KR100406158B1 - The human body a poisonous toxic substance eliminate waste matter from the system natural product a manufacturing process - Google Patents

Abstract

The present invention relates to a method of manufacturing a herbal extract to remove toxic components in the human body, and more particularly, by mixing at least six or more selected from among natural medicinal herbs such as wild buckwheat, algae grass, young ginseng, honeysuckle, clear tea, licorice, and cedar. By preventing the harmful effects of tobacco smoke and to remove the toxic components in the human body caused by tobacco, the present invention relates to a method for producing an herbal extract excellent.

Description

The human body a poisonous toxic substance eliminate waste matter from the system natural product a manufacturing process}

The present invention relates to a functional food with an excellent removal effect to remove harmful components in the human body, and more specifically, natural herbal medicines of wild buckwheat, algae grass, saengsam, honeysuckle, clear tea, licorice, marshmallow and the like as a main ingredient manufactured by strengthening and combining them The present invention relates to a herbal extract for removing toxic components in the human body and a method of manufacturing the same.

There are many substances and elements that harm people's health, but the most damaging is tobacco. Tobacco is the largest preventable cause of death in our time. The research on the relationship between smoking and diseases has been intensively studied since the mid-1900s, and there is a continuous evidence of increasing incidences of various diseases including lung cancer caused by smoking and other health problems. In fact, about 4,000 species were found in cigarette smoke as early as present, and it is estimated that there are more than 100,000 kinds of components in mainstream smoke.

About 4000 of these components account for more than 95% of the total mainstream smoke, and thousands of other components are present in extremely small amounts. Tobacco smoke is caused by the incomplete combustion of tobacco, and if completely burned, only water and carbon dioxide are produced. Incomplete combustion is caused by incomplete combustibility of some tobacco leaf components, insufficient oxygen supply and temperature difference when the cigarette burns, and in addition, it occurs through the process of distillation, sublimation, pyrolysis, thermal synthesis, and volatility when tobacco is burned.

Tobacco smoke is an aerosol, a mixture of liquid or fine particles in a gas that passes through a Cambridge glass fiber filter (the efficiency of filtering more than 99% of particles larger than 0.1 μm in diameter). Or vapor component, consisting of nitrogen (59%), oxygen (13.4%), carbon dioxide (13.5%), carbon monoxide (3.2%), aldehydes, ketones, Low molecular weight volatile components such as alcohols and esters are included. The material filtered by the Cambridge glass fiber filter is called Particulates phasecomponents, which accounts for about 8% of tobacco smoke, and there are at least 38 known carcinogens, including carcinogens, and carcinogens, and nicotine. And organic chemicals.

According to a recent study, cigarettes are expected to remain the biggest risk factor for human health in the 21st century, and to grow in size.

Nevertheless, it is not easy for a tobacco lover to quit smoking habits, and there is no easy means to eliminate various diseases caused by smoking while continuing to smoke.

The inventors have conceived whether foods cannot be used to prevent the harmful effects of tobacco smoke and to remove toxic components in the human body due to tobacco. The reason is that the consumption of food does not change the smoking habit, so it is easy for smokers to carry out.

As a result of studying various combinations of herbal medicines, the inventors have found a special combination that is effective against toxic in the human body and have completed the present invention.

Another object of the present invention is to extract sufficiently by adding an aqueous solvent to each dry powder and extract or extracts containing the buckwheat, dendritic grass, young ginseng, honeysuckle, clear tea, licorice, marshmallow, and the extract is separated from the solid residue, concentrated and dried It is to provide a method for producing a functional food for removing the detoxifying components in the human body to obtain a solid.

It is to provide a functional food for removing toxic components in the human body consisting of the following composition.

(1) buckwheat, (2) green grass, (3) fresh ginseng, (4) honeysuckle, (5) green tea, (6) licorice (road), (7)

Houttuynia cordata Thunb (Polypara cordata Bueck) of ingredient (1) is also called medicinal herb, juice, fish vinegar, doctrine, and three hundred vines.

The main component is methylnonylkenone CH ₃ (CH ₂ ) ₈ COCH ₃ (oxidation product of decanoyl acetaldehyde) myrcene C ₁₀ H ₁₆ , laurin aldehyde C ₁₁ H ₂₃ CHO, caprinaldehyde C ₉ H ₁₉ CHO, capr It is an acid, and there are many other ingredients, and the leaves contain urea citrate and 2.7% of inorganic compounds released in water.

Cuercitrin has urinary action (100,000-fold concentration) and cardiac action, and it has antibacterial action against E. coli, typhoid bacteria, paratyphoid bacteria, bacillus, gonococci, Staphylococcus aureus and capillary potentiation.

Decanoyl acetaldehyde has antimicrobial activity against non-pathogenic fungi, as well as ringworm and athlete's foot fungi, as well as fungi against staphylococcus aureus, gonococcus and anti-acid bacteria. Antimicrobial activity against staphylococcus is 1: 40,000 and is stronger than sulfa.

Its application is an inflammatory drug, a diuretic poisoning drug, gonorrhea, urethritis, cystitis, uterus, pneumonia, bronchitis, bite, athlete's foot, antidepressant, ulcer, wind and sinusitis.

