 taggaggtct ggggccgcag gtgctgcagt caaaggagtt ggaacaatgg tgatggaatt 540 gat 543 <210> 30 <211> 1410 <212> DNA <213> NA gene of A/SW/KOREA/S83/04(H1N1) INFLUENZA VIRUS <400> 30 atgaatccaa atcagaaaat aataacaatt ggctctgtct ctctaaccat tgccacagta 60 tgtttcctca tgcagattgc cattctagca acgactgtaa cactgcactt caagcaaaat 120 gaaggcagca ttccctcgaa caatcaagta gttccatgtg aaccaatcat aatagaaagg 180 aacataacag aaatagtgta tttaaataat actatcatag agaaagaact ttgccctaaa 240 gtggtaaaat ataggagttg gtcgaaaccg cagtgtcaga tcacagggtt tgctcctttc 300 tccaaggacg actcaatccg gctgtctgct ggtgggagca tctgggtaac aagagaacct 360 tatgtgtcat gcagctccaa taaatgttat caatttgcac ttgggcaggg aaccacgcta 420 gataacagac actcaaacgg gacaatacat gatagaattc ctcatagaac cctcttaatg 480 aatgagttgg gtgttccgtt tcatttgggg accaaacagg tgtgtatagc atggtccagc 540 tcaagctgcc atgatgggaa agcatggtta catgtttgtg ttactgggga tgatagaaat 600 gcaactgcta gtttcattta tgatggaacg cttgttgaca ctattggctc atggtcacag 660 aatattctca gaacccagga gtcagaatgc gtttgcatca atggaacttg cacagtagta 720 atgaccgatg gaagtgcgtc agggagagct gacactagaa tactattcat taaagagggg 780 aaaattgtcc atattagcca attatcagga agtgctcagc acatagagga atgctcttgt 840 tatcccagat atccagatgt cagatgtgtt tgcagagaca attggaaagg ctctaatagg 900 cccattatag atataaaaat ggcggattat agcattgatt ccagttatgt gtgctcgggg 960 cttgttggcg atacaccaag gaatgatgat agctctagca acagcaactg taaagatcct 1020 aataatgaga gaggaaaccc aggagtgaaa gggtgggcct ttgactatgg aaatgatgtt 1080 tggatgggaa gaacaatcag caaggattca cgctcaggtt atgagacttt caaggtcatt 1140 gatggttgga ccacagctaa ctccaaatca caggtgaaca gacaagtcat agttgacaat 1200 aacaactggt ctggttgttc tggcattttc tctgttgaag gcaaaagctg cgtcaatagg 1260 tgtttttatg tggagttaat aagaggaagg ccacaagaga ctagagtatg gtggacctca 1320 aacagtattg tcgtgttttg tggcacttca ggtacatatg gaacaggctc atggcctgag 1380 ggggcgaata tcaattttat gcctatataa 1410 <210> 31 <211> 1011 <212> DNA <213> M gene of A/SW/KOREA/S83/04(H1N1) INFLUENZA VIRUS <400> 31 tagatattga aagatgagtc ttctgaccga ggtcgaaacg tacgttctct ctatcatccc 60 gtcaggcccc ctcaaagccg agatcgcgca gggacttgaa gatgtctttg cagggaagaa 120 cacagatctc gaggcactaa tggaatggct aaagacaaga ccaatcctgt cacctctgac 180 taaggggatt ttaggatttg tgttcacgct caccgtgccc ggtgagcgag gacttcagcg 240 tagacgcttt gtccaaaatg ccctaaacgg gaacggggac ccaaacaaca tggacagagc 300 agtcaaactg tacagaaagc tgaaacgaga aataacattt catggagcca aagaggttgc 360 tcttagctac tcagccggtg cacttgccag ttgcatgggt ctcatataca acaggatggg 420 aacagtaacc acagaagtgg cttttggcct agtatgtgct acttgcgagc agattgctga 480 ctcgcagcat cggtctcaca gacagatggt gactactacc aatccactaa tcaggcatga 540 aaatagaatg gtactggcca gcacaacggc taaggctatg gaacagatgg ctggatcaag 600 cgagcaagcg gcagaggcta tggaggttgc tagtcaggct aggcaaatgg tgcaagcgat 660 gagaacaatt gggacccatc ctagctccag tgccggtctg aaagatgatc ttcttgaaaa 720 tttgcaggcc taccagaaaa ggatgggagt gcaaatgcag cgattcaagt gatcctctcg 780 ttattgccgc aagtatcatt gggatcttgc acttgatatt gtggattctt gatcgtcttt 840 tcttcaaatg catttatcgt cgccttaaat acggtttgaa aagagggcct tctacggaag 900 ggatgcctga gtctatgagg gaagagtatc ggcaggagca gcagagtgct gtggatgttg 960 acgatggtca ttttgtcaac atagagctgg agtaaaaaac taccttgttt c 1011 <210> 32 <211> 835 <212> DNA <213> NS gene of A/SW/KOREA/S83/04(H1N1) INFLUENZA VIRUS <400> 32 aagcagggtg acaaaaacat gatggattcc aatactgtgt caagctttca agtagactgc 60 tttctttggc atgtccgcaa acgatttgca gaccaagaac tgggtgatgc cccattcctt 120 gaccggcttc gccgagatca gaagtcccta agaggaagag gcaacactct tggtctggac 180 atcgaaacag ctacccatgc gggaaaacgg atagtagagc ggattctgga agaagaaccc 240 gatgaggcac ttaaaatggc tgttgcttca gtccctgctt cacactatct agctgatatg 300 actcttgaag aaatgtcaag ggactggttt atgctcatgc ccaagcagaa agtggctggg 360 tccctttgta tcaagatgga ccaggcaata atggataaaa atatcacatt aaaagcaaac 420 ttcagtgtga tttttaatcg gctagaaacc ctaatactac tcagagcttt cacagaagaa 480 ggagtaattg tgggggaaat ctcaccgtta ccttctcttc caggacatac taatgaggat 540 gtcaaaaatg caattggggc cctcatcgga ggacttgagt ggaatgataa cacagttcga 600 gtctctgaaa ctctacagag gctcacctgg agaagcagtg atgagaatgg gggaccttca 660 ctccctccaa aacagaaacg gaaaatggcg agaacaattg agtcagaaat ttgaagaaat 720 aagatggctg atcgaagaag tgcgacatag attaaagata accgagaaca gctttgaaca 780 gataacattt atgcaagccc ttgcaactat tgcttgaagt agagcaagag ataag 835 <210> 33 <211> 755 <212> PRT <213> PB2 of A/swine/Korea/S10/2004(H1N1) <400> 33 Ile Lys Glu Leu Arg Asp Leu Met Ser Gln Ser Arg Thr Arg Glu Ile 1 5 10 15 Leu Thr Lys Thr Thr Val Asp His Met Ala Ile Ile Lys Lys Tyr Thr 20 25 30 Ser Gly Arg Gln Glu Lys Asn Pro Ala Leu Arg Met Lys Trp Met Met 35 40 45 Ala Met Lys Tyr Pro Ile Thr Ala Asp Lys Arg Ile Met Glu Met Ile 50 55 60 Pro Glu Arg Asn Glu Gln Gly Gln Thr Leu Trp Ser Lys Thr Asn Asp 65 70 75 80 Ala Gly Ser Asp Lys Val Met Val Ser Pro Leu Ala Val Thr Trp Trp 85 90 95 Asn Arg Asn Gly Pro Thr Thr Ser Thr Ile His Tyr Pro Lys Val Tyr 100 105 110 Lys Thr Tyr Phe Glu Lys Val Glu Arg Leu Lys His Gly Thr Phe Gly 115 120 125 Pro Ile His Phe Arg Asn Gln Val Lys Ile Arg Arg Thr Val Asp Thr 130 135 140 Asn Pro Gly His Ala Asp Leu Ser Ala Lys Glu Ala Gln Asp Val Ile 145 150 155 160 Met Glu Val Val Phe Pro Asn Glu Val Gly Ala Arg Ile Leu Thr Ser 165 170 175 Glu Ser Gln Leu Thr Ile Thr Lys Glu Lys Lys Glu Glu Leu Gln Asp 180 185 190 Cys Lys Ile Ala Pro Leu Met Val Ala Tyr Met Leu Glu Arg Glu Leu 195 200 205 Val Arg Lys Thr Arg Phe Leu Pro Val Ala Gly Gly Thr Ser Ser Val 210 215 220 Tyr Ile Glu Val Leu His Leu Thr Gln Gly Thr Cys Trp Glu Gln Met 225 230 235 240 Tyr Thr Pro Gly Gly Glu Val Arg Asn Asp Asp Val Asp Gln Ser Leu 245 250 255 Ile Ile Ala Ala Arg Asn Ile Val Arg Arg Ala Thr Val Ser Ala Asp 260 265 270 Pro Leu Ala Ser Leu Leu Glu Met Cys His Gly Thr Gln Ile Gly Gly 275 280 285 Ile Arg Met Val Asp Ile Leu Arg Gln Asn Pro Thr Glu Glu Gln Ala 290 295 300 Val Asp Ile Cys Lys Ala Ala Met Gly Leu Arg Ile Ser Ser Ser Phe 305 310 315 320 Ser Phe Gly Gly Phe Thr Phe Lys Arg Thr Ser Gly Ser Ser Val Lys 325 330 335 Lys Glu Glu Glu Val Leu Thr Gly Asn Leu Gln Thr Leu Lys Ile Arg 340 345 350 Val His Glu Gly Tyr Glu Glu Phe Thr Met Val Gly Arg Arg Ala Thr 355 360 365 Ala Leu Leu Arg Lys Ala Thr Arg Arg Leu Ile Gln Leu Ile Val Ser 370 375 380 Gly Arg Asp Glu Gln Ser Ile Ala Glu Ala Ile Ile Val Ala Met Val 385 390 395 400 Phe Ser Gln Glu Asp Cys Met Ile Lys Ala Val Arg Gly Asp Leu Asn 405 410 415 Phe Met Asn Arg Ala Asn Gln Arg Leu Asn Pro Met His Gln Leu Leu 420 425 430 Arg His Phe Gln Lys Asp Ala Lys Val Leu Phe Gln Asn Trp Gly Ile 435 440 445 Glu Pro Ile Asp Asn Val Met Gly Met Ile Gly Ile Leu Pro Asp Met 450 455 460 Thr Pro Ser Thr Glu Met Ser Leu Arg Gly Val Arg Val Ser Lys Met 465 470 475 480 Gly Val Asp Glu Tyr Ser Ser Thr Glu Arg Val Val Val Ser Ile Asp 485 490 495 Arg Phe Leu Arg Val Arg Asp Gln Arg Gly Asn Ile Leu Leu Ser Pro 500 505 510 Glu Glu Val Ser Glu Thr Gln Gly Thr Glu Lys Leu Thr Ile Thr Tyr 515 520 525 Ser Ser Ser Met Met Trp Glu Ile Asn Gly Pro Glu Ser Val Leu Val 530 535 540 Asn Thr Tyr Gln Trp Ile Ile Arg Asn Trp Glu Thr Val Lys Ile Gln 545 550 555 560 Trp Ser Gln Asp Pro Thr Met Leu Tyr Asn Lys Val Glu Phe Glu Pro 565 570 575 Phe Gln Ser Leu Val Pro Lys Ala Ala Arg Gly Gln Tyr Ser Gly Phe 580 585 590 Val Arg Thr Leu Phe Gln Gln Ile Arg Asp Val Leu Gly Thr Phe Asp 595 600 605 Thr Val Gln Ile Ile Lys Leu Leu Pro Phe Ala Ala Ala Pro Pro Glu 610 615 620 Gln Ser Arg Met Gln Phe Ser Ser Leu Thr Val Asn Val Arg Gly Ser 625 630 635 640 Gly Met Arg Ile Leu Val Arg Gly Asn Ser Pro Val Phe Asn Tyr Asn 645 650 655 Lys Ala Thr Lys Arg Leu Thr Val Leu Gly Lys Asp Ala Gly Ala Leu 660 665 670 Thr Glu Asp Pro Asp Glu Gly Thr Ala Gly Val Glu Ser Ala Val Leu 675 680 685 Arg Gly Phe Leu Ile Leu Gly Lys Glu Asp Lys Arg Tyr Gly Pro Ala 690 695 700 Leu Ser Ile Asn Glu Leu Ser Asn Leu Ala Lys Gly Glu Lys Ala Asn 705 710 715 720 Val Leu Ile Gly Gln Gly Asp Val Val Leu Val Met Lys Arg Lys Arg 725 730 735 Asp Ser Ser Ile Leu Thr Asp Ser Gln Thr Ala Thr Lys Arg Ile Arg 740 745 750 Met Ala Ile 755 <210> 34 <211> 757 <212> PRT <213> PB1 of A/swine/Korea/S10/2004(H1N1) <400> 34 Met Asp Val Asn Pro Thr Leu Leu Phe Leu Lys Val Pro Ala Gln Asn 1 5 10 15 Ala Ile Ser Thr Thr Phe Pro Tyr Thr Gly Asp Pro Pro Tyr Ser His 20 25 30 Gly Thr Gly Thr Gly Tyr Thr Met Asp Thr Val Asn Arg Thr His Gln 35 40 45 Tyr Ser Glu Arg Gly Arg Trp Thr Thr Asn Thr Glu Thr Gly Ala Pro 50 55 60 Gln Leu Asn Pro Ile Asp Gly Pro Leu Pro Glu Asp Asn Glu Pro Ser 65 70 75 80 Gly Tyr Ala Gln Thr Asp Cys Val Leu Glu Ala Met Ala Phe Leu Glu 85 90 95 Glu Ser His Pro Gly Ile Phe Glu Thr Ser Cys Leu Glu Thr Met Glu 100 105 110 Val Val Gln Gln Thr Arg Val Asp Lys Leu Thr Gln Gly Arg Gln Thr 115 120 125 Tyr Asp Trp Thr Leu Asn Arg Asn Gln Pro Ala Ala Thr Ala Leu Ala 130 135 140 Asn Thr Ile Glu Val Phe Arg Ser Asn Gly Leu Thr Ala Asn Glu Pro 145 150 155 160 Gly Arg Leu Ile Asp Phe Leu Lys Asp Val Val Glu Ser Met Asn Lys 165 170 175 Glu Glu Met Glu Ile Thr Thr His Phe Gln Arg Lys Arg Arg Val Arg 180 185 190 Asp Asn Met Thr Lys Lys Met Val Thr Gln Arg Thr Ile Gly Lys Arg 195 200 205 Lys Gln Arg Leu Asn Lys Arg Ser Tyr Leu Ile Arg Ala Leu Thr Leu 210 215 220 Asn Thr Met Thr Lys Asp Ala Glu Arg Gly Lys Leu Lys Arg Arg Ala 225 230 235 240 