KR20050026778A - Cosmetic composition for alleviating skin irritation comprising nanoliposome of intercellular lipids - Google Patents

Abstract

A cosmetic composition for alleviating skin irritation comprising nanoliposome of intercellular lipids is provided, which cosmetic composition induces skin penetration of intercellular lipids, improves barrier function of skin, and reduces water loss from the outer skin. The cosmetic composition for alleviating skin irritation comprises 0.1 to 20.0 wt.% of nanoliposome of intercellular lipids including ceramide, cholesterol and free fatty acid, wherein the nanoliposome is prepared by using a mixture comprising (a) polyol, (b) oily components comprising ceramide and cholesterol, (c) surfactant, (d) phospholipid, (e) free fatty acid and (f) water; the polyol is selected from propyleneglycol, dipropyleneglycol, 1,3-butyleneglycol, glycerin, methylpropandiol, isopreneglycol, pentyleneglycol, erythritol, xylitol and sorbitol; the surfactant is anionic surfactant selected from alkylacylglutamate, alkylphosphate, alkyllactylate, dialkylphosphate and trialkylphosphate or nonionic surfactant selected from alkoxylated alkylether, alkoxylated alkylester, alkylpolyglycoside, polyglycerylester and sugarester; the phospholipid is lecithin; the free fatty acid is C12-22 alkyl chain optionally saturated fatty acid; and the composition has a formulation selected from solution, emulsion, suspension, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powdered foundation, emulsified foundation, wax foundation and spray.

Description

Cosmetic composition for alleviating skin stimulation containing nanoliposomes of intercellular lipid components {{Cosmetic Composition for Alleviating Skin Irritation Comprising Nanoliposome of Intercellular Lipids}

The present invention relates to a cosmetic composition for alleviating skin irritation containing nanoliposomes, and more particularly to a cosmetic composition containing nanoliposomes which are essential components of ceramides, cholesterol and free fatty acids, which are intercellular lipids.

Generally, various raw materials are used to manufacture cosmetics, and typically 20 to 50 raw materials are used. These ingredients have been proven to be safe through skin irritation tests, but they can be applied to products that can cause irritation, itching and allergies to the skin. These reactions can happen to everyone, but only to specific people. Therefore, cosmetics manufacturers are making efforts to minimize the irritation by applying commonly used irritant substances to the product.

Skin irritation that can be caused by the use of cosmetics includes irritation such as itching, stinging, burning, erythema, edema, and irritant contact with contact dermatitis. Dermatitis, allergic contact dermatitis, sensory stimulation, phototoxic dermatitis can be roughly distinguished, but it is difficult to clearly distinguish them, and they are often mixed with each other. Above all, skin irritation may occur because of skin penetration by cosmetics and their immune system is activated through interaction with epidermis or dermal cells.

As such, the skin barrier that primarily prevents the penetration of external harmful substances exists in the stratum corneum of the epidermis. The function of the stratum corneum acts as a barrier to inhibit the loss of moisture and electrolyte out of the skin, preventing the epidermis from drying and the epidermis is normal. It provides an environment for biochemical metabolism, protects the body from external physical damage and chemicals, and prevents the invasion of bacteria, fungi and viruses into the skin.

The structure of the stratum corneum is composed of protein-rich keratinocytes and lipids filling them.In the mid-1970s, Elias et al. This is the source of skin barrier function ( Arch. Dermatol. Res. 27: 95-117 (1981)).

The structure of the stratum corneum is described as a two-compartment model, which is called the brick and mortar model in comparison to the mortar, which is the brick when the walls are stacked and the adhesive that is filled between them. Int. J. Dermatol. 20: 1-19 (1981). When the barrier function is weakened due to abnormalities of the stratum corneum of the skin, it is understood that various skin diseases occur or worsen.

Since the masonry and mortar models have been published, many studies have been carried out on lipid components between keratinocytes. It is recognized as important.

These intercellular lipids, in principle, consist of 50% ceramide, 20-25% cholesterol, 20-25% free fatty acids, 10% cholesterol esters and 1-2% cholesterol sulfate, with little or no phospholipids. A small amount is included.

