KR101109415B1 - A feed additives for prophylaxis and treatment of viral disease including newcastle disease and a feed composion - Google Patents

Abstract

PURPOSE: A feed additive for poultry feed having a virucidal effect to a newcastle disease virus, and a feed composition containing thereof are provided to improve the immunity of the poultry. CONSTITUTION: A feed additive for poultry feed is obtained by the following steps: cutting and crushing dried or frozen mixing ingredients, and mixing all the ingredients; dipping the mixed ingredients in water for 10-50 hours; extracting the ingredients with an extraction solvent; filtering and centrifugal separating the ingredients; and concentrating and freezing the obtained additive. The mixing ingredients include 4-8wt% of glycyrrhizae radix, 4-8wt% of mugwort, 5-9wt% of garlic, 5-9wt% of onion, 7-10wt% of atractylodes lancea, 7-10wt% of pueraria thunbergiana, 8-10wt% of polygonatum sibiricum, 8-10wt% of ginkgo nut, 11-15wt% of aloe vera, and 11-15wt% of canavalia gladiata seeds.

Description

A feed additives for prophylaxis and treatment of viral diseases including Newcastle disease and a feed composion

The present invention includes Newcastle disease of poultry The present invention relates to a feed additive and a feed composition having a prophylactic and therapeutic activity for viral diseases. Specifically, the present invention relates to a feed additive that is effective in preventing the occurrence of Newcastle disease in poultry and reducing the incidence or treating Newcastle disease. In addition, the present invention relates to a feed composition containing the feed additive.

Newcastle disease in poultry, including birds, is caused by paramyxovirdae paramyxovirus, and Newcastle disease is caused by the Newcastle-upon-Tyne region of Java and England in 1926. It is known to be the first to occur in algae.

The infection path of poultry Newcastle disease is caused by air or oral, including secretions, feces, respiratory excretion, and direct contact with infected bird bodies.The main symptoms include cough, dyspnea, anorexia, and yellow-white diarrhea. Green diarrhea, paralysis, convulsions, turning movements, head, wings, leg twists, air bladder turbidity, bleeding spots in the sac and intestine.

Newcastle disease in poultry occurs at any age, regardless of age, is a highly infectious, terrible first class infectious disease in poultry that causes 100% mortality when infected with unvaccinated chickens. Although an accrual announcement has been issued, the outbreak is not only on the rise, but is also occurring nationwide, causing serious damage to poultry farmers.

As a prior art related to the treatment and prevention of Newcastle disease of poultry, for example, Korean Patent Publication No. 10-2009-7012384, the treatment of Newcastle disease using extract obtained from turmeric, green tea, ginger, wasabi The present invention discloses a method and a method for suppressing incidence, and Korean Patent Laid-Open Publication No. 2002-0041118 discloses a treatment and prophylactic agent for Newcastle disease of poultry containing cetylpyridinium chloride. The therapeutic agent itself is not directly related to feed additives and feed compositions, and Korean Patent Publication No. 10-0743861 describes human diseases caused by viruses such as influenza A virus, Newcastle disease virus, chicken infectious bronchitis virus and avian pneumovirus. Therapeutic and prophylactic compositions are disclosed but these are human diseases. It relates to prevention and treatment.

The present invention includes Newcastle disease of poultry The purpose of the present invention is to provide a feed additive having a prophylactic and therapeutic activity for viral diseases and to provide a feed composition containing the feed additive, and more specifically, to include Newcastle disease of poultry. It has the purpose of providing a feed additive which effectively prevents and treats Newcastle disease of poultry, and has a high efficacy in enhancing the immunity of poultry while preventing and treating viral diseases. The purpose is to provide.

