KR20060033345A - Herbextract for removing harmful elements caused by smoking and manufacturing method of the same - Google Patents

Abstract

The present invention relates to a herbal extract for the removal of nicotine and tar components due to smoking and to strengthen the softening in the human body and a method for producing the same. The method of manufacturing the herbal extracts comprises a first step of drying the aloe, marigold, camellia, samchil, tea tree, buckwheat, bird vine, honeysuckle, licorice and tea vinegar, respectively, to form a dry matter, and a solvent in each dry matter of the first step. A second step of forming an extraction solution in which the dried component is extracted in a solvent by heating the mixture, a third step of removing the solid material by filtering the extraction solution of the second step, and the filtered extraction solution of the third step And a fifth step of obtaining each extract by evaporating the solvent component contained therein and a fifth step of mixing the extract of the fourth step in a predetermined ratio to provide a mixed extract.

The herbal extract according to the present invention is effective in reducing the amount of tar and nicotine in the blood accumulated in the human body due to tobacco smoking. In addition, the herbal extract of the present invention provides an advantage that can be easily added to the functional food to be prepared and supplied in various forms of preparation.

Herbal extracts, nicotine, tar, tobacco.

Description

Herb extract for removing harmful components caused by smoking and its manufacturing method {Herbextract for removing harmful elements caused by smoking and manufacturing method of the same}

The present invention relates to a herbal extract for removing harmful components due to smoking and a method of manufacturing the same. More specifically, the present invention relates to a herbal extract for the removal of nicotine and tar components due to tobacco smoking and a method of manufacturing the same.

Tobacco accounts for an increasing proportion of substances that harm human health. The research on the relationship between smoking and disease occurrence has been intensively studied since the mid 1900s, and there is a continuous evidence of increasing the incidence of various diseases including lung cancer caused by smoking and other health disorders. In fact, tobacco smoke consists of mainstream smoke and sidestream smoke, which is smoke after smoker breathes, and smoke refers to live tobacco smoke from the end of a burning cigarette.

As a result of the component analysis of tobacco smoke, about 4,000 species were identified. About 4,000 species account for more than 95% of the total weight of the mainstream smoke, while thousands of other components are present in extremely small amounts. Tobacco smoke is caused by incomplete combustion of tobacco, and when it is completely burned, water and carbon dioxide are generated. Incomplete combustion occurs through the incomplete combustibility of some tobacco leaf components, insufficient oxygen supply, as well as distillation, sublimation, pyrolysis, thermal synthesis, and volatility during tobacco burning.

Tobacco smoke is an aerosol that is a mixture of liquid or fine particles in a gas.A Cambridge glass fiber filter is a filter that can filter 99% or more of particles larger than 0.1 µm in diameter. Substances passed through) are called gas or vapor components. The gas or vapor component is made up of nitrogen (59%), oxygen (13.4%), carbon dioxide (13.5%), carbon monoxide (3.2%), and the like, aldehydes, ketones, alcohols, esters, etc., which are toxic to bronchial cilia. Volatile components having the same low molecular weight are included.

The material filtered by the camber glass fiber filter is also called Particulates phase components, which is about 8% of tobacco smoke, and there are at least 38 known carcinogens, including carcinogens, and carcinogens and nicotine, Other inorganic and organic chemicals are included.

Recent research suggests that in the 21st century, tobacco is still a major risk factor for human health, but nevertheless, it is not easy for tobacco lovers to quit smoking habits. There is a problem that nicotine and tar components accumulate in the body due to tobacco smoke.

 The present invention has been made to solve the above problems, and an object of the present invention is to provide a herbal extract that can remove the accumulated nicotine and tar components of the human body due to smoking and a method of manufacturing the same.

Still another object of the present invention is to provide a herbal extract and a method for preparing the same, which can provide a functional food product by extracting a herbal extract that can remove harmful components caused by smoking.

Herbal extracts for removing harmful components due to smoking according to the present invention for achieving the above object is 0.5 to 10% by weight, marigold 5 to 40% by weight, 5 to 20% by weight in black, 0.5 to 10% by weight, green tea 5 to 20% by weight of leaves, 5 to 40% by weight of chajeoncho, 5 to 40% by weight of weak buckwheat, 5 to 20% by weight of bird vines, 5 to 30% by weight of honeysuckle and 0.5 to 10% by weight of licorice.

According to the present invention, a method of preparing a herbal extract for removing harmful ingredients due to smoking according to the present invention comprises drying aloe, marigold, black yam, samchil, green tea leaf, weak wheat, bird vine, honeysuckle, licorice and tea vinegar, respectively. A first step of forming a dry matter, a second step of forming an extraction solution in which the components of the dry matter are extracted in a solvent by adding a solvent to each of the dry matters of the first step, and filtering the extracting solution of the second step The third step of removing the solid material and the fourth step of obtaining each extract by evaporating the solvent component contained in the filtered extract solution of the third step and the extract of the fourth step is mixed and mixed in a predetermined ratio A fifth step of providing an extract.

According to another aspect of the present invention, the manufacturing method of the herbal extract for removing harmful ingredients due to smoking is dried aloe, marigold, black yam, Samchil, green tea leaf, weak wheat, bird vine, honeysuckle, licorice and tea vinegar to form a dried product, respectively. The first step, and the second step of mixing the dry matter of the first step in a predetermined ratio to provide a mixed dry matter, the water-soluble solvent is added to the mixed dry matter of the second step and heated to extract the components of the dry matter to the solvent A third step of forming a mixed extract solution, a fourth step of filtering the mixed extract solution of the third step to remove solids, and a solvent component contained in the mixed extract solution filtered in the fourth step And a fifth step of providing a mixed extract.

Hereinafter will be described in detail a preferred embodiment of the herbal extract for removing harmful components due to smoking and a method for producing the same according to the present invention.

These examples are intended to illustrate the invention and the scope of the present invention is not limited only to these examples.

Examples of herbal extracts for eliminating harmful components due to smoking according to the present invention include aloe 0.5 to 10% by weight, marigold 5 to 40% by weight, 5 to 20% by weight, 5 to 20% by weight, 0.5 to 10% by weight, green tea leaves 5 to 20 It includes 5% by weight, 5-40% by weight of chajeoncho, 5-40% by weight of weak buckwheat, 5-20% by weight of vines, 5-30% by weight of honeysuckle and 0.5-10% by weight of licorice.

The ingredients used in the herbal extracts according to the present embodiment and the effects thereof are as follows. For convenience of explanation, each material is assigned a serial number (1) weak wheatgrass, (2) bird vines, (3) honeysuckle, (4) Licorice, (5) Marigolds, (6) Acorns, (7) Aloe, (8) Samchil, (9) Green tea leaves, and (10) Charcoal candles are described in detail below.

Houttuynia cordata Thunb (Polypara cordata Bueck) of ingredient (1) is also called medicinal herb, succulent, three hundred vine, effervescent and ten, and the stem and leaves of the plant are used.

The main components are methylnonylkenone (CH ₃ (CH ₂ ) ₈ COCH ₃ ; oxidation product of decanoyl acetaldehyde) myrcene (C ₁₀ H ₁₆ ), laurin aldehyde (C ₁₁ H ₂₃ CHO), caprinaldehyde (C ₉ H ₁₉ CHO), carplic acid, etc., and leaves contain 2.7% of quercitrin and minerals released in water.

Cuercitrin has urinary action (100,000-fold concentration), cardiac action, antibacterial action against Escherichia coli, typhoid bacteria, paratyphoid bacteria, erythropoies, gonococci, staphylococci and filamentous fungi, and capillary potentiation.

Decanoyl acetaldehyde has antimicrobial activity against non-pathogenic fungi, as well as ringworm and athlete's foot fungi, as well as fungi against staphylococcus aureus, gonococcus and anti-acid bacteria. Antimicrobial activity against staphylococcus is 1: 40,000 and is stronger than sulfa.

Outpost is used as an inflammatory and diuretic detoxification agent, and it is used for gonorrhea, urethritis, cystitis, uterus, pneumonia, bronchitis, bite, athlete's foot, anti-depressive, swelling, windworm and sinusitis. Prepared with juice as a folk prescription, attach when athlete's foot, hemorrhoids, snake venom and lacquer rise. The leaves and stems are urinary medicines for swelling and each other. The roots are used for wind poison and swelling.

Cuscuta japonica Choisy of ingredient (2) is picked from the seeds and dried in the sun. Seeds contain about 40mg% of resin-like glycosides, amylase and provitamin A, sugars, and camphorol in the outpost.

It is used as a gangjeong tonic for the treatment of gastrointestinal disorders, oil wells, and back pain. It is also used as a sedative, pain medicine, and diarrhea. Folk prescription is to wash the face to remove papules with scallop juice, sputum pill, blood pill, sweat pill, old wind pill, fever pill, sputum pill, insect pest, diarrhea, pneumonia, kidney disease and high blood pressure. Used for the treatment of

Lonicera japonica Thunb of ingredient (3) is also called gold, silver and mistletoe, and uses the stem and leaves of the plant.

