KR20050111785A - 마이크로칩, 핵산 추출용 키트 및 핵산의 추출 방법 - Google Patents

마이크로칩, 핵산 추출용 키트 및 핵산의 추출 방법 Download PDF

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Publication number
KR20050111785A
KR20050111785A KR1020057017815A KR20057017815A KR20050111785A KR 20050111785 A KR20050111785 A KR 20050111785A KR 1020057017815 A KR1020057017815 A KR 1020057017815A KR 20057017815 A KR20057017815 A KR 20057017815A KR 20050111785 A KR20050111785 A KR 20050111785A
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KR
South Korea
Prior art keywords
nucleic acid
microchip
microbeads
flow path
gap portion
Prior art date
Application number
KR1020057017815A
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English (en)
Korean (ko)
Inventor
미찌히로 오오니시
게이스께 모리시마
다께히꼬 기따모리
Original Assignee
소니 가부시끼 가이샤
카나가와 아카데미 오브 사이언스 앤드 테크놀로지
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by 소니 가부시끼 가이샤, 카나가와 아카데미 오브 사이언스 앤드 테크놀로지 filed Critical 소니 가부시끼 가이샤
Publication of KR20050111785A publication Critical patent/KR20050111785A/ko

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00783—Laminate assemblies, i.e. the reactor comprising a stack of plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
KR1020057017815A 2003-03-24 2004-03-24 마이크로칩, 핵산 추출용 키트 및 핵산의 추출 방법 KR20050111785A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2003081605 2003-03-24
JPJP-P-2003-00081605 2003-03-24

Publications (1)

Publication Number Publication Date
KR20050111785A true KR20050111785A (ko) 2005-11-28

Family

ID=33094898

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020057017815A KR20050111785A (ko) 2003-03-24 2004-03-24 마이크로칩, 핵산 추출용 키트 및 핵산의 추출 방법

Country Status (6)

Country Link
US (1) US8012680B2 (US20060234241A1-20061019-C00001.png)
EP (1) EP1607748B1 (US20060234241A1-20061019-C00001.png)
JP (1) JP5162095B2 (US20060234241A1-20061019-C00001.png)
KR (1) KR20050111785A (US20060234241A1-20061019-C00001.png)
CN (1) CN1764842B (US20060234241A1-20061019-C00001.png)
WO (1) WO2004086055A1 (US20060234241A1-20061019-C00001.png)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100945129B1 (ko) * 2008-03-17 2010-03-02 고정문 디앤에이 정제용 칩 및 이를 이용한 정제 방법
KR101428578B1 (ko) * 2012-05-21 2014-08-12 한국과학기술원 미소입자 선택적 포획-회수 장치

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006029798A (ja) * 2004-07-12 2006-02-02 Hitachi Software Eng Co Ltd 試薬を内蔵した高反応効率生体物質検査チップ
US20090149345A1 (en) * 2005-03-07 2009-06-11 Kuraray Co., Ltd. Microchannel array and method for producing the same, and blood measuring method employing it
JP4904973B2 (ja) * 2005-08-08 2012-03-28 ソニー株式会社 核酸回収方法、核酸回収装置、並びに核酸供給装置
JP4657084B2 (ja) * 2005-11-02 2011-03-23 パナソニック株式会社 試料分析用ディスク
JP2007147456A (ja) * 2005-11-28 2007-06-14 Seiko Epson Corp マイクロ流体システム、試料分析装置、及び標的物質の検出または測定方法
US7608399B2 (en) 2006-06-26 2009-10-27 Blood Cell Storage, Inc. Device and method for extraction and analysis of nucleic acids from biological samples
JP4848896B2 (ja) * 2006-08-31 2011-12-28 横河電機株式会社 マイクロチップ
JP4297148B2 (ja) 2006-09-22 2009-07-15 ソニー株式会社 核酸回収装置及び核酸回収方法
WO2009117167A1 (en) 2008-01-02 2009-09-24 Blood Cell Storage, Inc. Devices and processes for nucleic acid extraction
WO2009150583A1 (en) * 2008-06-10 2009-12-17 Koninklijke Philips Electronics N.V. Diagnostic device
KR101563687B1 (ko) 2008-09-02 2015-11-09 삼성전자주식회사 표적 분자 분리를 위한 미세유동 카트리지, 분리장치, 및 이를 이용한 표적 분자 분리 방법
CN103398936A (zh) * 2009-07-07 2013-11-20 索尼公司 微流体装置
WO2012159063A2 (en) 2011-05-19 2012-11-22 Blood Cell Strorage, Inc. Gravity flow fluidic device for nucleic acid extraction
CN108031457B (zh) * 2017-12-22 2020-11-17 深圳逗点生物技术有限公司 一种吸附核酸的复合材料及其制备方法、吸附核酸的装置
IL263582A (en) 2018-12-09 2020-06-30 Vbact Ltd A system for photographing and monitoring flows

