KR101888555B1 - Composition comprising dried mixture of red shale and herbal medicine for immune enhancement - Google Patents

Abstract

The present invention relates to a composition for enhancing immunity comprising a herbal medicine and a red shale mixed preparation, wherein the composition is excellent in antibody forming ability even when the immunity is suppressed or lowered, and can be used for various purposes such as cold, cancer, infectious diseases, Can be usefully used as a composition for enhancing immunity in diseases.

Description

[0001] The present invention relates to an immunoconjugate composition comprising a mixture of a herbal medicine and a red shale,

The present invention relates to an immunoconjugate composition containing mixed dry matter of a herbal medicine and a red shale.

It is already known that immune function defends the living body from various pathogens. Vaccination and antitoxin use methods are used as methods for preventing and treating diseases using such immune function . In addition, as the mechanisms of action of the immune function are known, attempts have been made to directly use immune modulating substances capable of regulating immune function. Such immune modulating substances stimulate immune cells nonspecifically or specifically, Or enhancing the body's ability to defend the body from disease factors.

However, most of the above-mentioned immunomodulators are chemically synthesized substances, and some adverse effects or toxicity are partially found, and thus there are some restrictions on the application to actual living bodies. Recently, studies on immunomodulatory substances have been carried out through the validation of efficacies such as active ingredients extracted from natural or non - toxic food materials and conventional herbal medicine. As researches on physiologically active substances that enhance biological control and biological defense systems from natural products have been actively carried out, natural products are now being put into practical use as therapeutic agents or health supplement foods for alternative medicine.

Pueraria lobata (Willd.) Ohwi ( Pueraria lobata (Willd.) Ohwi) and Pueraria thomsonii Benth .: Powder ( Pueraria lobata (Willd.) Ohwi)葛) roots are used. Changgeun sweat, fever fever, high fever, cure headache and stop the thirst. It is used for digestion, headache, anemia, dysentery, abdominal pain, dryness, cold, vomiting and bleeding of wife. It blooms raw roots and sucks and drinks. The fruit of the 칡 is called the gag, and it is effective in the diarrhea. The 칡 flower which is a 칡 flower has a unique sweetness, and it releases the digestion and strengthens the intestines. 칡 powder is called galmium to stop the thirst, to see the feces and to see the children, fever is sick and the end is burning. Diabetes, dysentery, colitis, and malignant tumors.

Eastern prickly pear is the cactus and plant and is called the Opuntioideae cactus (Opuntia genus). It is the most common type with the stem being flat like a palm. Among the 'Opuntia' cacti native to Korea, it is often called the cactus, which grows in Jeju Island, as a cactus. The fleshy perennial plant is fleshy, like a typical cactus, and several places are constricted like a knot, which is called a cicada. There are many kinds of shapes such as cylindrical shape, ball shape, and elliptical shape, and they are classified according to their shapes. There is a vase in this passage and a vascular bundle near the epidermis. Leaves running on the larvae look like needles and grow up as flesh and fall off. The cotyledon is a bamboo (an apparatus with a blade used to cut the bamboo), a triangle or a triangle, and the flesh and bamboo (deformed leaves) are different from each other. It is possible to winter at minus 20 ℃. The shape of the stem is similar to that of the palm and is also called the palm cactus.

Salicornia herbacea ( Salicornia herbacea ) is an annual herbaceous herbaceous plant of the dicotyledonous plant-centered mackerel. It is officially called Tungtungmadi, but locally called Hamcho. It grows in tidal flats where the waters are well and relatively firm. It is 10 ~ 30cm in height. The stem is fleshy and has a cylindrical shape. The branches are opposite to each other, and the degenerated scaly leaves are running against each other, and the nodes bulge out, resulting in the name Tungtungmadi. The flowers bloom in August to September in green, and they run three in the concave part between the upper nodes of the branches. The horns are chubby squares with one to two stamens and one pistil. Ovaries are egg-shaped and two styles are long. Fruit is flat ovate, flat ovate, ripened in October, covered with hyssop, and contains black seeds. The entire abandon grows green, but in autumn it becomes reddish purple. It is distributed all over the west coast and south coast of Korea. Hamcho has long been eaten as a food for a long time. Cut the stem and boil the soup, or grind it and knead it together in flour. It is known as a health food because it is rich in minerals. In recent years, they have been cultivated on large farms as well as harvested from natural conditions.