That the lipids of the horny layer differs significantly from the other tissues of the living body is that the main constituent lipids ceramides, cholesterol, fatty acids and the like, the carbon number of the lipids that make up the ceramide shows a distribution of carbon as a relatively long-chain C ₁₆ -C ₃₃ . In addition, the carbon number of free fatty acids is also very high in C ₂₂ and C ₂₄ . Thus, the carbon distribution is much longer than that of the phospholipids present in the biological lipid membrane.

As such, the very long carbon distribution of the intercellular lipid species makes it difficult to penetrate these epidermal lipid species into the skin.

Meanwhile, studies using liposomes have been actively conducted by various researchers. However, most of its uses are "promoting transdermal absorption of active ingredients" (Korean Application Nos. 2000-0008212 and 2000-0042399), "Enhancing Skin Moisturizing" (Korean Application No. 1994-7004646), "Atopic Dermatitis" And improvement of dry skin ”(Korean Application No. 2000-0027165).

The present inventors earnestly researched to develop a cosmetic composition that can improve the function of the skin barrier and relieve skin irritation, and as a result, nanoliposomes containing ceramides, cholesterol and free fatty acids, which are the three major lipid species that form epidermal intercellular lipids This invention was completed by confirming that the cosmetic composition containing can exhibit the said effect.

Accordingly, it is an object of the present invention to provide a cosmetic composition containing nanoliposomes.

Another object of the present invention is to provide a cosmetic composition with reduced skin irritation of the active ingredient of the skin irritant.

Other objects and advantages of the present invention will become apparent from the following detailed description and claims.

According to one aspect of the present invention, the present invention provides a cosmetic composition containing a nanoliposome consisting of ceramides, cholesterol and free fatty acids as intercellular lipids as essential components.

Liposomes are defined as spherical phospholipid vesicles of colloidal particles that associate themselves. Liposomes composed of amphiphilic molecules with water-soluble heads (hydrophilic groups) and insoluble tails (hydrophobic groups) spontaneously bind and form an ordered structure by their interaction. It is classified into Small Unilamellar Vesicle (SUV), Large Unilamellar Vesicle (LUV) and Multi Lamellar Vesicle (MLV) according to its size and lamellarity. Such liposomes exhibiting various laminations have a double membrane structure similar to that of cell membranes. Therefore, the inventors of the present invention have determined that the intracellular lipid components of ceramide, cholesterol, and free fatty acids may be provided as small particle liposomes, that is, nanoliposomes, to facilitate their skin penetration.

The cosmetic composition of the present invention exhibits the action of (i) repairing the damaged skin barrier, (ii) strengthening the skin barrier, (i) alleviating skin irritation, or (v) a combination thereof.

Preferably, the intercellular lipids used in the nanoliposomes of the present invention are ceramide: cholesterol: free fatty acids having a weight ratio of (0.1-5) :( 0.1-5) :( 0.1-2), more preferably Is (1-4) :( 1-4) :( 0.2-2), and most preferably, it is a weight ratio of 2: 2: 1. According to a preferred embodiment of the present invention, the content of the nanoliposome is 0.1-20.0% by weight, more preferably 1.0-10.0% by weight relative to the total weight of the composition.

According to a preferred embodiment, the nanoliposomes of the present invention comprise (i) an oily component comprising polyols, (b) ceramides and cholesterol, (i) surfactants, (i) phospholipids, (i) free fatty acids and (i) water. It is characterized in that it is prepared by a mixture comprising, the mixture is characterized in that it does not contain a monohydric alcohol.

As used herein, the term "nanoliposomes" refers to liposomes having an average particle diameter of 1-100 nm as having the form of conventional liposomes. According to a preferred embodiment of the invention, the average particle diameter of the nanoliposomes is 30-70 nm. When the average particle diameter of the nanoliposomes exceeds 70 nm, the improvement of skin penetration and the stability of formulation is very weak among the technical effects to be achieved in the present invention.