Flowers include luteolin (C ₁₅ H ₁₀ O ₆ ; 5,7,3,4-tetrahydroxyflavones) and inositol (C ₆ H ₁₂ O ₆ ). Flowers are due to inositol and lead-like substances (seryl) Alcohols, sterols, arachnic acid, myristic acid, ellidinic acid, linoleic acid, linoleic acid).

The leaves and stems contain 5-8% tannins, and the leaves contain loganine, about 19% nitrogen-containing substances, and roniserine (C ₂₇ H ₃₀ O ₁₅ 2H ₂ O), a luteolin-7-rhamnoglucoside. have. Irinoid compounds in the leaves of the same genus plant (L. morronii) are roniseiroid, sweroside.

Flower decoction has the effect of urinating and increasing blood sugar and preventing the action of pneumoniae, typhoid, staphylococcus, and pneumococcal pneumoniae, peptic ulcer, sympathetic nerve excitement and smooth muscle paralysis.

In motion therapy, flowers, leaves, and stems are used for fever, poison, urine and sperm medicine in the early stages of wind music. Burnt like char is used as a medicine for blood.

It is used for the treatment of cancer as a private prescription. Drinking water for a long time like tea is effective for gastric cancer, endometritis, fistula of lymph node tuberculosis and ophthalmitis.

The licorice (Glycyrrhiza glabra L.) of the ingredient (4) is called a national dish, straight licorice, a sensitive herb and uses the root of licorice. In the fall, root stems and roots are carved out, trimmed, washed in water and dried in the sun. Root stem and root glycyrrhigen content is higher than summer, and biennial licorice content is 3.8% in April, 3.7% in May, 3.3% in July, 3.8% in August, 5% in September, 6% in October. According to the parts of the roots, the content of glycyrrhizin is 6.91% in the root of the biennial licorice and 8.04% in the thin root. Therefore, it is preferable to use small roots in the root and root stem. Also, the skin (13.06%) is higher than the neck and water (10.42%). When peeling, only the corked skin should be peeled off, so that the skin is not damaged. Roots and stems contain 5-14% glycyrrhizin (potassium or calcium salt of glycyrrhizin), many 23%, and glabraic acid (glyciretin; C ₃₀ H ₄₆ O ₅ ).

Glycilisic acid C ₄₂ H ₆₂ O ₁₆ is triterpenoid saponin, melting point 205 ℃, decomposition point 220 ℃, [d] _D ²⁰ + 58.5 ℃, well soluble in alcohol and hot water and not soluble in ether. After cooling in hot water, it hardens like grass. Decomposition of water results in tasteless glycyrrhetinic acid (C ₃₀ H ₄₆ O ₄ ) and two molecules of glucuronic acid (C ₆ H ₁₀ O ₇ ). Glycyretinic acid is a β-amirin-based triterpenoid, which has two kinds of α and β substances, which are easily changed. But its esters do not change from one another. α-glyciretinic acid is a dilute alcohol of plate crystals, melting point 283 ° C, [d] _D ²⁰ + 140 ° C (ethanol) and solved with ethanol, dioxane and chloroform. β-Glycyretinic acid is needle crystal (ethanol), melting point 296 ~ 300 ℃, [d] _D ²⁰ + 86 ℃ (alcohol), soluble in ethanol, pyridine, acetic acid and not soluble in petroleum ether.

In addition, flavono glycosides, liqueurin C ₂₁ H ₂₂ O ₉ , neoriquiritin, and lamnoricuritin (these non-sugar parts are all liqueurininin) and the corresponding galcon glycosides, such as isoricuitin and neo Isoricuiritin, isoram norikuiritin, and liqueur seed (the non-sugar part of these are all Isorikuuritingenin). Rhamnoricuritin is also called liqueuritoside.

Chromatographs from licorice root identified more than 27 flavonoid analogs. Β-sitosterol, formmononetin, ricolycidine, lycoricone, and ricore-oligonane were identified in the roots of curved licorice. In addition, 14 to 22% protein, 2 to 5% oil, 36 to 62% nitrogen-free extract, and 5 to 20% ash are used for feeding beasts on dry leaves and stems when flowering.

Flavonoid content in licorice changes with the season of growth. The roots are high in autumn and the leaves and stems are highest when the berries begin to run. The content of liqueur seeds in autumn is about 0.5%. Licuraside accounts for about 10% of the total flavonoids.

There are saponaretin, glyphoside and bitexin on the stem and leaves. Saponaretin is about 50% of the total flavonoids and 3% in the outpost when fruit is opened.

Glyceramarin (bitter component of glycyrrhizine), 2,4,4-trihydroxygalcon and its glycosides Dioxystigmasterol (melting point 135 ~ 136 ℃), β-sitosterol, essential oil (0.03%), Ascorbic Acid 11-30mg%, Asparagine (1-4%), Fructose (2.4-6.5%), Glucose (2-4%) Mannitol, Starch (14-30%), Gum (1.5-4%), Yellow There are amino acids including bovine, malic acid, oil, resin, tannin, protein and aspartic acid, proline, alanine, threonine, serine, glycine, valine, isoleucine, and γ-aminobutyric acid. The strongest bitter taste is 8%. Among them, 2.5 ~ 3% of the water is released.

Glycirizine is known as an active ingredient of licorice root and has a sweet taste of about 40-50 times that of candy, and its sweetness can be felt even in a dilution of 100,000 times. The water solution foams when shaken, but is weaker than normal saponins and has no or hemolytic action (potassium salt is 1: 400). The glycyrrhetinic acid produced by the hydrolysis of glycyrrhizin is not sweet and hemolytic. Thus, the expectorant action of licorice is presumed to be due to glycyrrhetinic acid. Glysirizine also relieves the respiratory system.

Licorice root extract and glycyrrhizin are non-toxic and relieve poisoning by catcherchloral, strycnin, yohimbine, cocaine, morphine, codeine, atropine and luminal. Releases bacterial and viral toxins such as tetanus toxin, diphtheria toxin and snake venom.

It antagonizes with acetylcholine, histamine, and allergens, and has an adrenaline-like effect.

The poisonous action of glycyrrhizin is related to glucuronic acid, a product of water degradation. Glucuronic acid is a component widely found in animal and plant systems and is combined with toxic substances in the liver to form glucuronide and excreted by urine. Therefore, glucuronic acid is used for drug addiction, alcoholism, food poisoning, jaundice, cirrhosis of the liver, chronic liver, rheumatism, joint pain, nerve pain. The detoxification of glycyrrhizin is related to the action of glucuronic acid as well as the corticosteroids of glycyrrhizin. In other words, glycyrrhizin has an effect similar to that of the adrenal cortex hormone desoxycorticosterone.

Glycyretinic acid reduces the excretion of sodium, chlorine and urine and increases the excretion of potassium in animal experiments. That is, the ratio of sodium and potassium (Na / K) is lowered. As such, it negatively affects potassium metabolism but positively affects sodium metabolism. It also acts to reduce the ascorbic acid content of the adrenal cortex to enhance the effects of corticosteroids on water and salt metabolism. This action is found in licorice extract and glycyrrhizin. The same thing happens when a person eats.

Licorice root extract glycyrrhizin is anti-inflammatory. It has a positive effect on the treatment of formalin arthritis caused experimentally in animals. It inhibits the activity of serum transaminase, increases the activity of adenosine triphosphatase in the liver, and anti-inflammatory action similar to the desoxycorticosterone of glycyrrhizine is due to its degradation product glycyrrhetinic acid.

There is also a view that the corticosteroid-like action of glycyrizine inhibits the reduction of the inactivating Δ ⁴ -3 ketone moiety in vivo.

Licorice root extract has an anti-muscular effect similar to papaverine on gastrointestinal smooth muscle in animal experiments. This action is due to the flavonoid component. Licorice flavonoids, which have not been used until now, was developed with known antifungal and anti-inflammatory effects. Licuraside, a flavonoid component, has three times as hard as papaverine and has anti-inflammatory and anti-ulcer activity. And antagonists of acetylcholine and histamine.

Licorice root extract has been known to have a therapeutic effect on peptic gastric ulcers in the 1950s, and many experiments have been conducted to find anti-ulcer substances in licorice roots. In the 1960's, flavonoids were obtained and flavonoids were found to be active. In addition, recent experiments have shown that there are anti-inflammatory and anti-ulcerative effects as well as palliative action.

Licorice has a strong surface activity, so if you eat it with other copper medicines, it helps loosen the ingredients in water to help absorption. It also reduces the toxicity of the medicinal herbs and changes the taste. Licorice root has an inhibitory effect on staphylococcus aureus, Escherichia coli, and bacilli. When glycyrrhizin is used with anti-cancer drug thiopoxamide, anti-cancer activity is 85-95%. Licorice root's actions include sputum cutting, alleviating, anti-inflammatory and jingyeong, and antihistamine.

