KR20020040707A - Cosmetic composition containing ginsan polysaccharide extracted from panax ginseng - Google Patents

Abstract

PURPOSE: Provided is cosmetic composition containing ginsan polysaccharide extracted from panax ginseng which has anti-oxidation effects, recovering effects on damaged skin from contacting sun-lights and moisturizing effects on the skin. CONSTITUTION: Ginsan polysaccharide facilitates dermal cell proliferation and its biosynthesis. The cosmetic composition containing the ginsan polysaccharide is manufactured by the steps of: finely cutting panax ginseng then extracting it with water then freeze-drying the extract to obtain water-soluble powders; dissolving the powder in water then saturating it with ethanol to obtain pellets; dialyzing the pellets to obtain ginsan polysaccharide; and adding 0.001-10 wt.% of the polysaccharide as an active ingredient into cosmetic composition.

Description

Cosmetic composition containing ginseng extract polysaccharide jinsan {Cosmetic composition containing ginsan polysaccharide extracted from Panax ginseng}

The present invention relates to a cosmetic composition containing an antioxidant effect, ginseng extract polysaccharide acid, damaged skin recovery effect by light contact and skin moisturizing effect. More specifically, the present invention relates to a cosmetic composition containing ginseng extract polysaccharide carboxylic acid as an active ingredient, which has an effect of promoting the proliferation and biosynthesis of skin cells.

Ginseng contains fumaric acid, succinic acid, maleic acid, citric acid and tartaric acid as organic acids, oleic acid and linoleic acid as fatty acids, and paroxanes A to U as polysaccharides. In addition, pantothenic acid, choline, polyene, panasinol, heptadeca-ene-4,6-diyne-3,9-diol, oleanane, glycoside, alkaloid, and the like.

Among the components of ginseng, diol glycosides have an inhibitory effect on the central nervous system and have mental stability, analgesic, lowering blood pressure, and papaverine-like effects, and triolglycosides have an anti-fatigue and mental effect on the central nervous system. In addition to enhancing labor, panasinol and linoleic acid not only have anti-inflammatory effects, but also prevent the leakage of pigments in biological tissues invaded by X-ray irradiation, hypoglycemic activity, immunopharmacological activity, There is a repair effect of induced hepatic disorders, polyene is known to have an anti-cancer effect, promoting the biosynthesis of serum proteins.

Known techniques for cosmetics using ginseng extracts include Korean Patent Publication No. 99-86516, 'Cosmetic Composition Preventing Skin Aging Containing Ginseng Extract', and Korean Patent Publication No. 2001-88981 'Cosmetics Containing Ginseng Polysaccharide. Compositions' and the like. However, in the case of the cosmetic composition containing such ginseng extract it was difficult to show a special difference in effect compared to the conventional cosmetic composition.

The inventors of the present invention found that the ginseng extract polysaccharide different from the conventional ginseng extract has an excellent effect as a cosmetic, and named the ginseng extract polysaccharide Jinsan and then developed a cosmetic composition using the same as an active ingredient.

The technical problem to be achieved in the present invention is to develop a cosmetic composition containing ginseng extract polysaccharides acid with an antioxidant effect, damaged skin recovery effect by light contact and skin moisturizing effect. At this time, ginseng extract polysaccharides Jinsan ginseng root extract ginseng root, extracted with water and lyophilized to obtain a water-soluble powder, redissolved in water and precipitated with 80% ethanol to precipitate the ginseng extract prepared by dialysis It means polysaccharide.

1 is a process chart showing the extraction and purification process of ginseng extract polysaccharides Jinsan used in the cosmetic composition of the present invention.

An object of the present invention is to cut the dried ginseng root, extract with water and freeze-dried to obtain a water-soluble powder, and then redissolved in water and then precipitated with 70 ~ 90 (v / v)% ethanol to precipitate the material dialysis It is to provide a cosmetic composition having an antioxidant effect, damaged skin recovery effect by light contact, and skin moisturizing effect, comprising the ginseng extract polysaccharide prepared as 0.001 to 10% by weight active ingredient in the total weight of cosmetics.