As a private prescription, juice is produced, and it is attached when athlete's foot, athlete's foot, hemorrhoids, snake venom, and lacquer rise. Leaves and stems are used for swelling and peeing, and the roots are used for wind poison and swelling.

Geranium nepalense Sweet (G. thunbergii Siebold et Zuccarini) of component (2) is another name, also called hyacinth, using the stem and leaves of the plant.

The main components of the outpost are pyrogalgaltanin, glutinous acid, succinic acid, quercetin and its glycosides, and camphorol and camphortrin C ₂₇ H ₃₀ O ₁₄ 3 1 / 2H ₂ O (dhamnoside of camphorol).

According to other sources, tannins, which are based on geranin, a type of ellagtanin, have been identified, and protocatechinic acid, pyrogallol, ellagic acid, brevifoline, and corylazine have been identified in the outpost.

Tannin content is 4.7% in outpost, 20.3% in leaves, 3.8% in stems and 4.6% in roots. The content in leaves is highest in June and August and the lowest in winter.

The main action is the water extract or alcohol extract of the outpost to increase the tension of the intestine. Diarrhea is as pronounced as bismuth preparations, and large amounts do not have side effects and do not reduce the taste of rice.

E. coli, typhoid bacteria, E. coli bactericidal action.

Cuscuta japonica Choisy of ingredient (3) is picked with seeds and dried in the sun.

Seeds contain about 40mg% of resin-like glycosides, amylase, and provitamin A, sugar, and camphorol in the outpost.

It is used as a gangjeong tonic in the treatment of motion, and is used for stony, oil well, and back pain, and it is also used as a sedative, pain medicine, and diarrhea.

In folk medicine, it is used to wash the face to remove papules (pulpulum), and also to use it as a sputum medicine, blood poisoning medicine, and also as a sweat medicine, old wind medicine, hot water medicine, sputum medicine, insect killing medicine and diarrhea medicine. It is also effective in treating kidney disease and high blood pressure.

Lonicera japonica Thunb of ingredient (4) is also called gold and silver, mistletoe, and uses the stem and leaves of the plant.

The components include luteolin C ₁₅ H ₁₀ O ₆ (5,7,3,4-tetrahydroxyflavones) and inositol C ₆ H ₁₂ O _6. The flowers are inositol and lead-like substances (ceryl alcohol, sterol) , Arachnic acid, myristic acid, ellidinic acid, linoleic acid, linoleic acid).

The leaves and stems contain 5-8% tannins, and the leaves contain loganine, about 19% nitrogen-containing substances, and roniserine C ₂₇ H ₃₀ O ₁₅ 2H ₂ O, luteolin-7-rhamnoglucoside. .

Iridoid compounds roniseiroid and sweroside were isolated from the leaves of the same genus L. morronii.

As a function, flower decoction has the effect of urinating and increasing blood sugar, and also has a bactericidal effect on red bacteria, typhoid bacteria, staphylococci, and pneumococci. There is also a protective effect of peptic gastric ulcer, sympathetic nerve excitement, smooth muscle paralysis.

It is used in motion therapy when it is opened in early stages of wind, with flowers, leaves, stems, fever medicines, poisonous medicines, urine medicines, and sperm medicines. Many different types of washing are washed away. It burns like charcoal and is used by medicine.

Private prescription is used to treat cancer. It is good for stomach cancer if you eat it like water for a long time. In particular, it is washed or tingled with endometritis, lymphadenopathy fistula and ophthalmitis.

Cassia obtusifolia L. is another name called red bean, and it uses the seed of the plant.

Seeds cut off the stems when the fruit is black in autumn, and then beat them together to collect seeds. The weight of 100 is 1.7 ~ 2.7g. It means to brighten the eyes, and the plant of its name also contains C.Tora L., which grows in tropical Asia. This plant is not suitable for Korean climate, it does not bear fruit, and the end of the leaf is concave. The seeds of 100 seeds are small (1.6-2.0g) and are not glossy.

In addition to the name of the covenant, the name of the covenant is the name of the first and the last name. There is literature that treats the same name as the same covenant, but it is the nickname of Mr. Wild Mandrami and the name of stone is the shell of raw abalone. It is said that all these medicines, which have the name "Blank", are bright in the treatment of motion.

As a component, emodine, obtufofolin, obbutusin C ₁₈ H ₁₆ O ₇ , chrysoobtucin C ₁₉ H ₁₈ O ₇ , and aurantioobtucin C ₁₇ H ₁₄ O ₇ and its glycosides were separated. TL C confirmed chrysopanol, chrysopanol antrone, aloe emodine, and lanes.

Napropyron derivatives and glycosides thereof, such as lubrofusarin, nor- lubrofusarin, lubrofusaringenthiobioside, isocoumarin intorallactone, and yellow pigment, toracrison, were isolated.

The leaves contain camphorol-3-diglucoside C ₂₇ H ₃₀ O ₁₆ 2H ₂ O.

As a function, the water or alcohol extract of the seed has a blood pressure-lowering effect in animal experiments.

The application is God's treatment in motion, lowers the liver heat, and the wind and heat lowering effect is said to be a pain due to the wind, it is used to treat eye diseases.