Therefore, it is used for upper respiratory tract disease as sputum aggression. Because of its sweetness, it is also used as a drug-inhibiting agent. It can also be used as a poison or medicine. Nowadays, it is widely used for gastroduodenal ulcers, Edison disease, bronchial asthma, jaundice and hepatitis, eczema and other skin diseases. Glycilisic acid is used as a supplement to increase the activity of anticancer drugs.

In ligament therapy, licorice root is used to alleviate and harmonize the effects of other medicines. In other words, when used with a hot medicine to relieve heat, and when used with a cold to relieve the cold. Like this palliative medicine or pain 멎 jinyongjeok, sputum medicine, cough 멎 yigeok, poison grass medicine is used for stomach cramps, stomach pain, sore throat, stomach ulcer and duodenal ulcer and used in the prescription of the poisonous consent.

Calendula officinalis L. (5), also called calendula, uses the petals of marigold and dried in the shade. The main ingredients are carotene, lycopene (melting point 175 ℃), violaxanthin (C ₄₀ H ₅₆ O ₄ ; melting point 207 ~ 208 ℃), rubyxanthin (melting point 160 ℃), neoricopin A, citraxanthin ( Flavonoids such as melting point 163 ~ 164 ° C) and clavochrome (melting point 184 ~ 185 ° C). The content of carotenoids is about 3%.

Flowers contain 0.02% essential oils, about 3.4% resins (about 1.5% nitrogen content), albumin 0.64%, organic acids calculated from malic acid 6.84%, small amounts of salicylic acid and alkaloids. Above ground, calenden (C ₂₃ H ₃₈ O ₇ ), saponin (when decomposes into oleanolic acid and glucuronic acid), arnidiol, paradiol, tannin (6-7%), unsaturated triterpenediol There is this. Seeds include alkaloids, oils (primarily glycerides of lauric acid and palmitic acid), and seryl alcohol and phytosterol in the unsaponified portion of the oil.

As a function, flower decoction, alcohol extract, and juice have a calming effect on the central nervous system in animal experiments and lower reflex excitability. It also lowers blood pressure, hardens heart contractions, slows heart rhythms and sterilizes.

Make a plaster and apply it to the burns, ulcers, dentitions, crows, and crows. Also, rainforest medicine and tinctures are affected by ophthalmitis, alveolar pylori and uterine fistula, cervical erosion, and trichomoniasis vaginitis (2% tincture). Rainforest medicine and tincture are used for gastric duodenal ulcers, hepatitis, streptococcitis, and saccharitis as inflammatory drugs and fever-fighting drugs. It is also used for high blood pressure and heart disease. The combination of pollen and nicotinic acid is used as a cure for inoperable stomach cancer. If you use such drugs, symptoms of intoxication, indigestion, burping, nausea are reduced, the taste of rice is good, and sleep is good.

The solanum nigrum L. of the ingredient (6) is also called the crow, larvae, and crow. The fruit of the crow is round and ripe in black. When blossoming outposts, cut the stems and dry them in the shade.

The main ingredients in the outpost are steroid alkaloids, small amounts of atropine and nicotine. Steroidal alkaloids include solanine (C ₄₅ H ₇₃ O ₁₅ N; solanidine-β-l-rhamnoside-d-galactosid-d-glucoside), solasodine, solanigrin and the like. Outposts also include flavonoids, rutin, asparagine, solangustin, cytosterol 7-10% tannins, saponins (diosgenin, tigogenin), and organic acids. Coconut has similar ingredients to the outpost. Ripe fruits include glycoalkaloids, saponins and anthocyanin pigments, β-carotene (318-503 mg% as carotene), 1,630 mg% ascorbic acid, and also solonanin (0.2%) and solamargin (0.25%). The leaves contain between 24 and 184 mg of ascorbic acid and 50 mg of carotene. The roots also contain alkaloids and saponins.

As a function of the outpost decoction is typhoid bacteria, staphylococcus aureus, Pseudomonas aeruginosa, E. coli, E. coli, fungi, anti-inflammatory, blood sugar lowering action. Alkaloids promote histamine degradation in lung and bronchial tissues, so coughing, sputum sputum and blood pressure lowering. Very small amounts of atropine make the pupils larger, which may result in better vision, even temporarily, in patients with severe Batu Boi who suffer from mild paralysis of eye control.

In motion therapy, it is used to relieve fatigue with tonic, fever and urine medicines. Leaves and stems are attached to various wounds and cuts, and fruit brightens the eyes. In civilian use, it is used as a tonic and urinary medicine for sinseokjung and bite. Starch juice is used for colds as a sedative, cough and sweating medicine. Flowers are sputum, leaves are astringents, blood drops and diarrhea. Leaf and Fruit Weinum is an antiseptic and inflammatory drug used for hemorrhoids, ulcers, wounds, swelling, ringworm, eczema, navel, soles, and strokes. It is also used for head pain, rheumatism, gout and tumor.

About 170 species of Aloe spp. Are known as Aloe sp of component (7). Many leaves gather on short stems. The leaves are live with thorns on the edges. White, yellow and red flowers gather together like Isaac. Growing in the tropics reaches 4m high and leaves 65cm long. Aloe plants differ in origin depending on the region. In Korea, long-leaved aloe A. arborescens, speckled leaf aloe A. saponaria, broad-leaf aloe A. arborescens var natalensis. Aloe (leaf juice) contains aloe phosphate (C ₂₀ H ₁₈ O ₉ , aloe emodine-d-arabinoside) 16-20%, aloe-emodin natalo, labarberon (isoemodine) And 12% of resin (cinnamic acid ester of allolecinotanol C ₂₂ H ₂₆ O ₆ ) is contained.

Aloe (dried leaf juice) is bitter tonic, digestive and appetite suppressant at 0.02 ~ 0.05g at one time, weak diarrhea at 0.02 ~ 0.2g at once, and strong diarrhea at 0.3 ~ 0.5g at once. In motion therapy, 1-2g is used for children's markers and constipation.

Aloe juice: Fresh juice containing 20% ethanol for squeezing juice is used as a fungicide to clean wounds or rotted areas of inflammation. Aloe leaf: used to make biostimulants. Slice the raw leaves and leave for 12 days in adverse conditions, ie dark and low temperatures. At this time, the leaves are said to produce substances that stimulate the vital activity weakened due to adverse living conditions. This material was termed a biostimulator, and its chemical composition is still unknown. It is generally regarded as an aliphatic dicarboxylic acid and an aromatic organic acid having a large molecular weight. The treated leaves are crushed with a grinder, placed in a volume of 3 times water at room temperature, filtered and sterilized to prepare injections. Or lactose and oil are also prepared. Injectables are used to enhance the patient's defenses, while lactose is used to prevent skin damage during radiation damage. Aloe juice: As a bio-irritant, mix 80 ml of fresh aloe juice with 20 ml of 95% ethyl alcohol and 0.5% chlorbutanol hydrat.

For gastritis, gastroenteritis, colitis, constipation, take a small spoonful or half a tablespoon two to three times a day for 30 minutes, 15 to 30 days before eating. It can also be applied to the treatment of wounds, burns, skin and mucositis. Aloe X extract is used for gastroduodenal ulcers, chronic pneumonia, bronchial asthma, eye diseases and other diseases.

Panax notoginseng (Burk) F.H.Chen of ingredient (8) is called Geumhwanhwan, Ginsengsamchi, Samsamchil, Jeonsamchil, Sanchil, and 3 ~ 8% of saponin at the root of Samchil.

The main components are ginsenosides Rb ₁ , Rg ₁ , Rg ₁ , and there are small amounts of ginsenosides R _a , R _b2 , R _d , R _e , R _c , and R _o is very little. The composition of essential oils is less than ginseng.

Root is bloody, strong heart. In animal experiments, it increases blood flow in the coronary arteries, reduces oxygen consumption in the myocardium, and reduces the amount of lipids and cholesterol in the blood. The root is used for angina myocardial infarction hypertension hypercholesterolemia. It is also used for chronic and acute hepatitis. In China, roots are used as drugs to lower the pain, pain, and inflammation. In particular, the blood effect is obvious, it is used in cuts, nosebleeds and various kinds of women's blood. This plant was used as a bloody medicine in folklore. Samchil is called three-branch leaves with seven leaves, and its shape is similar to Chosun Ginseng. The roots are trimmed and the roots are trimmed and dried ginseng and beeswax with rhythm on the side.

Thea sinensis L of component (9) is a evergreen evergreen tree, leaves are thick and long oval, white flowers bloom in autumn, and fruits are flat round shape and have three corners. Jeolla-do, there are some that grow in Gyeongsang-do, but they are often planted. In addition, the processing method is largely divided into green tea, black tea, flower tea (hwacha).

