KR20030087728A - Composition for detoxifying toxic substances having Aloe vera gel and propolis - Google Patents

Abstract

PURPOSE: A composition containing an aloe vera gel and propolis detoxifying effect is provided. Therefore, it promotes the excretion of benzo(a)pyrene, nicotine or the like, exhibits an excellent effect on the study in a biological indicator of factors related with blood disease and can detoxify toxic material caused by various pollutants, environmental hormone and smoking. CONSTITUTION: A composition for detoxifying toxic material contains 10 to 90% by weight of aloe vera and 90 to 10% by weight of propolis, based on the total weight of the composition. It also reduces a disease indicator in blood such as creatinine, bilirubin or glucose. It is a food composition selected from the group consisting of a beverage, tea, ice cream, candy, jelly, bread, rice cake and noodles. It is administered in an amount of 10 to 5,000mg/day.

Description

Composition for detoxifying toxic substances having Aloe vera gel and propolis

The present invention relates to a composition for detoxifying toxic substances comprising aloe vera gel and propolis, and more particularly to a composition capable of detoxifying toxic substances caused by smoking.

The biggest problem in modern society is the disease caused by smoking toxic substances. For example, various diseases caused by tobacco are caused by more than 4,000 harmful toxins (including carcinogens). In recent years, smoking has threatened the health of not only direct smokers but also secondhand smokers, so smoking is a problem for all of us. Smoking accounts for 30% of the total population dying from cancer, with 85% of male lung cancer deaths and 80% of female lung cancer deaths. Cancers caused by tobacco are diverse, including cervical cancer and leukemia in women. In addition, it causes gastrointestinal disease, heart disease, stroke and atherosclerosis. Therefore, when the detoxification by inhibiting the action of tobacco toxic substances or by promoting the excretion can be prevented in advance a number of diseases caused by smoking.

Benzo [a] pyrene (BaP), a representative toxic substance caused by smoking, is a harmful carcinogen, and nicotine is not only a precursor for producing tobacco-specific carcinogens such as NNK, but also the habit of tobacco And various toxic effects.

Benzo [a] pyrene, a toxic substance of tobacco, is also a pollutant contained in soot or automobile exhaust. With the development of the industrial society, environmental pollution due to the concentration of the population is increasing day by day, and the pollutants around the incinerators and the exhaust emissions from automobiles, industrial complexes, etc. are exacerbating environmental conditions through various forms and routes. . Among the endocrine obstructions (aka environmental hormones), which are emerging recently, a lot of pollutants are derived from the environment, and some of them may cause problems in the human endocrine system. Automobile exhaust is also a collection of carcinogens, and the gas produced by burning fuel contains a number of toxic benzo [a] pyrenes, which are particularly representative of toxic substances. It is known to make a substance. Even small doses of benzo [a] pyrene are always at high risk for lung cancer, liver cancer, stomach cancer, bladder cancer, esophageal cancer and skin cancer.

In addition, the toxic substance increases blood levels of creatinine, glucose, and bilirubin, which are factors related to the disease of blood, and the increased concentration of the factors causes disease in the human body.

Creatinine is a waste product produced by the breakdown of creatine after protein is used as an energy source in muscles. It is discharged into the blood and then filtered out of the kidneys. Creatine and creatinine are filtered out of the glomeruli, but creatine is completely reabsorbed in the tubules, whereas creatinine is not resorbed in the tubules and is secreted from the tubules when blood levels are high. For this reason, blood creatine concentration is considered clinically important as a measure of renal function. In recent years, many diabetes mellitus in adults is mainly insulin-independent diabetes. Appears and is determined by measuring the level of glucose in the blood. In the case of bilirubin (bilirubin), in the case of prehepatic jaundice or hepatic jaundice, blood concentration increases.

Aloe is a medicinal plant belonging to the family Liliaceae and has been widely used in folklore since ancient times because of its various effects. The civilian has long used aloe as a suntan ointment, suntan powder, cosmetic cream, lotion and shampoo. However, scientific researches on the exact effects and mechanisms of action of aloe, which have been used for a long time, have been made relatively recently. Recent studies on aloe have been reported to show a variety of activities such as wound healing, anti-inflammatory activity, ulcer treatment activity, anticancer activity, antimicrobial activity, immunomodulatory activity, cell growth promoting activity.