As a private prescription, it is often mixed with a weak diarrhea tonic medicine and drunk like a tea. Lubricate the intestines so that the stools are good and lower blood pressure.

The licorice (Glycyrrhiza glabra L.) of ingredient (6) is also known as chrysanthemum, straight licorice, sensational liquor and uses plant roots.

Roots and root stems are carved out in the autumn, trim the stems, rinse in water and dry in the sun.

The rhizome and root glycyrazine content is higher in autumn than in summer. The glycyrazine content of biennial licorice is 3.8% in April, 3.7% in May, 3.3% in July, 3.8% in August, 5% in September and 6% in October.

The content of glycyrrhizin along the roots is 6.91% for the root of the biennial licorice and 8.04% for the thin root. Therefore, it is reasonable to use not only the root and root stem as medicine, but also the root. Also, the skin (13.06%) is higher than the neck and water (10.42%). When peeling, only the corked skin should be peeled off, so that the skin is not damaged. Roots and stems contain 5-14% glycyrrhizin (potassium or calcium salt of glycyrrhizin acid) (23% more) and glabraic acid (glycitretin) C ₃₀ H ₄₆ O ₅ .

Glycilisic acid C ₄₂ H ₆₂ O ₁₆ is triterpenoids saponin, melting point 205 ℃, decomposition point 220 ℃, [d] _D ^{20 +} 58.5 °, well soluble in alcohol and hot water, not soluble in ether. After cooling in hot water, it hardens like grass. Decomposition of water gives tasteless glycyrrhetinic acid C ₃₀ H ₄₆ O ₄ and two molecules of glucuronic acid C ₆ H ₁₀ O ₇ . Glycyretinic acid is a β-amirin-based triterpenoid, which has two kinds of α and β substances, which are easily changed. But its esters do not change from one another. α-Glycyretinic acid is a plate-like crystal (dilute alcohol), melting point 283 ° C., [α] _D ^{20 +} 140 ° (ethanol), soluble in ethanol, dioxane and chloroform. β-Glycyretinic acid is needle crystal (ethanol), melting point 296 ~ 300 ℃ [alpha] _D ²⁰ +86。 (alcohol), soluble in ethanol, pyridine, acetic acid and not soluble in petroleum ether.

In addition, flavono glycosides, liqueurin C ₂₁ H ₂₂ O ₉ , neoriquiritin, and lamnoricuritin (these non-sugar parts are all liqueurinine) and the corresponding galcon glycosides called isoricuitin and neo Isoricuiritin, isoram norikuiritin, and liqueur seed (the non-sugar part of these are all Isorikuuritingenin). Rhamnoricuritin is also called liqueuritoside.

More than 27 flavonoid analogs were identified by chromatograph in licorice roots.

Β-sitosterol, formmononetin, ricolycidine, lycoricone, and ricore-oligonane were identified in the roots of curved licorice. In addition, dry leaves and stems when flowering can be used as house animal feed with 14-22% protein, 2-5% oil, 36-62% nitrogen-free extract, and 5-20% ash.

Flavonoid content in licorice changes with the season of growth. It is the highest when the roots start to fall, and the leaves and stems have fruit. In the fall, the content of liqueur seeds is about 0.5%. Licuraside accounts for about 10% of the total flavonoids.

Stems and leaves include saponaretin, glyphoside and bitexin. Saponaretin is about 50% of the total flavonoids and 3% in the outpost when fruit is opened.

Glyceramarin (bitter component of glycyridin) with a bitter taste, 2,4,4-trihydroxygalcon and its glycoside deoxystiggmasterol (melting point 135-136 ° C), β-sitosterol, essential oil (0.03%), Ascorbic acid 11-30mg%, Asparagine (1-4%), Fructose (2.4-6.5%), Glucose (2-4%) Mannitol, Starch (14-30%), Rubber (1.5-4%), Yellow cattle Aspartic acid, oil, resin, tannin, protein, and amino acids include aspartic acid, proline, alanine, threonine, serine, glycine, valine, isoleucine and γ-aminobutyric acid. The most bitter substance is 8%. Among them, 2.5 ~ 3% of the water is released.

Glycirizine, which is known as an active ingredient of licorice root, has a sweet taste of about 40 to 50 times that of candy, and its sweetness can be felt even in a diluted solution of 100,000 times. The water solution foams when shaken, but is weaker than normal saponins and has no or hemolytic action (potassium salt is 1: 400). The glycyrrhetinic acid produced by the hydrolysis of glycyrrhizin is not sweet and hemolytic. Thus, the expectorant action of licorice is presumed to be due to glycyrrhetinic acid. Glysirizine also relieves the respiratory system.

Licorice root extract and glycyrrhizin are non-toxic and relieve poisoning by catcherchloral, strycnin, yohimbine, cocaine, morphine, codeine, atropine and luminal. Releases bacterial and viral toxins such as tetanus toxin, diphtheria toxin and snake venom.

It antagonizes with acetylcholine, histamine, and allergens, and has an adrenaline-like effect.

