KR20050072907A - Composition for alleviation of skin wrinkles comprising herb extract - Google Patents

Abstract

The present invention relates to an antiwrinkle cosmetic composition comprising a herbal extract, the cosmetic composition of the present invention as an active ingredient extract of chrysanthemum, white paper and licorice is a cell activity effect through cell proliferation, collagen synthesis enhancement at the skin cell level Excellent in alleviating wrinkles and fine lines, and does not cause skin side effects or wrinkles.

Description

Wrinkle-relieving cosmetic composition containing herbal extracts {COMPOSITION FOR ALLEVIATION OF SKIN WRINKLES COMPRISING HERB EXTRACT}

The present invention relates to an anti-wrinkle cosmetic composition comprising a herbal extract, and more particularly to an anti-wrinkle cosmetic composition comprising chrysanthemum, white paper and licorice extract excellent in anti-aging effects.

Human skin is constantly undergoing a change, the most representative of which is deterioration of skin function due to aging and reduction of visual beauty. Therefore, one of the areas of interest in cosmetics is how to delay skin aging. The final phenomena that appear in accordance with aging of the skin include wrinkle formation, skin elasticity reduction, aging spot formation, and the like, in particular, wrinkle formation is accepted as the most representative phenomenon.

In general, theories explaining skin aging include mutagenesis of somatic cells, error theory of gene replication, gene program theory, free radical theory, autoimmune response theory, accumulation of toxic substances, and theories of dermal component proteins (M. Rieger, C & T , 110, 94, 1994) There are several such theories involved in skin aging, and free radical theory is known to be a major cause of skin aging.

Free radicals are chemically highly reactive chemicals that occur when the skin is irradiated with UV light or during the respiration of cells in the skin. Most of the free radicals related to aging of the skin are Reactive Oxygen Species (ROS), which include superoxide radical, hydrogen peroxide (H ₂ O ₂ ) and hydroxy radical (Hydroxy radical). ), Singlet oxygen, and the like. These reactive oxygen species generate various active species such as radicals, peroxides, aldehydes, and epoxides, and are the main causes of DNA, RNA, protein, cell membrane and cell structure damage, cancer, tissue damage, and aging. It is thought (Black HS, Photochem photobiol , 46 (2), 213, 1987). However, in spite of many of these studies, it is not yet known how each change is related to the appearance of aging of skin appearance, especially wrinkles, and thus research on the mechanism of wrinkle formation is still ongoing.

Recently, a lot of physiological studies have been made on the causes of skin wrinkles. In order to prevent the formation of wrinkles in cosmetics, methods such as the maintenance of moisture in the stratum corneum by a moisturizer, the protection of the skin from ultraviolet rays or the environment, and the prevention of wrinkles by promoting new cell activity have been applied. Materials used to suppress skin aging so far include enzymes such as superoxide dismutase and catalase, α-tocopherol (vitamin E), and β-carotene (β). Fat-soluble and water-soluble antioxidants or radical scavengers, such as -Carotene, Ascorbic acid (Vitamin C) and Glutathione, have a prophylactic effect of inhibiting wrinkle formation mainly due to skin aging. Meanwhile, vitamin A, alpha hydroxy acid, and the like, which have recently been spotlighted as anti-aging substances, have a healing effect to alleviate wrinkles generated through enhancement of collagen synthesis in the dermis. Vitamin A and alpha hydroxy acids, in particular, have anti-wrinkle effects faster than antioxidants and additional effects such as skin cell regeneration and whitening have been reported (R. Hermitte, C & T , 107, 63, 1992).

However, these antioxidants, radical scavengers, vitamin A, alpha hydroxy acids and the like have a disadvantage in that the stability and safety, etc. when blended in cosmetics.

The present inventors, as part of the study to search for substances having excellent stability and safety at the same time when blended in cosmetics and excellent anti-wrinkle effect, for herbal extracts that are predicted to be aesthetically effective in folk medicine As a result of a detailed investigation of various efficacy and safety, it was found that herbal extracts with excellent anti-wrinkle effect, less peculiar odor and were safe for the skin led to the completion of the present invention.