The polyol used in the nanoliposome of the present invention is not particularly limited, and preferably, propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methyl propanediol, isoprene glycol, pentylene glycol, erythritol, xyitol, Sorbitol and mixtures thereof. It is preferably selected from the group consisting of propylene glycol, 1,3-butylene glycol, glycerin and mixtures thereof, most preferably glycerin. The amount used is 10-80% by weight, preferably 30-70% by weight based on the total weight of the nanoliposomes.

The oil component used in the preparation of the nanoliposome of the present invention may be various oils known in the art, preferably hydrocarbon-based oils such as hexadecane and paraffin oil; Synthetic synthetic oils; Silicone oils such as dimethicone and cyclomethicone series; Animal and vegetable oils such as sunflower oil, corn oil, soybean oil, avocado oil, sesame oil, jojoba oil, squalane and fish oil; Ethoxylated alkyl ether oils; Propoxylated alkyl ether oils; Sphingoidoid lipids such as phytosphingosine, sphingosine and sphinginine; Cerebroside; cholesterol; Cytosterol; Cholesteryl sulfate; Cytosteryl sulfate; C _10-40 fatty alcohols and ceramides. More preferably it is a mixture of ceramide, cholesterol and animal and vegetable oils (jojoba oil, squalane and the like). The amount used is 1.0-30.0% by weight, preferably 3.0-20.0% by weight based on the total weight of nanoliposomes. On the other hand, ceramides used in the present invention include all types up to type 1-type 6, most preferably ceramide type 3.

Surfactants used in the preparation of the nanoliposomes of the present invention can be used in the art known in the art, for example, from the group consisting of alkylacylglutamate, alkyl phosphate, alkyl lactylate, dialkyl phosphate and trialkyl phosphate Anionic surfactant selected or a nonionic surfactant selected from the group consisting of alkoxylatedalkylether, alkoxylatedalkylester, alkylpolyglycoside, polyglyceryl ester and sugar ester, most preferably, nonionic surfactant Polysorbates belonging to the active agent are used. The amount of the surfactant to be used is generally 0.1-10% by weight, preferably 0.2-3.0% by weight based on the total weight of the nanoliposomes.

Phospholipids, another component used in the preparation of the nanoliposomes of the present invention, are used as amphoteric lipids, including natural phospholipids (eg, egg yolk lecithin or soy lecithin, sphingomyelin) and synthetic phospholipids (eg dipalmitoyl). Phosphatidylcholine or hydrogenated lecithin), preferably lecithin. More preferably, the lecithin is naturally occurring unsaturated lecithin or saturated lecithin extracted from soybean or egg yolk. Typically, lecithin derived from nature has 23-95% phosphatidyl choline and 20% or less phosphadidylethanolamine. In the preparation of the nanoliposomes of the present invention, the amount of lecithin used is 0.5-20.0% by weight, preferably 2.0-10.0% by weight, based on the total weight of the nanoliposomes.

The fatty acids used in the preparation of the nanoliposomes of the present invention are higher fatty acids, preferably saturated or unsaturated fatty acids of a C _12-22 alkyl chain, such as lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid. And palmilean acid, more preferably is selected from the group consisting of stearic acid, palyoleic acid and mixtures thereof. The amount used is 0.05-5.0% by weight, preferably 0.1-4.0% by weight relative to the total weight of nanoliposomes.

Water used for the preparation of the nanoliposomes of the present invention is generally deionized distilled water, the amount of which is used is 5.0-40% by weight relative to the total weight of nanoliposomes.

Nanoliposomes of the present invention may further comprise emollients and most preferably include caprylic / capric triglycerides. The content is 1.0-15.0 wt%, preferably 3.0-7.0 wt%. In addition, the nanoliposomes of the present invention may optionally comprise small amounts (about 0.5-1.0% by weight) of emulsions (eg, sodium stearoyl lactate).

The preparation of nanoliposomes can be accomplished through various methods known in the art, but most preferably, a mixture comprising the above components is applied to a high pressure homogenizer. The preparation of nanoliposomes by high pressure homogenizer can be carried out under various conditions (eg pressure, frequency, etc.) depending on the desired particle size, preferably 1-5 times high pressure homogenizer under 600-1200 bar pressure. Nanoliposomes are prepared by passing through.

