KR20040108672A - 올리고뉴클레오티드 및 그 유사체의 정제방법 - Google Patents

올리고뉴클레오티드 및 그 유사체의 정제방법 Download PDF

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Publication number
KR20040108672A
KR20040108672A KR10-2004-7014747A KR20047014747A KR20040108672A KR 20040108672 A KR20040108672 A KR 20040108672A KR 20047014747 A KR20047014747 A KR 20047014747A KR 20040108672 A KR20040108672 A KR 20040108672A
Authority
KR
South Korea
Prior art keywords
target oligonucleotide
oligonucleotides
anion exchange
oligonucleotide
target
Prior art date
Application number
KR10-2004-7014747A
Other languages
English (en)
Korean (ko)
Inventor
조한센잭티.
Original Assignee
아베시아 바이오테크놀러지, 아이엔씨.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 아베시아 바이오테크놀러지, 아이엔씨. filed Critical 아베시아 바이오테크놀러지, 아이엔씨.
Publication of KR20040108672A publication Critical patent/KR20040108672A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR10-2004-7014747A 2002-03-21 2003-03-19 올리고뉴클레오티드 및 그 유사체의 정제방법 KR20040108672A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US36706002P 2002-03-21 2002-03-21
US60/367,060 2002-03-21
PCT/GB2003/001161 WO2003080834A2 (en) 2002-03-21 2003-03-19 Purification methods for oligonucleotides and their analogs

Publications (1)

Publication Number Publication Date
KR20040108672A true KR20040108672A (ko) 2004-12-24

Family

ID=28454833

Family Applications (1)

Application Number Title Priority Date Filing Date
KR10-2004-7014747A KR20040108672A (ko) 2002-03-21 2003-03-19 올리고뉴클레오티드 및 그 유사체의 정제방법

Country Status (7)

Country Link
EP (1) EP1490488A2 (de)
JP (1) JP2005520547A (de)
KR (1) KR20040108672A (de)
CN (1) CN1643145A (de)
AU (1) AU2003216830A1 (de)
CA (1) CA2479901A1 (de)
WO (1) WO2003080834A2 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006132588A1 (en) * 2005-06-10 2006-12-14 Quiatech Ab Method for the purification of synthetic oligonucleotides containing one or several labels
AU2012205204B2 (en) * 2005-12-09 2015-03-12 Qiagen Gmbh Method for enriching short-chain nucleic acids
DE102005059315A1 (de) * 2005-12-09 2007-06-14 Qiagen Gmbh Verfahren zur Anreicherung von kurzkettigen Nukleinsäuren
GB2445441B (en) 2006-09-26 2010-06-30 Ge Healthcare Bio Sciences Nucleic acid purification method
GB2443505B (en) 2006-09-26 2008-12-31 Ge Healthcare Bio Sciences Nucleic acid purification method
GB2445442A (en) * 2006-09-26 2008-07-09 Ge Healthcare Bio Sciences Nucleic acid purification using anion exchange
DE102008063003A1 (de) 2008-12-23 2010-06-24 Qiagen Gmbh Nukleinsäureaufreinigungsverfahren
JP5914338B2 (ja) * 2009-09-24 2016-05-11 キアジェン ゲイサーズバーグ インコーポレイテッド 陰イオン交換材料を使用した核酸の単離および分析のための組成物、方法およびキット
CN102753705A (zh) * 2009-12-14 2012-10-24 B·吴 分离核酸物质的方法和材料
EP2760999A1 (de) * 2011-09-26 2014-08-06 Qiagen GmbH Verfahren zur trennung von nukleinsäuren nach grösse
CN103833797A (zh) * 2012-11-26 2014-06-04 福建金山生物制药股份有限公司 一种纯化硫代寡核苷酸的工业化方法
US10745686B2 (en) 2013-02-08 2020-08-18 Qiagen Gmbh Method for separating DNA by size
CN106632558B (zh) * 2016-08-25 2019-09-13 淮阴师范学院 一种寡聚核苷酸的纯化方法及应用
MX2021002745A (es) * 2018-09-11 2021-05-12 Amgen Inc Metodos de purificacion de oligonucleotidos ricos en guanina.
JP7311642B2 (ja) * 2019-07-09 2023-07-19 エフ． ホフマン－ラ ロシュ アーゲー オリゴヌクレオチドを脱保護するための方法
CN114787622A (zh) * 2019-12-05 2022-07-22 沃特世科技公司 用于提高回收率并将系统损失减到最小的聚膦酸
CN111394427B (zh) * 2020-04-22 2023-04-11 宁波市博坤生物科技有限公司 一种含泥土检材中微量dna的提取方法
WO2022015801A1 (en) * 2020-07-14 2022-01-20 Waters Technologies Corporation Kits and methods useful for separating oligonucleotides from matrix components
WO2024089953A1 (ja) * 2022-10-27 2024-05-02 住友化学株式会社 オリゴヌクレオチドの製造方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2286795A (en) * 1994-04-08 1995-10-30 Hybridon, Inc. Purification of oligodeoxynucleotide phosphorothioates using anion exchange chromatography
US6310199B1 (en) * 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
GB9927904D0 (en) * 1999-11-25 2000-01-26 Amersham Pharm Biotech Ab A method fro obtaining a nucleic acid variant
DE10010342A1 (de) * 2000-03-06 2001-09-20 Merck Patent Gmbh Verfahren zur Abreicherung von Endotoxinen

Also Published As

Publication number Publication date
CN1643145A (zh) 2005-07-20
JP2005520547A (ja) 2005-07-14
AU2003216830A1 (en) 2003-10-08
EP1490488A2 (de) 2004-12-29
WO2003080834A2 (en) 2003-10-02
CA2479901A1 (en) 2003-10-02
WO2003080834A3 (en) 2003-12-31

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