Propolis is known as the product of honey bees made by honey bees scraping their hind legs and mixing them with the saliva of honey bees to protect the epidermis and shoots of plants. Propolis is known to have effects such as anti-inflammatory, antibacterial, antioxidant, local anesthesia, antiviral, cell regeneration and tissue regeneration.

Although several effects of aloe and propolis are known as described above, no literature has reported that these substances have a detoxifying effect.

The inventors have intensively studied aloe and propolis, and as a result, the composition comprising aloe vera gel and propolis has been found to contain toxic substances, in particular toxic substances generated from smoking or automobile emissions such as benzo [a] pyrene. The first discovery with detoxifying action was completed the present invention.

It is an object of the present invention to provide a detoxifying composition comprising aloe vera gel and propolis.

1 is a graph showing the excretion effect of benzo [a] pyrene on in vivo ( in vivo ) for administration of the composition of the present invention,

Figure 2 is a graph showing the excretory effect of cotinine in vivo on the administration of the composition of the present invention,

3 is a graph showing the blood concentration of creatinine in vivo for administration of the composition of the present invention,

Figure 4 is a graph showing the blood concentration of in vivo complementary glucose for administration of the composition of the present invention,

5 is a graph showing the blood concentration of bilirubin in vivo in the composition administration of the present invention,

The present invention provides a composition for detoxifying toxic substances, including aloe vera gel and propolis.

In the composition of the present invention, it is preferable that the content of aloe vera gel is 10 to 90% by weight, and the content of propolis is 90 to 10% by weight based on the total weight of the composition. When the mixing ratio is out of the range, the effects of the components of aloe vera gel and propolis cannot be expected, and an additive or synergistic effect of detoxification of these toxic substances cannot be expected, which is not preferable.

In particular, the present invention provides a composition for detoxifying a toxic substance which can detoxify a toxic substance caused by smoking. The toxic substance is, for example, benzo [a] pyrene, nicotine, tar and the like.

The present invention also provides a composition for detoxifying a toxic substance, which reduces blood disease markers. The blood disease indicator is creatinine, bilirubin or glucose and the like.

The present invention also provides a food composition having a toxic substance detoxifying effect including aloe vera gel and propolis.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

The first aspect of the present invention is to provide a composition for detoxifying toxic substances comprising aloe vera gel and propolis.

The aloe vera gel included in the composition of the present invention is preferably a gel obtained from aloe vera alone, or powdered aloe vera gel, or a mixture of aloe vera gel and powder in a suitable ratio. Can be used. In addition, a commercially available aloe product containing a large amount of the high physiological activity effect of aloe may be used, and in particular, active aloe ^TM (Aloecorp, USA) and the like may be used. The active aloe ^TM is a product containing more of the middle range of polysaccharides with the highest bioactive activity of aloe than aloe vera, and is 200 times concentrated powder of aloe vera gel.

The propolis contained in the composition of the present invention can be used commercially available propolis, it is also possible to use a product that has a large amount of components with high physiological activity effect by processing the commercial propolis.

The composition of the present invention may contain only aloe vera gel and propolis, but may include a pharmaceutically acceptable carrier or the like for ease of administration.

The compositions of the present invention may be formulated by methods known in the pharmaceutical art, and may be mixed with themselves or with a pharmaceutically acceptable carrier, excipient, and the like to be a pharmaceutically conventionally acceptable pharmaceutical agent such as a liquid or a syrup. , Capsules and the like, which can be administered orally or parenterally. The composition of the present invention is preferably administered orally as a powder.

The liquid, capsules and the like containing the composition of the present invention is preferably used as a health functional food, the term "health functional food" used in the present invention is used as a raw material or ingredient having a useful functionality to the human body Refers to food products manufactured and processed in the form of tablets, capsules, powders, granules, liquids, and pills.

