KR20030087726A - Composition for detoxifying toxic substances having Aloe vera gel - Google Patents

Abstract

PURPOSE: A composition containing aloe vera detoxifying toxic material particularly caused by smoking is provided. The composition promotes the excretion of benzo(a)pyrene, nicotine or the like and can detoxify toxic material caused by various pollutants and environmental hormone. CONSTITUTION: A composition for detoxifying toxic material contains aloe vera and reduces a disease indicator in blood such as creatinine, bilirubin or glucose. The composition is a food composition selected from the group consisting of a beverage, tea, ice cream, candy, jelly, bread, rice cake and noodles. It is administered in an amount of 10 to 5,000mg/day.

Description

Composition for detoxifying toxic substances having Aloe vera gel}

The present invention relates to a composition for detoxifying a toxic substance including aloe vera gel, and more particularly to a composition for detoxifying a toxic substance caused by smoking.

The biggest problem in modern society is the disease caused by smoking toxic substances. For example, various diseases caused by tobacco are caused by more than 4,000 harmful toxins (including carcinogens). In recent years, smoking has threatened the health of not only direct smokers but also secondhand smokers, so smoking is a problem for all of us. Smoking accounts for 30% of the total population dying from cancer, with 85% of male lung cancer deaths and 80% of female lung cancer deaths. Cancers caused by tobacco are diverse, including cervical cancer and leukemia in women. In addition, it causes gastrointestinal disease, heart disease, stroke and atherosclerosis. Therefore, when the detoxification by inhibiting the action of tobacco toxic substances or by promoting the excretion can be prevented in advance a number of diseases caused by smoking.

Benzo [a] pyrene (BaP), a representative toxic substance caused by smoking, is a harmful carcinogen, and nicotine is not only a precursor for producing tobacco-specific carcinogens such as NNK, but also the habit of tobacco And various toxic effects.

Benzo [a] pyrene, a toxic substance of tobacco, is also a pollutant contained in soot or automobile exhaust. With the development of the industrial society, environmental pollution due to the concentration of the population is increasing day by day, and the pollutants around the incinerators and the exhaust emissions from automobiles, industrial complexes, etc. are exacerbating environmental conditions through various forms and routes. . Among the endocrine obstructions (aka environmental hormones), which are emerging recently, a lot of pollutants are derived from the environment, and some of them may cause problems in the human endocrine system. Automobile exhaust is also a collection of carcinogens, and the gas produced by burning fuel contains a number of toxic benzo [a] pyrenes, which are particularly representative of toxic substances. It is known to make a substance. Even small doses of benzo [a] pyrene are always at high risk for lung cancer, liver cancer, stomach cancer, bladder cancer, esophageal cancer and skin cancer.

In addition, the toxic substance increases blood levels of creatinine, glucose, and bilirubin, which are factors related to the disease of blood, and the increased concentration of the factors causes disease in the human body.

Creatinine is a waste product produced by the breakdown of creatine after protein is used as an energy source in muscles. It is discharged into the blood and then filtered out of the kidneys. Creatine and creatinine are filtered out of the glomeruli, but creatine is completely reabsorbed in the tubules, whereas creatinine is not resorbed in the tubules and is secreted from the tubules when blood levels are high. For this reason, blood creatine concentration is considered clinically important as a measure of renal function. In recent years, many diabetes mellitus in adults is mainly insulin-independent diabetes. Appears and is determined by measuring the level of glucose in the blood. In the case of bilirubin (bilirubin), in the case of prehepatic jaundice or hepatic jaundice, blood concentration increases.

Aloe is a medicinal plant belonging to the family Liliaceae and has been widely used in folklore since ancient times because of its various effects. The civilian has long used aloe as a suntan ointment, suntan powder, cosmetic cream, lotion and shampoo. However, scientific researches on the exact effects and mechanisms of action of aloe, which have been used for a long time, have been made relatively recently. Recent studies on aloe have been reported to show a variety of activities such as wound healing, anti-inflammatory activity, ulcer treatment activity, anticancer activity, antimicrobial activity, immunomodulatory activity, cell growth promoting activity.

As a result of diligent research on aloe, the present inventors have found for the first time that aloe vera gel has a detoxifying effect of toxic substances, particularly toxic substances caused by smoking or automobile exhaust, such as benzo [a] pyrene. The invention has been completed.

It is an object of the present invention to provide a composition for detoxifying a toxic substance comprising aloe vera gel.