Onion ( Allium cepa ) is a biennial plant with a monocotyledonous lily lily, and is presumed to be originated in West Asia or the Mediterranean coast. The cultivation history is so long that there are records that ancient Egyptian burial mounds around 3000 BC fed garlic and onion to pyramid workers, and grew in Greece from the 7th to 8th centuries BC. Scale stem is somewhat different according to varieties, but it has a flat round or round shape. It has thin purple color (semitransparent like translucent film) on its surface. Its inner scales are thick, This is fascinating. Leaves are hollow cylinders, dark green, dry when flowers are bloomed, and sculpted at the bottom. The flower blooms in September, and the flower stalk which comes out from the leaf forms a mountain inflorescence, and many numbers are round like a ball. The flower stalks are cylindrical, 50 ~ 100cm in height, bloom at the bottom, and 2 ~ 3 leaves under it. The horns are divided into 6 pieces, and the split pieces are basically shaped like inverted eggs and spread horizontally. There are six stamens, of which there are three on each side under the operating table, and one pistil. The varieties are divided into a round shape of the stem of the scales, a flat round shape, a red color of the scales stem, a yellow one, and a white one. It is also divided into Danyang and Spicy Onion depending on the taste. Generally, edible onion varieties are four varieties of yellow onion, red onion, white onion and small onion. The harvest is mainly from June to July when the leaves fall down and have a little green color. Some of the leaves of the scales are picked before the leaves are edited. Onion is mainly used for edible scales. The unique odor from scales is due to compounds such as disulfide, allyl sulfide. It physiologically promotes the secretion of digestive juices and has effects such as excitement, sweating, and diuretic. In addition, the scales stem contains various vitamins, minerals such as calcium and phosphorus, and has the action to remove harmful substances in the blood. Scale stem is often used in salads, soups, and meat dishes, and is used as a spice for various dishes. The leaf contains 5,000 IU of vitamin A, 45 mg of vitamin C, 80 mg of calcium, 24 mg of magnesium and 220 mg of potassium in 100 g of leaf.

Rice bran is also called rice bran. It is a fine bruch which is separated from the process of removing rice husks from rice and then turning brown rice into white rice.

Shale is a type of mudstone, also called shale. The shale has a property of thinning in a certain direction, which is a primary one due to the arrangement of particles in parallel with stratification or foliage. In contrast, it is said to be slate cancer that the secondary release surface is formed by pressure after sedimentation. Shale is divided into black shale and red shale depending on the color, and it is classified into corrosion shale (also called humic shale [humus shale], humic acid is abundant) according to the contained ingredients.

The black shale is abundant in animal organic matter, and it is black because it is hardly oxidized compared to the red shale. Black shale is mainly distributed in areas rich in crude oil reserves. Corrosion shales are abundant in vegetable organic matter because they are formed by hardening of soils deposited in mountains or fields. Corrosion shale is often used as a fertilizer additive.

The red shale is formed by hardening the soil deposited from the sea, and the amount of organic matter is small and the oxidation progresses to a red color. The shale in Korea is mainly red shale. The red shale is a porous fine powder and has very strong adsorption power and is known to have a surface area as large as about 20,000 times as large as the surface area of activated carbon. Therefore, studies have been made to adsorb harmful bacteria such as Escherichia coli, which parasitize in the intestines of the livestock, and heavy metals such as mercury or cadmium by such strong adsorption power. It is also known that red shale contains many more minerals than elvan. Minerals are minerals of abiotic matter, but minerals as biomaterials are mainly called mineral nutrients, mineral nutrients, and mineral elements. The elemental composition of inorganic nutrients is about 20, mainly Ca, Mg, P, Na, K, CI, which accounts for more than a dozen percent of the total. Other important trace elements are Cu Zn, I, Mn, and Co. In addition to the above, F, Mo, Se, Cr, Si, V, and Sn are indispensable for living organisms (Nursing Science Dictionary, March 1, 1996, Korean Dictionary Researcher). The mineral contained in the red shale is composed mainly of SiO ₂ , Al ₂ , O ₃ , MgO, K ₂ O, Fe ₂ O ₃ , CaO, TiO ₂ , Na ₂ O, P ₂ O ₅ and MnO. Mineral elements are abundant (Kwak, Sung Min, 2013)

The inventors of the present invention have found that a composition containing a red shale, a mixture of various herbicides and a saccharide as described above, while examining the efficacy of a mineral preparation, a natural product, or a conventional herbal preparation in the process of searching for a substance capable of exhibiting an immunomodulatory effect, The present invention has been completed.