The composition of the present invention is appropriately selected according to the absorption of the active ingredient in the body, the rate of excretion, the age and weight of the patient, the sex and condition, the severity of the disease to be treated, etc., but generally in adults 10 to 5,000mg / day, It is preferable to administer at 100 to 2000 mg / day. The unit dosage form so formulated may be administered several times at regular time intervals as needed.

Hereinafter, the present invention will be described for a smoker who is exposed to the most harmful toxic substance to the human body, preventing, inhibiting, promoting the excretion of harmful toxic substances or detoxification of the composition of the present invention, [a] Since pyrene is present not only in cigarette smoke but also in automobile exhaust, etc., the composition for detoxifying the toxic substance of the present invention is not limited to the detoxification of the toxic substance due to smoking.

As described above, benzo [a] pyrene, which is a representative toxic substance caused by smoking, is a harmful carcinogen, and nicotine is not only a precursor for producing tobacco-specific carcinogens such as NNK, but also has habits of tobacco and various toxic effects. Have.

The composition of the present invention exhibits detoxification by promoting the excretion of toxic substances such as benzo [a] pyrene and nicotine.

A second aspect of the present invention is to provide a composition for detoxifying a toxic substance, which reduces blood disease markers.

The composition of the present invention showed an excellent effect even in the study of biochemical indicators of factors related to the disease of the blood of the following experimental example. Thus, the composition of the present invention by lowering the blood concentration of factors associated with the disease of the blood, ie creatinine, glucose, bilirubin as described above to normalize the abnormal increase caused by the disease or disorder caused by the increase of these factors It can be expected to treat and prevent diseases.

A third aspect of the present invention is to provide a food composition having a toxic substance detoxifying effect including aloe vera gel.

The food composition is preferably a beverage, yogurt, tea, ice cream, candy, jelly, bread, rice cake or noodles, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to the following examples. These examples are for illustrative purposes of the present invention and are not intended to limit the protection scope of the present invention.

Example 1

42 g of aloe vera gel and 18 g (weight ratio 7: 3) of propolis were mixed in a conventional manner to prepare 60 g of the composition of the present invention. Commercial Aloe ^TM (Aloecorp, USA) was used as an Aloe Vera gel, and propolis used commercial Morica and propolis powders (Morikawa, Japan).

Example 2

60 g of the composition was prepared in the same manner as in Example 1, except that the mixing ratio of the aloe vera gel and the propolis was 3: 7 (18 g: 42 g).

Example 3

60 g of the composition was prepared in the same manner as in Example 1, except that the mixing ratio of the aloe vera gel and the propolis was 5: 5 (30 g: 30 g).

Example 4

66 g of the composition was prepared in the same manner as in Example 1, except that the mixing ratio of the aloe vera gel and the propolis was 10: 1 (60 g: 6 g).

Example 5 Preparation of Capsules

600 mg of the composition of Example 1

Lactose 50mg

Starch 50mg

Talc 2mg

Magnesium stearate appropriate amount

The capsules were prepared by mixing the above ingredients and filling gelatin capsules according to a conventional method for preparing capsules.

Example 6 Preparation of Capsules

600 mg of the composition of Example 2

Lactose 100mg

Starch 93mg

Talc 2mg

Magnesium stearate proper amount

The capsules were prepared by mixing the above ingredients and filling gelatin capsules according to a conventional method for preparing capsules.

Example 7 Preparation of Liquid

600 mg of the composition of Example 1

20 g of sugar

20 g of isomerized sugar

Lemon flavor

Add 100 ml of purified water

The above components were mixed according to a conventional method for preparing a liquid, and filled into 100 ml brown bottles and sterilized to prepare a liquid.

Example 8 Preparation of Liquid

1,200 mg of the composition of Example 3

20 g of sugar

20 g of isomerized sugar

Lemon flavor

Add 100 ml of purified water

The above components were mixed according to a conventional method for preparing a liquid, and filled into 100 ml brown bottles and sterilized to prepare a liquid.

