KR20020036264A - The Paste formulation of antagonistic microorganisms against gray molds - Google Patents

Abstract

PURPOSE: Provided is a process of preparing a paste increased in activity of an antagonistic microorganism, reinforced in medicinal efficacy and increased in duration thereof by increasing spreadability and fixability to leaves and stems of vegetables such as tomato. The paste has an increased controlling effect against gray mold. CONSTITUTION: A germicidal composition for controlling gray mold comprises 1% by weight of each Bacillus subtilis GB-0365(KFCC-11071) and Bacillus sp. GB-017(KFCC-11070) or a culture thereof as an antagonistic microorganism, 35% by weight of kaolin or loess, 35% by weight of elvan powder, 10% by weight of whole milk powder, 10% by weight of potato starch, 5% by weight of salt, 1% by weight of Na2HPO4·7H2O, 1% by weight of sodium alginate, 1% by weight of xanthan gum, 1% by weight of solid polyoxyethylene nonylphenyl ether and 1% by weight of sodium propionate.

Description

Coating agent for the control of gray mold disease containing the antagonist microbial culture as an active ingredient, a method for preparing the same and a method for controlling the gray mold disease using the same {The Paste formulation of antagonistic microorganisms against gray molds}

The present invention relates to a coating agent containing an antagonistic microorganism culture medium having a disease control ability as an active ingredient, a method for preparing the same, and a method for controlling plant diseases using the same.

In the long-term cultivation of plant vegetables, especially in winter, tomatoes are the most dangerous pests of productivity, the white fly, the gray mold that causes the stem. The greenhouse powder is an attractive insecticide and pyrethroid-based insecticide that can control adult insecticides such as buprofezin (trade name: Nogodan) and pyriproxyfen (trade name: Mirage) and adult insects. Essential control can be achieved by cross-spreading the pesticide.

On the other hand, Botrytis cinerea , a gray fungal pathogen of tomato, is a pathogen that is not easy to control, and is an incomplete fungus that grows at a temperature of 18 to 23 ° C. and grows well in a humid environment. Usually, these gray mold pathogens are infected with the target plants through the wounds caused by the aging of plants and the red leaves and harvesting when the low-temperature and humid greenhouse conditions are formed by starting heating. In particular, since these pathogens propagate better in their weak condition, the stems of tomatoes exposed to the weak condition in the greenhouse during the winter will have an easier environment for the development of the disease due to the increase in the number of latent bacteria. After all, the yield of tomatoes is inversely proportional to the rate of colonization caused by the ash fungus affecting its stems, and this high risk of disease, especially in greenhouse tomatoes, reduces the yield of tomatoes in good spring season, and causes huge losses for growers.

Therefore, many pesticides for controlling these diseases are currently on the market. However, spraying pesticides in greenhouses during the winter may rather encourage overheating of the greenhouse environment and, in some cases, lead to more infections in some cases. In order to prevent overheating of the greenhouse against this, some smoke is performed, but there are not many drugs available. On the other hand, attempts have been made to control the disease using antagonist microorganisms ( Cladosporium herbarua , Penicillium. Sp , Trichoderma harzianum , Bacillus subtilis ), but this also requires a lot of effort for practical use.

The present inventors also developed a microbial preparation for the control of gray mold using Bacillus subtilis GB-0365 (KFCC-11071) and GB-017 strain (KFCC-11070) (Korean Patent Application No. 98-54040), which are antagonistic strains. I've done it. However, despite the very strong activity of these strains on gray mold in the laboratory, the plant's foliar or stem surface is poorly settled, and in practice, the disease is often promoted when sprayed at a relatively low concentration. Occurred, and even when sprayed at high concentrations, the duration of the drug did not exceed 15 days.

In order to solve the above problems, the present invention contains the antagonist microbial culture as an active ingredient, by improving the electrodeposition and fixation of the foliar and stem surface of plants, especially vegetables, such as tomatoes, thereby improving the activity of the antagonist microorganisms It is an object of the present invention to provide a coating agent that increases its potency, enhances its efficacy and increases its duration.

