KR20040062273A - Composition comprising the extracts of Longanae arillus, Zizyphi spinosae semen, Polygalae radix and Poria cocos for the enhancement of memory and the prevention and treatment of dementia - Google Patents

Abstract

PURPOSE: Provided is a composition for increasing memory and preventing and treating dementia, which composition essentially contains the mixed extract of Euphoria longana, Zizyphus jujuba Miller, Polygalae Radix and Poria cocos. CONSTITUTION: A composition for increasing memory and preventing and treating dementia, essentially contains Euphoria longana, Zizyphus jujuba Miller, Polygalae Radix and Poria cocos. It further contains at least one selected from the group consisting of Angelica gigas Nakai, ginseng radix, Astragalus membranaceus (Fisch.) Bunge, Atractylodes japonica(Koidz) Kitag, Inula Helenium L., licorice roots, ginger and Jujubae Fructus.

Description

Composition comprising the extracts of Longanae arillus, Zizyphi spinosae semen, Polygalae radix and Poria cocos for the enhancement of memory and the prevention and treatment of dementia}

The present invention relates to a herbal composition for memory enhancement containing the extract as an active ingredient, specifically, extracts of longan meat, Sanjoin, Wonji and baeksinsin as an active ingredient to enhance memory, brain cell proliferation effect involved in memory The present invention relates to a composition for preventing and treating excellent learning ability and memory improvement, forgetfulness or dementia.

Memory refers to storing and recalling the results of experiences or information obtained from the environment (William F Ganong; Ganong's Physiology, Seoul, Hanwoori, p289, pp291-292, 1999). The process of memory is divided into three stages: encoding, reinforcement, and withdrawal. In this process, not only the exchange of chemical substances at the cerebral endoscopy, but also the structural change of the brain occurs in the process of converting short-term memory into long-term memory. Known (Belcheva I et al .; Peptides , 21 (3) , pp407-411, 2000).

Learning in psychology is a specific change in the organism that has occurred through practice or experience, and memory refers to the ability to store information needed for mental activity and bring it out into the conscious world as needed. Because it is called memory, learning and memory are inseparable from each other, and learning can be measured only through the quantity and quality of memory (Jang Dong-hwan et al., Psychology Introduction, Seoul, Park Youngsa, p214, p267, p272, p313).

On the other hand, as the social environment is diversified, people are exposed to more stimuli and need more rapid brain activity to accommodate these stimuli. In particular, with the recent economic growth, environmental damage and water pollution caused by industrialization, industrialization, and flooding of instant foods are threatening our health condition. Absolute lack of exercise due to the development of civilization not only promotes obesity, but also causes such obesity, chronic degenerative adult diseases such as arteriosclerosis, hypertension, coronary heart disease, myocardial infarction, angina pectoris, stroke. In addition, the number of Alzheimer-type dementia patients in the elderly is increasing year by year. In addition, adolescents, especially examinees, have long-term learning activities that not only feel the brain and mind and body fatigue, but are also seriously affected by the healthy development of the brain. Therefore, as the society becomes more complex and competition for admission increases, there is an urgent need for the development of a method to clear the tired brain and improve memory and promote fatigue recovery. In recent years, the overburdened learning burden of students has emerged as a social problem.

In oriental medicine, various treatment prescriptions are recorded in various documents, including consent, and the various treatments using the prescriptions are known to help improve memory.

However, experiments with animal models on the effects of these regimens on the treatment of memory or improvement of memory have recently been published (Park Chan-Won et al; Korean Journal of Oriental Medicine, 20 (4), pp573-583, 2000).

On the other hand, Longanae Arillus used in the present invention is dried dried asters of Euphoria longan (LOUR.) STEUD. Of the fig tree (Sapindaceae), has a calming, dry, nourishing, and insomnia treatment .

Sanzoin (Zizyphi Spinosae Semen) is a seed of Zizyphus jujuba MILL., Which contains a large amount of fat, protein and two sterols. It has sedation and analgesic action, and is used as a sanitary, soothing and hypnotic in herbal medicine.

Polygalae Radix is the root of Polygala tenuifolia WILLD., A perennial herb belonging to the Polygalaceae family.