Example 9 Preparation of Beverages

5% by weight active aloe ^TM, 3% by weight propolis (Morikawa, Japan) and 0.05% by weight food coloring, 0.05% by weight orange essence, 7.0% by weight fructose, 0.1% by weight citric acid, 0.05% by weight vitamin C A composition was prepared by adding a general functional beverage base, and then purified water was added to prepare a beverage.

Example 10 Preparation of Yogurt

1% by weight of active Aloe ^{TM and} 1% by weight of propolis (Morika, Japan) were added to a normal skim milk powder (Seoul Milk Products), followed by homogenization, followed by heat treatment at 80 ° C. for 30 minutes. After cooling to 37 ℃ yogurt strain inoculated with Lactobacillus Bulgaricus, fermented in a 37 ℃ thermostat for 12 hours, and then refrigerated at 4 ℃ to prepare the yogurt.

Experimental Example 1. Measurement of excretion of benzo [a] pyrene

For 10 smokers who smoke one or more packs of cigarettes daily, the composition of the present invention prepared in Example 1 was administered orally twice daily (morning and evening) at a dose of 600 mg / day for 1 month, followed by morning at weekly intervals. Urine was collected at 8-9 o'clock. Urine was collected in the same manner as above, with 10 smokers who smoked one or more packs of cigarettes per day not administered the composition of the present invention as a positive control group and 10 non-smokers as a negative control group.

To 4 ml urine sample 150 μl (200 ng / ml) of internal standard BaP-7,8-dihydrodiol (National Cancer Institute, Bethesda, MD) was added. 4 ml of chloroform was added thereto and shaken for 1 hour. Thereafter, it was centrifuged for 10 minutes (3,500 x g), and 3 ml of the organic phase was taken and dried with N ₂ gas. After dissolving in 300 μl methanol and filtering with a 0.45um filter to prepare a sample.

The sample (20 μl) was quantified benzo [a] pyrene using an HPLC system. HPLC analysis conditions are as follows.

1.HITACHI-Model L-7200 Autosampler; 2.Model L-7100 Pump; 3.Model L-7610 Degasser; 4.Model D-7000 / 6000 Interfadce Module; 5. Model FP-920 Fluorescence Detector (JASCO); 6. colume, Novapak C18 column (15 × 0.37 (i.d.)), 4um paricle, waters; 7. Solvent gradient 0 min-A solvent (80%), B solvent (20%), 20 min-A solvent (12%), B solvent (88%) (linear gradient) (A solvent-50: 50 (water : Methanol) / B solvent-methanol); 8. Spill rate-1.2 ml / min.

The amount of BaP was calculated by the height ratio by Fluorescence Detector (exi / emi: 265 / 450nm), and the unit was expressed in ng / ml, and the results are shown in FIG. 1. Statistical analysis examined the statistical significance of Bonferroni t-test and Dunnett's method of one-way analysis of variance (ANOVA) (*: p <0.05, **: p <0.01, ***: p <0.001).

As can be seen in Figure 1, the composition-administered group of the present invention was significantly (p <0.001) promoted the excretion of benzo [a] pyrene over 2, 3, 4 weeks after the composition administration compared to the control group.

Experimental Example 2. Measurement of excretion of cotinine

As in Experiment 1, the composition of Example 1 (600 mg / day) was administered to the smokers 2 group for 1 month, and urine was collected in the morning (8-9 hours) at weekly intervals.

2 ml NaOH (5 mol / L) was added to a 4 ml urine sample and 100 μl of 2-phenylimidazole (20 μg / ml. Stig. Louis, Mo.) was added as an internal standard. 4 ml of chloroform was added thereto and shaken for 1 hour. Thereafter, it was centrifuged for 10 minutes (3,500 x g), and 3 ml of the organic phase was taken and dried with N ₂ gas. After dissolving in 300 μl methanol and filtering with a 0.45um filter to prepare a sample.

Cotinine was quantified in the sample (20 μl) using the same HPLC system as in Experimental Example 1, and the results are shown in FIG. 2.