The herbal extract called "Chrysanthemum Eum" (菊花 飮) has been studied in connection with the present invention has the effect of multiplying the skin by improving the metabolism of the skin in folk medicine, acting on the body's surface layer to release the blockage and heat It disperses the heat of the right face due to the scattering and stress and various pollutions, thereby treating problems such as acne and cremation poison. However, there have been no reports or studies related to the wrinkle relaxation effect by promoting collagen synthesis in chrysanthemum. Thus, the present invention has been completed through the demonstration of the wrinkle relaxation effect.

It is an object of the present invention to provide an anti-wrinkle cosmetic composition comprising an herbal extract with excellent stability and safety and excellent anti-wrinkle effect and less herbal odor when formulated in a cosmetic.

In order to achieve the above object, the present invention provides an anti-wrinkle cosmetic composition comprising the extract of chrysanthemum, white paper and licorice.

Here, the chrysanthemum, white paper and licorice extract is preferably included in an amount of 0.01 to 15.0% by weight based on the whole composition.

In addition, the extract is mixed with chrysanthemum, white paper and licorice in a weight ratio of 0.5 to 1.5: 0.2 to 0.6: 0.05 to 0.2 in distilled water, 1,3-butylene glycol, propylene glycol, ethanol, or a mixed solvent thereof from 5 to 40 wt% can be prepared by extraction with cold or warm needle and filtered.

And the composition of the present invention may be formulated into a cosmetic composition comprising a lotion, essence, lotion, cream or gel.

The present invention provides an anti-wrinkle composition comprising a mixed extract of three herbs of chrysanthemum, white paper and licorice as an active ingredient. As used herein, the anti-wrinkle composition refers to a composition having cell proliferation and collagen synthesis effects.

Looking at the raw herbal medicine of the herbal extract as an active ingredient of the cosmetic composition according to the present invention, Chrysanthemum coccineum flower extract is firstly used as a perennial herb of the dicotyledon plant Campanula, brightening eyes and clearing the head. Drinking chrysanthemum tea is good for cataracts and presbyopia of the elderly. If you put a few dried chrysanthemums in your pillow, you will clear your head and prevent forgetfulness and memory loss. In addition, chrysanthemum is intentional, such as medicinal pneumonia and herbaceous wood, it is written, "If you cure circulatory insufficiency caused by dead skin, bad winds and moist skin, and wear it, your blood will improve, your body will become light, and you won't grow old." . In other words, chrysanthemum in the composition of the present invention is supposed to act to prevent skin aging by improving the metabolism of the skin to make the skin young and glossy, to clean the skin and to regenerate and moisturize the skin.

On the other hand, white paper ( Angelica dahurica root extract) is a herbal medicine made by drying the roots of two-year-old or two-year-old grass of the cotyledonaceae, Apricot plant. It promotes blood circulation and makes the complexion oily and lustrous, ”and it is written that“ relieves blemishes, freckles, and scars on the face. ” In traditional medicine, Bangjapyeonpyeon, it is recorded that white paper is a medicine that has warm properties and removes facial poisons and enhances human metabolism.

In addition, licorice ( Glycyrrhiza glabra root extract) is a perennial herb, which is known to have been poisoned by white medicine, and is added to almost every prescription, and is known to have the effect of harmonizing all medicines. Licorice is also recorded in the herbaceous tree, "the aesthetic sense is warm and harmonizes all medicines. Licorice is effective in various inflammations due to detoxification and clearing. Especially, it has good effects on aging skin, blemish skin, and sensitive skin because it has skin soothing effect, anti-inflammatory effect, skin cell regeneration, and skin metabolism promoting effect.

Hereinafter, the manufacturing method of the cosmetic composition of this invention is demonstrated concretely.

Wrinkle relief cosmetic composition of the present invention is an extract of chrysanthemum, white paper and licorice as an active ingredient. These herbal extracts are prepared by mixing chrysanthemum, white paper and licorice in a weight ratio of 0.5 to 1.5: 0.2 to 0.6: 0.05 to 0.2, followed by distilled water, 1,3-butylene glycol, propylene glycol, ethanol, or a mixed solvent thereof. 5 to 40% by weight, preferably 10 to 25% by weight, followed by extraction with cold or warm needles, followed by filtration with a 0.1-1 μm filter, preferably a 0.2-0.5 μm filter.

The composition of the present invention is prepared by including the herbal extract thus prepared in an amount of preferably 0.01 to 15.0% by weight based on the whole composition. If the herbal extract content is less than 0.01% by weight, it is difficult to expect a substantial anti-inflammatory effect, and if it exceeds 15% by weight, it is not preferable because it is difficult to mask the specific odor of the herbal medicine and adversely affects the skin.