Pacococcus (Poria Cocos) is a medicinal herb that only grows through the roots of the fungus parasitic on the roots of pine. It is mainly used in oriental medicine to stabilize the mind and clear the head, and also has a diuretic effect.

Korean Patent Laid-Open Publication No. 2002-73237 discloses a composition for protecting brain cells and enhancing memory containing sessin extract, and Korean Patent Laid-Open Publication No. 2002-38381 discloses a learning and memory enhancing herbal composition comprising a wolfberry extract. have. In addition, the Republic of Korea Patent Publication No. 2002-74285 discloses a memory-enhancing herbal composition containing Sukji sulfur extract as an active ingredient.

However, it is not disclosed in the above document that the herbal medicine used in the present invention has a memory enhancing or proliferating effect of cranial nerve cells.

The present inventors have pharmacologically observed the effect of improving the memory and brain cell proliferation involved in memory of the complex herbal containing the above herbal medicines, focusing on whether there is a possibility to treat dementia for herbal medicines, which have accumulated a large amount of clinical data. Was studied.

The present inventors have developed a new herbal composition that can contribute to the improvement of memory and learning ability, and completed the present invention by confirming its effect through behavioral experiment using animal model and observation of cell proliferation in brain tissue. .

An object of the present invention is to improve the memory and learning ability, to improve memory disorders such as forgetfulness, and to provide a composition containing a herbal extract as an active ingredient effective in the prevention and treatment of dementia.

1 is a diagram observing the potential maintenance of passive avoidance experiments in rats administered the composition of the present invention,

Figure 2 is a diagram illustrating the BrdU-immune response in the sagittal brain of the rat administered the composition of the present invention.

In order to achieve the above object, the present invention yonganyuk having a brain cell proliferation effect Longanae Arillus), sanjoin (Z izyphi Spinosae Semen), sheet (Polygalae Radix) and baekboksin (crude extract of the composite herbal essentially comprises a Poria Cocos) Provides a composition for enhancing memory, improving learning ability, and preventing and treating dementia.

In addition, the present invention, in addition to the essential complexes, yongan meat, sanjoin, base and baeksinsin (Angelicae Gigantis Radix), Ginseng (Ginseng Radix), Astragali Radix, Atractylodis Macrocephalae Rhizoma, Auklandiae Radix ), Licorice (Glycyrrhizae Radix), ginger (Zingiberis Rhizoma Recens) and control (Jujubae Fructus) and the like.

A preferred composition of the present invention provides a composition containing an extract of a combination herbal comprising longan meat, Sanjoin, Wonji, Baeksin, Angelica, Ginseng, Astragalus, Baekchul, Mokji, Licorice, Ginger and Control.

The extract refers to an extract available in a polar solvent such as water, methanol, ethanol and the like and a mixed solvent thereof.

In addition, the present invention provides a pharmaceutical composition for the prevention and treatment of dementia containing the complex herbal extract as an active ingredient.

The composition for improving memory, improving learning ability, and preventing and treating dementia of the present invention comprises 0.1 to 90% by weight of the complex herbal extract of the present invention based on the total weight of the composition.

The method of separating the extract from the herbal medicine of the present invention is as follows.

Hereinafter, the present invention will be described in detail.

The herbal extract of the present invention is about 5 to 20 times, preferably about 10 to 15 times water, lower alcohol or about 1: 0.1 to 1:10, preferably about 1 kg of dried herbal weight (kg). These mixed solvents having a mixing ratio (kg / L) of 1: 0.2 to 1: 5 are used at an extraction temperature of 20 to 120 ° C, preferably 50 to 100 ° C for about 1 hour to 2 days, preferably about 2 hours to After the extraction by extraction method such as hot water extraction, ultrasonic extraction, reflux cooling extraction, etc. in about 1 day, filtered and concentrated under reduced pressure, and additionally lyophilized, according to the water, lower alcohol or mixed solvent of the present invention. A crude herbal crude extract that is a soluble extract can be obtained.