As shown in Figure 2, the composition-administered group of the present invention was significantly (p <0.001) promoted the excretion of cotinine from the 3 weeks after the composition administration compared to the smoking control, and significantly (p <0.001) 4 weeks after administration Excretion of cortinine was promoted.

As described above, the composition of the present invention promotes the excretion of nicotine, which is a metabolite of nicotine, and thus, promotes the excretion of nicotine, which is a poisoning component of tobacco.

Experimental Example 3 Measurement of Blood Levels of Creatinine, Glucose and Bilirubin

As in Experimental Example 1, the composition of Example 1 (600mg / day) was administered to the smokers 2 groups for one month, and blood was collected in the morning (8-9 hours) at weekly intervals.

The measurement of blood levels of creatinine, glucose and bilirubin is as follows.

(1) How to measure the concentration of creatinine

Blood was collected from the experimental group, centrifuged (3,000 rpm, 15 minutes), and the supernatant (serum) was separated. 15ul serum was injected and analyzed using Autoanalyzer Hitachi 7170. Jaffe Kinetic method was used for the analysis.

(2) measuring the concentration of glucose

Blood was collected from the experimental group, centrifuged (3,000 rpm, 15 minutes), and the supernatant (serum) was separated. 2.5ul of serum was injected and analyzed using Autoanalyzer Hitachi 7170. Assay, oxidase method was used.

(3) method for measuring the concentration of bilirubin

Blood was collected from the experimental group, centrifuged (3,000 rpm, 15 minutes), and the supernatant (serum) was separated. 4ul of serum was injected and analyzed using Autoanalyzer Hitachi 7170. Jendrassick-Cleghorn method was used for the analysis.

Plasma concentrations of creatinine, glucose and bilirubin of the sample (20 μl) were measured and compared with the control group, and the results are shown in FIGS. 3, 4 and 5.

As shown in FIG. 3, the composition-administered group of the present invention significantly decreased (p <0.05) the blood creatinine concentration at 2 weeks after the composition administration compared to the smoking control group, and also significantly at 3 and 4 weeks ( p <0.001) decreased.

In addition, as shown in Figure 4, the composition administration group of the present invention significantly reduced blood glucose concentration (p <0.05) compared to the smoking control group 1 week after the composition administration compared to the smoking control group, even at 2, 3, 4 weeks Significantly decreased (p <0.001).

In addition, as shown in Figure 5, the composition-administered group of the present invention significantly decreased (p <0.001) blood bilirubin concentration compared to the control group over 2, 3, 4 weeks after the composition administration compared to the smoking control.

3, 4, and 5, the administration of the composition of the present invention by lowering the blood concentration of all the factors associated with the disease of the blood, it can be expected to treat and prevent the disease caused by the increase of the factors .

As can be seen from the above, the composition of the present invention has an excellent toxic substance detoxifying effect by promoting the excretion of toxic substances such as benzo [a] pyrene, nicotine, etc., and also in studies of biochemical indicators of factors related to blood diseases. As a result, it is possible to detoxify toxic substances caused by various pollutions in modern society, toxic substances caused by environmental hormones and smoking.

Claims (9)

Composition for detoxifying toxic substances including aloe vera gel and propolis The composition of claim 1, wherein the content of aloe vera gel is 10 to 90% by weight, and the content of propolis is 90 to 10% by weight based on the total weight of the composition. The composition of claim 1 or 2, wherein the toxic substance is caused by smoking. The composition for detoxifying a toxic substance according to claim 1 or 2, wherein the toxic substance is benzo [a] pyrene or nicotine. The composition for detoxifying a toxic substance according to claim 1 or 2, wherein the blood disease marker is reduced. The composition of claim 5, wherein the blood disease indicator is creatinine, bilirubin, or glucose. The composition for detoxifying toxic substances according to claim 1 or 2, wherein the composition is a food composition. 8. The composition of claim 7, wherein the food composition is selected from the group consisting of beverages, yogurt, tea, ice cream, candy, jelly, bread, rice cake and noodles. The composition for detoxifying a toxic substance according to claim 1 or 2, wherein the composition is administered at a dose of 10 to 5,000 mg / day.