KR20040087236A - Mass culture of mycelium(mushroom) using Opuntia ficus-indica var. saboten as a substrate and the produced mycelium(mushroom) - Google Patents

Abstract

PURPOSE: A method for the mass culture of mushroom mycelium by inoculating mushroom mycelium into fruit or stem powder of Opuntia ficus-indica var. saboten and then culturing is provided. Therefore, the mushroom mycelium has functionality such as antioxidant effect, anti-aging effect and bronchial asthma-preventing effect or the like except for the original physiological effect of the mushroom mycelium. CONSTITUTION: About 0 to 50% by weight of water is added to fruit or stem powder of Opuntia ficus-indica var. saboten and sterilized at 120deg.C and 1.5atm for 20min. Then, 5%(v/v) of mushroom mycelium is inoculated into the powder of Opuntia ficus-indica and cultured at 25 to 30deg.C for 6 to 8 days. In the case of using freeze dried powder of Opuntia ficus-indica as culture medium, 5 to 10%(v/v) of water is added.

Description

Mass culture of mushroom mycelium using palm cactus and its mushroom mycelium {Mass culture of mycelium (mushroom) using Opuntia ficus-indica var. saboten as a substrate and the produced mycelium (mushroom)}

Palm cactus is designated as Jeju Island Local Monument No. 35, and its fruit and stem have long been known for its excellent constipation treatment, diuretic effect, activation of bowel movement, fever and detoxification. In addition, many studies have been conducted to cultivate Jeju's tourism specialty, which is known to be effective when dysentery, hemorrhoids, coughs, colds, sore throat, lung abscess, purulent mastitis, blisters, blister burns and snake bites. Palm cactus contains 5% of phenolic substances and flavonoids, which have anti-aging, anticancer and antimutagenic effects, and vitamin C is 5 times more than allore. In addition, it contains a large amount of calcium, which is necessary for the composition of the teeth and bones of our body, compared to the threshing plants, and from these characteristics, there is a great advantage in various processing and use of palm cactus as a raw material.

Recently, domestic patent applications related to palm cactus include the development of health foods (powdered tea, beverages, confectionery, etc.) based on palm cactus, the production of kimchi added with white lotus (fruit of palm cactus), immune enhancing agent, antibacterial agent, Aquaculture feed additives, functional soap production, water-soluble polysaccharide production, and the like.

Among these contents, the palm cactus mentioned in this patent has been patented. Functional food production using the bottom cactus as a hand (Korea Patent Application No. 1020010089955), aquaculture feed additive development (1020020017488), immune enhancing agent development (1020020092021), antibacterial agent Development (1020020072130), development of functional beverages (1019990075837), production of functional soaps (1020010002346), extraction and production of water-soluble polysaccharides (1019970042600) and the like have been reported. The palm cactus is one of the cacti cultivated in southern Korea . Saboten Makino (aka prickly pear) is a plant that is not only easy to cultivate but also has a labor cost of 1/4 to 1/5 compared to other crops. And there is an advantage that does not need treatment such as fertilizers or pesticides during tillage. Therefore, the present invention has been developed a method of using the mushroom mycelium as a culture substrate to contribute to the expansion of consumption and health promotion of the palm cactus.

The present invention provides a method for culturing mushroom mycelium, which is capable of economically cultivating mushroom mycelium and producing a culture containing the functionality of palm cactus in the culture medium, thereby allowing the extraction of a bioactive substance from the culture. The cultured mycelium and the culture solution are used as raw materials for processed foods and functional foods.

1 is a culture production process diagram of mushroom mycelium.

The present invention relates to the liquid culture of mushroom mycelium. Opuntia ficus-indica var.saboten , the 35th monument of Jeju Island, was born on the coast of Wolyeong-ri, Hallim-eup, Bukjeju-gun, along the current, and has been grown for about 200 years in the natural state. Before the cultivation began, it grew rapidly before the first decade, and in December 1998, the cactus cultivation area reached 350 ha. Currently, cactus fruit, stems, etc. can be easily obtained from Jeju Island, and the countermeasures for increasing consumption are being set up due to the increase in their production. The present invention is to cultivate mushroom mycelium with high efficiency using cactus fruit, stem or lyophilized powder thereof which can be continuously produced, extract functional materials from culture and use them as raw materials for food, functional food, etc. .

Mushroom mycelium culture method according to the present invention is as follows.

The strain is a mushroom mycelium.

Strain passaging is performed by dissolving 20 g of Potato Dextrose Agar Medium (PDA) in 1 liter of distilled water and sterilizing at high temperature and high pressure at 120 ° C and 1.5 atm for 20 minutes. Make and inoculate.

The preculture liquid medium dissolves 4 g of yeast extract, 1 g of malt extract, 2 g of glucose, 4 g of cactus fruit or stem grind (0.4 g in case of freeze-dried powder) in 1 liter of distilled water and adjusts the pH to 6.0. It is adjusted to, and sterilized at high temperature, high pressure and sterilized at 120 ° C. and 1.5 atm for 20 minutes, and used as a medium for preculture.

This culture liquid medium is mixed with water from the palm cactus fruit or stem grinding liquid available commercially in Jeju year-round, wherein the mixing ratio of the grinding liquid: water ranges from 50:50 to 100: 0. When lyophilized powder is used, the mixing ratio of powder to water is mixed in the range of 5:95 to 10:90, followed by pH control (6.0) and high temperature and high pressure wet sterilization (120 ° C., 1.5 atm, 20 minutes). Used as.

Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited by Examples.

Example 1 Mushroom Mycelial Culture

Mushroom mycelium culture process is shown in FIG.