Contents of the main herbal medicines of the complex herbal medicine of the present invention, yongan meat, baekboksin, sanjoin, and raw paper are contained in 5 to 25% by weight, respectively, within the range of not more than 100% of the total weight, yongan meat: baekxin: sanjoin: original paper The composition ratio is 1-3: 1-2: 1-2: 1, preferably 1: 1: 1: 1 weight ratio (g). When these active ingredients are mixed at this composition ratio, the memory improvement, forgetfulness or dementia of the present invention can be maximized.

In the present invention, the essential yongan meat, baekboksin, sanjoin, supplements in addition to the base, donkey, ginseng, Astragalus, Baekchul, Mokyang, licorice, ginger, the control content of the total weight does not exceed 100% each in the range of 1 to 1 20% by weight, preferably 8 to 12% by weight, 8 to 12% by weight, ginseng 8 to 12% by weight, 8 to 12% by weight, 8 to 12% by weight, 4 to 8% by weight, licorice 2 to 6 weight %, Ginger 4-8% by weight, control 4-8% by weight.

In a preferred embodiment of the present invention, it provides a complex herbal extract containing longan meat, Sanjoin, Wonji, Baeksin, Angelica, Ginseng, Astragalus, Baekchul, Mokyang, Licorice, Ginger, and control, more preferably the total weight relative to the total weight Is within the range of not more than 100% of the long-term meat 5-25% by weight, 5-25% by weight of acid, 5-25% by weight of raw paper, 5-25% by weight of Baeksin, 8-12% by weight of Angelica ginseng, 8-12% by weight , 8-12% by weight, 8-12% by weight, 4-8% by weight of Mokcho, 2-6% by weight licorice, 4-8% by weight of ginger, 4-8% by weight of mixed extract do.

As a result of behavioral experiments using the passive avoidance test by administering the herbal extract of the present invention to the white rats, the potential retention time was delayed in the composite herbal group compared to the control group, indicating that memory was significantly enhanced. As a result of examining the number of proliferated neurons in the hippocampus region involved in memory of the brain tissue of the rat, it was confirmed that significantly increased in the multi-drug extract administration group of the present invention compared to the control group.

The present invention provides a pharmaceutical composition for the prevention and treatment of forgetfulness or dementia comprising the complex herbal extracts obtained in the extraction process.

Additionally, the pharmaceutical composition for memory enhancement and dementia prevention and treatment containing the complex herbal extract of the present invention comprises 0.1 to 90% by weight of the complex herbal extract based on the total weight of the composition.

The composition comprising the multi-drug extract of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

Carriers, excipients and diluents that may be included in the composition comprising the complex herbal extract of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin , Calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

The composition comprising the complex herbal extract according to the present invention is in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, external preparations, suppositories, and sterile injectable solutions, respectively, according to a conventional method. Can be formulated and used.

The amount of the combined herbal extract of the present invention may vary depending on the age, sex, and weight of the patient, but may be administered once to several times in an amount of 0.1 to 500 mg / kg. In addition, the dosage of the extract can be increased or decreased depending on the route of administration, the degree of disease, sex, weight, age and the like. Therefore, the above dosage does not limit the scope of the present invention in any aspect.

In addition, the extract of the present invention can be used as a main, secondary ingredient and food additive of other foods.

In another aspect, the present invention provides a dietary supplement for memory enhancement, learning ability improvement and dementia prevention, including extracts and food acceptable food supplement additives.

The composition containing the extract of the complex herbal including the long-term meat, sanjoin, base paper and vaccine of the present invention can be used in a variety of drugs, foods and beverages for memory enhancement and prevention and treatment of dementia. Examples of the food to which the present extract can be added include various foods, beverages, gums, teas, vitamin complexes, and health functional foods.

Extract itself of the present invention is a drug that can be used with confidence even for long-term administration for the purpose of prevention because there is little toxicity and side effects.

The complex herbal extract of the present invention may be added to food or beverage for the purpose of memory enhancement and prevention of dementia.

The health functional beverage composition of the present invention has no special limitation except for containing the extract as an essential ingredient, and may contain various flavors or natural carbohydrates, etc. as additional ingredients, as in general beverages. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of said natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and salts thereof. , Organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. The compositions of the present invention may also contain pulp for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected from the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, the present invention will be described in detail.