The poisonous action of glycyrrhizin is related to glucuronic acid, a product of water degradation. Glucuronic acid is a component widely found in animal and plant systems and is combined with toxic substances in the liver to form glucuronide and excreted by urine. Therefore, glucuronic acid is used for drug addiction, alcoholism, food poisoning, jaundice, cirrhosis of the liver, chronic liver, rheumatism, joint pain, nerve pain. The detoxification of glycyrrhizin is not thought to be caused solely by glucuronic acid, but is also related to the action of glycyrazine similar to that of corticosteroids. Glycirizine has the same effect as desoxycorticosterone, the corticosteroid.

Glycyretinic acid reduces the excretion of sodium, chlorine and urine and increases the excretion of potassium in animal experiments.

That is, the ratio of Na / K is lowered. As such, it negatively affects potassium metabolism but positively affects sodium metabolism. It also acts to reduce the ascorbic acid content of the adrenal cortex, which sets conditions to enhance the effects of corticosteroids on water and salt metabolism. This action is also present in licorice extract and glycyrrhizin. The same thing happens when people eat.

Licorice root extract glycyrrhizin is anti-inflammatory. In other words, it has a positive effect on the treatment of experimentally induced formalin arthritis in animals. It inhibits the activity of serum transaminase and increases the activity of adenosine triphosphatase in the liver. The anti-inflammatory action of glycyrrhizin similar to desoxycorticosterone is believed to be due to the degradation product glycyrrhetinic acid.

There is also a view that the corticosteroid-like action of glycyrizine inhibits the reduction of the inactivating Δ ⁴ -3 ketone moiety in vivo.

Licorice root extract has antispasmodic effect similar to papaverine on gastrointestinal smooth muscle in animal experiments. This action is due to the flavonoid component. Licorice flavonoids, which have not been used until now, was developed with known antifungal and anti-inflammatory effects. Licuraside, a flavonoid component, has three times as hard as papaverine and has anti-inflammatory and anti-ulcer activity. And antagonists of acetylcholine and histamine.

Licorice root extract has been known to have a therapeutic effect on peptic ulcer disease in the 1950s. Thus, many experiments have been carried out to find anti-ulcer substances in licorice roots. In the 1960's, flavonoids were obtained and the components were found to work hard. In addition, recent experiments have shown that there are anti-inflammatory and anti-ulcerative effects as well as palliative action.

Licorice has a strong surface activity, so if you eat it with other copper medicines, it helps loosen the ingredients in water to help absorption. It also reduces the toxicity of the medicinal herb and changes the taste.

Licorice root has a slight inhibitory effect against Staphylococcus aureus, Escherichia coli, and Lycobacteria.

When glycyrrhizin is used with anti-cancer drug thiopoxamide, anti-cancer activity reaches 85-95%.

Applications include liquorice sputum cutting, palliative action, anti-inflammatory and anti-inflammatory action, and antihistamine action.

Therefore, sputum acupuncture is used for upper gastrointestinal diseases, because it has a sweet taste, it is also used as a drug in the medicine. It can also be used as a poison or medicine. Nowadays, it is widely used for gastroduodenal ulcers, Edison disease, bronchial asthma, jaundice and hepatitis, eczema and other skin diseases.

Glycyrrhizin is used as a supplement to increase the activity of anticancer drugs.

In motion therapy, the roots are often used for the purpose of mitigating and harmonizing the actions of other medicines in motion. In other words, when used with a hot medicine to relieve heat, and when used with a cold to relieve the cold.

Like palliative medicine or pain 멎 jinjak, sputum pill, cough 멎 yipo, poison pool medicine stomach cramps, stomach pain, sore throat, stomach ulcers, duodenal ulcers and enter into a poisonous prescription.

Rubia (Rubia cordifolia L. var. Mungista Miquel (R. akane Nakai)) of ingredient (7) is also called Cheoncho, Hongcheon, and uses flowers and leaves of plants.

In the root, oxanthraquinone pigment purpurin C ₁₄ H ₈ O ₅ , pseudopurpurin, pseudopurpurin-xylo-glucoside, moongistine, and moongistine-glucoside were separated.

At the roots of R. tinctorum L. and cordifolia L. are alizarin C ₁₄ H ₈ O ₂ and its glycosides, rubitriric acid C ₂₅ H ₂₆ O ₁₃ , galiozin C ₁₅ H ₈ O ₇ , xanthapurpurin C ₁₄ H ₈ O ₄ , furfuroxanthin, rubiandin glycosides, furfuroxanthincarboxylic acid, rubidine and the like. In addition, there are lemon acid, malic acid, grape acid, sugar, pectin. The leaves contain organic acids and trace alkaloids and flavonoids.

Where it works, the root decoction slowly destroys kidney stones and bladder stones. This mechanism of action was initially thought to be due to acidification of urinary acid to dissolve urine in calcium phosphate. Nowadays, chemically similar pigments in the roots dissolve by acting with calcium phosphate to dissolve acidic urine so that the citrate dissolves quickly.