The components included in the cosmetic composition of the present invention include components conventionally used in cosmetic compositions, in addition to nanoliposomes containing intercellular lipids as active ingredients, and include, for example, conventional agents such as stabilizers, solubilizers, vitamins, pigments and perfumes. Adjuvants, and carriers.

The cosmetic composition for protecting the skin of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactants- It may be formulated as containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, spray, and the like, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition of the present invention facilitates skin penetration of intercellular lipid species, improves skin barrier function, and enhances skin moisturizing power by decreasing transdermal moisture loss, thereby greatly reducing skin irritation effect of cosmetic use. great.

Therefore, according to another aspect of the present invention, the present invention provides a cosmetic composition with reduced skin irritation of the skin irritant active ingredient comprising the cosmetic composition containing the nanoliposomes described above and a skin irritant active ingredient.

In the cosmetics industry, in order to impart various functionalities to the skin, many active ingredients are added and used in the cosmetic composition, but many of these ingredients have problems that cause skin irritation.

The cosmetic composition of the present invention is very useful for solving the problem of skin irritation. Representative among the active ingredients in which skin irritation can be alleviated by the cosmetic composition of the present invention are lactic acid, glycolic acid, salicylic acid and retinoic acid.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it is to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. Will be self-evident.

Preparation Example: Preparation of Nanoliposomes

Prize ingredient Content (% by weight) Production Example-1 Preparation Example-2 Preparation Example-3 Preparation Example-4 Preparation Example-5 Preparation Example-6 Preparation Example-7 Preparation Example-8 Preparation Example-9 Preparation Example-10 A glycerin 65.0 65.0 65.0 0.0 0.0 30.0 60.0 60.0 60.0 60.0 1,3-butylene glycol 0.0 0.0 0.0 60.0 0.0 40.0 0.0 0.0 0.0 0.0 Propylene glycol 0.0 0.0 0.0 0.0 60.0 0.0 0.0 0.0 0.0 0.0 B lecithin 5.0 5.0 5.0 8.0 0.0 0.0 0.0 6.0 3.0 3.0 Immersion Lecithin 0.0 0.0 0.0 0.0 6.0 8.0 8.0 0.0 3.0 3.0 cholesterol 1.0 7.0 4.0 4.0 4.0 3.0 3.0 3.0 5.0 5.0 Ceramide 7.0 1.0 4.0 4.0 4.0 3.0 6.0 6.0 5.0 5.0 Stearic acid 2.0 2.0 2.0 1.0 0.0 0.0 2.0 2.0 2.0 1.0 Palmitic Oleic Acid 0.0 0.0 0.0 1.0 2.0 2.0 0.0 0.0 1.0 2.0 Caprylic / Capric Triglycerides 5.0 5.0 5.0 5.0 0.0 0.0 5.0 5.0 5.0 8.0 Jojoba oil 0.0 0.0 0.0 0.0 0.0 3.0 0.0 5.0 0.0 0.0 Squalane 0.0 0.0 0.0 0.0 10.0 0.0 5.0 0.0 0.0 0.0 Sodium stearoyl lactate 0.5 1.0 0.5 1.0 0.5 0.5 0.5 1.0 0.0 0.5 Polysorbate-80 0.5 0.0 0.5 0.5 0.5 0.0 0.5 0.0 1.0 0.5 C Potassium hydroxide Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Purified water To.100 To.100 To.100 To.100 To.100 To.100 To.100 To.100 To.100 To.100

Phase B was added to phase A and wetted uniformly, phase C was then added to AB and heated and mixed to 70-77 ° C. and stirred uniformly. Subsequently, the mixture was passed through a high pressure homogenizer 1-5 times under conditions of 600-1,200 bar and cooled to prepare nanoliposomes having a particle size of 30-70 nm in diameter.