However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

Example 1. Manufacture of complex herbal extracts

The medicine used in the present invention was purchased from Wonkwang Pharmaceutical Co., Ltd. (Seoul, Korea), 35-40 g of Longanae arillus, 35-40 g of Zizyphi spinosae Semen, 35-40 g of Polygalae Radix, and Poria Cocos ) 35-40 g, Angelicae gigantis Radix 35-40 g, Ginseng Radix 35-40 g, Astragali Radix 35-40 g, Atractylodis macrocephalae Rhizoma 35-40 g, Aucklandiae Radix 35-40 g 40 g, licorice (Glycyrrhizae Radix) 10 to 15 g, ginger (Zingiberis Rhizoma Recens) 23 to 27 g and control (Jujubae Fructus) 1 to 3 g are added to a 10 liter round flask, 5,000 ml of distilled water is added and the chiller is connected for 2 hours. It was plunged. The whole solution was filtered on a filter paper, concentrated with a reduced pressure concentrator (EYELA Co, Japan), and lyophilized with a freeze dryer (EYELA Co, Japan) to obtain a freeze-dried product (yield: 22%). The above procedure was repeated to prepare the necessary samples during the entire experiment.

Example 2 Preparation of Experimental Animals

The animal used in the present invention is a 6-week-old male rat of the Wistar system supplied from a central laboratory animal and the weight at the time of supply was 180 to 200 g. Water and feed (Purina Inc., Seoul) was free to eat, and experimented after 10 days in a nursery environment with a temperature of 18 to 24 ℃, humidity 40 to 60%. Day and night cycles were 12 hours each and night time was from 7 am to 7 pm. The lab environment was the same as the kennel and the same environment was maintained during the experiment.

Thus, the experimental animals adapted to the laboratory environment for 10 days were divided into 10 control animals and 10 experimental animals.

While supplying enough water and feed, the control group was drinking water, and the experimental group dissolved the extract of Example 1 in drinking water at a concentration of 2g / 10ml, and administered 14 times once a day for 2 weeks (2.0g / kg / day, po ). During the behavioral test period, 16 times for a total of 16 days were administered before the retention test, that is, the same day as the first and second day of study.

Example 3. Observation of memory change by administration of extract

As a behavioral experiment to observe the memory change according to the administration of the extract of the present invention, a passive avoidance test using a shuttle box (shuttle box) was performed, the method is Da Cunha ( Braz. J. Med. Biol. Res ., 23 , pp301-306, 1990) and proceeded for 3 days in the following manner.

One hour before the start of each experiment, the animals were transferred to the behavioral laboratory and stabilized.

(3-1) Training trial day 1

The light on the left side of the passive avoidance box was turned on and the animals were placed in the left side for 10 seconds with the door between the left and right sides closed. When the door between the left and right sides is opened, the experimental animal instinctively moves to the dark right room, while closing the door between the left and right sides and searching for the right room for 1 minute, the experimental animal is taken out and moved to the cage. . The above method was repeated so that the time from the left room to the right room was less than 20 seconds.

(3-2) Acquisition trial

The left side of the manual evacuation box was turned on and the test animals were placed in the left side and closed for 10 seconds with the doors between the left and right sides closed. When the door between the left and right sides is opened, the test animal moves to the dark right room. The door between the left and right sides is closed and 2.0 mA electric stimulation is applied to the sole of the test animal for 2 seconds. When the door between the left and right sides is opened after the electrical stimulation, the test animal is avoided to the left room, where it stayed for 30 seconds and then moved to the rat breeding ground.

(3-3) Day 3 of behavioral test (retention test)

After 24 hours of electrical stimulation, the left room light of the passive avoidance box was turned on and the animals were placed in the left room for 30 seconds with the doors between the left and right sides closed. The door between the left and right sides was opened and the time taken for the test animal to enter the right room was measured. All four feet of the test animals were measured to enter the right chamber, and if not entered after 300 seconds, the retention time was recorded as 300 seconds.

In order to determine the effect of the extract of the present invention on the learning and memory ability of the rats, the results of measuring memory capacity after 24 hours of study are shown in Table 1.