Korean Laid-Open Patent Publication No. 2013-0039309 and Korean Laid-Open Patent Application No. 2012-0090582 disclose that a composition containing various herbal medicines can be used as a food or diet product having an immunity enhancing effect, The powder is not contained, and the content ratio of the herbal medicine is also different from that of the present invention. Korean Patent No. 1059280 discloses a composition for enhancing immunity containing Puerariae Radix extract. Korean Patent No. 897488 discloses a red shale-containing livestock feed composition for enhancing the immunity of animals. However, Japanese Patent No. 1059280 and Korean Patent No. 897488 do not disclose that it is possible to use milky ginseng, green tea, onion, rice or the like, so that it can be said that the invention is different from the present invention.

Korean Patent Laid-Open Publication No. 2013-0039309 (a food composition comprising a complex active substance of garlic and egg yolk as a health-improving functional medicinal agent and a preparation method thereof, published on Mar. 19, 2013) Korea Patent Publication No. 2012-0090582 (Diet composition using useful microorganisms and natural products, disclosed on Aug. 17, 2012) Korean Patent No. 1059280 (composition for enhancing immunity containing Puerariae Radix extract, registered on August 18, 2011) Korean Registered Patent No. 897488 (Red shale-containing livestock feed composition, registered on May 5, 2009)

Kwak, Sung - Min, Engineering Characteristics of Red and Black Shale in Daegu Area, Master Thesis, Graduate School, Kyungpook National University, 2013.

It is an object of the present invention to provide an immunostimulatory composition containing a mixed dry product of a herbal medicine and a red shale.

The present invention relates to a mixed dry matter of a herb medicine and a red shale,

(i) herbal medicines, including Puerariae radix, millennium, green onion, onion and rice bran; (ii) a red shale having 95% (w / w) or more of particles having a size of 200 to 1500 mesh; And (iii) a saccharide; and (iii) a saccharide.

(I) 100 parts by weight of herbal medicine including Puerariae radix, Chenille spp., Green tea, onion and rice bran; (ii) 7 to 15 parts by weight of a red shale in which particles having a size of 200 to 1500 mesh are distributed over 95% (w / w); And (iii) 10 to 30 parts by weight of saccharides.

The medicinal herb may be selected from the group consisting of Puerariae radix, Chenille seeds, green tea, onion, and rice bran at a weight ratio of 1: 0.5~3: 0.5~3: 0.5~3: 0.5~3.

In addition, the above-mentioned medicaments may be used in a variety of ways, such as a main door, a chimpanzee, a celestial gong, a gugija, a bokbunja, a coriander oil, a casualty, a garlic, Bamboo shoots, burdock, dandelion, taurine, shiitake mushroom, hinoki, yulmu, horsetail, gold eunhwaseun, white mugwort, lotus leaf, mulberry leaf, flaxseed, flaxseed, kelp, green tea, Sugar fermentation broth of yeast extract, lactic acid bacteria, vitamins and plants (fermentation enzyme (fermentation enzyme), fermentation enzyme (fermentation enzyme), fermentation enzyme ) May be added to one or more herbal medicine.

Further, the present invention relates to a mixed dry matter of a herbal medicine and a red shale, comprising: (i) a herbal medicine comprising Puerariae radix, millennium, green onion, onion and rice bran; (ii) a red shale having 95% (w / w) or more of particles having a size of 200 to 1500 mesh; And (iii) a saccharide; and (iii) a saccharide.

In another aspect, the present invention provides a mixed dry matter of a herbal medicine and a red shale, comprising: (i) a herbal medicine comprising Puerariae, Thunb. (ii) a red shale having 95% (w / w) or more of particles having a size of 200 to 1500 mesh; And (iii) a saccharide; and an animal feed additive for immunity-enhancing animal containing the dry matter.

The present invention also relates to

(Step 1) Firstly crushing the red shale ore with a jor crusher and secondly crushing it with a hammer crusher to prepare a red shale having a size of 1 to 30 mm;

(2 steps) The second shredded red shale was prepared as a third shredded red shale through a third screen, and the third shredded red shale was subjected to a grinding process using a grinder selected from a ball mill, a roller mill, Preparing a fourth order pulverized red shale having a particle size of ~ 200 mesh;

(Step 3) The fourth shredded red shale is subjected to a fifth grinding process using a Raymond mill to prepare a red shale having a particle size range of 200 to 1500 mesh and distributed over 95%. And

(4 steps) 100 parts by weight of herbal medicine including Puerariae radix, Puerariae radix, green onion, onion and rice bran, and particles having a size of 200 to 1500 mesh produced in the above 3 steps are distributed over 95% (w / w) 7 to 15 parts by weight of red shale and 10 to 30 parts by weight of saccharide, followed by drying;

And a method for producing a mixed dry product of a herbal medicine and a red shale.