Ile Ala Thr Pro Gly Met Gln Ile Arg Gly Phe Val Tyr Phe Val Glu 245 250 255 Thr Leu Ala Arg Ser Ile Cys Glu Lys Leu Glu Gln Ser Gly Leu Pro 260 265 270 Val Gly Gly Asn Glu Lys Lys Ala Lys Leu Ala Asn Val Val Arg Lys 275 280 285 Met Met Thr Asn Ser Gln Asp Thr Glu Ile Ser Phe Thr Ile Thr Gly 290 295 300 Asp Asn Thr Lys Trp Asn Glu Asn Gln Asn Pro Arg Met Phe Leu Ala 305 310 315 320 Met Ile Thr Tyr Ile Thr Arg Asn Gln Pro Glu Trp Phe Arg Asn Val 325 330 335 Leu Ser Ile Ala Pro Ile Met Phe Ser Asn Lys Met Ala Arg Leu Gly 340 345 350 Lys Gly Tyr Met Phe Glu Ser Lys Ser Met Lys Ile Arg Thr Gln Ile 355 360 365 Pro Ala Glu Met Leu Ala Ser Ile Asp Leu Lys Tyr Phe Asn Asp Ser 370 375 380 Thr Arg Lys Lys Ile Glu Lys Ile Arg Pro Leu Leu Ile Asp Gly Thr 385 390 395 400 Ala Ser Leu Ser Pro Gly Met Met Met Gly Met Phe Asn Met Leu Ser 405 410 415 Thr Val Leu Gly Val Ser Ile Leu Asn Leu Gly Gln Lys Arg His Thr 420 425 430 Lys Thr Thr Tyr Trp Trp Asp Gly Leu Gln Ser Ser Asp Asp Phe Ala 435 440 445 Leu Ile Val Asn Ala Pro Asn His Glu Gly Ile Gln Ala Gly Val Asn 450 455 460 Arg Phe Tyr Arg Thr Cys Lys Leu Leu Gly Ile Asn Met Ser Lys Lys 465 470 475 480 Lys Ser Tyr Ile Asn Arg Thr Gly Thr Phe Glu Phe Thr Ser Phe Phe 485 490 495 Tyr Arg Tyr Gly Phe Val Ala Asn Phe Gly Met Glu Leu Pro Ser Phe 500 505 510 Gly Val Ser Gly Ile Asn Glu Ser Ala Asp Met Ser Ile Gly Val Thr 515 520 525 Ala Ile Lys Asn Asn Met Ile Asn Asn Asp Leu Gly Pro Ala Thr Ala 530 535 540 Gln Met Ala Leu Gln Leu Phe Ile Lys Asp Tyr Arg Tyr Thr Tyr Arg 545 550 555 560 Cys His Arg Gly Asp Thr Gln Ile Gln Thr Arg Arg Ser Phe Glu Ile 565 570 575 Lys Lys Leu Trp Glu Gln Thr His Ser Lys Ala Gly Leu Leu Val Ser 580 585 590 Asp Gly Gly Pro Asn Leu Tyr Asn Ile Arg Asn Leu His Ile Pro Glu 595 600 605 Val Cys Leu Lys Trp Glu Leu Met Asp Glu Asp Tyr Gln Gly Arg Leu 610 615 620 Cys Asn Pro Leu Asn Pro Phe Val Asn His Lys Asp Ile Glu Ser Val 625 630 635 640 Asn Asn Ala Val Ile Met Pro Ala His Gly Pro Ala Lys Asn Met Gly 645 650 655 Tyr Asp Ala Val Ala Thr Thr His Ser Trp Asp Pro Gln Arg Asn Arg 660 665 670 Ser Ile Leu Asn Thr Ser Gln Arg Gly Ile Leu Glu Asp Glu Gln Met 675 680 685 Tyr Gln Lys Cys Cys Asn Leu Phe Glu Lys Phe Phe Pro Ser Ser Ser 690 695 700 Tyr Arg Arg Pro Val Gly Ile Ser Ser Met Val Glu Ala Met Val Ser 705 710 715 720 Arg Ala Arg Ile Asp Ala Arg Ile Asp Ser Glu Ser Gly Arg Ile Lys 725 730 735 Lys Glu Glu Phe Thr Glu Ile Met Lys Ile Cys Ser Thr Ile Glu Glu 740 745 750 Leu Arg Arg Gln Lys 755 <210> 35 <211> 716 <212> PRT <213> PA of A/swine/Korea/S10/2004(H1N1) <400> 35 Met Glu Asp Phe Val Arg Gln Cys Phe Asn Pro Met Ile Val Glu Leu 1 5 10 15 Ala Glu Lys Ala Met Lys Glu Tyr Gly Glu Asp Leu Lys Val Glu Thr 20 25 30 Asn Lys Phe Ala Ala Ile Cys Thr His Leu Glu Val Cys Phe Met Tyr 35 40 45 Ser Asp Phe His Phe Ile Asp Glu Gln Gly Glu Ser Ile Val Val Glu 50 55 60 Leu Gly Asp Pro Asn Ala Leu Leu Lys His Arg Phe Glu Ile Ile Glu 65 70 75 80 Gly Arg Asp Arg Thr Ile Ala Trp Thr Val Ile Asn Ser Ile Cys Asn 85 90 95 Thr Thr Gly Ala Glu Lys Pro Lys Phe Leu Pro Asp Leu Tyr Asp Tyr 100 105 110 Lys Lys Asn Arg Phe Ile Glu Ile Gly Val Thr Arg Arg Glu Val His 115 120 125 Ile Tyr Tyr Leu Glu Lys Ala Asn Lys Ile Lys Ser Glu Lys Thr His 130 135 140 Ile His Ile Phe Ser Phe Thr Gly Glu Glu Met Ala Thr Lys Ala Asp 145 150 155 160 Tyr Thr Leu Asp Glu Glu Ser Arg Ala Arg Ile Lys Thr Arg Leu Phe 165 170 175 Thr Ile Arg Gln Glu Met Ala Ser Arg Gly Leu Trp Asp Ser Phe Arg 180 185 190 Gln Ser Glu Arg Gly Glu Glu Thr Ile Glu Glu Arg Phe Glu Ile Thr 195 200 205 Gly Thr Met Arg Lys Leu Ala Asp Gln Ser Leu Pro Pro Asn Phe Ser 210 215 220 Ser Leu Glu Lys Ser Arg Ala Tyr Val Asp Gly Phe Glu Pro Asn Gly 225 230 235 240 Tyr Ile Glu Gly Lys Leu Ser Gln Met Ser Lys Glu Val Asn Ala Arg 245 250 255 Ile Glu Pro Phe Leu Lys Ser Thr Pro Arg Pro Leu Arg Leu Pro Asp 260 265 270 Gly Pro Pro Cys Ser Gln Arg Ser Lys Phe Leu Leu Met Asp Ala Leu 275 280 285 Lys Leu Ser Ile Glu Asp Pro Ser His Glu Gly Glu Gly Ile Pro Leu 290 295 300 Tyr Asp Ala Ile Lys Cys Met Arg Thr Phe Phe Gly Trp Lys Glu Pro 305 310 315 320 Asn Val Val Lys Pro His Glu Lys Gly Ile Asn Pro Asn Tyr Leu Leu 325 330 335 Ser Trp Lys Gln Val Leu Ala Glu Leu Gln Asp Ile Glu Asn Glu Glu 340 345 350 Lys Ile Pro Arg Thr Lys Asn Met Lys Lys Thr Ser Gln Leu Lys Trp 355 360 365 Ala Leu Gly Glu Asn Met Ala Pro Glu Lys Val Asp Phe Asp Asp Cys 370 375 380 Lys Asp Val Gly Asp Leu Lys Gln Tyr Asp Ser Asp Glu Pro Glu Leu 385 390 395 400 Arg Ser Leu Ala Ser Trp Ile Gln Asn Glu Phe Asn Lys Ala Cys Glu 405 410 415 Leu Thr Asp Ser Ser Trp Ile Glu Leu Asp Glu Ile Gly Glu Asp Ala 420 425 430 Ala Pro Ile Gly His Ile Ala Ser Met Arg Arg Asn Tyr Phe Thr Ala 435 440 445 Glu Val Ser His Cys Arg Ala Thr Glu Tyr Ile Met Lys Gly Val Tyr 450 455 460 Ile Asn Thr Ala Leu Leu Asn Ala Ser Cys Ala Ala Met Asp Asp Phe 465 470 475 480 Gln Leu Ile Pro Met Ile Ser Lys Cys Arg Thr Lys Glu Gly Arg Arg 485 490 495 Lys Thr Asn Leu Tyr Gly Phe Ile Ile Lys Gly Arg Ser His Leu Arg 500 505 510 Asn Asp Thr Asp Val Val Asn Phe Val Ser Met Glu Phe Ser Leu Thr 515 520 525 Asp Pro Arg Leu Glu Pro His Lys Trp Glu Lys Tyr Cys Val Leu Glu 530 535 540 Val Gly Asp Met Leu Leu Arg Ser Ala Ile Gly His Val Ser Arg Pro 545 550 555 560 Met Phe Leu Tyr Val Arg Thr Asn Gly Thr Ser Lys Ile Lys Met Lys 565 570 575 Trp Gly Met Glu Met Arg Arg Cys Leu Leu Gln Ser Leu Gln Gln Ile 580 585 590 Glu Ser Met Ile Glu Ala Glu Ser Ser Val Lys Glu Lys Asp Met Thr 595 600 605 Lys Glu Phe Phe Glu Asn Lys Ser Glu Thr Trp Pro Val Gly Glu Ser 610 615 620 Pro Lys Gly Val Glu Glu Gly Ser Ile Gly Lys Val Cys Arg Thr Leu 625 630 635 640 Leu Ala Lys Ser Val Phe Asn Ser Leu Tyr Ala Ser Pro Gln Leu Glu 645 650 655 Gly Phe Ser Ala Glu Ser Arg Lys Leu Leu Leu Ile Val Gln Ala Leu 660 665 670 Arg Asp Asn Leu Glu Pro Gly Thr Phe Asp Leu Gly Gly Leu Tyr Glu 675 680 685 Ala Ile Glu Glu Cys Leu Ile Asn Asp Pro Trp Val Leu Leu Asn Ala 690 695 700 Ser Trp Phe Asn Ser Phe Leu Thr His Ala Leu Arg 705 710 715 <210> 36 <211> 289 <212> PRT <213> HA of A/swine/Korea/S10/2004(H1N1) <400> 36 Met Lys Ala Lys Leu Leu Val Leu Leu Tyr Ala Phe Val Ala Thr Asp 1 5 10 15 Ala Asp Thr Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr 20 25 30 Val Asp Thr Ile Leu Glu Lys Asn Val Ala Val Thr His Ser Val Asn 35 40 45 Leu Leu Glu Asp Ser His Asn Gly Lys Leu Cys Lys Leu Lys Gly Ile 50 55 60 Ala Pro Leu Gln Leu Gly Lys Cys Asn Ile Thr Gly Trp Leu Leu Gly 65 70 75 80 Asn Pro Glu Cys Asp Ser Leu Leu Pro Ala Arg Ser Trp Ser Tyr Ile 85 90 95 Val Glu Thr Pro Asn Ser Glu Asn Gly Ala Cys Tyr Pro Gly Asp Leu 100 105 110 Ile Asp Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Leu 115 120 125 Gly Arg Phe Glu Ile Phe Pro Lys Glu Ser Ser Trp Pro Asn His Thr 130 135 140 Phe Asn Gly Val Thr Val Ser Cys Ser His Arg Gly Lys Ser Ser Phe 145 150 155 160 Tyr Arg Asn Leu Leu Trp Leu Thr Lys Lys Gly Asp Ser Tyr Pro Lys 165 170 175 Leu Thr Asn Ser Tyr Val Asn Asn Lys Gly Lys Glu Val Leu Val Leu 180 185 190 Trp Gly Val His His Pro Ser Ser Ser Asp Glu Gln Gln Ser Leu Tyr 195 200 205 Ser Asn Gly Asn Ala Tyr Val Ser Val Ala Ser Ser Asn Tyr Asn Arg 210 215 220 Arg Phe Thr Pro Glu Ile Ala Ala Arg Pro Lys Val Arg Asp Gln His 225 230 235 240 Gly Arg Met Asn Tyr Tyr Trp Thr Leu Leu Glu Pro Gly Asp Thr Ile 245 250 255 Ile Phe Glu Ala Thr Gly Asn Leu Ile Ala Pro Trp Tyr Ala Phe Ala 260 265 270 Leu Ser Arg Gly Phe Glu Ser Gly Ile Ile Thr Ser Asn Ala Ser Met 275 280 285 His <210> 37 <211> 180 <212> PRT <213> NP of A/swine/Korea/S10/2004(H1N1) <400> 37 Glu Thr Asp Gly Glu Arg Gln Asn Ala Thr Glu Ile Arg Ala Ser Val 1 5 10 15 Gly Lys Met Ile Asp Gly Ile Gly Arg Phe Tyr Ile Gln Met Cys Thr 20 25 30 Glu Leu Lys Leu Ser Asp Tyr Glu Gly Arg Leu Ile Gln Asn Ser Leu 35 40 45 Thr Ile Glu Arg Met Val Leu Ser Ala Phe Asp Glu Arg Arg Asn Lys 50 55 60 Tyr Leu Glu Glu His Pro Ser Ala Gly Lys Asp Pro Lys Lys Thr Gly 65 70 75 80 Gly Pro Ile Tyr Arg Arg Val Asp Gly Lys Trp Arg Arg Glu Leu Ile 85 90 95 Leu Tyr Asp Lys Glu Glu Ile Arg Arg Ile Trp Arg Gln Ala Asn Asn 100 105 110 Gly Asp Asp Ala Thr Ala Gly Leu Thr His Met Met Ile Trp His Ser 115 120 125 Asn Leu Asn Asp Ala Thr Tyr Gln Arg Thr Arg Thr Leu Val Arg Thr 130 135 140 Gly Met Asp Pro Arg Met Cys Ser Leu Met Gln Gly Ser Thr Leu Pro 145 150 155 160 Arg Arg Ser Gly Ala Ala Gly Ala Ala Val Lys Gly Val Gly Thr Met 165 170 175 Val Arg Glu Leu 180 <210> 38 <211> 365 <212> PRT <213> NA of A/swine/Korea/S10/2004(H1N1) <400> 38 Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Ile Cys Met Val 1 5 10 15 Val Gly Ile Ile Ser Leu Ile Leu Gln Ile Gly Asn Ile Ile Ser Ile 20 25 30 Trp Ile Ser His Ser Ile Gln Thr Gly Asn Gln Asn His Thr Gly Ile 35 40 45 Cys Asn Gln Gly Ser Ile Thr Tyr Lys Val Val Ala Gly Gln Asp Ser 50 55 60 Thr Ser Val Ile Leu Thr Gly Asn Ser Ser Leu Cys Pro Ile Arg Gly 65 70 75 80 Trp Ala Ile His Ser Lys Asp Asn Gly Ile Arg Ile Gly Ser Lys Gly 85 90 95 Asp Val Phe Val Ile Arg Glu Pro Phe Ile Ser Cys Ser His Leu Glu 100 105 110 Cys Arg Thr Phe Phe Leu Thr Gln Gly Ala Leu Leu Asn Asp Lys His 115 120 125 Ser Arg Gly Thr Phe Lys Asp Arg Ser Pro Tyr Arg