Green tea is picked and steamed in medium or inactivated by oxidizing enzymes with hot air and then dried. When the shoots sprout in spring, they block the sunlight to prevent tannins, and after picking the cultivated shoots, they are steamed in the middle and dried. Black tea is picked and dried in half, then crushed and stacked overnight. At this time, the tannin is oxidized by the action of the enzyme to have a red color. This is dried by hot wind. Flower tea is a mixture of leaves and flowers, piled up, absorbed with floral fragrance, and dried.

The main components of the leaves are 1-6% of purine bases such as caffeine (C ₈ H ₁₀ O ₂ N ₄ ), xanthine (C ₅ H ₄ O ₂ N ₄ ) and theophylline. It also has 9-15% tea tree tannins. Among them, soluble tannin is about 3% and insoluble tannin is 9.9%. Tea tree tannin (C ₂₂ H ₁₈ O ₁₀ ) is a molar ester of theacatechol, 3-galloyl-l-epi-gallocatechol. In addition, steroid thetasterol (C ₂₉ H ₅₀ O), a-spinasterol and triterpenoids β-amirin (C ₃₀ H ₄₈ O) Palthreubin (C ₃₀ H ₅₀ O) and carotene vitamin C, Quercetin, camphorol and the amino acids arginine and theanine. The sweetness of tea is mainly due to theanine. Green tea contains about 0.006% of essential oils, the main components of which are α-β-hexenol (CH _3- CH _2- CH = CH _- CH _2- CH ₂ OH) (50-60%), α-β-hexenol (CH _3- (CH ₂ ) ₂ -CH = CH _- CH ₂ O) (5%), isobutylaldehyde, isovalerylaldehyde, methylethylacechoaldehyde, gilacetic acid, methyl salicylate, benzyl alcohol, linalool Etc. Alkaloids include theophylline, theobromine, xanthine, adenine, hypoxanthine, paraxanthine, methylxanthine and isatin. It also contains lecithin, nucleotide adenine, nucleotide cytosine, nucleorotate and coumarin (umbeliferone-7-glucoside) containing iron and manganese, vitamins B ₁ , B ₂ , B ₃ , B ₅ , K ( 156 ~ 233mg%). And essential oils, flavonol glycosides (quercetin and camphorol derivatives). Seeds contain 22% caffeine oil (18% in the seed) theasaponin (C ₅₇ H ₉₀ O ₂₆ ).

Theasaponin is hydrolyzed to theaasapogenol (C ₃₀ H ₅₀ O ₆ ), glucuronic acid (C ₆ H ₁₀ O ₇ ), galactose, arabinose, xylose, and angelic acid. Seeds contain 33% starch and 8.5% protein. Stems, roots, and seeds contain steroid saponins, especially in seeds (9-10%).

Tea trees have been used to excite the nervous system and strengthen mental and physical activity. Feeding children with low levels of prothrombin in their blood increases their content.

Tea Leaves: Used as a source of caffeine and tea tree cadkin. These drugs are used for hemorrhagic predisposition, retinal bleeding, photopathy, and high blood pressure when the weakness of the blood vessels and the permeability are destroyed. Caffeine is used for tension-induced weakness in general, low blood pressure, acute infectious diseases, and mental and physical fatigue when heart activity is weak. It is also used as a poison medicine for alcoholism and opioid poisoning. Theophylline improves the blood circulation of the heart and is used as a pee medicine for blood circulation disorders that result from dysfunction of the heart and kidneys. Theobromine is used in the formulation of drugs called diuretin and theophylline, which are used for cardiovascular disorders, hypertensive cardiac edema, and opiphyline, which are used for bronchial asthma and angina.

Green tea leaves are immediately dried without picking young leaves. The fragrance is weak but physiologically active. Since green tea has antimicrobial activity, it is used as a decoction for proper treatment. In other words, put 2L of water in front of 100g dry anchovy, soak for 20-30 minutes, boil for an hour, filter it with a double layer of cloth, and take 1 to 2 spoons 3 times a day 20 ~ 30 minutes before eating. 5-10 days for Venus Ely, 16-20 days for Chronic. It is also taken for colitis, proctitis and dyspepsia. To prevent cold and sunburn, apply the green tea with water. It also relieves soreness and heat from sunburn.

There are many ways to drink tea. You can drink dark tea with milk and drink it with hot water or candy. Drinking tea in the morning can have a positive effect on your daily life because it contains many different ingredients necessary for human activities.

Plantago asiatica L. (P.major L. var.asiatica), under the different name, has a long janggu and a baejanggi, and cuts the bottom of the leaf when it blooms and dries it in the sun. Audine glycosides (C ₁₅ H ₂₄ O ₉ ), catalpol and flavonoids flantaginine (scutellalane-7-glucoside), homoplantaginine (hispitolin-7 glucoside). Lantagin (5,6,4-trimethylscutellane-7-glucoside), in addition to apigenin, luteolin, nefetin, and 6-oxyluteolin glycosides, leaves also include ursolic acid and hentri Palmitic acid esters of acontane (C ₂₃ H ₄₈ ), β-sitosterol, stigmasterol, and β-sitosterol, as well as enzymes (emulsine and inverotene), carotene and ascorbic acid. Partial hydrolysis of mucus results in the disaccharide I [O-β-D-xyclopyranosyl- (1 → 2) -D-xyclopyranose. ], II [O-β-D- xyclopyranosyl- (1 → 4) -D- xylopyranose], III [(O-β-D-glucopyranosyl-uronic acid- (1 → 5) ) -L-arabinopyranose], as well as plantenolic acid (plantagoic acid), succinic acid, choline, adenine, vitamins A and B _1. Outpost contains polysaccharides that are released in water. Up to 28%, and 50% can be removed by electrodialysis, and long-term purification yields 0.4-0.5% ash, polysaccharides 80-82% pectinic acid, 5-6% galactoaraban 4 Consists of ~ 5% galactan.

When hydrolyzed, pectinic acid produces 78-83% of galacturonic acid, galactose, arabinose, rhamnose, small amounts of glucose, xylose and three other sugars. That is, the polysaccharide is composed of all nine sugars and minerals. Mineral-free polysaccharides contain 66-68% anhydrous galacturonic acid. Hydrolysis of this leads to galacturonic acid. The relative content of polysaccharides of rhamnose, arabinose, galactose, xylose, glucose and mannose is 6: 6: 3.5: 2.5: 1: 1. Galacturonic acid is mainly pectin. In comparison with P.major, which grows plantain in Europe from the standpoint of chemical classification, there is one more irinoid glycoside and 6-oxyluteolin glycoside.

The content of polysaccharides varies between 8 and 19% depending on the growing season. It is the highest when the leaves start to grow in spring and the flower beds begin to emerge. When the fruit is ripe and the flower stalk dries, its content is lowest. Considering the productivity of raw materials, it is best to collect them in June, from the time the buds sprout to the flowers.

The action of outposts and planttagins acts on the respiratory center in animal experiments, slowing and deepening breathing. It also increases the secretion of mucous membranes, increasing the secretion of bronchial mucosa and digestive juices. Seed mucus acts as a relief and fever. The antitussive expectoration of plantain is different from saponin with hemolytic action and irritation to mucous membranes. It is used as a child's cough medicine because it has no hemolysis and no side effects. Plantain also has a peeing action. In addition to increasing water excretion, it also promotes excretion of urea, sodium chloride and uric acid. The polysaccharide component is anti-inflammatory, promotes epithelialization. Polysaccharides isolated from outposts are less toxic. Polysaccharides in rats 1 to 3g / kg, dogs to 2 ~ 5g / kg for 14 days, there is no poison. Polysaccharides inhibit gastric ulcer formation in rats by butadione. Feeding dogs also increases the secretion of gastric juice by 2.5 times and increases the free acidity and total acidity of gastric juice by 20-30%. However, it does not affect the protein lytic activity of gastric juice. Polysaccharides slow the movement of matter three to four times through the stomach. The hardening effect of polysaccharides on isolated intestines of rats is also shown in 1: 10,000 solution.

In addition, polysaccharides have antifungal, anti-inflammatory and epithelialization effects, making them a good treatment for ulcers. Splitting minerals in polysaccharides does not have this effect. Therefore, it is considered that the polysaccharide combined with the inorganic material is a biologically active component. Polysaccharides have been shown to be therapeutic in clinical trials. Gastric duodenal ulcer patients with 0.5 ~ 1g at a time three times a day, 5 to 7 days after the pain disappears, 15 to 20 days after the ulcer symptoms disappear, the acidity of the gastric juice is normal. The functional feature of polysaccharides is to lower the acidity and increase the acidity to normalize the secretion of gastric juice. In particular, patients with normal or low acidity of gastric juice have good therapeutic effect.