Cactus fruit or stem is washed and crushed. There is no need to add water to the mill, and if added, add up to 50% of water and mix well. The pulverized product or the pulverized product mixed with water was sterilized at high temperature and high pressure (120 ° C., 1.5 atm, 20 minutes), cooled to room temperature, and then inoculated with 5% (v / v) of mushroom mycelium to 8 days at 25 ° C. Incubate. When using lyophilized powder (10% (w / w) of fruit or stem before drying), add water to each powder from 5 to 10% (w / v), mix well, sterilize mushroom mycelium as above. Inoculate and incubate.

Example 2 Mycelial Growth

Cactus fruit was cultivated without sterilization (120 ° C., 1.5 atm, 20 minutes) to cultivate the mushroom mycelium, and the change in growth (dry weight) over time is shown in Table 1.

Table 1 Mushroom Mycelial Growth

Example 3: Mushroom Mycelium Culture Extraction

After completion of mushroom mycelial culture, the mycelium and the culture solution are transferred to an extractor and extracted for 24 hours at 90 ° C., and then the filtrate is concentrated to a volume of 1/10 (80 ° C.) through a filtration step (particle filtration of 1 μm or more). The mushroom mycelium culture concentrate is dried. Drying is carried out by a method such as lyophilization, spray drying, hot air drying, and in this embodiment was dried by lyophilization. About 120 mg of extract was obtained from 1 L of the mushroom mycelium and culture mixture.

Example 4: Antioxidant Assay

An antioxidant assay was performed to see if there was an antioxidant effect using the extract obtained in Example 3.

Antioxidant assay was performed using DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical scavenging activity. After mixing 1 ml of DPPH solution (1.5 × 10 ^-4 M in EtOH) and 0.1 ml of sample, it is left to stand for 30 minutes and the degree of light absorption at 517 nm is investigated.

A mushroom mycelium culture extract (Example 3), which was cultured using the culture medium of the mushroom mycelium obtained as a control medium, was cultured using the palm cactus fruit as a medium. It was set as an experiment sphere. Antioxidant assay by DPPH radical scavenging activity showed about 60% increase in antioxidant activity in the experimental group. This was judged to be due to the components with antioxidant effects contained in palm cactus fruit.

Example 5: Preparation of Tablets

10 g of active ingredients

70 g of lactose

15 g of crystalline cellulose

5 g of magnesium stearate

Total amount 100 g

The tablets were prepared by direct tableting method after finely mixing the ingredients listed above. The total amount of each tablet is 100 mg, of which the active ingredient content is 10 mg.

Example 6 Preparation of Powder

10 g of active ingredients

50 g of corn starch

40 g of carboxy cellulose

Total amount 100 g

A powder was prepared by crushing and mixing the ingredients listed above. 100 mg of powder was added to the hard capsule 9 times to prepare a capsule.

As described above, the present invention by culturing the mushroom mycelium without special additives using the palm cactus, it is possible to mass culture of situation mushroom mycelium, Ganoderma lucidum mycelium, shiitake mushroom mycelium, fingerling mushroom mycelium, larch fungus mushroom mycelium and the like. , Mycelia and culture after cultivation is more excellent in terms of functionality than when cultured using a normal medium, it has the effect of further expanding the use range of mushroom mycelium and culture medium using the palm cactus medium.

In addition, the present invention greatly improved the inefficiency, such as the purchase of the whole or part of the medium from foreign and other regions for cultivating mushroom mycelium, so that it is produced in the region and distributed throughout the year and can be easily purchased, further contributing to the activation of the local economy. Has the effect.

Claims (9)

In the production of a medium for liquid culture of mushroom mycelium, Mushroom mycelium medium production method characterized in that to obtain the mushroom mycelium and the culture medium using the fruit, stem or powder of the palm cactus In liquid culture of mushroom mycelium, Mushroom mycelium is used as a strain, and the mixture is mixed well with 0-50% of water to the palm cactus fruit or stem pulverized powder, and then sterilized by high temperature and high pressure (120 ° C., 1.5 atm, 20 minutes). Inoculate% (v / v) and incubate for 6-8 days at 25 ~ 30 ℃. When using powders such as palm cactus fruit or stem as a medium, add 5 ~ 10% (w / v) of water to each powder Mushroom mycelium culturing method of culturing mushroom mycelium as above The method of claim 2, Mushroom mycelium cultured using palm cactus and its culture The method according to claim 2 and 3, Mushroom mycelium obtained by palm cactus as a medium and powder production method of mycelium and culture solution obtained by warm extraction, filtration, concentration, lyophilization, etc. The method according to claim 2 and 3, Mushroom mycelium obtained by palm cactus as a medium and extract method of mycelium and culture medium obtained by heating extraction, filtration and concentration of the culture medium The method according to claim 4 and 5, Method for producing functional foods and raw materials such as functional capsules, tablets, granules and the like containing mushroom mycelium prepared by adding the obtained mycelium, culture powder or extract in an amount of 0.1 to 70% (w / w) The method according to claim 4 and 5, Method for producing cookies, biscuits, gum, candy, bread, beverages, etc. containing mushroom mycelium prepared by adding the obtained mycelium, culture powder or extract in an amount of 0.05 to 10% (w / w) The method according to claim 2, 3, 4, 5, A method for producing fish and livestock feed containing mushroom mycelium prepared by adding the obtained mycelium and culture medium or powder and extract thereof in an amount of 0.1 to 20% (w / w) The method according to claim 2 and 3, Mushroom mycelium cultured using palm cactus, characterized in that the mushroom mycelium is used as a spawn for forming the mushroom fruiting body