Cosmetic composition of the present invention containing the above herbal extracts may be formulated in preparations such as lotion, essence, lotion, cream or gel.

Hereinafter, the present invention will be described in detail through examples. However, these experimental examples, examples, comparative examples and production examples are only examples of the present invention, and the scope of the present invention is not limited thereto.

Example 1 Preparation of Herbal Extracts

Chrysanthemum, white paper and licorice are mixed in a weight ratio of 0.5 to 1.5: 0.2 to 0.6: 0.05 to 0.2, and extracted by chilling for 30 days in a herbal concentration of 30% by weight in a 7: 3 mixture of distilled water and 1,3-butylene glycol. Filtration was performed with a 0.2 μm filter to prevent the formation of precipitates, followed by 3 days of ultraviolet (UV) light for sterilization to obtain a final herbal extract.

Experimental Example 1: Cytotoxicity Test

In order to verify the primary safety as a raw material used in cosmetics, MTT test was performed by culturing V79-4 cells (Chinese hamster, continuous cell line of lung tissue fibroblasts) on the herbal extract prepared in the above example [Ref. Mossman T. (1983) Rapid colorimetric assay for cellular growth & survival: application to proliferation & cytotoxicity assays. Journal of Immunological Methods 65, 55 ~ 63]. The cytotoxicity test results are shown in Table 1.

sample ^a IC ₅₀ (%, w / v) Sodium Lauryl Sulfate (SLS) 0.005 Squalane 0.05 Herbal extract 5.0

Note) ^a IC _50: inhibitory concentration (Inhibitory Concentration) 50, the concentration that kills 50% of cells

As shown in Table 1, the IC ₅₀ value of the herbal extract was 5.0, which showed cytotoxicity 1000 times lower than that of sodium lauryl sulfate, and cytotoxicity was 100 times lower than that of squalane, which is an excellent safety ingredient. It can be seen that the safety is very good.

Experimental Example 2: Allergy Evaluation (LLNA)

In order to determine whether the herbal extracts according to the present invention are allergenic, an experimental method using acetone: olive oil (4: 1) as a carrier medium was selected. [Kimber I (1990): Identification of contact allergens using the murine local lymph node assay, J. Appl. Toxicol. 10 (3), 173 to 180]. 20%, 40%, and 60% solutions of the herbal extracts were prepared and applied to both ears of the mouse (Balb / c) for 50 days for 3 days to isolate the lymph node (Auricular lymph node) from the rat. Lymph nodes were pulverized into a single cell state and then cultured for 24 hours with the addition of radioisotope (3H-thymidine), and the amplification degree [cpm] of the cells was measured. The results are shown in Table 2.

sample Lymph node cells (10 ⁷ cells / ml) cpm (average) Amplification Acetone: Olive Oil (4: 1) 1.70 2870 Transportation Herbal Extract 20% 1.71 2296 0.8 Herbal Extract 40% 2.04 3157 1.1 Herbal Medicine Extract 60% 3.23 4592 1.6

As shown in Table 2, the herbal extract according to the present invention has an amplification degree (S.I) of 2 or less, showing that there is little possibility of allergy.

Experimental Example 3: Cell Proliferation Effect

Experiments were carried out to measure the cell proliferation effect of the herbal extracts according to the present invention. First, human fibroblast cells were inoculated into 96 well plates (Corning, Inc.), and then cultured. Then, herbal extracts were inoculated at 10%, 5%, 1%, 0.5%, 0.01% and 0.005% concentrations. Was applied for 2 days, and MTT test method was performed to evaluate cell proliferation. Serum 1% was added as a control and serum-free medium was used as a comparative group. The results are shown in Table 3.

Type of sample Cell growth rate after 2 days (%) Control (serum 1%) 161 Herbal Medicine Extract 10% 140 Herbal Medicine Extract 5% 130 Herbal Extract 1% 120 Herbal Medicine Extract 0.5% 110 Herbal extract 0.01% 105 Herbal Medicine Extract 0.005% 102

As shown in the results of Table 3, it can be seen that the herbal extract according to the present invention has a cell activity effect through cell proliferation.

Experimental Example 4: Collagen Synthesis Enhancement Effect

As shown in the results of the above experimental example, the herbal extract of the present invention having excellent cell proliferation was measured as follows.