Applications include polysaccharides or juices derived from outposts used for duodenal ulcers and chronic gastritis with or without acidity in gastric juice. Outpost decoction is also effective for nephropathy. In copper therapy, outposts are used for gastrointestinal and other arteriosclerosis and diabetes. Mr. Moon drinks from eye diseases and nervous system diseases.

Method for producing a herbal extract for removing harmful components due to smoking according to the present invention will be described in each step in more detail below in the first embodiment.

The first step is a step of drying the aloe, marigold, camellia, samchil, green tea leaf, weak buckwheat, bird vine, honeysuckle, licorice and chajeoncho, respectively, to form a dry matter, the drying of the materials listed above is carried out in the shade without sunlight do. When dried in the sun, due to the destruction or evaporation of the active ingredients contained, the drug is reduced or the amount of extract is reduced. Drying is carried out until the moisture on the surface of the material is almost evaporated. For example, it is preferable to dry the blade until the tip is slightly dried. Since the amount of the solvent added to the dried material varies depending on the weight of the dried material and the drying amount is appropriate, the amount of the solvent can be reduced, so it is preferable to appropriately perform the drying step of each dried product.

In the second step, a solvent is added to each of the dried products of the first step, and the components of the dried products are extracted into the solvent to form an extraction solution. The amount of solvent added to each dried material is mixed about 1.5 to 2.5 L of solvent per 100 g of dry matter.

The solvent of the second step is at least one selected from the group consisting of water, lower alcohols, esters, ketones, aliphatic hydrocarbons and chloroform, preferably water, ethanol, methanol, isopropyl alcohol, n-butanol It consists of at least one selected from lower alcohols, esters containing ethyl acetate, ketones containing cyclo-butanol, cyclo-pentane, n-hexane, chloroform and acetone. The greater the polarity of the solvent of the second step, the more extraction solution can be obtained. In addition, there is a difference in the amount of the extraction solution according to the production site or the collection time of each dry matter of the first step.

In the method of heating each dried material selected from the above-listed solvents, heating is performed for about 1-5 hours under atmospheric pressure using a mantle provided with a reflux condenser. By lowering the atmospheric pressure below atmospheric pressure, the heating time can be shortened. Therefore, if necessary, the air pressure can be lowered to 0.1 atm or less, which greatly shortens the heating time. What is necessary is just to adjust heating temperature suitably in consideration of the boiling point of the solvent used. In general, heating at atmospheric pressure for 1-5 hours dissolves the extract components from the dry matter and may reduce or extend the heating time as necessary.

The third step is to remove the solid material by filtering the extraction solution of the second step. The extraction solution passed through the second step is present in a liquid state in which the dry matter and the solvent are mixed, and the filtered dry solution of the dried state in the extract solution is filtered by a filter to obtain a filtered extract solution in which the dry matter is dissolved.

The fourth step is to obtain each extract by evaporating the solvent component contained in the filtered extraction solution of the third step is a step of heating to a temperature of 40 to 60 ℃ at 0.005 to 0.1 atm. If the evaporation temperature is increased to 60 ° C or higher, there may be a chemical change in the extraction solution or the components of the extraction solution may come out when depressurizing, and when the temperature is 40 ° C or lower, excessive time is required for evaporation. do.

The fifth step is to provide a mixed extract by mixing the extract separated from the extraction solution of the fourth step in a predetermined ratio.

That is, each extract obtained from the fourth step is 0.5 to 10% by weight of aloe, 5 to 40% by weight of marigold, 5 to 20% by weight of yam, samil 0.5 to 10% by weight, green tea leaf 5 to 20% by weight, chajeoncho 5 It is preferable to mix at a mixing ratio of -40 wt%, weak hair 5-40 wt%, fresh ginseng 5-20 wt%, licorice 0.5-10 wt%.

Next, the mixed extract produced in the fifth step is prepared as a functional food by containing 5 to 70% by weight of the mixed extract in a conventional food as an additive object.

When the amount of the mixed extract contained in the food is less than 5%, the removal effect of the harmful component is insufficient, and when the amount of the mixed extract is added 5% or more, the removal effect of the harmful component is remarkable. It is preferable to make it contain%.

In addition, as a functional food to which the mixed extract can be added, there are confectionery such as yogurt, candy, cookies, jelly, chewing gum, soft drinks, etc., and can also be applied in the form of tobacco additives. Moreover, it can also add as a raw material mix | blended with medicine, herbal medicine, etc.

In addition, the mixed extract prepared in the fifth step may be formulated in the form of powder, granules, tablets, capsules and the like by mixing with excipients lactose, corn starch, cellulose, magnesium stearate having a fine crystalline structure.

The second embodiment of the manufacturing method of the herbal extract for the removal of harmful components due to smoking of the present invention is dried aloe, marigold, black yam, samchil, green tea leaf, weak wheat, bird vine, honeysuckle, licorice and tea vinegar respectively to form a dried product The first step, and the second step of mixing the dry matter of the first step in a predetermined ratio to provide a mixed dry matter, the water-soluble solvent is added to the mixed dry matter of the second step and heated to extract the components of the dry matter to the solvent A third step of forming a mixed extract solution, a fourth step of filtering the mixed extract solution of the third step to remove solids, and a solvent component contained in the mixed extract solution filtered in the fourth step The fifth step is to provide a mixed extract.

This embodiment is the same as in the first embodiment except that the dried solution of each material is pre-mixed in a predetermined ratio to form an extraction solution, followed by filtration and evaporation, and thus a detailed description thereof will be omitted. .

The mixing ratio of the dried product of the second step of the present embodiment is 0.5 to 10% by weight, marigold 5 to 40% by weight, 5 to 20% by weight in yam, samil 0.5 to 10% by weight, green tea leaves 5 to 20% by weight, chajeoncho 5 It is preferable to form the respective mixed dry matter in the range of the ratio of -40 wt%, weak hair 5-40 wt%, bird vine 5-20 wt%, honeysuckle 5-30 wt%, licorice 0.5-10 wt%.

Experiments were performed to compare the yields of the herbal extracts according to the various solvents used in the preparation method of the herbal extracts according to the present invention.

① Experiment Method

The experiment was performed by adding 2 L of solvent per 100 g of the dried substance and the extract, and the dried substance and the extract and the solvent were heated under atmospheric pressure for 2 hours in a mantle provided with a reflux condenser, and the residue on top of the heated mixture was removed. The liquid residue in the lower part was filtered and each extract obtained from the dried product and the mixed extract obtained from the extract were obtained as the obtained product.

The respective extracts and mixed extracts were evaporated under a condition of 0.008 atm (5 mmHg) and 50 ° C. to finally obtain dry extracts and mixed extracts of each dry extract.

② Experiment Result

The amount of the dry extract and the dry extract of the mixed form obtained by each type of solvent was high in yield when water, ethanol and methanol were added as a solvent. It is effective to use water and ethanol as solvents.

Table 1 shows the yield values obtained for each type of solvent.

TABLE 1

Type of solvent Yield (%) Type of solvent Yield (%) water 10 Acetone 3 ethanol 8 chloroform 4 Methanol 8 cyclo-butanol 4 Isopropyl Alcohol 6 cyclo-pentane 2 n-butanol 6 n-nucleic acid 2 Ethyl acetate 7

Experiments performed to explain the effect of the herbal extract prepared by applying water and ethanol confirmed from the results of Table 1 and the results are described in detail below.

end. Test of nicotine concentration in blood

1. Experimental conditions

① Test Animal

     ICR Mouse of Wister strain male rats (6 weeks old)

② Test environment

     Five animals were housed in a wire cage in an animal room set at room temperature 24 ± ° C., humidity 55 ± 5%, ventilation number 12 times / hour, lighting time 12 hours / day (light at 8 am, light at 8 am). The feed was made free of solid feed with water.

③ Method of administration

     Adapted to the test environment for 2 weeks, animals were tested at 8 weeks of age. The test substance was orally administered by mixing the herbal extract obtained in Example with a solid feed. The herbal extracts obtained from the examples were mixed in 0.5ml / mari solid feed and orally administered to the body as free food with water.

④ Dosing time

Administration was performed at 2 pm and the period was 7 days. At the end of dosing and at the end of the test, 1 ml of blood was drawn from the heart as a sample for nicotine measurement.

⑤ Observation method

Dosing days were observed immediately after administration and in the evening, and the morning and evening thereafter. Observation was carefully conducted from outside the breeder.

2. Experimental Results

① nicotine concentration in blood

Blood was adjusted to pH 9 with dilute ammonia water. Next, the resultant was maintained on an outer column (EXTRELUT COLUMN; manufactured by Merck Co., Ltd.) for 15 minutes, eluted with 15 ml of ethyl acetate, the eluted acetate was distilled off under reduced pressure, and 100 µl of ethyl acetate was added to the resulting residue. The sample was analyzed.