The present invention also aims to provide a method for producing the coating agent.

Still another object of the present invention is to provide a method for controlling airborne fungal diseases of vegetables such as gray mold diseases. Hereinafter, the present invention will be described in more detail.

In order to achieve the above object, the present inventors include Bacillus subtilis GB-0365 (KFCC-11071) and GB-017 strain (KFCC-11070) (Korean Patent Application No. 98-54040), which are antagonists as active ingredients. , A coating agent for controlling diseases was further prepared, further comprising an agent for increasing the electrodeposition and fixability to the foliar and stem surface of the plant.

More specifically, the coating agent of the present invention as an active ingredient, the antagonist microorganism strains GB-0365 (KFCC-11071), GB-017 (KFCC-11070), respectively, 1%, the foliar and / or stem surface of the plant Kaolin (or ocher) 35%, elvan powder 35%, potato starch 10%, skim milk powder 10%; And as a component for increasing viscosity and increasing electrodeposition, sodium alginate 1%, solid polyoxyethylene nonylphenyl ether 1%; It contains 5% salt, 1% Na ₂ HPO ₄ · 7H ₂ O, and 1% sodium propionate for preservation and physical properties.

The coating agent of the present invention, when applied to the wound site of the plant prevented the secondary infection, especially when applied to the site of gray mold disease, not only showed an excellent therapeutic effect, but also the medicinal effect more than 30 days after application Lasted. This effect is not limited to gray mold, but has been shown to be widespread for airborne diseases such as layered rot.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention more specifically, and the scope of the present invention is not limited to these examples.

Example 1 Preparation of Antagonist Microbial Coatings

Bacillus subtilis GB-0365 (KFCC-11071) and GB-017 (KFCC-11070) were incubated according to the method described in Korean Patent Application No. 98-54040, and then mixed cultures were obtained in which they were mixed in a ratio of 1: 1. did. 100 ml of the mixed culture solution was sprayed onto a mixture of 350 g of kaolin (or ocher) and 350 g of elvan powder, followed by drying at 20 to 25 ° C. so as to have a water content of 20% or less. Next, 100 g of whole milk powder, 100 g of potato starch, 50 g of salt, 10 g of Na ₂ HPO ₄ · 7H ₂ O, 10 g of sodium alginate, 10 g of xanthan gum, 10 g of polyoxyethylene nonylphenyl ether, 10 g of sodium propionate was added and mixed and ground to prepare a total of 1 kg of the antagonist microbial coating agent. At the time of use, 1 kg of the coating agent was added to 10 L of water, mixed well, and used for 6 to 12 hours after being left in a dark and warm place.

Table 1. Composition of Antagonist Microbial Coating

material content Remarks Bacillus subtilis Culture medium Kaolin (or ocher) Elvan stone powder Battery Milk powder Potato salt Na ₂ HPO ₄ · 7H ₂ O Sodium alginate xanthan gum Polyoxyethylene Nonylphenyl etherSodium propionate 100ml350g350g100g100g50g10g10g10g10g10g Average particle size 2-7㎛ Average particle size 2-7㎛ sum 1 kg

Example 2: Control against Tomato Assay Fungus

1 kg of the antagonist microbial coating agent prepared according to Example 1 was mixed well in 10 L of water and then placed in a dark and warm place for 10 hours, and then tested after comparison with commercially available pesticides by treatment after cell work.

The test was carried out in a 6,000 pyeong (2,000 pyeong 3 dong) benro glass greenhouse in Hongcheon-gun, Gangwon-do, and 6,300 pyeong (3,000 1 dong, 3,300 pyeong 1 dong) benro glass greenhouse in Mungyeong, Gyeongbuk. As shown in Table 2, the coating agent of the present invention exhibited a very strong control power with about 98% control value and a low recurrence rate of 5% or less, and the drug lasted for about 30 days, so that the simple It shows superior control effect than culture treatment. In addition, it can be seen that, when treated using the microbial coating agent of the present invention, the effect was superior to the conventional chemical agent in control rate and relapse rate.