As a result of the measurement, the potential latency, which is the time to hesitate to go to the right room by remembering the fear of electric stimulation in the right room, was compared with the control average of 48.70 ± 24.86 (mean ± standard deviation, n = 10) seconds. Was significantly delayed to 191.40 ± 34.58 (mean ± standard deviation, n = 10) seconds. (P = 0.003, Mann-Whitney U-test)

Potential maintenance of passive avoidance Rat number Control Extract administration group Potential hold (seconds) One 8 148 2 14 300 3 20 300 4 262 300 5 31 300 6 90 20 7 17 181 8 17 24 9 14 180 10 14 161 Mean ± Standard Deviation (sec) 48.70 ± 24.86 191.40 ± 34.58 ^** * Significant deviation from control (*: p <0.05 and **: p <0.01)

Example 4. Observation of changes in brain tissue cells following administration of extract

In order to investigate the effect of the extract of the present invention on cell proliferation of hippocampus, the memory-associated brain tissue of rats, dentate of hippocampus was obtained using immunohistochemical method using BrdU. The number of cells proliferated at the granule layer in gyrus was measured. See Park et al. , Neuroscience Letters, 309 , pp 9-12, 2001.

(4-1) BrdU injection

Based on the results of the retention test, the control group excludes the two animals with the fastest retention latency and the one with the latest one. In the experimental group, the animals with the earliest retention latency are the same. Two of the most recent animals were excluded and 8 animals of each group were selected and administered BrdU (Sigma Chemical ㏇, USA). The solution of BrdU dissolved in saline was administered intraperitoneally (50 mg / kg, i.p) 2 hours before the perfusion of the animals in the evening and the morning of the day after the retention test.

(4-2) Tissue preparation

Experimental animals were anesthetized with pentobarbital sodium (50 mg / kg, i.p) and perfused with 4% paraformaldehyde (dissolved in 0.1 M phosphate buffer). The brain was extracted and postfixed overnight with 4% paraformaldehyde. Transfer was made with 30% sucrose (dissolved in 0.05M PBS) for 5 days. Brain tissue was coronally sectioned (40 μm thick) and stored in a preservation solution of 30% ethylene glycol, 30% glycerin, and 0.05 M phosphate buffer.

(4-3) Immunocytochemical detection

Among the tissue sections in storage, three to four tissue sections of Bregma -2.56 mm to 4.16 mm per animal were selected and examined by immunohistochemical method. Immunohistochemical detection of BrdU-labeled nuclei requires DNA denaturation. Before reacting with anti-BrdU primary antibody, free-floating brain sections were pretreated with 50% formamide-2 × SSC at 65 ° C. for 2 hours and then 37 ° C. Treated in 2N HCl for 30 min. The tissues were then washed in 0.1M boric acid (pH 8.5) at 25 ° C. for 10 minutes. Washed three times each 5 minutes with 0.05M PBS. The tissue was reacted with primary antibody (primary antibody, 1: 1000 dilution, Roche Diagnostics, Mannheim, Germany) overnight at 25 ° C. The primary antibody was diluted with phosphate buffer containing 0.3% Triton X-100, 0.5 mg / mL bovine serum albumin and 1.5% normal goat serum. The tissue was reacted with a horse anti-mouse secondary antibody (1: 100 dilution, Vector, Burlingame, Calif., USA) for 90 minutes at room temperature, followed by avidin-biotin-peroxidase at room temperature for 1 hour. It was reacted with a complex (avidin-biotin-peroxidase complex, 1: 100 dilution; Vector, Burlingame, CA, USA). The tissues were reacted with 0.02% 3,3'-diaminobenzidine tetrahydrochloride, 0.01% hydrogen peroxide, 0.04% nickel chloride (NiCl ₂ ) for 3 minutes. Tissues were washed with PBS three times for five minutes each time for each reaction. Tissues were mounted on gelatin coated slides (mounted), dried sufficiently, counterstained with cresyl violet, and then mounted with polymount. After the slide was dried, the position of the dentate gyrus of the hippocampus was found at 100 times magnification by optical microscope, and the number of BrdU stained cells was counted at the granule cell layer at 400 times magnification. Tissues damaged during staining were excluded from the calculation.

