KR20040072542A - Bactericidal composition - Google Patents

Bactericidal composition Download PDF

Info

Publication number
KR20040072542A
KR20040072542A KR1020040057411A KR20040057411A KR20040072542A KR 20040072542 A KR20040072542 A KR 20040072542A KR 1020040057411 A KR1020040057411 A KR 1020040057411A KR 20040057411 A KR20040057411 A KR 20040057411A KR 20040072542 A KR20040072542 A KR 20040072542A
Authority
KR
South Korea
Prior art keywords
fruit
grapefruit
lemon
orange
calcium chloride
Prior art date
Application number
KR1020040057411A
Other languages
Korean (ko)
Inventor
서인원
권영중
서정인
Original Assignee
넥스콘파라미터 주식회사
서인원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 넥스콘파라미터 주식회사, 서인원 filed Critical 넥스콘파라미터 주식회사
Priority to KR1020040057411A priority Critical patent/KR20040072542A/en
Publication of KR20040072542A publication Critical patent/KR20040072542A/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
    • A01N25/06—Aerosols
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
    • A61L2/0082—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using chemical substances

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Inorganic Chemistry (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Dispersion Chemistry (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

PURPOSE: A bactericidal composition is provided, thereby effectively killing bacteria on the hand and foot without generating organic compounds and stimulation of the skin. CONSTITUTION: The bactericidal composition comprises the fruit skins and seed extracts of at least one selected from grapefruit, lemon, orange, fortunella and poncirus, sodium silicate, calcium chloride and magnesium sulfate, wherein the fruit skins and seed extracts contain organic acids such as ascorbic acid, dehydro ascorbic acid and citric acid, amino acids such as glycine, alanine and glutamine, sterol compounds, saccharides such as glucose, liposoluble vitamins such as tocopherol, naringin and citral; the ratio of sodium silicate, calcium chloride and magnesium sulfate is 1:0.1-0.2:1. The method for preparing the bactericidal composition comprises the steps of: preparing 0.1 to 0.2% inorganic salt solution containing sodium silicate, calcium chloride and magnesium sulfate, and adding 2 to 20 wt.% of fruit skins and seed extracts of at least one selected from grapefruit, lemon, orange, fortunella and poncirus.

Description

살균제 조성물{omitted}Fungicide composition

본 발명은, 살균 조성물에 관한 것으로서, 보다 상세하게는 손, 발등 인체에 직접 분사될때, 대장균, 살모렐라, 및 포도상구균등의 병원성 세균에 대하여 강력한 살균력을 나타내면서, 악취, 증발 유기화합물(vapor organic compound:VOC), 및 눈과 피부에 자극을 주지 않는, 인체와 환경에 안전한 실내용 살균제 조성물에 관한 것이다.The present invention relates to a bactericidal composition, and more particularly, when sprayed directly into the human body such as hands and feet, it exhibits strong bactericidal power against pathogenic bacteria such as Escherichia coli, salmonella, and Staphylococcus aureus. compound (VOC), and an indoor fungicide composition safe for humans and the environment that does not irritate eyes and skin.

산업화, 도시화가 이루어 지면서, 사람들의 생활 환경이 매우 조밀하여지고, 실내화되고 있다. 사람들의 일생 생활을 돌아보면, 가정, 학교, 직장, 휴식, 여과, 회합, 이동, 식사등의 일상 생활의 거의가 실내에서 이루어 지고 있으며, 그 집합의 밀집도는 과거와는 비교할수 없을 정도는 고 밀도화되어 있다.As industrialization and urbanization take place, people's living environments are becoming very dense and indoor. Looking back at people's life, most of their daily lives such as home, school, work, rest, filtration, meetings, moving, and eating are done indoors. It is densified.

이러한 환경하에, 질병의 이동 속도는, 2003년의 사스, 2004년 조류 독감의 예에서 보듯이 상상을 초월할 정도로 가공적이다.Under these circumstances, the rate of movement of the disease is beyond the imagination as seen in the example of SARS in 2003 and bird flu in 2004.

이에 따라, 위생 관리는 개인의 차원에서 공중의 차원으로 이루어지지 않으면 않되게 되었다.Accordingly, hygiene management has to be done from the individual level to the public level.