Ala Leu Met Ser 130 135 140 Cys Pro Val Gly Glu Ala Pro Ser Pro Tyr Asn Ser Arg Phe Glu Ser 145 150 155 160 Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Met Gly Trp Leu Thr 165 170 175 Ile Gly Ile Ser Gly Pro Asp Asp Gly Ala Val Ala Val Leu Lys Tyr 180 185 190 Asn Arg Ile Ile Thr Glu Thr Ile Lys Ser Trp Arg Lys Asn Ile Leu 195 200 205 Arg Thr Gln Glu Ser Glu Cys Thr Cys Val Asn Gly Ser Cys Phe Thr 210 215 220 Ile Met Thr Asp Gly Pro Ser Asp Gly Leu Ala Ser Tyr Lys Ile Phe 225 230 235 240 Lys Ile Glu Lys Gly Lys Val Thr Lys Ser Ile Glu Leu Asn Ala Pro 245 250 255 Asn Ser His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Thr Gly Lys Val 260 265 270 Met Cys Val Cys Arg Asp Asn Trp His Gly Ser Asn Arg Pro Trp Val 275 280 285 Ser Phe Asp Gln Asn Leu Asp Tyr Lys Ile Gly Tyr Ile Cys Ser Gly 290 295 300 Val Phe Gly Asp Asn Pro Arg Pro Lys Asp Gly Thr Gly Ser Cys Gly 305 310 315 320 Pro Val Ser Ala Asp Gly Val Asn Gly Val Lys Gly Phe Ser Tyr Lys 325 330 335 Tyr Gly Asn Gly Val Trp Ile Gly Arg Thr Lys Ser Asp Ser Ser Arg 340 345 350 His Gly Phe Glu Met Ile Trp Asp Pro Asn Gly Trp Thr 355 360 365 <210> 39 <211> 252 <212> PRT <213> M of A/swine/Korea/S10/2004(H1N1) <400> 39 Met Ser Leu Leu Thr Glu Val Glu Thr Tyr Val Leu Ser Ile Val Pro 1 5 10 15 Ser Gly Pro Leu Lys Ala Glu Ile Ala Gln Arg Leu Glu Asp Val Phe 20 25 30 Ala Gly Lys Asn Thr Asp Leu Glu Val Leu Met Glu Trp Leu Lys Thr 35 40 45 Arg Pro Ile Leu Ser Pro Leu Thr Lys Gly Ile Leu Gly Phe Val Phe 50 55 60 Thr Leu Thr Val Pro Ser Glu Arg Gly Leu Gln Arg Arg Arg Phe Val 65 70 75 80 Gln Asn Ala Leu Asn Gly Asn Gly Asp Pro Asn Asn Met Asp Lys Ala 85 90 95 Val Lys Leu Tyr Arg Lys Leu Lys Arg Glu Ile Thr Phe His Gly Ala 100 105 110 Lys Glu Ile Ala Leu Ser Tyr Ser Ala Gly Ala Leu Ala Cys Cys Met 115 120 125 Gly Leu Ile Tyr Asn Arg Met Gly Ala Val Thr Thr Glu Val Ala Phe 130 135 140 Gly Leu Val Cys Ala Thr Cys Glu Gln Ile Ala Asp Ser Gln His Arg 145 150 155 160 Ser His Arg Gln Met Val Thr Thr Thr Asn Pro Leu Ile Arg His Glu 165 170 175 Asn Arg Met Val Leu Ala Ser Thr Thr Ala Lys Ala Met Glu Gln Met 180 185 190 Ala Gly Ser Ser Glu Gln Ala Ala Glu Ala Met Asp Ile Ala Ser Gln 195 200 205 Ala Arg Gln Met Val Gln Ala Met Arg Thr Val Gly Thr His Pro Ser 210 215 220 Ser Ser Ala Gly Leu Lys Asp Asp Leu Leu Glu Asn Leu Gln Ala Tyr 225 230 235 240 Gln Lys Arg Met Gly Val Gln Met Gln Arg Phe Lys 245 250 <210> 40 <211> 230 <212> PRT <213> NS of A/swine/Korea/S10/2004(H1N1) <400> 40 Met Asp Pro Asn Thr Val Ser Ser Phe Gln Val Asp Cys Phe Leu Trp 1 5 10 15 His Val Arg Lys Arg Val Ala Asp Gln Glu Leu Gly Asp Ala Pro Phe 20 25 30 Leu Asp Arg Leu Arg Arg Asp Gln Lys Ser Leu Arg Gly Arg Gly Ser 35 40 45 Thr Leu Gly Leu Asp Ile Glu Thr Ala Thr Arg Ala Gly Lys Gln Ile 50 55 60 Val Glu Arg Ile Leu Lys Glu Glu Ser Asp Glu Ala Leu Glu Met Thr 65 70 75 80 Met Ala Ser Val Pro Ala Ser Arg Tyr Leu Thr Asp Met Thr Leu Glu 85 90 95 Glu Met Ser Arg His Trp Phe Met Leu Met Pro Lys Gln Lys Val Ala 100 105 110 Gly Pro Leu Cys Ile Arg Met Asp Gln Ala Ile Met Asp Lys Asn Ile 115 120 125 Ile Leu Lys Ala Asn Phe Ser Val Ile Phe Asp Arg Leu Glu Thr Leu 130 135 140 Ile Leu Leu Arg Ala Phe Thr Glu Glu Gly Thr Ile Val Gly Glu Ile 145 150 155 160 Ser Pro Leu Pro Ser Leu Pro Gly His Thr Asp Glu Asp Val Lys Asn 165 170 175 Ala Val Gly Val Leu Ile Gly Gly Leu Glu Trp Asn Asn Asn Thr Val 180 185 190 Arg Val Ser Glu Thr Leu Gln Arg Phe Ala Trp Arg Ser Ser Asn Glu 195 200 205 Asn Gly Arg Pro Pro Leu Thr Pro Lys Gln Lys Arg Lys Met Ala Gly 210 215 220 Thr Ile Thr Ser Glu Val 225 230 <210> 41 <211> 369 <212> PRT <213> PB2 of A/swine/Korea/S109/2004(H9N2) <400> 41 Glu Gln Ser Ile Ala Glu Ala Ile Ile Val Ala Met Val Phe Ser Gln 1 5 10 15 Glu Asp Cys Met Ile Lys Ala Val Arg Gly Asp Leu Asn Phe Met Asn 20 25 30 Arg Ala Asn Gln Arg Leu Asn Pro Met His Gln Leu Leu Arg His Phe 35 40 45 Gln Lys Asp Ala Lys Val Leu Phe Gln Asn Trp Gly Ile Glu Pro Ile 50 55 60 Asp Asn Val Met Gly Met Ile Gly Ile Leu Pro Asp Met Thr Pro Ser 65 70 75 80 Thr Glu Met Ser Leu Arg Gly Val Arg Val Ser Lys Met Gly Val Asp 85 90 95 Glu Tyr Ser Ser Thr Glu Arg Val Val Val Ser Ile Asp Arg Phe Leu 100 105 110 Arg Val Arg Asp Gln Arg Gly Asn Ile Leu Leu Ser Pro Glu Glu Val 115 120 125 Ser Glu Thr Gln Gly Thr Glu Lys Leu Thr Ile Thr Tyr Ser Ser Ser 130 135 140 Met Met Trp Glu Ile Asn Gly Pro Glu Ser Val Leu Val Asn Thr Tyr 145 150 155 160 Gln Trp Ile Ile Arg Asn Trp Glu Thr Val Lys Ile Gln Trp Ser Gln 165 170 175 Asp Pro Thr Met Leu Tyr Asn Lys Val Glu Phe Glu Pro Phe Gln Ser 180 185 190 Leu Val Pro Lys Ala Ala Arg Gly Gln Tyr Ser Gly Phe Val Arg Thr 195 200 205 Leu Phe Gln Gln Met Arg Asp Val Pro Gly Thr Phe Asp Thr Val Gln 210 215 220 Ile Ile Lys Leu Leu Pro Phe Ala Ala Ala Pro Pro Glu Gln Ser Arg 225 230 235 240 Met Gln Phe Ser Ser Leu Thr Val Asn Val Arg Gly Ser Gly Met Arg 245 250 255 Ile Leu Val Arg Gly Asn Ser Pro Val Phe Asn Tyr Asn Lys Ala Thr 260 265 270 Lys Arg Leu Thr Val Leu Gly Lys Asp Ala Gly Ala Leu Thr Glu Asp 275 280 285 Pro Asp Glu Gly Thr Ala Gly Val Glu Ser Ala Val Leu Arg Gly Phe 290 295 300 Leu Ile Leu Gly Lys Glu Asp Lys Arg Tyr Gly Pro Ala Leu Ser Ile 305 310 315 320 Asn Glu Leu Ser Asn Leu Ala Lys Gly Glu Lys Ala Asn Val Leu Ile 325 330 335 Gly Gln Gly Asp Val Val Leu Val Met Lys Arg Lys Arg Asp Ser Ser 340 345 350 Ile Leu Thr Asp Ser Gln Thr Ala Thr Lys Arg Ile Arg Met Ala Ile 355 360 365 Asn <210> 42 <211> 757 <212> PRT <213> PB1 of A/swine/Korea/S109/2004(H9N2) <400> 42 Met Asp Val Asn Pro Thr Leu Leu Phe Leu Lys Val Pro Ala Gln Asn 1 5 10 15 Ala Ile Ser Thr Thr Phe Pro Tyr Thr Gly Asp Pro Pro Tyr Ser His 20 25 30 Gly Thr Gly Thr Gly Tyr Thr Met Asp Thr Val Asn Arg Thr His Gln 35 40 45 Tyr Ser Glu Arg Gly Arg Trp Thr Thr Asn Thr Glu Thr Gly Ala Pro 50 55 60 Gln Leu Asn Pro Ile Asp Gly Pro Leu Pro Glu Asp Asn Glu Pro Ser 65 70 75 80 Gly Tyr Ala Gln Thr Asp Cys Val Leu Glu Ala Met Ala Phe Leu Glu 85 90 95 Glu Ser His Pro Gly Ile Phe Glu Thr Ser Cys Leu Glu Thr Met Glu 100 105 110 Val Val Gln Gln Thr Arg Val Asp Lys Leu Thr Gln Gly Arg Gln Thr 115 120 125 Tyr Asp Trp Thr Leu Asn Arg Asn Gln Pro Ala Ala Thr Ala Leu Ala 130 135 140 Asn Thr Ile Glu Val Phe Arg Ser Asn Gly Leu Thr Ala Asn Glu Ser 145 150 155 160 Gly Arg Leu Ile Asp Phe Leu Lys Asp Val Met Glu Ser Met Asn Lys 165 170 175 Glu Glu Met Glu Ile Thr Thr His Phe Gln Arg Lys Arg Arg Val Arg 180 185 190 Asp Asn Met Thr Lys Lys Met Val Thr Gln Arg Thr Ile Gly Lys Arg 195 200 205 Lys Gln Arg Leu Asn Lys Arg Ser Tyr Leu Ile Arg Ala Leu Thr Leu 210 215 220 Asn Thr Met Thr Lys Asp Ala Glu Arg Gly Lys Leu Lys Arg Arg Ala 225 230 235 240 Ile Ala Thr Pro Gly Met Gln Ile Arg Gly Phe Val Tyr Phe Val Glu 245 250 255 Thr Leu Ala Arg Ser Ile Cys Glu Lys Leu Glu Gln Ser Gly Leu Pro 260 265 270 Val Gly Gly Asn Glu Lys Lys Ala Lys Leu Ala Asn Val Val Arg Lys 275 280 285 Met Met Thr Asn Ser Gln Asp Thr Glu Ile Ser Phe Thr Ile Thr Gly 290 295 300 Asp Asn Thr Lys Trp Asn Glu Asn Gln Asn Pro Arg Met Phe Leu Ala 305 310 315 320 Met Ile Thr Tyr Ile Thr Arg Asn Gln Pro Glu Trp Phe Arg Asn Val 325 330 335 Leu Ser Ile Ala Pro Ile Met Phe Ser Asn Lys Met Ala Arg Leu Gly 340 345 350 Lys Gly Tyr Met Phe Glu Ser Lys Ser Met Lys Ile Arg Thr Gln Ile 355 360 365 Pro Ala Glu Met Leu Ala Ser Ile Asp Leu Lys Tyr Phe Asn Asp Ser 370 375 380 Thr Arg Lys Lys Ile Glu Lys Ile Arg Pro Leu Leu Ile Asp Gly Thr 385 390 395 400 Ala Ser Leu Ser Pro Gly Met Met Met Gly Met Phe Asn Met Leu Ser 405 410 415 Thr Val Leu Gly Val Ser Ile Leu Asn Leu Gly Gln Lys Arg His Thr 420 425 430 Lys Thr Thr Tyr Trp Trp Asp Gly Leu Gln Ser Ser Asp Asp Phe Ala 435 440 445 Leu Ile Val Asn Ala Pro Asn His Glu Gly Ile Gln Ala Gly Val Asn 450 455 460 Arg Phe Tyr Arg Thr Cys Lys Leu Leu Gly Ile Asn Met Ser Lys Lys 465 470 475 480 Lys Ser Tyr Ile Asn Arg Thr Gly Thr Phe Glu Phe Thr Ser Phe Phe 485 490 495 Tyr Arg Tyr Gly Phe Val Ala Lys Phe Ser Val Glu Leu Pro Ser Phe 500 505 510 Gly Val Ser Gly Ile Asn Glu Ser Ala Asp Met Ser Ile Gly Val Thr 515 520 525 Val Ile Lys Asn Asn Met Ile Asn Asn Asp Leu Gly Pro Ala Thr Ala 530 535 540 Gln Met Ala Leu Gln Leu Phe Ile Lys Asp Tyr Arg Tyr Thr Tyr Arg 545 550 555 560 Cys His Arg Gly Asp Thr Gln Ile Gln Thr Arg Arg Ser Phe Glu Ile 565 570 575 Lys Lys Pro Trp Glu Gln Thr His Ser Lys Ala Gly Leu Leu Val Ser 580 585 590 Asp Gly Gly Pro Asn Leu Tyr Asn Ile Arg Asn Leu His Ile Pro Glu 595 600 605 Val Cys Leu Lys Trp Glu Leu Met Asp Glu Asp Tyr Gln Gly Arg Leu 610 615 620 Cys Asn Pro Leu Asn Pro Phe Val Asn His Lys Asp Ile Glu Ser Val 625 630 635 640 Asn Asn Ala Val Ile Met Pro Ala His Gly Pro Ala Lys Asn Met Glu 645 650 655 Tyr Asp Ala Val Ala Thr Thr His Ser Trp Ile Pro Lys Arg Asn Arg 660 665 670 Ser Ile Leu Asn Thr Ser Gln Arg Gly Ile Leu Glu Asp Glu Gln Met 675 680 685 Tyr Gln Lys Cys Cys Asn Leu Phe Glu Lys Phe Phe Pro Ser Ser Ser 690 695 700 Tyr Arg Arg Pro Val Gly Ile Ser Ser Met Val Glu Ala Met Val Ser 705 710 715 720 Arg Ala Arg Ile Asp Ala Arg Ile Asp Phe Glu Ser Gly Arg Ile Lys 725 730 735 Lys Glu Glu Phe Thr Glu Ile Met Lys Ile Cys Ser Thr Ile Glu Glu 740 745 750 Leu Arg Arg Gln Lys 755 <210> 43 <211> 716 <212> PRT <213> PA of A/swine/Korea/S109/2004(H9N2) <400> 43 Met Glu Asp Phe Val Arg Gln Cys Phe Asn Pro Met Ile Val Glu Leu 1 5 10 15 Ala Glu Lys Ala Met Lys Glu Tyr Gly Glu Asp Leu Lys Ile Glu Thr 20 25 30 Asn Lys Phe Ala Ala Ile Cys Thr His Leu Glu Val Cys Phe Met Tyr 35 40 45 Ser Asp Phe His Phe Ile Asp Glu Gln Gly Glu Ser Ile Val Val Glu 50 55 60 Leu Gly Asp Pro Asn Ala Leu Leu Lys His Arg Phe Glu Ile Ile Glu 65 70 75 80 Gly Arg Asp Arg Thr Ile Ala Trp Thr Val Ile Asn Ser Ile Cys Asn 85 90 95 Thr Thr Gly Ala Glu Lys Pro Lys Phe Leu Pro Asp Leu Tyr Asp Tyr 100 105 110 Lys Lys Asn Arg Phe Ile Glu Ile Gly Val Thr Arg Arg Glu Val His 115 120 125 Ile Tyr Tyr Leu Glu Lys Ala Asn Lys Ile Lys Ser Glu Lys Thr His 130 135 140 Ile His Ile Phe Ser Phe Thr Gly Glu Glu Met Ala Thr Lys Ala Asp 145 150 155 160 Tyr Thr Leu Asp Glu Glu Ser Arg Ala Arg Ile Lys Thr Arg Leu Phe 165 170 175 Thr Ile Arg Gln Glu Met Ala Ser Arg Gly Leu Trp Asp Ser Phe Arg 180 185 190 Gln Ser Glu Arg Gly Glu Glu Thr Ile Glu Glu Arg Phe Glu Ile Thr 195 200 205 Gly Thr Met Arg Lys Leu Ala Asp Gln Ser Leu Pro Pro Asn Phe Ser 210 215 220 Ser Leu Glu Lys Phe Arg Ala Tyr Val Asp Gly Phe Glu Pro Asn Gly 225 230 235 240 Tyr Ile Glu Gly Lys Leu Ser Gln Met Ser Lys Glu Val Asn Ala Arg 245 250 255 Ile Glu Pro Phe Leu Lys Ser Thr Pro Arg Pro Leu Arg Leu Pro Asp 260 265 270 Gly Pro Pro Cys Ser Gln Arg Ser Lys Ser Leu Leu Met Asp Ala Leu 275 280 285 Lys Leu Ser Ile Glu Asp Pro Ser His Glu Gly Glu Gly Ile Pro Leu 290 295 300 Tyr Asp Ala Ile Lys Cys Met Arg Thr Phe Phe Gly Trp Lys Glu Pro 305 310 315 320 Asn Val Val Lys Pro His Glu Lys Gly Ile Asn Pro Asn Tyr Leu Leu 325 330 335 Ser Trp Lys Gln Val Leu Ala Glu Leu Gln Asp Ile Glu Asn Glu Glu 340 345 350 Lys Ile Pro Arg Thr Lys Asn Met Lys Lys Thr Ser Gln Leu Lys Trp 355 360 365 Ala Leu Gly Glu Asn Met Ala Pro Glu Lys Val Asp Phe Asp Asp Cys 370 375 380 Lys Asp Val Gly Asp Leu Lys Gln Tyr Asp Ser Asp Glu Pro Glu Leu 385 390 395 400 Arg Ser Leu Ala Ser Trp Ile Gln Asn Glu Phe Asn Lys Ala Cys Glu 405 410 415 Leu Thr Asp Ser Ser Trp Ile Glu Leu Asp Glu Ile Gly Glu Asp Ala 420 425 430 Ala Pro Ile Glu His Ile Ala Ser Met Arg Arg Asn Tyr Phe Thr Ala 435 440 445 Glu Val Ser His Cys Arg Ala Thr Glu Tyr Ile Met Lys Gly Val Tyr 450 455 460 Ile Asn Thr Ala Leu Leu Asn Ala Ser Cys Ala Ala Met Asp Asp Phe 465 470 475 480 Gln Leu Ile Pro Met Ile Ser Lys Cys Arg Thr Lys Glu Gly Arg Arg 485 490 495 Lys Thr Asn Leu Tyr Gly Phe Ile Ile Lys Gly Arg Ser His Leu Arg 500 505 510 Asn Asp Thr Asp Val Val Asn Phe Val Ser Met Glu Phe Ser Leu Thr 515 520 525 Asp Pro Arg Leu Glu Pro His Lys Trp Glu Lys Tyr Cys Val Leu Glu 530 535 540 Val Gly Asp Met Leu Leu Arg Ser Ala Ile Gly His Val Ser Arg Pro 545 550 555 560 Met Phe Leu Tyr Val Arg Thr Asn Gly Thr Ser Lys Ile Lys Met Lys 565 570 575 Trp Gly Met Glu Met Arg Arg Cys Leu Leu Gln Ser Leu Gln Gln Ile 580 585 590 Glu Ser Met Ile Glu Ala Glu Ser Ser Val Lys Glu Lys Asp Met Thr 595 600 605 Lys Glu Phe Phe Glu Asn Lys Ser Glu Thr Trp Pro Val Gly Glu Ser 610 615 620 Pro Lys Gly Val Glu Glu Gly Ser Ile Gly Lys Val Cys Arg Thr Leu 625 630 635 640 Leu Ala Lys Ser Val Phe Asn Ser Leu Tyr Ala Ser Pro Gln Leu Glu 645 650 655 Gly Phe Ser Ala Glu Ser Arg Lys Leu Leu Leu Ile Val Gln Ala Leu 660 665 670 Arg Asp Asn Leu Glu Pro Gly Thr Phe Asp Leu Gly Gly Leu Tyr Glu 675 680 685 Ala Ile Glu Glu Cys Leu Ile Asn Asp Pro Trp Val Leu Leu Asn Ala 690 695 700 Ser Trp Phe Asn Ser Phe Leu Thr His Ala Leu Arg 705 710 715 <210> 44 <211> 368 <212> PRT <213> HA of A/swine/Korea/S109/2004(H9N2) <400> 44 Met Glu Val Ile Ala Leu Ile Ala Ile Leu Val Val Thr Gly Thr Ser 1 5 10 15 Asn Ala Asp Lys Ile Cys Ile Gly Tyr Gln Ser Thr Asn Ser Thr Glu 20 25 30 Thr Val Asp Thr Leu Val Glu Thr Asn Val Pro Val Thr His Ala Lys 35 40 45 Glu Leu Leu His Thr Glu His Asn Gly Met Leu Cys Ala Thr Asn Gln 50 55 60 Gly Arg Pro Leu Ile Leu Asp Thr Cys Thr Val Glu Gly Leu Val Tyr 65 70 75 80 Gly Asn Pro Ser Cys Asp Leu Leu Leu Gly Gly Lys Glu Trp Ser Tyr 85 90 95 Ile Val Lys Arg Ser Ser Ala Ile Asn Gly Met Cys Tyr Pro Gly Arg 100 105 110 Val Glu Asn Leu Glu Glu Leu Arg Ser Phe Phe Ser Ser Ala Arg Ser 115 120 125 Tyr Lys Arg Leu Leu Leu Phe Pro Asp Arg Thr Trp Asn Val Thr Tyr 130 135 140 Thr Gly Thr Ser Lys Ala Cys Ser Gly Ser Phe Tyr Arg Ser Met Arg 145 150 155 160 Trp Leu Thr His Lys Ser Asn Ser Tyr Pro Ile Gln Asp Ala Gln Tyr 165 170 175 Thr Asn Asp Trp Gly Lys Asn Ile Leu Phe Met Trp Gly Ile His His 180 185 190 Pro Pro Thr Asp Thr Glu Gln Met Asn Leu Tyr Lys Lys Ala Asp Thr 195 200 205 Thr Thr Ser Ile Thr Thr Glu Asp Ile Asn Arg Thr Phe Lys Pro Val 210 215 220 Ile Gly Pro Arg Pro Leu Val Asn Gly Gln Gln Gly Arg Ile Asp Tyr 225 230 235 240 Tyr Trp Ser Val Leu Lys Pro Gly Gln Thr Leu Arg Ile Arg Ser Asn 245 250 255 Gly Asn Leu Ile Ala Pro Trp Tyr Gly His Ile Pro Ser Gly Glu Ser 260 265 270 His Gly Arg Ile Leu Lys Thr Asp Leu Asn Ser Gly Asn Cys Val Val 275 280 285 Gln Cys Gln Thr Glu Lys Gly Gly Leu Asn Thr Thr Leu Pro Phe His 290 295 300 Asn Val Ser Lys Tyr Ala Phe Gly Asn Cys Pro Lys Tyr Val Gly Val 305 310 315 320 Lys Ser Leu Lys Leu Ala Val Gly Leu Arg Asn Val Pro Ala Ala Ser 325 330 335 Gly Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp 340 345 350 Pro Gly Leu Val Ala Gly Trp Tyr Gly Phe Gln His Ser Asn Asp Gln 355 360 365 <210> 45 <211> 180 <212> PRT <213> NP of A/swine/Korea/S109/2004(H9N2) <400> 45 Glu Thr Asp Gly Glu Arg Gln Asn Ala Thr Glu Ile Arg Ala Ser Val 1 5 10 15 Gly Lys Met Ile Asp Gly Ile Gly Arg Phe Tyr Ile Gln Met Cys Thr 20 25 30 Glu Leu Lys Leu Ser Asp Tyr Glu Gly Arg Leu Ile Gln Asn Ser Leu 35 40 45 Thr Ile Glu Arg Met Val Leu Ser Ala Phe Asp Glu Arg Arg Asn Lys 50 55 60 Tyr Leu Glu Glu His Pro Ser Ala Gly Lys Asp Pro Lys Lys Thr Gly 65 70 75 80 Gly Pro Ile Tyr Arg Arg Val Asp Gly Lys Trp Arg Arg Glu Leu Ile 85 90 95 Leu Tyr Asp Lys Glu Glu Ile Arg Arg Ile Trp Arg Gln Ala Asn Asn 100 105 110 Gly Asp Asp Ala Thr Ala Gly Leu Thr His Met Met Ile Trp His Ser 115 120 125 Asn Leu Asn Asp Ala Thr Tyr Gln Arg Thr Arg Ala Leu Val Arg Thr 130 135 140 Gly Met Asp Pro Arg Met Cys Ser Leu Met Gln Gly Ser Thr Leu Pro 145 150 155 160 Arg Arg Ser Gly Ala Ala Gly Ala Ala Val Lys Gly Val Gly Thr Met 165 170 175 Val Met Glu Leu 180 <210> 46 <211> 469 <212> PRT <213> NA of A/swine/Korea/S109/2004(H9N2) <400> 46 Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Ser Leu Thr 1 5 10 15 Ile Ala Thr Val Cys Phe Leu Met Gln Ile Ala Ile Leu Ala Thr Thr 20 25 30 Val Thr Leu His Phe Lys Gln Asn Glu Gly Ser Ile Pro Ser Asn Asn 35 40 45 Gln Val Val Pro Cys Glu Pro Ile Ile Ile Glu Arg Asn Ile Thr Glu 50 55 60 Ile Val Tyr Leu Asn Asn Thr Ile Ile Glu Lys Glu Leu Cys Pro Lys 65 70 75 80 Val Val Lys Tyr Arg Ser Trp Ser Lys Pro Gln Cys Gln Ile Thr Gly 85 90 95 Phe Ala Pro Phe Ser Lys Asp Asp Ser Ile Arg Leu Ser Ala Gly Gly 100 105 110 Ser Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Ser Ser Asn Lys 115 120 125 Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asp Asn Arg His 130 135 140 Ser Asn Gly Thr Ile His Asp Arg Ile Pro His Arg Thr Leu Leu Met 145 150 155 160 Asn Glu Leu Gly Val Pro Phe His Leu Gly Thr Lys Gln Val Cys Ile 165 170 175 Ala Trp Ser Ser Ser Ser Cys His Asp Gly Lys Ala Trp Leu His Val 180 185 190 Cys Val Thr Gly Asp Asp Arg Asn Ala Thr Ala Ser Phe Ile Tyr Asp 195 200 205 Gly Thr Leu Val Asp Thr Ile Gly Ser Trp Ser Gln Asn Ile Leu Arg 210 215 220 Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Thr Cys Thr Val Val 225 230 235 240 Met Thr Asp Gly Ser Ala Ser Gly Arg Ala Asp Thr Arg Val Leu Phe 245 250 255 Ile Lys Glu Gly Lys Ile Val His Ile Ser Gln Leu Ser Gly Ser Ala 260 265 270 Gln His Ile Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Asp Val Arg 275 280 285 Cys Val Tyr Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Ile Ile Asp 290 295 300 Ile Lys Met Ala Asp Tyr Ser Ile Asp Ser Ser Tyr Val Cys Ser Gly 305 310 315 320 Leu Val Gly Asp Thr Pro Arg Asp Asp Asp Ser Ser Ser Asn Ser Asn 325 330 335 Cys Lys Asp Pro Asn Asn Glu Arg Gly Asn Pro Gly Val Lys Gly Trp 340 345 350 Ala Phe Asp Tyr Gly Asn Asp Val Trp Met Gly Arg Thr Ile Ser Lys 355 360 365 Asp Ser Arg Ser Gly Tyr Glu Thr Phe Lys Val Ile Asp Gly Trp Thr 370 375 380 Thr Ala Asn Ser Lys Ser Gln Val Asn Arg Gln Val Ile Val Asp Asn 385 390 395 400 Asn Asn Trp Ser Gly Tyr Pro Gly Ile Phe Ser Val Glu Gly Lys Ser 405 410 415 Cys Val Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Pro Gln 420 425 430 Glu Thr Arg Val Trp Trp Thr Ser Asn Ser Ile Val Val Phe Cys Gly 435 440 445 Thr Ser Gly Thr Tyr Gly Thr Gly Ser Trp Pro Asp Gly Ala Asn Ile 450 455 460 Asn Phe Met Pro Ile 465 <210> 47 <211> 252 <212> PRT <213> M of A/swine/Korea/S109/2004(H9N2) <400> 47 Met Ser Leu Leu Thr Glu Val Glu Thr Tyr Val Leu Ser Ile Val Pro 1 5 10 15 Ser Gly Pro Leu Lys Ala Glu Ile Ala Gln Arg Leu Glu Asp Val Phe 20 25 30 Ala Gly Lys Asn Thr Asp Leu Glu Val Leu Met Glu Trp Leu Lys Thr 35 40 45 Arg Pro Ile Leu Ser Pro Leu Thr Lys Gly Ile Leu Gly Phe Val Phe 50 55 60 Thr Leu Thr Val Pro Ser Glu Arg Gly Leu Gln Arg Arg Arg Phe Val 65 70 75 80 Gln Asn Ala Leu Asn Gly