Examples and Comparative Examples

Compositions containing nanoliposomes stabilized intercellular lipids of the present invention (Examples) and compositions without nanoliposomes (Comparative Examples) were prepared in the following compositions.

ingredient Example Comparative Example 1 Comparative Example 2 Content (% by weight) Content (% by weight) Content (% by weight) Nanoliposomes (Preparation Example-3) 10.0 - - cholesterol - 0.4 - Ceramide - 0.4 - Stearic acid - 0.2 - A Cetostearyl alcohol 2.0 2.0 2.0 Glyceryl Stearate 1.5 1.5 1.5 Microcrystalline Wax 0.7 0.7 0.7 Squalane 5.0 5.0 5.0 Liquid paraffin 3.0 3.0 3.0 Trioctanoine 5.0 5.0 5.0 Polysorbate 1.2 1.2 1.2 Sorbitan stearate 0.5 0.5 0.5 Tocopheryl Acetate 0.2 0.2 0.2 Cyclomethicone 3.0 3.0 3.0 BHT 0.05 0.05 0.05 B glycerin 4.0 4.0 4.0 1,3-butylene glycol 2.0 2.0 2.0 EDTA-2Na 0.05 0.05 0.05 Purified water To 100 To 100 To 100 Incense, preservative Quantity Quantity Quantity

Experimental Example 1: Measurement of the stability of the formulation

The stability test for the cosmetic containing the nanoliposomes stabilized the intercellular lipids of the present invention was carried out by the following method.

In order to test the stability of the cosmetics Examples and Comparative Examples 1 and 2 were kept in an opaque glass container in a constant temperature bath at a constant temperature of 45 ℃ stored for 12 weeks in a completely shaded refrigerator also kept constant at 4 ℃ After storage for 12 weeks in an opaque glass container, the degree of recrystallization was measured. The results are summarized in Table 3 below. At this time, the degree of product crystallization was evaluated by classifying into the following six grades. 0: no change; 1: extremely little crystallization; 2: little crystallization; 3: a little bit severe crystallization; 4: severely crystallized; And 5: extremely heavily crystallized.

Temperature Degree of crystallization Example Comparative Example 1 Comparative Example 2 45 ℃ 0 1.8 0 Room temperature 0 2.6 0 4 ℃ 0 4.3 0

As can be seen from Table 3, the Example was stable without any crystallization under all temperature conditions, but Comparative Example 1 used directly in the formulation without stabilizing intercellular lipids crystallization of lipids at all temperature conditions, especially at low temperatures It is even worse that the formulation itself is unstable.

Experimental Example 2: Recovery test after skin barrier injury in hairless mice

Acetone was applied as a method of skin barrier damage. When transepidermal water loss (TEWL) reaches 4.0 g / m ² / h by dropping acetone into the embryos of 8-12 week old hairless mice, the examples of Table 2 and Comparative Examples 1 and 2 Samples were applied to a 5 cm ² area. TEWL was measured with Tewameter TM 210, an evaporimeter from CK (Courage + Khazaka, Cologne, Germany). After 6 hours of application, TEWL was measured to evaluate the extent to which TEWL was reduced to evaluate the extent to which skin barrier function was restored. Recovery rate calculation used in the evaluation was calculated as in the following equation 1 and the results are shown in Table 4.

Br (Barrier Recovery) = [1- (B _{t = 6} -B _{t = 0} ) / (B _{t = d} -B _{t = 0} )] x 100

B _{t = 6} : TEWL measured 6 hours after skin barrier injury

B _{t = 0} : TEWL measurement before skin barrier injury

B _{t = d} : TEWL measured immediately after skin barrier injury

Application Sample 6 hours elapsed (based on initial TEWL) Example Comparative Example 1 Comparative Example 2 ^{62.1 g / m 2 /h24.5 g /} m 2 /h12.4 g / m 2 / h

Looking at the results of Table 4, in the case of the Example containing nanoliposomes stabilized intercellular lipids, compared to the case of Comparative Example 1 containing intercellular lipids, Comparative Example 2 does not contain all the recovery effect after skin barrier damage It can be seen that is very excellent.

Experimental Example 2: Evaluation of Water Retention Capacity and Water Evaporation Rate in Human Body Application

In order to evaluate the moisturizing power of each cosmetic composition prepared in Examples and Comparative Examples 1 and 2, the moisture retention capacity and the amount of water evaporation were measured as follows on the skin to which each cosmetic was applied.