In the cosmetic composition, the content of the ginseng extract polysaccharide is a cosmetic composition characterized in that it contains 0.01 to 1.0% by weight.

In addition, the cosmetic composition is characterized by having a cosmetic formulation, such as creams, lotions, emulsions or packs using conventional substrates and additives.

Hereinafter, the present invention will be described in more detail.

Looking at the manufacturing method of ginseng extract polysaccharides Jinsan used in the present invention. After ginseng root is thinly dried and extracted with water to obtain a water-soluble aliquot, it is lyophilized to obtain a water-soluble powder. The water-soluble powder refers to a ginseng extract polysaccharide prepared by dissolving in water and saturating with 70 to 90 (v / v)% ethanol to obtain a precipitate, followed by dialysis with water. The manufacturing process of the ginseng extract polysaccharide Jinsan of the present invention is shown in detail in FIG.

The ginseng polysaccharide of the present invention is easily blended with water, and fats and oils and organic solvents are difficult to blend with each other. When the ginseng polysaccharide of the present invention is blended into cosmetics, common ingredients such as oils, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, and preservatives may be applied as necessary. It is possible. In the present invention, the skin cosmetic is used for the skin, for example, may be in the form of a cosmetic, such as a lotion, latex, cream, pack, it is possible to apply to the skin anti-aging cosmetics.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the scope of the present invention is not limited by the following examples.

Preparation Example 1 Preparation of Ginseng Extract Polysaccharide

1 kg of 6-year-old ginseng is thinly dried and extracted with 8 L of water to obtain a water-soluble aliquot, and then lyophilized to obtain 180 g of a water-soluble powder. The water-soluble powder is redissolved in water, saturated with 80 (v / v)% ethanol to obtain a precipitate, and then dialyzed with water to prepare ginseng extract polysaccharide acid. The amount of the concentrated acid obtained was 90 g. The ginseng extract polysaccharide Jinsan prepared is a high molecular weight of about 30,000 to 150,000, and the main component of the acid contains polysaccharide (containing 35 to 60 wt%) and acidic sugar (containing 30 to 40 wt%) of galacturonic acid. It is.

Preparation Example 2 Preparation of Jinsan-Containing Lotion

To 8 g of 90% ethanol, 0.05 g of polyvinylpyrrolidone, 0.15 g of oleic acid alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of fragrance, 0.1 g of paraoxybenzoic acid methyl ester, a small amount of antioxidant, a small amount of pigment Dissolve. 0.2 g of jin acid prepared in Preparation Example 1, 5 g of glycerine was dissolved in 85.33 g of purified water, and then the mixed solution was added and stirred to obtain a lotion having a skin improvement effect.

Preparation Example 3 Preparation of Jinsan-Containing Cosmetic Suspension

1.2 g of cetyl alcohol, 10 g of squalene, 2 g of petrolatum, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monostearate, 1 g of polyoxyethylene (20 mole added) monooleate, and 0.1 g of fragrance were dissolved by heating at 70 ° C. 0.5 g of concentrated acid, 5 g of dipropylene glycol, 1500 g of polyethylene glycol, 0.2 g of triethanolamine, and 76.2 g of purified water are heated to 75 ° C to dissolve it. Both are emulsified by mixing, followed by cooling to obtain an emulsion having a water / oil-based skin improvement effect.

Preparation Example 4 Preparation of Jinsan-Containing Cosmetic Serum

To 5 g of 95% ethyl alcohol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of kitulose, 0.2 g of sodium hyaluronate, 0.2 g of vitamin E acetate, 0.2 g of sodium licorice, 0.1 g of paraoxybenzoic acid ethyl ester, jinsan 1g and an appropriate amount of pigments are mixed to obtain a cosmetic liquid with an effect of skin improvement.

Preparation Example 5 Preparation of Jinsan-Containing Lotion

Jinsan-containing lotion was prepared in the same manner as in Preparation Example 2, except that the acid content was changed to 0.5 g.