Asn Gly Asp Pro Asn Asn Met Asp Lys Ala 85 90 95 Val Lys Leu Tyr Arg Lys Leu Lys Arg Glu Ile Thr Phe His Gly Ala 100 105 110 Lys Glu Ile Ala Leu Ser Tyr Ser Ala Gly Ala Leu Ala Cys Cys Met 115 120 125 Gly Leu Ile Tyr Asn Arg Met Gly Ala Val Thr Thr Glu Ala Ala Phe 130 135 140 Gly Leu Val Cys Ala Thr Cys Glu Gln Ile Ala Asp Ser Gln His Arg 145 150 155 160 Ser His Arg Gln Met Val Thr Thr Thr Asn Pro Leu Ile Arg His Glu 165 170 175 Asn Arg Met Val Leu Ala Ser Thr Thr Ala Lys Ala Met Glu Gln Met 180 185 190 Ala Gly Ser Ser Glu Gln Ala Ala Glu Ala Met Asp Ile Ala Ser Gln 195 200 205 Ala Arg Gln Met Val Gln Ala Thr Arg Thr Val Gly Thr His Pro Ser 210 215 220 Ser Ser Ala Gly Leu Lys Asp Asp Leu Leu Glu Asn Leu Gln Ala Tyr 225 230 235 240 Gln Lys Arg Met Gly Val Gln Met Gln Arg Phe Lys 245 250 <210> 48 <211> 230 <212> PRT <213> NS of A/swine/Korea/S109/2004(H9N2) <400> 48 Met Asp Pro Asn Thr Val Ser Ser Phe Gln Val Asp Cys Phe Leu Trp 1 5 10 15 His Val Arg Lys Arg Val Ala Asp Gln Glu Leu Gly Asp Ala Pro Phe 20 25 30 Leu Asp Arg Leu Arg Arg Asp Gln Lys Ser Leu Arg Gly Arg Gly Ser 35 40 45 Thr Leu Gly Leu Asp Ile Glu Thr Ala Thr Arg Ala Gly Lys Gln Ile 50 55 60 Val Glu Arg Ile Leu Lys Glu Glu Ser Asp Glu Ala Leu Lys Met Thr 65 70 75 80 Met Ala Ser Val Pro Ala Ser Arg Tyr Leu Thr Asp Met Thr Leu Glu 85 90 95 Glu Met Ser Arg His Trp Phe Met Leu Met Pro Lys Gln Lys Val Ala 100 105 110 Gly Pro Leu Cys Ile Arg Met Asp Gln Ala Ile Met Asp Lys Asn Ile 115 120 125 Ile Leu Lys Ala Asn Phe Ser Val Ile Phe Asp Arg Leu Glu Thr Leu 130 135 140 Ile Leu Leu Arg Ala Phe Ala Glu Glu Gly Thr Ile Val Gly Glu Ile 145 150 155 160 Ser Pro Leu Pro Ser Leu Pro Gly His Thr Asp Glu Asp Val Lys Asn 165 170 175 Ala Val Gly Val Leu Ile Gly Gly Leu Glu Trp Asn Asn Asn Thr Val 180 185 190 Arg Val Ser Glu Thr Leu Gln Arg Phe Ala Trp Arg Ser Ser Asn Glu 195 200 205 Asn Gly Arg Pro Pro Leu Thr Pro Lys Gln Lys Arg Lys Met Ala Gly 210 215 220 Thr Ile Arg Ser Glu Val 225 230 <210> 49 <211> 366 <212> PRT <213> PB2 of A/swine/Korea/S81/2004(H9N2) <400> 49 Ser Ile Ala Glu Ala Ile Ile Val Ala Met Val Phe Ser Gln Glu Asp 1 5 10 15 Cys Met Ile Lys Ala Val Arg Gly Asp Leu Asn Phe Met Asn Arg Ala 20 25 30 Asn Gln Arg Leu Asn Pro Met His Gln Leu Leu Arg His Phe Gln Lys 35 40 45 Asp Ala Lys Val Leu Phe Gln Asn Trp Gly Ile Glu Pro Ile Asp Asn 50 55 60 Val Met Gly Met Ile Gly Ile Leu Pro Asp Met Thr Pro Ser Thr Glu 65 70 75 80 Met Ser Leu Arg Gly Val Arg Val Ser Lys Met Gly Val Asp Glu Tyr 85 90 95 Ser Ser Thr Glu Arg Val Val Val Ser Ile Asp Arg Phe Leu Arg Val 100 105 110 Arg Asp Gln Arg Gly Asn Ile Leu Leu Ser Pro Glu Glu Val Ser Glu 115 120 125 Thr Gln Gly Thr Glu Lys Leu Thr Ile Thr Tyr Ser Ser Ser Met Met 130 135 140 Trp Glu Ile Asn Gly Pro Glu Ser Val Leu Val Asn Thr Tyr Gln Trp 145 150 155 160 Ile Ile Arg Asn Trp Glu Thr Val Lys Ile Gln Trp Ser Gln Asp Pro 165 170 175 Thr Met Leu Tyr Asn Lys Val Glu Phe Glu Pro Phe Gln Ser Leu Val 180 185 190 Pro Lys Ala Ala Arg Gly Gln Tyr Ser Gly Phe Val Arg Thr Leu Phe 195 200 205 Gln Gln Met Arg Asp Val Leu Gly Thr Phe Asp Thr Val Gln Ile Ile 210 215 220 Lys Leu Leu Pro Phe Ala Ala Ala Pro Pro Glu Gln Ser Arg Met Gln 225 230 235 240 Phe Ser Ser Leu Thr Val Asn Val Arg Gly Ser Gly Met Arg Ile Leu 245 250 255 Val Arg Gly Asn Ser Pro Val Phe Asn Tyr Asn Lys Ala Ile Lys Arg 260 265 270 Leu Ile Val Leu Gly Lys Asp Ala Gly Ala Leu Thr Glu Asp Pro Asp 275 280 285 Glu Gly Thr Ala Gly Val Glu Ser Ala Val Leu Arg Gly Phe Leu Ile 290 295 300 Leu Gly Lys Glu Asp Lys Arg Tyr Gly Pro Ala Leu Ser Ile Asn Glu 305 310 315 320 Leu Ser Asn Leu Ala Lys Gly Glu Lys Ala Asn Val Leu Ile Gly Gln 325 330 335 Gly Asp Val Val Leu Val Met Lys Arg Lys Arg Asp Ser Gly Ile Leu 340 345 350 Thr Asp Ser Gln Thr Ala Thr Lys Arg Ile Arg Met Ala Ile 355 360 365 <210> 50 <211> 757 <212> PRT <213> PB1 of A/swine/Korea/S81/2004(H9N2) <400> 50 Met Asp Val Asn Pro Thr Leu Leu Phe Leu Lys Val Pro Ala Gln Asn 1 5 10 15 Ala Ile Ser Thr Thr Phe Pro Tyr Thr Gly Asp Pro Pro Tyr Ser His 20 25 30 Gly Thr Gly Thr Gly Tyr Thr Met Asp Thr Val Asn Arg Thr His Gln 35 40 45 Tyr Ser Glu Arg Gly Arg Trp Thr Thr Asn Thr Glu Thr Gly Ala Pro 50 55 60 Gln Leu Asn Pro Ile Asp Gly Pro Leu Pro Glu Asp Asn Glu Pro Ser 65 70 75 80 Gly Tyr Ala Gln Thr Asp Arg Val Leu Glu Ala Met Ala Phe Leu Glu 85 90 95 Glu Ser His Pro Gly Ile Phe Glu Thr Ser Cys Leu Glu Thr Met Glu 100 105 110 Val Val Gln Gln Thr Arg Val Asp Lys Leu Thr Gln Gly Arg Gln Thr 115 120 125 Tyr Asp Trp Thr Leu Asn Arg Asn Gln Pro Ala Ala Thr Ala Leu Ala 130 135 140 Asn Thr Ile Glu Val Phe Arg Ser Asn Gly Leu Thr Ala Asn Glu Ser 145 150 155 160 Gly Arg Leu Ile Asp Phe Leu Lys Asp Val Met Glu Ser Met Asn Lys 165 170 175 Glu Glu Met Glu Ile Thr Thr His Phe Gln Arg Lys Arg Arg Val Arg 180 185 190 Asp Asn Met Thr Lys Lys Met Val Thr Gln Arg Thr Ile Gly Lys Arg 195 200 205 Lys Gln Arg Leu Asn Lys Gly Ser Tyr Leu Ile Arg Ala Leu Thr Leu 210 215 220 Asn Thr Met Thr Lys Asp Ala Glu Arg Gly Lys Leu Lys Arg Arg Ala 225 230 235 240 Ile Ala Thr Pro Gly Met Gln Ile Arg Gly Phe Val Tyr Phe Val Glu 245 250 255 Thr Leu Ala Arg Ser Ile Cys Glu Lys Leu Glu Gln Ser Gly Leu Pro 260 265 270 Val Gly Gly Asn Glu Lys Lys Ala Lys Leu Ala Asn Val Val Arg Lys 275 280 285 Met Met Thr Asn Ser Gln Asp Thr Glu Ile Ser Phe Thr Ile Thr Gly 290 295 300 Asp Asn Thr Lys Trp Asn Glu Asn Gln Asn Pro Arg Met Phe Leu Ala 305 310 315 320 Met Ile Thr Tyr Ile Thr Arg Asn Gln Pro Glu Trp Phe Arg Asn Val 325 330 335 Leu Ser Ile Ala Pro Ile Met Phe Ser Asn Lys Met Ala Arg Leu Gly 340 345 350 Lys Gly Tyr Met Phe Glu Ser Lys Ser Met Lys Ile Arg Thr Gln Ile 355 360 365 Pro Ala Glu Met Leu Ala Ser Ile Asp Leu Lys Tyr Phe Asn Asp Ser 370 375 380 Thr Arg Lys Lys Ile Glu Lys Ile Arg Pro Leu Leu Ile Asp Gly Thr 385 390 395 400 Ala Ser Leu Ser Pro Gly Met Met Met Gly Met Phe Asn Met Leu Ser 405 410 415 Thr Val Leu Gly Val Ser Ile Leu Asn Leu Gly Gln Lys Arg His Thr 420 425 430 Lys Thr Thr Tyr Trp Trp Asp Gly Leu Gln Ser Ser Asp Asp Phe Ala 435 440 445 Leu Ile Val Asn Ala Pro Asn His Glu Gly Ile Gln Ala Gly Val Asn 450 455 460 Arg Phe Tyr Arg Thr Cys Lys Leu Leu Gly Ile Asn Met Ser Lys Lys 465 470 475 480 Lys Ser Tyr Ile Asn Arg Thr Gly Thr Phe Glu Phe Thr Ser Phe Phe 485 490 495 Tyr Arg Tyr Gly Phe Val Ala Asn Phe Ser Met Glu Leu Pro Ser Phe 500 505 510 Gly Val Ser Gly Ile Asn Glu Ser Ala Asp Met Ser Ile Gly Val Thr 515 520 525 Val Ile Lys Asn Asn Met Ile Asn Asn Asp Leu Gly Pro Ala Thr Ala 530 535 540 Gln Met Ala Leu Gln Leu Phe Ile Lys Asp Tyr Arg Tyr Thr Tyr Arg 545 550 555 560 Cys His Arg Gly Asp Thr Gln Ile Gln Thr Arg Arg Ser Phe Glu Ile 565 570 575 Lys Lys Leu Trp Glu Gln Thr His Ser Lys Ala Gly Leu Leu Val Ser 580 585 590 Asp Gly Gly Pro Asn Leu Tyr Asn Ile Arg Asn Leu His Ile Pro Glu 595 600 605 Val Cys Leu Lys Trp Glu Leu Met Asp Glu Asp Tyr Gln Gly Arg Leu 610 615 620 Cys Asn Pro Leu Asn Pro Phe Val Asn His Lys Asp Ile Glu Ser Val 625 630 635 640 Asn Asn Ala Val Ile Met Pro Ala His Gly Pro Ala Lys Asn Met Glu 645 650 655 Tyr Asp Ala Val Ala Thr Thr His Ser Trp Ile Pro Lys Arg Asn Arg 660 665 670 Ser Ile Leu Asn Thr Ser Gln Arg Gly Ile Leu Glu Asp Glu Gln Met 675 680 685 Tyr Gln Lys Cys Cys Asn Leu Phe Glu Lys Phe Phe Pro Ser Ser Ser 690 695 700 Tyr Arg Arg Pro Val Gly Ile Ser Ser Met Val Glu Ala Met Val Ser 705 710 715 720 Arg Ala Arg Ile Asp Ala Arg Ile Asp Phe Glu Ser Gly Arg Ile Lys 725 730 735 Arg Glu Glu Phe Thr Glu Ile Met Lys Ile Cys Ser Thr Ile Glu Glu 740 745 750 Leu Arg Arg Gln Lys 755 <210> 51 <211> 716 <212> PRT <213> PA of A/swine/Korea/S81/2004(H9N2) <400> 51 Met Glu Asp Phe Val Arg Gln Trp Leu Gln Ser Asp Ile Val Glu Leu 1 5 10 15 Ala Glu Lys Ala Met Lys Glu Tyr Gly Glu Asp Leu Lys Ile Glu Thr 20 25 30 Asn Lys Phe Ala Ala Ile Cys Thr His Leu Glu Val Cys Phe Met Tyr 35 40 45 Ser Asp Phe His Phe Thr Asp Glu Gln Gly Glu Ser Ile Val Val Glu 50 55 60 Leu Gly Asp Pro Asn Ala Leu Leu Lys His Arg Phe Glu Ile Ile Glu 65 70 75 80 Gly Arg Asp Arg Thr Ile Ala Trp Thr Val Ile Asn Ser Ile Cys Asn 85 90 95 Thr Thr Gly Ala Glu Lys Pro Lys Phe Leu Pro Asp Leu Tyr Asp Tyr 100 105 110 Lys Lys Asn Arg Phe Ile Glu Ile Gly Val Thr Arg Arg Glu Val His 115 120 125 Ile Tyr Tyr Leu Glu Lys Ala Asn Lys Ile Lys Ser Glu Lys Thr His 130 135 140 Ile His Ile Phe Ser Phe Thr Gly Glu Glu Met Ala Thr Lys Ala Asp 145 150 155 160 Tyr Thr Leu Asp Glu Glu Ser Arg Ala Arg Ile Lys Thr Arg Leu Phe 165 170 175 Thr Ile Arg Gln Glu Met Ala Ser Arg Gly Leu Trp Asp Ser Phe Arg 180 185 190 Gln Ser Glu Arg Gly Glu Glu Thr Ile Glu Glu Arg Phe Glu Ile Thr 195 200 205 Gly Thr Met Arg Lys Leu Ala Asp Gln Ser Leu Pro Pro Asn Phe Ser 210 215 220 Ser Leu Glu Lys Phe Arg Ala Tyr Val Asp Gly Phe Glu Pro Asn Gly 225 230 235 240 Tyr Ile Glu Gly Lys Leu Ser Gln Met Ser Lys Glu Val Asn Ala Arg 245 250 255 Ile Glu Pro Phe Leu Lys Ser Thr Pro Arg Pro Leu Arg Leu Pro Asp 260 265 270 Gly Pro Pro Cys Ser Gln Arg Ser Lys Phe Leu Leu Met Asp Ala Leu 275 280 285 Lys Leu Ser Ile Glu Asp Pro Ser His Glu Gly Glu Gly Ile Pro Leu 290 295 300 Tyr Asp Ala Ile Lys Cys Met Arg Thr Phe Phe Gly Trp Lys Glu Pro 305 310 315 320 Asn