본 출원인은, 이미 이에 대하여, 식당, 까페, 극장, 대합실등에 간단히 설치하여, 개인의 의복이나, 손, 발등에 직접적으로, 살균제를 분무할수 있는 시스템을 고안한바 있다(대한민국 실용신안 등록 제0334508호), 이 시스템은, 사람이 이 시스템의 일정 거리내로 접근하였을때, 인지하여, 시스템내에 내장되어 있는 살균 조성물을 에어로졸(aerosol) 형태로 분무하는 것이다.The present applicant has already devised a system that can be easily installed in restaurants, cafes, theaters, waiting rooms, etc., and sprays disinfectants directly on individual clothes, hands, and feet (Korean Utility Model Registration No. 0334508). This system, when a person approaches within a certain distance of the system, recognizes and sprays an antiseptic composition embedded in the system in the form of an aerosol.

따라서, 이 시스템에 사용할수 있는 살균 조성물은 손, 발등 인체에 직접 분사될때, 대장균, 살모렐라, 및 포도상구균등의 병원성 세균에 대하여 강력한 살균력을 나타내면서, 악취, 증발 유기화합물(vapor organic compound:VOC), 및 눈과 피부에 자극을 주지 않는, 인체와 환경에 안전하여야 한다.Therefore, the sterilizing composition which can be used in this system shows strong sterilizing ability against pathogenic bacteria such as Escherichia coli, Salmorella, and Staphylococcus when sprayed directly on the human body such as hands, feet, odor, vapor organic compound (VOC). ), And it should be safe for human body and environment without irritating eyes and skin.

현재, 이의 목적으로 개발/이용되고 있는 살균제제들은 대부분 에틸알콜, 이소프로필알콜등의 알콜 화합물, 글루타르알데히드, 과초산등의 화합물등으로 구성되어 있어, 냄새가 심하고, 피부와 안구의 자극이 심하고, 심할 경우에는 알러지를유발시키는등의 문제점이 있다.Currently, the disinfectants developed / used for this purpose are mostly composed of alcohol compounds such as ethyl alcohol and isopropyl alcohol, compounds such as glutaraldehyde and peracetic acid, and have a bad smell and irritate skin and eyes. Severe and severe cases cause problems such as allergies.

이의 문제점들을 해결하기 위하여, 천연물 유래의 물질(예를 들어, 귤과 과일의 오일, 소나무, 삼나무에서 분리한 테라핀 화합물)들이 소개되고는 있지만, 그 작용 효과도 의문시 될 뿐만 아니라 이들은 대부분 수용성이 아니라 알콜, 또는 유기 용매에 용해됨으로서, 분무시 증발 유기 화합물(VOC)이 필연적으로 발생되는 문제점이 있다.In order to solve these problems, natural-derived substances (eg, terapine compounds isolated from tangerine and fruit oils, pine and cedar) have been introduced, but the effect of these is also questioned and they are mostly water soluble. Rather than being dissolved in alcohols or organic solvents, there is a problem that evaporation organic compounds (VOC) inevitably occur during spraying.

본 발명에 있어서, 자몽, 레몬, 오렌지, 금귤, 탱자로 이루어지는 군에서 적어도 한 종류 이상 선택되어지는 과실의 과피 및 씨앗의 추출물은 아스코르빈산(ascorbic acid), 디하이드로 아스코르빈산(dehydro ascorbic acid), 구연산(citric acid)등의 유기산, 글리신, 알라닌, 글루타민산등의 아미노산, 스테롤 화합물류, 글루코스등의 당류, 토코페롤(tocoperol)등의 지용성 비타민류등 이외에 나린진(naringin) 및 씨트랄(citral) 화합물이 함유되어 있다.In the present invention, grapefruit, lemon, orange, kumquat, extract of the fruit peel and seeds of at least one kind selected from the group consisting of ascorbic acid (ascorbic acid), dehydro ascorbic acid (dehydro ascorbic acid) ), Organic acids such as citric acid, amino acids such as glycine, alanine, glutamic acid, sterol compounds, sugars such as glucose, and fat-soluble vitamins such as tocoperol, in addition to naringin and citral Compound is contained.