Newcastle disease of poultry for achieving the object of the present invention Ingredients of feed additives with the prevention and treatment of viral diseases include licorice (Glycyrrhiza uralensis, Licorice) 4 to 8% by weight, mugwort (Folium Artemisiae Argyi) 4 to 8% by weight, garlic (Allium sativum L.) 5 ~ 9% by weight, onion (Allium cepa L.) 5-9%, Atractylodis Rhizoma 7-10%, Pueraria thunbergiana Benth 7-10%, Astragalus membranaceus 8-10% %, 8-10 wt% ginkgo nut, 11-15 wt% aloe vera, and 11-15 wt% seed of Canavalia ensiformis.

In addition, it includes Newcastle disease of poultry according to the present invention The feed composition having a prophylactic and therapeutic activity for viral diseases consists of blending the feed additive in an amount of 0.1 to 55 parts by weight based on 100 parts by weight of a conventional poultry feed.

Licorice (Glycyrrhiza uralensis, Licorice) as a component of the feed additive according to the present invention harmonizes the toxicity of all drugs to make the drug appear well and communicates well all blood vessels and strengthens muscles and bones, and the pharmacological action is detoxification. Inhibits peptic ulcer along with action, diuretic and anti-inflammatory effects. The roots and roots of licorice contain triterpene system saponin and glycyrrhizin, and 2-glucuron acid of glycyrrhizin acid. Glycosides are a sweet component of licorice. This glycoside has no blood action, but glycyrrhetin acid has blood action, and glycyrrhetin acid is contained as 18α-glycirrhetic acid, uralene acid in the hydrolysis component of licorice. have.

The mugwort (Folium Artemisiae Argyi) has been used to treat tonic blood and diarrhea for a long time, and the leaflets contain 0.02% of essential oils, and the main essential oils are cineol 50%, alkaloids, and chuon (Thujone), Sesquiterpen, Sesquiterpen alcohol, Sesquiterpen alcohol, Adenine 0.02%, Choline 0.11%, Vitamin A, B, C, D and the like.

The garlic (Allium sativum L.) is rich in allicin, sulfur compounds, selenium, vitamins B, C, E, etc. In general, garlic contains about 1% of volatile essential oil, of which 60% Diallyl disulfide and the rest are allylpropyl disulfide, diallyl trisulfide, allicin and the like, mostly sulfur-containing compounds. Among them, allicin is combined with vitamin B1 to become an alliamine, which continuously shows the effects of vitamin B1 and helps absorption of vitamin B2 and vitamin C. In addition, it is an excellent anti-oxidant, antimicrobial and anti-viral effect, it inhibits the growth of various harmful disease factors bacteria, leads to the stability of beneficial bacteria total, and has excellent antimicrobial and anti-oxidant effects against diseases, and is excellent as an antibiotic replacement agent. In addition to bacteria has the effect of inhibiting the growth of viruses.

Onion is effective in gastrointestinal discomfort and enteritis, has a bactericidal action, inhibits plasma cholesterol rise, and decreases fibrin lytic activity.

Onions belong to the Liliaceae, which consists of more than 80% of the flavoids consisting of quercetin diglycoside, monoglycosiden and quercetin aglycone. It contains a small amount of special flavours such as dimethyl disulfide, diallyl-disulfide and diallyl thioether, thiosulfine acid salt and small amounts of malate. Roots, apertures, and leaves contain coumaric acid, caffeic acid, ferulic acid, and sinapic acid.

Atractylodis Rhizoma works to remove moist heat and to make the stomach more comfortable. Creation is another name for the shovel that makes diarrhea better off inexhaustible, and eliminates species and spears. There are about 1.5% essential oils, carotene, inulin, gum and alkaloid reactions, and outposts have saponin and coumarin reactions. The main components of the essential oil are sesquiterpene, atlactilone (C 15 H20 O, melting point 38 ℃, about 20%), furfural, β-Eudesmol, henesol, art Lactylol, isovaleric acid ester, atactic lactate potassium and the like. In addition, the essential oil component of the root of the Atractylodes Koreana Kitam (creation) is atlactylodine, and contains a small amount of acetyl atlactylodinol, atlactylodinol, and elemol. There is very little or no atlactilone, the main component of the essential oil.