First, 24-well plates (24-well plate, Corning Inc.) was cultured for 24 hours and then, L-2,3- ³ H-proline (proline), the fibroblast cells (human fibroblast cell) of a person was allowed to react at 24 hours. At this time, herbal extracts were added to the positive control group by concentration, and nothing was treated to the negative control group. After the reaction of L-2,3- ³ H proline, the amount of neonatal collagen synthesized by fibroblasts in the cell medium was measured and compared using collagen degrading enzyme and radioisotope activity analyzer. Serum 1% was added as a control and serum-free medium was used as a comparative group. The results are shown in Table 4.

Type of sample Collagen Synthesis Enhancement Effect (%) Control (serum 1%) 260 Herbal Medicine Extract 10% 200 Herbal Medicine Extract 5% 190 Herbal Extract 1% 169 Herbal Medicine Extract 0.5% 120 Herbal extract 0.01% 109 Herbal Medicine Extract 0.005% 102

As shown in the results of Table 4, the herbal extract according to the present invention can be seen that the synergistic effect of collagen at the cellular level is excellent. However, at a concentration of 5% or more, the collagen synthesis enhancing effect was weak, and at 0.01% or less, it was difficult to recognize the effect.

With the herbal extract of the present invention excellent in cell proliferation effect and collagen synthesis enhancing effect as described above was prepared a preparation as in the following examples.

Examples 2 to 4 and Comparative Example 1

According to the composition of Table 5, Examples 2 to 4 are formulated with a herbal extract in a general manner and Comparative Example 1 is a comparative sample containing no herbal extract, to prepare an oil-in-water emulsion according to the following method: First, the water phase and oil phase After dissolving by heating to 70 to 75 ℃ each, the oil phase was added to the water phase, emulsified for 10 minutes under a homomixer 6000 rpm conditions and cooled to 30 ℃ to prepare an oil-in-water emulsion.

Example 2 Example 3 Example 4 Comparative Example 1 Awards Purified water Remaining amount Remaining amount Remaining amount Remaining amount Triethanolamine 0.15 0.15 0.15 0.15 Carboxy vinyl polymer (1 wt% solution) 15 15 15 15 Dark glycerin 6 6 6 6 Propylene glycol 4 4 4 4 Paraoxybenzoic Acid Ester 0.2 0.2 0.2 0.2 Herbal extract 0.01 1.0 5.0 - Paid Cetostearyl alcohol One One One One Glyceryl Monostearate One One One One Sorbitan monosulfate One One One One POE (20) sorbitan monostearate 1.5 1.5 1.5 1.5 Paraoxybenzoic acid propyl 0.1 0.1 0.1 0.1 Isocetil Myristate 5 5 5 5 Isocetyloctanoate 5 5 5 5 Squalane 5 5 5 5 Spices Quantity Quantity Quantity Quantity

The unit of the numerical value used in Table 5 is weight%. As the carboxyvinyl polymer, Carbopol 941, a product of B.F.Goodrich, USA, was used as a 1% by weight aqueous solution. As monostearate sorbitan, a product sold under the trade name Alascel 60 (ICI) Co., Ltd. was used. Was used.

Experimental Example 5: Fine wrinkles effect

In order to evaluate the anti-wrinkle effect of the products prepared according to Examples 2 to 4 and Comparative Example 1, a sample was uniformly applied to the periphery of the eyes twice a day for 30 women from 40 to 60 years old, The measurement site was defined as crow's feet and carried out for a total of 3 months. The test subjects were divided into three groups, each of which was applied to Examples 2 to 4 containing herbal extracts on the left eye, and the control of Example 1 containing no herbal extracts on the right eye.

After 3 months, the degree of wrinkle reduction was assessed by visual inspection and by measuring the depth of wrinkles by image analysis of the eye wrinkle wrinkles before and after application of the sample. Visual judgment was determined in four stages: no improvement, slight improvement, moderate improvement, and significant improvement compared to each base application control. The visual judgment results are shown in Table 6 by the number of people (persons), and the results of the image analysis method are shown in Table 7 by the depth of wrinkles (μm).

No improvement Minor improvements Moderate improvement A significant improvement commandment) Example 2 5 3 2 0 10 Example 3 4 2 3 One 10 Example 4 2 One 3 4 10

As shown in the results of the fine wrinkles by the visual determination of Table 6, the embodiment according to the present invention shows the alleviation of wrinkles in all cases containing the herbal extracts, especially Example 4 is more than 80% It showed a possible wrinkle relief effect.