이들 추출물중 자몽 추출액은 항균 효과의 연구의 연구가 많이 수행되어졌다. 예를 들어, 대한민국 특허 공개공보 제2000-0061호에는 10％농도에서 대장균과 흑곰팡이에 대하여 약간의 살균력을 지니는 것이 개시 되어 있고, 최종덕등은 한국수산학회지 제 23권 제 4호(1999년 9월)에 50ppm이상의 농도에서 비브리오균등의 세균과 흑곰팡이등의 공팡이의 생육이 저해되는 것을 보고 한 바 있다.Among these extracts, grapefruit extract has been studied for its antimicrobial effect. For example, Korean Patent Laid-Open Publication No. 2000-0061 discloses that it has some bactericidal power against E. coli and black mold at a concentration of 10%. It has been reported that growth of bacteria such as Vibrio bacteria and fungi such as black mold is inhibited at a concentration of 50 ppm or more.

그러나, 상기한 자몽추출액의 10％농도에서의 살균 기능 및 50ppm이상의 농도에서의 생육 억제 기능에도 불구하고, 이들 자몽, 레몬, 오렌지, 금귤, 탱자로이루어지는 군에서 적어도 한 종류 이상 선택되어지는 과실의 과피 및 씨앗의 추출물을 본 발명의 목적에 따른 살균제로 사용하기에는 그 사용 농도가 지니치게 높은 문제점이 있다.However, despite the bactericidal function at 10% concentration of the grapefruit extract and the growth inhibiting function at a concentration of 50 ppm or more, at least one kind of fruit selected from the group consisting of grapefruit, lemon, orange, kumquat and tanza In order to use the extract of the pericarp and the seed as a fungicide according to the object of the present invention, there is a problem that the use concentration is too high.

더욱, 본 발명의 목적에 따른 살균제는 에어로졸 형태로 분무되었을때, 수분이내 즉효성의 살균력이 나타나도록 요구 받고 있다.Further, the fungicides according to the object of the present invention, when sprayed in the form of an aerosol, is required to exhibit an immediate sterilizing power within minutes.

이와 같은 본 발명의 요구 조건은, 상기한 자몽, 레몬, 오렌지, 금귤, 탱자로 이루어지는 군에서 적어도 한 종류 이상 선택되어지는 과실의 과피 및 씨앗의 추출물에 규산소다(sodium silicate), 염화칼슘(calcium chloride), 및 황산 마그네슘(magnesium sulfate)을 필수 성분으로 하여 이루어지는 무기염 혼합물을 첨가함으로서 이루어 진다.Such a requirement of the present invention, sodium silicate, calcium chloride (calcium chloride) in the extract of the fruit skin and seeds of at least one selected from the group consisting of grapefruit, lemon, orange, kumquat, tanger ) And an inorganic salt mixture consisting of magnesium sulfate as an essential component.

보다 상세하게는 규산소다(sodium silicate), 염화칼슘(calcium chloride), 및 황산 마그네슘(magnesium sulfate)의 구성비가 1: 0.1∼0.2:1으로 이루어진 무기염 혼합물을 0.1∼0.2％ 수용액으로 조제한후, 이를 상기한 자몽, 레몬, 오렌지, 금귤, 탱자으로 이루어지는 군에서 적어도 한 종류 이상 선택되어지는 과실의 과피 및 씨앗의 추출물에 대하여 2내지 20중량배 첨가하는 것이다.More specifically, an inorganic salt mixture composed of sodium silicate, calcium chloride, and magnesium sulfate in an amount of 1: 0.1 to 0.2: 1 is prepared in an 0.1 to 0.2% aqueous solution, and then The grapefruit, lemon, orange, kumquat, tanza is added to 2 to 20% by weight with respect to the extract of the skin and seeds of at least one selected from the group consisting of.

상기한 자몽, 레몬, 오렌지, 금귤, 탱자로 이루어지는 군에서 적어도 한 종류 이상 선택되어지는 과실의 과피 및 씨앗의 추출물은 글리세린 추출물으로서, 추출물의 함량이 최소한 40중량％인 액상물질으로, 물 또는 알콜에 쉽게 용해 분산되어 진다.Extracts of fruit and seeds of the fruit selected from at least one kind from the group consisting of grapefruit, lemon, orange, kumquat, tanger are glycerin extract, liquid content of at least 40% by weight of the extract, water or alcohol Easily dissolved and dispersed in

도1은 본 발명에 의해 조성된 살균제 조성물을분무 처리된 손에 대한 대장균에 검출 비교사진이다.Figure 1 is a comparative photograph of the detection of E. coli in the spray-treated hands of the fungicide composition prepared by the present invention.