Root preparations do not affect arterial pressure and respiration, but strengthen heart contractions. However, it does not have a clear effect on the heart rhythm. And the pendulum penetrates hard and raises tension. It also has a pissing action and acts as a fungus against cocci. Root preparations lower the tension of the pyelone and bladder smooth muscles and increase the peristaltic contractions of the muscle fibers, making the movement of stones absent. Therefore, the therapeutic effect of this drug is especially good for spasm of urethral smooth muscle.

Root dry extract, root powder, total glycosides and ruberic acid were compared and tested. This formulation urinates better than other formulations and has a more pronounced hardening effect. It also reduces the coagulation time.

Applications include nephropathy, prevention of recurrence after stones and inflammatory phosphate urine. Magnesium phosphate, calcium phosphate stones have good therapeutic effect.

When you take this medicine, the stones become rough, punctured and pink. After 3-4 hours of medicine, the urine turns red. Enough amount should be used to pee the rose.

In motion therapy, it is used when there is no menstruation, endometritis, vomiting or bleeding, and when urine is mixed with blood.

Folk medicine is a pissing medicine with swelling paralysis and fever lowering medicine, especially when there is a fever in the lungs and liver, with phlegm to improve colds, pneumonia, sore throat and blood circulation. It is also used as a pain medicine, Jingyeong medicine, and antiseptic, and is said to be effective against malignant tumors. In addition, anti-inflammatory, estrogen activity, bloody, jingyeong, expectorant, tonic, blood pressure lowering, mercury absorption is said to be effective.

In order to obtain extracts of components (1), (2), (3), (4), (5), (6), and (7), each component may be used simultaneously or separately in an aqueous solvent (e.g., water alone or water). And an excess of a mixture of a hydrophilic organic solvent such as methanol, ethanol, acetone and the like (for example, 5 to 50 times, preferably 10 to 20 times), and the mixture is 100 DEG C, preferably Next, a sufficient time (0.5 to 5 hours, preferably 1 to 4 hours) is extracted at 25 ° C to 100 ° C to remove solids, and the extract is concentrated under reduced pressure (30 to 50 mmHg).

Although the content rate of each said component can be arbitrarily selected over a wide range in principle, it is 5-70% (it represents each weight% above) as solid content.

The mixture containing the above ingredients may be used as the functional food of the present invention as it is, but may be mixed with conventional excipients (lactose, corn starch, microcrystalline, cellulose, magnesium stearate, etc.) as necessary, It can be formulated into a dosage form commonly used in internal medicine such as granules, tablets, capsules and the like. Moreover, it can also be mixed with foods, such as sweets, such as a yoghurt, a candy, a cookie, a jelly, and chewing gum, or a soft drink. Furthermore, it can also be combined with medicine, herbal medicine and the like. In addition, the herbal extract according to the present invention may be prepared in the form of additives of tobacco or may be prepared as a component of the animal feed.

(Example 1)

10 g of 40% ethanol was added to 1 g of the weakly ground dry powder, and extracted at 25 ° C. for 5 hours. After filtering the obtained product, the solid residue was discarded and the extract was concentrated under reduced pressure (30-50 mmHg) at 45-50 degreeC. The solid component obtained was concentrated to a solid content of about 70% and then 0.3 to 0.4 g was obtained as an extract. This product may be used as the functional food of the present invention as it is, or may be combined with other ingredients to be formulated into a pharmaceutical composition or prepared in a food form.

(Example 2)

15 g of purified water was added to 1 g of the dense grass dry powder, and extracted at 90 ° C. for 5 hours. The obtained product was filtered and the solid residue was discarded and the extract was concentrated under reduced pressure (30-50 mmHg) at 45 ° C to 50 ° C. The obtained solid component was concentrated to a solid content of about 70% and then 0.4 to 0.5 g was obtained as an extract. This product may be used as the functional food of the present invention as it is, or may be combined with other ingredients to be formulated into a pharmaceutical composition or prepared in a food form.

(Example 3)

10 g of 40% ethanol was added to 1 g of dried ginseng powder, and primary extraction was performed at 70 ° C. for 5 hours, and then the product was stored firstly, and 40% ethanol was added 7 times and extracted twice at 70 ° C. for 5 hours. The tea product was filtered off and the solid residue was discarded and the extract was concentrated under reduced pressure (30-50 mmHg) at 45 ° C to 50 ° C. The obtained solid component was concentrated to a solid content of about 70% and then 0.4 to 0.5 g was obtained as an extract. This product may be used as the functional food of the present invention as it is, or may be combined with other ingredients to be formulated into a pharmaceutical composition or prepared in a food form.

(Example 4)

15 g of purified water was added to 1 g of dried honeycomb powder and extracted at 90 ° C. for 5 hours. After filtering the obtained product, the solid residue was discarded and the extract was concentrated under reduced pressure (30-50 mmHg) at 45-50 degreeC. The obtained solid component was concentrated to a solid content of about 70% and then 0.4 to 0.5 g was obtained as an extract. This product may be used as the functional food of the present invention as it is, or may be combined with other ingredients to be formulated into a pharmaceutical composition or prepared in a food form.