1 is a graph showing the excretion effect of benzo [a] pyrene on in vivo ( in vivo ) for aloe vera gel administration,

Figure 2 is a graph showing the excretory effect of cotinine in vivo on aloe vera gel administration,

Figure 3 is a graph showing the blood concentration of creatinine in vivo for aloe vera gel administration,

Figure 4 is a graph showing the blood concentration of in vivo compensatory glucose for aloe vera gel administration,

Figure 5 is a graph showing the blood concentration of bilirubin in vivo for administration of aloe vera gel,

The present invention provides a composition for detoxifying a toxic substance including aloe vera gel.

In particular, the present invention provides a composition for detoxifying a toxic substance which can be detoxified due to smoking, and the toxic substance is, for example, benzo [a] pyrene, nicotine, tar and the like.

The present invention also provides a composition for detoxifying a toxic substance, which reduces blood disease markers. The blood disease indicator is creatinine, bilirubin or glucose and the like.

The present invention also provides a food composition having a detoxifying effect including aloe vera gel.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

A first aspect of the present invention is to provide a composition for detoxifying a toxic substance comprising aloe vera gel.

The aloe vera gel included in the composition of the present invention is preferably a gel obtained from aloe vera alone, or powdered aloe vera gel, or a mixture of aloe vera gel and powder in a suitable ratio. Can be used. In addition, a commercially available aloe product containing a large amount of the high physiological activity effect of aloe may be used, and in particular, active aloe ^TM (Aloecorp, USA) and the like may be used. The active aloe ^TM is a product containing more of the middle range of polysaccharides with the highest bioactive activity of aloe than aloe vera, and is 200 times concentrated powder of aloe vera gel.

The composition of the present invention may contain only aloe vera gel, but may include a pharmaceutically acceptable carrier or the like for ease of administration.

The compositions of the present invention may be formulated by methods known in the pharmaceutical art, and may be mixed with themselves or with a pharmaceutically acceptable carrier, excipient, and the like to be a pharmaceutically conventionally acceptable pharmaceutical agent such as a liquid or a syrup. , Capsules and the like, which can be administered orally or parenterally. The composition of the present invention is preferably administered orally as a powder.

The liquid, capsules and the like containing the composition of the present invention is preferably used as a health functional food, the term "health functional food" used in the present invention is used as a raw material or ingredient having a useful functionality to the human body Refers to food products manufactured and processed in the form of tablets, capsules, powders, granules, liquids, and pills.

The composition of the present invention is appropriately selected according to the absorption of the active ingredient in the body, the rate of excretion, the age and weight of the patient, the sex and condition, the severity of the disease to be treated, etc., but generally in adults 10 to 5,000mg / day, It is preferable to administer at 100 to 2,000 mg / day. The unit dosage form so formulated may be administered several times at regular time intervals as needed.

Hereinafter, the present invention will be described for a smoker who is exposed to the most harmful toxic substance to the human body, preventing, inhibiting, promoting the excretion or detoxification of toxic toxic substances in aloe, but exemplified benzo [a] Since pyrene is present not only in cigarette smoke but also in automobile exhaust, etc., the composition for detoxifying the toxic substance of the present invention is not limited to the detoxification of toxic substances due to smoking.

As described above, benzo [a] pyrene, which is a representative toxic substance caused by smoking, is a harmful carcinogen, and nicotine, another toxic substance, is not only a precursor for producing tobacco-specific carcinogens such as NNK, but also habits of tobacco and It has a variety of toxic effects.

The composition of the present invention exhibits detoxification by promoting the excretion of toxic substances such as benzo [a] pyrene and nicotine.

A second aspect of the present invention is to provide a composition for detoxifying a toxic substance, which reduces blood disease markers.

The composition of the present invention showed an excellent effect even in the study of biochemical indicators of factors related to the disease of the blood of the following experimental example. Thus, the composition of the present invention by lowering the blood concentration of factors associated with the disease of the blood, ie creatinine, glucose, bilirubin as described above to normalize the abnormal increase caused by the disease or disorder caused by the increase of these factors It can be expected to treat and prevent diseases.

The third aspect of the present invention is to provide a food composition having a toxic substance detoxifying effect including aloe vera gel.

The food composition is preferably a beverage, yogurt, tea, ice cream, candy, jelly, bread, rice cake or noodles, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to the following examples. These examples are for illustrative purposes of the present invention and are not intended to limit the protection scope of the present invention.