Hereinafter, the present invention will be described in detail.

The present invention relates to a mixed dry matter of the herbal medicine and a red shale, which comprises: (i) a herbal medicine comprising Puerariae radix, Chenille spp., Green onion, onion and rice bran; (ii) a red shale having 95% (w / w) or more of particles having a size of 200 to 1500 mesh; And (iii) saccharides. In detail, the composition for immunomodulation comprises, in terms of 100 parts by weight of a herbal medicine comprising Puerariae radix, Chenille spp., Green onion, onion and rice bran, 200 7 to 15 parts by weight of a red shale having a particle size of ~ 1500 mesh distributed over 95% (w / w) and 10 to 30 parts by weight of saccharides, If the amount of the red shale is less than 7 parts by weight or more than 15 parts by weight, the immunostimulating effect may be lowered. The content of the saccharide is not particularly limited, but it is appropriate that the saccharide is 10 to 30 parts by weight based on 100 parts by weight of the crude drug to be produced as a mixed dry product. When the sugar is less than 10 parts by weight, it is difficult to mix. When the sugar is more than 30 parts by weight, the drying time is longer.

The herbal medicine may be a mixture of Puerariae radix, Puerariae radix, Onion radish, Onion radish, and Rice gruel. Can be mixed in a weight ratio. At this time, if the weight ratio is out of the above range or if at least one kind of herbal medicine is not contained, the immunity enhancing effect may be lowered.

Preferably, the herbal medicine is made of powder, and the particle size of the powder is not particularly limited, but is preferably 95% or more in a particle size range of 200 to 1500 mesh, similar to a red shale.

The red shale may have a particle size of 200 to 2000 mesh. In addition, the feed composition preferably contains a red shale having a particle size range of 200 to 1,500 mesh and more than 90% of the red shale, and a red shale having a particle size range of 200 to 1,500 mesh desirable.

Prior to entering the primary grinding process for powdering the red sail, washing and drying procedures for the red shale gemstones may be included. The manufacturing process of the red shale is disclosed in Korean Patent No. 897488 of the present inventors. The red shale is about the silica (SiO ₂₎ about 58.1% by weight, alumina (Al ₂ O ₃₎ to about 14.2% by weight, oxidizing iron (Fe ₂ O ₃₎ to about 4.55 wt%, calcium oxide (CaO) 7.55% by weight of magnesium oxide, about 1.58% by weight of magnesium oxide, about 7.84% by weight of ignition loss (IgIoSS), about 4.06% by weight of potassium oxide (K ₂ O) and about 2.12% by weight of other minor components. The apparatus for crushing the red shale such as the jaw crusher, the hammer crusher, the ball mill, the roller mill, the grinder, and the raymond wheat used for pulverizing the red sail may be changed to another crushing apparatus of a similar purpose commonly used .

The kind of the saccharide is not particularly limited, but it is preferable to use at least one saccharide selected from the group consisting of honey, oligosaccharide, agave syrup, maple syrup and 40 to 60% (w / w) sugar syrup.

In addition, the above-mentioned medicaments may include, but are not limited to, a yambi gate, a chimpanzee, a chrysanthemum, a chrysanthemum, a bokbunja, a marine oil, a casualty, a garlic, a tosaja, , Bamboo shoots, dandelion, taurine, shiitake mushrooms, hinoki, yulmu, gimhwal, ginhwada (bamboo shoots), bamboo shoots, Sugar fermentation solution of lactic acid bacteria, vitamins and vegetable matter, lactic acid bacteria, yeast extract, lactic acid bacteria, vitamins and plants (Fermentation enzyme), and they are preferably added in the form of a dried powder, and it is preferable that they are added in an amount of 100 parts by weight of a herbal medicine comprising Puerariae radix, Chenille spp., Green onion, onion and rice bran Based on 10-100 Weight parts are preferably added. The sugar fermentation broth of the above plant is obtained by mixing various plants with one or more saccharides selected from the group consisting of honey, oligosaccharide, agave syrup, maple syrup and sugar at a weight ratio of 1: 0.5 ~ 2, Refers to the liquid phase obtained by removing the mash from the fermentation product.