Preparation Example 6 Preparation of Jinsan-Containing Lotion

Jinsan-containing lotion was prepared in the same manner as in Preparation Example 2, except that the acid content was changed to 2.0 g.

Preparation Example 7 Preparation of Jinsan-Containing Lotion

Jinsan-containing lotion was prepared in the same manner as in Preparation Example 2, except that the acid content was changed to 3.0 g.

(Manufacture comparison example 1) Preparation of lotion

It was prepared in the same manner as in Preparation Example 2, except that 0.2 g of soluble starch was added instead of the acid.

(Manufacture comparison example 2) Preparation of lotion

It was prepared in the same manner as in Preparation Example 2 except that no acid was used.

(Manufacture comparison example 3) Preparation of lotion

It was prepared in the same manner as in Preparation Example 2 except that 0.2g of ginseng extract prepared by the method disclosed in Korean Patent Application Publication No. 99-86516 was added instead of Jinsan.

Example 1 Antioxidant Effect Test

Antioxidant effect was measured and tested using the lotion prepared in Examples 2 to 7 and Comparative Examples 1 to 3.

In the present invention, in order to measure the antioxidant effect, the effect of removing the active oxygen produced by xanthine and xanthinoxidase by the extract according to the present invention, that is, the active oxygen effect is evaluated. The amount of active oxygen is measured by generating active oxygen by xanthine and xanthine oxidase and reacting with nitrobluetetrazolium (NBT) to measure the blue color produced by this at a wavelength of 56 nm.

Test reagent:

A) 2.4 ml of 0.05 M Na ₂ CO ₃ buffer (molecular weight 105.99)

A solution of 5.25 g (50 mM) of sodium carbonate dissolved in purified water and a 50 mM NaHCO ₃ (molecular weight 84.01) solution are mixed to pH 10.2.

B) 0.1 ml of 3 mM xanthine solution

45.6 mg of xanthine (molecular weight 152.11) is dissolved in water to make 10 ml.

C) 0.1 ml of 3 mM EDTA solution

Sodium ethylenediamine tetraacetate (molecular weight 60.1) is dissolved in distilled water to make 3 mM.

D) 0.1 ml of 0.15% BSA solution

Dissolve 15mg of BSA (Fration V, Powder, Sigma) in distilled water to make 10ml.

E) 0.1 ml of 0.75 mM NBT solution

61.32 mg of nitroblue tetrazolium (molecular weight 817.65) is dissolved in distilled water to make 10 ml.

F) 0.1 ml xanthine oxidase solution

Dilute xanthine oxidase with distilled water about 100 times and allow the absorbance of the blank test to be in the range of 0.2 to 0.23 in the measurement procedure. Let the change in absorbance be A560 = 0.3 / 20 minutes.

G) 0.1 ml of 6 mM copper chloride solution

102.29 mg of copper chloride (molecular weight 134.55) is dissolved in distilled water to make 100 ml.

How to measure :

1) Add B, B, C, D, and B to Bayer's bottle, add 0.1 ml of sample solution to this, and let stand at 25 ℃ for 10 minutes.

2) Add a bar and stir quickly and incubate at 25 ° C for 20 minutes.

3) Then, add a yarn to stop the reaction and measure the absorbance St at 560nm.

4) In the blank test, absorbance Bt is measured by using distilled water instead of the sample solution as described above.

5) The blank test solution is operated in the same manner using distilled water instead of the bar to measure the absorbance Bo.

The antioxidant effect is calculated as follows.

Inhibition Rate (%) = (1- (St-So_ / Bt-Bo) × 100

St: absorbance at 560 nm after enzyme reaction of sample solution

Bt: absorbance at 560 nm after enzymatic reaction of the blank test solution

So: absorbance at 560 nm before reaction without enzyme addition of sample solution

Bo: absorbance at 560 nm before reaction without enzyme in blank test solution

The samples used in this experiment were all extracted and tested under the same conditions, and the average samples were tested with the original sample and the sample prepared by the company, which were known to have high antioxidant effects, using the sample stock solution and 3% aqueous solution of each sample. Table 1 shows the greatest antioxidant effect in the liquid extract of the present invention as a result of the antioxidant effect of each sample.