Experimental Example 2-1: Measurement of Water Retention Capacity

In the constant temperature and humidity room of 22 ° C. and 45% RH, each cosmetic composition prepared in Examples and Comparative Examples 1 and 2 was coated with a predetermined amount (0.03 g / 16 cm 2) in the lower half of 30 test subjects, and before application, The moisture content of the skin after 1 hour of application and after 2 hours of application was measured. The measuring device used a moisture content measuring instrument (corneometer CM820, Courage + Khazaka, Cologne, Germany) to measure the moisturizing power by measuring the electric capacity of the skin according to the moisture content of the skin. The results are shown in Table 5 below.

division Example Comparative Example 1 Comparative Example 2 Skin electrical conductivity before application 50 50 50 Skin electrical conductivity 1 hour after application 85 72 50 Skin electrical conductivity 2 hours after application 77 56 50

Experimental Example 2-2: Measurement of Water Evaporation Amount

After tape stripping on the upper arm of the arm to weaken the skin's protective film, apply the cosmetics to the subject in the same manner as in Experiment 2-1, and then apply CK (Courage + Khazaka, Cologne, Germany). Using Tewameter TM 210, an evaporator, the water vapor evaporation was measured 5 times at 1 minute intervals every 12 hours to obtain an average value. The results are shown in Table 6 below.

  division Example (%) Comparative Example 1 (%) Comparative Example 2 (%) Moisture evaporation after 0 hours 100.0 100.0 100.0 Moisture evaporation after 12 hours 63.1 92.5 95.1 Moisture evaporation after 24 hours 43.2 83.0 90.3 Moisture evaporation after 36 hours 43.0 71.5 81.1 Moisture evaporation after 48 hours 40.3 59.0 73.0

As shown in the results of Experimental Examples 2-1 and 2-2, the Example cosmetics containing the nanoliposomes stabilized intercellular lipids of the present invention have a higher electrical conductivity of the skin than the cosmetics of the Comparative Example, and a small amount of water loss. Indicates. Therefore, it can be seen that the cosmetic of the present invention exhibits an excellent moisturizing effect.

Experimental Example 3: Anti-inflammatory effect evaluation method-Carrageenan foot edema

In order to confirm whether the cosmetic of the present invention exhibits an excellent anti-inflammatory effect, 1 hour after administration of 10-100 mg / kg of each of Examples and Comparative Examples 1 and 2 to the mouse abdominal cavity, 0.5 ml of 0.1% carrageenan solution was used in experimental animals Inflammation was induced by injection into the sole of the foot. The change in the paw volume of the rat immediately after carrageenan injection and 4 hours after the injection was measured and the edema inhibition rate was calculated according to the following Equation 2 and is shown in Table 7 below.

% Inhibition = [(1-ΔV treated group) / ΔV control] x 100

(ΔV: change of foot volume)

Dosage (mg) % Inhibition Rate Example Comparative Example 1 Comparative Example 2 10 22.3 11.6 2.8 30 37.9 23.7 7.8 50 59.8 43.6 10.7 100 62.9 51.5 11.6

As shown in the results of Table 7, it can be seen that the example cosmetic containing the nanoliposomes stabilized intercellular lipids of the present invention exhibited an excellent anti-inflammatory effect compared to the cosmetic of the comparative example.

Experimental Example 4: Human Skin Patch Test

In the past, the skin irritation was not sensitized in the past, currently skin irritation or skin allergy in 20-30s women 20-30 women, lactic acid 5.0% skin irritant was added as an irritant, Example 1 , 2 was confirmed through the skin patch test to reduce the irritation effect.

First the test site was wiped with 70% ethanol and dried. 15 μg of the prepared test substance was added dropwise into a Fin chamber (Finn chamber, 100 × 10, EPITEST, Finland) and then airtightly wrapped inside the forearm of the subject. The patch was applied for 24 hours, the patch was removed, and the test site was marked with a marker pen. After 1 hour and 24 hours, magnification (8MC-150, DAZOR, United States of America) was used to observe the test site, and erythema and edema were observed.