Brown root (Pueraria thunbergiana Benth) is peeled and finely chopped root roots, soaked in brine or white rice and dried. The outer part is grayish white fibrous and tastes bitter. It prevents osteoporosis, has potential antiviral effects, helps diarrhea in the body, and has an excellent immunopotentiating effect in the prevention, inhibition and treatment of immunosuppression. The root component contains flavonoid compounds such as puerarin, daizein and daidzin, and saponin and starch such as soyasaponin. .

Astragalus membranaceus has an immune enhancing effect to enhance the phagocytosis of reticulum endothelial cells, effective for chronic bronchitis, bronchial asthma, allergic rhinitis, leukopenia is known to be effective. Its main ingredient contains 7,3-dihydroxy-4-methoxyisoflavone, a flavonoid compound, and formononetin, 9- 9-Dimethoxyptterocarpan (9-Dimethoxyptterocarpan), etc., Saponin (Astragaloside) as a component, D-glucose (D-glucose), D-fructose (D- fructose), sucrose and the like.

Ginkgo nut is known to have effects such as cough and expectoration, and is used for the treatment of seawater, asthma, oil wells, whitening of urine and frequent urine. Ganol, pectin, histidine, starch, protein, fat, and sugar contained in ginkgo are effective for pulmonary tuberculosis asthma, cough and sputum. Ginkgo kills the inflammation of respiratory function and suppresses the development of Mycobacterium tuberculosis. have. In addition, the bank has the effect of improving the brain vessels, such as nervous breakdown, systemic fatigue.

The aloe vera gel of aloe vera contains a small molecular weight immunomodulator (G1C2F1), which restores the function of UV-stretched skin Langerhans cells, and heals wounds, antitumor activity and Activate macrophages. It has antiviral, antibacterial, muscle relaxation, radiation defense, anti-inflammatory and immunopotentiating effects. Also, acemanan of Aloe vera is used as a vaccine and effective antigen adjuvant for avian virus disease. There is activity as a strong backing agent for viral disease vaccines such as Newcastle disease.

Canavalia ensiformis seeds are rich in nutrition because they contain amino acids such as urease, hemagglutinin, glucosidase canalin, and navarin, starches, proteins, oils, and vitamins A, B1, B2, B3, and C. Do. In particular, hemagglutinin is composed of various globulins such as concanavalin A, or concanavalin A is a lectin that binds to mannose / gurucose, which inhibits mouse lymphocyte lysis in vitro and activates macrophages. And promotes the removal of Candida albicans in tissues.

Including the Newcastle disease of poultry according to the present invention (Newcastle) Each of the ingredients of the feed additive having the prevention and treatment of viral diseases can be obtained from conventional herbal materials, which are usually grown or marketed, and are not particularly limited, and drying and powdering the raw materials of each of the above components It can be prepared as a feed additive by mixing in a blending ratio according to the present invention.

In addition, it includes Newcastle disease of poultry according to the present invention Another method for preparing a feed additive having a prophylactic and therapeutic activity for a viral disease can be prepared by combining extracts extracted from each component of the feed additive, as well as a complex herbal extract extracted from a mixture of the components of the feed additive. It can be prepared a feed additive, it will be described below a method for producing a feed additive of the present invention by a composite herbal extract.

First, the dried or frozen state of the licorice, mugwort, garlic, onion, creation, brown root, Astragalus, ginkgo, aloe vera, and soybean seed, which are the components of the feed additive, respectively, are finely sliced, and then the combination ratio according to the present invention. After mixing or immersing in water for 10 hours to 50 hours, preferably 20 hours to 30 hours, and then purified water (water), lower alcohol of 1 to 4 carbon atoms or purified water (water) and lower of 1 to 4 carbon atoms After extraction using a solvent selected from a mixed solvent of alcohol, the extract is filtered and centrifuged, concentrated and freeze-dried to produce a composite herbal extract, which is used as a feed additive according to the present invention.