On the other hand, when preparing the mixed dry matter of the herbal medicine and the red shale, the medicinal herb, the red shale and the saccharide may be manufactured into a ring form using a ventilator commonly used in the manufacture of rings and dried. The size of the ring is not limited, but it is preferably from 1 to 20 mm, more preferably from 2 to 10 mm, and most preferably from 3 to 5 mm, depending on the use and the age of the subject. The drying temperature and time of the mixed dried material are not particularly limited, but it is preferable to dry at 30 to 50 ° C for 12 to 30 hours.

In addition, the present invention provides a pharmaceutical composition for enhancing immunity, which comprises a mixed dry matter of the herbal medicine and the red shale. The pharmaceutical composition may be used in oral formulations such as powders, granules, tablets, pills, capsules and the like according to conventional methods. Examples of carriers, excipients and diluents that can be contained in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose , Methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient, such as starch, calcium carbonate, sucrose or lactose, Gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used.

The dosage of the pharmaceutical composition of the present invention will depend on the age, sex, body weight of the subject to be treated, the particular disease or condition to be treated, the severity of the disease or condition, the route of administration and the judgment of the prescriber. Dosage determinations based on these factors are within the level of ordinary skill in the art and generally the dosage ranges from 0.01 mg / kg / day to approximately 2000 mg / kg / day. A more preferable dosage is 1 mg / kg / day to 500 mg / kg / day. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

The pharmaceutical composition of the present invention is most preferably orally administered to mammals such as rats, livestock, humans, and the like.

The present invention also provides a health functional food for immunity enhancement comprising a mixed dry product of the herbal medicine and the red shale and a food acceptable food supplementary additive. The mixed dry matter of the herbal medicine and the red shale may be added in an amount of 0.001 to 100% by weight to the health functional food of the present invention. The health functional food of the present invention can be produced in the form of tablets, capsules, pills, powders, and the like. The health functional foods of the present invention include, for example, various foods, beverages, gums, tea, and vitamin complexes.

The present invention also provides an animal feed additive for immunity enhancement containing mixed dry matter of herbal medicine and red shale. The mixed dry matter of the herbal medicine and the red shale may be used as an animal feed additive for an immune enhancing animal to be added to a nutritional supplemented feed for livestock, and the nutrient supplemented feed may include various nutritional supplemented feeds according to the kind of livestock. It is preferable that 1000 to 3000 parts by weight of the nutrition compound feed is mixed on the basis of 100 parts by weight of the feed additive. When the mixture of the herbal medicine and the red shale is mixed with the nutrient mixture feed, it is preferable that the dry mixed material is added in powder form.

The present invention relates to a composition for enhancing immunity comprising a herbal medicine and a red shale mixed preparation, wherein the composition enriches the pharmacological ingredient of the herbal medicine and the inorganic ingredient of the red shale, so that even when the immunity is suppressed or decreased, And is useful as a composition for enhancing immunity in various diseases such as cold, cancer, infectious diseases, vascular diseases, geriatric diseases, and the like.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flow chart showing a process for producing a composition containing mixed dry matter of a herbal medicine and a red shale of the present invention. FIG.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, the intention is to provide an exhaustive, complete, and complete disclosure of the principles of the invention to those skilled in the art.

<Example 1> Preparation of herbal medicine>

The dried powder was dried to a moisture content of 15% or less at 40 ° C using a hot-air drier, and then pulverized with a conventional herbicide pulverizer to give 95% or more of the powder in a particle size range of 200 to 1500 mesh Dried powder, millet seed powder, green tea powder, onion powder and rice bran powder were prepared.

&Lt; Example 2: Preparation of red shale >

Silica (SiO ₂₎ 58.1% by weight, alumina (Al ₂ O ₃₎ 14.2% by weight, oxidizing iron (Fe ₂ O ₃₎ 4.55% by weight, calcium (CaO) 7.55% by weight of oxide, magnesium (MgO) 1.58% by weight of oxidation , 7.84% by weight of IgIoSS, 4.06% by weight of potassium oxide (K ₂ O) and 2.12% by weight of other minor components were first crushed with a jor crusher and then crushed with a hammer crusher to obtain 2 The mixture was crushed and made into red shale lumps 1 to 30 mm in size. The second shredded red shale mass is made into a third shredded red shale through a third screen, and the third shredded red shale is sequentially pulverized with a ball mill, a roller mill, and a grinder, A fourth shredded red shale with particle size was prepared. The fourth shredded red shale was subjected to a fifth milling process using a raymond mill to obtain a red shale having a particle size of 200 to 2000 mesh and a red shale having a red color distribution of more than 95% Shale.

