MX2021002745A - Metodos de purificacion de oligonucleotidos ricos en guanina. - Google Patents

Metodos de purificacion de oligonucleotidos ricos en guanina.

Info

Publication number
MX2021002745A
MX2021002745A MX2021002745A MX2021002745A MX2021002745A MX 2021002745 A MX2021002745 A MX 2021002745A MX 2021002745 A MX2021002745 A MX 2021002745A MX 2021002745 A MX2021002745 A MX 2021002745A MX 2021002745 A MX2021002745 A MX 2021002745A
Authority
MX
Mexico
Prior art keywords
guanine
quadruplex
oligonucleotides
purification methods
rich oligonucleotides
Prior art date
Application number
MX2021002745A
Other languages
English (en)
Inventor
Artaches Kazarian
Wesley Barnhart
Iain David Grant Campuzano
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of MX2021002745A publication Critical patent/MX2021002745A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/712—Nucleic acids or oligonucleotides having modified sugars, i.e. other than ribose or 2'-deoxyribose
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/166—Fluid composition conditioning, e.g. gradient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/323—Chemical structure of the sugar modified ring structure
    • C12N2310/3231—Chemical structure of the sugar modified ring structure having an additional ring, e.g. LNA, ENA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere a métodos para purificar ácidos nucleicos. En particular, la presente invención se refiere a un método para separar oligonucleótidos ricos en guanina de estructuras secundarias en cuádruplex formadas a partir de oligonucleótidos usando cromatografía de intercambio aniónico monolítico. También se describen los parámetros de fase móvil que controlan la formación cuádruplex y permiten la separación del cuádruplex intacto del oligonucleótido monocatenario.
MX2021002745A 2018-09-11 2019-09-10 Metodos de purificacion de oligonucleotidos ricos en guanina. MX2021002745A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862729878P 2018-09-11 2018-09-11
PCT/US2019/050483 WO2020055922A1 (en) 2018-09-11 2019-09-10 Purification methods for guanine-rich oligonucleotides

Publications (1)

Publication Number Publication Date
MX2021002745A true MX2021002745A (es) 2021-05-12

Family

ID=68051971

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021002745A MX2021002745A (es) 2018-09-11 2019-09-10 Metodos de purificacion de oligonucleotidos ricos en guanina.

Country Status (7)

Country Link
US (1) US12049620B2 (es)
EP (1) EP3850096A1 (es)
JP (1) JP7532342B2 (es)
AU (1) AU2019340388A1 (es)
CA (1) CA3110524A1 (es)
MX (1) MX2021002745A (es)
WO (1) WO2020055922A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4070102A1 (en) 2019-12-05 2022-10-12 Waters Technologies Corporation Polyphosphonic acids for improving recovery and minimizing system loss
CN116209757A (zh) * 2020-07-14 2023-06-02 沃特世科技公司 用于从基质组分中分离寡核苷酸的组合物、试剂盒和方法
TW202323526A (zh) * 2021-09-30 2023-06-16 美商安進公司 分離富含鳥嘌呤的寡核苷酸的分子物質之方法
EP4419904A1 (en) * 2021-10-21 2024-08-28 Waters Technologies Corporation Methods for anion-exchange analysis of nucleic acids
CN115728406A (zh) * 2022-08-05 2023-03-03 怡道生物科技(苏州)有限公司 硫代寡聚核苷酸的液相色谱分离方法及其应用
CN116718701B (zh) * 2023-06-21 2024-09-17 北京悦康科创医药科技股份有限公司 一种Inclisiran药物中间体纯度的检测方法

Family Cites Families (15)

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Publication number Priority date Publication date Assignee Title
US5539082A (en) 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
US5719262A (en) 1993-11-22 1998-02-17 Buchardt, Deceased; Ole Peptide nucleic acids having amino acid side chains
US5714331A (en) 1991-05-24 1998-02-03 Buchardt, Deceased; Ole Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility
US5506103A (en) * 1993-11-16 1996-04-09 Hybridon, Inc. Method for detecting charged oligonucleotides in biological fluids
EP0755400A1 (en) * 1994-04-08 1997-01-29 HYBRIDON, Inc. Purification of oligodeoxynucleotide phosphorothioates using anion exchange chromatography
US20050136458A1 (en) * 1998-12-31 2005-06-23 Oligos, Etc, Inc. Method for nucleic acid preparation
WO2001027331A2 (en) * 1999-10-12 2001-04-19 Transgenomic, Inc. Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography
US6693187B1 (en) 2000-10-17 2004-02-17 Lievre Cornu Llc Phosphinoamidite carboxlates and analogs thereof in the synthesis of oligonucleotides having reduced internucleotide charge
US9181551B2 (en) 2002-02-20 2015-11-10 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
AU2003216830A1 (en) 2002-03-21 2003-10-08 Avecia Biotechnology Inc. Purification methods for oligonucleotides and their analogs
US20050288246A1 (en) 2004-05-24 2005-12-29 Iversen Patrick L Peptide conjugated, inosine-substituted antisense oligomer compound and method
CN101611048B (zh) * 2006-12-12 2012-11-07 赛托斯生物技术公司 含有高浓度鸟嘌呤单体的寡核苷酸
US20140243216A1 (en) * 2011-09-26 2014-08-28 Qiagen Gmbh Methods for separating nucleic acids by size
EP2983804A4 (en) 2013-03-15 2017-03-01 Moderna Therapeutics, Inc. Ion exchange purification of mrna
EP3730619A1 (en) 2013-06-21 2020-10-28 Ionis Pharmaceuticals, Inc. Compositions and methods for modulation of target nucleic acids

Also Published As

Publication number Publication date
AU2019340388A1 (en) 2021-03-18
US12049620B2 (en) 2024-07-30
JP2022500025A (ja) 2022-01-04
WO2020055922A1 (en) 2020-03-19
JP7532342B2 (ja) 2024-08-13
CA3110524A1 (en) 2020-03-19
EP3850096A1 (en) 2021-07-21
US20220049240A1 (en) 2022-02-17

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