The extraction method may use hot water extraction, cold extraction, reflux cooling extraction or ultrasonic extraction, and 1 to 20 times, preferably 5 to 10 times the weight of the material mixture, which is a mixture of the components of the feed additive. It is preferable to extract hot water at 10 ° C. to 200 ° C. for 10 minutes to 10 hours using water containing purified water as a solvent, and more preferably to extract hot water for 1 hour to 2 hours at a temperature of 50 ° C. to 150 ° C.

In addition, the centrifugation process is centrifuged twice at 1,000 rpm to 4,500 rpm at a temperature of -10 ° C. to 10 ° C. using a centrifuge, and more preferably at 2,000 rpm to 4,000 rpm at 4 ° C.

The feed additive according to the present invention can be administered as a feed compounded in a conventional poultry compounded feed, the conventional poultry compounded feed is 16% by weight of crude protein as a poultry compounded commercially manufactured and sold on the market. 3% by weight crude fat. A blended feed having a content of at least 6.5% by weight of crude fiber and at least 3.25% by weight of calcium and at least 0.37% by weight of phosphorus is suitable, and any commercially available poultry feed may be used.

The blending ratio of the feed additive according to the present invention is about 0.01 to 55 parts by weight, preferably about 0.1 to 35 parts by weight, more preferably about 1 to about 25 parts by weight, based on 100 parts by weight of conventional poultry feed. do.

The feed additive and the feed composition containing the same according to the present invention includes Newcastle disease of poultry In addition to the prevention and treatment of viral diseases and at the same time show the effect of boosting the poultry immunity can be usefully used as a safe and effective feed additive and feed composition for poultry rearing.

In addition, the feed additive and the feed composition containing the same according to the present invention increases the weight of the poultry, can be provided as a high-quality poultry feed for high safety to increase the productivity or growth rate of the poultry, reducing mortality as well as Poultry's Newcastle disease, even when served as a drink of poultry It has the advantages of preventing and treating viral diseases and at the same time exhibiting the effects of immunopotentiation of poultry.

Figure 1 is a photograph showing the process of experiment

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the examples described below do not limit the present invention.

≪ Example 1 >

8 g of licorice crushed with a crusher, 8 g of wormwood, 8 g of garlic, 8 g of onions, 10 g of fresh root, 10 g of rhubarb, 10 g of rhubarb, 10 g of ginkgo, 14 g of aloe vera and 14 g of soybean seed, add 1 liter of 60 wt% ethanol solution, The mixture was extracted under reflux for 18 hours, filtered, and centrifuged at 4 ° C. 3,000 rpm for 30 minutes to remove precipitates, and the supernatant was separated.

The separated supernatant was evaporated to dryness under reduced pressure to completely remove the ethanol component, 200 ml of distilled water was added, and then freeze-dried with a freeze dryer (EYELA FDU-1100) to obtain a composite herbal extract powder (4 for sample). Refrigerated at ℃).

<Example 2>

The feed composition for poultry was prepared by uniformly mixing 99g of commercial feed for poultry in 1 g of the composite herbal extract prepared in <Example 1>.

<Test Example>

Newcastle disease virus (NDV) uses ND Virus from an outdoor farm isolated from Banseok LTC Co., Ltd. 10 days old SPF eggs were tested (see Figure 1 photo).

In order to test the in vitro virucidal activity (Virucidal activity) for the feed additive according to the present invention, the composite herbal extract obtained in Example 1 was used as a sample. 1 g of the composite herbal extract, which is the sample powder, is dissolved in 100 ml of distilled water to make a 100-fold dilution sample, and the 100-fold dilution sample is diluted 10-fold and 20-fold with distilled water, respectively, to a 1,000-fold dilution sample and a 2,000-fold dilution sample. The 100-fold diluted sample was diluted 40-fold with distilled water to obtain a 4,000-fold diluted sample.