Experimental Example 1. Measurement of excretion of benzo [a] pyrene

For 10 smokers who smoke one or more packs of cigarettes daily, oral administration of commercially active Aloe ^TM (Aloecorp, USA) twice daily (morning and evening) at a dose of 600 mg / day for 1 month, and 1 week Urine was collected in the morning (8-9 hours) at intervals. Urine was collected in the same manner as above, with 10 smokers who smoked one or more packs of cigarettes per day that were not administered the active aloe as positive controls and 10 non-smokers as negative controls.

To 4 ml urine sample 150 μl (200 ng / ml) of internal standard BaP-7,8-dihydrodiol (National Cancer Institute, Bethesda, MD) was added. 4 ml of chloroform was added thereto and shaken for 1 hour. Thereafter, it was centrifuged for 10 minutes (3,500 x g), and 3 ml of the organic phase was taken and dried with N ₂ gas. After dissolving in 300 μl methanol and filtering with a 0.45um filter to prepare a sample.

The sample (20 μl) was quantified benzo [a] pyrene using an HPLC system. HPLC analysis conditions are as follows.

1.HITACHI-Model L-7200 Autosampler; 2.Model L-7100 Pump; 3.Model L-7610 Degasser; 4.Model D-7000 / 6000 Interfadce Module; 5. Model FP-920 Fluorescence Detector (JASCO); 6. colume, Novapak C18 column (15 × 0.37 (i.d.)), 4um paricle, waters; 7. Solvent gradient 0 min-A solvent (80%), B solvent (20%), 20 min-A solvent (12%), B solvent (88%) (linear gradient) (A solvent-50: 50 (water : Methanol) / B solvent-methanol); 8. Spill rate-1.2 ml / min.

The amount of BaP was calculated by the height ratio by Fluorescence Detector (exi / emi: 265 / 450nm), and the unit was expressed in ng / ml, and the results are shown in FIG. 1. Statistical analysis examined the statistical significance of Bonferroni t-test and Dunnett's method of one-way analysis of variance (ANOVA) (*: p <0.05, **: p <0.01, ***: p <0.001).

As can be seen in Figure 1, the aloe vera gel administration group of the present invention was significantly (p <0.001) benzo [a] pyrene excretion was promoted from the second week after aloe vera gel administration compared to the control group, 3 The excretion of benzo [a] pyrene was also accelerated significantly at week 4 (p <0.001).

Experimental Example 2. Measurement of excretion of cotinine

As in Experiment 1, two groups of smokers were administered with active Aloe ^TM (Aloecorp, USA) (600 mg / day) for one month, and urine in the morning (8-9 hours) at weekly intervals. ) Was collected.

2 ml NaOH (5 mol / L) was added to a 4 ml urine sample and 100 μl of 2-phenylimidazole (20 μg / ml. Stig. Louis, Mo.) was added as an internal standard. 4 ml of chloroform was added thereto and shaken for 1 hour. Thereafter, it was centrifuged for 10 minutes (3,500 x g), and 3 ml of the organic phase was taken and dried with N ₂ gas. After dissolving in 300 μl methanol and filtering with a 0.45um filter to prepare a sample.

Cotinine was quantified in the sample (20 μl) using the same HPLC system as in Experimental Example 1, and the results are shown in FIG. 2.

As can be seen in Figure 2, the aloe vera gel administration group of the present invention was significantly (p <0.001) promoted the excretion of cotinine from the third week after aloe vera gel administration compared to the smoking control, even at 4 weeks after administration Significantly (p <0.001) the excretion of cotinine was promoted.

In this way, aloe vera gel promotes the excretion of nicotine, a metabolite of nicotine, and thus, promotes the excretion of nicotine, a poisoning component of tobacco.

Experimental Example 3 Measurement of Blood Levels of Creatinine, Glucose and Bilirubin

As in Experiment 1, two groups of smokers were administered with active Aloe ^TM (Aloecorp, USA) (600 mg / day) for one month, and blood was collected in the morning (8-9 hours) at weekly intervals. It was. The measurement of blood levels of creatinine, glucose and bilirubin is as follows.

(1) How to measure the concentration of creatinine

Blood was collected from the experimental group, centrifuged (3,000 rpm, 15 minutes), and the supernatant (serum) was separated. 15ul serum was injected and analyzed using Autoanalyzer Hitachi 7170. Jaffe Kinetic method was used for the analysis.

(2) measuring the concentration of glucose

Blood was collected from the experimental group, centrifuged (3,000 rpm, 15 minutes), and the supernatant (serum) was separated. 2.5ul of serum was injected and analyzed using Autoanalyzer Hitachi 7170. Assay, oxidase method was used.