&Lt; Example 3: Preparation of crude drug mixture >

The granular powder, the granular powder, the green tea powder, the onion powder and the rice bran powder of Example 1 were mixed under the conditions shown in Table 1 below to prepare a herbal mixture.

Condition Puerariae powder
(g) Millennium powder
(g) Green tea powder
(g) Onion powder
(g) Rice bran powder
(g) total
(g) Example 3-1 100 50 300 50 300 800 Example 3-2 100 300 50 300 50 800 Example 3-3 100 150 200 150 200 800 Example 3-4 70 210 210 140 170 800 Example 3-5 150 150 150 150 200 800

Example 4 Preparation of a Composition Containing a Dry Mixture of Herb Medicine, Red Shale and Saccharides [

The herbal mixture of Example 3, the red shale of Example 2 and the oligosaccharide, which are sugars, were prepared under the conditions shown in Table 2 below, and the above materials were mixed in a conventional ventilator for producing a ring, Sized ring shape and dried by hot air at 40 캜 for 24 hours to prepare a composition containing the mixed dried product of red shale, herbal medicine and saccharides of the present invention.

Condition Example 3-1
The crude drug mixture (g) In Example 3-2
The crude drug mixture (g) Example 3-3
The crude drug mixture (g) Example 3-4
The crude drug mixture (g) In Examples 3-5
The crude drug mixture (g) Example
2 red shale (g) The oligosaccharide (g) Total (g) Example 4-1 100 0 0 0 0 10 15 125 Example 4-2 100 0 0 0 0 7 18 125 Example 4-3 100 0 0 0 0 15 10 125 Example 4-4 0 100 0 0 0 10 15 125 Example 4-5 0 0 100 0 0 10 15 125 Examples 4-6 0 0 0 100 0 10 15 125 Examples 4-7 0 0 0 0 100 10 15 125

 < Comparative Example  1. Preparation of comparative herbal mixtures>

The granular powder of Example 1, the granular powder, the powder of Hanchon, the powder of onion, and the powder of rice bran were mixed under the conditions shown in Table 3 below to prepare a herbal mixture.

Condition Puerariae powder
(g) Millennium powder
(g) Green tea powder
(g) Onion powder
(g) Rice bran powder
(g) total
(g) Comparative Example 1-1 100 240 400 30 30 800 Comparative Example 1-2 200 0 300 300 0 800 Comparative Example 1-3 100 300 0 50 350 800 Comparative Example 1-4 0 300 300 0 200 800 Comparative Example 1-5 200 0 300 300 0 800 Comparative Example 1-6 0 200 200 200 200 800

< Comparative Example  2. Comparative herbs, Red Shale  And a sugar dry mixed material &

The same procedure as in Example 4 was carried out to prepare a ring composition, and the mixing weight of each material was prepared by referring to Table 4 below.

Condition The crude drug mixture (g) of Comparative Example 1-1 The crude drug mixture (g) of Comparative Example 1-2 The crude drug mixture (g) of Example 3-1 The red shale (g) of Example 2 The oligosaccharide (g) Total (g) Comparative Example 2-1 100 0 0 10 15 125 Comparative Example 2-2 0 100 0 10 15 125 Comparative Example 2-3 0 0 55 55 15 125 Comparative Example 2-4 0 0 0 110 15 125 Comparative Example 2-5 0 0 110 0 15 125

EXPERIMENTAL EXAMPLE 1 Confirmation of Immune Enhancement Effect (1)

To investigate the effect of the composition of Example 3 and Comparative Example 1 on the immune system, the effect of IgM and IgG antibodies on male Balb / c mice of 12 weeks old and 78 weeks old was investigated.

Mice were adapted and used under constant conditions (temperature: 20 ± 2 ℃, humidity: 40 ~ 60%) after pre-sale. The mice were divided into 5 mice per group, and only the distilled water was administered to the control group. The herbal mixture (100 mg / kg each) of Example 3 and Comparative Example 1 was orally administered to each group for 14 days, And dissolved in 0.5 ml of distilled water.

The immunosuppressive agents were administered intraperitoneally to the mice for 2 weeks before the administration of glucocorticoid (80 mg / kg, ip) once a week for 2 weeks before 12 weeks of age, .

MA-BSA conjugated with bovine serum albumin (BSA) was used as a hapten MA for methamphetamine, which was used as a Freund's complete adjuvant and incomplete adjuvant. Were mixed and immunized in the abdominal cavity of each mouse. This work was performed after 4 days of administration of the sample (herbal drug mixture of Example 3 and Comparative Example 1). After the antigen administration, the mice were again administered daily for 7 days, and the antigen was again mixed with the Freund's adjuvant and re-immunized. After oral administration of the sample for 3 days, the blood was collected again. The collected blood was allowed to stand at room temperature for 30 minutes and the serum was separated and used for the experiment (oral administration for 14 days).