2.5 ml of the virus solution prepared at intervals of 1 minute in a test tube containing 2.5 ml of the sample dilution solution was mixed in a test tube, and reacted for exactly 30 minutes, and shaken well at 10 minute intervals. The control group used distilled water as a sample.

25 μl of the diluted sample and the control sample were inoculated into 50 eggs for each test group. After inoculation, the cells were incubated for 3 days at 37 ° C. incubation period and examined at 24 hours, 48 hours, and 72 hours after the inoculation to observe the number and status of the stop eggs for each time unit. The results are shown in Table 1 below.

As shown in Table 1, the control group, ie, fertilized eggs inoculated with Newcastle disease virus, showed a survival rate of 68% with 16 out of 50 eggs, and decreased development of chicks, but 1,000-fold, 2,000-fold, In the fertilized eggs inoculated with 4,000-fold dilution with the ND virus at a 1: 1 ratio for 30 minutes, that is, inoculated with the sample according to the present invention, stop eggs were reduced, and embryonic development was normally observed.

In particular, the experimental group diluted 1,000-fold was able to obtain the best results with a survival rate of 98% due to the occurrence of one stop egg, and 2,000-fold, 4,000-fold survived 86% and 76%, respectively, and the survival rate decreased as the sample contained less. I could see that.

As shown in the test results, it can be confirmed that the feed additive (composite herbal extract) according to the present invention has virucidal efficacy in fertilized eggs.

Claims (9)

Licorice 4-8 wt%, Mugwort 4-8 wt%, Garlic 5-9 wt%, Onion 5-9 wt%, Produce 7-10 wt%, Brown root 7-10 wt%, Astragalus 8-10 wt%, Ginkgo Poultry with virucidal efficacy against the Newcastle disease virus of poultry, characterized in that it comprises an extract obtained from a herbal medicine consisting of 8 to 10% by weight, 11 to 15% by weight of aloe vera and 11 to 15% by weight of seeds of soybeans. Feed additives for. After drying or freezing licorice, mugwort, garlic, onion, spawn, brown root, astragalus, ginkgo, aloe vera and bean seed, respectively, immerse and crush mixed powder material in water for 10 to 50 hours, and then extract solvent Newcastle disease of poultry, characterized in that extracted with, filtered, centrifuged, concentrated and freeze-dried Method for preparing poultry feed additive having virucidal efficacy against virus. The mixed powder material is licorice 4 to 8% by weight, mugwort 4 to 8% by weight, garlic 5 to 9% by weight, onion 5 to 9% by weight, produce 7 to 10% by weight, 7 ~ 10% by weight Newcastle disease of poultry, characterized in that it is mixed with 8 to 10% by weight, 8 to 10% by weight of ginkgo, 11 to 15% by weight of aloe vera and 11 to 15% by weight of seeds of soybeans. Method for preparing poultry feed additive having virucidal efficacy against virus. The method of claim 2 or 3, wherein the extraction solvent is selected from purified water (water), a lower alcohol having 1 to 4 carbon atoms or a mixed solvent of purified water (water) and a lower alcohol having 1 to 4 carbon atoms. Newcastle disease Method for preparing poultry feed additive having virucidal efficacy against virus. The poultry Newcastle disease according to claim 2 or 3, wherein the extraction solvent is purified water (water), and hot water is extracted at 50 ° C to 150 ° C. Method for preparing poultry feed additive having virucidal efficacy against virus. Feed composition having a virucidal efficacy against poultry Newcastle disease virus, characterized in that the feed additive of any one of claims 1 to 3 in combination with a conventional feed. The feed composition of claim 6, wherein the feed additive is blended at 0.1 to 55 parts by weight based on 100 parts by weight of a conventional feed. Newcastle disease of poultry, characterized in that obtained by diluting the feed additive of claim 1 with water Poultry drinking water with virucidal efficacy against the virus. 9. The Newcastle disease of poultry according to claim 8, wherein the dilution concentration of the feed additive to water is 0.01 to 99.99% by weight. Poultry drinking water with virucidal efficacy against the virus.

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