도2는 본 발명에 의해 제조된 살균 조성물의 대장균에 대한 처리 전후의 고체 배지에서의 배양 실험 결과 비교사진이다.Figure 2 is a comparison of the results of the culture experiment in a solid medium before and after treatment for E. coli of the bactericidal composition prepared according to the present invention.

도3은 본 발명에 의해 제조된 살균 조성물의 살모렐라 균주에 대한 처리 전후의 고체 배지에서의 배양 실험 결과 비교사진이다.Figure 3 is a comparison of the results of the culture experiment in a solid medium before and after the treatment for the Salmorella strain of the bactericidal composition prepared by the present invention.

도4는 본 발명에 의해 조성된 살균제 조성물의 포도상구균 균주에 대한 처리 전후의 고체 배지에서의 배양 실험 결과 비교사진이다Figure 4 is a comparison of the results of the culture experiment in a solid medium before and after treatment with the Staphylococcus strain of the fungicide composition prepared by the present invention

상기한 본발명의 조성물은 상기한 자몽, 레몬, 오렌지, 금귤, 탱자로 이루어지는 군에서 적어도 한 종류 이상 선택되어지는 과실의 과피 및 씨앗의 추출물 0.05∼0.5v/v％ 수용액에 규산소다(sodium silicate), 염화칼슘(calcium chloride), 및 황산 마그네슘(magnesium sulfate)의 구성비가 1: 0.1∼0.2:1으로 이루어진 무기염 혼합물을 0.1∼0.2w/v％ 첨가하였을 때, 에어로졸로 분사되어, 접촉면에 1내지 5분이내에 90％이상의 살균 효과를 나타낸다.The composition of the present invention is sodium silicate in an aqueous solution of 0.05-0.5v / v% of fruit and seeds of fruit selected from at least one selected from the group consisting of grapefruit, lemon, orange, kumquat, tanger. ), Calcium chloride, and magnesium sulfate, the composition ratio of 1: 0.1 to 0.2: 1 when the inorganic salt mixture of 0.1 to 0.2 w / v% is added, it is sprayed with an aerosol, 1 to the contact surface It exhibits a bactericidal effect of 90% or more within 5 minutes.

이하, 본 발명을 보다 쉽게 이해하기 위하여, 본 발명의 바람직한 실시예를 제시한다. 그러나, 이 실시예는 본 발명을 보다 쉽게 이해하기 위하여 제시되는 것일뿐, 본 발명이 이 실시예에 한정되는 것은 아니다.Hereinafter, in order to more easily understand the present invention, preferred embodiments of the present invention are presented. However, this embodiment is only presented to more easily understand the present invention, the present invention is not limited to this embodiment.

(실시예)(Example)

추출물의 함량이 40％인 자몽씨 글리세린 추출물을 시중에서 구입하여(상품명:DF100) 0.05％의 수용액을 제조 하였다. 이 자몽 추출물의 수용액에 규산소다 0.175％, 염화 칼슘 0.025％, 황산 마그네슘 0.175％로 이루어진 무기염 수용액을 1％되도록 첨가하여 본 발명에 따른 살균제 조성물을 제조 하였다.A grapefruit seed glycerin extract having a content of 40% of the extract was commercially available (trade name: DF100) to prepare an aqueous solution of 0.05%. To the aqueous solution of the grapefruit extract was added an aqueous inorganic salt solution consisting of 0.175% sodium silicate, 0.025% calcium chloride and 0.175% magnesium sulfate to 1% to prepare a fungicide composition according to the present invention.

이 살균 조성물(294g)을 스틸캔(500㎖)내에 넣고, 액화 석유가스(70g)로 충전하여, 에어로졸 분사체를 제조하여, 아래와 같은 평가 시험을 행하였고, 그 결과를 다음에 나타낸다.This sterilizing composition (294 g) was placed in a steel can (500 ml), filled with liquefied petroleum gas (70 g) to prepare an aerosol spray body, and the following evaluation test was carried out. The results are shown below.