Val Val Lys Pro His Glu Lys Gly Ile Asn Pro Asn Tyr Leu Leu 325 330 335 Thr Trp Lys Gln Val Leu Ala Glu Leu Gln Asp Ile Glu Asn Glu Glu 340 345 350 Lys Ile Pro Arg Thr Lys Asn Met Lys Lys Thr Ser Gln Leu Lys Trp 355 360 365 Ala Leu Gly Glu Asn Val Ala Leu Glu Lys Val Asp Phe Asp Asp Cys 370 375 380 Lys Asp Val Gly Asp Leu Lys Gln Tyr Asp Ser Asp Glu Pro Glu Leu 385 390 395 400 Arg Ser Leu Ala Ser Trp Ile Gln Asn Glu Phe Asn Lys Ala Cys Glu 405 410 415 Leu Thr Asp Ser Ser Arg Ile Glu Leu Asp Glu Ile Gly Glu Asp Ala 420 425 430 Ala Pro Ile Glu His Ile Ala Ser Met Arg Arg Asn Tyr Phe Thr Ala 435 440 445 Glu Val Ser His Cys Arg Ala Thr Glu Tyr Ile Met Lys Gly Val Tyr 450 455 460 Ile Asn Thr Ala Val Leu Asn Ala Ser Cys Ala Ala Met Asp Gly Phe 465 470 475 480 Gln Leu Ile Pro Met Ile Ser Lys Cys Arg Thr Lys Glu Gly Arg Arg 485 490 495 Lys Thr Asn Leu Tyr Gly Phe Ile Ile Lys Gly Arg Ser His Leu Arg 500 505 510 Asn Asp Thr Asp Val Val Asn Phe Val Ser Met Glu Phe Ser Leu Thr 515 520 525 Asp Pro Arg Leu Glu Pro His Lys Trp Glu Lys Tyr Cys Val Leu Glu 530 535 540 Val Gly Asp Met Leu Leu Arg Ser Ala Ile Gly His Val Ser Arg Pro 545 550 555 560 Met Phe Leu Tyr Val Arg Thr Asn Gly Thr Ser Lys Ile Lys Met Lys 565 570 575 Trp Gly Met Glu Met Arg Arg Cys Leu Leu Gln Ser Leu Gln Gln Ile 580 585 590 Glu Ser Met Ile Glu Ala Glu Ser Ser Val Lys Glu Lys Asp Met Thr 595 600 605 Lys Glu Phe Phe Glu Asn Lys Ser Glu Thr Trp Pro Val Gly Glu Ser 610 615 620 Pro Lys Gly Val Glu Glu Gly Ser Ile Gly Lys Val Cys Arg Thr Leu 625 630 635 640 Leu Ala Lys Ser Val Phe Asn Ser Leu Tyr Ala Ser Pro Gln Leu Glu 645 650 655 Gly Phe Ser Ala Glu Ser Arg Lys Leu Leu Leu Ile Val Gln Ala Leu 660 665 670 Arg Asp Asn Leu Glu Pro Gly Thr Phe Asp Leu Gly Gly Leu Tyr Glu 675 680 685 Ala Ile Glu Glu Cys Leu Ile Asn Asp Pro Trp Val Leu Leu Asn Ala 690 695 700 Ser Trp Phe Asn Ser Phe Leu Thr His Ala Leu Arg 705 710 715 <210> 52 <211> 368 <212> PRT <213> HA of A/swine/Korea/S81/2004(H9N2) <400> 52 Met Glu Val Ile Ala Leu Ile Ala Ile Leu Val Val Thr Gly Thr Ser 1 5 10 15 Asn Ala Asp Lys Ile Cys Ile Gly Tyr Gln Ser Thr Asn Ser Thr Glu 20 25 30 Thr Val Asp Thr Leu Val Glu Thr Asn Val Pro Val Thr His Ala Lys 35 40 45 Glu Leu Leu His Thr Glu His Asn Gly Met Leu Cys Ala Thr Asn Gln 50 55 60 Gly Arg Pro Leu Ile Leu Asp Thr Cys Thr Val Glu Gly Leu Val Tyr 65 70 75 80 Gly Asn Pro Ser Cys Asp Leu Leu Leu Gly Gly Lys Glu Trp Ser Tyr 85 90 95 Ile Val Glu Arg Ser Ser Ala Ile Asn Gly Met Cys Tyr Pro Gly Arg 100 105 110 Val Glu Asn Leu Glu Glu Leu Arg Ser Phe Phe Ser Ser Ala Arg Ser 115 120 125 Tyr Lys Arg Leu Leu Leu Phe Pro Asp Arg Thr Trp Asn Val Thr Tyr 130 135 140 Thr Gly Thr Ser Lys Ala Cys Ser Gly Ser Phe Tyr Arg Ser Met Arg 145 150 155 160 Trp Leu Thr His Lys Ser Asn Ser Tyr Pro Ile Gln Asp Ala Gln Tyr 165 170 175 Thr Asn Asp Trp Gly Lys Asn Ile Leu Phe Met Trp Gly Ile His His 180 185 190 Pro Pro Thr Asp Thr Glu Gln Met Asn Leu Tyr Lys Lys Ala Asp Thr 195 200 205 Thr Thr Ser Ile Thr Thr Glu Asp Ile Asn Arg Thr Phe Lys Pro Val 210 215 220 Ile Gly Pro Arg Pro Leu Val Asn Gly Gln Gln Gly Arg Ile Asp Tyr 225 230 235 240 Tyr Trp Ser Val Leu Lys Pro Gly Gln Thr Leu Arg Ile Arg Ser Asn 245 250 255 Gly Asn Leu Ile Ala Pro Trp Tyr Gly His Ile Leu Ser Gly Glu Ser 260 265 270 His Gly Arg Ile Leu Lys Thr Asp Leu Asn Ser Gly Asn Cys Val Val 275 280 285 Gln Cys Gln Thr Glu Lys Gly Gly Leu Asn Thr Thr Leu Pro Phe His 290 295 300 Asn Val Ser Lys Tyr Ala Phe Gly Asn Cys Pro Lys Tyr Val Gly Val 305 310 315 320 Lys Ser Leu Lys Leu Ala Val Gly Leu Arg Asn Val Pro Ala Ala Ser 325 330 335 Gly Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp 340 345 350 Pro Gly Leu Val Ala Gly Trp Tyr Gly Phe Gln His Ser Asn Asp Gln 355 360 365 <210> 53 <211> 180 <212> PRT <213> NP of A/swine/Korea/S81/2004(H9N2) <400> 53 Glu Thr Asp Gly Glu Arg Gln Asn Ala Thr Glu Ile Arg Ala Ser Val 1 5 10 15 Gly Lys Met Ile Asp Gly Ile Gly Arg Phe Tyr Ile Gln Met Cys Thr 20 25 30 Glu Leu Lys Leu Ser Asp Tyr Glu Gly Arg Leu Ile Gln Asn Ser Leu 35 40 45 Thr Ile Glu Arg Met Val Leu Ser Ala Phe Asp Glu Arg Arg Asn Lys 50 55 60 Tyr Leu Glu Glu His Pro Ser Ala Gly Lys Asp Pro Lys Lys Thr Gly 65 70 75 80 Gly Pro Ile Tyr Arg Arg Val Asp Gly Lys Trp Arg Arg Glu Leu Ile 85 90 95 Leu Tyr Asp Lys Glu Glu Ile Arg Arg Ile Trp Arg Gln Ala Asn Asn 100 105 110 Gly Asp Asp Ala Thr Ala Gly Leu Thr His Met Met Ile Trp His Ser 115 120 125 Asn Leu Asn Asp Ala Thr Tyr Gln Arg Thr Lys Ala Leu Val Arg Thr 130 135 140 Gly Met Asp Pro Arg Met Cys Ser Leu Met Gln Gly Ser Thr Leu Pro 145 150 155 160 Arg Arg Ser Gly Ala Ala Gly Ala Ala Val Lys Gly Val Gly Thr Met 165 170 175 Val Met Glu Leu 180 <210> 54 <211> 469 <212> PRT <213> NA of A/swine/Korea/S81/2004(H9N2) <400> 54 Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Ser Leu Thr 1 5 10 15 Ile Ala Thr Val Cys Phe Leu Met Gln Ile Ala Ile Leu Ala Thr Thr 20 25 30 Val Thr Leu His Phe Lys Gln Asn Glu Cys Ser Ile Pro Ser Asn Asn 35 40 45 Gln Ala Val Pro Cys Glu Pro Ile Ile Ile Glu Lys Asn Ile Thr Lys 50 55 60 Ile Val Tyr Leu Asn Asn Thr Thr Ile Glu Lys Glu Leu Cys Pro Lys 65 70 75 80 Val Val Glu Tyr Arg Ser Trp Ser Lys Pro Gln Cys Gln Ile Thr Gly 85 90 95 Phe Ala Pro Phe Ser Lys Asp Asn Ser Val Arg Leu Ser Ala Gly Gly 100 105 110 Ser Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Ser Ser Asn Lys 115 120 125 Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asp Asn Lys His 130 135 140 Ser Asn Gly Thr Ile His Asp Arg Ile Pro His Arg Thr Leu Leu Met 145 150 155 160 Asn Glu Leu Gly Val Pro Phe His Leu Gly Thr Lys Gln Val Cys Ile 165 170 175 Ala Trp Ser Ser Ser Ser Cys His Asp Gly Lys Ala Trp Leu His Val 180 185 190 Cys Val Thr Gly Asp Asp Arg Asn Ala Thr Ala Ser Phe Ile Tyr Asp 195 200 205 Gly Met Leu Val Asp Ser Ile Gly Ser Trp Ser Gln Asn Ile Leu Arg 210 215 220 Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Thr Cys Thr Val Val 225 230 235 240 Met Thr Asp Gly Ser Ala Ser Gly Arg Ala Asp Thr Arg Val Leu Phe 245 250 255 Ile Lys Glu Gly Lys Ile Val His Ile Ser Gln Leu Ser Gly Ser Ala 260 265 270 Gln His Ile Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Asp Val Arg 275 280 285 Cys Val Tyr Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Ile Ile Asp 290 295 300 Ile Lys Met Ala Asp Tyr Ser Ile Asp Ser Ser Tyr Val Cys Ser Gly 305 310 315 320 Leu Val Gly Asp Thr Pro Arg Asp Asp Asp Ser Ser Ser Asn Ser Asn 325 330 335 Cys Lys Asp Pro Asn Asn Glu Arg Gly Asn Pro Gly Val Lys Gly Trp 340 345 350 Ala Phe Asp Tyr Gly Asn Asp Val Trp Met Gly Arg Thr Ile Ser Lys 355 360 365 Asp Ser Arg Ser Gly Tyr Glu Thr Phe Lys Val Ile Asp Gly Trp Thr 370 375 380 Thr Ala Asn Ser Lys Ser Gln Val Asn Arg Gln Val Ile Val Gly Asn 385 390 395 400 Asn Asn Trp Ser Gly Tyr Ser Gly Ile Phe Ser Val Glu Gly Lys Ser 405 410 415 Cys Val Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Pro Gln 420 425 430 Glu Thr Arg Val Trp Trp Thr Ser Asn Ser Ile Val Val Phe Cys Gly 435 440 445 Thr Ser Gly Thr Tyr Gly Thr Gly Ser Trp Pro Asp Gly Ala Asn Ile 450 455 460 Asn Phe Met Pro Ile 465 <210> 55 <211> 252 <212> PRT <213> M of A/swine/Korea/S81/2004(H9N2) <400> 55 Met Ser Leu Leu Thr Glu Val Glu Thr Tyr Val Leu Ser Ile Val Pro 1 5 10 15 Ser Gly Pro Leu Lys Ala Glu Ile Ala Gln Arg Leu Glu Asp Val Phe 20 25 30 Ala Gly Lys Asn Thr Asp Leu Glu Val Leu Met Glu Trp Leu Lys Thr 35 40 45 Arg Pro Ile Leu Ser Pro Leu Thr Lys Gly Ile Leu Gly Phe Val Phe 50 55 60 Thr Leu Thr Val Pro Ser Glu Arg Gly Leu Gln Cys Arg Arg Phe Val 65 70 75 80 Gln Asn Ala Leu Asn Gly Asn Gly Asp Pro Asn Asn Met Asp Lys Ala 85 90 95 Val Lys Leu Tyr Arg Lys Leu Lys Arg Glu Ile Thr Phe His Gly Ala 100 105 110 Lys Glu Ile Ala Leu Ser Tyr Ser Ala Gly Ala Leu Ala Cys Cys Met 115 120 125 Gly Leu Ile Tyr Asn Arg Met Gly Ala Val Thr Thr Glu Val Ala Phe 130 135 140 Gly Leu Val Cys Ala Thr Cys Glu Gln Ile Ala Asp Ser Gln His Arg 145 150 155 160 Ser His Arg Gln Met Val Thr Thr Thr Asn Pro Leu Ile Arg His Glu 165 170 175 Asn Arg Met Val Leu Ala Ser Thr Thr Ala Lys Ala Met Glu Gln Met 180 185 190 Ala Gly Ser Ser Glu Gln Ala Ala Glu Ala Met Asp Ile Ala Ser Gln 195 200 205 Ala Arg Gln Met Val Gln Ala Met Arg Thr Val Gly Thr His Pro Ser 210 215 220 Ser Ser Ala Gly Leu Lys Asp Asp Leu Leu Glu Asn Leu Gln Ala Tyr 225 230 235 240 Gln Lys Arg Met Gly Val Gln Met Gln Arg Phe Lys 245 250 <210> 56 <211> 230 <212> PRT <213> NS of A/swine/Korea/S81/2004(H9N2) <400> 56 Met Asp Ser Asn Thr Val Ser Ser Phe Gln Val Asp Cys Phe Leu Trp 1 5 10 15 His Val Arg Lys Arg Phe Ala Asp Gln Glu Leu Gly Asp Ala Pro Phe 20 25 30 Leu Asp Arg Leu Arg Arg Asp Gln Lys Ser Leu Arg Gly Arg Gly Asn 35 40 45 Thr Leu Gly Leu Asp Ile Glu Thr Ala Thr His Ala Gly Lys Arg Ile 50 55 60 Val Glu Arg Ile Leu Glu Glu Glu Pro Asp Glu Ala Leu Lys Met Ala 65 70 75 80 Val Ala Ser Val Pro Ala Ser Arg Tyr Leu Ala Asp Met Thr Leu Glu 85 90 95 Glu Met Ser Arg Asp Trp Phe Met Leu Met Pro Lys Gln Lys Val Ala 100 105 110 Gly Ser Leu Cys Ile Lys Met Asp Gln Ala Ile Met Asp Lys Asn Ile 115 120 125 Thr Leu Lys Ala Asn Phe Ser Val Ile Phe Asn Arg Leu Glu Thr Leu 130 135 140 Ile Leu Leu Arg Ala Phe Thr Glu Glu Gly Val Ile Val Gly