Table 2. Control of Antagonism Microbial Coatings against Tomato Blight Disease Against Chemical Pesticides

Experiment Control rate (%) Recurrence Rate (%) judgment 1-1-1 90.5 15.2 Smokers are good for keeping low humidity 1-1-2 80.5 29.4 Insufficient treatment effect 1-2-1 95.4 22.5 Scratch Problem 1-2-2 90.9 22.4 1-2-3 70.0 25.4 Need to use protective agent 2-1-1 95.1 20.8 The search 再 發 2-1-2 85.7 35.4 Severe 2-1-3 56.3 21.3 Poor efficacy, no residue 2-2-1 99.5 2.4 Very good effect 2-2-2 98.4 5.0

Each experimental section in Table 2 is as follows:

1-1 Smoke agent spraying: Smoke treatment 4 hours after sunset

1-1-1 Triflumizol (Trifumizol): a.i. per 400 m 3. Standard 5g

1-1-2 Chlorothalonil (trade name: Daconyl): a.i. per 400 m 3. Standard 5g

1-2 Foliar application of disinfectant: Add an electrodeposition agent in the morning on a sunny day and spray with a power sprayer

1-2-1 Iminoctadin (tris) albesiate (trade name: Befuran): 2,000 times

1-2-2 Triflumizol (Trifumizol): 2,000 times

1-2-3 GB-0365, GB-017 pure culture: 300 times

2-1 Local spraying of pesticides: 500 times of electrodeposition agent is applied to the infected area after surgery

2-1-1 triflumizol (Triflumizol): 1,000 times

2-1-2 Difenoconazol (trade name: Purgen) 1,000 times + Chlorothalonil (trade name: Daconyl): 400 times

2-1-3 copper hydroxide (Copper hydroxide): 800 times

2-2 Coating agent treatment of microbial agent: Applying the coating agent prepared in Example 1 with a brush

2-2-1: Applying infected area after surgery

2-2-2: Apply as is on infected area

The coating agent according to the present invention is greatly improved in the fixation and electrodeposition properties of the foliar and stem surface of the plant, when applied to a plant exposed to gray mold disease has the effect of long-term efficacy.

Claims (3)

Bacillus subtilis GB-0365 (KFCC-11071) and Bacillus sp. GB-017 (KFCC-11070) or cultures thereof, which are antagonistic microorganisms having control against airborne fungal diseases, are effective. A disinfectant for gray mold disease control. Bacillus subtilis GB-0365 (KFCC-11071) and Bacillus sp. GB-017 (KFCC-11070), or cultures thereof, which are antagonistic microorganisms having control against air infectious fungal diseases 1%, kaolin or ocher 35%, elvan powder 35%, whole milk powder 10%, potato starch 10%, salt 5%, Na ₂ HPO ₄ · 7H ₂ O 1%, sodium alginate 1%, xanthan gum 1%, solid phase A disinfectant composition for gray mold disease anti-foaming agent comprising 1% of polyoxyethylene nonylphenyl ether and 1% of sodium propionate. After culturing Bacillus subtilis GB-0365 (KFCC-11071) and Bacillus sp. GB-017 (KFCC-11070), which are antagonistic microorganisms having control against air infectious fungal disease, Obtaining a mixed culture solution mixed at a ratio of 1: 1; Spraying the mixed culture solution with a mixture of 35% kaolin or ocher and 35% elvan powder, and then drying the mixture to 20% or less at 20-25 ° C .; In the dried mixture, whole milk powder 10%, potato starch 10%, salt 5%, Na ₂ HPO ₄ · 7H ₂ O 1%, sodium alginate 1%, xanthan gum 1%, solid polyoxyethylene nonylphenyl ether (Polyoxyethylene Nonylphenyl ether) 1%, sodium propionate (Sodium propionate) 1% comprising the step of mixing and milling, a method for producing a fungicide composition for gray mold disease anti-foaming.