The analysis was performed using gas chromatography (HP 5890 SERIESП, FID, USA) with 2% Tharmon 1000 + 1% KOH chromosorb WAM (8 / 100Mesh). A glass column 2 m long and 3 mm in diameter was used.

The column temperature was 100 ° C., the carrier gas used nitrogen (N ₂ ), and the flow rate was 60 ml / min. Table 2 shows the result of administering the herbal extract obtained from the example for 7 days, and the nicotine concentration was 30 mg / kg / 10ml and was intraperitoneally administered.

TABLE 2

Paralysis symptoms Activity start time Activity end time Death time Nicotine concentration in blood (㎍ / ㎖) G1 One 27 ' 2'10 ' 5'50 ' recovery 0.024 2 36 ' 2'44 ' 6'10 ' recovery 0.027 3 43 ' 3'25 ' 6'51 ' recovery 0.015 4 43 ' 3'50 ' 6'56 ' recovery 0.021 5 33 ' 3'52 ' 6'35 ' recovery 0.017 G2 One 22 ' 4'42 ' 6'45 ' 31'18 ' 1.037 2 26 ' 4'46 ' 6'35 ' recovery 0.014 3 20 ' 5'40 ' 7'25 ' recovery 0.012 4 24 ' 5'47 ' 7'20 ' recovery 0.013 5 23 ' 4'46 ' 6'47 ' recovery 0.019 G3 One 23 ' 35 ' 56 ' 1'33 ' 12.178 2 33 ' 45 ' 1'15 ' 1'35 ' 12.183 3 29 ' 31 ' 1'20 ' 1'50 ' 12.987 4 25 ' 54 ' 1'35 ' 1'57 ' 12.969 5 22 ' 51 ' 1'50 ' 2'10 ' 11'719 G4 One 33 ' 2'50 ' 4'20 ' 6'15 ' 4.019 2 25 ' 2'40 ' 4'50 ' 7'18 ' 3.679 3 24 ' 2'45 ' 4'47 ' 6'45 ' 4.095 4 21 ' 2'37 ' 5'10 ' 6'25 ' 4.074 5 33 ' 2'35 ' 4'15 ' 6'54 ' 4.117

Note-G1: Nicotine administration after pretreatment of the herbal extract obtained in Example.

G2: After pretreatment of the herbal extracts obtained from the examples, administration of the herbal extracts obtained from the examples after nicotine administration.

G3: control nicotine administration.

G4: Administration of the herbal extract obtained from the Example after administration of the control nicotine.

The test population was tested five dogs in each group

② autopsy

At the end of dosing, there was no gross abnormality in each individual when recovered from necropsy, but gross abnormalities were found in each individual at death.

I. Measurement experiment of urine nicotine concentration

The urine nicotine concentration was measured in the same manner as the test animal, test environment, administration method, administration time, observation method, and analysis method of Experiment 1, and the results of the measurement test of nicotine concentration in urine are described below.

Test result of nicotine concentration in urine was adjusted to pH 9 by adding dilute ammonia water to urine, maintained for 1 minute in an EXTERELUT COLUMN (manufactured by Merck), eluted with 15 ml of ethyl acetate, and then eluting acetate was Distilled off. 100 µl of ethyl acetate was added to the resulting residue to dissolve and used as an analytical sample. Table 3 shows the results after administration of the herbal extract prepared according to this example for 7 days, and was administered nicotine concentration 30mg / kg / 10ml intraperitoneally.

TABLE 3

Paralysis symptoms Activity start time Activity end time Death time Nicotine Concentration in Urine (µg / mL) G1 One 22 ' 2'20 ' 5'30 ' recovery 5.524 2 34 ' 2'34 ' 6'30 ' recovery 5.527 3 43 ' 3'45 ' 6'31 ' recovery 5.515 4 46 ' 3'40 ' 5'36 ' recovery 5.531 5 30 ' 3'32 ' 6'15 ' recovery 5.517 G2 One 23 ' 4'32 ' 6'35 ' recovery 5.537 2 23 ' 4'36 ' 6'55 ' recovery 5.514 3 34 ' 5'50 7'55 ' recovery 5.512 4 21 ' 5'17 ' 7'55 ' recovery 5.513 5 20 ' 4'26 ' 6'37 ' recovery 5.519 G3 One 20 ' 30 ' 57 ' 1'43 ' 0.021 2 33 ' 55 ' 1'35 ' 1'55 0.031 3 23 ' 41 ' 1'50 ' 2'30 ' 0.037 4 24 ' 54 ' 1'35 ' 1'57 ' 0.021 5 25 ' 55 ' 1'55 ' 2'30 ' 0.029 G4 One 31 ' 2'30 ' 4'10 ' 6'45 ' 0.219 2 23 ' 2'30 4'45 ' 7'25 ' 0.279 3 23 ' 2'55 ' 4'37 ' 6'15 ' 0.215 4 23 ' 2'47 ' 5'50 ' 6'55 ' 0.214 5 30 ' 2'55 ' 4'45 ' 6'14 ' 0.217

All. Carbon monoxide concentration test in blood

1. Experimental conditions

① Test Animal

ISTER Mouse of Wister strain male rats (6 weeks old)

② Test environment

Five animals were housed in a wire cage in an animal room set at room temperature 24 ± 1 ° C., humidity 55 ± 5%, ventilation number 12 times / hour, lighting time 12 hours / day (lighting up at 8 am, turning off at 8 pm). . Feed was freely fed solid feed with water.

③ Method of administration

Adapted to the test environment for 2 weeks, animals were tested at 8 weeks of age. The test substance was orally administered by mixing the herbal extract obtained in Example with a solid feed. The herbal extracts obtained from the examples were mixed in 0.5ml / mari solid feed and orally administered to the body freely with water.

④ How to expose carbon monoxide (C0)

On the day after the end of the administration, each animal was housed in an exposure chamber (Sugiyamage Co., Ltd.), and a mixed gas of 21% O ₂ and 100 ppm CO was introduced into the pump and inhaled for 5 minutes. The concentration in the chamber was examined with a carbon monoxide meter (COM-4, manufactured by Komei Rikagaku Kogyo, Japan).

⑤ Measurement of carbon monoxide-hemoglobin (CO-Hb) concentration in blood

Immediately after the end of the exposure, blood was drawn from the heart, 0.5 ml of blood was placed in a 5 ml reaction vial, and a drop of octyl alcohol and 0.25 ml of saturated aqueous potassium ferricyanate solution were added. It was used as a sample for gas phase analysis.

For analysis, gas chromatography (US 5890 SERIESП, TCD attached) was used, and a column of 2 m in length and 3 mm in diameter filled with molecular sieve 5A (3 / 60Mesh) was used. The column temperature was 60 ° C., the carrier gas used hydrogen (H ₂ ), and the flow rate was 50 ml / min.

2. Experimental Results

① the concentration of carbon monoxide in the blood

The results of measuring the concentration of carbon monoxide (CO) in the blood after the test, see Table 4, Table 4 is the result of administration of the herbal extract prepared according to the present embodiment for 7 days.

 TABLE 4

  CO-Hb concentration in blood (%) G1 One 53.8 2 52.9 3 50.3 4 52.8 5 53.5 G2 One 53.0 2 52.8 3 53.5 4 52.9 5 53.0 G3 One 69.3 2 70.2 3 69.1 4 68.5 5 68.9 G4 One 61.5 2 60.8 3 62.9 4 62.1 5 61.8

② autopsy

At the end of the autopsy, no gross abnormalities were found in each individual in each group.

la. Test for Tar

1. Experimental conditions

① Test Animal

ISTER Mouse of Wister strain male rats (6 weeks old)

② Test environment

Five animals were housed in a wire cage in an animal room set at room temperature 24 ± 1 ° C., humidity 55 ± 5%, ventilation number 12 times / hour, lighting time 12 hours / day (lighting up at 8 am, turning off at 8 pm). . Feed was freely fed solid feed with water.

③ Method of administration

Adapted to the test environment for 2 weeks and animals were tested at 8 weeks of age. The test substance was orally administered by mixing the herbal extract obtained in Example with a solid feed. The herbal extracts obtained from the examples were mixed in 0.5ml / marion solid feed and orally administered to the body as free food with water. Tar was administered into the stomach using a Stomach tube.

④ Observation Method

Dosing days were observed immediately after administration and in the evening and morning and evening from the following day. Observation was carefully conducted from outside the breeder.

2. Experimental Results

The result of measuring the Tar after the experiment is shown in Table 5, and Table 5 is the result after administration of the herbal extract prepared according to the present Example. The concentration of Tar was administered intraperitoneally at 30 mg / kg / 10 ml.