(Example 5)

10 g of 40% ethanol was added to 1 g of dry powder tea and the product was first extracted at 70 ° C. for 5 hours, and then the product was stored firstly, and 40% ethanol was added 7 times and extracted twice at 70 ° C. for 5 hours. The tea product was filtered off and the solid residue was discarded and the extract was concentrated under reduced pressure (30-50 mmHg) at 45 ° C to 50 ° C. The obtained solid component was concentrated to a solid content of about 70% and then 0.4 to 0.5 g was obtained as an extract. This product may be used as the functional food of the present invention as it is, or may be combined with other ingredients to be formulated into a pharmaceutical composition or prepared in a food form.

(Example 6)

15 g of 40% ethanol was added to 1 g of licorice dry powder, the mixture was extracted at 25 ° C. for 12 hours, the product was filtered off, the solid residue was discarded, and the extract was concentrated under reduced pressure (30-50 mmHg) at 45 ° C. to 50 ° C. The obtained solid component was concentrated to a solid content of about 70% and then 0.35 to 0.4 g was obtained as an extract. This product may be used as the functional food of the present invention as it is, or may be combined with other ingredients to be formulated into a pharmaceutical composition or prepared in a food form.

(Example 7)

15 g of purified water was added to 1 g of dried powder, and the product was first extracted at 90 ° C. for 5 hours, and then the product was stored first, and 7 times of purified water was added again, and extracted twice at 90 ° C. for 5 hours. After that, the solid residue was discarded and the extract was filtered at 45 ° C. to 50 ° C., and then the solid residue was discarded and the extract was concentrated at 45 ° C. to 50 ° C. under reduced pressure (30 to 50 mmHg). The obtained solid component was concentrated to a solid content of about 70% and 0.4-0.5 g was obtained as an extract. This product may be used as the functional food of the present invention as it is, or may be combined with other ingredients to be formulated into a pharmaceutical composition or prepared in a food form.

(Example 8)

Measurement of Nicotine Concentration in Blood

1) Test Animal

ICR Mouse of Wister strain male rats (6 weeks old)

2) Test environment

Animals were housed in 5 wire cages in an animal room set at room temperature 24 ± 1 ° C., humidity 55 ± 5%, ventilation time 12 times / hour, lighting time 12 hours / day (morning time, 8 o'clock, etc.). The feed allowed the free intake of solid feed with water.

3) Administration method

Adapted to the test environment for 2 weeks and animals were tested at 8 weeks of age.

Test substance was orally administered by mixing the present invention with a solid feed.

Mix the invention into 0.5ml / solid solid feed and feed it freely with water

It was administered orally in the body.

4) administration time

Administration was performed at 2 pm and the period was 7 days.

At the end of dosing and at the end of the test, 1 ml of blood was drawn from the heart as a sample for nicotine measurement.

5) Observation Method

Dosing days were observed immediately after administration and in the evening, and the morning and evening thereafter.

Observation was carefully conducted from outside the breeder.

6) Test result of nicotine concentration in blood

Blood was adjusted to pH 9 with dilute ammonia water. Subsequently, the resultant was kept in EXTRELUT COLUMN. (Manufactured by Merck Co., Ltd.) for 15 minutes, eluted with 15 ml of ethyl acetate, and the eluted acetate was distilled off under reduced pressure.

For analysis, use gas chromatography (HP 5890 SERIESП with FID). COLUMN used a glass column 2 m long and 3 mm in diameter filled with 2% Tharmon 1000 + 1% KOH Chromosorb WAM (8 / 100Mesh).

The COLUMN temperature was 100 ° C, the carrier gas was N ₂ , and the flow rate was 60 ml / min.

Table 1. Results after 7 days of administration of the present invention (Nicotine concentration 30mg / kg / 10ml intraperitoneal administration)

Paralysis symptoms Hyperactivity Start Time Hyperactivity End Time Death time Nicotine concentration in blood (㎍ / ㎖) G 1 One 27 " 2'10 " 5'50 " recovery 0.024 2 36 " 2'44 " 6'10 " recovery 0.027 3 43 " 3'25 " 6'51 " recovery 0.015 4 43 " 3'50 " 5'56 " recovery 0.021 5 33 " 3'52 " 6'35 " recovery 0.017 G 2 One 22 " 4'42 " 6'45 " 31'18 " 1.037 2 26 " 4'46 " 6'35 " recovery 0.014 3 20 " 5'40 " 7'25 " recovery 0.012 4 24 " 5'47 " 7'20 " recovery 0.013 5 23 " 4'46 " 6'47 " recovery 0.019 G 3 One 23 " 35 " 56 " 1'33 " 12.178 2 33 " 45 " 1'15 " 1'35 " 12.183 3 29 " 31 " 1'20 " 1'50 " 12.987 4 25 " 54 " 1'35 " 1'57 " 12.969 5 22 " 51 " 1'50 " 2'10 " 11.719 G 4 One 33 " 2'50 " 4'20 " 6'15 " 4.019 2 25 " 2'40 " 4'50 " 7'18 " 3.679 3 24 " 2'45 " 4'47 " 6'45 " 4.095 4 21 " 2'37 " 5'10 " 6'25 " 4.074 5 33 " 2'35 " 4'15 " 6'54 " 4.117

Note: G1: Nicotine administration after pretreatment of the invention.

G2: Pre-treatment of the present invention and administration of the present invention after Nicotine administration.