The amount of immunoglobulin in serum was measured by ELISA (enzyme linked immunosorbent assay) method. MA-BSA was adjusted to 2 μg / ml with a coating buffer (pH 9.0), added with 50 μl of each well of an ELISA plate, reacted for 2 hours, washed with plate, (biotinylated rabbit anti-mouse IgG2b) or biotinylated rabbit anti-mouse IgG2b (biotinylated rabbit anti-mouse IgM), which specifically binds to the heavy chain, Lt; / RTI &gt; After incubation with peroxidase-conjugated streptavidin for 1 hour, the absorbance was measured at 492 nm using o-phenylenediamine as a substrate. The results were as follows: 12-week no-treatment The results are shown in Table 5 below.

Oral administration
Composition condition
IgM antibody production (fold) IgG antibody production (fold)
12 weeks 12 weeks +
Glucocorticoid
78 weeks
12 weeks 12 weeks +
Glucocorticoid
78 weeks Example 3-1 1.38 0.83 0.93 1.44 0.83 0.84 Example 3-2 1.42 0.74 0.86 1.24 0.74 0.98 Example 3-3 1.39 0.95 0.95 1.43 0.95 0.96 Example 3-4 1.29 0.82 0.94 1.35 0.86 0.85 Example 3-5 1.34 0.83 0.83 1.23 0.87 0.84 Comparative Example 1-1 1.12 0.55 0.77 1.13 0.62 0.55 Comparative Example 1-2 1.17 0.49 0.66 1.02 0.65 0.64 Comparative Example 1-3 1.12 0.51 0.65 1.15 0.52 0.63 Comparative Example 1-4 1.12 0.57 0.58 1.23 0.73 0.52 Comparative Example 1-5 1.18 0.62 0.68 1.25 0.64 0.63 Control group 1.00 0.44 0.65 1.00 0.33 0.56

Referring to Table 5, it can be seen that the herbal medicine mixture of Example 3 of the present invention is superior in the amount of antibody production as compared to the control group of the untreated control group or the herbal medicine mixture of Comparative Example 1. In particular, in the case of 12-week-old mice in which glucocorticoids were administered to suppress immunity and 78-week-old mice that were immune to aging, immunity was increased up to a level similar to that of general 12-week-old mice after administration of the composition of the present invention.

EXPERIMENTAL EXAMPLE 2. Confirmation of Immune Enhancement Effect (2)

In order to confirm the effect of enhancing the immunity when the red shale was mixed with the herbal composition, the amount of the antibody produced by the method of Experimental Example 1 was compared with that of the cyclic composition of Example 4 and Comparative Example 2, .

Oral administration
Composition condition
IgM antibody production (fold) IgG antibody production (fold)
12 weeks 12 weeks +
Glucocorticoid
78 weeks
12 weeks 12 weeks +
Glucocorticoid
78 weeks Example 4-1 1.63 1.58 1.69 1.77 1.60 1.64 Example 4-2 1.74 1.47 1.56 1.64 1.52 1.53 Example 4-3 1.65 1.36 1.57 1.65 1.55 1.52 Example 4-4 1.66 1.55 1.40 1.56 1.63 1.71 Example 4-5 1.67 1.34 1.62 1.77 1.42 1.47 Examples 4-6 1.53 1.43 1.53 1.63 1.59 1.66 Examples 4-7 1.74 1.66 1.44 1.64 1.73 1.59 Comparative Example 2-1 1.25 0.92 1.12 1.11 0.82 0.85 Comparative Example 2-2 1.27 0.83 1.20 1.12 0.78 0.94 Comparative Example 2-3 1.14 0.92 0.94 1.20 0.86 0.90 Comparative Example 2-4 1.10 0.89 0.75 1.13 0.62 0.63 Comparative Example 2-5 1.05 0.92 0.72 1.15 0.84 0.83 Control group 1.00 0.40 0.62 1.00 0.35 0.51

Referring to Table 6, the composition of Example 4 (a mixture of 7-15 parts by weight of red shale and 10-30 parts by weight of oligosaccharide based on 100 parts by weight of the crude drug mixture of Example 3) , It can be seen that there is a remarkable effect of enhancing the immune system as compared with the case of deviating from the scope of manufacturing the composition for immunostaining according to the present invention. In particular, the immunity of 12-week-old mice or 78-week-old aged mice in which the immunosuppressive effect was suppressed by administration of glucocorticoid was significantly increased to a level similar to that of the 12-week-old mice to which the composition of Example 4 was administered. The immunity is increased by 40 to 80% compared to the control 12-week-old mouse. This effect is expected to be the enhancing effect of the addition of the minerals contained in the red shale to the immunostimulability of the herbal mixture of Example 3.