TABLE 5

time 1 day 2 days 3 days 4 days 5 days 6 days 7 days G1 One survival survival survival survival survival survival survival 2 survival survival survival survival survival survival survival 3 survival survival survival survival survival Dead " 4 survival survival survival survival survival survival survival 5 survival survival survival survival survival survival survival G2 One survival survival survival survival survival Dead " 2 survival survival Dead " " " " 3 survival survival survival survival survival survival survival 4 survival survival survival Dead " " " 5 survival survival survival survival survival Dead "

References: G1: Pre-treatment by mixing the herbal extracts obtained from the example into a general feed and then administering Tar.

G2: Tar administration while the control normal feed.

The test population was tested five dogs in each group

hemp. Antioxidant Test

1. Test Method

Antioxidant activity was tested by the method of Kiharu, adding 2mg of linoleic acid to 1ml of distilled water, 10mg of Tween-20 to 1ml of distilled water, and 0.2M aerated for 1 hour. 1.0 ml of potassium phosphate (pH 7.4) was added to the crude herb extract obtained from Example to prepare a mixture. The mixture was reacted at 37 ° C. for 24 hours, taking 0.1 ml of the reaction solution at 3 hour intervals, adding 9.7 ml of 75% ethanol, 0.1 ml of 30% Ammonium thiocyanate and 0.02 M 3.5% of ferrous ammonium sulfate and 0.1 ml of hydrochloric acid were added and the absorbance was measured at 500 nm after 3 minutes.

2. Results

The antioxidant power measurement results of the herbal extracts obtained from the examples are shown in Table 6 and Table 7. As a control, the synthetic antioxidant BHT (dibutyl hydroxy toluen) and the natural antioxidant alpha-tocopherol (α-Tocopherol) Was used.

Table 7 shows the measurement results of the antioxidant power, and Table 6 summarizes the average values of the antioxidant powers measured from Table 7. As shown in Table 6, the herbal extracts obtained from the examples showed higher antioxidant power than alpha-tocopherol and were slightly lower than BHT. In addition, as a result of measuring the antioxidant power using the herbal extract obtained from the Example was found to show a high difference with the control (control).

TABLE 6

0 hr 3 hrs 6 hrs 9 hrs 12 hrs 21 hrs 23 hrs 30 hrs Control 0.042 0.086 0.169 0.234 0.296 0.502 0.557 0.779 Herbal Extract 0.043 0.059 0.056 0.061 0.062 0.064 0.064 0.060 Tocopherol 0.042 0.094 0.127 0.137 0.144 0.277 0.346 0.546 BHT 0.041 0.044 0.043 0.045 0.043 0.046 0.046 0.045

TABLE 7

0 hr 3 hrs 6 hrs 9 hrs 12 hrs 21 hrs 23 hrs 30 hrs  Control 0.041 0.085 0.167 0.229 0.291 0.506 0.555 0.800 0.042 0.085 0.167 0.234 0.290 0.494 0.556 0.787 0.042 0.087 0.172 0.238 0.308 0.506 0.559 0.751 Average 0.042 0.086 0.169 0.234 0.296 0.502 0.557 0.779  Herbal Extract 0.043 0.058 0.056 0.059 0.062 0.065 0.062 0.060 0.041 0.059 0.057 0.062 0.062 0.063 0.063 0.059 0.045 0.061 0.056 0.062 0.061 0.063 0.063 0.059 Average 0.043 0.059 0.056 0.061 0.062 0.064 0.064 0.060  Tocopherol 0.041 0.092 0.129 0.139 0.145 0.276 0.352 0.537 0.044 0.099 0.126 0.137 0.144 0.278 0.343 0.552 0.041 0.092 0.127 0.135 0.144 0.276 0.344 0.548 Average 0.042 0.094 0.127 0.137 0.144 0.277 0.346 0.546  BHT (BHT) 0.041 0.045 0.042 0.047 0.043 0.045 0.045 0.045 0.041 0.043 0.043 0.045 0.044 0.047 0.046 0.045 0.040 0.045 0.043 0.045 0.043 0.045 0.048 0.046 Average 0.041 0.044 0.043 0.045 0.043 0.046 0.046 0.045

bar. Plasma Assay

Plasma was measured by total cholesterol measurement, blood fat measurement, lipid peroxidation content measurement. Three-week-old SD-rat was divided into groups of normal, nicotine and nicotine plus herbal extracts (n = 7) and sacrificed at 7 weeks of age.

Normal (n = 6): Free expression.

Nicotine (n = 8): Nicotine 4 mg / kg BW oral administration + 200 mg / kg BW physiological saline intraperitoneal administration.

Nicotine + herbal extract (n = 7): Nicotine 4 mg / kg BW oral administration + 200 mg / kg BW The herbal extract obtained from the Example is intraperitoneally administered.

1. Plasma Analysis

Blood was collected from the arteries of the sacrificed animals and treated with EDTA to prevent coagulation. Collected blood was centrifuged at 3,000 rpm for 10 minutes to separate serum and stored at -70 ℃ until analysis.

1) Total Cholesterol: Total absorbance was measured by measuring the absorbance at 505 nm of UV-VIS Spectrophotometer using blood total cholesterol measurement kit (Wako, Japan).

2) Triglyceride: Triglyceride in blood was measured using a blood triglyceride kit (Wako, Japan) and quantified by measuring absorbance at 410 nm of UV-VIS Spectrophotometer. The experimental results are shown in Table 8.

TABLE 8

Plasma (mg / dl) Normal Nicotine Nicotine + Herbal Extract Triglyceride 37.2 ± 6.0 a 77.1 ± 3.8 b 46.3 ± 2.7 a Total cholesterol 89.8 ± 3.2 c 68.0 ± 1.9 b 43.7 ± 3.0 a glucose 151.5 ± 2.4 a 174.4 ± 2.7 b 197.3 ± 3.5 c

2. Determination of Lipid Peroxidation Content (TBARS)

After administering nicotine and herbal extracts obtained from Examples for 4 weeks, the liver was extracted by anesthesia with diethyl ether. The extracted liver was stored at -70 ° C until analysis. 1 g of liver was homogenized with 100 mM trichloric acid (Tris-HCl), pH 7.4 buffer, and TBA color reagent was added to quantitatively analyze the TBARS content at 535 nm. The results are shown in Table 9. TBARS is Thiobarbituric Acid-Reactive Substance.

Lipid peroxidation was measured by measuring the lipid peroxidation product (MDA-MalonDiAldehyde) reacting with TBA reagent.

TABLE 9

TBARS (nmol / mg) Normal Nicotine Nicotine + Herbal Extract Liver 1.0 ± 0.0 b 0.91 ± 0.0 a 0.8 ± 0.1 a Brain 2.2 ± 0.1 2.0 ± 0.1 2.1 ± 0.1 Lung 1.5 ± 0.1 1.5 ± 0.1 1.5 ± 0.1 Plasma 0.3 ± 0.0 0.3 ± 0.0 0.3 ± 0.1

four. Test for increased immunity

1. Test Method

Spleens extracted from 3 week old ISR mice were washed with physiological saline to separate splenocytes from complete RPMI 1640 media. The separated splenocytes were dispensed at 96 x wells at 2 x 105 cells / ml and incubated with stimulators such as Con A and LPS to different concentrations of H. After treatment with HAT, the cells were incubated for ₂ days at 37 ° C. in a 5% CO ₂ incubator and treated with 20 μl / well of alarmar blue to absorb the absorbance with a micro plate reder. Measured.

* Herbal extract concentrations obtained from the examples: 20, 50, 100, 200 ug / ml

* All experiments were performed in a clean bench, and all samples including media were filtered (22 μm) and treated to cells.

Micro plate reder: Measurements were measured at 570 nm and reference 600 nm.

Alamar Blue Assay: Alamar Blue Dye is a cytotoxic assay that uses non-toxic metabolism by cell activity to turn blue and is a mitoplate reader. If the OD measured by the micro plate reder is low, the cell proliferation is evaluated to be reduced.

2. Results

After stimulating the control A (Control A) was added to the Alama blue (alamar blue) after 2 days of incubation, the results of the injection of the herbal extract obtained from the Example in the state of 32 hours has been shown in Table 10.

TABLE 10

Average Standard error Normal -0.05 0.01 Control 0.09 0.01 Herbal Extract 200 0.14 0.01 Herbal Extract 100 0.13 0.01 Herbal Extract 50 0.11 0.01 Herbal Extract 25 0.08 0.01

Ah. Tests for Smoking-Related Biological Activities

Four weeks old asial mice (ICR mice) were used in the experiment after one week of adaptation. The animal breeding room was set at room temperature 24 ± 1 ° C., humidity 55 ± 5%, ventilation number 12 times / hour, and lighting time 12 hours / day (light at 8 am, light at 8 am). Feed and water were taken freely during the experiment.

Samples of the herbal extracts obtained from the examples were administered orally twice a day at 10 o'clock and 17 o'clock, and the daily dose of the sample measured the body weight of rats every week to determine the daily intake and The weight was calculated from the ratio of 3/4), and the average human weight was calculated as 65 kg.