Glu Ile 145 150 155 160 Ser Pro Leu Pro Ser Leu Pro Gly His Thr Asn Glu Asp Val Lys Asn 165 170 175 Ala Ile Gly Ala Leu Ile Gly Gly Leu Glu Trp Asn Asp Asn Thr Val 180 185 190 Arg Val Ser Glu Thr Leu Gln Arg Leu Thr Trp Arg Ser Ser Asp Glu 195 200 205 Asn Gly Gly Pro Ser Leu Pro Pro Lys Gln Lys Arg Lys Met Ala Arg 210 215 220 Thr Ile Glu Ser Glu Ile 225 230 <210> 57 <211> 345 <212> PRT <213> PB2 of A/swine/Korea/S83/2004(H9N2) <400> 57 Val Arg Gly Asp Leu Asn Phe Val Asn Arg Ala Asn Gln Arg Leu Asn 1 5 10 15 Pro Ile His Gln Leu Leu Arg His Phe Gln Lys Asp Ala Lys Val Leu 20 25 30 Phe Gln Asn Trp Gly Ile Glu Pro Ile Asp Asn Val Met Gly Met Ile 35 40 45 Gly Val Leu Pro Asp Met Thr Pro Ser Thr Glu Met Ser Leu Arg Gly 50 55 60 Val Arg Val Ser Lys Met Gly Val Asp Glu Tyr Ser Ser Thr Glu Arg 65 70 75 80 Val Val Val Ser Ile Asp Arg Phe Leu Arg Val Arg Asp Gln Arg Gly 85 90 95 Asn Ile Leu Leu Ser Pro Glu Glu Val Ser Glu Thr Gln Gly Thr Glu 100 105 110 Lys Leu Thr Ile Thr Tyr Ser Ser Ser Met Met Trp Glu Ile Asn Gly 115 120 125 Pro Glu Ser Val Leu Val Asn Thr Tyr Gln Trp Ile Ile Arg Asn Trp 130 135 140 Glu Thr Val Lys Ile Gln Trp Ser Gln Asp Pro Thr Met Leu Tyr Asn 145 150 155 160 Lys Val Glu Phe Glu Pro Phe Gln Ser Leu Val Pro Lys Ala Ala Arg 165 170 175 Gly Gln Tyr Ser Gly Phe Val Arg Thr Leu Phe Gln Gln Met Arg Asp 180 185 190 Val Leu Gly Thr Phe Asp Thr Val Gln Ile Ile Lys Leu Leu Pro Phe 195 200 205 Ala Ala Ala Pro Pro Glu Gln Ser Arg Met Gln Phe Ser Ser Leu Thr 210 215 220 Val Asn Ala Arg Gly Ser Gly Met Arg Ile Leu Val Arg Gly Asn Ser 225 230 235 240 Pro Val Phe Asn Tyr Asn Lys Ala Ile Lys Arg Leu Thr Val Leu Gly 245 250 255 Lys Asp Ala Gly Ala Leu Thr Glu Asp Pro Asp Glu Gly Thr Ala Gly 260 265 270 Val Glu Thr Ala Val Leu Arg Gly Phe Leu Ile Leu Gly Lys Glu Asp 275 280 285 Lys Arg Tyr Gly Pro Ala Leu Ser Ile Asn Glu Leu Ser Asn Leu Ala 290 295 300 Lys Gly Glu Lys Ala Asn Val Leu Ile Gly Gln Gly Asp Val Val Leu 305 310 315 320 Val Met Lys Arg Lys Arg Asp Ser Ser Ile Leu Thr Asp Ser Gln Thr 325 330 335 Ala Ile Glu Arg Ile Arg Met Ala Ile 340 345 <210> 58 <211> 375 <212> PRT <213> PB1 of A/swine/Korea/S83/2004(H9N2) <400> 58 Asp Ser Thr Arg Lys Lys Ile Glu Lys Ile Arg Pro Leu Leu Ile Asp 1 5 10 15 Gly Thr Ala Ser Leu Ser Pro Gly Met Met Met Gly Met Phe Asn Met 20 25 30 Leu Ser Thr Val Leu Gly Val Ser Ile Leu Asn Leu Gly Gln Lys Arg 35 40 45 His Thr Lys Thr Thr Tyr Trp Trp Asp Gly Leu Gln Ser Ser Asp Asp 50 55 60 Phe Ala Leu Ile Val Asn Ala Pro Asn His Glu Gly Ile Gln Ala Gly 65 70 75 80 Val Asn Arg Phe Tyr Arg Thr Cys Lys Leu Leu Gly Ile Asn Met Ser 85 90 95 Lys Lys Lys Ser His Ile Asn Arg Thr Gly Thr Phe Glu Phe Thr Ser 100 105 110 Phe Phe Tyr Arg Tyr Gly Phe Val Ala Asn Phe Ser Met Glu Leu Pro 115 120 125 Ser Phe Gly Val Ser Gly Ile Asn Glu Ser Ala Asp Ala Ser Ile Gly 130 135 140 Val Thr Val Ile Lys Asn Asn Met Ile Asn Asn Asp Leu Gly Pro Ala 145 150 155 160 Thr Ala Gln Met Ala Leu Gln Leu Phe Ile Lys Asp Tyr Arg Tyr Thr 165 170 175 Tyr Arg Cys His Arg Gly Asp Thr Gln Ile Gln Thr Arg Arg Ser Phe 180 185 190 Glu Ile Lys Lys Leu Trp Glu Gln Thr His Ser Lys Ala Gly Leu Leu 195 200 205 Val Ser Asp Gly Gly Pro Asn Leu Tyr Asn Ile Arg Asn Leu His Ile 210 215 220 Pro Glu Val Cys Leu Lys Trp Glu Leu Met Asp Glu Asp Tyr Gln Gly 225 230 235 240 Arg Leu Cys Asn Pro Leu Asn Pro Phe Val Asn His Lys Asp Ile Glu 245 250 255 Ser Val Asn Asn Ala Val Ile Val Pro Ala His Gly Pro Ala Lys Asn 260 265 270 Met Glu Tyr Asp Ala Val Ala Thr Thr His Ser Trp Ile Pro Lys Arg 275 280 285 Asn Arg Ser Ile Leu Asn Thr Ser Gln Arg Gly Ile Leu Glu Asp Glu 290 295 300 Gln Met Tyr Gln Lys Cys Cys Asn Leu Phe Glu Lys Phe Phe Pro Ser 305 310 315 320 Ser Ser Tyr Arg Arg Pro Val Gly Ile Ser Ser Met Val Glu Ala Met 325 330 335 Val Ser Arg Ala Arg Ile Asp Ala Arg Ile Asp Phe Glu Ser Gly Arg 340 345 350 Ile Lys Lys Glu Glu Phe Thr Glu Ile Met Lys Ile Cys Ser Thr Ile 355 360 365 Glu Glu Leu Arg Arg Gln Lys 370 375 <210> 59 <211> 716 <212> PRT <213> PA of A/swine/Korea/S83/2004(H9N2) <400> 59 Met Glu Asp Phe Val Arg Gln Cys Phe Asn Pro Met Ile Val Glu Leu 1 5 10 15 Ala Glu Lys Ala Met Lys Glu Tyr Gly Glu Asp Leu Lys Ile Glu Thr 20 25 30 Asn Lys Phe Ala Ala Ile Cys Thr His Leu Glu Val Cys Phe Met Tyr 35 40 45 Ser Asp Phe His Phe Ile Asp Glu Gln Gly Glu Ser Ile Val Val Glu 50 55 60 Leu Gly Asp Pro Asn Ala Leu Leu Lys His Arg Phe Glu Ile Ile Glu 65 70 75 80 Gly Arg Asp Arg Thr Ile Ala Trp Thr Val Ile Asn Ser Ile Cys Asn 85 90 95 Thr Thr Gly Ala Glu Lys Pro Lys Phe Leu Pro Asp Leu Tyr Asp Tyr 100 105 110 Lys Lys Asn Arg Phe Ile Glu Ile Gly Val Thr Arg Arg Glu Val His 115 120 125 Ile Tyr Tyr Leu Glu Lys Ala Asn Lys Ile Lys Ser Glu Lys Thr His 130 135 140 Ile His Ile Phe Ser Phe Thr Gly Glu Glu Met Ala Thr Lys Ala Asp 145 150 155 160 Tyr Thr Leu Asp Glu Glu Ser Arg Ala Arg Ile Lys Thr Arg Leu Phe 165 170 175 Thr Ile Arg Gln Glu Met Ala Ser Arg Gly Leu Trp Asp Ser Phe Arg 180 185 190 Gln Ser Glu Arg Gly Glu Glu Ala Ile Glu Glu Arg Phe Glu Ile Thr 195 200 205 Gly Thr Met Arg Lys Leu Ala Asp Gln Ser Leu Pro Pro Asn Phe Ser 210 215 220 Ser Leu Glu Lys Phe Arg Ala Tyr Val Asp Gly Phe Glu Pro Asn Gly 225 230 235 240 Tyr Ile Glu Gly Lys Leu Ser Gln Met Ser Lys Glu Val Asn Ala Arg 245 250 255 Ile Glu Pro Phe Leu Lys Ser Thr Pro Arg Pro Leu Arg Leu Pro Asp 260 265 270 Gly Pro Pro Cys Ser Gln Arg Ser Lys Phe Leu Arg Met Asp Ala Leu 275 280 285 Lys Leu Ser Ile Glu Asp Pro Ser His Glu Gly Glu Gly Ile Pro Leu 290 295 300 Tyr Asp Ala Ile Lys Cys Met Arg Thr Phe Phe Gly Trp Lys Glu Pro 305 310 315 320 Asn Val Val Lys Pro His Glu Lys Gly Ile Asn Pro Asn Tyr Leu Leu 325 330 335 Ser Trp Lys Gln Val Leu Ala Glu Leu Gln Asp Ile Glu Asn Glu Glu 340 345 350 Lys Ile Pro Arg Thr Lys Asn Met Lys Lys Thr Ser Gln Leu Lys Trp 355 360 365 Ala Leu Gly Glu Asn Met Ala Pro Glu Lys Val Asp Phe Asp Gly Cys 370 375 380 Lys Asp Ile Gly Asp Leu Lys Gln Tyr Asp Ser Asp Glu Pro Glu Leu 385 390 395 400 Arg Ser Leu Ala Ser Trp Ile Gln Asn Glu Phe Asn Lys Ala Cys Glu 405 410 415 Leu Thr Asp Ser Ser Trp Ile Glu Leu Asp Glu Ile Gly Glu Asp Ala 420 425 430 Ala Pro Ile Glu His Ile Ala Ser Met Arg Arg Asn Tyr Phe Thr Ala 435 440 445 Glu Val Ser His Cys Arg Ala Thr Glu Tyr Ile Met Lys Gly Val Tyr 450 455 460 Ile Asn Thr Ala Leu Leu Asn Ala Ser Cys Ala Ala Met Asp Asp Phe 465 470 475 480 Gln Leu Ile Pro Met Ile Ser Lys Cys Arg Thr Lys Glu Gly Arg Arg 485 490 495 Lys Thr Asn Leu Tyr Gly Phe Ile Ile Lys Gly Arg Ser His Leu Arg 500 505 510 Asn Asp Thr Asp Val Val Asn Phe Val Ser Met Glu Phe Ser Leu Thr 515 520 525 Asp Pro Arg Leu Glu Pro His Lys Trp Glu Lys Tyr Cys Val Leu Glu 530 535 540 Val Gly Asp Met Leu Leu Arg Ser Ala Ile Gly His Val Ser Arg Pro 545 550 555 560 Met Phe Leu Tyr Val Arg Thr Asn Gly Thr Ser Lys Ile Lys Met Lys 565 570 575 Trp Gly Met Glu Met Arg Arg Cys Leu Leu Gln Ser Leu Gln Gln Ile 580 585 590 Glu Ser Met Ile Glu Ala Glu Ser Ser Val Lys Glu Lys Asp Met Thr 595 600 605 Lys Glu Phe Phe Glu Asn Lys Ser Glu Thr Trp Pro Val Gly Glu Ser 610 615 620 Pro Lys Gly Val Glu Glu Gly Ser Ile Gly Lys Val Cys Arg Thr Leu 625 630 635 640 Leu Ala Lys Ser Val Phe Asn Ser Leu Tyr Ala Ser Pro Gln Leu Glu 645 650 655 Gly Phe Ser Ala Glu Ser Arg Lys Leu Leu Leu Ile Val Gln Ala Leu 660 665 670 Arg Asp Asn Leu Glu Pro Gly Thr Phe Asp Leu Gly Gly Leu Tyr Glu 675 680 685 Ala Ile Glu Glu Cys Leu Ile Asn Asp Pro Trp Val Leu Leu Asn Ala 690 695 700 Ser Trp Phe Asn Ser Phe Leu Thr His Ala Leu Arg 705 710 715 <210> 60 <211> 368 <212> PRT <213> HA of A/swine/Korea/S83/2004(H9N2) <400> 60 Met Glu Val Ile Ala Leu Ile Ala Ile Leu Val Val Thr Gly Thr Ser 1 5 10 15 Ser Ala Asp Lys Ile Cys Ile Gly Tyr Gln Ser Thr Asn Ser Thr Glu 20 25 30 Thr Val Asp Thr Leu Val Glu Thr Asn Val Pro Val Thr His Ala Lys 35 40 45 Glu Leu Leu His Thr Glu His Asn Gly Met Leu Cys Ala Thr Asn Gln 50 55 60 Gly Arg Pro Leu Ile Leu Asp Thr Cys Thr Val Glu Gly Leu Val Tyr 65 70 75 80 Gly Asn Pro Ser Arg Asp Leu Leu Leu Gly Gly Lys Glu Trp Ser Tyr 85 90 95 Ile Val Lys Arg Ser Ser Ala Ile Asn Gly Met Cys Tyr Pro Gly Arg 100 105 110 Val Glu Asn Leu Glu Glu Leu Arg Ser Phe Phe Ser Ser Ala Arg Ser 115 120 125 Tyr Lys Arg Leu Leu Leu Phe Pro Asp Arg Thr Trp Asn Val Thr Tyr 130 135 140 Thr Gly Thr Ser Lys Ala Cys Ser Gly Ser Phe Tyr Arg Ser Met Arg 145 150 155 160 Trp Leu Thr His Lys Ser Asn Ser Tyr Pro Ile Gln Asp Ala Gln Tyr 165 170 175 Thr Asn Asp Trp Gly Lys Asn Ile Leu Phe Met Trp Gly Ile His His 180 185 190 Pro Pro Thr Asp Thr Glu Gln Met Asn Leu Tyr Lys Lys Ala Asp Thr 195 200 205 Thr Thr Ser Ile Thr Thr Glu Asp Ile Asn Arg Thr Phe Lys Pro Val 210 215 220 Ile Gly Pro Arg Pro Leu Val Asn Gly Gln Gln Gly Arg Ile Asp Tyr 225 230 235 240 Tyr Trp Ser Val Leu Lys Pro Gly Gln Thr Leu Arg Ile Arg Ser Asn 245 250 255 Gly Asn Leu Ile Ala Pro Trp Tyr Gly His Ile Pro Ser Gly Glu Ser 260 265 270 His Gly Arg Ile Leu Lys Thr Asp Leu Asn Ser Gly Asn Cys Val Val 275 280 285 Gln Cys Gln Thr Glu Lys Gly Gly Leu Asn Thr Thr Leu Pro Phe His 290 295 300 Asn Val Ser Lys Tyr Ala Phe Gly Asn Cys Pro Lys Tyr Val Gly Val 305 310 315 320 Lys Ser Leu Lys Leu Ala Val Gly Leu Arg Asn