1)살균 시험1) sterilization test

상기 실시예에서 제조된 에어로졸 분사체로 사람의 손에 직접 2내지 3초간 분무한후, 대한민국 (주)이지바이오시스템사에서 제조한 대장균 현장 검사 시스템핸드플레이트(상품명:Easy Media)에 검체(손)를 직접 접촉시킨후, 제조사의 메뉴엘에 따라 24시간 배양한후, 대조군과 비교하였다. 이 결과를 사진을 찍어 도1에 나타내었다.After spraying the human hand directly with the aerosol injector prepared in the above example for 2-3 seconds, E. coli on-site inspection system hand plate (trade name: Easy Media) manufactured by Easy Bio Systems Co., Ltd. After direct contact, incubated for 24 hours according to the manufacturer's menuel, and compared with the control. This result was taken and shown in FIG.

도1의 (나)에서 보듯이 본 발명에 따라 제조된 살균 조성물로 분부된 손의 경우 균의 군집이 발견되지 않은 반면, 분무되지 않은 대조군(가)에 있어서는 수많은 군의 군집이 발견되었다.As shown in (b) of FIG. 1, in the case of hands distributed with the sterilizing composition prepared according to the present invention, no community of bacteria was found, whereas in the non-sprayed control group (a), a large number of populations were found.

또한, 본 발명에 따른 살균 조성물을 대장균균주(ATCC25922), 살모렐라균주(ATCC27562), 포도상구균주(ATCC6538)가 각각 1×108cfu/㎖ 되도록 접종한 고체 배지상에 각각 1분, 5분, 10분간 접촉시키었을때, 모든 경우 공히 99.6％이상의 살균 효과를 나타내었다.In addition, the bactericidal composition according to the present invention was inoculated on a solid medium inoculated with Escherichia coli strain (ATCC25922), Salmonella strain (ATCC27562), and Staphylococcus strain (ATCC6538) to 1 × 10 8 cfu / ml, respectively, for 1 minute and 5 minutes, respectively. When contacted for 10 minutes, all cases showed more than 99.6% sterilization effect.

이 결과는 도2내지 도4의 결과로 또한 나타낸다. 도2내지 도4에서 (가)는 처리전, (나)는 처리후의 사진으로 99％이상의 살균균되었음을 보여주는 것이다.This result is also shown as the result of FIGS. In Figures 2 to 4 (a) before the treatment, (b) shows that more than 99% bactericidal bacteria in the photograph after the treatment.

2)안전성 시험2) safety test

상기 실시예에서 제조된 살균 조성물은 대한민국 서울시 감남구 신사동에 소재한 한국기기유화시험연구원에 제공하고, 국소자극시험(피부자극), 국소자극시험(안점막), 급성 경구시험을 식품의약품안전청 고시 제1999-61호(1999.1.22)의 시험 방법으로 평가한 결과, 피부자극도(P.I.I 0.125) 비자극성, 안점막 자극도(A.O.I 1.00) 무자극물, LD502,000mg/kg(ICR계 마우수 암수 10마리씩, 투여후 14일간 모두 생존) 의 매우 안전한 물질임을 평가 받았다.The bactericidal composition prepared in the above example was provided to the Korea Institute of Emulsification Testing, located in Sinsa-dong, Gamnam-gu, Seoul, Korea. Evaluation by the test method of 1999-61 (1999.1.22), skin irritation degree (PII 0.125) non-irritant, eye irritation degree (AOI 1.00) non-irritant, LD 50 2,000mg / kg (ICR-based Mausu male and female 10 animals, all 14 days post-dose) were evaluated as a very safe substance.

상술한바와 같이 본 발명에 의하면 인체의 손, 발등에 직접 분사할 때, 대장균, 살모렐라 및 포도상 구균등의 병원성 세균에 대하여 강력한 살균력을 나타내면서 악취, 증발 유기화합물이 발생되지 않으며, 눈과 피부에 자극을 주지 않는등 신규하고 유효한 발명인 것이다.As described above, according to the present invention, when sprayed directly on the human hand or foot, it exhibits a strong bactericidal power against pathogenic bacteria such as Escherichia coli, salmonella and staphylococcus, and does not generate odors or evaporated organic compounds. It is a novel and effective invention without giving any stimulus.