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2513608C2 (de) * 1975-03-27 1982-08-05 Degussa Ag, 6000 Frankfurt Verfahren zur Hydrophobierung von Kieselsäuren und Silikaten mit Organosilanen
JPH03220158A (ja) 1990-01-23 1991-09-27 Sumitomo Pharmaceut Co Ltd 光学活性カルバサイクリン誘導体の製造方法
US5726026A (en) 1992-05-01 1998-03-10 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US5587128A (en) * 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
JPH0824600A (ja) * 1994-07-11 1996-01-30 Tokuyama Corp ガラス多孔質膜
US5641400A (en) * 1994-10-19 1997-06-24 Hewlett-Packard Company Use of temperature control devices in miniaturized planar column devices and miniaturized total analysis systems
JP3220158B2 (ja) 1994-11-14 2001-10-22 トラスティーズ・オブ・ザ・ユニバーシティ・オブ・ペンシルベニア 分析対象物質の確定及び処理のためのメソ規模の試料前処理装置及びシステム
KR100306951B1 (ko) * 1995-12-05 2001-11-15 테칸 보스턴, 인코포레이티드 내장된정보과학에의해미세유체공학시스템내의유체유동을구동시키기위해구심가속도를이용하는장치및방법
US20010055812A1 (en) 1995-12-05 2001-12-27 Alec Mian Devices and method for using centripetal acceleration to drive fluid movement in a microfluidics system with on-board informatics
US6193866B1 (en) * 1996-03-27 2001-02-27 Curagen Corporation Separation of charged particles by a spatially and temporally varying electric field
US5900481A (en) * 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
US6270970B1 (en) * 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
US6272939B1 (en) * 1999-10-15 2001-08-14 Applera Corporation System and method for filling a substrate with a liquid sample
WO2001032930A1 (en) 1999-11-04 2001-05-10 California Institute Of Technology Methods and apparatuses for analyzing polynucleotide sequences
CA2290731A1 (en) 1999-11-26 2001-05-26 D. Jed Harrison Apparatus and method for trapping bead based reagents within microfluidic analysis system
SE0001790D0 (sv) * 2000-05-12 2000-05-12 Aamic Ab Hydrophobic barrier
EP2299256A3 (en) * 2000-09-15 2012-10-10 California Institute Of Technology Microfabricated crossflow devices and methods
US6743516B2 (en) * 2000-09-29 2004-06-01 Guardian Industries Corporation Highly durable hydrophobic coatings and methods
US7378280B2 (en) * 2000-11-16 2008-05-27 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
SE0004350D0 (sv) 2000-11-27 2000-11-27 Patrick Griss System och metod för tillförlitliga passiva ventiler och gas volym dosering
US7060171B1 (en) * 2001-07-31 2006-06-13 Caliper Life Sciences, Inc. Methods and systems for reducing background signal in assays
CN1304589C (zh) * 2001-12-07 2007-03-14 清华大学 利用固相载体从生物粒子中分离核酸的方法
US20040011650A1 (en) * 2002-07-22 2004-01-22 Frederic Zenhausern Method and apparatus for manipulating polarizable analytes via dielectrophoresis

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100945129B1 (ko) * 2008-03-17 2010-03-02 고정문 디앤에이 정제용 칩 및 이를 이용한 정제 방법
KR101428578B1 (ko) * 2012-05-21 2014-08-12 한국과학기술원 미소입자 선택적 포획-회수 장치

Also Published As

Publication number Publication date
EP1607748A1 (en) 2005-12-21
CN1764842B (zh) 2012-01-11
US8012680B2 (en) 2011-09-06
JPWO2004086055A1 (ja) 2006-06-29
EP1607748A4 (en) 2012-05-30
CN1764842A (zh) 2006-04-26
EP1607748B1 (en) 2013-05-15
WO2004086055A1 (ja) 2004-10-07
US20060234241A1 (en) 2006-10-19
JP5162095B2 (ja) 2013-03-13

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