Val Pro Ala Ala Ser 325 330 335 Gly Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp 340 345 350 Pro Gly Leu Val Ala Gly Trp Tyr Gly Phe Gln His Ser Asn Asp Gln 355 360 365 <210> 61 <211> 180 <212> PRT <213> NP of A/swine/Korea/S83/2004(H9N2) <400> 61 Glu Thr Asp Gly Glu Arg Gln Asn Ala Thr Glu Ile Arg Ala Ser Val 1 5 10 15 Gly Lys Met Ile Asp Gly Ile Gly Arg Phe Tyr Ile Gln Met Cys Thr 20 25 30 Glu Leu Lys Leu Ser Asp Tyr Glu Gly Arg Leu Ile Gln Asn Ser Leu 35 40 45 Thr Ile Glu Arg Met Val Leu Ser Ala Phe Asp Glu Arg Arg Asn Lys 50 55 60 Tyr Leu Glu Glu His Pro Ser Ala Gly Lys Asp Pro Lys Lys Thr Gly 65 70 75 80 Gly Pro Ile Tyr Arg Arg Val Asp Gly Lys Trp Arg Arg Glu Leu Ile 85 90 95 Leu Tyr Asp Lys Glu Glu Ile Arg Arg Ile Trp Arg Gln Ala Asn Asn 100 105 110 Gly Asp Asp Ala Thr Ala Gly Leu Thr His Met Met Ile Trp His Ser 115 120 125 Asn Leu Asn Asp Ala Thr Tyr Gln Arg Thr Arg Ala Leu Val Arg Thr 130 135 140 Gly Met Asp Pro Arg Met Cys Ser Leu Met Gln Gly Ser Thr Leu Pro 145 150 155 160 Arg Arg Ser Gly Ala Ala Gly Ala Ala Val Lys Gly Val Gly Thr Met 165 170 175 Val Met Glu Leu 180 <210> 62 <211> 469 <212> PRT <213> NA of A/swine/Korea/S83/2004(H9N2) <400> 62 Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Ser Leu Thr 1 5 10 15 Ile Ala Thr Val Cys Phe Leu Met Gln Ile Ala Ile Leu Ala Thr Thr 20 25 30 Val Thr Leu His Phe Lys Gln Asn Glu Gly Ser Ile Pro Ser Asn Asn 35 40 45 Gln Val Val Pro Cys Glu Pro Ile Ile Ile Glu Arg Asn Ile Thr Glu 50 55 60 Ile Val Tyr Leu Asn Asn Thr Ile Ile Glu Lys Glu Leu Cys Pro Lys 65 70 75 80 Val Val Lys Tyr Arg Ser Trp Ser Lys Pro Gln Cys Gln Ile Thr Gly 85 90 95 Phe Ala Pro Phe Ser Lys Asp Asp Ser Ile Arg Leu Ser Ala Gly Gly 100 105 110 Ser Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Ser Ser Asn Lys 115 120 125 Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asp Asn Arg His 130 135 140 Ser Asn Gly Thr Ile His Asp Arg Ile Pro His Arg Thr Leu Leu Met 145 150 155 160 Asn Glu Leu Gly Val Pro Phe His Leu Gly Thr Lys Gln Val Cys Ile 165 170 175 Ala Trp Ser Ser Ser Ser Cys His Asp Gly Lys Ala Trp Leu His Val 180 185 190 Cys Val Thr Gly Asp Asp Arg Asn Ala Thr Ala Ser Phe Ile Tyr Asp 195 200 205 Gly Thr Leu Val Asp Thr Ile Gly Ser Trp Ser Gln Asn Ile Leu Arg 210 215 220 Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Thr Cys Thr Val Val 225 230 235 240 Met Thr Asp Gly Ser Ala Ser Gly Arg Ala Asp Thr Arg Ile Leu Phe 245 250 255 Ile Lys Glu Gly Lys Ile Val His Ile Ser Gln Leu Ser Gly Ser Ala 260 265 270 Gln His Ile Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Asp Val Arg 275 280 285 Cys Val Cys Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Ile Ile Asp 290 295 300 Ile Lys Met Ala Asp Tyr Ser Ile Asp Ser Ser Tyr Val Cys Ser Gly 305 310 315 320 Leu Val Gly Asp Thr Pro Arg Asn Asp Asp Ser Ser Ser Asn Ser Asn 325 330 335 Cys Lys Asp Pro Asn Asn Glu Arg Gly Asn Pro Gly Val Lys Gly Trp 340 345 350 Ala Phe Asp Tyr Gly Asn Asp Val Trp Met Gly Arg Thr Ile Ser Lys 355 360 365 Asp Ser Arg Ser Gly Tyr Glu Thr Phe Lys Val Ile Asp Gly Trp Thr 370 375 380 Thr Ala Asn Ser Lys Ser Gln Val Asn Arg Gln Val Ile Val Asp Asn 385 390 395 400 Asn Asn Trp Ser Gly Cys Ser Gly Ile Phe Ser Val Glu Gly Lys Ser 405 410 415 Cys Val Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Pro Gln 420 425 430 Glu Thr Arg Val Trp Trp Thr Ser Asn Ser Ile Val Val Phe Cys Gly 435 440 445 Thr Ser Gly Thr Tyr Gly Thr Gly Ser Trp Pro Glu Gly Ala Asn Ile 450 455 460 Asn Phe Met Pro Ile 465 <210> 63 <211> 252 <212> PRT <213> M of A/swine/Korea/S83/2004(H9N2) <400> 63 Met Ser Leu Leu Thr Glu Val Glu Thr Tyr Val Leu Ser Ile Ile Pro 1 5 10 15 Ser Gly Pro Leu Lys Ala Glu Ile Ala Gln Gly Leu Glu Asp Val Phe 20 25 30 Ala Gly Lys Asn Thr Asp Leu Glu Ala Leu Met Glu Trp Leu Lys Thr 35 40 45 Arg Pro Ile Leu Ser Pro Leu Thr Lys Gly Ile Leu Gly Phe Val Phe 50 55 60 Thr Leu Thr Val Pro Gly Glu Arg Gly Leu Gln Arg Arg Arg Phe Val 65 70 75 80 Gln Asn Ala Leu Asn Gly Asn Gly Asp Pro Asn Asn Met Asp Arg Ala 85 90 95 Val Lys Leu Tyr Arg Lys Leu Lys Arg Glu Ile Thr Phe His Gly Ala 100 105 110 Lys Glu Val Ala Leu Ser Tyr Ser Ala Gly Ala Leu Ala Ser Cys Met 115 120 125 Gly Leu Ile Tyr Asn Arg Met Gly Thr Val Thr Thr Glu Val Ala Phe 130 135 140 Gly Leu Val Cys Ala Thr Cys Glu Gln Ile Ala Asp Ser Gln His Arg 145 150 155 160 Ser His Arg Gln Met Val Thr Thr Thr Asn Pro Leu Ile Arg His Glu 165 170 175 Asn Arg Met Val Leu Ala Ser Thr Thr Ala Lys Ala Met Glu Gln Met 180 185 190 Ala Gly Ser Ser Glu Gln Ala Ala Glu Ala Met Glu Val Ala Ser Gln 195 200 205 Ala Arg Gln Met Val Gln Ala Met Arg Thr Ile Gly Thr His Pro Ser 210 215 220 Ser Ser Ala Gly Leu Lys Asp Asp Leu Leu Glu Asn Leu Gln Ala Tyr 225 230 235 240 Gln Lys Arg Met Gly Val Gln Met Gln Arg Phe Lys 245 250 <210> 64 <211> 230 <212> PRT <213> NS of A/swine/Korea/S83/2004(H9N2) <400> 64 Met Asp Ser Asn Thr Val Ser Ser Phe Gln Val Asp Cys Phe Leu Trp 1 5 10 15 His Val Arg Lys Arg Phe Ala Asp Gln Glu Leu Gly Asp Ala Pro Phe 20 25 30 Leu Asp Arg Leu Arg Arg Asp Gln Lys Ser Leu Arg Gly Arg Gly Asn 35 40 45 Thr Leu Gly Leu Asp Ile Glu Thr Ala Thr His Ala Gly Lys Arg Ile 50 55 60 Val Glu Arg Ile Leu Glu Glu Glu Pro Asp Glu Ala Leu Lys Met Ala 65 70 75 80 Val Ala Ser Val Pro Ala Ser His Tyr Leu Ala Asp Met Thr Leu Glu 85 90 95 Glu Met Ser Arg Asp Trp Phe Met Leu Met Pro Lys Gln Lys Val Ala 100 105 110 Gly Ser Leu Cys Ile Lys Met Asp Gln Ala Ile Met Asp Lys Asn Ile 115 120 125 Thr Leu Lys Ala Asn Phe Ser Val Ile Phe Asn Arg Leu Glu Thr Leu 130 135 140 Ile Leu Leu Arg Ala Phe Thr Glu Glu Gly Val Ile Val Gly Glu Ile 145 150 155 160 Ser Pro Leu Pro Ser Leu Pro Gly His Thr Asn Glu Asp Val Lys Asn 165 170 175 Ala Ile Gly Ala Leu Ile Gly Gly Leu Glu Trp Asn Asp Asn Thr Val 180 185 190 Arg Val Ser Glu Thr Leu Gln Arg Leu Thr Trp Arg Ser Ser Asp Glu 195 200 205 Asn Gly Gly Pro Ser Leu Pro Pro Lys Gln Lys Arg Lys Met Ala Arg 210 215 220 Thr Ile Glu Ser Glu Ile 225 230 <210> 65 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer for PB2 gene <400> 65 gattcagctg atagtgagtg 20 <210> 66 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer for PB2 gene <400> 66 agtagaaaca aggtcgtttt 20 <210> 67 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer for PB1 gene <400> 67 caactagaaa gaagattgaa 20 <210> 68 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer for PB1 gene <400> 68 agtagaaaca aggcattttt 20 <210> 69 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer for PA gene <400> 69 aaaggtagac tttgacgatt 20 <210> 70 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer for PA gene <400> 70 agtagaaaca aggtactttt 20 <210> 71 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer for HA gene <400> 71 tattcgtctc agggagcaaa agcagggg 28 <210> 72 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer for HA gene <400> 72 acagagttca ccttgtttgt aatcc 25 <210> 73 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer for NP gene <400> 73 atggagactg atggagaacg 20 <210> 74 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer for NP gene <400> 74 atcaattcca tcaccattgt tcc 23 <210> 75 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer for NA gene <400> 75 atgaatccaa accagaaaat a 21 <210> 76 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer for NA gene <400> 76 cttcctccaa ctttttatgg 20 <210> 77 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer for M gene <400> 77 tattcgtctc agggagcaaa agcaggtag 29 <210> 78 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> primer for M gene <400> 78 atatcgtctc gtattagtag aaacaaggta gttttt 36 <210> 79 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer for NS gene <400> 79 tattcgtctc agggagcaaa agcagggtg 29 <210> 80 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> primer for NS gene <400> 80 atatcgtctc gtattagtag aaacaagggt gtttt 35

Claims (4)

  1. 인플루엔자 바이러스 A/Swine/Korea/S109/2004(H9N2)(KCTC 10761BP).
  2. 인플루엔자 바이러스 A/Swine/Korea/S109/2004(H9N2)(KCTC 10761BP)의 사균 또는 이의 항원을 유효성분으로 포함하는 인플루엔자 바이러스 백신.
  3. 제 2항에 있어서, 상기 항원은 서열번호 44에 기재된 아미노산 서열을 포함하는 HA 단백질 또는 서열번호 46에 기재된 아미노산 서열을 포함하는 NA 단백질인 백신.
  4. 제 2항에 있어서, 상기 백신은 돼지 또는 사람에 적용되는 것인 백신.
KR1020050010416A 2005-02-04 2005-02-04 돼지 인플루엔자 바이러스ａ／ｓｗｉｎｅ／ｋｏｒｅａ／ｓ109／2004(ｈ9ｎ2) 및이의 백신 KR20060089391A (ko)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102093982A (zh) * 2010-02-10 2011-06-15 河南农业大学 一种禽流感h9n2亚型病毒毒株及其应用
CN103497933A (zh) * 2013-07-30 2014-01-08 北京华夏兴洋生物科技有限公司 一株h9n2型禽流感毒株在疫苗开发上的应用
CN107151659A (zh) * 2017-03-01 2017-09-12 苏州系统医学研究所 一种流感病毒株及其应用
CN112574958A (zh) * 2019-09-28 2021-03-30 普莱柯生物工程股份有限公司 一株h9亚型禽流感病毒分离株及其应用

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102093982A (zh) * 2010-02-10 2011-06-15 河南农业大学 一种禽流感h9n2亚型病毒毒株及其应用
CN103497933A (zh) * 2013-07-30 2014-01-08 北京华夏兴洋生物科技有限公司 一株h9n2型禽流感毒株在疫苗开发上的应用
CN103497933B (zh) * 2013-07-30 2016-05-11 北京华夏兴洋生物科技有限公司 一株h9n2型禽流感毒株在疫苗开发上的应用
CN107151659A (zh) * 2017-03-01 2017-09-12 苏州系统医学研究所 一种流感病毒株及其应用
CN112574958A (zh) * 2019-09-28 2021-03-30 普莱柯生物工程股份有限公司 一株h9亚型禽流感病毒分离株及其应用
CN112574958B (zh) * 2019-09-28 2022-09-09 普莱柯生物工程股份有限公司 一株h9亚型禽流感病毒分离株及其应用

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