(4-5) Statistical Processing

The results of the behavioral experiments were expressed as mean ± standard deviation, and the significance was assessed by the Mann-Whitney U-test using the SPSS program (SPSS 11.0 for Window, SPSS, Inc.).

As a result, as shown in Table 2, the number of proliferated cells was 21.04 ± 1.70 (mean ± standard deviation, n) compared to the control average of 10.24 ± 1.11 (mean ± standard deviation, n = 25). = 25), significantly increased. (P = 0.000, student t-test)

Effect of Extract Administration on Rat Dentate Gyrus in Rats Brain tissue section number Control Extract administration group BrdU-Immune Response Cell Number One 2 12 2 2 13 3 3 14 4 3 14 5 4 14 6 6 15 7 6 15 8 6 16 9 9 17 10 9 17 11 9 17 12 10 17 13 10 18 14 11 19 15 11 19 16 11 20 17 12 20 18 12 21 19 13 23 20 13 27 21 17 28 22 17 31 23 19 35 24 20 41 25 21 43 Mean ± Standard Deviation (sec) 10.24 ± 1.11 21.04 ± 1.70 ^*** * Significant deviation from control (*: p <0.05, **: p <0.01 and ***: p <0.001)

Experimental Example 5. Acute Toxicity Test

Acute toxicity testing of the extracts was performed using mice (20 ± 5 g, central laboratory animals) and rats (235 ± 10 g, central laboratory animals). ICR-based mice and Sprague-Dawley rats were divided into four groups of 10 rats each. Oral administration of the extracts of the present invention at doses of 250, 500, and 1000 mg / kg, respectively, was observed for 2 weeks. There were no cases of death in the group, and no symptoms were found.

The acute toxicity test showed that the LD ₅₀ was more than 1000 mg / kg for water extracts. In addition, no mortality was observed at the dose of 2000 mg / kg, and no significant abnormality was found in weight gain and feed intake. Therefore, the extract was found to be a safe drug.

Examples of the formulation of the composition are described below, but are not intended to limit the present invention but to explain in detail only.

Formulation Example 1 Preparation of Injection

Example 1 Dry Extract ............... 100 mg

Sodium metabisulfite ......................................... 3.0 mg

Methylparaben ...... 0.8 mg

Propylparaben ...................................... 0.1 mg

Sterile Distilled Water for Injection ...

The above ingredients were mixed and adjusted to 2 ml by a conventional method, and then filled into 2 ml ampoules and sterilized to prepare an injection.

Formulation Example 2 Preparation of Tablet

Example 1 Dry Extract ............... 200 mg

Lactose 100 mg

Starch ......................................... 100 mg

Magnesium Stearate ...............

The tablets were prepared by mixing the above components and tableting according to a conventional method for producing tablets.

Formulation Example 3 Preparation of Capsule

Example 1 Dry Extract ............... 100 mg

Lactose ...... 50 mg

Starch ............................ 50 mg

Talc ........................................ 2 mg

Magnesium Stearate ...............

The capsules were prepared by mixing the above components and filling the gelatin capsules according to a conventional method for preparing capsules.

Formulation Example 4 Preparation of Liquid

Example 1 Dry Extract ..................... 1000 mg

Sugar ........................... 20 g

Isomerized sugar ......................................... 20 g

Lemon scent .........................

Purified water was added to adjust the total volume to 100 ml.

The above-mentioned components were mixed according to a conventional method for preparing a liquid solution, filled into a 100 ml brown bottle, and sterilized to prepare a liquid solution.

In addition, health functional foods and health functional drinks were prepared in the following manner.

Formulation Example 5 Preparation of Health Functional Food

Example 1 Dry Powder ......................................... 1000 mg

Vitamin Blend ............... 20 g

Vitamin A Acetate ............... 70 μg

Vitamin E ......................... 1 mg

Vitamin B1 ..................................... 0.13 mg

Vitamin B2 ..................... 0.15 mg

Vitamin B6 ......................................... 0.5 mg

Vitamin B12 ................................. 0.2 μg

Vitamin C ......................................... 10 mg

Biotin .......................... 10 μg

Nicotinic Acid Amide ......................................... 1.7 mg

Folic acid ......................................... 50 μg

Calcium Pantothenate ......................................... 0.5 mg

Mineral mixture ...............