1. Nicotine excretion test

The urine of mice was bred in a metabolism cage. At 14 o'clock, nicotine dissolved at a concentration of 1 mg / ml in sterile distilled water for injection was intraperitoneally administered 10 mg / kg of body weight. The urine was collected at 14 o'clock the next day and nicotine concentration was measured by gas chromatography.

The pH was adjusted to 9 by adding dilute ammonia water to the urine.

The resultant was maintained for 15 minutes on an Extreme column (manufactured by Merck), eluted with 15 ml of ethyl acetate, and eluted with distillation under reduced pressure. 100 µl of ethyl acetate was added to the residue to dissolve the sample.

2m length filled with Gas chromatography (Hewlett Packard 5890, FID) and 2% Tharmon 1000 + 1% Potassium Hydroxide (KOH) Chromosorb WAM (8 / 100Mesh) A 3 mm glass column was used, the column temperature was 100 ° C., the carrier gas was nitrogen (N ₂ ), and the flow rate was performed at 60 ml / min.

2. Tar detoxification test

Tar dissolved at a concentration of 3 mg / ml in sterile distilled water for injection was intraperitoneally administered 30 mg per kg of body weight and the survival rate after 7 days was examined.

3. Plasma Total Oxidation Test

After oral administration of the herbal extracts obtained from the examples for 3 weeks, mice were anesthetized with ether and blood was collected from the heart to separate plasma from test tubes containing anticoagulants. Plasma total oxidative power was measured using a kit for measuring total antioxidant status of Randox (U.K.).

4. Statistical Processing

The measurements were analyzed by student's t-test and expressed in M ± SEM.

5. Results and Discussion

① Nicotine excretion test results

If you stop smoking, the nicotine accumulated in your body begins to decrease. During this period, it is very difficult to tolerate the body's desire for nicotine, which is addicted to nicotine. It is very important to get rid of nicotine accumulated in your body quickly in order to succeed in quitting smoking. It is known to be common to all addictive symptoms.

Table 11 examines the effect of ingestion of herbal extracts obtained from Examples on urinary excretion of nicotine in ICR mice.

 TABLE 11

Control Herbal Extract Urinary nicotine excretion (ug / day) excreted in the urine 0.217 ± 0.029 3.758 ± 0.523 ^*

( ¹ Values are mean ± SEM), n = 6.

* P <0.05 for the comparison with control group.

The urine nicotine excretion was increased by 17.3 times in the intake group of the herbal extract obtained from the example compared to the control group.

② Result of Tar detoxification function test

Table 12 examines the effects of intake of herbal extracts obtained from the examples on the toxicity of Tar in ISR mice.

TABLE 12

Control HAT Survival ratio 1/10 7/10

¹ n = 10.

In the control group, one out of ten tar-addicted rats survived, whereas in the administration group to which the herbal extract obtained from the Example was administered, seven survived, and the herbal extract obtained from the Example was treated by tar poisoning. Significantly reduced mortality. The above results show that the herbal extracts obtained from the examples effectively excrete nicotine and reduce the toxicity of tar. Therefore, the herbal extracts obtained from the examples are supposed to act to enhance metabolism and physiological activity related to the removal of other toxic substances such as nicotine and tar accumulated in the body due to smoking.

Oxygen is an essential element for organisms doing organic hops, but active oxygen generated during incomplete combustion during energy metabolism destroys and destroys homeostasis of cells by denaturing and destroying macromolecules in cells.

Active oxygen is generated by factors such as tobacco and soot, and damages biological components such as proteins, DNA, enzymes, and T-cells to cause various diseases. Especially, unsaturated fatty acids, which are components of biological membranes, It is known to attack and produce lipid peroxide, causing aging and adult disease.

③ Plasma total oxidation test result

Table 13 shows the results of measuring total antioxidant status by separating plasma from the animals administered with the herbal extracts obtained from the examples. Table 13 compares the total oxidative powers of plasma in rats treated with functional foods for three weeks.

The total oxidative power of the group administered with the herbal extract obtained from the Example increased by 38.8% compared to the control group and there was a significant difference. (P <0.05) The antioxidant power of plasma may be considered as a representative value of the antioxidant power in the body, and the plasma Increasing the total oxidative power of means that the antioxidant power in the body was increased by administration of the herbal extract obtained from the example.

Green tea containing antioxidants of polyphenols such as catechin has also been reported to increase plasma antioxidant activity. These results show that the herbal extract obtained from the example can be used as an additive to increase the biological antioxidant power of the herbal extract that protects the living body from diseases caused by oxidative damage caused by factors such as smoking.

TABLE 13

Relative Trolox ² concentration (n mol / ml plasma) Control 1.21 ± 0.05 Herbal extract obtained from the example 1.68 ± 0.08 *

( ¹ Values are mean ± SEM), n = 8.

( ² Water-soluble vitamin E analogue)

* P <0.05 for the comparison with control group.

 From the above experimental results, when the herbal extract of the present invention is administered to the human body and at the same time nicotine is administered, the effect of lowering the amount of nicotine in the blood and the release of nicotine in the urine is shown. In addition, exposure to carbon monoxide has the effect of lowering the concentration of CO-hemoglobin in the blood. In addition, high antioxidant power and total cholesterol, blood fat, lipid peroxidation effect is shown to increase the body's immunity and has the advantage to increase the immune system against disease.

The herbal extract of the present invention can be expected to have a preventive effect against various diseases that may occur due to smoking by removing the harmful components in the human body that may occur due to smoking, including nicotine in the blood.

In addition, the herbal extract of the present invention provides an advantage that can be easily added to the functional food to be prepared and supplied in various forms of preparation.

Claims (8)

A first step of drying the aloe, marigold, camellia, samchil, green tea leaf, weak wheat, bird vine, honeysuckle, licorice and chajeoncho respectively to form a dry matter; A second step of forming an extract solution in which the solvent is added to each dried product of the first step and heated to extract the components of the dried product into the solvent; A third step of removing the solid material by filtering the extraction solution of the second step; A fourth step of obtaining each extract by evaporating the solvent component contained in the filtered extract solution of the third step; And The fifth step of providing a mixed extract by mixing the extract of the fourth step in a predetermined ratio; Method of producing a herbal extract to remove the harmful components due to smoking, characterized in that it comprises a. A first step of drying the aloe, marigold, camellia, samchil, green tea leaf, weak wheat, bird vine, honeysuckle, licorice and chajeoncho respectively to form a dry matter; A second step of mixing the dry matter of the first step at a predetermined ratio to provide a mixed dry matter; A third step of adding a water-soluble solvent to the mixed dry matter of the second step to form a mixed extractive solution in which the dry ingredient is extracted into the solvent; A fourth step of removing the solid material by filtering the mixed extraction solution of the third step; A fifth step of providing a mixed extract by evaporating the solvent component contained in the mixed extract solution filtered in the fourth step; Method of producing a herbal extract to remove harmful components due to smoking. The method according to claim 1 or 2, The mixing ratio of each extract and each dried product is 0.5 to 10% by weight of aloe, 5 to 40% by weight of marigold, 5 to 20% by weight of yam, samil 0.5 to 10% by weight, 5 to 20% by weight of green tea leaves, 5 to 40 of chajeoncho. Weight%, weak hair 5-40% by weight, fresh ginseng 5-20% by weight, licorice 0.5-10% by weight of the herbal extract for removing harmful ingredients due to smoking, characterized in that. The method according to claim 1 or 2, The solvent is water, lower alcohols, esters, ketones, aliphatic hydrocarbons, chloroform at least one selected from the group consisting of medicinal extracts for the removal of harmful ingredients due to smoking. The method according to claim 1 or 2, The step of evaporating the solvent component is a method for producing a herbal extract for removing harmful ingredients due to smoking, characterized in that it comprises a step of heating to a temperature of 40 to 60 ℃ at 0.005 to 0.1 atm. The method according to claim 1 or 2, Mixing the herbal extract in a ratio of 5 to 70% by weight based on the conventional food ingredients to provide a functional food, characterized in that the manufacturing method of the herbal extract for removing harmful ingredients due to smoking. The method according to claim 1 or 2, Mixing the herbal extract with an excipient comprising lactose, corn starch, cellulose, magnesium stearate to prepare in the form of powder, granules, tablets or capsules for the removal of harmful components due to smoking Method of manufacturing herbal extracts. 0.5-10% by weight of aloe, 5-40% by marigold, 5-20% by weight of yam, 0.5-10% by weight of samchi, 5-20% by weight of green tea leaves, 5-40% by weight of chajeoncho, 5-40% by weight of wild buckwheat, Herbal extract for removing harmful ingredients from smoking, characterized in that 5 ~ 20% by weight and 0.5 ~ 10% by weight licorice.