Skin reactions were determined according to the regulations of the International Contact Dermatitis Research Group (ICDRG), and the results are shown in Table 8.

-Evaluation criteria and score of skin reaction-

     Symbol Score Criteria

       -0 no response

       ± 0.5 faint or light erythema

       + 1 clear but weak erythema, edema and papules

       ++ 2 distinct erythema, papules and vesicles

       +++ 3 Severe erythema and alveolar, scleroderma

Mean response rate = [(score x responses x 100 x 1/2) / (3 x (maximum score) x total number of subjects)]

Test substance 24 hours 48 hours Skin reactivity (%) (n = 20)  ± + ++ ± + ++ Example + 5% Lactic Acid Comparison Example 1 + 5% Lactic Acid Comparison Example 2 + 5% Lactic Acid 4 1 -6 1 -11 3- 1 0 -2 1 -4 2- 1.482.505.00

As can be seen from Table 8, it can be seen that the cosmetic composition containing the nanoliposomes stabilized intercellular lipids of the present invention is superior to the skin irritation effect induced by 5% lactic acid as compared to the cosmetics of the comparative example.

Experimental Example 5: Comparison of skin irritation relief effect in human application

20 women in their 20s and 30s were used for 10 days with Examples and Comparative Examples 1 and 2 containing 5.0% of a skin irritant, lactic acid. Each morning and evening were used, and then questionnaires were marked for irritation and intensity. The test results were evaluated once a day, and divided into subjective and objective stimuli, and the results are shown in Table 9 below.

Skin irritation relief rate Test substance Subjective stimulus (% relaxation) Objective stimulation (% relaxation) Total (% relaxation) Example 49 62 55 Comparative Example 1 24 29 26 Comparative Example 2 7 11 9

As can be seen in Table 9, the cosmetic composition of the present invention containing the nanoliposomes stabilized intercellular lipids of the present invention is excellent in alleviating the subjective and objective stimulation induced by 5% lactic acid compared to the cosmetic of the comparative example Able to know.

Hereinafter, based on the results of the above experimental example, to present a cosmetic composition for alleviating skin irritation containing a nanoliposome stabilized intercellular lipids of the present invention. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Formulation Example 1: Softener (Skin Lotion)

As shown in Table 10 below, the flexible longevity was prepared according to a conventional method.

Compounding ingredient Content (% by weight) Nano liposome 3.0 1.3-butylene glycol 6.0 glycerin 4.0 EDTA-2Na 0.02 Sodium Hiruronate 2.0 Fiji-40 Hydrogenated Castor Oil 0.5 ethanol 15.0 Benzophenone-9 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example 2. Nutrients (Milk Lotion)

As shown in Table 11, nutrient lotion was prepared according to a conventional method.

Compounding ingredient Content (% by weight) Nano liposome 5.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocopheryl Acetate 3.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxy Vinyl Polymer 0.3 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

Formulation Example 3: Nutrition Cream

As shown in Table 12, nutrition cream was prepared according to a conventional method.

Compounding ingredient Content (% by weight) Nano liposome 15.0 Cetostearyl alcohol 2.5 Glyceryl Stearate 1.5 Trioctanoine 5.0 Polysorbate 60 1.2 Sorbitan stearate 0.5 Squalane 5.0 Floating paraffin 3.0 Cyclomethicone 3.0 BH 0.05 Delta-tocopherol 0.2 glycerin 4.0 1,3-butylene glycol 2.0 Xanthan Gum 0.2 EDTA-2Na 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example 4: Massage Cream

Massage cream was prepared according to a conventional method as shown in Table 13.

Compounding ingredient Content (% by weight) Nano liposome 5.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Beeswax 2.0 Tocopheryl Acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Carboxy Vinyl Polymer 0.5 Incense, preservative a very small amount Purified water to 100

Formulation Example 5: Pack

Packs were prepared according to conventional methods as shown in Table 14 below.

