KR20020075033A - Complex enzyme for treatment of domestic aninal's excretions - Google Patents

Abstract

PURPOSE: A complex enzyme agent for treatment of feces and urine of domestic animals is provided, thereby the feces and urine of domestic animals can be easily and simply purified without dilution. CONSTITUTION: The complex enzyme agent for the treatment of feces and urine of domestic animals is produced by culturing Bacillus sp., Bacillus subtilis, Bacillus c., Penibacillus g., Cellulomonas c., Cellulomonas g., Bacillus caldoriticus, Penibacillus m., Aspergillus n., and Bacillus spp., in a medium containing 5mg of peptone, 3mg of meat extract, 20mg of agar and 1000ml of soil-dipped solution at 35 deg. C; inoculating the cultured medium into a second medium containing 1.3mg of ammonium sulfate, 0.37mg of K2HPO4, 0.25mg of MgSO4·7H2O, 0.07mg of calcium chloride, 0.02mg of FeCl3, 1.00mg of glucose, 1.00mg of yeast extract, 13.0mg of lactose and 1000ml of distilled water and culturing it; and inoculating the second cultured medium into a medium containing 80mg of soybean protein, 70mg of glucose, 50mg of potato, 60mg of NaCl and 1000ml of distilled water and culturing it for 72 hours with aeration.

Description

Complex enzyme agent for livestock manure processing {COMPLEX ENZYME FOR TREATMENT OF DOMESTIC ANINAL'S EXCRETIONS}

The present invention relates to a complex enzyme agent for livestock manure treatment that decomposes and treats livestock manure having a high BOD value.

Organic wastewater, such as livestock manure, causes pollution when discharged into rivers or rivers, so it must be treated chemically or purified by microbial treatment. In general, organic wastewater, especially wastewater such as phosphate, is formed during the storage of septic tanks, and microorganisms or protozoa naturally form and metabolize organic matter, resulting in the formation of gelatinous solids such as inorganic substances in sewage. Was formed and allowed to precipitate. This natural purification method takes a long time and does not have a complete treatment, so that the sewage discharged from the septic tank pollutes rivers or rivers. Recently, a method of treating sewage chemically or using an active microorganism has been developed. However, the above-described chemical treatment or microbial treatment produces good results in the BOD-low 200 ppm wastewater, but it has to be treated three times for complete treatment.

In particular, the treatment with microorganisms is intended to decompose only certain components in the sewage, so a great effect could not be expected for livestock manure, in which various kinds of organic substances are combined. Conventionally, wastewater such as livestock wastewater is treated with microorganisms in a second and third manner by diluting the wastewater after mechanical treatment for separating the fibers in one step. Therefore, a treatment agent capable of effectively purifying livestock wastewater as a single microbial treatment agent is required.

It is an object of the present invention to provide a complex enzyme agent which is a microbial treatment agent for livestock wastewater which can be treated quickly without diluting the livestock wastewater having a high BOD.

Objects of the present invention described above are Bacillus sp., Bacillus subtilis, Bacillus p., P. albacillus g., Cellulomonas p., Cellulomonas g., Bacillus caldorrylticus, P. nibacilli M., Aspergillus. Russ N., and Bacillus sp. It is achieved by the complex enzyme agent which selected and culture | cultivated and multiplyed 2 or more components in the inside.

The above-mentioned soil microorganisms are cultured as follows. The ground ground powder was dispersed in distilled water, sterilized under high pressure to prepare a soil leaching solution, and then, 1000 ml of this soil leaching solution was added with 5 mg of peptone, 3 mg of meat extract, and 20 mg of agar, and then mixed into a microorganism incubator. The above-mentioned microorganisms were added and incubated for 5 to 48 hours while maintaining the temperature of the incubator at 35 ° C, 1.3 mg of ammonium sulfate, 0.37 mg of potassium dihydrogen phosphate, 0.25 mg of magnesium sulfate heptahydrate, 0.07 mg of calcium chloride, and 2 chloride Transfer to a culture medium consisting of 0.02mg of iron, 1.00mg of glucose, 1.00mg of enzyme extract, 13.0mg of lactose, and 1000ml of distilled water, followed by secondary culture to allow the cells to multiply and produce enzymes. Soy protein 80mg, glucose 70mg, 50mg, The above-mentioned culture solution was added to a medium composed of 60 mg of NaCl and 1000 ml of distilled water, followed by culturing while injecting air for 72 hours to obtain the complex enzyme of the present invention.

According to the present invention, the protease, which is an enzyme used, is a proteolytic enzyme that breaks down the cell walls of fecal coliforms in wastewater and also decomposes pathogenic bacteria and rot bacteria, thereby making active proteins and helping to metabolize microorganisms. Sugars such as starch, glycogen, and dextrins are made into monosaccharides to promote carbohydrate decomposition and metabolism.Phosphatase breaks down phosphorus in organic matter in wastewater, while urease acts as urokinase in manure. Promote the decomposition of manure by producing it. In addition, lipase serves to decompose fatty acids in wastewater, cellulase plays a role of decomposing cellulose into monosaccharides, and phytase is a bacterium of Bacillus sp. It is an enzyme that is released during the cultivation of phytic acid, which does not come out of the digestive organs of animals, and breaks down into phosphorus and plays a role in precipitation. In addition, lipoxinase plays a role in decomposing fatty acid glycerides contained in organic matter into fatty acids.

According to the present invention, when the livestock manure is treated with a complex enzyme, polysaccharides, fatty acid esters and phosphorus compounds contained in the manure are decomposed into inorganic and transparent liquids, and thus the final treated inorganic material is precipitated and the wastewater becomes a colorless, odorless liquid. Has

Claims (1)

Bacillus sp., Bacillus subtilis, Bacillus c., Penibacilli g., Cellulomonas g., Cellulomonas g., Bacillus caldorticus, Penibacillus M., Aspergillus n., Bacillus sp. Incubated at a temperature of 35 ° C. in a medium composed of 5 mg of peptone, 3 mg of meat extract, 20 mg of agar and 1000 ml of soil leachate, 1.3 mg of ammonium sulfate, 0.37 mg of potassium dihydrogen phosphate, 0.25 mg of magnesium sulfate monohydrate, It was transferred to a culture medium composed of 0.07 mg of calcium chloride, 0.02 mg of ferric chloride, 1.00 mg of glucose, 1.00 mg of enzyme extract, 13.0 mg of lactose, and 1000 ml of distilled water, followed by secondary culture. Soy protein 80 mg, glucose 70 mg, 50 mg, NaCl 60 mg And a complex enzyme for livestock wastewater prepared by culturing while injecting the above-mentioned culture solution into a medium composed of 1000 ml of distilled water and injecting air for 72 hours.