Claims (7)

자몽, 레몬, 오렌지, 금귤,탱자로 이루어지는 군에서 적어도 한 종류 이상 선택되어지는 과실의 과피 및 씨앗의 추출물과 규산소다(sodium silicate), 염화칼슘(calcium chloride), 및 황산 마그네슘(magnesium sulfate)을 필수 성분으로 하여 이루어지는 무기염 혼합물로 구성되는 것을 특징으로하는 살균제 조성물.At least one fruit and seed extract, sodium silicate, calcium chloride, and magnesium sulfate selected from the group consisting of grapefruit, lemon, orange, kumquat and tan are essential A fungicide composition comprising an inorganic salt mixture comprising the ingredient. 제 1항에 있어서,The method of claim 1, 자몽, 레몬, 오렌지, 금귤, 탱자로 이루어지는 군에서 적어도 한 종류 이상 선택되어지는 과실의 과피 및 씨앗의 추출물은 아스코르빈산(ascorbic acid), 디하이드로 아스코르빈산(dehydro ascorbic acid), 구연산(citric acid)등의 유기산, 글리신, 알라닌, 글루타민산등의 아미노산, 스테롤 화합물류, 글루코스 등의 당류, 토코페롤(tocoperol)등의 지용성 비타민류등 이외에 나린진(naringin) 및 씨트랄(citral) 화합물을 함유함을 특징으로 하는 살균제 조성물.Extracts of fruit and seeds of at least one fruit selected from the group consisting of grapefruit, lemon, orange, kumquat and tan are ascorbic acid, dehydro ascorbic acid and citric acid organic acids such as glycine, alanine, glutamic acid, sterol compounds, sugars such as glucose, fat-soluble vitamins such as tocoperol, and others, including naringin and citral compounds. Disinfectant composition characterized by. 제 1항에 있어서,The method of claim 1, 무기염 혼합물은 규산소다(sodium silicate), 염화칼슘(calcium chloride), 및 황산 마그네슘(magnesium sulfate)의 구성비가 1: 0.1∼0.2:1으로 이루어짐을 특징으로 하는 살균제 조성물.The inorganic salt mixture is a disinfectant composition, characterized in that the composition ratio of sodium silicate, calcium chloride, and magnesium sulfate is 1: 0.1 to 0.2: 1. 제 1항에 있어서,The method of claim 1, 무기염 혼합물을 0.1∼0.2％ 수용액으로 조제한후, 이를 상기한 자몽, 레몬, 오렌지, 금귤, 탱자로 이루어지는 군에서 적어도 한 종류 이상 선택되어지는 과실의 과피 및 씨앗의 추출물에 대하여 2내지 20중량배 첨가하여 이루어짐을 특징으로 하는 살균제 조성물.After preparing the inorganic salt mixture in a 0.1 to 0.2% aqueous solution, it is 2 to 20% by weight with respect to the extract of the fruit peel and seeds of at least one selected from the group consisting of grapefruit, lemon, orange, kumquat, tanger Disinfectant composition, characterized in that the addition is made. 제 1항에 있어서,The method of claim 1, 자몽, 레몬, 오렌지, 금귤, 탱자로 이루어지는 군에서 적어도 한 종류 이상 선택되어지는 과실의 과피 및 씨앗의 추출물은 글리세린 추출물으로서, 추출물의 함량이 최소한 40중량％인 액상물질으로, 물 또는 알콜에 쉽게 용해 분산되어 지는 것임을 특징으로 하는 살균제 조성물.Extracts of fruit and seeds of fruit selected from at least one selected from the group consisting of grapefruit, lemon, orange, kumquat and tangerine are glycerin extracts, which are liquid substances with an extract content of at least 40% by weight, and are easily soluble in water or alcohol. Disinfectant composition, characterized in that it is dissolved and dispersed. 제 1항에 있어서,The method of claim 1, 살균제 조성물은 자몽, 레몬, 오렌지, 금귤, 탱자로 이루어지는 군에서 적어도 한 종류 이상 선택되어지는 과실의 과피 및 씨앗의 추출물 0.05∼0.5v/v％ 수용액에 규산소다(sodium silicate), 염화칼슘(calcium chloride), 및 황산 마그네슘(magnesium sulfate)의 구성비가 1: 0.1∼0.2:1으로 이루어진 무기 혼합물을 0.1∼0.2w/v％ 첨가하여 이루어 짐을 특징으로 하는 살균제 조성물.The fungicide composition comprises sodium silicate and calcium chloride in an aqueous solution of 0.05-0.5v / v% of fruit and seeds of fruit selected from the group consisting of grapefruit, lemon, orange, kumquat and tangerine. ), And 0.1 to 0.2 w / v% of an inorganic mixture composed of 1: 0.1 to 0.2: 1 of magnesium sulfate. 제1항 또는 제 6항중 어느 하나의 항에 있어서,The method according to any one of claims 1 to 6, 상기한 살균 조성물은, 추출물의 함량이 40％인 자몽씨 글리세린 추출물 0.05％의 수용액에 규산소다 0.175％, 염화 칼슘 0.025％, 황산 마그네슘 0.175％로 이루어진 무기염 수용액을 1％되도록 첨가하여, 이루어 지는 것임을 특징으로 하는 살균제 조성물.The above sterilizing composition is prepared by adding an aqueous inorganic salt solution consisting of 0.175% of sodium silicate, 0.025% of calcium chloride, and 0.175% of magnesium sulfate to 1% of an aqueous solution of 0.05% of grapefruit seed glycerin extract having a content of 40%. Fungicide composition, characterized in that.
KR1020040057411A 2004-07-22 2004-07-22 Bactericidal composition KR20040072542A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020040057411A KR20040072542A (en) 2004-07-22 2004-07-22 Bactericidal composition