Compounding ingredient Content (% by weight) Nano liposome 2.0 Propylene glycol 2.0 glycerin 4.0 Carboxy Vinyl Polymer 0.3 ethanol 7.0 Fiji-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

As described in detail above, the present invention provides a cosmetic composition containing nanoliposomes. The present invention also provides a cosmetic composition with reduced skin irritation of the active ingredient of the skin irritant. The cosmetic composition of the present invention facilitates skin penetration of intercellular lipid species, improves skin barrier function, and enhances skin moisturizing power by decreasing transdermal moisture loss, thereby greatly reducing skin irritation effect of cosmetic use. great.

Claims (14)

A cosmetic composition containing a nanoliposome having essential components of ceramide, cholesterol and free fatty acids, which are intercellular lipids. The nanoliposome according to claim 1, wherein the cosmetic composition exhibits the action of (i) repairing the damaged skin barrier, (ii) strengthening the skin barrier, (i) alleviating skin irritation, or (v) a combination thereof. Cosmetic composition containing a. The cosmetic composition of claim 1, wherein the content of the nanoliposome is 0.1-20.0 wt% with respect to the total weight of the composition. The method of claim 1, wherein the nanoliposome comprises (a) an oily component comprising polyol, (b) ceramide and cholesterol, (i) surfactant, (i) phospholipid, (i) free fatty acid and (i) water It characterized in that it is prepared by a mixture, wherein the mixture is a cosmetic composition containing a nano liposome, characterized in that it does not contain a monohydric alcohol. The method according to claim 4, wherein the polyol is selected from the group consisting of propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methylpropanediol, isoprene glycol, pentylene glycol, erythritol, xyitol and sorbitol The content of the cosmetic composition containing a nano-liposomes, characterized in that 30 to 70% by weight relative to the total weight of nano-liposomes. The oil-based component according to claim 4, wherein the oil component is hydrocarbon oil, ester oil, silicone oil, animal and vegetable oil, ethoxylated alkyl ether oil, propoxylated alkyl ether oil, in addition to ceramide and cholesterol. , And further selected from the group consisting of sphingoidoid lipids, ceramides, cerbrosides, cholesterol, cytosterols, cholesteryl sulfates, cytosterylsulfates, C _10-40 fatty alcohols and mixtures thereof, the content of which is nano Cosmetic composition containing a nano liposome, characterized in that 3 to 20% by weight relative to the total liposome weight. The method of claim 4, wherein the surfactant is an anionic surfactant or alkoxylated alkyl ether, alkoxylated alkyl ester selected from the group consisting of alkylacyl glutamate, alkyl phosphate, alkyl lactylate, dialkyl phosphate and trialkyl phosphate, A nonionic surfactant selected from the group consisting of alkyl polyglycosides, polyglyceryl esters and sugar esters, the content of which is a cosmetic composition containing nanoliposomes, characterized in that the content of 0.2-3.0% by weight relative to the total weight of nanoliposomes. . The cosmetic composition according to claim 4, wherein the phospholipid is lecithin and its content is 2-10% by weight based on the total weight of the nanoliposomes. 9. The cosmetic composition according to claim 8, wherein the lecithin is a naturally occurring unsaturated lecithin or saturated lecithin extracted from soybean or egg yolk. 5. The cosmetic composition according to claim 4, wherein the free fatty acid is a saturated or unsaturated fatty acid of a C _12-22 alkyl chain, the content of which is 0.1-4.0 wt% based on the total weight of the nanoliposomes. The cosmetic composition of claim 4, wherein the nanoliposomes are prepared by applying the mixture to a high pressure homogenizer. The composition according to claim 1, wherein the composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation Cosmetic composition containing nanoliposomes, characterized in that having a formulation selected from the group consisting of, wax foundation and spray. A cosmetic composition having reduced skin irritation of the skin irritant active ingredient, comprising the cosmetic composition of any one of claims 1 to 11 and an active ingredient having skin irritation. 14. The cosmetic composition with reduced skin irritation according to claim 13, wherein the active ingredient for skin irritation is lactic acid, glycolic acid, salicylic acid or retinoic acid.