Ferrous Sulfate ............... 1.75 mg

Zinc Oxide ......................................... 0.82mg

Magnesium Carbonate ......................................... 25.3 mg

Potassium monophosphate ......................................... 15 mg

Dicalcium Phosphate ............... 55 mg

Potassium Citrate ......................................... 90 mg

Calcium Carbonate ... 100 mg

Magnesium Chloride ................................. 24.8 mg

Although the composition ratio of the vitamin and mineral mixture is a composition suitable for a relatively healthy food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health functional food production method. The granules may be prepared and used for preparing the nutraceutical composition according to a conventional method.

Formulation Example 6 Preparation of Health Functional Drink

Example 1 Dry Powder .......................... 1000 mg

Citric Acid ..................... 100 mg

Oligosaccharide ......................................... 100 g

Plum concentrate ..................... 2 g

Taurine ..................................... 1 g

Purified water is added.

After mixing the above components in accordance with a conventional healthy beverage production method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and then refrigerated and stored in the present invention It is used to prepare a health functional beverage composition.

Although the composition ratio is a composition suitable for a preferred beverage in a preferred embodiment, the compounding ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and use purpose.

As described above, the composite herbal extract of the present invention was confirmed to have an effect on enhancing memory capacity as a result of behavioral experiments of white rats through passive avoidance experiments, and by confirming the promotion of neural cell production in the hippocampus of the white rat brain, Extract of the present invention can be used as a composition or health functional food effective for improving learning ability and memory enhancement, prevention and treatment of dementia.

Claims (10)

A composition for enhancing memory, improving learning ability, and preventing and treating dementia, including a complex herbal extract comprising essentially Longanae Arillus, Zizyphi Spinosae Semen, Polygalae Radix, and Poria Cocos. The composition of claim 1, wherein the extract is extracted with one or more polar solvents selected from lower alcohols such as water, methanol, ethanol, and mixed solvents thereof. According to claim 1, in addition to the essential herbal medicine Angelicae Gigantis Radix, Ginseng Radix, Astragali Radix, Atractylodis Macrocephalae Rhizoma, Aucklandiae Radix, Glycyrrhizae Radix, Ginger (Zingiberis Rhizoma Recens) and control (Jujubae Fructus) composition comprising one or more herbs selected from the group consisting of. The composition of claim 1, wherein the longan meat, sanjoin, base paper, and baekxin are mixed at 5 to 25% by weight, respectively, based on the total weight. The composition according to claim 4, wherein the composition ratio of the long-term meat: sanjoin: base paper: baekxin is 1: 1: 1: 1. The composition according to claim 1, wherein the compound herbal compound includes longan meat, sanjoin, raw paper, white baeksin, donkey, ginseng, coriander, baekryeok, mokyang, licorice, ginger and a control. The compound according to claim 6, wherein the combined herbal is 5 to 25% by weight, 5 to 25% by weight, 5 to 25% by weight, 5 to 25% by weight of vaccine, 8 to 12% by weight, Angelica 8 to 12% by weight, ginseng 8, based on the total weight. To 12% by weight, 8 to 12% by weight, 8 to 12% by weight, 8 to 12% by weight, 4 to 8% by weight, licorice 2 to 6% by weight, 4 to 8% by weight and 4 to 8% by weight of ginger. . A health functional food comprising a compound herbal extract and a food-acceptable food supplement additive which essentially include the yongan meat, sanjoin, raw paper and baekxin extracts exhibiting memory enhancement, learning ability improvement and dementia prevention effect. 10. The health functional food according to claim 8, wherein the complex herbal medicine includes longan meat, sanjoin, raw paper, white baeksin, donkey, ginseng, astragalus, baekchul, mokyang, licorice, ginger and a control. The dietary supplement of claim 8 in the form of a powder, granule, tablet, capsule or beverage.