(3) method for measuring the concentration of bilirubin

Blood was collected from the experimental group, centrifuged (3,000 rpm, 15 minutes), and the supernatant (serum) was separated. 4ul of serum was injected and analyzed using Autoanalyzer Hitachi 7170. Jendrassick-Cleghorn method was used for the analysis.

Plasma concentrations of creatinine, glucose and bilirubin of the sample (20 μl) were measured and compared with the control group, and the results are shown in FIGS. 3, 4 and 5.

As shown in FIG. 3, the aloe vera gel-administered group of the present invention significantly decreased (p <0.01) the blood creatinine concentration at 3 weeks after the aloe vera gel administration compared to the control group, and also significantly at 4 weeks ( p <0.001) decreased.

In addition, as shown in Figure 4, the aloe vera gel administration group of the present invention significantly decreased (p <0.001) over 2, 3, 4 weeks after aloe vera gel administration compared to the smoking control.

In addition, as shown in Figure 5, the aloe vera gel administration group of the present invention significantly decreased (p <0.05) blood bilirubin concentration from the first week after the aloe vera gel administration compared to the smoking control group, 2, 3, 4 weeks Also decreased significantly (p <0.001).

3, 4, and 5, the administration of aloe vera gel lowers the blood concentration of all the factors related to the disease of the blood, it can also be expected to treat and prevent the disease caused by the increase of the factors.

Next, the present invention will be described in more detail by way of examples.

Example 1 Preparation of Capsule

Active Aloe ^TM 600mg

Lactose 50mg

Starch 50mg

Talc 2mg

Magnesium stearate appropriate amount

The capsules were prepared by mixing the above ingredients and filling gelatin capsules according to a conventional method for preparing capsules.

Example 2 Preparation of Capsules

Aloe Vera Gel Extract 200 mg

Lactose 100mg

Starch 93mg

Talc 2mg

Magnesium stearate proper amount

The capsules were prepared by mixing the above ingredients and filling gelatin capsules according to a conventional method for preparing capsules. The aloe vera gel extract was obtained by extracting the aloe vera in a conventional manner.

Example 3 Preparation of Liquid

Active Aloe ^TM 600mg

20 g of sugar

20 g of isomerized sugar

Lemon flavor

Add 100 ml of purified water

The above components were mixed according to a conventional method for preparing a liquid, and filled into 100 ml brown bottles and sterilized to prepare a liquid.

Example 4 Preparation of Liquid

Aloe Vera Gel Extract 1,200 mg

20 g of sugar

20 g of isomerized sugar

Lemon flavor

Add 100 ml of purified water

The above components were mixed according to a conventional method for preparing a liquid, and filled into 100 ml brown bottles and sterilized to prepare a liquid. As in Example 2, the aloe vera gel extract was obtained by extracting the aloe vera in a conventional manner.

Example 5 Preparation of Beverages

To prepare a composition comprising a general functional beverage base comprising 5% by weight active aloe ^TM and 0.05% food coloring, 0.05% orange essence, 7.0% fructose, 0.1% citric acid, 0.05% vitamin C, Purified water was added to prepare a beverage.

Example 6: Preparation of Yogurt

1% by weight of the active aloe ^TM of the present invention was added to a common skimmed milk powder (Seoul Milk Products), followed by heat treatment at 80 ° C. for 30 minutes. After cooling to 37 ℃ yogurt strain inoculated with Lactobacillus Bulgaricus, fermented in a 37 ℃ thermostat for 12 hours, and then refrigerated at 4 ℃ to prepare the yogurt.

As can be seen from the above, the composition for detoxifying a toxic substance comprising the aloe vera gel of the present invention has an excellent toxic substance detoxifying effect by promoting the excretion of toxic substances such as benzo [a] pyrene, nicotine, and blood diseases. Biochemical indicators of related factors also show superior effects, which can detoxify toxic substances caused by various pollutions in modern society, environmental hormones and smoking.

Claims (8)

Composition for detoxifying toxic substances, including aloe vera gel. The composition of claim 1, wherein the toxic substance is generated by smoking. The composition of claim 1, wherein the toxic substance is benzo [a] pyrene or nicotine. According to claim 1, Toxic substance detox composition, characterized in that for reducing the disease marker in the blood. The composition of claim 4, wherein the blood disease indicator is creatinine, bilirubin, or glucose. The composition for detoxifying toxic substances according to claim 1 or 2, wherein the composition is a food composition. The composition of claim 6, wherein the food composition is selected from the group consisting of beverages, yogurt, tea, ice cream, candy, jelly, bread, rice cake and noodles. The composition of claim 1 or 2, wherein the composition is administered at a dose of 10 to 5,000 mg / day.