Sample name Inhibition Rate (%) Preparation Example 2 90.1 Preparation Example 5 92.2 Preparation Example 6 94.4 Preparation Example 7 93.5 Comparative Example 1 70.3 Comparative Example 2 67.8 Comparative Example 3 80.2

Example 2 Test for Measuring Damage to Damaged Skin by Optical Contact

After UV irradiation, the damaged skin recovery effect was measured by using a solar simulator. First, ultraviolet rays (UV) were irradiated to both arms of testers according to the FDA method to cause erythema.

After 8, 16, 24, and 48 hours after UV irradiation, Preparation Examples 2-7 and Comparative Examples 1-3 were applied to one arm of the tester, and distilled water was applied to the other arm. Skin color was measured before and after UV light. After UV irradiation, the whole skin color was adjusted to 100 and the erythema index was relatively measured. The Anova test statistical significance is p <0.05.

The effect of restoring skin damage after UV irradiation between Jinsan-containing lotion and the preparation lotion of Comparative Example (n = 10) Sample name Erythema index 8 hours 16 hours 24 hours 48 hours Preparation Example 2 127.18 ± 20.0 155.67 ± 15.9 148.63 ± 18.7 129.71 ± 28.7 Preparation Example 5 125.08 ± 15.2 148.72 ± 19.2 142.28 ± 17.9 126.25 ± 30.9 Preparation Example 6 123.47 ± 30.2 144.68 ± 31.5 138.67 ± 24.3 125.88 ± 26.1 Preparation Example 7 124.08 ± 18.5 145.04 ± 16.8 138.02 ± 19.1 126.06 ± 31.5 Comparative Example 1 150.78 ± 20.0 197.87 ± 20.9 195.35 ± 21.1 175.88 ± 26.3 Comparative Example 2 148.98 ± 22.0 195.78 ± 20.0 192.78 ± 20.0 174.11 ± 18.9 Comparative Example 3 136.42 ± 21.8 181.39 ± 22.1 160.78 ± 20.0 150.18 ± 26.3

Example 3 Skin Moisturizing Effect Measurement Test

Skin moisturizing effect was compared with hyaluronic acid, a moisturizer. Production Examples 2 to 7 and Comparative Examples 1 to 3 were applied by ten testers to the inside of the arm and taped. Skin hydration was obtained by measuring conductance before, immediately after and 2 hours after treatment.

In case of hyaluronic acid, the skin moisturizing rate decreased from 43% immediately after treatment to 12% after 2 hours of treatment, whereas for the acid-containing lotion prepared in Examples 2-7, the skin moisturizing rate was 43% immediately after treatment. After time it decreased to 11%. On the other hand, in the case of the lotion prepared in Comparative Examples 1 to 3, the skin moisturizing rate decreased from 43% immediately after treatment to 28% after 2 hours of treatment.

The effect of the present invention is to provide a cosmetic composition containing ginseng extract polysaccharides acid with an antioxidant effect, damage skin recovery effect by light contact and skin moisturizing effect. In addition, the cosmetic composition can be developed into a cosmetic formulation, such as cream, lotion, emulsion or pack using conventional substrates and additives.

Claims (3)

Ginseng roots were extracted by dipping, dried with water, lyophilized to obtain a water-soluble powder, redissolved in water, and then saturated with 70-90 (v / v)% ethanol to precipitate the ginseng extract. Cosmetic composition having an antioxidant effect, damage skin recovery effect by light contact, and skin moisturizing effect, comprising the polysaccharide as 0.001 to 10% by weight of the total active weight of cosmetics The cosmetic composition according to claim 1, wherein the content of the ginseng extract polysaccharide contains 0.01 to 1.0 wt%. Creams, lotions, emulsions or packs prepared using conventional substrates and additives in the cosmetic composition of claim 1