Before sample coating (㎛) After application of sample (㎛) Wrinkle depth reduction rate (%) Comparative Example 1 315 308 2.0 Example 2 308 289 6.0 Example 3 290 259 10.8 Example 4 295 241 18.2

As can be seen from the measurement result of the wrinkle depth by the image analysis of Table 7, the depth of the wrinkle is reduced by 2.0% in the case of Comparative Example 1, Examples 2 to 4 is 6.0 to 18.2% depending on the herbal extract concentration It was reduced compared to the control example 1 showed an effective wrinkle depth reduction effect.

As described above, in view of the effect of enhancing collagen synthesis at the skin cell level in Experimental Example 4 and reducing the depth of eye wrinkles in Experimental Example 5, the herbal extract according to the present invention and the cosmetic composition comprising the same are already produced wrinkles It showed an effective relieving effect against, and no skin side effects or wrinkles were observed.

Preparation Example 1: Lotion

The lotion of the present invention was prepared according to a conventional lotion preparation method with the following prescription.

Raw material name Weight% (w / w) Glycerin dipropylene glycol hyaluronic acid polyoxyethylene hardened castor oil polyethylene oleyl ethyl ethanol preservative flavor pigment herbal extract 5.03.00.50.10.15.00.15

Preparation Example 2 Essence

The essence of the present invention was prepared according to the conventional essence preparation method with the following prescription.

Raw material name Weight% (w / w) Cetostearyl alcohol self-emulsifying monostearic acid beeswax squalane isocetyloctanoate dimethyl siloxane monostearic acid sorbitan monostearic acid polyethylene glycol glycerin propylene glycol carboxypolymer triethanolamine preservative flavor pigment freshener extract 1.01.00.55.03.00.30.58.04.00.20.220.25

Preparation Example 3: Lotion

The lotion of the present invention was prepared according to the conventional lotion preparation method with the following prescription.

Raw material name Weight% (w / w) Cetostearyl Alcohol Self-emulsifying Monostearic acid Beeswax Stearic Acid Liquid Paraffin Squalane Alanic Cadmium Oil Isocetyl Octanoate Dimethylsiloxane Monostearic Acid 0.81.00.50.57.05.03.02.00.30.51.24.04.04.00.120.150.25 with appropriate quantity 5.0100

Preparation Example 4 Cream

The cream of the present invention was prepared according to the conventional cream preparation method with the following prescription.

Raw material name Weight% (w / w) Cetostearyl alcohol self-emulsifying monostearic acid lipophilic monostearic acid beeswax liquid paraffin squalane isocetyl octanoate tablet jojoba oil dimethyl siloxane monostearic acid sorbitan monostearic acid polyethylene glycol glycerin propylene glycol beta phosphate gum triethanolamine preservative By adding 3.01.51.50.58.07.04.04.00.31.01.26.04.04.00.060.100.25 with appropriate quantity 7.0100

Preparation Example 5 Gel

The gel of the present invention was prepared according to the conventional gel preparation method with the following prescription.

Raw material name Weight% (w / w) Glycerin Propylene Glycol Ethanol Polyoxyethylene Cured Castor Oil Carboxy Polymer Triethanolamine Preservative Flavor Pigment Extract Extract 4.04.0100.10.300.30

As described above, the cosmetic composition of the present invention comprising the herbal extract is excellent in cell activation effect through cell proliferation, collagen synthesis at the skin cell level, and fine wrinkles reducing effect, deterioration of skin side effects and wrinkles Does not cause.

Claims (4)

Anti-wrinkle cosmetic composition comprising the extract of chrysanthemum, white paper and licorice. According to claim 1, wherein the chrysanthemum, white paper and licorice extract is characterized in that it comprises 0.01 to 15.0% by weight of the total composition. The method of claim 1, wherein the extract is mixed with chrysanthemum, white paper and licorice in a weight ratio of 0.5 to 1.5: 0.2 to 0.6: 0.05 to 0.2, distilled water, 1,3-butylene glycol, propylene glycol, ethanol, or a mixture thereof 5 to 40% by weight of the solvent extracted by cold or hot needle, characterized in that the composition is prepared by filtration. The composition of claim 1 wherein the composition is a lotion, essence, lotion, cream or gel.