On the other hand, even if the composition of Comparative Example 2 contains the herbal mixture of Comparative Example 1 or contains the herbal mixture of Example 1, the mixing ratio of the red shale powder is different from that of the composition of the present invention. It can be seen that the reinforcing effect is very poor.

Thus, it can be seen that only the composition of the present invention is suitable for use as an immunomodulating composition.

<Experimental Example 3: Identification of disease resistance of chick>

Example 4 and Comparative Example 2 to screen the composition 100g of powdered nutritional formula feed were mixed to 2000g per 20 day-old Ross (Ross) after two weeks benefit in each group 10 rats in broiler chickens for Salmonella strain (Salmonella gallinarum ) were cultured. The number of strains was adjusted to be 1 x 10 ⁹ CFU / ml using saline solution, and then the solution was orally administered by 1 ml each. At this time, only the nutritional formula was fed to the control group. Thereafter, the survival rate of the chicken was confirmed while feeding the feed composition in the same manner for 15 days, and the results are shown in Table 7.

As shown in the results of Table 7, it was found that all the chicks survived despite the administration of the Salmonella strain in the group fed the composition of Example 4 of the present invention together with the nutritional formula, It can be seen that approximately 30-50% of chickens died during 15 days in the control group fed only with the nutritional formula feed or the nutrient mixture feed (in the same conditions, the chick fed only the nutrient mixture as the non-Salmonella strain, 100% survive). Accordingly, it can be seen that the compositions of the present invention have an effect of enhancing the immunity of chickens to which Salmonella strains are administered.

Claims (13)

(i) 100 parts by weight of herbal medicine mixed in a weight ratio of 1: 0.5 ~ 3: 0.5 ~ 3: 0.5 ~ 3: 0.5 ~ 3 of Puerariae radix;
(ii) 7 to 15 parts by weight of a red shale in which particles having a size of 200 to 1500 mesh are distributed over 95% (w / w); And
(iii) 10 to 30 parts by weight of saccharides;
Of the total weight of the composition. delete delete delete (i) 100 parts by weight of herbal medicine mixed in a weight ratio of 1: 0.5 ~ 3: 0.5 ~ 3: 0.5 ~ 3: 0.5 ~ 3 of Puerariae radix;
(ii) 7 to 15 parts by weight of a red shale in which particles having a size of 200 to 1500 mesh are distributed over 95% (w / w); And
(iii) 10 to 30 parts by weight of saccharides;
And a mixed dry matter of the present invention. delete delete delete (i) 100 parts by weight of herbal medicine mixed in a weight ratio of 1: 0.5 ~ 3: 0.5 ~ 3: 0.5 ~ 3: 0.5 ~ 3 of Puerariae radix;
(ii) 7 to 15 parts by weight of a red shale in which particles having a size of 200 to 1500 mesh are distributed over 95% (w / w); And
(iii) 10 to 30 parts by weight of saccharides;
Wherein the animal feed additive is an animal feed additive for immunity enhancement. delete delete delete (Step 1) Firstly crushing the red shale ore with a jor crusher and secondly crushing it with a hammer crusher to prepare a red shale having a size of 1 to 30 mm;
(2 steps) The second shredded red shale was prepared as a third shredded red shale through a third screen, and the third shredded red shale was subjected to a grinding process using a grinder selected from a ball mill, a roller mill, Preparing a fourth order pulverized red shale having a particle size of ~ 200 mesh;
(Step 3) The fourth shredded red shale is subjected to a fifth grinding process using a Raymond mill to prepare a red shale having a particle size range of 200 to 1500 mesh and distributed over 95%. And
100 parts by weight of herbal medicine mixed in a weight ratio of 1: 0.5 ~ 3: 0.5 ~ 3: 0.5 ~ 3: 0.5 ~ 3, 200 ~ 1500 7 to 15 parts by weight of a red shale having a mesh size of 95% (w / w) or more and 10 to 30 parts by weight of saccharides, followed by drying;
Wherein the mixed dry matter is a mixture of herbal medicine and red shale.

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