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020040057411A KR20040072542A (en) 2004-07-22 2004-07-22 Bactericidal composition

Publications (1)

Publication Number Publication Date
KR20040072542A true KR20040072542A (en) 2004-08-18

Family

ID=37360057

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020040057411A KR20040072542A (en) 2004-07-22 2004-07-22 Bactericidal composition

Country Status (1)

Country Link
KR (1) KR20040072542A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101889273B1 (en) * 2017-03-24 2018-08-20 김오승 Antimicrobial composition and funeral products using thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101889273B1 (en) * 2017-03-24 2018-08-20 김오승 Antimicrobial composition and funeral products using thereof

Similar Documents

Publication Publication Date Title
CN102657245B (en) Disinfectant prepared from plant extracts as well as preparation method for disinfectant
CA2549307C (en) Essential oils based disinfecting compositions having tuberculocidal and fungicidal efficacies
WO2006087569A2 (en) Antimicrobial composition comprising an extract of tarchonanthus camphoratus
SG173940A1 (en) Air sanitizer and malodour removal formulation
CN106619213A (en) Preparation method of controlled-release natural plant mosquito repellent
CN104173248B (en) Natural plant essential oil-containing disinfecting liquid soap, as well as preparation method and application thereof
US20060075922A1 (en) Controlled-acidity composition
CN105028452A (en) Air disinfecting spray solution and preparation method of same
CN101766186A (en) Composition capable of slowly-releasing chlorine dioxide and application thereof
JP2004509636A (en) Water-based disinfectant / disinfectant for food
US8445030B2 (en) Persistent and fast acting antiseptics and disinfectants based on calcium fluoride
RU2407547C2 (en) Agent for disinfecting and sanitising air
RU2647836C1 (en) Composition for disinfection and aromatization of air
KR20080081633A (en) Antimicrobial soap composition
JP5241905B2 (en) Deodorant formulated with an efficacy enhancer for organic deodorant ingredients
RU2363158C1 (en) Biocidal composition for soaking napkins
KR20040072542A (en) Bactericidal composition
CN110946159A (en) Plant essence biological compound clothing mildew sweat odor removing agent
JP4896538B2 (en) Potency enhancer for organic deodorant ingredients.
CN103283788A (en) High-efficiency environment-friendly mosquito repelling dew
JPH10114603A (en) Sustainable alcoholic germicide
CN106420410A (en) Preparing method of fresh and scent type natural botanical mosquito repellant liquor
CN113521042A (en) Alcohol-free wash-free virus inactivation disinfectant special for children and preparation method thereof
KR100512871B1 (en) Agent for expelling noxious insects and worms and toilet paper produced using the agent
JPH07252105A (en) Liquid disinfectant

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E601 Decision to refuse application