G3: control Nicotine.

G4: Administration of the invention after administration of the control Nicotine.

The test population was tested five dogs in each group.

7) autopsy

At the end of dosing, there was no gross abnormality in each individual when recovering from necropsy, but when dying, gross abnormalities were found in each individual.

(Example 9)

Urine Nicotine Concentration

1) Test Animal

ICR Mouse of Wister strain male rats (6 weeks old)

2) Test environment

Five animals were housed in a wire cage in an animal room set at room temperature 24 ± 1 ° C., humidity 55 ± 5%, ventilation number 12 times / hour, lighting time 12 hours / day (lighted up in the morning, lighted off at 8 o'clock). Was allowed to consume solid feed freely with water.

3) Administration method

Adapted to the test environment for 2 weeks and animals were tested at 8 weeks of age.

Test substance was orally administered by mixing the present invention with a solid feed.

The present invention was mixed with 0.5 ml / solid solid feed and orally administered to the body as free food with water.

4) Dosing time

Administration was performed at 2 pm and the period was 7 days. At the end of dosing and at the end of the test, 1 ml of blood was collected from the bladder as a sample for nicotine concentration measurement.

5) Observation Method

Dosing days were observed immediately after administration and in the evening, and the morning and evening thereafter.

Observation was carefully conducted from outside the breeder.

6) Test result of nicotine concentration in urine

Urine was adjusted to pH 9 with dilute ammonia water. Then EXTRELUT COLUMN. (Merck Co., Ltd.) was maintained for 15 minutes, eluted with 15 ml of ethyl acetate, the eluted acetate was distilled off under reduced pressure, 100 µl of ethyl acetate was added to the resulting residue to dissolve and used as an assay sample.

For analysis, use gas chromatography (HP 5890 SERIESП with FID).

COLUMN used a glass column 2 m long and 3 mm in diameter filled with 2% Tharmon 1000 + 1% KOH Chromosorb WAM (8 / 100Mesh).

The COLUMN temperature was 100 ° C., the carrier gas was N 2, and the flow rate was 60 ml / min.

Table 2. Results after 7 days of administration of the present invention (Nicotine concentration 30mg / kg / 10ml intraperitoneal administration)

Paralysis symptoms Hyperactivity Start Time Hyperactivity End Time Death time Urine Nicotine Concentration (㎍ / ㎖) G 1 One 22 " 2'20 " 5'30 " recovery 5.524 2 34 " 2'34 " 6'30 " recovery 5.527 3 43 " 3'45 " 6'31 " recovery 5.515 4 46 " 3'40 " 5'36 " recovery 5.531 5 30 " 3'32 " 6'15 " recovery 5.517 G 2 One 23 " 4'32 " 6'35 " recovery 5.537 2 23 " 4'36 " 6'55 " recovery 5.514 3 34 " 5'50 " 7'55 " recovery 5.512 4 21 " 5'17 " 7'40 " recovery 5.513 5 20 " 4'26 " 6'37 " recovery 5.519 G 3 One 20 " 30 " 57 " 1'43 " 0.021 2 33 " 55 " 1'35 " 1'55 " 0.031 3 23 " 41 " 1'50 " 2'30 " 0.037 4 24 " 54 " 1'35 " 1'57 " 0.021 5 25 " 55 " 1'55 " 2'30 " 0.029 G 4 One 31 " 2'30 " 4'10 " 6'45 " 0.219 2 23 " 2'30 " 4'45 " 7'25 " 0.279 3 23 " 2'55 " 4'37 " 6'15 " 0.215 4 23 " 2'47 " 5'50 " 6'55 " 0.214 5 30 " 2'55 " 4'45 " 6'14 " 0.217

(Example 10)

Carbon monoxide concentration in blood

1) Test Animal

ICR Mouse of Wister strain male rats (6 weeks old)

2) Test environment

Animals were housed in 5 wire cages in an animal room set at room temperature 24 ± 1 ° C., humidity 55 ± 5%, ventilation number 12 times / hour, lighting time 12 hours / day (lighted up in the morning, lighted off at 8 o'clock). The feed allowed the free intake of solid feed with water.

3) Administration method

Adapted to the test environment for 2 weeks and tested at 8 weeks of age.

Test substance was orally administered by mixing the present invention with a solid feed.

The present invention was mixed with 0.5 ml / solid solid feed and orally administered to the body as free food with water.

4) C0 expose method

The day after the end of the administration, each animal was housed in an exposure chamber (manufactured by Sugiyamage, Japan), and a mixed gas of 21% O ₂ and 100 ppm CO was introduced into the pump and aspirated for 5 minutes. The concentration in the chapter was examined with a carbon monoxide meter (COM-4, manufactured by Komei Rikagaku Kogyo, Japan).

5) Measurement of CO-Hb Concentration in Blood

Immediately after the end of the exposure, blood was drawn from the heart and 0.5 ml of blood was added to a 5 ml reaction vial. Was used as analytical sample.

For analysis, use gas chromatography (HP 5890 SERIESП, TCD).

COLUMN used a glass column 2m in length and 3mm in diameter filled with molecular sieve 5A (3 / 60Mesh).

The COLUMN temperature was 60 ° C., the carrier gas was H ₂ , and the flow rate was 50 ml / min.

6) Results

The results of measuring the CO concentration in the blood after the test are shown in Table 3.

Table 3. Results after 7 days administration of the present invention

CO-Hb concentration in blood (%) G 1 One 53.8 2 52.9 3 50.3 4 52.8 5 53.5 G 2 One 53.0 2 52.8 3 53.5 4 52.9 5 53.0 G 3 One 69.3 2 70.2 3 69.1 4 68.5 5 68.9 G 4 One 61.5 2 60.8 3 62.9 4 62.1 5 61.8

7) autopsy

At the end of dosing, no gross abnormalities were found in each subject in each group.

(Example 11)

Test for Tar

1) Test Animal

ICR Mouse of Wister strain male rats (6 weeks old)

2) Test environment

Five animals were housed in a wire cage in an animal room set at room temperature 24 ± 1 ° C., humidity 55 ± 5%, ventilation number 12 times / hour, lighting time 12 hours / day (morning time, 8 o'clock at night). Was allowed to consume solid feed freely with water.

3) Administration method

Adapted to the test environment for 2 weeks and tested at 8 weeks of age.

Test substance was orally administered by mixing the present invention with a solid feed.

The present invention was mixed with 0.5 ml / solid solid feed and orally administered to the body as free food with water. Tar was administered intragastrically using a Stomach tube.

4) Observation Method

Dosing days were observed immediately after administration and in the evening, and the morning and evening thereafter. Observation was carefully done from outside the breeder.

5) Results

The results of measuring the tar after the test are shown in Table 4.

Table 4. Result after administration of the present invention (Tar concentration 30mg / kg / 10ml intraperitoneally)

time 1 day 2 days 3 days 4 days 5 days 6 days 7 days G 1 One survival survival survival survival survival survival survival 2 survival survival survival survival survival survival survival 3 survival survival survival survival survival Dead 〃 4 survival survival survival survival survival survival survival 5 survival survival survival survival survival survival survival G 2 One survival survival survival survival survival Dead 〃 2 survival survival Dead 〃 〃 〃 〃 3 survival survival survival survival survival survival survival 4 survival survival survival Dead 〃 〃 〃 5 survival survival survival survival survival Dead 〃

Note: G1: Tar administration after pretreatment by mixing the present invention in a general feed.

G2: Tar administration while the control diet.

The test population was tested five dogs in each group.

As is apparent from the examples, when the functional food of the present invention is administered to the human body and at the same time nicotine is administered, the effect of lowering the amount of nicotine in the blood and the amount of nicotine in the urine is exhibited. In addition, exposure to carbon monoxide lowers the concentration of CO-hemoglobin in the blood. Therefore, the functional food of the present invention reduces the harmful effects of tobacco smoking, and also reduces the amount of nicotine in the blood, thereby preventing various diseases by removing toxic components in the human body caused by tobacco smoking.

Moreover, the functional food of the present invention is consumed as a food or formulated food, so there is no need to interrupt smoking habits, use other items or add extra actions when smoking. Therefore, the effect can be easily achieved without affecting the smoking habit. It is also nontoxic and can be taken without disinfection at all times.

Claims (7)

(A) Houttuynia cordata thunb (poly cordata Bueck), Geranium nepalense Sweet (G. thunbergii Siebold et Zuccarini), Cuscuta japonica Choisy, Lonicera japonica Thunb, Cassia obtusifolia. ), Licorice (Glycyrrhiza glabra L.), step of forming a dry powder selected from the group consisting of Rubia cordifolia L. var. Adding 50 times the amount of an aqueous solvent and heating the mixture at room temperature from 25 ° C. to 100 ° C. for 0.5 to 5 hours to extract the extracted components, and then removing the solids; Concentrating under a reduced pressure of mmHg and then spray drying the method of producing a herbal extract to remove toxic components in the human body caused by smoking, characterized in that it comprises a step of spray drying. (A) Houttuynia cordata thunb (poly cordata Bueck), Geranium nepalense Sweet (G. thunbergii Siebold et Zuccarini), Cuscuta japonica Choisy, Lonicera japonica Thunb, Cassia obtusifolia. ), Mixing licorice (Glycyrrhiza glabra L.), dried powder of Rubia cordifolia L. var. Adding a solvent and heating the mixture at room temperature from 25 ° C. to 100 ° C. for 0.5 to 5 hours to extract the extracted components, and then removing the solids. Concentrating and spray drying the method of producing a herbal extract to remove toxic components in the human body caused by smoking, characterized in that it comprises the step of spray drying. The method according to claim 1 or 2, wherein the herbal extract is prepared in the form of a powder, granule, tablet, or capsule, The method of claim 1 or 2, wherein the herbal extract is prepared in the form of confectionary or a beverage. The method according to claim 1 or 2, wherein the herbal extract is prepared in the form of an additive of tobacco. The method according to claim 1 or 2, wherein the herbal extract is prepared in the form of animal feed. A herbal extract for removing toxic components in the human body caused by smoking produced by the manufacturing method according to claim 1 or 2.